CN115244382A

CN115244382A - Sample analysis method and device

Info

Publication number: CN115244382A
Application number: CN202080098181.XA
Authority: CN
Inventors: 孙骁; 郭文恒; 司新春; 武振兴
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd; Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd; Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Priority date: 2020-04-13
Filing date: 2020-04-13
Publication date: 2022-10-25
Also published as: WO2021207897A1

Abstract

A sample analyzing method and apparatus for analyzing a sample by irradiating the measurement sample required for a sample and reagent preparation project with light of a plurality of wavelengths and a plurality of intensities to acquire optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, and selecting optical information corresponding to light of a specific wavelength and intensity among the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light according to an interferent content of the sample.

Description

Sample analysis method and device

Technical Field

The invention relates to a sample analysis method and a sample analysis device.

Background

A sample analysis apparatus is a type of apparatus for analyzing biochemical characteristics of a sample, which is widely used in the field of clinical medicine to help medical staff diagnose a patient's condition. Taking a hemagglutination instrument as an example, the hemagglutination instrument can measure the blood coagulation time and the concentration or activity of related substances contained in the blood; the coagulometer may detect coagulation items by an optical method, and specifically, the coagulometer irradiates light to a reaction cup solution during a reaction process and analyzes scattered or transmitted light to obtain optical information such as absorbance of the solution, so as to obtain coagulation time or concentration of a substance to be detected.

The optical method detects the coagulation item through optical information of scattering, reflection or transmission of light by the reaction solution, so that when the sample has an interfering substance, the nature of scattering, reflection or transmission of light by the reaction solution changes, which affects the measurement, and the detection result is inaccurate, and even cannot be obtained under severe conditions.

Summary of The Invention

Technical problem

The invention mainly provides a sample analysis method and a sample analysis device.

Solution to the problem

Technical solution

According to a first aspect, there is provided in an embodiment a method of sample analysis, comprising:

preparing a measuring sample required by the project through a sample and a reagent;

irradiating the measurement sample with light of a plurality of wavelengths and a plurality of intensities to acquire optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities;

and selecting optical information corresponding to the light with specific wavelength and intensity from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities according to the content of the interferent in the sample, and analyzing the sample.

In one embodiment, the selecting optical information corresponding to light with specific wavelength and intensity from the optical information corresponding to light with multiple wavelengths and multiple intensities according to the content of interferent in the sample, and analyzing the sample includes:

the items of the measured sample correspond to light with matched main wavelength and main intensity;

when the content of the interferent in the sample is smaller than a first threshold value, selecting optical information corresponding to the light with the main wavelength and the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample;

and when the content of the interferent in the sample is larger than a first threshold value, selecting optical information corresponding to light with the wavelength larger than the main wavelength corresponding to the item of the measured sample and/or the intensity larger than the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample.

In one embodiment, when the content of the interferent in the sample is greater than the first threshold and less than the second threshold, selecting optical information corresponding to light having a wavelength greater than a main wavelength corresponding to an item of the measurement sample and having an intensity equal to a main intensity corresponding to the item of the measurement sample from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, and analyzing the sample;

and when the content of the interferent in the sample is larger than a second threshold value, selecting optical information corresponding to light with a wavelength larger than a main wavelength corresponding to the item of the measurement sample and with an intensity larger than a main intensity corresponding to the item of the measurement sample from the optical information corresponding to the light with the plurality of wavelengths and the plurality of intensities to analyze the sample.

In one embodiment, when the content of the interfering substance in the sample is greater than the first threshold, it is further determined whether the item of the measurement sample supports the light of the non-dominant wavelength for measurement, and if not, the sample is analyzed by selecting the optical information corresponding to the light having the wavelength equal to the dominant wavelength corresponding to the item of the measurement sample and the intensity greater than the dominant intensity corresponding to the item of the measurement sample from the optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities.

In one embodiment, the plurality of wavelengths includes: a first dominant wavelength (405 nm) corresponding to a term measured by a chromogenic substrate method, a second dominant wavelength (575 nm) corresponding to a term measured by an immunoturbidimetric method, a third dominant wavelength (660 nm) corresponding to a term measured by a coagulation method, and a fourth wavelength (800 nm) greater than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength.

In one embodiment, the plurality of intensities includes at least a first intensity and a second intensity that is greater than the first intensity.

In one embodiment, the plurality of wavelengths and the plurality of intensities of light comprise: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a fourth wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity, light having a fourth wavelength and a second intensity.

According to a second aspect, there is provided in an embodiment a sample analysis device comprising:

a preparation section for preparing an assay sample required for a project by a sample and a reagent;

a light irradiation means for irradiating the measurement sample with light of a plurality of wavelengths and a plurality of intensities;

a light receiving unit for receiving an output optical signal of the measurement sample irradiated by the light irradiation unit to acquire optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities;

and the analysis component is used for selecting the optical information corresponding to the light with specific wavelength and intensity from the optical information corresponding to the light with multiple wavelengths and multiple intensities according to the content of the interferent in the sample to analyze the sample.

In one embodiment, the items of the test sample are determined to correspond to light having a dominant wavelength and a dominant intensity that are matched;

when the content of the interfering substance in the sample is less than a first threshold value, the analysis component selects optical information corresponding to the light with the main wavelength and the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample;

when the content of the interfering substance in the sample is greater than the first threshold, the analyzing unit selects optical information corresponding to light having a wavelength greater than the main wavelength corresponding to the item of the measurement sample and/or light having an intensity greater than the main intensity corresponding to the item of the measurement sample from among the optical information corresponding to the light having the plurality of wavelengths and the light having the plurality of intensities, and analyzes the sample.

In one embodiment, when the content of the interfering substance in the sample is greater than a first threshold and less than a second threshold, the analyzing component selects optical information corresponding to light having a wavelength greater than a dominant wavelength corresponding to an item of the measurement sample and having an intensity equal to a dominant intensity corresponding to the item of the measurement sample from among optical information corresponding to the plurality of wavelengths and the plurality of intensities of light to analyze the sample;

when the content of the interfering substance in the sample is greater than the second threshold, the analyzing unit selects optical information corresponding to light having a wavelength greater than a main wavelength corresponding to an item of the measurement sample and having an intensity greater than a main intensity corresponding to an item of the measurement sample from among the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, and analyzes the sample.

In one embodiment, when the content of the interfering substance in the sample is greater than the first threshold, the analyzing unit further determines whether or not the item of the measurement sample supports the light of the non-dominant wavelength for measurement, and if not, the analyzing unit selects the optical information corresponding to the light having a wavelength equal to the dominant wavelength corresponding to the item of the measurement sample and having an intensity greater than the dominant intensity corresponding to the item of the measurement sample from the optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities to analyze the sample.

In one embodiment, the plurality of wavelengths includes: a first dominant wavelength (405 nm) corresponding to a chromophoric substrate method measured item, a second dominant wavelength (575 nm) corresponding to an immunoturbidimetric method measured item, a third dominant wavelength (660 nm) corresponding to a coagulation method measured item, and a fourth wavelength (800 nm) not less than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength.

According to a third aspect, an embodiment provides a computer readable storage medium comprising a program executable by a processor to implement the method of any of the embodiments herein.

Advantageous effects of the invention

Brief description of the drawings

Drawings

FIG. 1 is a schematic diagram showing absorption spectra of three interferents including hemoglobin, bilirubin and chyle for light in each wavelength range;

FIG. 2 is a schematic representation of the transmission response optical curves for a normal sample and a severe chyle sample according to one embodiment;

FIG. 3 is a schematic structural diagram of a sample analyzer according to an embodiment;

FIG. 4 is a schematic structural view of a sample analyzer according to another embodiment;

FIG. 5 is a schematic view of the light provided by the illumination component during an illumination cycle;

FIG. 6 is another schematic view of the light provided by the illumination component during an illumination cycle;

FIG. 7 is a schematic view of an embodiment of a lighting unit;

FIG. 8 is a schematic view of a structure of a lighting unit according to another embodiment;

fig. 9 is a schematic structural view of a lighting unit according to still another embodiment;

fig. 10 is a schematic structural view of an illumination section of a further embodiment;

FIG. 11 is a schematic structural view of a sample analyzer according to still another embodiment;

FIG. 12 is a schematic flow chart of a sample analysis method according to an embodiment;

fig. 13 is a schematic flow chart of a sample analysis method according to another embodiment.

Examples of the invention

Modes for carrying out the invention

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments have been given like element numbers associated therewith. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the described features, operations, or characteristics may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The ordinal numbers used herein for the components, such as "first," "second," etc., are used merely to distinguish between the objects described, and do not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

Optical coagulation measurement can be generally classified into three methods: coagulation, immunoturbidimetry, and chromogenic substrate methods. The chromogenic substrate method generally employs violet light or ultraviolet light AT 340nm to 420nm, and is generally used for the measurement of detection items such as antithrombin-III (AT-III or AT 3). Immunoturbidimetry typically uses a 520nm-590nm yellow-green light, and immunoturbidimetry is typically used for assays such as D-dimer (DD), fibrin/Fibrinogen Degradation Products (FDP), and the like. The coagulation method generally employs red light or infrared light of 660nm to 800nm, and is generally used for measuring detection items such as thrombospondin time (PT), activated Partial Thromboplastin Time (APTT), thrombin Time (TT), and Fibrinogen (FIB).

In the optical measurement of blood coagulation, whether by coagulation, immunoturbidimetry or chromogenic substrate method, the presence of an interfering substance in a sample interferes with the detection. Normally, the plasma sample is light yellow and almost transparent, but some patients have symptoms of jaundice, hemolysis or lipemia due to diseases, and the like, so that the plasma is brownish yellow, reddish or milky. The symptom of jaundice indicates that bilirubin is an interferent in the sample, the symptom of hemolysis indicates that hemoglobin is an interferent in the sample, and the symptom of lipemia indicates that chyle is an interferent in the sample. The absorption spectra of the three interferents are different, and when interferents such as hemoglobin, bilirubin and chyle exist in sample plasma, the interferents can strongly absorb light, so that the detection of the sample is interfered, and the detection result is deviated. Hemoglobin, bilirubin, and chyle can generally be collectively referred to as HIL interference, where H refers to hemoglobin, I refers to bilirubin, and L refers to chyle. Bilirubin, hemoglobin and chyle, three interference substances present different colors, and absorption spectra thereof are shown in figure 1: bilirubin and hemoglobin have distinct absorption peaks, bilirubin has a strong absorption peak at about 450nm, hemoglobin has a strong absorption peak at about 420nm, and both have almost no absorption at a wavelength of 660nm or more, and chyle has absorbance in all visible spectra, and the absorbance decreases as the wavelength increases, but still has a certain absorbance even at 800nm. Therefore, as can be seen from the figure, the three interferents have strong absorption for light in a small wavelength band, especially light below 600nm, which greatly reduces the light transmittance of the mixture of the sample and the detection reagent, and the actually received light is very small, thereby affecting the accuracy and reliability of the optical method determination; sometimes the light that can be received is even almost zero, resulting in an inability to identify the reaction process of the sample with the detection reagent.

One solution to the interferents is to provide light in a wavelength band that is not absorbed by the interferents in the sample for detection, e.g., to provide a larger wavelength such as 800nm to illuminate the mixture of sample and detection reagent, as is evident from the figure, hemoglobin and bilirubin have little absorption of light at wavelengths greater than 800nm, while chyle has relatively little absorption of light at wavelengths greater than 800nm. The scheme can effectively eliminate the influence of bilirubin and hemoglobin on the sample determination, but has influence on the chylomicron sample, particularly the retrying of the chylomicron sample. Because although chyle has a relatively small absorption of light with a wavelength of more than 800nm, these absorptions of light by chyle are still not negligible for the case where the detection results require a relatively precise one; in addition, when the concentration of chyle in a sample is relatively high, even in a situation where the accuracy of the detection result requires general (for example, a physical examination test) the absorption of light by chyle is still not negligible, and chyle still seriously affects the accuracy of the detection result; the optical transmission response curves for the normal and severe chylomicron samples are shown in fig. 2, where the abscissa in fig. 2 is time in seconds and the ordinate is the light flux received by the transmission photodetector. As can be seen from FIG. 2, for the sample with severe chyle, due to its too low transmittance, the luminous flux of the reaction is almost zero all the time, and there is substantially no light transmission, i.e. the absorbance of the analyte is too large, exceeding the maximum absorbance test range of the optical sensor.

In addition, the above-mentioned scheme of measuring by switching light of a large wavelength is not applicable to items such as detection by a chromogenic substrate method, because the chromogenic substrate method is, in view of the detection principle, because a substance in a sample is replaced by a detection reagent after the sample and the detection reagent react, and the replaced substance absorbs only in the ultraviolet and violet ranges, it is generally possible to use only the above-mentioned violet light or ultraviolet light of 340nm to 420nm, and it is not possible to use light of other wavelength bands, and the coagulation method and immunoturbidimetric method can theoretically use light of other wavelength band ranges for detection in addition to the above-mentioned light of the respective wavelength band ranges.

The applicant researches and improves the problems, and proposes a dimension of increasing the light intensity to solve the influence of interferents on the optical detection; according to different contents of the interferent in the sample, the influence of the interferent on the sample detection is solved by selectively switching large wavelength and/or increasing light intensity. This will be explained in detail below.

In some embodiments of the invention, a sample analysis device is disclosed. A sample analysis device is an instrument for analyzing and measuring a sample. The flow of the test of the sample analyzer is not exemplified by a blood coagulation analyzer (i.e., a blood coagulation analyzer referred to herein). The test procedure for coagulation analyzers is generally as follows: the coagulation analyzer can irradiate the measurement sample in the cuvette with, for example, light of multiple wavelengths, and analyze the measurement sample by a coagulation method, an immunoturbidimetry method, a chromogenic substrate method, or the like to obtain a coagulation reaction curve of the measurement sample with time, thereby further calculating the coagulation time or other coagulation-related performance parameters of the measurement sample.

Referring to fig. 3, the sample analyzer in some embodiments includes a preparation unit 10, a light irradiation unit 30, a light receiving unit 50, and an analysis unit 70, which are described in detail below.

The preparation section 10 is used to prepare an assay sample required for a project by a sample and a reagent.

Fig. 4 is an embodiment of the preparation member 10, and the preparation member 10 may include a sample carrier member 11, a sample dispensing mechanism 12, a reagent carrier member 13, a reagent dispensing mechanism 14, and a reaction member 15. The sample carrier 11 is used to carry a sample. Some examples of the Sample carrier 11 may include a Sample Delivery Module (SDM) and a front end rail; in other examples, the sample carrier 11 may be a sample tray, such as the example shown in fig. 4, which may include a plurality of sample sites for placing samples, such as cuvettes, and the sample tray may be rotated to dispatch the samples to corresponding positions, such as positions for the sample dispensing mechanism 12 to aspirate the samples. The sample dispensing mechanism 12 is used for sucking a sample and discharging the sample into a reaction cup to be loaded. For example, the sample dispensing mechanism 12 may include a sample needle which is spatially moved in two or three dimensions by a two or three dimensional drive mechanism, so that the sample needle can be moved to aspirate a sample carried by the sample carrying member 11, and to a cuvette to be loaded, and discharge the sample to the cuvette. The reagent carrying member 13 is for carrying a reagent. In one embodiment, the reagent carrier 13 may be a reagent disk, the reagent disk is configured in a disk-shaped structure and has a plurality of positions for carrying reagent containers, and the reagent carrier 13 can rotate and drive the reagent containers carried by the reagent carrier to rotate to a specific position, for example, a position for sucking reagent by the reagent dispensing mechanism 14. The number of the reagent carrying members 13 may be one or more. The reagent dispensing mechanism 14 suctions and discharges a reagent into a reaction cuvette to be filled with the reagent. In one embodiment, the reagent dispensing mechanism 14 may include a reagent needle that performs a two-dimensional or three-dimensional motion in space by a two-dimensional or three-dimensional driving mechanism, so that the reagent needle can move to aspirate a reagent carried by the reagent carrying member 13 and to a cuvette to which the reagent is to be added and discharge the reagent to the cuvette. The reaction unit 15 is used to carry a container (e.g., cuvette) containing a measurement sample prepared from a sample and a detection reagent. In one example, the reaction component 15 is configured in a disc-shaped structure, and has a plurality of placing positions for placing, for example, reaction cups, and the reaction component 15 can rotate and drive the reaction cups in the placing positions to rotate for scheduling the reaction cups in the reaction tray and incubating a mixed solution in the reaction cups. The incubated assay sample is then dispensed to the sample detection site for optical detection. The sample detection site may be provided on the reaction part 15, i.e., some of the placement sites on the reaction part 15 are sample detection sites; the sample detection site may also be provided independently of the reaction part 15, i.e., at a position close to the reaction part 15, for example. There may be one or more sample detection sites.

The light irradiation section 30 is used to irradiate the measurement sample prepared by the preparation section 10 with light of various wavelengths and various intensities. The light of the above-described various wavelengths and various intensities will be explained below.

In some embodiments, the plurality of wavelengths includes: a first dominant wavelength corresponding to an item measured by a chromogenic substrate method, a second dominant wavelength corresponding to an item measured by an immunoturbidimetry method, a third dominant wavelength corresponding to an item measured by a coagulation method, and a fourth wavelength which is not less than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength. In some preferred embodiments, the first dominant wavelength is in the range of 340nm to 420nm, the second dominant wavelength is in the range of 520nm to 590nm, and the third dominant wavelength is in the range of 660nm to 800nm. In some embodiments, the fourth wavelength may take the value of 800nm.

In some embodiments, the plurality of intensities includes at least a first intensity and a second intensity greater than the first intensity.

Thus, in some embodiments, the multiple wavelengths and multiple intensities of light provided by the illumination component 30 include: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a fourth wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity, light having a fourth wavelength and a second intensity. The light may be time-shared to the sample detection site during a single illumination cycle, and thus used to illuminate the assay sample. One such example is illustrated in FIG. 5, where the first dominant wavelength is 405nm, the first dominant wavelength is 575nm, the third dominant wavelength is 660nm, and the fourth wavelength is 880nm.

In other embodiments, the light of various wavelengths and various intensities provided by the light illuminating component 30 includes: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity. The light may be time-shared to the sample detection site during a single illumination cycle, and used to illuminate the assay sample. One such example is shown in FIG. 6, where the first dominant wavelength is 405nm, the first dominant wavelength is 575nm, and the third dominant wavelength is 660nm.

For a sample analysis device provided with a plurality of sample detection sites, a one-to-many fiber optic bundle may be introduced to provide illumination to the plurality of sample detection sites for simplicity of construction. In some embodiments, referring to fig. 7, the illumination component 30 may include a light source 31 and a one-to-many optical fiber bundle 39, such that the light source 31 can provide light to a plurality of sample detection sites simultaneously through the one-to-many optical fiber bundle 39, and specifically, the one-to-many optical fiber bundle 39 includes a plurality of optical fibers respectively corresponding to the plurality of sample detection sites, each optical fiber being used for providing light of the plurality of wavelengths and the plurality of intensities to the corresponding sample detection site.

Referring to fig. 8, in some embodiments, the light source 31 may include a first light source 32, a second light source 33 and a third light source 34, the first light source 32 provides light with a first dominant wavelength, the second light source 33 provides light with a second dominant wavelength, the third light source 34 provides light with a third dominant wavelength, and the light source 31 may further include a fourth light source 35 for providing light with a fourth wavelength. It will be appreciated that, in order to improve the performance of the light, some optical components for focusing, such as a focusing lens, etc., may be further added between the light source 31 and the one-to-many fiber bundle 39; an associated collimating lens may also be added between the one-to-many fiber optic bundle 39 and each sample detection site to improve the performance of the light directed to the sample detection site. In some embodiments, the lighting assembly 30 further includes a driving circuit 36, the driving circuit 36 is connected to the first light source 32, the second light source 33, the third light source 34 and the fourth light source 35, and is configured to provide a first driving current to drive the first light source 32, the second light source 33, the third light source 34 and the fourth light source 35 to generate light with a first intensity; and also for providing a first driving current to drive the first light source 32, the second light source 33, the third light source 34 and the fourth light source 35 to generate light of a second intensity, the second driving current being greater than the first driving current. In this way, different driving currents are provided by the driving circuit 36 to drive the first light source 32, the second light source 33, the third light source 34 and the fourth light source 35, so that the illumination component 30 can provide light having the first dominant wavelength and the first intensity, light having the second dominant wavelength and the first intensity, light having the third dominant wavelength and the first intensity, light having the fourth wavelength and the first intensity, light having the first dominant wavelength and the second intensity, light having the second dominant wavelength and the second intensity, light having the third dominant wavelength and the second intensity, and light having the fourth wavelength and the second intensity in a time-sharing manner within one illumination period.

Referring to fig. 9, in other embodiments, the light source 31 may also be implemented by a multi-wavelength light source 37 and a rotating filter 38. The multi-wavelength light source 37 provides light at multiple wavelengths, such as a first dominant wavelength of light, a second dominant wavelength of light, a third dominant wavelength of light, and a fourth wavelength of light. In some examples, the multi-wavelength light source 37 may be implemented using a halogen lamp. The rotating filter 38 includes a filter and an attenuating plate, and the light irradiation part 30 is used for providing light with different wavelengths and different intensities in a time sharing manner when the rotating filter 38 rotates, for example, providing light with a first main wavelength and a first intensity in a time sharing manner, providing light with a second main wavelength and a first intensity in a time sharing manner, providing light with a third main wavelength and a first intensity in a time sharing manner, providing light with a fourth wavelength and a first intensity in a time sharing manner, providing light with a first main wavelength and a second intensity in a time sharing manner, providing light with a second main wavelength and a second intensity in a time sharing manner, providing light with a third main wavelength and a second intensity in a time sharing manner, and providing light with a fourth wavelength and a second intensity in a time sharing manner. The light may then be provided to a plurality of sample detection sites via a one-to-many fiber optic bundle 59. It will be appreciated that optical components, such as lens assemblies, may be added between the multi-wavelength light source 37 and the rotary filter 38 to improve the illumination performance.

The light period referred to herein may be 0.1s.

In some examples, the sample analysis device also provides interferent detection sites, as further described below. The illumination means 30 may also provide illumination to the interfering object detection site, for example, by a one-to-many fiber bundle 59, so that the illumination of the sample detection site and the interfering object detection site by the illumination means 30 is the same, as shown in fig. 10 as an example. In the figure, the violet LED provides 405nm light, the green LED provides 575nm light, the red LED provides 660nm light, the infrared LED provides 800nm light, and they are coupled together into the optical fiber of the one-component optical fiber bundle 59 by three dichroic mirror bundles, the one-component optical fiber bundle 59 is divided into a plurality of small fiber ends, one of which can be used to illuminate a second container (such as a cuvette or a reaction cup) at the interference detection position for detecting the type and content of the interferent in the sample, for example, the interferent, and the other small fiber ends are used to illuminate a container (such as a reaction cup) which is located at the sample detection position and contains the measurement sample. In some examples, the illumination component 30 may also independently provide illumination to the sample detection site and the interferent detection.

The above are some of the descriptions of the illuminating member 30. The light receiving unit 50 is coupled to the light irradiating unit 30, and the light receiving unit 50 is configured to receive output optical signals, such as reflected light, refracted light, transmitted light, and the like, of the measurement sample irradiated by the light irradiating unit 30, so as to acquire optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities. In one embodiment, the light receiving unit 50 is a light receiving unit that receives light irradiated from the light irradiation unit 30 and transmitted through the measurement sample, thereby acquiring optical information corresponding to the plurality of wavelengths and the plurality of intensities of light.

In some embodiments, the light receiving part 50 may be implemented by an electro-optical part capable of converting an optical signal into an electrical signal. Such optoelectronic components may in particular be photodiodes PD, photomultiplier tubes PMT, avalanche photodiodes APD, charge coupled devices CCD, complementary metal oxide semiconductor CMOS, image enhanced detectors ICCD or electron multiplying EMCCD. For sample analysis devices provided with a plurality of sample detection sites, one opto-electronic component may be provided for each sample detection site.

The analysis component 70 is configured to select optical information corresponding to light with a specific wavelength and intensity from the optical information corresponding to light with the plurality of wavelengths and the plurality of intensities according to the content of the interferent in the sample, and analyze the sample. Two links are involved, namely how to obtain the content of interferent in the sample, and how to select optical information corresponding to light with specific wavelength and intensity to analyze the sample, which are respectively explained below.

The following is a description of how to obtain and determine the interferent content of the sample.

The interference content of the sample can be judged through sample interferent detection information. In some embodiments, the sample interferent detection information comprises at least one of absorbance or luminous flux of the sample to be detected; the absorbance of the sample to be detected represents the absorption degree of the sample to be detected to light when the sample to be detected is irradiated by light; if the absorbance of the sample to be detected to the preset wavelength exceeds a preset absorbance threshold, for example, the absorbance of at least one of 405nm, 575nm, 660nm and 800nm exceeds a corresponding absorbance threshold, it indicates that the interferent of the sample to be detected exceeds the preset threshold, and anti-interference detection needs to be performed by increasing the light intensity or other methods; the luminous flux of the sample to be detected represents the degree of light passing through the sample to be detected when the sample to be detected is irradiated by the light, and the luminous flux of the sample to be detected can be the initial luminous flux detected before the sample to be detected is subjected to formal coagulation project detection; if the initial luminous flux of the sample to be detected is lower than a preset luminous flux threshold value, the fact that the interferent of the sample to be detected exceeds the preset threshold value is indicated, and anti-interference detection needs to be carried out in a mode of improving light intensity.

This is illustrated below by some specific examples. Referring to fig. 11, in some embodiments, the sample analyzer may further include an interferent detection unit 80. In some embodiments, the interferent detection means 80 comprises at least one interferent detection bit and a detector 81 adjacent to the interferent detection bit. The detector 81 may be implemented by a component capable of converting an optical signal into an electrical signal, such as a photodetector or the like, and specifically may be a photodiode PD, a photomultiplier tube PMT, an avalanche photodiode APD, a charge coupled device CCD, a complementary metal oxide semiconductor CMOS, an image enhancement type detector ICCD, an electron multiplication type EMCCD, or the like. The illumination means 30 is for illuminating a second container (e.g., a cuvette, or the like) containing at least the sample at the interfering substance detection site — for example, the illumination means 30 is illuminated by light of a first intensity; the detector 81 is configured to receive an output optical signal of the second container irradiated by the illumination component 30 to obtain detection information of an interfering object of the sample to be detected; the interferent detection information is used for indicating the interferent content of the sample to be detected.

The introduction of the interference detection component 80 can detect the interferent in the sample to be detected, and obtain the detection information of the sample interferent. In other embodiments, the interferent detection site may not be separately provided, but the interferent detection on the sample to be detected may be implemented while the sample is detected at the sample detection site, which is described in detail below.

In some embodiments, the light-emitting component 30 emits light with a first intensity to a container containing a measurement sample at a sample detection position, and the light-receiving component 50 is configured to receive an output optical signal of the container after being emitted by the light-emitting component 30, so as to obtain detection information of an interfering object of the measurement sample; the interferent detection information is used for judging the content of interferents in the sample to be detected. Specifically, the interfering substance detection information may be acquired using the average light flux of the time period after the container containing the measurement sample is placed at the sample detection position and before the start of the test. In one example, when the sample is added to the last step of the trigger reagent for starting timing, the mixing and moving of the mixture to the sample detection position can be completed within generally 3s, then the detection is started at the 10 th s, and at 7s between the 3 rd s and the 10 th s, the light irradiation part 30 irradiates a container containing the measurement sample at the sample detection position with light of the first intensity, and the light receiving part 50 is used for receiving an output light signal of the container irradiated by the light irradiation part 30, for example, the minimum light transmission amount of the average light flux in the period of time, so as to obtain the detection information of the interferent of the sample.

In the above embodiments of detecting a sample interfering substance, it can be understood that a person skilled in the art may also use other methods to detect a sample interfering substance, for example, by taking a picture of a sample to be detected, obtaining an image of the sample to be detected, and then analyzing the image by a method such as machine learning, so as to obtain the detecting information of the sample to be detected.

Next, how the analyzing part 70 selects the optical information analysis sample corresponding to the light of the specific wavelength and intensity will be described.

The items of the measurement sample correspond to light having a dominant wavelength and a dominant intensity that match each other. For example, the dominant wavelength for the items measured by the chromogenic substrate method is the first dominant wavelength in the above, the dominant wavelength for the items measured by the immunoturbidimetric method is the second dominant wavelength in the above, the dominant wavelength for the items measured by the coagulation method is the third dominant wavelength in the above, and the like, and the dominant intensities thereof are the first intensities.

When the content of the interfering substance in the sample is less than the first threshold value, the analyzing section 70 selects optical information corresponding to light of a main wavelength and a main intensity corresponding to the item of the measurement sample from among optical information corresponding to light of the plurality of wavelengths and the plurality of intensities to analyze the sample.

When the content of the interfering substance in the sample is greater than the first threshold, the analyzing section 70 selects, from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, the optical information corresponding to the light having a wavelength greater than the dominant wavelength corresponding to the item of the measurement sample and/or having an intensity greater than the dominant intensity corresponding to the item of the measurement sample, and analyzes the sample. For example, taking the item of immunoturbidimetric assay as an example, when the content of the interfering substance in the sample is greater than the first threshold, the analyzing unit 70 may select the optical information corresponding to the light with the fourth wavelength and the first intensity to analyze the sample, may select the optical information corresponding to the light with the second dominant wavelength and the second intensity to analyze the sample, and may even select the optical information corresponding to the light with the fourth wavelength and the second intensity to analyze the sample.

In some embodiments, when the content of the interferent in the sample is greater than the first threshold, the method may be further subdivided into two cases, that is, the content of the interferent in the sample is greater than the first threshold and less than the second threshold, and the content of the interferent in the sample is greater than the second threshold, which will be described in detail below.

When the content of the interfering substance in the sample is greater than the first threshold value and less than the second threshold value, the analyzing unit 70 selects, from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, the optical information corresponding to the light having a wavelength greater than the dominant wavelength corresponding to the item of the measurement sample and having an intensity equal to the dominant intensity corresponding to the item of the measurement sample, and analyzes the sample. Taking the immunoturbidimetric assay as an example, when the interferent content of the sample is greater than the first threshold value and less than the second threshold value, the analyzing unit 70 may select the optical information corresponding to the light with the fourth wavelength and the first intensity to analyze the sample.

When the content of the interfering substance in the sample is greater than the second threshold, the analyzing unit 70 selects, from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, the optical information corresponding to the light having a wavelength greater than the main wavelength corresponding to the item of the measurement sample and having an intensity also greater than the main intensity corresponding to the item of the measurement sample, and analyzes the sample. Still taking the immunoturbidimetric assay as an example, when the interferent content of the sample is greater than the second threshold, the analyzing component 70 may select the optical information corresponding to the light of the fourth wavelength and the second intensity to analyze the sample.

When the content of the interferent in the sample is greater than the first threshold and less than the second threshold, selecting the optical information analysis sample corresponding to the light with the original light intensity and the large wavelength, and when the content of the interferent in the sample is greater than the second threshold, selecting the optical information analysis sample corresponding to the light with the large light intensity and the large wavelength, which is of practical significance, because if the content of the interferent in the sample is greater than the first threshold and less than the second threshold, the optical information analysis sample corresponding to the light with the large light intensity and the large wavelength is selected, the concentration of the interferent in the sample is not very high, the optical signal received by the light receiving component 50 after the light with the large light intensity and the large wavelength irradiates the measurement sample may be supersaturated, and adversely affects the analysis sample; similarly, if the optical information corresponding to the original light intensity and the light with a large wavelength is selected to analyze the sample when the content of the interferent in the sample is greater than the second threshold, since the interferent concentration in the sample is very high, the intensity of the light signal that may be received by the light receiving unit 50 after the original light intensity and the light with a large wavelength irradiate the measurement sample is very small, which adversely affects the analysis sample.

As described above, there are some items that do not support measurement of light having a wavelength other than the dominant wavelength, and typically, like the item of the chromogenic substrate method, measurement is performed only by light having the first dominant wavelength, and measurement cannot be performed by light having a wavelength that is switched to a longer wavelength. In view of this, in some embodiments, when the content of the interfering substance in the sample is greater than the first threshold, the analyzing unit 70 further determines whether the item of the measurement sample supports the non-dominant wavelength light for measurement, and if not, the analyzing unit 70 selects the optical information corresponding to the light having the wavelength equal to the dominant wavelength corresponding to the item of the measurement sample and the intensity greater than the dominant intensity corresponding to the item of the measurement sample from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light to analyze the sample. Taking the item of measurement by the chromogenic substrate method as an example, when the content of the interfering substance in the sample is greater than the first threshold value, the analyzing section 70 judges that the item does not support the light of the non-dominant wavelength for measurement, and therefore the analyzing section 70 selects the optical information corresponding to the light of the second wavelength and the second intensity to analyze the sample.

The above is a description of a sample analysis device according to some embodiments of the present invention. The invention also discloses a sample analysis method in some embodiments.

Referring to fig. 12, the sample analysis method in some embodiments includes the following steps:

step 100: the measurement samples required for the items are prepared by the sample and the reagent.

Step 200: irradiating the measurement sample with light of a plurality of wavelengths and a plurality of intensities to acquire optical information corresponding to the plurality of wavelengths and the plurality of intensities of light.

In some embodiments, the plurality of wavelengths involved in step 200 includes: a first dominant wavelength corresponding to a project measured by a chromogenic substrate method, a second dominant wavelength corresponding to a project measured by an immunoturbidimetric method, a third dominant wavelength corresponding to a project measured by a coagulation method, and a fourth wavelength which is not less than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength. In some preferred embodiments, the first dominant wavelength is in the range of 340nm to 420nm, the second dominant wavelength is in the range of 520nm to 590nm, and the third dominant wavelength is in the range of 660nm to 800nm. In some embodiments, the fourth wavelength may take the value of 800nm.

In some embodiments, the plurality of intensities involved in step 200 includes at least a first intensity and a second intensity that is greater than the first intensity.

Thus, in some embodiments, the plurality of wavelengths and the plurality of intensities of light provided in step 200 include: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a fourth wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity, light having a fourth wavelength and a second intensity. The light may be time-shared to the sample detection site during a single illumination cycle, and thus used to illuminate the assay sample. An example of this is shown in figure 5 above.

In other embodiments, the plurality of wavelengths and the plurality of intensities of light provided in step 200 include: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity. The light may be time-shared to the sample detection site during a single illumination cycle, and used to illuminate the assay sample.

Step 300: and selecting optical information corresponding to the light with specific wavelength and intensity from the optical information corresponding to the light with multiple wavelengths and multiple intensities according to the content of the interferent in the sample, and analyzing the sample. How to detect the content of the interferent in the sample is described in detail above, and is not described herein again. The following describes how step 300 selects an optical information analysis sample corresponding to light of a specific wavelength and intensity.

When the content of the interferent in the sample is less than the first threshold, step 300 selects the optical information corresponding to the light with the dominant wavelength and the dominant intensity corresponding to the item of the measurement sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample.

When the content of the interferent in the sample is greater than the first threshold, step 300 selects, from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, the optical information corresponding to the light having the wavelength greater than the dominant wavelength corresponding to the item of the measurement sample and/or the light having the intensity greater than the dominant intensity corresponding to the item of the measurement sample to analyze the sample. For example, taking the item of immunoturbidimetric assay as an example, when the content of the interfering substance in the sample is greater than the first threshold, the analyzing unit 70 may select the optical information corresponding to the light with the fourth wavelength and the first intensity to analyze the sample, may select the optical information corresponding to the light with the second dominant wavelength and the second intensity to analyze the sample, and may even select the optical information corresponding to the light with the fourth wavelength and the second intensity to analyze the sample.

When the content of the interferent in the sample is greater than the first threshold and less than the second threshold, step 300 selects the optical information corresponding to the light having a wavelength greater than the main wavelength corresponding to the item of the measurement sample and having an intensity equal to the main intensity corresponding to the item of the measurement sample from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light to analyze the sample. Still taking the immunoturbidimetric assay as an example, when the interferent content of the sample is greater than the first threshold and less than the second threshold, step 300 may select the optical information corresponding to the light of the fourth wavelength and the first intensity to analyze the sample.

When the content of the interferent in the sample is greater than the second threshold, step 300 selects the optical information corresponding to the light with the wavelength greater than the main wavelength corresponding to the item of the measurement sample and the intensity greater than the main intensity corresponding to the item of the measurement sample from the optical information corresponding to the light with the plurality of wavelengths and the plurality of intensities to analyze the sample. Still referring to the immunoturbidimetric assay, when the interferent content of the sample is greater than the second threshold, step 300 may select optical information corresponding to the light of the fourth wavelength and the second intensity to analyze the sample.

It is practical to select the optical information analysis sample corresponding to the original light intensity and the light with large wavelength when the content of the interferent in the sample is greater than the first threshold and less than the second threshold, and to select the optical information analysis sample corresponding to the light with large light intensity and large wavelength when the content of the interferent in the sample is greater than the second threshold, because the interferent concentration in the sample is not very high, the optical signal received by the light receiving part 50 after the light with large light intensity and large wavelength irradiates the measurement sample may be oversaturated, and adversely affect the analysis sample; similarly, if the optical information corresponding to the original light intensity and the light with a large wavelength is selected to analyze the sample when the content of the interfering substance in the sample is greater than the second threshold, since the concentration of the interfering substance in the sample is very high, the intensity of the light signal that may be received by the light receiving element 50 after the original light intensity and the light with a large wavelength irradiate the measurement sample is very small, which may adversely affect the analysis sample.

As described above, there are some items that do not support measurement of light having a wavelength other than the dominant wavelength, and typically, like the item of the chromogenic substrate method, measurement is performed only by light having the first dominant wavelength, and measurement cannot be performed by light having a wavelength that is switched to a longer wavelength. In view of this, in some embodiments, when the content of the interferent in the sample is greater than the first threshold, step 300 further determines whether the item of the measurement sample supports measurement with light of a non-dominant wavelength, and if not, step 300 selects, from the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, optical information corresponding to light having a wavelength equal to the dominant wavelength corresponding to the item of the measurement sample and an intensity greater than the dominant intensity corresponding to the item of the measurement sample, and analyzes the sample. Taking the item of measurement by the chromogenic substrate method as an example, if the content of the interfering substance in the sample is greater than the first threshold, step 300 determines that the item does not support the light of the non-dominant wavelength for measurement, and therefore step 300 selects the optical information corresponding to the light of the second wavelength and the second intensity to analyze the sample.

In summary, referring to fig. 13, in an embodiment of the sample analysis method, the method may include the following steps:

Step 310: and judging whether the content of the interferent in the sample is less than a first threshold value.

Step 312: and when the content of the interferent in the sample is less than a first threshold value, selecting optical information corresponding to the light with the main wavelength and the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample.

Step 314: and when the content of the interferent in the sample is larger than the first threshold value, continuously judging whether the item of the measurement sample supports the light with the non-dominant wavelength for measurement. If the item of the measurement sample does not support the measurement with the light of the non-dominant wavelength, the step 316 is performed, whereas if the item of the measurement sample supports the measurement with the light of the non-dominant wavelength, the step 318 is performed.

Step 316: when the item of the measurement sample does not support the light of the non-dominant wavelength for measurement, the sample is analyzed by selecting the optical information corresponding to the light of the wavelength equal to the dominant wavelength corresponding to the item of the measurement sample and the intensity greater than the main intensity corresponding to the item of the measurement sample from the optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities.

Step 318: when the item of the measurement sample supports the measurement of light with non-dominant wavelength, whether the content of the interferent in the sample is larger than a second threshold value is further judged.

Step 320: and when the content of the interferent in the sample is larger than or equal to the second threshold, selecting optical information corresponding to light with the wavelength larger than the main wavelength corresponding to the item of the measured sample and the intensity equal to the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the plurality of wavelengths and the plurality of intensities to analyze the sample.

Step 322: and when the content of the interferent in the sample is larger than a second threshold value, selecting optical information corresponding to light with a wavelength larger than the main wavelength corresponding to the item of the measured sample and with an intensity larger than the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the plurality of wavelengths and the plurality of intensities to analyze the sample.

Reference is made herein to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications may be made to the exemplary embodiments without departing from the scope hereof. For example, the various operational steps, as well as the components for performing the operational steps, may be implemented in differing ways depending upon the particular application or consideration of any number of cost functions associated with the operation of the system (e.g., one or more steps may be deleted, modified, or combined with other steps).

In the above embodiments, all or part of the implementation may be realized by software, hardware, firmware, or any combination thereof. Additionally, as will be appreciated by one skilled in the art, the principles herein may be reflected in a computer program product on a computer readable storage medium having computer readable program code pre-loaded thereon. Any tangible, non-transitory computer-readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-to-ROM, DVD, blu-Ray discs, etc.), flash memory, and/or the like. These computer program instructions may be loaded onto a general purpose computer, special purpose computer, or other programmable data processing apparatus to produce a machine, such that the instructions which execute on the computer or other programmable data processing apparatus create means for implementing the functions specified. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including means for implementing the function specified. The computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified.

While the principles herein have been illustrated in various embodiments, many modifications of structure, arrangement, proportions, elements, materials, and components and otherwise, used in the practice of the disclosure, which are particularly adapted to specific environments and operative requirements, may be employed without departing from the principles and scope of the present disclosure. The above modifications and other changes or modifications are intended to be included within the scope of this document.

The foregoing detailed description has been described with reference to various embodiments. However, one of ordinary skill in the art would recognize that various modifications and changes can be made without departing from the scope of the present disclosure. Accordingly, the disclosure is to be considered in all respects as illustrative and not restrictive, and all such modifications are intended to be included within the scope thereof. Also, advantages, other advantages, and solutions to problems have been described above with regard to various embodiments. However, the benefits, advantages, solutions to problems, and any element(s) that may cause any element(s) to occur or become more pronounced are not to be construed as a critical, required, or essential feature or element of any or all the claims. As used herein, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, system, article, or apparatus. Furthermore, the term "coupled," and any other variation thereof, as used herein, refers to a physical connection, an electrical connection, a magnetic connection, an optical connection, a communicative connection, a functional connection, and/or any other connection.

Those skilled in the art will recognize that many changes may be made to the details of the above-described embodiments without departing from the underlying principles of the invention. Accordingly, the scope of the invention should be determined only by the claims.

Claims

A method of analyzing a sample, comprising:

preparing a measurement sample required by the project through a sample and a reagent;

irradiating the measurement sample with light of a plurality of wavelengths and a plurality of intensities to acquire optical information corresponding to the plurality of wavelengths and the plurality of intensities of light;

and selecting optical information corresponding to the light with specific wavelength and intensity from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities according to the content of the interferent in the sample, and analyzing the sample.
The method for analyzing a sample according to claim 1, wherein the selecting optical information corresponding to light of a specific wavelength and intensity from the optical information corresponding to light of the plurality of wavelengths and the plurality of intensities according to the interferent content of the sample, and analyzing the sample comprises:

the items of the measured sample correspond to light with matched main wavelength and main intensity;

when the content of the interferent in the sample is smaller than a first threshold value, selecting optical information corresponding to the light with the main wavelength and the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample;

and when the content of the interferent in the sample is larger than a first threshold value, selecting optical information corresponding to light with the wavelength larger than the main wavelength corresponding to the item of the measured sample and/or the intensity larger than the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample.
The method for analyzing a sample according to claim 2, wherein when the content of the interfering substance in the sample is greater than a first threshold value and less than a second threshold value, the sample is analyzed by selecting optical information corresponding to light having a wavelength greater than a dominant wavelength corresponding to an item of the measurement sample and having an intensity equal to a dominant intensity corresponding to an item of the measurement sample, from among the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light;

and when the content of the interferent in the sample is larger than a second threshold value, selecting optical information corresponding to light with a wavelength larger than the main wavelength corresponding to the item of the measured sample and with an intensity larger than the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the plurality of wavelengths and the plurality of intensities to analyze the sample.
The sample analysis method according to claim 2 or 3, wherein when the content of the interfering substance in the sample is greater than the first threshold, it is further determined whether or not the item of the measurement sample supports light of a non-dominant wavelength for measurement, and if not, the sample is analyzed by selecting optical information corresponding to light having a wavelength equal to the dominant wavelength corresponding to the item of the measurement sample and having an intensity greater than the dominant intensity corresponding to the item of the measurement sample, from among the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light.
The sample analysis method of claim 1, wherein the plurality of wavelengths comprises: a first dominant wavelength (405 nm) corresponding to a chromogenic substrate method measurement item, a second dominant wavelength (575 nm) corresponding to an immunoturbidimetric method measurement item, a third dominant wavelength (660 nm) corresponding to a coagulation method measurement item, and a fourth wavelength (800 nm) greater than the first, second, and third dominant wavelengths.
The method for analyzing a sample according to claim 1 or 5, wherein the plurality of intensities comprises at least a first intensity and a second intensity greater than the first intensity.
The sample analysis method of claim 6, wherein the plurality of wavelengths and the plurality of intensities of light comprise: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a fourth wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity, light having a fourth wavelength and a second intensity.
A sample analysis apparatus, comprising:

a preparation section for preparing a measurement sample required for a project by a sample and a reagent;

an illumination means for illuminating the measurement sample with light of a plurality of wavelengths and a plurality of intensities;

a light receiving unit for receiving an output optical signal of the measurement sample irradiated by the light irradiation unit to acquire optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities;

and the analysis component is used for selecting the optical information corresponding to the light with specific wavelength and intensity from the optical information corresponding to the light with multiple wavelengths and multiple intensities according to the content of the interferent in the sample to analyze the sample.
The sample analysis device of claim 8, wherein the items of the test sample are measured for light having matching dominant wavelengths and dominant intensities;

when the content of the interferent in the sample is less than a first threshold value, the analysis component selects optical information corresponding to the light with the main wavelength and the main intensity corresponding to the item of the measured sample from the optical information corresponding to the light with the multiple wavelengths and the multiple intensities to analyze the sample;

when the content of the interfering substance in the sample is larger than the first threshold, the analysis component selects optical information corresponding to light with a wavelength larger than the main wavelength corresponding to the item of the measurement sample and/or light with an intensity larger than the main intensity corresponding to the item of the measurement sample from the optical information corresponding to the light with the plurality of wavelengths and the plurality of intensities to analyze the sample.
The sample analyzer as claimed in claim 9, wherein when the content of the interfering substance in the sample is greater than a first threshold value and less than a second threshold value, the analyzing means selects optical information corresponding to light having a wavelength greater than a dominant wavelength corresponding to an item of the measurement sample and having an intensity equal to a dominant intensity corresponding to an item of the measurement sample, from among optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, and analyzes the sample;

when the content of the interfering substance in the sample is greater than the second threshold, the analyzing unit selects optical information corresponding to light having a wavelength greater than a main wavelength corresponding to an item of the measurement sample and having an intensity greater than a main intensity corresponding to an item of the measurement sample from among the optical information corresponding to the plurality of wavelengths and the plurality of intensities of light, and analyzes the sample.
The sample analyzer according to claim 9 or 10, wherein the analyzer further determines whether or not the item of the measurement sample supports measurement with light of a non-dominant wavelength when the content of the interfering substance in the sample is larger than the first threshold, and if not, the analyzer selects optical information corresponding to light having a wavelength equal to the dominant wavelength corresponding to the item of the measurement sample and having an intensity larger than the dominant intensity corresponding to the item of the measurement sample from among the optical information corresponding to the light of the plurality of wavelengths and the plurality of intensities to analyze the sample.
The sample analysis device of claim 8, wherein the plurality of wavelengths comprises: a first dominant wavelength (405 nm) corresponding to a chromophoric substrate method measured item, a second dominant wavelength (575 nm) corresponding to an immunoturbidimetric method measured item, a third dominant wavelength (660 nm) corresponding to a coagulation method measured item, and a fourth wavelength (800 nm) not less than the first dominant wavelength, the second dominant wavelength, and the third dominant wavelength.
The sample analysis device of claim 8 or 12, wherein the plurality of intensities comprises at least a first intensity and a second intensity that is greater than the first intensity.
The sample analysis device of claim 13, wherein the plurality of wavelengths and the plurality of intensities of light comprise: light having a first dominant wavelength and a first intensity, light having a second dominant wavelength and a first intensity, light having a third dominant wavelength and a first intensity, light having a fourth wavelength and a first intensity, light having a first dominant wavelength and a second intensity, light having a second dominant wavelength and a second intensity, light having a third dominant wavelength and a second intensity, light having a fourth wavelength and a second intensity.
A computer-readable storage medium, characterized by comprising a program executable by a processor to implement the method of any one of claims 1 to 7.