CN115244403A

CN115244403A - Sample analysis device and method

Info

Publication number: CN115244403A
Application number: CN202080098148.7A
Authority: CN
Inventors: 孙骁; 郭文恒; 司新春; 武振兴
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd; Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd; Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Priority date: 2020-04-13
Filing date: 2020-04-13
Publication date: 2022-10-25
Also published as: WO2021207896A1

Abstract

A sample analysis device and method, provide the first type and measure and position the second type; transporting the first container to a first type of test location and/or a second type of test location; illuminating a first intensity of light to a first type of measurement location and/or illuminating a second intensity of light to a second type of measurement location; the first intensity is less than the second intensity; acquiring optical detection information corresponding to the light with the first intensity and/or the light with the second intensity; and analyzing the detection result of the sample according to the optical detection information corresponding to the light with the first intensity and/or the light with the second intensity.

Description

Sample analysis device and method

Technical Field

The present invention relates to a sample analysis device and method.

Background

A sample analysis apparatus is a type of apparatus for analyzing biochemical characteristics of a sample, which is widely used in the field of clinical medicine to help medical staff diagnose a patient's condition. Taking a blood coagulation instrument as an example, the blood coagulation instrument can measure the blood coagulation time and the concentration or activity of related substances contained in the blood coagulation instrument; the coagulometer may detect coagulation items by an optical method, and specifically, the coagulometer irradiates light to a reaction cup solution during a reaction process and analyzes scattered or transmitted light to obtain optical information such as absorbance of the solution, so as to obtain coagulation time or concentration of a substance to be detected.

The optical method detects the coagulation item through optical information of scattering, reflection or transmission of light by the reaction solution, so that when a sample has an interfering substance, the scattering, reflection or transmission property of the reaction solution to the light changes, which affects the measurement, and the detection result is inaccurate, and even the detection result cannot be obtained under severe conditions.

Summary of The Invention

Technical problem

The invention mainly provides a sample analysis device and a sample analysis method.

Solution to the problem

Technical solution

According to a first aspect, there is provided in an embodiment a sample analysis device comprising:

the optical detection component comprises a first type of measurement and positioning and a first detector corresponding to the first type of measurement and positioning, a second type of measurement and positioning and a second detector corresponding to the second type of measurement and positioning;

the illumination component is used for providing light with first intensity to a first container which is positioned at the first type measuring position and is filled with a measuring sample, and providing light with second intensity to a first container which is positioned at the second type measuring position and is filled with the measuring sample; the determination sample is prepared from a sample and a reagent; the first intensity is less than the second intensity;

a transport component for transporting the first container to the first or second type of test location;

and the control component is used for controlling the conveying component to convey the first container filled with the determination sample to the first type determination position or the second type determination position according to the interferent detection information of the sample for optical detection.

In one embodiment, the optical detection component is configured to receive an output optical signal of the first container in the first type of measurement location or the second type of measurement location after being irradiated by the illumination component, and convert the output optical signal into a corresponding electrical signal; the output optical signal comprises at least one of transmitted light, reflected light, or scattered light;

the sample analyzing apparatus further includes an analyzing part for analyzing the electric signal to perform analysis of the sample test item.

In one embodiment, the sample analyzer further comprises an interferent detection means for interferent detection of the sample to obtain the interferent detection information, the interferent detection information being indicative of whether an interferent content of the sample exceeds a threshold, the control means being adapted to control the transport means to transport the first container to the first type of detection position if the interferent content of the sample does not exceed the threshold; or when the interferent content of the sample exceeds a threshold, controlling the transport component to transport the first container to the second type of measurement location.

In one embodiment, the interferer detection component comprises an interferer detection bit and a third detector located to one side of the interferer detection bit; the illumination component is used for illuminating a second container which is positioned at an interfering object detection position and at least contains a sample, and the interfering object detection component is used for receiving an output optical signal of the second container after the second container is illuminated by the illumination component so as to obtain interfering object detection information of the sample.

In one embodiment, the sample analyzer further includes a dispensing unit, and the control unit controls the dispensing unit to dispense a part of the sample and a diluent into the second container, and controls the dispensing unit to dispense another part of the sample and a detection reagent into the first container, thereby preparing the measurement sample.

In one embodiment, the first container is a reaction cup and the second container is a cuvette.

In one embodiment, the illumination component includes a first light source and a one-to-many fiber optic beam providing the first intensity of light to the first type of detection location and the interferer detection location, respectively.

In one embodiment, the illumination means further comprises a second light source for providing light of the second intensity to illuminate the first container containing the assay sample at the second type of assay site.

In one embodiment, the first light source is a multi-wavelength light source for providing light of at least a first wavelength, a second wavelength, and a third wavelength; preferably, the first wavelength is in the range of 340nm-420nm, the second wavelength is in the range of 520nm-590nm, and the third wavelength is in the range of 660nm-800nm.

In an embodiment, the second light source is a single-wavelength light source, and is configured to provide light with a fourth wavelength, where the fourth wavelength is not smaller than any one of the first wavelength, the second wavelength, or the third wavelength.

In one embodiment, the illumination component irradiates a first container located in the first type measurement location and containing a measurement sample through light with a first intensity, and the first detector is configured to receive an output optical signal of the first container irradiated by the illumination component, so as to obtain detection information of an interfering object of the sample.

In one embodiment, the sample is a blood sample and the interferent comprises at least one of hemoglobin, bilirubin, and chyle.

In one embodiment, the interferent detection information includes at least one of absorbance of the sample to light of different wavelengths or transmittance of the sample.

According to a second aspect, there is provided in an embodiment a sample analysis device comprising:

the optical detection component comprises a first type detection position and a first detector corresponding to the first type detection position, a second type detection position and a second detector corresponding to the second type detection position;

a transport member for transporting a container containing an assay sample to at least one of the first type of assay site or the second type of assay site for optical detection; the determination sample is prepared from a sample and a reagent;

the illumination component is used for providing light with first intensity to illuminate the first container which is positioned at the first type measuring position and is filled with the measuring sample, and providing light with second intensity to illuminate the first container which is positioned at the second type measuring position and is filled with the measuring sample; the first intensity is less than the second intensity; the first detector is used for acquiring first optical detection information corresponding to the light with the first intensity, and the second detector is used for acquiring second optical detection information corresponding to the light with the second intensity;

and the analysis component is used for analyzing the detection result of the sample according to at least one of the first optical detection information or the second optical detection information.

In one embodiment, the sample analysis apparatus further comprises a sample interference detection component for performing interference detection on the sample to obtain interference detection information of the sample, the interference detection information indicating whether the interference content of the sample exceeds a threshold, and a control component for controlling the transport component to transport the first container to the first type of detection location when the interference content of the sample does not exceed the threshold; or controlling the transport component to transport the first container to the second type of measurement location when the interferent content of the sample exceeds a threshold.

In one embodiment, the illumination component irradiates a first container which is positioned at the first type measurement position and contains the measurement sample through light with a first intensity, and the first detector is further used for receiving an output optical signal of the first container irradiated by the illumination component so as to obtain the luminous flux of the measurement sample; the transport means is for transporting the first container to the second type of assay location when the luminous flux of the assay sample does not exceed a threshold value.

According to a third aspect, an embodiment provides a method of sample analysis, comprising:

providing a first type of measurement and positioning and a second type of measurement and positioning;

dispensing a sample and a reagent into a first container to prepare a measurement sample;

transporting the first container to a first type of test location and/or a second type of test location;

illuminating a first intensity of light to a first type of measurement location and/or illuminating a second intensity of light to a second type of measurement location; the first intensity is less than the second intensity;

acquiring optical detection information corresponding to the light with the first intensity and/or the light with the second intensity;

and analyzing the detection result of the sample according to optical detection information corresponding to the light with the first intensity and/or the light with the second intensity.

In one embodiment, the sample analysis method further comprises performing interferent detection on the sample to obtain interferent detection information for the sample prior to transporting the first container to the first type of assay location and/or the second type of assay location, the interferent detection information being indicative of whether an interferent content of the sample exceeds a threshold; said transporting the first container to the first type of measurement location and/or the second type of measurement location comprises:

transporting the first container to the first type of location if the interferent content of the sample does not exceed a threshold; or

Transporting the first container to the second type of measurement location if the interferent content of the sample exceeds a threshold.

In one embodiment, the performing the interferent detection on the sample to obtain the interferent detection information comprises:

dispensing a portion of the sample and a diluent into a second container;

illuminating the second container with light of a first intensity;

receiving an output optical signal after illuminating the second receptacle;

converting the optical signal into an electrical signal;

and analyzing the electric signal to obtain the detection information of the interferent of the sample.

In one embodiment, the sample analysis method further comprises obtaining interferent detection information of the assay sample at the first type of assay location after transporting the first container to the first type of assay location, the interferent detection information being indicative of whether an interferent content of the sample exceeds a threshold; said transporting the first container to the first type of test location and/or the second type of test location comprises:

transporting the first container from the first type of measurement location to the second type of measurement location if the interferent content of the sample exceeds a threshold.

In one embodiment, said transporting the first container to the first type of test location and/or the second type of test location comprises:

transporting the first container to the first type of assay location for optical assay; and

transporting the first container to the second type of assay location for optical assay.

In an embodiment, the analyzing the detection result of the sample according to the optical detection information measured at the first type of detection location and/or the second type of detection location includes: selecting optical detection information measured at the first type measurement position or the second type measurement position according to the interferent detection information of the sample; and analyzing the detection result of the sample according to the selected optical detection information.

In one embodiment, the sample analysis method further comprises obtaining interferent detection information for the sample, the interferent detection information being indicative of whether an interferent content of the sample exceeds a threshold; the selecting optical detection information measured at the first type of measurement location or the second type of measurement location based on the interferent detection information for the sample comprises:

selecting optical detection information measured by the first type of measurement location if the interferent content of the sample does not exceed a threshold;

selecting optical detection information measured by the second type of measurement location if the interferent content of the sample exceeds a threshold.

Advantageous effects of the invention

Brief description of the drawings

Drawings

FIG. 1 is a schematic diagram showing absorption spectra of three interferents including hemoglobin, bilirubin and chyle to light in each wavelength range;

FIG. 2 is a schematic representation of the transmission response optical curves for a normal sample and a severe chyle sample according to one embodiment;

FIG. 3 is a schematic diagram of the transmission response optical curve of a heavy chylomicron sample at the anti-tamper determination location and at the conventional determination location according to an embodiment;

FIG. 4 is a schematic structural diagram of a sample analyzer according to an embodiment;

FIG. 5 is a schematic view of a sample analyzer according to another embodiment;

FIG. 6 is a schematic view of a sample analyzer according to yet another embodiment;

FIG. 7 is a schematic structural diagram of an illumination component according to an embodiment;

FIG. 8 is a schematic view of a structure of a lighting unit according to another embodiment;

FIG. 9 is a schematic structural view of a sample analyzer according to still another embodiment;

FIG. 10 is a schematic view of a sample analyzer according to still another embodiment;

FIG. 11 is a schematic view of a sample analyzer according to yet another embodiment;

FIG. 12 is a schematic flow chart of a sample analysis method according to an embodiment;

FIG. 13 is a schematic flow chart of a sample analysis method according to another embodiment;

fig. 14 is a schematic flow chart illustrating the process of performing the interferent detection on the sample to obtain the interferent detection information of the sample according to an embodiment;

FIG. 15 is a schematic flow chart of a sample analysis method according to yet another embodiment;

fig. 16 is a schematic flow chart of a sample analysis method according to still another embodiment.

Examples of the invention

Modes for carrying out the invention

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the description of the methods may be transposed or transposed in order, as will be apparent to a person skilled in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

In the optical measurement of blood coagulation, there are generally three specific methods: coagulation, immunoturbidimetry and chromogenic substrate methods. The chromogenic substrate method generally employs violet light or ultraviolet light AT 340nm to 420nm, and is generally used for the measurement of detection items such as antithrombin-III (AT-III or AT 3). Immunoturbidimetry typically uses a 520nm-590nm yellow-green light, and immunoturbidimetry is typically used for assays such as D-dimer (DD), fibrin/Fibrinogen Degradation Products (FDP), and the like. The coagulation method generally uses red light or infrared light of 660nm to 800nm, and is generally used for measuring detection items such as thrombocythemin time (PT), activated Partial Thromboplastin Time (APTT), thrombin Time (TT), and Fibrinogen (FIB).

In the optical method for measuring blood coagulation, whether it is a coagulation method, an immunoturbidimetry method or a chromogenic substrate method, when an interfering substance is present in a sample, the detection is interfered. Normally, the plasma sample is yellowish and almost transparent, but some patients suffer from diseases and other causes, such as jaundice, hemolysis or lipemia, and the plasma is brownish yellow, reddish or milky. The symptom of jaundice indicates that bilirubin is an interferent in the sample, the symptom of hemolysis indicates that hemoglobin is an interferent in the sample, and the symptom of lipemia indicates that chyle is an interferent in the sample. The absorption spectra of the three interferents are different, and when interferents such as hemoglobin, bilirubin and chyle exist in sample plasma, the interferents can strongly absorb light, so that the detection of the sample is interfered, and the detection result is biased. Hemoglobin, bilirubin, and chyle can generally be collectively referred to as HIL interference, where H refers to hemoglobin, I refers to bilirubin, and L refers to chyle. Bilirubin, hemoglobin and chyle, three interference substances present different colors, and absorption spectra thereof are shown in figure 1: bilirubin and hemoglobin have distinct absorption peaks, bilirubin has a strong absorption peak at about 450nm, hemoglobin has a strong absorption peak at about 420nm, both of which hardly absorb at a wavelength of 660nm or more, chyle has absorbance in all visible spectra, and absorbance decreases as the wavelength increases, but even at 800nm, the absorbance still remains constant. Therefore, as can be seen from the figure, the three interferents have strong absorption for light in a small wavelength band, especially light below 600nm, which greatly reduces the light transmittance of the mixture of the sample and the detection reagent, and the actually received light is very small, thereby affecting the accuracy and reliability of the optical method determination; sometimes the light that can be received is even almost zero, resulting in an unrecognizable course of reaction of the sample with the detection reagent.

One solution to the interferents is to provide light in a wavelength band that is not absorbed by the interferents in the sample for detection, e.g., to provide a larger wavelength such as 800nm to illuminate the mixture of sample and detection reagent, as is evident from the figure, hemoglobin and bilirubin have little absorption of light at wavelengths greater than 800nm, while chyle has relatively little absorption of light at wavelengths greater than 800nm. This approach can effectively exclude the effect of bilirubin and hemoglobin on the sample measurement, but can also have an effect on chylomicron samples, particularly those that are retried. Because although chyle has a relatively small absorption of light with a wavelength of more than 800nm, these absorptions of light by chyle are still not negligible in relation to the case where the detection result requires a relatively precise one; in addition, when the concentration of chyle in a sample is relatively high, even in a situation where the accuracy of the detection result requires general (for example, a physical examination test) the absorption of light by chyle is still not negligible, and chyle still seriously affects the accuracy of the detection result; the optical transmission response curves for the normal and severe chylomicron samples are shown in fig. 2, where the abscissa in fig. 2 is time in seconds and the ordinate is the light flux received by the transmission photodetector. As can be seen from FIG. 2, for the sample with severe chyle, due to its too low transmittance, the luminous flux of the reaction is almost zero all the time, and there is substantially no light transmission, i.e. the absorbance of the analyte is too large, exceeding the maximum absorbance test range of the optical sensor.

In addition, the above-mentioned scheme of measuring by switching light of a large wavelength is not applicable to items such as detection by a chromogenic substrate method, because the chromogenic substrate method is, in view of the detection principle, because a substance in a sample is replaced by a detection reagent after the sample and the detection reagent react, and the replaced substance absorbs only in the ultraviolet and violet ranges, it is generally possible to use only the above-mentioned violet light or ultraviolet light of 340nm to 420nm, and it is not possible to use light of other wavelength bands, and the coagulation method and immunoturbidimetric method can theoretically use light of other wavelength band ranges for detection in addition to the above-mentioned light of the respective wavelength band ranges.

The applicant researches the above problems and proposes another solution to solve the problem of the influence of the interferent in the sample on the item detection by increasing the light intensity to compensate the influence of the low light flux caused by the interferent. Specifically, a sample having a high content of interfering substances is specifically measured by introducing a specific type of measuring site, which provides a light having a higher intensity than that used for ordinary measurement. By improving the light intensity of irradiation, the sample with severe interference still has transmitted light and the like which meet the requirements to a certain extent, and the maximum absorbance test range of the sample can be improved so as to enhance the color anti-interference capability of the photoelectric detector.

In some embodiments, a second type of position measurement (or tamper resistant) may be introduced on the basis of a first type of position measurement (or regular position measurement) that is provided with light at a first intensity and a second type of position measurement that is provided with light at a second intensity greater than the first intensity. The transmitted light reaction curves of the severe chyle sample in the anti-interference measurement and positioning and the conventional measurement and positioning are shown in fig. 3, and it can be seen that the initial light intensity of the conventional measurement and positioning is weaker, the received luminous flux is almost zero, and the reaction can still be detected in the anti-interference measurement and positioning. The invention will be specifically explained below.

In some embodiments, a sample analysis device is disclosed. A sample analysis device is an instrument for analyzing and measuring a sample. The flow of the test of the sample analyzer is not exemplified by a blood coagulation analyzer (i.e., a blood coagulation analyzer referred to herein). The test procedure for coagulation analyzers is generally as follows: the blood coagulation analyzer can irradiate the measurement sample in the cuvette with, for example, light of multiple wavelengths, and analyze the measurement sample by a coagulation method, an immunoturbidimetry method, a chromogenic substrate method, or the like to obtain a coagulation reaction curve of the measurement sample with time, thereby further calculating the coagulation time or other coagulation-related performance parameters of the measurement sample.

Referring to fig. 4, 5 and 6, the sample analyzer in some embodiments may include an optical detection unit 10, a lighting unit 20 and a transportation unit 30, and in some embodiments may further include a control unit 40 and/or an analysis unit 50, which are described in detail below.

The optical detection component 10 includes a first type measurement location 01, a first detector 11 corresponding to the first type measurement location 01, a second type measurement location 02, and a second detector 12 corresponding to the second type measurement location 02.

In some embodiments, the first type of measurement location 01 is one or more. In some embodiments, the second type of measurement location 02 is one or more.

In some examples, the first detector 11 and the second detector 12 may be implemented by a component capable of converting an optical signal into an electrical signal, such as a photodetector. Specifically, the first detector 11 and the second detector 12 may be photodiodes PD, photomultiplier tubes PMT, avalanche photodiodes APD, charge coupled devices CCD, complementary metal oxide semiconductor CMOS, image enhancement type detectors ICCD, electron multiplication type EMCCD, or the like.

The optical detection unit 10 is adapted to cooperate with the illumination unit 20. In some embodiments, the optical detection component 10 is configured to receive an output optical signal of the first container illuminated by the illumination component 20 at the first type measurement location 01 or the second type measurement location 02, and convert the output optical signal into a corresponding electrical signal; the output optical signal includes at least one of transmitted light, reflected light, or scattered light. The electrical signal is used to analyze the sample. In some embodiments, the output optical signal is transmitted light.

The light irradiation unit 20 is used to supply light for measurement. In some embodiments, the illumination component 20 is capable of providing two intensities of light, for example, a first intensity of light and a second intensity of light, wherein the first intensity is less than the second intensity. The light of the first intensity may be the intensity of light normally used for measuring a sample, and the light of the second intensity may be light having a higher intensity than the light of the first intensity, and may be used for measuring a sample containing an interfering substance in the present invention. In some embodiments, the illumination assembly 20 is configured to provide a first intensity of light to a first container (e.g., cuvette) containing an assay sample at a first type of assay site 01, and a second intensity of light to a first container containing an assay sample at a second type of assay site 02; accordingly, the first detector 11 is configured to obtain first optical detection information corresponding to the first intensity of light, and the second detector 12 is configured to obtain second optical detection information corresponding to the second intensity of light; wherein an assay sample is prepared from a sample and a reagent (or detection reagent).

The illumination means 20 may comprise two light sources, one for providing a first intensity of light to the first type of measurement site 01 and another for providing a second intensity of light to the second type of measurement site 02, as explained in more detail below.

In some embodiments, referring to fig. 7, the illumination device 20 includes a first light source 21 and a light-splitting fiber bundle 22 for providing light of a first intensity to the first type measurement site 01. In some embodiments comprising the interferent detection bits 03, which interferent detection bits 03 are also referred to in detail below, the illumination means 20 comprises a first light source 21 and a split-beam optical fiber bundle 22 providing light of said first intensity to the first type of detection bit 01 and to said interferent detection bits 03. In some embodiments, the first light source 21 is a multi-wavelength light source for providing light of at least a first wavelength, a second wavelength, and a third wavelength. In some preferred embodiments, the first wavelength is in the range of 340nm to 420nm, the second wavelength is in the range of 520nm to 590nm, and the third wavelength is in the range of 660nm to 800nm. By providing light of multiple wavelengths to the first type of assay site 01, it is possible to allow the same first type of assay site 01 to carry out detection of a plurality of items, for example, detection items supporting assays such as by coagulation, immunoturbidimetry, chromogenic substrates, and the like. In some embodiments, the first light source 21 sequentially outputs the light of the first wavelength and the first intensity, the light of the second wavelength and the first intensity, and the light of the third wavelength and the first intensity according to a predetermined compliance in one illumination period. The above is an example of providing a plurality of first type measurement locations 01 with light of a first intensity by means of a first light source 21 and a one-to-many fiber optic bundle 22. Each optical fiber at the output end of the light splitting/multiplexing optical fiber bundle 22 corresponds to one first-type measuring position 01, so that a plurality of first-type measuring positions 01 can be formed, and because the same first light source 21 is adopted, basically, no difference exists between the first-type measuring positions 01. The first light source 21 may include a plurality of single-wavelength light sources to form a structure capable of emitting light of multiple wavelengths, or the first light source 21 may be implemented by a broad-spectrum white light source such as a halogen lamp or a white light lamp, for example, by matching with a rotatable optical filter, light of different wavelengths may be emitted in time division within one period. In some embodiments, each first type measurement location 01 may also be provided with a first light source 21, each first light source 21 being capable of providing light of a different wavelength to the corresponding first type measurement location 01.

In some embodiments, referring to fig. 8, the illumination assembly 20 further comprises a second light source 23 for providing a second intensity of light to the first container containing the assay sample at the second type of assay site 02. In some embodiments, the second light source 23 is a single wavelength light source for providing light at a fourth wavelength. In some embodiments the fourth wavelength may be equal to any of the first wavelength, the second wavelength, or the third wavelength. In some embodiments, the fourth wavelength is no less than any of the first, second or third wavelengths, for example the fourth wavelength may be 800nm. In other embodiments, the second light source 23 may also be a multi-wavelength light source for providing light of at least a first wavelength, a second wavelength, and a third wavelength; in some embodiments, the second light source 23 sequentially outputs the light of the first wavelength and the second intensity, the light of the second wavelength and the second intensity, and the light of the third wavelength and the third intensity according to a predetermined compliance in one illumination period. To improve the light performance, the second light source 23 may be optically shaped in relation to illuminate the second type of measurement location 02.

As can be seen from the above description, the first type measurement location 01 is provided with light of a first intensity, the second type measurement location 02 is provided with light of a second intensity, and generally, the first type measurement location 01 is a sample with a relatively small content of normal sample or interfering substance, so the first type measurement location 01 is generally provided in plurality. All the first-type measurement sites 01 in the sample analyzer can share one light source by one-to-many fiber bundles, and from another perspective, all the first-type measurement sites 01 in the sample analyzer are also limited by the shared light source, so that it is not easy to adjust the intensity of light emitted from the shared light source to be relatively high (for example, the second intensity), because this would affect the optical detection of normal measurement samples on all the first-type measurement sites 01, and therefore, it is a reasonable and excellent design to set the first-type measurement sites 01 to be provided with light of the first intensity, and then introduce the second-type measurement sites 02 to be provided with light of the second intensity.

The transport means 30 are used to transport the first container to the first type measuring station 01 or the second type measuring station 02. Specifically, the transport member 30 transports the first container containing the measurement sample to at least one of the first-type measurement site 01 or the second-type measurement site 02 for optical detection.

The control unit 40 is used to control the operation of the transport unit 30, and for example, the control unit may control the transport unit 30 to transport the first container containing the measurement sample to the first type measurement site 01 or the second type measurement site 02 for optical detection based on the interfering object detection information of the sample.

The analyzing part 50 is used to analyze the electrical signal output from the optical detecting part to perform analysis of the sample test item. In some embodiments, the analyzing component 50 is configured to analyze the detection result of the sample according to at least one of the first optical detection information or the second optical detection information.

In the present invention, a first container containing a measurement sample may be transported to the first type measurement site 01 and the second type measurement site 02 to be optically measured, and then at least one of the first optically measured information and the second optically measured information may be selected according to a predetermined condition, for example, a condition related to the sample interfering substance detection information, to analyze the detection result of the sample. Alternatively, the first container containing the assay sample may be selectively transported to one of the first type assay site 01 and the second type assay site 02 for optical detection based on a predetermined condition, for example, a condition related to the sample interferent detection information.

In some embodiments, the interferent detection information comprises at least one of absorbance of light by the sample at different wavelengths, or an amount of transmission. Taking the light absorbance as an example, the light absorbance of the sample to different wavelengths represents the light absorption degree of the sample to be detected when the sample to be detected is irradiated by light; if the absorbance of the sample to the preset wavelength exceeds a preset absorbance threshold value, for example, the absorbance of at least one of 405nm, 575nm, 660nm and 800nm exceeds a corresponding absorbance threshold value, it indicates that the interferent of the sample to be detected exceeds the threshold value of the interferent. Taking the light transmittance of the sample as an example, the light transmittance of the sample represents the degree of light passing through the sample to be detected when the sample to be detected is irradiated with light, and the light transmittance of the sample to be detected can be the initial light flux detected before the sample to be detected is subjected to formal coagulation item detection; and if the initial luminous flux of the sample to be detected is lower than the preset luminous flux threshold, indicating that the interferent of the sample to be detected exceeds the threshold of the interferent.

It can be seen that, in either mode, the detection of the interfering substance with respect to the sample is involved, and therefore, how to detect the interfering substance with respect to the sample will be described below.

Referring to fig. 9, in some embodiments, the sample analysis apparatus may further include an interferent detection unit 60, where the interferent detection unit 60 is configured to perform interferent detection on the sample to obtain interferent detection information, and the interferent detection information is configured to indicate whether the interferent content of the sample exceeds a threshold. The interfering object detecting means 60 can be implemented in various ways, for example, referring to fig. 10, the interfering object detecting means 60 can comprise an interfering object detecting bit 03 and a third detector 13 located at one side of said interfering object detecting bit. The third detector 13 may be implemented by a component capable of converting an optical signal into an electrical signal, such as a photodetector or the like. Specifically, the third detector 13 may be a photodiode PD, a photomultiplier tube PMT, an avalanche photodiode APD, a charge coupled device CCD, a complementary metal oxide semiconductor CMOS, an image enhancement type detector ICCD, an electron multiplication type EMCCD, or the like. The illumination means 20 is for illuminating a second container (e.g., a cuvette, or the like) containing at least the sample at the interfering substance detection site — for example, the illumination means 20 is illuminated by light of a first intensity; the third detector 13 is configured to receive an output optical signal of the second container irradiated by the illumination component 20, so as to obtain detection information of an interfering object of the sample to be detected.

The introduction of the interference detection component 60 can detect the interferent in the sample to be detected, and obtain the detection information of the sample interferent. In other embodiments, the interferent detection may be performed on the sample to be detected at the first type location 01 without providing any interferent detection bits, which is described in detail below.

In some embodiments, the illumination component 20 illuminates a first container containing a measurement sample and located at the first type measurement location 01 through light of a first intensity, and the first detector 11 is configured to receive an output optical signal of the first container illuminated by the illumination component 20, so as to obtain detection information of an interfering object of the sample to be measured; the interferent detection information is used for judging whether the interferent of the sample to be detected exceeds a threshold value. Specifically, the interferent detection information may be acquired using the average light flux of the first container containing the measurement sample, which is the time period after the first-type measurement site 01 is placed and before the start of the test. In one example, when the sample is added to the last step of the trigger reagent, the mixing and moving of the mixture to the first type measurement position 01 can be completed within generally 3s, and then the detection is started at the 10 th s, and in 7s between the 3 rd s and the 10 th s, the illumination component 20 irradiates a first container containing the measurement sample at the sample measurement position with light of a first intensity, and the first detector 11 is used for receiving an output light signal of the first container irradiated by the illumination component 20, for example, the minimum light transmittance of the average light flux in the period of time, so as to obtain the interferent detection information of the sample to be detected.

In the above embodiments of detecting a sample interfering substance, it can be understood that a person skilled in the art may also use other methods to detect a sample interfering substance, for example, by taking a picture of a sample to be detected, obtaining an image of the sample to be detected, and then analyzing the image by a method such as machine learning, so as to obtain the detecting information of the sample to be detected.

While some basic structures of the sample analyzer are described above, referring to fig. 11, some embodiments of the sample analyzer further include other components and structures, which are described in detail below.

The sample cell 80 is used to carry a sample. In some examples, the Sample unit 80 may include a Sample Delivery Module (SDM) and a front end track; in other examples, the sample cell 10 may be a sample tray, such as the example shown in fig. 11, which includes a plurality of sample sites for placing containers, and the sample tray can dispatch the sample to a corresponding position, such as a position for the pipetting device 70 to aspirate the sample, by rotating the tray structure.

The reagent unit 82 is used to carry reagents. In one embodiment, the reagent unit 82 is disposed in a disc-shaped structure, the reagent unit 82 has a plurality of positions for holding reagent containers, and the reagent unit 82 can rotate and drive the reagent containers held by the reagent unit 82 to rotate, so as to rotate the reagent containers to the reagent sucking position, so that the dispensing component 70 sucks reagent. The number of the reagent units 82 may be one or more.

The dispensing member 70 is used to aspirate and discharge a sample or a reagent. In some embodiments, the dispensing member 70 may include a sample dispensing member 71 and/or a reagent dispensing member 73. The sample dispensing member 71 is used to aspirate and discharge a sample into, for example, a first container to be loaded. In some embodiments, the sample dispensing member 71 may include a sample needle that performs a two-dimensional or three-dimensional motion in space by a two-dimensional or three-dimensional driving mechanism, so that the sample needle can move to suck the sample carried by the sample cell 80, and move to a position where the sample is to be loaded, for example, the first container, and discharge the sample into the first container. In some embodiments, the reagent dispensing unit 73 may include a reagent needle that performs a two-dimensional or three-dimensional motion in space by a two-dimensional or three-dimensional driving mechanism, so that the reagent needle can move to aspirate a reagent carried by the reagent unit 82 and to a position of a reagent to be added, for example, a first container, and discharge the reagent to the first container. In some embodiments, the sample dispensing component 71 and the reagent dispensing component 73 may also share a set of driving mechanism and a needle, and when the sample dispensing component 71 and the reagent dispensing component 73 share a set of driving mechanism and a needle, cleaning of the needle is required when a sample and a reagent are sucked, so as to avoid problems such as cross contamination.

In fig. 4, the reaction member 84 is used to carry an assay sample prepared from a sample and a detection reagent. In one example, the reaction part 84 is configured in a disc-shaped structure and has a plurality of placing positions for placing a first container, such as a reaction cup, and the reaction part 84 can rotate and drive the reaction cup in the placing position to rotate for scheduling the reaction cup in the reaction tray and incubating a mixed solution in the reaction cup. The first type measurement location 01 and the second type measurement location 02 may be on the reaction component 84, i.e., some placement locations on the reaction component 84 are the first type measurement location 01 and the second type measurement location 02; the first type measuring site 01 and the second type measuring site 02 may also be provided independently of the reaction component 84, i.e. at a certain position, for example close to the reaction component 84.

The following describes a test flow of the sample analyzer.

In some embodiments having the interference detection unit 60, the control unit 40 may control a dispensing unit 70 to dispense a portion of the sample or the diluent to a second container (e.g., cuvette). The illumination means 20 irradiates a second container containing at least a sample (a sample or a mixture of a sample and a diluent) and located at the interfering substance detection site 03, and the interfering substance detection means 60 receives an output optical signal of the second container irradiated by the illumination means 20 to acquire interfering substance detection information of the sample. Next, the following first or second mode may be performed.

In the first embodiment, the control unit 40 controls the dispensing unit 70 to dispense the other part of the sample and the detection reagent into the first container to prepare the measurement sample. When the content of the interferent in the sample does not exceed the threshold value, the control component 40 controls the conveying component 30 to convey the first container to the first type detection position 01 for optical detection; when the interferent content of the sample exceeds the threshold value, the control means 40 controls the transport means 30 to transport the first container to the second type of measuring position 02 for optical detection.

In the second embodiment, the control unit 40 controls the dispensing unit 70 to dispense the other part of the sample and the detection reagent into the first container to prepare the measurement sample. The transport member 30 transports the first container to the first type measuring station 01 for optical detection to obtain first optical detection information, and to the second type measuring station 02 for optical detection to obtain second optical detection information. The analysis component 50 selects the first optical detection information to analyze the detection result of the sample when the content of the interferent in the sample does not exceed the threshold; the analysis section 50 selects the second optical detection information to perform detection result analysis on the sample when the interferent content of the sample exceeds a threshold value.

In the embodiment of detecting the interferent in the sample at the first type measurement location 01, the illumination component 20 irradiates the first container containing the measurement sample at the first type measurement location 01 with light of the first intensity, and the first detector 11 receives the output optical signal of the first container irradiated by the illumination component 20 to obtain the interferent detection information of the sample. When the content of the interfering substance is judged not to exceed the threshold value according to the interfering substance detection information of the sample, the measurement sample is optically detected at the first type measurement location 01, and if the content of the interfering substance exceeds the threshold value, the first container is conveyed to the second type measurement location 02 by the conveying part 30 to be optically detected.

The sample herein may be a blood sample and the interferent comprises at least one of hemoglobin, bilirubin, and chyle. The interferent detection information herein may comprise at least one of absorbance of light, or transmission of light, by the sample at different wavelengths. For example, in the embodiment of detecting the interfering substance in the sample through the first type measuring position 01, the illumination unit 20 illuminates the first container containing the measuring sample and the first type measuring position 01 through the light with the first intensity, and the first detector 11 receives the output optical signal of the first container illuminated by the illumination unit 20 to obtain the luminous flux of the measuring sample; when the light flux of the measurement sample exceeds a threshold value, the measurement sample is optically detected at the first-type measurement site 01, whereas if the light flux of the measurement sample does not exceed the threshold value, the transport member 30 transports the first container to the second-type measurement site 02 for optical detection.

The above are some of the descriptions of the sample analyzing apparatus. Also disclosed in some embodiments of the present invention is a method of sample analysis that provides a first type of position measurement and a second type of position measurement. Referring to fig. 12, a method for sample analysis according to some embodiments includes the following steps:

step 110: the sample and the reagent are dispensed into the first container to prepare a measurement sample.

Step 120: the first container is transported to the first type of test location and/or the second type of test location.

Step 130: illuminating a first intensity of light to the first type of measurement location and/or illuminating a second intensity of light to the second type of measurement location; the first intensity is less than the second intensity.

Step 140: and acquiring optical detection information corresponding to the first intensity of light and/or the second intensity of light.

Step 150: and analyzing the detection result of the sample according to optical detection information corresponding to the light with the first intensity and/or the light with the second intensity.

Further flows and relationships between the steps are specifically described below.

Referring to fig. 13, a method for sample analysis according to some embodiments includes the following steps:

step 100: prior to transporting the first container to the first type of assay location and/or the second type of assay location, the method further includes performing interferent detection on the sample to obtain interferent detection information for the sample, the interferent detection information indicating whether an interferent content of the sample exceeds a threshold value, at step 120. Referring to fig. 14, the step 100 of performing the interferent detection on the sample to obtain the interferent detection information of the sample includes: step 10, dispensing a part of the sample and a diluent into a second container; 102, irradiating the second container with light with a first intensity; step 103, receiving an output optical signal after irradiating the second container; step 104, converting the optical signal into an electrical signal; and 105, analyzing the electric signal to obtain the detection information of the interferent of the sample.

Step 110: the sample and the reagent are dispensed into the first container to prepare a measurement sample. For example, step 110 dispenses the reagent and the sample remaining in step 101 into a first container to prepare an assay sample.

Step 120: the first container is transported to the first type of test location and/or the second type of test location. Specifically, if the interferent content of the sample does not exceed a threshold, step 121 transports the first container to the first type of assay location for optical detection; if the interferent content of the sample exceeds a threshold, step 122 transports the first container to the second type of testing location for optical detection.

If the first container is transported to the first type testing position for optical testing in step 120, step 130 irradiates a first intensity of light to the first type testing position, obtains optical testing information corresponding to the first intensity of light in step 140, and analyzes the testing result of the sample according to the optical testing information corresponding to the first intensity of light in step 150.

If the first container is transported to the second type measuring position for optical detection in step 120, step 130 irradiates a second intensity of light to the second type measuring position, obtains optical detection information corresponding to the second intensity of light in step 140, and analyzes the detection result of the sample according to the optical detection information corresponding to the second intensity of light in step 150.

Referring to fig. 15, a method for sample analysis according to some embodiments includes the following steps:

step 110: the sample and the reagent are dispensed into the first container to prepare an assay sample.

Step 121: the first container is transported to a first type of test location.

Step 122: and after the first container is conveyed to the first type detection location, obtaining the detection information of the interferent of the detection sample at the first type detection location, wherein the detection information of the interferent is used for indicating whether the content of the interferent in the sample exceeds a threshold value.

Step 123: optically detecting the assay sample in the first container at the first type of assay location if the interferent content of the sample does not exceed a threshold. Step 130 irradiates a first intensity of light to a first type of measurement location, and obtains optical detection information corresponding to the first intensity of light in step 140, and performs a detection result analysis on the sample according to the optical detection information corresponding to the first intensity of light in step 150.

Step 125: transporting the first container from the first type of assay location to the second type of assay location for optical detection if the interferent content of the sample exceeds a threshold. Step 130 irradiates a second intensity of light to the second type of measurement location, and obtains optical detection information corresponding to the second intensity of light in step 140, and performs a detection result analysis on the sample according to the optical detection information corresponding to the second intensity of light in step 150.

Referring to fig. 16, a method for sample analysis according to some embodiments includes the following steps:

Step 120: the first container is transported to the first type of test location and/or the second type of test location. Specifically, step 120 transports the first container to the first type of assay location for optical assay; and transporting the first container to the second type of measurement location for optical measurement.

Step 130: illuminating a first intensity of light to the first type of measurement location and a second intensity of light to the second type of measurement location; the first intensity is less than the second intensity.

Step 140: optical detection information corresponding to the first intensity of light and the second intensity of light is acquired.

Step 150: and analyzing the detection result of the sample according to the optical detection information corresponding to the light with the first intensity and the light with the second intensity. Specifically, step 150 selects the optical detection information measured at the first type measurement location or the second type measurement location according to the interferent detection information of the sample, for example, when the interferent content of the sample does not exceed a threshold, step 150 selects the optical detection information measured at the first type measurement location; when the interferent content of the sample exceeds a threshold, step 150 selects optical detection information measured by the second type of measurement location; step 150 then analyzes the test results of the sample based on the selected optical test information.

The above is a description of the method of sample analysis according to some embodiments of the present invention.

Reference is made herein to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications may be made to the exemplary embodiments without departing from the scope hereof. For example, the various operational steps, as well as the components used to perform the operational steps, may be implemented in differing ways depending upon the particular application or consideration of any number of cost functions associated with operation of the system (e.g., one or more steps may be deleted, modified or incorporated into other steps).

In the above embodiments, all or part of the implementation may be realized by software, hardware, firmware, or any combination thereof. Additionally, as will be appreciated by one skilled in the art, the principles herein may be reflected in a computer program product on a computer readable storage medium, which is pre-loaded with computer readable program code. Any tangible, non-transitory computer-readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-to-ROM, DVD, blu-Ray discs, etc.), flash memory, and/or the like. These computer program instructions may be loaded onto a general purpose computer, special purpose computer, or other programmable data processing apparatus to produce a machine, such that the instructions which execute on the computer or other programmable data processing apparatus create means for implementing the functions specified. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including means for implementing the function specified. The computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified.

While the principles herein have been illustrated in various embodiments, many modifications of structure, arrangement, proportions, elements, materials, and components particularly adapted to specific environments and operative requirements may be employed without departing from the principles and scope of the present disclosure. The above modifications and other changes or modifications are intended to be included within the scope of this document.

The foregoing detailed description has been described with reference to various embodiments. However, one skilled in the art will recognize that various modifications and changes may be made without departing from the scope of the present disclosure. Accordingly, the disclosure is to be considered in an illustrative and not a restrictive sense, and all such modifications are intended to be included within the scope thereof. Also, advantages, other advantages, and solutions to problems have been described above with regard to various embodiments. However, the benefits, advantages, solutions to problems, and any element(s) that may cause any element(s) to occur or become more pronounced are not to be construed as a critical, required, or essential feature or element of any or all the claims. As used herein, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, system, article, or apparatus. Furthermore, the term "coupled," and any other variation thereof, as used herein, refers to a physical connection, an electrical connection, a magnetic connection, an optical connection, a communicative connection, a functional connection, and/or any other connection.

Those having skill in the art will recognize that many changes may be made to the details of the above-described embodiments without departing from the underlying principles of the invention. Accordingly, the scope of the invention should be determined only by the claims.

Claims

A sample analysis apparatus, comprising:

the optical detection component comprises a first type detection position and a first detector corresponding to the first type detection position, a second type detection position and a second detector corresponding to the second type detection position;

the illumination component is used for providing light with first intensity to a first container which is positioned at the first type measuring position and is filled with a measuring sample, and providing light with second intensity to a first container which is positioned at the second type measuring position and is filled with the measuring sample; the measuring sample is prepared from a sample and a reagent; the first intensity is less than the second intensity;

a transport component for transporting the first container to the first or second type of test location;

and the control component is used for controlling the conveying component to convey the first container filled with the determination sample to the first type determination position or the second type determination position according to the interferent detection information of the sample for optical detection.
The sample analysis device of claim 1, wherein the optical detection component is configured to receive an output optical signal from the first container in the first type of assay site or the second type of assay site after illumination by the illumination component and convert the output optical signal to a corresponding electrical signal; the output optical signal comprises at least one of transmitted light, reflected light, or scattered light;

the sample analysis device further includes an analysis section for analyzing the electric signal to perform analysis of a sample test item.
The sample analyzing apparatus according to claim 1 or 2, further comprising an interferent detection means for performing interferent detection on the sample to obtain the interferent detection information, the interferent detection information being indicative of whether an interferent content of the sample exceeds a threshold, the control means being adapted to control the transport means to transport the first container to the first type of location when the interferent content of the sample does not exceed the threshold; or when the interferent content of the sample exceeds a threshold, controlling the transport component to transport the first container to the second type of measurement location.
The sample analysis device of claim 3, wherein the interferent detection means comprises an interferent detection bit and a third detector located to one side of the interferent detection bit; the illumination component is used for illuminating a second container which is positioned at an interfering object detection position and at least contains a sample, and the interfering object detection component is used for receiving an output optical signal of the second container after the second container is illuminated by the illumination component so as to obtain interfering object detection information of the sample.
The sample analyzer according to claim 4, further comprising a dispensing unit, wherein the control unit controls the dispensing unit to dispense a part of the sample and a diluent into the second container, and controls the dispensing unit to dispense another part of the sample and a detection reagent into the first container, thereby preparing the measurement sample.
The sample analysis device of claim 4 or 5, wherein the first container is a reaction cuvette and the second container is a cuvette.
The sample analysis device of any one of claims 4-6, wherein the illumination means comprises a first light source and a one-to-many fiber optic beam providing light of the first intensity to the first type of detection site and the interferent detection site, respectively.
The sample analyzing apparatus according to claim 1 or 7, wherein the illumination means further comprises a second light source for providing light of the second intensity to illuminate the first container containing the assay sample at the second type of assay site.
The sample analyzing apparatus of claim 8, wherein the first light source is a multi-wavelength light source for providing light of at least a first wavelength, a second wavelength, and a third wavelength; preferably, the first wavelength is in the range of 340nm to 420nm, the second wavelength is in the range of 520nm to 590nm, and the third wavelength is in the range of 660nm to 800nm.
The sample analysis device of claim 9, wherein the second light source is a single wavelength light source for providing light at a fourth wavelength that is not less than any of the first, second, or third wavelengths.
The sample analyzer of claim 1 wherein the illumination assembly illuminates a first container containing an assay sample at the first type of assay location with light of a first intensity, and the first detector is configured to receive an output optical signal of the first container illuminated by the illumination assembly to obtain the analyte detection information of the sample.
The sample analysis device of any of claims 1-11, wherein the sample is a blood sample and the interferent comprises at least one of hemoglobin, bilirubin, and chyle.
The sample analysis device of any of claims 1-12, wherein the interferent detection information comprises at least one of absorbance of light, or transmission of light, by the sample at different wavelengths.
A sample analysis apparatus, comprising:

the optical detection component comprises a first type of measurement and positioning and a first detector corresponding to the first type of measurement and positioning, a second type of measurement and positioning and a second detector corresponding to the second type of measurement and positioning;

a transport member for transporting a container containing an assay sample to at least one of the first type of assay site or the second type of assay site for optical detection; the determination sample is prepared from a sample and a reagent;

the illumination component is used for providing light with first intensity to a first container which is positioned at the first type measuring position and is filled with a measuring sample, and providing light with second intensity to a first container which is positioned at the second type measuring position and is filled with the measuring sample; the first intensity is less than the second intensity; the first detector is used for acquiring first optical detection information corresponding to the light with the first intensity, and the second detector is used for acquiring second optical detection information corresponding to the light with the second intensity;

and the analysis component is used for analyzing the detection result of the sample according to at least one of the first optical detection information or the second optical detection information.
The sample analyzing apparatus according to claim 14, further comprising a sample interference detecting means for performing interference detection on the sample to obtain interference detection information of the sample, the interference detection information indicating whether an interference content of the sample exceeds a threshold value, and a control means for controlling the transport means to transport the first container to the first type of measurement location when the interference content of the sample does not exceed the threshold value; or controlling the transport component to transport the first container to the second type of measurement location when the interferent content of the sample exceeds a threshold.
The sample analyzer of claim 14 wherein the illumination assembly illuminates a first container containing the assay sample at the first type of assay site with light of a first intensity, the first detector further receiving an output optical signal from the first container illuminated by the illumination assembly to obtain a luminous flux of the assay sample; the transport means is for transporting the first container to the second type of assay location when the luminous flux of the assay sample does not exceed a threshold value.
A method of sample analysis, comprising:

providing a first type of measurement and positioning and a second type of measurement and positioning;

dispensing a sample and a reagent into a first container to prepare an assay sample;

transporting the first container to a first type of test location and/or a second type of test location;

illuminating a first intensity of light to the first type of measurement location and/or illuminating a second intensity of light to the second type of measurement location; the first intensity is less than the second intensity;

acquiring optical detection information corresponding to the light with the first intensity and/or the light with the second intensity;

and analyzing the detection result of the sample according to optical detection information corresponding to the light with the first intensity and/or the light with the second intensity.
The method of claim 17, further comprising performing interferent detection on the sample to obtain interferent detection information for the sample prior to transporting the first container to the first type of test location and/or the second type of test location, the interferent detection information indicating whether an interferent level of the sample exceeds a threshold; said transporting the first container to the first type of test location and/or the second type of test location comprises:

transporting the first container to the first type of location if the interferent content of the sample does not exceed a threshold; or

Transporting the first container to the second type of measurement location if the interferent content of the sample exceeds a threshold.
The method of claim 18, wherein performing interferent detection on the sample to obtain interferent detection information for the sample comprises:

dispensing a portion of the sample and a diluent into a second container;

illuminating the second container with light of a first intensity;

receiving an output optical signal after illuminating the second receptacle;

converting the optical signal into an electrical signal;

and analyzing the electric signal to obtain the detection information of the interferent of the sample.
The method of claim 17, further comprising obtaining interferent detection information for the assay sample at the first type of assay location after transporting the first container to the first type of assay location, the interferent detection information indicating whether an interferent content of the sample exceeds a threshold; said transporting the first container to the first type of test location and/or the second type of test location comprises:

transporting the first container from the first type of measurement location to the second type of measurement location if the interferent content of the sample exceeds a threshold.
The method of claim 17, wherein said transporting the first container to the first type of test location and/or the second type of test location comprises:

transporting the first container to the first type of assay location for optical assay; and

transporting the first container to the second type of assay location for optical assay.
The method of claim 21, wherein analyzing the detection of the sample based on the optical detection information measured at the first type of assay site and/or the second type of assay site comprises: selecting optical detection information measured at the first type measurement position or the second type measurement position according to the detection information of the interferent of the sample; and analyzing the detection result of the sample according to the selected optical detection information.
The method of claim 22, further comprising obtaining interferent detection information for the sample, the interferent detection information indicating whether an interferent content of the sample exceeds a threshold; the selecting optical detection information measured at the first type of assay location or the second type of assay location based on the interferent detection information for the sample comprises:

selecting optical detection information measured by the first type of measurement location if the interferent content of the sample does not exceed a threshold;

selecting optical detection information measured by the second type of measurement location if the interferent content of the sample exceeds a threshold.