CN219891083U

CN219891083U - Biochip detecting device

Info

Publication number: CN219891083U
Application number: CN202222550800.3U
Authority: CN
Inventors: 楚婧娣; 苗鑫; 张鸿
Original assignee: Beijing Heguang Technology Co ltd
Current assignee: Beijing Heguang Technology Co ltd
Priority date: 2022-09-26
Filing date: 2022-09-26
Publication date: 2023-10-24
Anticipated expiration: 2032-09-26

Abstract

The utility model provides a biochip detection device, wherein the biochip detection device comprises a chip accommodating seat, a light source and a detection unit, a biochip to be detected can be arranged on the chip accommodating seat, wherein the light source is used for emitting detection light to the biochip to be detected, the detection unit is arranged adjacent to the light source so as to receive reflected light of the biochip to be detected, and the detection unit is used for acquiring spectrum information of the biochip to be detected and detecting the biochip to be detected according to the spectrum information.

Description

Biochip detecting device

Technical Field

The utility model relates to the technical field of biological detection, in particular to a biochip detection device.

Background

The biochip technology is a micro biochemical analysis system which integrates discontinuous analysis processes in the life science field on the surface of a silicon chip or a glass chip according to the principle of specific interaction among molecules by a micro technology so as to realize accurate, rapid and large-information detection of cells, proteins, genes and other biological components (biotic components). Depending on the biological material solidified on the chip, the biochip can be classified into a gene chip, a protein chip, a polysaccharide chip, and a neuron chip.

In order to accelerate the construction and perfection of the grading diagnosis and treatment system, the grading diagnosis and treatment of common diseases, frequently-occurring diseases and chronic diseases is taken as a break; the virus coated on the biochip is used for detecting the change of pathogens, assisting in clinical accurate treatment of infection challenges, rapidly diagnosing infection strains of patients, rapidly identifying pathogens, reducing clinical requirements of unnecessary antibiotics, and promoting rapid development of POCT industry and subdivision fields thereof.

Chemiluminescence, sequencing, digital PCR and the like in the field of in-vitro diagnosis are developed very mature, and the accuracy and the sensitivity reach high levels. However, these techniques have relatively high demands on personnel operations, sites and equipment, so they are used in relatively many centralized laboratory scenarios. There are many demands for decentralization in vitro diagnostics, such as the fast check market and the home market. To meet the needs of these two markets, some techniques are needed that are simple to operate, fast and efficient, and that are highly sensitive.

Disclosure of Invention

A main advantage of the present utility model is to provide a biochip detecting apparatus, wherein the biochip detecting apparatus can accurately, instantly, and rapidly detect a biochip through spectroscopic detection.

Another advantage of the present utility model is to provide a biochip detecting apparatus, wherein the biochip detecting apparatus can detect a biochip through a spectrum sensor, and a result of detecting the biochip includes whether a virus to be detected or how much virus is contained.

Another advantage of the present utility model is to provide a biochip detection apparatus that does not require expensive mechanical equipment and complex structures. The present utility model thus successfully provides a cost effective solution.

According to one aspect of the present utility model, a biochip detecting apparatus of the present utility model capable of achieving the foregoing objects and other objects and advantages includes:

a chip receiving seat in which a biochip to be detected can be placed;

a light source for emitting probe light to the biochip to be tested; and

the detection unit is arranged adjacent to the light source so as to receive the reflected light of the biochip to be detected, and is used for acquiring the spectrum information of the biochip to be detected and detecting the biochip to be detected according to the spectrum information.

According to one embodiment of the utility model, the light source is located above the chip accommodating seat, and the distance between the light source and the chip accommodating seat is within a set distance range.

According to one embodiment of the present utility model, the device further comprises a device host, the light source, the detection unit and the chip receiving seat are disposed on the device host, and the light source and the detection unit may be connected to the device host through wires, and the device host provides the light source and the detection unit with electric energy required for operation.

According to one embodiment of the utility model, the chip receiving seat is slidably disposed on the device host.

According to one embodiment of the utility model, the bottom of the chip accommodating seat is provided with a water outlet groove.

According to one embodiment of the utility model, the device host comprises a host bracket and a lampshade arranged above the host bracket, wherein the chip accommodating seat is arranged on the host bracket of the device host in a drawable manner, and the light source and the detection unit are arranged on the inner side of the lampshade.

According to one embodiment of the present utility model, the device host further includes a case and a card slot for accommodating the case, the case is located above the lamp cover, and the case may be inserted into the card slot.

According to one embodiment of the utility model, the light source is a broad spectrum light source.

According to one embodiment of the utility model, the distance between the detection unit and the biochip to be tested is within 5 mm.

According to one embodiment of the present utility model, the device further comprises a lens assembly, wherein the lens assembly is communicated with the light source, and the detection light emitted by the light source is projected to the biochip to be tested through the lens assembly; the lens component is communicated with the detection unit, and light reflected by the biochip to be detected is transmitted to the detection unit through the lens component so that the detection unit can acquire spectrum information of the biochip to be detected.

According to one embodiment of the present utility model, the lens assembly includes a transmitting lens, a receiving lens, and two optical fiber units connecting the transmitting lens and the receiving lens, wherein the optical fiber units conductively connect the transmitting lens and the light source.

According to one embodiment of the utility model, the device host further comprises a spacer, wherein the spacer is arranged between the light source and the detection unit.

Further objects and advantages of the present utility model will become fully apparent from the following description and the accompanying drawings.

These and other objects, features and advantages of the present utility model will become more fully apparent from the following detailed description and accompanying drawings.

Drawings

FIG. 1 is a cross-sectional view of a biochip detecting apparatus according to a first preferred embodiment of the utility model.

FIG. 2 is a side view of the biochip detecting apparatus according to the first preferred embodiment of the utility model.

FIG. 3 is a front view of the biochip detecting apparatus according to the first preferred embodiment of the utility model.

FIG. 4 is a schematic diagram of the detection principle of the biochip detection apparatus according to the first preferred embodiment of the utility model.

FIG. 5 is a schematic diagram showing a detection curve of the biochip detecting apparatus according to the first preferred embodiment of the utility model.

Fig. 6A and 6B are operation diagrams of the biochip detecting apparatus according to the first preferred embodiment of the utility model.

Fig. 7A to 7B are schematic diagrams of detection spectra of the biochip detection apparatus according to the first preferred embodiment of the present utility model.

Detailed Description

The following description is presented to enable one of ordinary skill in the art to make and use the utility model. The preferred embodiments in the following description are by way of example only and other obvious variations will occur to those skilled in the art. The basic principles of the utility model defined in the following description may be applied to other embodiments, variations, modifications, equivalents, and other technical solutions without departing from the spirit and scope of the utility model.

It will be appreciated by those skilled in the art that in the present disclosure, the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," etc. refer to an orientation or positional relationship based on that shown in the drawings, which is merely for convenience of description and to simplify the description, and do not indicate or imply that the apparatus or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore the above terms should not be construed as limiting the present utility model.

It will be understood that the terms "a" and "an" should be interpreted as referring to "at least one" or "one or more," i.e., in one embodiment, the number of elements may be one, while in another embodiment, the number of elements may be plural, and the term "a" should not be interpreted as limiting the number.

A biochip detecting apparatus according to a first preferred embodiment of the present utility model is illustrated in the following description with reference to fig. 1 to 7B of the drawings of the present specification. The biochip detecting apparatus includes a light source 10, a detecting unit 20, and a chip receiving seat 30, wherein a biochip to be detected can be received in the chip receiving seat 30, the light source 10 can emit detection light toward the biochip to be detected placed in the chip receiving seat 30, the detecting unit 20 receives the reflected light reflected by the biochip to be detected, and the detecting unit 20 detects according to the reflected light of the biochip to be detected.

The light source 10 is disposed adjacent to the detecting unit 20, for example, the light source 10 is disposed around the detecting unit 20, the light source 10 is located above the chip accommodating seat 30, and the distance between the light source 10 and the chip accommodating seat 30 is within a set distance range. The chip accommodating seat 30 has at least one clamping position 301, wherein the chip to be tested is fixed in the clamping position 301 of the chip accommodating seat 30 by the chip accommodating seat 30. It should be noted that the size of the clamping position 301 of the chip accommodating seat 30 is matched with the size of the chip to be tested.

It should be noted that, in the preferred embodiment of the present utility model, the light source 10 and the detection unit 20 are located above the chip receiving seat 30, and the light source 10 is disposed adjacent to the detection unit 20.

The biochip detecting apparatus further comprises a device host 40, the light source 10, the detecting unit 20 and the chip receiving seat 30 are disposed on the device host 40, and the light source 10 and the detecting unit 20 may be connected to the device host 40 through wires, and the device host 10 provides the light source 10 and the detecting unit 20 with electric power required for operation.

The chip accommodating seat 30 is a pull-out type base structure, the chip accommodating seat 30 is slidably disposed on the device host 40, the biochip to be tested is placed at the clamping position 301 of the chip accommodating seat 30, the chip accommodating seat 30 can be pushed to the device host 40, and the chip accommodating seat 30 is located at the corresponding positions of the light source 10 and the detecting unit 20. Preferably, in the preferred embodiment of the present utility model, the chip receiving seat 30 is a drawing type base for drawing out the biochip to be tested and pushing the biochip to a set position for detection.

The chip accommodating seat 30 may be pulled out from the device host 40, a slide rail or a pulley is disposed under the chip accommodating seat 30, the chip accommodating seat 30 is pulled out by the pulley or the slide rail, the biochip to be tested is placed in the clamping position 301 of the chip accommodating seat 30, the chip accommodating seat 30 with the biochip to be tested assembled is pushed to a set position after the biochip to be tested is placed, and the set position is located under the light source 10, so that the light emitted by the light source 10 just passes through the biochip to be tested and then reaches the detection unit 20. Further, a water outlet groove may be provided at the bottom of the chip receiving seat 30.

It is worth mentioning that since the spectrum is "DNA" for each substance, each substance corresponds to its own spectrum. In this preferred embodiment of the utility model, the detection unit 20 is implemented as a spectrum sensor, i.e. by means of which the biochip is detected, the result of which comprises whether or not the virus to be detected is contained or how much. It can be understood that the size of the chip accommodating seat 30 is adjustable, different clamping positions are provided, and different biochips can be correspondingly placed.

The device host 40 includes a host bracket 41 and a lamp cover 42 disposed above the host bracket 41, wherein the chip receiving seat 30 is drawably disposed at the host bracket 41 of the device host 40, the light source 10 and the detection unit 20 are disposed inside the lamp cover 42, the light source 10 and the detection unit 20 are protected by the lamp cover 42, and the influence of external stray light on the detection result is blocked by the lamp cover 42. The host bracket 41 of the device host 40 is used for supporting and fixing the chip accommodating seat 30, and the lampshade 42 is located above the host bracket 41.

As shown in fig. 1 to 3, the device host 40 further includes a case 44 and a card slot 45 for accommodating the case 44. The housing 44 is located above the lamp housing 42, and the housing 44 is inserted into the locking groove 45 and can be pulled out of the locking groove 45, i.e., the housing 44 is disposed in the locking groove 45 so as to be able to be pulled out. During the inspection, the case 44 needs to be taken out from the clamping groove 45, and after the inspection is finished, the case 44 can be inserted into the clamping groove 45, so that the size is reduced, and the case 44 is supported and protected by the clamping groove 45.

It should be noted that, in one embodiment of the present utility model, the light source 10 and the detection unit 20 are disposed in the case 44, when the case 44 is taken out of the card slot 45 during use, the case 44 is located directly above the lamp cover 42, and the light source 10 located in the case 44 can emit probe light through the lamp cover 42 toward the chip receiving seat 30.

The chip receiving seat 30 further includes a receiving tray 31 and a drain groove 32 formed under the receiving tray 31, wherein the catching position 301 is formed at one side of the receiving tray 31, and the catching position 301 faces the direction of the light source 10 and the detecting unit 20. The water leakage groove 32 is formed under the receiving tray 31 to collect and guide the liquid flowing down the receiving tray 31, such as water generated during the cleaning of the chip receiving socket 30, to the outside through the water leakage groove 32 under the receiving tray 31.

As shown in fig. 4, the biochip to be detected is placed on the accommodating tray 31 of the biochip detecting apparatus, and the biochip to be detected receives the set incident light emitted from the light source 10, and the wavelength range of the incident light is related to the viruses of the biochip to be detected. Preferably, in this preferred embodiment of the present utility model, the light source 10 is a broad spectrum light source, and further preferably may be provided as a halogen tungsten lamp.

The incident light is incident to the upper surface of the biochip to be detected, and the surface of the biochip is provided with corresponding viruses to be detected. When the incident light irradiates the biochip to be detected with virus, the incident light is absorbed and reflected by the surface, and the reflected light is received by the detection unit and generates spectral lines. And the spectrum line of the biochip with the virus is different from the spectrum of the detected virus, and the spectrum curves before and after the virus is detected on the biochip are compared to determine whether the biochip contains the virus. And each virus corresponds to different spectral lines, the corresponding absorption peaks are different, and the offset of the absorption peaks is different due to different contents of the viruses, so that the type and the quantity of the viruses can be confirmed, and the low-load viruses can be detected, as shown in fig. 5.

Preferably, in the preferred embodiment of the present utility model, the detecting unit 20 is fixed above the chip housing seat 30 by the device host 40, and the distance between the detecting unit 20 and the biochip to be detected is within 5mm (including 5 mm) during detection.

As shown in fig. 4, the biochip detecting apparatus further comprises a lens assembly 50, wherein the lens assembly 50 is in communication with the light source 10, and the probe light emitted from the light source 10 is projected to the biochip to be detected through the lens assembly 50; the lens assembly 50 is communicated with the detection unit 20, and the light reflected by the biochip to be detected is transmitted to the detection unit 20 through the lens assembly 50 so that the detection unit 20 can obtain the spectrum information of the biochip to be detected.

The lens assembly 50 comprises a transmitting lens 51, a receiving lens 52 and two optical fiber units 53 connecting the transmitting lens 51 and the receiving lens 52, wherein the optical fiber units 53 can be connected with the transmitting lens 51 and the light source 10 in a conductive way, and the light emitted by the light source 10 is conducted to the transmitting lens 51 through the optical fiber units 53 and then projected to the biochip to be tested through the transmitting lens 51; the reflected light reflected by the biochip to be tested is transmitted by the receiving lens 52 through the optical fiber unit 53 to the detecting unit 20, and then the spectrum detection is performed by the detecting unit 20 according to the detected reflected light of the biochip to be tested.

As a preference, in this preferred embodiment of the present utility model, the transmitting lens 51 and the receiving lens 52 of the lens assembly 50 are disposed directly above the receiving tray 31, and the distance between the lens assembly 50 and the biochip to be tested is not more than 0.5cm at the time of detection.

As shown in fig. 6, the biochip detecting apparatus further comprises a circuit board 60, wherein the circuit board 60 is disposed on the device host 40, and the circuit board 60 is electrically connected to the light source 10 and the detecting unit 20, and the circuit board 60 controls the operating states of the light source 10 and the detecting unit 20.

Preferably, in this preferred embodiment of the utility model, the light source 10 may be a broad spectrum LED, or other broad spectrum light source. The distance between the light source 10 and the detection unit 20 is related to the size and distance of the biochip to be measured. When the biochip to be detected is very small, typically a few millimeters in size, the light source is very close to the spectral detection unit. In this preferred embodiment of the utility model, the distance of the light source 10 from the detection unit 20 is small, i.e. the light source 10 is arranged adjacent to the detection unit 20, the light source 10 and the detection unit 20 being arranged on top of the device holder 41. The device host 40 further includes a spacer 43, wherein the spacer 43 is disposed between the light source 10 and the detecting unit 20, and the light source 10 and the detecting unit 20 are spaced apart by the spacer 43, so as to prevent the light emitted from the light source 10 from directly entering the detecting unit 20, thereby affecting the detection result. The partition 43 is provided on top of the apparatus holder 41, with the partition 43 being spaced between the shuttle Hu Guangyuan 10 and the detecting unit 20.

The detection spectra shown in fig. 7A to 7B are schematic diagrams of the fluctuation of the virus front-back measurement curve in the preferred embodiment of the present utility model: curve L1 is the pre-viral spectral curve and curve L2 is the post-viral spectral curve; if the right shift amplitude of the curve L2 is not large, the curve is identified as negative; if the right shift of the curve L2 is large, the right shift is correctly recognized as positive.

As shown in fig. 7B, the trough left shift is positive with a large magnitude, and negative if the left or right shift is very small or substantially no shift.

It will be appreciated by persons skilled in the art that the embodiments of the utility model described above and shown in the drawings are by way of example only and are not limiting. The objects of the present utility model have been fully and effectively achieved. The functional and structural principles of the present utility model have been shown and described in the examples and embodiments of the utility model may be modified or practiced without departing from the principles described.

Claims

1. A biochip testing apparatus comprising:

a chip receiving seat in which a biochip to be tested can be placed;

a light source for emitting probe light to the biochip to be tested; and

2. The biochip detection apparatus according to claim 1, wherein the light source is located above the chip housing seat and the light source is located within a set distance range from the chip housing seat.

3. The biochip detection apparatus according to claim 1, further comprising a device host, the light source, the chip housing seat being provided to the device host, the detection unit being placed above the chip housing seat, and the light source and the detection unit being connected to the device host.

4. The biochip detection apparatus according to claim 3, wherein the chip housing is slidably provided to the device host, the device host being provided with a slide rail, the chip housing moving along the slide rail.

5. The biochip detection apparatus according to claim 1, wherein a water outlet groove is provided at the bottom of the chip housing seat.

6. The biochip detection apparatus according to claim 3, wherein the device host comprises a host bracket and a lamp housing provided above the host bracket, wherein the chip receiving seat is drawably provided at the host bracket of the device host, and the light source and the detection unit are provided inside the lamp housing.

7. The biochip detecting apparatus according to claim 6, wherein the device host further comprises a housing and a card slot for accommodating the housing, the housing being located above the lamp housing, the housing being insertable into the card slot.

8. The biochip detection apparatus according to claim 6, wherein the light source is a broad spectrum light source.

9. The biochip detection apparatus according to claim 6, wherein the detection unit is within 5mm from the biochip to be detected.

10. The biochip detection apparatus according to any one of claims 6 to 9, further comprising a lens assembly, wherein the lens assembly is in communication with the light source, and probe light emitted from the light source is projected to the biochip to be detected via the lens assembly; the lens component is communicated with the detection unit, and light reflected by the biochip to be detected is transmitted to the detection unit through the lens component so that the detection unit can acquire spectrum information of the biochip to be detected.

11. The biochip detection apparatus according to claim 10, wherein the lens assembly comprises a transmitting lens, a receiving lens, and two optical fiber units connecting the transmitting lens and the receiving lens, wherein the optical fiber units conductively connect the transmitting lens and the light source.

12. The biochip detection apparatus according to claim 10, wherein the device host further comprises a spacer, wherein the spacer is disposed between the light source and the detection unit.