WO2021207898A1

WO2021207898A1 - Sample analysis device and method

Info

Publication number: WO2021207898A1
Application number: PCT/CN2020/084551
Authority: WO
Inventors: 孙骁; 郭文恒; 杨雨; 武振兴; 李鑫
Original assignee: 深圳迈瑞生物医疗电子股份有限公司; 北京深迈瑞医疗电子技术研究院有限公司
Priority date: 2020-04-13
Filing date: 2020-04-13
Publication date: 2021-10-21
Also published as: CN115280155A

Abstract

A sample analysis device and method. A light irradiation unit (50) irradiates a first container containing a test sample at a sample test location. The test sample is prepared from a sample to undergo testing and a detection reagent. The light irradiation unit (50) can provide, to the sample test location, light having a first intensity and light having a second intensity, where the first intensity is less than the second intensity.

Description

Sample analysis device and method

Technical field

The invention relates to a sample analysis device and method.

Background technique

The sample analysis device is a type of device used to analyze the biochemical characteristics of the sample. It is widely used in the clinical medical field to help medical staff diagnose the patient's condition. Take a blood coagulometer as an example. The blood coagulometer can measure the clotting time of blood and the concentration or activity of related substances; the blood coagulometer can use optical methods to detect coagulation items. Light irradiates and analyzes the scattered or transmitted light to obtain optical information such as the absorbance of the solution, so as to obtain the solidification time or the concentration of the analyte.

The optical method detects coagulation items through the optical information of the light scattering, reflection or transmission of the reaction solution. Therefore, when the sample has interfering substances that change the nature of the light scattering, reflection or transmission of the reaction solution, this will affect the measurement. , Making the detection result inaccurate, and in severe cases, the detection result may not even be obtained at all.

Summary of the invention

technical problem

The present invention mainly provides a sample analysis device and method, which will be described in detail below.

The solution to the problem

Technical solutions

According to the first aspect, an embodiment provides a sample analysis device, including:

The light component is used to illuminate the first container that is located at the sample measurement position and contains the measurement sample, the measurement sample is prepared from the sample to be tested and the detection reagent; wherein the light component can provide the first container for the sample measurement position Light of one intensity and light of a second intensity, the first intensity being less than the second intensity;

The optical detection component includes a first detector adjacent to the sample measurement position, and is configured to receive the output light signal after the illumination component irradiates the first container to obtain the optical detection information of the measurement sample. The optical detection information includes first optical detection information corresponding to light of a first intensity and second optical detection information corresponding to light of a second intensity;

The analysis component is used to select the second optical detection information corresponding to the second intensity of light to analyze the sample detection item when the interference of the sample to be tested exceeds a preset threshold. When the preset threshold is not exceeded, the first optical detection information corresponding to the light of the first intensity is selected for analysis of the sample detection item.

In an embodiment, the light-emitting component provides light of a first intensity and light of a second intensity to the sample measuring position during each light cycle.

In an embodiment, the illumination component includes a multi-wavelength light source, which sequentially outputs different illumination lights in a preset sequence during each illumination period, and the different illumination lights in each illumination period include light with a first wavelength and a first intensity. And the light of the first wavelength and the second intensity, or the light of the first wavelength and the first intensity and the light of the second wavelength and the second intensity.

In one embodiment, the light of the first intensity provided by the light-emitting component includes: light of the first wavelength for determination of the chromogenic substrate method, light of the second wavelength for determination of the immunoturbidimetric method, and light of the second wavelength for determination of the coagulation method. At least one of the light of the third wavelength; preferably, the range of the first wavelength is 340nm-420nm, the range of the second wavelength is 520nm-590nm, and the range of the third wavelength is 660nm-800nm.

In one embodiment, the illumination component sequentially outputs light of the first wavelength and the first intensity, the light of the second wavelength and the first intensity, the light of the third wavelength and the first intensity in a predetermined order in each illumination period. , The light of the fourth wavelength and the first intensity and the light of the fourth wavelength and the second intensity, wherein the first wavelength <the second wavelength <the third wavelength ≤ the fourth wavelength.

In an embodiment, the sample analysis device further includes an interference detection component, and the interference detection component includes at least one interference detection position and a second detector adjacent to the interference detection position; The component is used for irradiating a second container at the interference detection position and at least containing a sample, and the second detector is used for receiving the output light signal of the second container after being irradiated by the illuminating component, so as to obtain the to-be-tested The interference detection information of the sample; the interference detection information is used to indicate whether the interference of the sample to be tested exceeds a preset threshold.

In one embodiment, the illuminating component irradiates a first container containing a measurement sample at the sample measurement position by light of a first intensity, and the first detector is used to receive the first container being illuminated by the illuminating component. The irradiated output light signal is used to obtain interference detection information of the sample to be tested; the interference detection information is used to determine whether the interference of the sample to be tested exceeds a preset threshold.

In an embodiment, the sample analysis device further includes a dispensing mechanism and a controller, and the controller is used to control the dispensing mechanism to dispense a part of the sample to be tested and the diluent into the second container Or control the dispensing mechanism to dispense the sample to be tested and the detection reagent into the first container.

In one embodiment, the sample to be tested is blood, and the interfering substance includes at least one of hemoglobin, bilirubin, and chyle.

In an embodiment, there are multiple sample measurement positions; the illumination component includes a light source and a multi-fiber bundle, and the multi-fiber bundle includes a plurality of optical fibers corresponding to the sample measurement positions, each The optical fiber is used to provide the light of the first intensity and the light of the second intensity to the corresponding sample measurement position.

In an embodiment, the light source includes a first light source, a second light source, and a third light source, which respectively provide light of a first wavelength, a second wavelength, and a third wavelength. The first wavelength, the second wavelength, and the third wavelength The light is the first intensity.

In an embodiment, the illumination component further includes a fourth light source for providing light of the fourth wavelength and the first intensity and the light of the fourth wavelength and the second intensity in a time sharing period in one illumination period.

In an embodiment, the lighting component further includes a driving circuit connected to the first light source, the second light source, the third light source, and the fourth light source, and is configured to provide a first driving current to drive the first light source. The light source, the second light source, and the third light source generate light of the first intensity; and are also used to provide a first driving current and a second driving current to drive the fourth power source to generate the first intensity according to the first aspect, An embodiment provides a sample analysis device, including:

In an embodiment, the lighting component further includes a driving circuit connected to the first light source, the second light source, the third light source, and the fourth light source, and is configured to provide a first driving current to drive the first light source. The light source, the second light source, and the third light source generate light of the first intensity; and are also used to provide a first drive current and a second drive current to drive the fourth power source to generate the first intensity and second intensity in a time-sharing manner. Light, the second drive current is greater than the first drive current.

In an embodiment, the illumination component includes a multi-wavelength light source and a rotating filter, the rotating filter includes a filter and an attenuator, and the illumination component is used to provide time sharing when the rotating filter rotates. Different wavelengths and different intensities of light.

In an embodiment, the illumination component includes a plurality of multi-wavelength light sources, respectively corresponding to the plurality of sample measurement positions.

According to a second aspect, an embodiment provides a sample analysis device, including:

The analysis component is used to select the first optical detection information corresponding to the light of the first intensity or the second optical detection information corresponding to the light of the second intensity according to a preset condition, and analyze the sample detection result.

In an embodiment, the preset condition is a condition related to sample interference detection information, and the analysis component is configured to select a light corresponding to the second intensity when the interference of the sample to be tested exceeds a preset threshold. When the interference of the sample to be tested does not exceed the preset threshold value, the first optical detection information corresponding to the light of the first intensity is selected for analysis.

In an embodiment, the sample interference detection information includes at least one of absorbance or luminous flux of the sample to be tested.

In one embodiment, the illumination component includes a multi-wavelength light source, which sequentially outputs different illumination lights in a preset sequence during each illumination period, and the different illumination lights in each illumination period include the first wavelength and the first wavelength provided by time sharing. Light of one intensity and light of first wavelength and second intensity, or light of first wavelength and first intensity and light of second wavelength and second intensity provided by time sharing.

In an embodiment, the light of the second intensity provided by the illuminating component includes light of a fourth wavelength, and the fourth wavelength is not less than any one of the first wavelength, the second wavelength, or the third wavelength.

According to a third aspect, an embodiment provides a method of sample analysis, including:

In one light cycle, irradiate a first container with a measurement sample located at the sample measurement position with a first intensity of light and a second intensity of light; the measurement sample is prepared from the sample to be tested and the detection reagent;

Acquiring the optical detection information corresponding to the light of the first intensity and the light of the second intensity;

Obtain the interference detection information of the sample to be tested;

If the interference of the sample to be tested exceeds the preset threshold, select the optical detection information corresponding to the second intensity light;

If the interference of the sample to be tested does not exceed the preset threshold, select the optical detection information corresponding to the light of the first intensity; and

According to the selected optical inspection information, analyze the sample inspection results.

In an embodiment, the irradiating the first container with the measurement sample at the sample measurement position with the first intensity of light and the second intensity of light includes:

In one illumination period, the measurement sample of the sample measurement position is time-divisionally irradiated with the first wavelength and first intensity light, the first wavelength and the second intensity light; or within one illumination period, the sample measurement position is measured The sample is irradiated with the light of the first wavelength and the first intensity, and the light of the second wavelength and the second intensity in a time-sharing manner.

In one light cycle, the measurement sample of the sample measurement position is time-shared irradiated with light of the first wavelength and first intensity, light of the second wavelength and first intensity, light of the third wavelength and first intensity, and fourth wavelength And light of a first intensity and light of a fourth wavelength and a second intensity, wherein the first wavelength <the second wavelength <the third wavelength ≤ the fourth wavelength;

Preferably, the range of the first wavelength is 340nm-420nm, the range of the second wavelength is 520nm-590nm, and the range of the third wavelength is 660nm-800nm.

In an embodiment, the obtaining interference detection information of the sample to be tested includes dispensing a part of the sample to be tested and the diluent into the second container.

In an embodiment, the sample analysis method further includes dispensing another part of the sample to be tested and a detection reagent into the first container to prepare the measurement sample; and transporting the first container to the Sample measurement position.

In an embodiment, the obtaining interference detection information of the sample to be tested includes performing interference detection on the sample to be tested before preparing the measurement sample.

In an embodiment, the obtaining the interference detection information of the sample to be tested includes using the first intensity of light to perform interference detection on the measurement sample at the measurement position of the sample.

In an embodiment, the sample analysis method further includes outputting the sample detection result and interference detection information after analyzing the sample detection result.

In an embodiment, the interference detection information includes at least one of the absorbance of the sample to be tested to light of a predetermined wavelength or the luminous flux of the sample to be tested.

In an embodiment, the sample to be tested is blood, and the interfering substance includes at least one of hemoglobin, bilirubin, and chyle.

According to a fourth aspect, an embodiment provides a computer-readable storage medium including a program that can be executed by a processor to implement the method described in any of the embodiments herein.

The beneficial effects of the invention

Brief description of the drawings

Description of the drawings

Figure 1 is a schematic diagram of the absorption spectra of the three interfering substances, hemoglobin, bilirubin and chyle, for light in each band range;

2 is a schematic diagram of the structure of a sample analysis device according to an embodiment;

FIG. 3 is a schematic structural diagram of a sample analysis device of another embodiment;

FIG. 4 is a schematic structural diagram of a sample analysis device according to another embodiment;

Figure 5(a) is a schematic diagram of the light provided by the illumination component during the illumination period; Figure 5(b) is another schematic diagram of the light provided by the illumination component during the illumination period;

Figure 6(a) is a schematic diagram of the light provided by the illumination component during the light period; Figure 6(b) is another schematic diagram of the light provided by the illumination component during the light period;

FIG. 7 is a schematic diagram of the structure of an illumination component according to an embodiment;

FIG. 8 is a schematic diagram of the structure of a lighting component of another embodiment;

FIG. 9 is a schematic diagram of a structure of a lighting component according to another embodiment;

Figure 10 is a schematic diagram of the structure of the illumination component of another embodiment

Fig. 11 is a schematic diagram of a structure of a lighting component according to another embodiment

FIG. 12 is a schematic diagram of the structure of a lighting component according to another embodiment;

FIG. 13 is a schematic structural diagram of a sample analysis device according to another embodiment;

FIG. 14 is a schematic flowchart of a sample analysis method according to an embodiment;

FIG. 15 is a schematic flowchart of a sample analysis method according to another embodiment;

FIG. 16 is a schematic flowchart of a sample analysis method according to another embodiment;

FIG. 17 is a schematic flowchart of a sample analysis method according to another embodiment.

Invention embodiment

Embodiments of the present invention

Hereinafter, the present invention will be further described in detail through specific embodiments in conjunction with the accompanying drawings. Among them, similar elements in different embodiments use related similar element numbers. In the following embodiments, many detailed descriptions are used to make this application better understood. However, those skilled in the art can easily realize that some of the features can be omitted under different circumstances, or can be replaced by other elements, materials, and methods. In some cases, some operations related to this application are not shown or described in the specification. This is to avoid the core part of this application being overwhelmed by excessive descriptions. For those skilled in the art, these are described in detail. Related operations are not necessary, they can fully understand the related operations based on the description in the manual and the general technical knowledge in the field.

In addition, the features, operations, or features described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be sequentially exchanged or adjusted in a manner obvious to those skilled in the art. Therefore, the various sequences in the specification and the drawings are only for clearly describing a certain embodiment, and are not meant to be a necessary sequence, unless a certain sequence is required to be followed unless otherwise stated.

The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the described objects and do not have any sequence or technical meaning. The "connection" and "connection" mentioned in this application include direct and indirect connection (connection) unless otherwise specified.

In the optical determination of blood coagulation, it can generally be divided into three methods: coagulation method, immunoturbidimetric method and chromogenic substrate method. The chromogenic substrate method usually uses 340nm-420nm violet or ultraviolet light, and the chromogenic substrate method is usually used to determine detection items such as antithrombin-III (AT-III or AT3). The immunoturbidimetric method usually uses yellow-green light of 520nm-590nm, and the immunoturbidimetric method is usually used to determine detection items such as D-dimer (DD) and fibrin/fibrinogen degradation products (FDP). The coagulation method usually uses red light or infrared light at 660nm-800nm. The coagulation method is usually used to determine the coagulation time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB). ) And other test items.

Whether it is coagulation method, immunoturbidimetric method or chromogenic substrate method, in the optical determination of blood coagulation, when there are interfering substances in the sample, it will interfere with the detection; for example, when there are such as hemoglobin and bilirubin in the sample plasma In the case of interfering substances such as chyle, these interfering substances have a strong absorption of light, so they will interfere with the sample detection. Generally, hemoglobin, bilirubin, and chyle can be collectively referred to as HIL interference, where H refers to hemoglobin, I refers to bilirubin, and L refers to chyle. Please refer to Figure 1, which is a schematic diagram of the absorption spectra of the three interfering substances of hemoglobin, bilirubin and chyle to the light of each band range. Light below 600nm has strong absorption, which greatly reduces the light transmittance of the mixture of sample and detection reagent. The actual light that can be received is very small, which affects the accuracy and reliability of optical measurement; sometimes it can be received The received light is even almost zero, making it impossible to identify the reaction process between the sample and the detection reagent.

One solution to the interference is to provide light in a wavelength band that will not be absorbed by the interference in the sample for detection. For example, provide a larger wavelength like 800nm light to illuminate the mixture of the sample and the detection reagent, as can be clearly seen from the figure , Hemoglobin and bilirubin have almost no absorption of light with wavelengths greater than 800nm, and chyle has relatively small absorption of light with wavelengths greater than 800nm. This solution has some disadvantages. For example, although the absorption of light with a wavelength greater than 800 nm by chyle is relatively small, relatively speaking, the light absorption by chyle is still not negligible when the detection results are required to be more accurate; in addition, When the concentration of chyle in the sample is relatively high, even in occasions where the accuracy of the test result is generally required (such as physical examination, etc.), the absorption of light by chyle is still not negligible, and the chyle will still seriously affect the accuracy of the test result. ; Finally, providing light with a larger wavelength, such as 800nm light, is not suitable for items such as the detection of the chromogenic substrate method, because from the detection principle, the chromogenic substrate method is due to the reaction between the sample and the detection reagent Later, the detection reagent replaces the substance in the sample. The replaced substance only absorbs in the range of ultraviolet and violet light, so generally only the violet light or ultraviolet light of 340nm-420nm mentioned above can be used, and light of other wavelength bands cannot be used. The coagulation method and the immunoturbidimetric method not only use the light of the respective wavelength ranges mentioned above, but also can theoretically use the light of other wavelength ranges for detection.

The applicant has conducted research on the above-mentioned problems and proposed another solution path, which is to increase the light intensity to compensate for the influence of too low luminous flux caused by the interference, so as to solve the influence of the interference in the sample on the item detection. Specifically, when there is no interference substance in the sample or the interference substance content is low, light with normal light intensity is used for detection, and when the interference substance content in the sample is high, light with greater intensity is used for detection. The present invention will be described below.

A sample analysis device is disclosed in some embodiments of the present invention. The sample analysis device is an instrument used to analyze and measure samples. Let us take a blood coagulation analyzer (that is, the blood coagulometer mentioned in this article) as an example to illustrate the test process of the sample analysis device. The test procedure of a blood coagulation analyzer is generally as follows: complete the addition of samples, such as blood or plasma, and the addition of test reagents into a container, such as a reaction cup, to prepare a test sample (or called a mixture, a reaction solution, etc.), and then After the measurement sample is incubated, the reaction cup is placed in a preset position such as the sample measurement position. The coagulation analyzer can irradiate the measurement sample in the reaction cup with, for example, multi-wavelength light, and pass the coagulation method, immunoturbidimetric method or hair Color substrate method and other analysis to obtain the coagulation reaction curve of the measurement sample over time, so as to further calculate the coagulation time of the measurement sample or other coagulation-related performance parameters.

In the stage of measuring the measurement sample, the core component of the sample analysis device is the component that provides light, the component that receives the transmission, reflection, or scattering of light from the measurement sample, and the component that analyzes the information of the received light. Therefore, referring to FIG. 2, the sample analysis device in some embodiments may include an illumination component 50, an optical detection component 60 and an analysis component 70. 3 and 4, the sample analysis device in some embodiments may further include one or more of the sample unit 10, the reagent unit 20, the dispensing mechanism 30, and the controller 40.

The sample unit 10 is used to carry samples. In some examples, the sample unit 10 may include a sample distribution module (SDM, Sample Delivery Module) and a front-end track; in other examples, the sample unit 10 may also be a sample tray—for example, as shown in Figure 4, the sample tray includes multiple The sample position of the container can be placed, and the sample tray can be dispatched to a corresponding position by rotating its disk structure, such as a position for the dispensing mechanism 30 to aspirate the sample.

The reagent unit 20 is used to carry reagents. In one embodiment, the reagent unit 20 is arranged in a disc-shaped structure, and the reagent unit 20 has a plurality of positions for carrying reagent containers. The reagent unit 20 can rotate and drive the reagent container it carries to rotate for rotating the reagent container. Go to the reagent suction position for the dispensing mechanism 30 to suck reagents. The number of reagent units 20 may be one or more.

The dispensing mechanism 30 is used to suck samples or reagents and discharge them. In some embodiments, the dispensing mechanism 30 may include a sample dispensing mechanism 31 and a reagent dispensing mechanism 33. The sample dispensing mechanism 31 is used to suck samples and discharge them into the container to be added. In some embodiments, the sample dispensing mechanism 31 may include a sample needle, and the sample needle performs a two-dimensional or three-dimensional movement in space through a two-dimensional or three-dimensional drive mechanism, so that the sample needle can move to aspirate the sample carried by the unit 10 The sample, and move to the position of the container to be added, and discharge the sample into the container. In some embodiments, the reagent dispensing mechanism 33 may include a reagent needle, and the reagent needle performs a two-dimensional or three-dimensional movement in space through a two-dimensional or three-dimensional drive mechanism, so that the reagent needle can move to absorb the contents of the reagent unit 20. Reagent, and move to the position of the container to be added, and discharge the reagent into the container. In some embodiments, the sample dispensing mechanism 31 and the reagent dispensing mechanism 33 may also share a driving mechanism and a needle tube. When the sample dispensing mechanism 31 and the reagent dispensing mechanism 33 share a driving mechanism and needle tube, the sample and the needle tube When drawing reagents, you need to clean the needle tube to avoid problems such as cross-contamination.

The reaction part 41 in FIG. 4 is used to carry a measurement sample prepared from a sample and a detection reagent. In an example, the reaction part 41 is arranged in a disc-shaped structure and has a plurality of placement positions for placing the first container, such as a reaction cup. The reaction part 41 can rotate and drive the reaction cup in the placement position to rotate for The reaction cup is arranged in the reaction plate and the mixed solution in the incubation reaction cup is incubated. The sample detection position may be on the reaction part 41, that is, some placement positions on the reaction part 41 are sample detection positions; the sample detection position may also be set independently of the reaction part 41, that is, set at a position close to the reaction part 41, for example.

The light-emitting member 50 is used to provide light for measurement. In some embodiments, the lighting component 50 can provide light of two intensities, such as light of a first intensity and light of a second intensity, where the first intensity is less than the second intensity. The light of the first intensity can be the intensity of light normally used for measuring the sample, and the light of the second intensity is light with a stronger intensity than the light of the first intensity, and can be used to measure the sample containing interferences in the present invention. Specifically, the light component 50 is used to illuminate the first container (such as a reaction cup) that is located at the sample measurement position and contains the measurement sample—as described above, the measurement sample is prepared by the sample to be tested and the detection reagent; the light component 50 The first intensity light and the second intensity light can be provided to the sample measurement position.

In some embodiments, the light component 50 provides the first intensity light and the second intensity light to the sample measurement position during each light period. For example, please refer to FIG. 5(a). It is assumed that the first wavelength and first intensity light, the first wavelength and the second intensity light are output in sequence; or, referring to Figure 5(b), the first wavelength and the first wavelength and the second intensity are output in each light cycle according to the preset compliance First intensity light, second wavelength and second intensity light.

In order to allow the sample measurement position to be tested for most items, that is, to support detection items such as determination by coagulation, immunoturbidimetry, and chromogenic substrates, the illumination component 50 may include a multi-wavelength light source, that is, the sample measurement position can be tested. Provides light of multiple wavelengths. For example, the light of the first intensity provided by the illumination unit 50 includes: light of the first wavelength for measurement by the chromogenic substrate method, light of the second wavelength for measurement by the immunoturbidimetric method, and light of the third wavelength for measurement by the coagulation method. At least one of, that is, one, two, or three; in some embodiments, the range of the first wavelength is 340nm-420nm, the range of the second wavelength is 520nm-590nm, and the range of the third wavelength is 660nm-800nm . Further, the light of the second intensity provided by the illuminating component 50 may include light of a fourth wavelength, and the fourth wavelength is not less than any one of the first wavelength, the second wavelength, or the third wavelength; in an example, the first wavelength <Second wavelength<Third wavelength≤Fourth wavelength. In a specific example, the light component 50 can output light of the first wavelength and the first intensity, the light of the second wavelength and the first intensity, the light of the third wavelength and the first intensity in each light cycle according to the preset compliance. Light, fourth wavelength and first intensity light, and fourth wavelength and second intensity light. In another specific example, the illuminating component 50 can sequentially output light of the first wavelength and the first intensity, the light of the third wavelength and the first intensity, the second wavelength and the first intensity in each light cycle according to the preset compliance. Light, fourth wavelength and first intensity light, and fourth wavelength and second intensity light. In another specific example, the illuminating component 50 can sequentially output light of the first wavelength and the first intensity, the light of the third wavelength and the first intensity, the second wavelength and the first intensity in each light cycle according to the preset compliance. Light, fourth wavelength and first intensity light, first wavelength and second intensity light, third wavelength and second intensity light, second wavelength and second intensity light, fourth wavelength and second intensity The light. Figure 6(a) and Figure 6(b) are two examples.

In order to simplify the structure, please refer to FIG. 7. In some embodiments, the illumination component 50 may include a light source 51 and a multi-fiber bundle 59, so that the light source 51 can provide light to multiple sample measurement positions, specifically, a multi-fiber The bundle 59 includes a plurality of optical fibers respectively corresponding to the plurality of sample measurement positions, and each optical fiber is used to provide the first intensity light and the second intensity light to the corresponding sample measurement position. Specifically, referring to FIG. 8, the light source 51 may include a first light source 52, a second light source 53, and a third light source 54. The first light source 52 provides light of a first wavelength, the second light source 53 provides a second wavelength, and the third light source 54 provides the light of the third wavelength, and the light of the first wavelength, the second wavelength and the third wavelength are all of the first intensity. In some embodiments, the light source 51 may further include a fourth light source 55 for providing light of the fourth wavelength and the first intensity and the light of the fourth wavelength and the second intensity in a time sharing period; that is, the fourth light source 55 can provide two luminosity of the fourth wavelength of light. Understandably, in order to improve the light performance, some optical components for focusing, such as focusing lens, can be added between the light source 51 and the multi-fiber bundle 59; A collimating lens can also be added to improve the performance of the light directed to the measurement position of the sample.

The light source 51 provides the light of the above wavelength and light intensity through many structures, which will be described in detail below.

In some embodiments, referring to FIG. 9, the lighting component 50 further includes a driving circuit 56, which is connected to the first light source 52, the second light source 53, the third light source 54 and the fourth light source 55, and is used to provide a first light source. A driving current drives the first light source 52, the second light source 53, and the third light source 54 to generate the first intensity of light; and is also used to provide the first driving current and the second driving current to drive the fourth power source 55 to generate the first intensity in time sharing And the second intensity of light, the second driving current is greater than the first driving current. By providing different driving currents to the fourth light source 55, the fourth light source 55 generates light of different intensities. For example, in Figure 6(a) above, in one light cycle, the first light source 52, the second light source 53, the third light source 54 and the fourth light source 55 are controlled by the driving circuit 56 to blink at a time to ensure that each light is irradiated. The reaction cup is a designated wavelength; when driven, the LEDs of the first light source 52, the second light source 53, and the third light source 54 flash once each, the fourth light source 55 flashes twice, and the fourth light source 55 flashes twice during one light cycle. The lighting of the light source 55 adopts different driving currents, that is, the light source flickers five times in one cycle; the first four lighting cycles in a light cycle are used for normal testing of samples without interference, and finally a larger drive The current lighting the fourth light source 55 is used to test samples with interferences. One light cycle in this article can be 0.1s.

Similarly, in some embodiments, the driving circuit 56 may also provide different driving currents to the first light source 52, so that the first light source 52 generates light of different intensities; in some embodiments, the driving circuit 56 may also provide different driving currents to the first light source 52. The two light sources 53 provide different driving currents, so that the second light source 53 generates different intensities of light; in some embodiments, the driving circuit 56 may also provide different driving currents to the third light source 54 so that the third light source 54 generates Different intensities of light.

In some embodiments, referring to FIG. 10, the light source 51 can also be realized by a multi-wavelength light source 57 and a rotating filter 58. The multi-wavelength light source 57 provides light of multiple wavelengths, such as light of a first wavelength, light of a second wavelength, light of a third wavelength, and light of a fourth wavelength. In some examples, the multi-wavelength light source 57 may be implemented by a halogen lamp. The rotating filter 58 includes a filter and an attenuator. The light component 50 is used to provide light of different wavelengths and different intensities in time sharing when the rotating filter 58 rotates, for example, to provide light of a first wavelength and a first intensity, Light of the second wavelength and first intensity, light of the third wavelength and first intensity, light of the fourth wavelength and first intensity, and light of the fourth wavelength and second intensity. Then, through a multi-fiber bundle 59, the light can be provided to a plurality of sample measurement positions. It is understandable that some optical components, such as lens groups, can be added between the multi-wavelength light source 57 and the rotating filter 58 to improve the performance of the illuminating light.

The above is an example in which the illuminating unit 50 provides light of different wavelengths and different intensities to a plurality of samples when measuring positions of a plurality of samples through a multi-fiber bundle 59.

In some examples, the illumination component 50 may also be equipped with a multi-wavelength light source for each sample measurement position, and each multi-wavelength light source can provide light of different wavelengths and different intensities to the corresponding sample measurement position.

Referring to FIG. 11, in some examples, the illumination component 50 may also be equipped with a single-wavelength light source for each sample measurement position, and each single-wavelength light source can provide light of a single wavelength and different intensities to the corresponding sample measurement position. For example, some sample measurement positions are equipped with a light source of the first wavelength, which can provide light of the first wavelength and first intensity, and light of the first wavelength and second intensity, and light of different intensities to the corresponding sample measurement position. It can be achieved by different drive currents or attenuating sheets. In these sample measurement positions, the test items supporting the chromogenic substrate method can be measured; similarly, some sample measurement positions are equipped with a second wavelength light source. The light source can provide the light of the second wavelength and the first intensity, and the light of the second wavelength and the second intensity to the corresponding sample measurement position. In these sample measurement positions, the test items that support the immunoturbidimetric method can be measured; some sample measurement The light source of the third wavelength is configured in the position, and the light source of the third wavelength can provide the light of the third wavelength and the first intensity, and the light of the third wavelength and the second intensity to the corresponding sample measurement position. Determine the test items that support the coagulation method.

In some cases, the sample analysis device also provides an interference detection position, which will be further mentioned below. The illumination component 50 can also provide illumination to the interference detection position, for example, through a multi-fiber bundle 59, so that the illumination component 50 irradiates the same light to the sample detection position and the interference detection position. FIG. 12 is an example. In the picture, the purple LED provides 405nm light, the green LED provides 575nm light, the red LED provides 660nm light, and the infrared LED provides 800nm light. They are combined by three dichroic mirrors and are coupled to a multi-fiber bundle. Among the optical fibers of 59, a multi-fiber bundle 59 is divided into a plurality of small ends of optical fibers, one of which can be used to illuminate the second container (such as a cuvette or reaction cup) on the interference detection position to detect interference in the sample. For example, the type and content of interferences. The small ends of the remaining optical fibers are used to illuminate the first container (such as the reaction cup) of the measurement sample. Of course, the LED light source in the picture can also be replaced with other types of light sources, such as LD light sources or halogens. Lights etc. In some examples, the light component 50 can also independently provide light to the sample detection position and the interference detection.

The above are some descriptions of the light-emitting component 50, and the optical detection component 60 will be described below.

The optical detection component 60 cooperates with the illuminating component 50. In some embodiments, the optical detection component 60 includes a first detector 61 adjacent to the sample measurement position. The first detector 61 is used to receive the output light signal of the light component 50 after irradiating the first container to obtain the optical detection information of the measurement sample. The optical detection information includes the first optical signal corresponding to the light of the first intensity. The detection information and the second optical detection information corresponding to the light of the second intensity. That is, after the illuminating member 50 irradiates the first container containing the measurement sample at the sample measurement position by the light of the first intensity, the first detector 61 senses the effect of the first container containing the measurement sample on the first container of the first intensity. Light transmission, reflection, or scattered light, etc., to obtain the first optical detection information corresponding to the light of the first intensity; similarly, the light component 50 irradiates the light of the second intensity at the measurement position of the sample and contains the measurement sample. After the first container, the first detector 61 senses the transmission, reflection, or scattered light of the second intensity of light by the first container containing the measurement sample to obtain the second optical corresponding to the second intensity of light. Detection information. In some examples, the first detector 61 detects the transmitted light that passes through the measurement sample. In some examples, the first detector 61 may be implemented by a component capable of converting an optical signal into an electrical signal, such as a photodetector, etc., specifically, it may be a photodiode PD, a photomultiplier tube PMT, an avalanche photodiode APD, Charge coupled device CCD, complementary metal oxide semiconductor CMOS, image intensified detector ICCD or electron multiplying EMCCD, etc. It is understandable that a first detector 61 is generally provided for a sample determination position, that is, each sample determination position has a corresponding first detector 61.

The analysis component 70 analyzes the sample detection items using one of the first optical detection information and the second optical detection information. In some embodiments, the analysis component 70 selects the first optical detection information corresponding to the light of the first intensity or the second optical detection information corresponding to the light of the second intensity according to a preset condition, and analyzes the sample detection result. The preset condition may be a condition related to the sample interference detection information, and the analysis component 70 is used to select the second optical detection information corresponding to the second intensity of light for analysis when the interference of the sample to be tested exceeds the preset threshold. When the interference of the sample to be tested does not exceed a preset threshold, the first optical detection information corresponding to the light of the first intensity is selected for analysis. In some embodiments, the sample interference detection information includes at least one of the absorbance or luminous flux of the sample to be tested; the absorbance of the sample to be tested represents the degree of light absorption by the sample to be tested when the sample is illuminated with light; The absorbance of the test sample at the preset wavelength exceeds the preset absorbance threshold. For example, the absorbance of at least one of 405nm, 575nm, 660nm, and 800nm exceeds the corresponding absorbance threshold, which means that the interference of the test sample exceeds the preset absorbance threshold. To set the threshold, it is necessary to increase the light intensity or other methods for anti-interference detection; the luminous flux of the sample to be tested represents the degree to which the light can pass through the sample to be tested when the sample is illuminated with light, and the luminous flux of the sample to be tested can be the sample to be tested The initial luminous flux detected before the detection of the formal coagulation item; if the initial luminous flux of the sample to be tested is lower than the preset luminous flux threshold, it means that the interference of the sample to be tested exceeds the preset threshold, and it is necessary to increase the luminous intensity. Method for anti-interference detection.

The above are some descriptions of the sample analysis device. It can be seen that there are two links involved here, one is to detect interferences on the sample to be tested, and the other is to select the optical detection information corresponding to the light of an appropriate intensity from the two light intensities to analyze the sample detection items. Described below separately.

Referring to FIG. 13, in some embodiments, the sample analysis device may further include an interference detection component 80, which includes at least one interference detection position and a second detector 81 adjacent to the interference detection position. The second detector 81 may be realized by a component capable of converting an optical signal into an electrical signal, such as a photodetector, etc., specifically, it may be a photodiode PD, a photomultiplier tube PMT, an avalanche photodiode APD, or a charge coupled device CCD , Complementary metal oxide semiconductor CMOS, image intensified detector ICCD or electron multiplying EMCCD, etc. The light component 50 is used to illuminate a second container (such as a reaction cup or a colorimetric cell, etc.) that is located at the interference detection position and at least contains a sample—for example, the light component 50 is irradiated by light of the first intensity; the second detector 81 is used After receiving the output light signal of the second container irradiated by the light component 50, the interference detection information of the sample to be tested is obtained; the interference detection information is used to indicate whether the interference of the sample to be tested exceeds a preset threshold. Therefore, from the perspective of the test process, the controller 40 can be used to control the dispensing mechanism 30 to dispense a part of the sample to be tested and the diluent into the second container, so as to perform the interference detection of the sample at the interference detection position, and then control The device can control the dispensing mechanism 30 to dispense the remaining part of the sample to be tested and the detection reagent into the first container, so as to analyze the sample detection items at the sample detection position; then, when the sample detection position, the light component 50 can sequentially send the sample to the sample The detection position provides light of the first intensity and light of the second intensity. It can also provide only one intensity of light according to the interference detection information, for example, when the interference of the sample is detected at the interference detection position and does not exceed the preset threshold , Then when the sample is detected at the sample detection position, only the first intensity of light can be provided; when the interference of the sample is detected at the interference detection position to exceed the preset threshold, the sample is then detected at the sample detection position. During detection, only the second intensity of light can be provided.

The introduction of the interference detection component 80 can detect the interferences of the sample to be tested, and obtain the detection information of the sample interferences. In some other embodiments, the interference detection position may not be provided separately, but the interference detection of the sample to be tested is realized while the sample is measured at the sample measurement position, which will be described in detail below.

In some embodiments, the light component 50 irradiates the first container containing the measurement sample at the sample measurement position by light of the first intensity, and the first detector 61 is used to receive the output light signal of the first container after being irradiated by the light component 50 , To obtain the interference detection information of the sample to be tested; the interference detection information is used to determine whether the interference of the sample to be tested exceeds a preset threshold; specifically, if the analysis component 50 passes through the first intensity light According to the corresponding first optical detection information, if it is determined that the interference of the sample to be tested does not exceed the preset threshold, the first optical detection information is directly used for the analysis of the sample detection item, and the subsequent illumination component 50 may not use the second intensity. Light to continue to illuminate the first container containing the measurement sample at the sample measurement position; on the contrary, if the analysis component 50 passes the first optical detection information corresponding to the light of the first intensity, it is determined that the interference of the sample to be tested exceeds the expected If the threshold is set, the illuminating unit 50 then uses the second intensity of light to continue to illuminate the first container containing the measurement sample at the sample measurement position, and selects the second optical detection information corresponding to the second intensity of light for sample detection Analysis of the project. Of course, the light component 50 can also provide the first intensity light and the second intensity light to the sample measurement position in one light cycle, and obtain the first optical detection information corresponding to the first intensity light and the second intensity light. According to the second optical detection information corresponding to the light, the first optical detection information is used to determine whether the interference of the sample to be tested exceeds the preset threshold, and then one of the first optical detection information and the second optical detection information is selected according to the judgment result. Carry out the analysis of sample test items. Therefore, from the perspective of the test process, the controller 40 does not need to control the dispensing mechanism 30 to dispense a part of the sample to be tested and the diluent into the second container to perform the interference detection of the sample at the interference detection position, but can directly control The dispensing mechanism 30 dispenses the sample to be tested and the detection reagent into the first container, so as to perform interference detection and sample detection item analysis at the sample detection position.

Of course, in other embodiments, the average luminous flux during the period after the first container containing the measurement sample is placed at the sample measurement position and before the start of the test can also be used to obtain interference detection information. In an example, when the sample is added to the last step, the reagent is triggered to start timing. Generally, the mixture can be mixed and moved to the sample measurement position within 3 seconds, and then the detection will start at the 10th second, between the 3rd and the 10th. In the 7s, the illumination component 50 irradiates the first container with the measurement sample at the sample measurement position through the first intensity of light, and the first detector 61 is used to receive the output light signal of the first container after being irradiated by the illumination component 50—— For example, the average luminous flux during this period of time has the lowest light transmittance to obtain the interference detection information of the sample to be tested. If the analysis component 50 judges that the interference of the sample to be tested does not exceed the preset threshold value based on the interference detection information, then the illumination component 50 still uses the first intensity of light to irradiate the measurement sample, and the analysis component 50 passes through the first intensity of light. The corresponding first optical detection information is used to analyze the sample detection items; if the analysis component 50 determines that the interference of the sample to be tested exceeds the preset threshold based on the interference detection information, the illumination component 50 next uses the second intensity of light to irradiate the measurement test. In this way, the analysis component 50 analyzes the sample detection items through the second optical detection information corresponding to the second intensity of light.

The above are some examples of sample interference detection. It is understandable that those skilled in the art can also use other methods to detect sample interferences, for example, by taking a picture of the sample to be tested, obtaining an image of the sample to be tested, and then using Methods such as machine learning are used to analyze the image to obtain the interference detection information of the sample to be tested.

The above is the sample analysis device of some embodiments of the present invention.

14 and 15, some embodiments of the present invention also provide a sample analysis method, including the following steps:

Step 110: In one light cycle, irradiate a first container at a sample measurement position and contain a measurement sample with a first intensity of light and a second intensity of light; the measurement sample is prepared from the sample to be tested and the detection reagent.

In some embodiments, in step 110, the light of the first wavelength and the first intensity, the light of the first wavelength and the second intensity are sequentially output according to a preset conformity in one light period; or, in each light period The light of the first wavelength and the first intensity, the light of the second wavelength and the second intensity are sequentially output according to the preset compliance.

In order to enable the sample measurement position to perform detection of most items, that is, to support detection items such as measurement by coagulation method, immunoturbidimetry, and chromogenic substrate, step 110 may provide light of multiple wavelengths to the sample measurement position. For example, the light of the first intensity provided in step 110 includes the light of the first wavelength used for the determination of the chromogenic substrate method, the light of the second wavelength used for the measurement of the immunoturbidimetric method, and the light of the third wavelength used for the measurement of the coagulation method. In some embodiments, the first wavelength ranges from 340 nm to 420 nm, the second wavelength ranges from 520 nm to 590 nm, and the third wavelength ranges from 660 nm to 800 nm. Further, the light of the second intensity provided in step 110 may include light of a fourth wavelength, and the fourth wavelength is not less than any one of the first wavelength, the second wavelength, or the third wavelength; in an example, the first wavelength < The second wavelength <the third wavelength ≤ the fourth wavelength. In a specific example, step 110 may sequentially output light of the first wavelength and the first intensity, the light of the second wavelength and the first intensity, the light of the third wavelength and the first intensity in each light cycle according to the preset compliance. , The light of the fourth wavelength and the first intensity and the light of the fourth wavelength and the second intensity. In another specific example, step 110 may sequentially output light of the first wavelength and the first intensity, the light of the third wavelength and the first intensity, the light of the second wavelength and the first intensity in each light cycle according to the preset compliance. Light, fourth wavelength and first intensity light and fourth wavelength and second intensity light. In another specific example, step 110 may sequentially output light of the first wavelength and the first intensity, the light of the third wavelength and the first intensity, the light of the second wavelength and the first intensity in each light cycle according to the preset compliance. Light, fourth wavelength and first intensity light, first wavelength and second intensity light, third wavelength and second intensity light, second wavelength and second intensity light, fourth wavelength and second intensity light Light.

Step 120: Obtain the optical detection information corresponding to the light of the first intensity and the light of the second intensity.

For example, in step 110, after irradiating the first container at the sample measurement position and containing the measurement sample by light of the first intensity, step 120 detects the transmission and reflection of light of the first intensity by the first container containing the measurement sample. Or scattered light, etc., to obtain the first optical detection information corresponding to the light of the first intensity; similarly, in step 110, the first container at the sample measurement position and containing the measurement sample is irradiated with the light of the second intensity, and then the step 120 obtains the second optical detection information corresponding to the light of the second intensity by sensing the transmission, reflection, or scattering of the light of the second intensity by the first container containing the measurement sample. In some examples, step 120 detects the light transmitted through the measurement sample.

Step 130: Obtain interference detection information of the sample to be tested.

In some embodiments, the interference detection information includes at least one of the absorbance of the sample to be tested to light of a predetermined wavelength or the luminous flux of the sample to be tested. The absorbance of the sample to be tested represents the degree of absorption of light by the sample to be tested when the sample is irradiated with light; if the absorbance of the sample to be tested to the preset wavelength exceeds the preset absorbance threshold, for example, for 405nm, 575nm, 660nm, The absorbance of at least one of 800nm exceeds the corresponding absorbance threshold, which means that the interference of the sample to be tested exceeds the preset threshold, and anti-interference detection needs to be carried out by increasing the light intensity or other methods; the luminous flux of the sample to be tested represents When the sample to be tested is irradiated with light, the degree to which light can pass through the sample to be tested. The luminous flux of the sample to be tested can be the initial luminous flux detected before the formal coagulation test of the sample to be tested; if the initial luminous flux of the sample to be tested is lower than expected The set luminous flux threshold indicates that the interference of the sample to be tested exceeds the preset threshold, and the anti-interference detection needs to be performed by increasing the light intensity.

Step 140: If the interference of the sample to be tested exceeds the preset threshold, select the optical detection information corresponding to the light of the second intensity.

Step 150: If the interference of the sample to be tested does not exceed the preset threshold, select the optical detection information corresponding to the light of the first intensity.

Step 160: According to the selected optical detection information, perform sample detection result analysis.

Step 170: Output the sample detection result and interference detection information.

The above is a flowchart of some steps of the sample analysis method. The following describes how to obtain the interference detection information of the sample to be tested in step 130.

In some embodiments, an interference detection position that is different from the measurement position of the sample may be introduced to perform interference detection on the sample, so as to detect interference information of the sample to be tested. The specific process may be to dispense a part of the sample to be tested and the diluent into the second container; in some cases, the second container may be a colorimetric cell. Correspondingly, then another part of the sample to be tested and the detection reagent can be dispensed into the first container to prepare the measurement sample, and then the first container can be transported to the sample measurement position. In this method, the interference substance detection is performed on the sample to be tested before the test sample is prepared. As for how to perform interference detection on the sample at the interference detection position, it has been described in detail above, and will not be repeated here.

In other embodiments, the sample may be detected at the sample measurement position to obtain interference detection information of the sample to be tested. For example, the first intensity light may be used to perform interference detection on the measurement sample at the sample measurement position. .

Specifically, the first optical detection information corresponding to the first intensity of light is used to obtain the interference detection information of the sample to be tested. When it is determined that the interference of the sample to be tested does not exceed a preset threshold, the first optical detection information is directly used. The detection information is used for the analysis of the sample detection items, and the light of the second intensity can be no longer used to continue to irradiate the first container with the measurement sample in the sample measurement position; on the contrary, if it passes through the light corresponding to the first intensity According to the first optical detection information, it is determined that the interference of the sample to be tested exceeds the preset threshold, and then the second intensity of light is used to continue to illuminate the first container with the measurement sample in the sample measurement position, and select the second The second optical detection information corresponding to the intensity of the light performs the analysis of the sample detection item. Of course, it is also possible to sequentially provide light of the first intensity and light of the second intensity to the sample measurement position in one light cycle, and obtain the first optical detection information corresponding to the light of the first intensity and the light corresponding to the light of the second intensity. Second optical detection information, and then use the first optical detection information as interference detection information to determine whether the interference of the sample to be tested exceeds a preset threshold, and then select the first optical detection information and the second optical detection information from the first optical detection information and the second optical detection information according to the judgment result Choose one to analyze the sample test items. Therefore, from the perspective of the test process, it is not necessary to control the dispensing mechanism 30 to dispense a part of the sample to be tested and the diluent into the second container to perform the interference detection of the sample at the interference detection position. Instead, the sample to be tested can be directly And the detection reagents are dispensed into the first container, so that the interference detection and the analysis of the sample detection items can be performed at the sample detection position.

Referring to FIG. 16, the sample analysis method of some embodiments includes the following steps:

Step 210: Obtain interference detection information of the sample to be tested.

Step 210 is to obtain the interference detection information of the sample to be tested. You can refer to the above description of obtaining the interference detection information of the sample to be tested in step 130. The interference detection can also be performed at the sample measurement position. For example, the initial luminous flux detected at the sample measurement position before the test sample is obtained for the formal coagulation item detection is used to obtain interference detection information.

Step 220: According to the acquired interference detection information of the sample to be tested, determine whether the interference of the sample to be tested exceeds a preset threshold.

Step 230: If the interference of the sample to be tested does not exceed the preset threshold, drive the lighting component with the first driving current. For example, a driving circuit is used to drive the light-emitting components, such as the first light source, the second light source, the third light source, and even the fourth light source, with the first driving current, so as to provide the first intensity light to the sample measurement position.

Step 240: If the interference of the sample to be tested exceeds the preset threshold, drive the lighting component with the second driving current. For example, the driving circuit uses the second driving current to drive the lighting component-like the fourth light source, and even the first light source, the second light source and the third light source are added to provide the second intensity of light to the sample measurement position. Understandably, the second driving current is greater than the first driving current, so that the second intensity is greater than the first intensity.

Step 250: Analyze the sample detection result according to the optical detection information. Understandably, if the first driving current is used for driving in step 230, then the optical detection information corresponding to the light of the first intensity can be obtained, and the optical detection information corresponding to the light of the first intensity can be used in step 250. Analysis of the sample detection results; if the second driving current is used for driving in step 230, the optical detection information corresponding to the second intensity of light can be obtained, and the optical detection information corresponding to the second intensity of light can be obtained in step 250. Perform sample test results analysis.

Of course, after analyzing the sample detection result in step 250, the detection result can also be output, and even the interference detection information of the sample to be tested can also be output.

Please refer to FIG. 17, the sample analysis method of some embodiments includes the following steps:

Step 310: Obtain the luminous flux of the sample to be tested.

In some examples, the luminous flux of the sample to be tested can be obtained at a detection position that is different from the sample measurement position. The specific process can be to dispense a part of the sample to be tested and the diluent to the second container; transport the second container to the above-mentioned detection position, and then illuminate the detection position through, for example, a light component—for example, the first intensity can be provided And obtain the luminous flux of the second container after being irradiated by the illuminating component; at the same time, another part of the sample to be tested and the detection reagent can be dispensed into the first container to prepare a test sample, and then the second container can be The first container is transported to the sample measurement position.

In other examples, the luminous flux of the sample to be tested can also be the initial luminous flux detected before the sample to be tested is tested for the formal coagulation item, for example, after the test sample is prepared and the test sample is started to perform the formal coagulation item at the sample measurement position Before the detection, the measurement sample is illuminated-the first intensity of light can be provided to the detection position through the illumination component to illuminate the image, and then the luminous flux after the light passes through the measurement sample is obtained.

Step 320: Determine whether the luminous flux of the sample to be tested exceeds a threshold.

Step 330: If the luminous flux of the sample to be tested exceeds the above-mentioned threshold, the light-emitting component is driven by the first driving current. For example, a driving circuit is used to drive the light-emitting components, such as the first light source, the second light source, the third light source, and even the fourth light source, with the first driving current, so as to provide the first intensity light to the sample measurement position.

Step 340: If the luminous flux of the sample to be tested does not exceed the above-mentioned threshold, drive the illumination component with the second driving current. For example, the driving circuit uses the second driving current to drive the lighting component-like the fourth light source, and even the first light source, the second light source and the third light source are added to provide the second intensity of light to the sample measurement position. Understandably, the second driving current is greater than the first driving current, so that the second intensity is greater than the first intensity.

Step 350: Analyze the sample detection result according to the optical detection information. It is understandable that if the first driving current is used for driving in step 330, then the optical detection information corresponding to the light of the first intensity can be obtained, and the optical detection information corresponding to the light of the first intensity can be used in step 350. Analysis of sample detection results; if the second driving current is used for driving in step 330, the optical detection information corresponding to the second intensity of light can be obtained, and the optical detection information corresponding to the second intensity of light can be obtained in step 350. Perform sample test results analysis.

Of course, after analyzing the sample detection result in step 350, the detection result can also be output, and even the luminous flux data of the sample to be tested can also be output.

The sample to be tested herein may be blood, and the interfering substance includes at least one of hemoglobin, bilirubin, and chyle.

In this article, stronger light—in some cases, even the wavelength of the strong light is larger, to test samples with interferences, which can effectively reduce the influence of interferences on the sample determination; but there is no interference in the normal measurement. Or for samples with less interference content, the normal light intensity is still used. This is because if a sample with no interference or less interference content is measured with stronger light, the received light detection information is If it is oversaturated, the measurement cannot be performed normally. In the specific implementation, it is possible to add a high intensity light only in one illumination period, so that the sample determination position can complete the test of samples with interferences and samples without interferences. Specifically, according to the interferences The detection result is used to select which intensity of light corresponds to the optical detection information for sample analysis, which effectively solves the influence of interference on the test and also simplifies the test process.

In summary, the present invention can adjust the light intensity when measuring the sample according to the interference detection information. When it is determined that the interference object of the sample to be tested does not exceed the preset threshold, the light intensity is adjusted, and the light of the first intensity is used to illuminate the measurement. The sample is used to perform item detection on the sample. When it is determined that the interference of the sample to be tested exceeds the preset threshold, the light intensity is adjusted, and the measurement sample is irradiated with the second intensity of light to perform the item detection on the sample. Alternatively, in the present invention, the light of the first intensity and the light of the second intensity can be used to irradiate the measurement sample in sequence within one light cycle, and then the optical corresponding to the light of one of the intensity can be selected according to the interference detection information of the sample. The detection information is used to analyze the sample detection items.

In other embodiments, during normal testing, the illumination component 50 provides the first intensity of light to test the sample. When the analysis component 70 or the user determines that re-examination is required, that is, when the sample is re-examined, the illumination component 50 provides a second intensity of light to test the sample.

This document is described with reference to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications can be made to the exemplary embodiments without departing from the scope of this document. For example, various operation steps and components used to perform the operation steps can be implemented in different ways according to a specific application or considering any number of cost functions associated with the operation of the system (for example, one or more steps can be deleted, Modify or incorporate into other steps).

In the above embodiments, it may be implemented in whole or in part by software, hardware, firmware, or any combination thereof. In addition, as understood by those skilled in the art, the principles herein can be reflected in a computer program product on a computer-readable storage medium, which is pre-installed with computer-readable program code. Any tangible, non-transitory computer-readable storage medium can be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD to ROM, DVD, Blu Ray disks, etc.), flash memory and/or the like . These computer program instructions can be loaded on a general-purpose computer, a special-purpose computer, or other programmable data processing equipment to form a machine, so that these instructions executed on the computer or other programmable data processing device can generate a device that realizes the specified function. These computer program instructions can also be stored in a computer-readable memory, which can instruct a computer or other programmable data processing equipment to operate in a specific manner, so that the instructions stored in the computer-readable memory can form a piece of Manufactured products, including realizing devices that realize designated functions. Computer program instructions can also be loaded on a computer or other programmable data processing equipment, thereby executing a series of operation steps on the computer or other programmable equipment to produce a computer-implemented process, so that the execution of the computer or other programmable equipment Instructions can provide steps for implementing specified functions.

Although the principles herein have been shown in various embodiments, many modifications to the structure, arrangement, proportions, elements, materials, and components that are particularly suitable for specific environments and operating requirements can be made without departing from the principles and scope of this disclosure. use. The above modifications and other changes or amendments will be included in the scope of this article.

The foregoing detailed description has been described with reference to various embodiments. However, those skilled in the art will recognize that various modifications and changes can be made without departing from the scope of this disclosure. Therefore, the consideration of this disclosure will be in an illustrative rather than restrictive sense, and all these modifications will be included in its scope. Likewise, the advantages, other advantages, and solutions to problems of the various embodiments have been described above. However, benefits, advantages, solutions to problems, and any elements that can produce these, or make them more specific, should not be construed as critical, necessary, or necessary. The term "including" and any other variants used in this article are non-exclusive inclusions. Such a process, method, article or device that includes a list of elements not only includes these elements, but also includes those that are not explicitly listed or are not part of the process. , Methods, systems, articles or other elements of equipment. In addition, the term "coupled" and any other variations thereof used herein refer to physical connection, electrical connection, magnetic connection, optical connection, communication connection, functional connection and/or any other connection.

Those skilled in the art will recognize that many changes can be made to the details of the above-described embodiments without departing from the basic principles of the present invention. Therefore, the scope of the present invention should only be determined by the claims.

Claims

A sample analysis device, characterized in that it comprises:

The light component is used to illuminate the first container that is located at the sample measurement position and contains the measurement sample, the measurement sample is prepared from the sample to be tested and the detection reagent; wherein the light component can provide the first container for the sample measurement position Light of one intensity and light of a second intensity, the first intensity being less than the second intensity;

The optical detection component includes a first detector adjacent to the sample measurement position, and is configured to receive the output light signal after the illumination component irradiates the first container to obtain the optical detection information of the measurement sample. The optical detection information includes first optical detection information corresponding to light of a first intensity and second optical detection information corresponding to light of a second intensity;

The analysis component is used to select the second optical detection information corresponding to the second intensity of light to analyze the sample detection item when the interference of the sample to be tested exceeds a preset threshold. When the preset threshold is not exceeded, the first optical detection information corresponding to the light of the first intensity is selected for analysis of the sample detection item.
The sample analysis device according to claim 1, wherein the light-emitting component provides the first intensity light and the second intensity light to the sample measurement position during each light period.
The sample analysis device according to claim 2, wherein the illuminating component comprises a multi-wavelength light source, and different illuminating lights are sequentially output in a preset order in each illuminating period, and different illuminating lights are output in each illuminating period. It includes light with a first wavelength and a first intensity and light with a first wavelength and a second intensity, or includes light with a first wavelength and a first intensity and light with a second wavelength and a second intensity.
The sample analysis device according to any one of claims 1 to 3, wherein the light of the first intensity provided by the illuminating component comprises: light of the first wavelength used for measurement by the chromogenic substrate method, and immunological ratio At least one of the light of the second wavelength for turbidimetric measurement and the light of the third wavelength for coagulation measurement; preferably, the range of the first wavelength is 340nm-420nm, and the range of the second wavelength is 520nm- The range of 590nm and the third wavelength is 6 60nm-800nm.
The sample analysis device according to claim 1, 2 or 4, wherein the light component outputs light of the first wavelength and the first intensity, the second wavelength and the first light in a predetermined order in each light period. Light of one intensity, light of the third wavelength and first intensity, light of the fourth wavelength and first intensity, and light of the fourth wavelength and second intensity, wherein the first wavelength <the second wavelength <the third wavelength ≤ the first wavelength Four wavelengths.
The sample analysis device according to any one of claims 1-5, further comprising an interference detection component, the interference detection component includes at least one interference detection position and a phase with the interference detection position. Adjacent second detector; the illumination component is used to illuminate a second container that is located at the interference detection position and at least contains a sample, and the second detector is used to receive the second container after being irradiated by the illumination component The optical signal is output to obtain interference detection information of the sample to be tested; the interference detection information is used to indicate whether the interference of the sample to be tested exceeds a preset threshold.
The sample analysis device according to any one of claims 1 to 5, wherein the illuminating member irradiates a first container containing a measurement sample at the sample measurement position by light of a first intensity, and The first detector is used to receive the output light signal of the first container after being irradiated by the light component to obtain interference detection information of the sample to be tested; the interference detection information is used to determine the sample to be tested Whether the interferer exceeds the preset threshold.
The sample analysis device according to claim 6 or 7, further comprising a dispensing mechanism and a controller, and the controller is used to control the dispensing mechanism to dispense a part of the sample to be tested and the diluent To the second container or control the dispensing mechanism to dispense the sample to be tested and the detection reagent into the first container.
The sample analysis device according to any one of claims 1-8, wherein the sample to be tested is blood, and the interfering substance includes at least one of hemoglobin, bilirubin, and chyle.
The sample analysis device according to any one of claims 1-9, wherein there are multiple sample measurement positions; the illumination component includes a light source and a multi-fiber bundle, and the multi-fiber bundle includes A plurality of optical fibers respectively corresponding to the sample measurement position, and each optical fiber is used to provide the first intensity light and the second intensity light to the corresponding sample measurement position.
The sample analysis device of claim 10, wherein the light source includes a first light source, a second light source, and a third light source, which provide light of a first wavelength, a second wavelength, and a third wavelength, respectively, and the first The light of the first wavelength, the second wavelength, and the third wavelength are all of the first intensity.
The sample analysis device according to claim 11, wherein the illumination component further comprises a fourth light source for providing the fourth wavelength and the first intensity of light and the fourth wavelength and The second intensity of light.
The sample analysis device of claim 12, wherein the illumination component further comprises a driving circuit, and the driving circuit is connected to the first light source, the second light source, the third light source, and the fourth light source for Provides a first drive current to drive the first light source, second light source, and third light source to generate the first intensity light; also used to provide the first drive current and the second drive current to drive the fourth power source in a time-sharing manner For the light of the first intensity and the second intensity, the second driving current is greater than the first driving current.
The sample analysis device according to claim 10, wherein the illuminating component includes a multi-wavelength light source and a rotating filter, the rotating filter includes a filter and an attenuator, and the illuminating component is used in When the rotating filter rotates, it provides light of different wavelengths and different intensities in time sharing.
9. The sample analysis device according to any one of claims 1 to 9, wherein the illumination component comprises a plurality of multi-wavelength light sources, respectively corresponding to the plurality of sample measurement positions.
A sample analysis device, characterized in that it comprises:

The light component is used to illuminate the first container that is located at the sample measurement position and contains the measurement sample, the measurement sample is prepared from the sample to be tested and the detection reagent; wherein the light component can provide the first container for the sample measurement position Light of one intensity and light of a second intensity, the first intensity being less than the second intensity;

The optical detection component includes a first detector adjacent to the sample measurement position, and is configured to receive the output light signal after the illumination component irradiates the first container to obtain the optical detection information of the measurement sample. The optical detection information includes first optical detection information corresponding to light of a first intensity and second optical detection information corresponding to light of a second intensity;

The analysis component is used to select the first optical detection information corresponding to the light of the first intensity or the second optical detection information corresponding to the light of the second intensity according to a preset condition, and analyze the sample detection result.
The sample analysis device according to claim 16, wherein the preset condition is a condition related to sample interference detection information, and the analysis component is used to detect when the interference in the sample to be tested exceeds a preset threshold. When the second optical detection information corresponding to the light of the second intensity is selected for analysis; when the interference of the sample to be tested does not exceed the preset threshold, the first optical detection information corresponding to the light of the first intensity is selected for analysis. analyze.
The sample analysis device of claim 17, wherein the sample interference detection information includes at least one of absorbance or luminous flux of the sample to be tested.
The sample analysis device according to any one of claims 16-18, wherein the illuminating component comprises a multi-wavelength light source, and different illuminating lights are sequentially outputted in a preset order in each illuminating period, and each illuminating light The different illumination light in the period includes the light of the first wavelength and the first intensity and the light of the first wavelength and the second intensity provided by time sharing, or the light of the first wavelength and the first intensity and the second wavelength provided by the time sharing And second intensity light.
The sample analysis device according to any one of claims 16-19, wherein the light of the first intensity provided by the illuminating component comprises: light of the first wavelength used for measurement by the chromogenic substrate method, and the immunological ratio At least one of the light of the second wavelength for turbidimetric measurement and the light of the third wavelength for coagulation measurement; preferably, the range of the first wavelength is 340nm-420nm, and the range of the second wavelength is 520nm- The range of 590nm and the third wavelength is 660nm-800nm.
The sample analysis device according to claim 20, wherein the light of the second intensity provided by the illuminating component includes light of a fourth wavelength, and the fourth wavelength is not less than the first wavelength, the second wavelength, or the Any one of the third wavelengths.
A method of sample analysis, characterized in that it comprises:

In one light cycle, irradiate a first container with a measurement sample located at the sample measurement position with a first intensity of light and a second intensity of light; the measurement sample is prepared from the sample to be tested and the detection reagent;

Acquiring the optical detection information corresponding to the light of the first intensity and the light of the second intensity;

Obtain the interference detection information of the sample to be tested;

If the interference of the sample to be tested exceeds the preset threshold, select the optical detection information corresponding to the second intensity light;

If the interference of the sample to be tested does not exceed the preset threshold, select the optical detection information corresponding to the light of the first intensity; and

According to the selected optical inspection information, analyze the sample inspection results.
22. The method according to claim 22, wherein the irradiating the first container with the measurement sample at the sample measurement position with the first intensity of light and the second intensity of light comprises:

In one illumination period, the measurement sample of the sample measurement position is time-divisionally irradiated with the first wavelength and first intensity light, the first wavelength and the second intensity light; or within one illumination period, the sample measurement position is measured The sample is irradiated with the light of the first wavelength and the first intensity, and the light of the second wavelength and the second intensity in a time-sharing manner.
22. The method according to claim 23, wherein the irradiating the first container with the measurement sample at the sample measurement position with the first intensity of light and the second intensity of light comprises:

In one light cycle, the measurement sample of the sample measurement position is time-shared irradiated with light of the first wavelength and first intensity, light of the second wavelength and first intensity, light of the third wavelength and first intensity, and fourth wavelength And light of a first intensity and light of a fourth wavelength and a second intensity, wherein the first wavelength <the second wavelength <the third wavelength ≤ the fourth wavelength;

Preferably, the range of the first wavelength is 340nm-420nm, the range of the second wavelength is 520nm-590nm, and the range of the third wavelength is 660nm-800nm.
22. The method according to claim 22, wherein the obtaining the interference detection information of the sample to be tested comprises dispensing a part of the sample to be tested and the diluent into the second container.
The method of claim 25, further comprising dispensing another part of the sample to be tested and the detection reagent into the first container to prepare the test sample; and transporting the first container To the sample measurement position.
22. The method according to claim 22, wherein said obtaining the interference detection information of the sample to be tested comprises performing interference detection on the sample to be tested before preparing the measurement sample.
22. The method according to claim 22, wherein said obtaining the interference detection information of the sample to be tested comprises using the first intensity of light to detect the interference in the measurement sample at the measurement position of the sample.
28. The method according to any one of claims 22-28, further comprising outputting the sample detection result and interference detection information after analyzing the sample detection result.
The method according to any one of claims 22-29, wherein the interference detection information includes at least one of the absorbance of the sample to be tested to light of a preset wavelength or the luminous flux of the sample to be tested .
The sample analysis device of claim 22, wherein the sample to be tested is blood, and the interfering substance includes at least one of hemoglobin, bilirubin, and chyle.
A computer-readable storage medium, characterized by comprising a program, which can be executed by a processor to implement the method according to any one of claims 22-31