CN106399075B - A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror - Google Patents
A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror Download PDFInfo
- Publication number
- CN106399075B CN106399075B CN201610747050.5A CN201610747050A CN106399075B CN 106399075 B CN106399075 B CN 106399075B CN 201610747050 A CN201610747050 A CN 201610747050A CN 106399075 B CN106399075 B CN 106399075B
- Authority
- CN
- China
- Prior art keywords
- reflecting mirror
- optical fiber
- detection system
- fluorescent pcr
- quantitative fluorescent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of detection systems of quantitative fluorescent PCR based on reflecting mirror, including reflecting mirror (1), sample tube frame module (3), photodetector (4), light source (5), optical fiber (6), sample tube frame module (3) is built-in for containing the test tube (2) of sample, the reflecting mirror (1) is also arranged in sample tube frame module (3), reflecting mirror (1) is in the first side of test tube (2), optical fiber (6) are arranged in second side of test tube (2), optical fiber (6) has three ends, wherein, first end is towards sample tube (2) and in the opposite side of reflecting mirror (1), second end couples photodetector (4), third end couples light source (5).The present invention is based on the detection systems of the quantitative fluorescent PCR of reflecting mirror to have the advantages that structure is simple and compact, it is strong to collect fluorescence capability.
Description
Technical field
The invention belongs to biology and technical field of medical detection, and in particular to a kind of compact-sized based on the glimmering of reflecting mirror
The detection system of Fluorescent Quantitative PCR.
Background technique
Fluorescent quantitative PCR technique can make DNA that denaturation, annealing and polymerization extension three be rapidly completed in the very short time
Process, to realize the quick multiplication of DNA quantity.Fluorophor is added in the reaction process of PCR, then uses different-waveband
Light irradiating sample carry out the dyestuff of excited donor and issue fluorescence, collected fluorescence signal is converted into electricity by photodetector
Signal, and handle in a computer, to complete the quantitative analysis to DNA fluorescence, this technology is to biology and medical domain
Research have great importance.Current technology means are by dichroic plane mirror, fluorescence by the way of collecting fluorescence
Focus lens group etc. realizes that entire fluorescent collecting Optical devices need at least 3 eyeglasses.The phosphor collection device of this class formation
Structure is complicated, occupies bulky；If carrying out quantitative fluorescence analysis to multiple samples, optical system can be made more complicated, this
The integrated fluorescent quantitative detection device of sample will occupy more volume, cause very inconvenient in use, and collect fluorescence
The ability of signal is weaker.
Summary of the invention
To solve the above problems existing in the prior art, it is simple and compact based on reflection that the present invention provides a kind of structures
The fluorescence quantitative PCR detection system of mirror.
In order to achieve the above objectives, the present invention adopts the following technical scheme that: a kind of inspection of the quantitative fluorescent PCR based on reflecting mirror
Examining system, including reflecting mirror (1), sample tube frame module (3), photodetector (4), light source (5), optical fiber (6), sample tube
Frame module (3) is built-in for containing the test tube (2) of sample, and sample tube frame module (3) is interior to be also arranged the reflecting mirror (1),
Reflecting mirror (1) is in the first side of test tube (2), and optical fiber (6) are arranged in second side of test tube (2), and optical fiber (6) has three ends, wherein
For first end towards sample tube (2) and in the opposite side of reflecting mirror (1), second end couples photodetector (4), third end connection
Connect light source (5).
The detection system of the quantitative fluorescent PCR based on reflecting mirror, test tube (2) be equipped with it is multiple, it is corresponding, instead
It penetrates mirror (1) and also is provided with muti-piece, the two, which corresponds, to be laid.
The detection system of the quantitative fluorescent PCR based on reflecting mirror, reflecting mirror (1) is wholly transmissive to exciting light, to glimmering
Light is fully reflective.
The detection system of the quantitative fluorescent PCR based on reflecting mirror, the mirror surface of reflecting mirror (1) are in concave shape.
The quantity of the detection system of the quantitative fluorescent PCR based on reflecting mirror, optical fiber (6) can be one or more
Can be corresponding with the quantity of the test tube, optical fiber and test tube correspond, and each optical fiber (6) is respectively installed on a structure of fiber_optic
(7) on.
The detection system of the quantitative fluorescent PCR based on reflecting mirror, the reflection that optical fiber (6) is placed in reflecting mirror (1) are burnt
Point position.
The detection system of the quantitative fluorescent PCR based on reflecting mirror, when optical fiber (6) is arranged one, optical fiber (6) is installed on
On one structure of fiber_optic (7)；Second side of test tube (2) is laid with transmission belt (9), and the both ends of transmission belt (9) are respectively cooperating with one
Root live-roller a, wherein live-roller is connected with the output shaft of motor (8)；The fixed structure of fiber_optic of transmission belt (9)
(7), optical fiber (6) is sent to by position corresponding with each test tube (2) by transmission belt (9), and is in corresponding reflection
The reflector focus position of mirror (1).
The detection system of the quantitative fluorescent PCR based on reflecting mirror, optical fiber (6) use y-type optical fiber.
The detection system of the quantitative fluorescent PCR based on reflecting mirror, light source (5) select laser, xenon lamp or LED light.
The detection system of the quantitative fluorescent PCR based on reflecting mirror, photodetector (4) select photodiode, light
Electric multiplier tube and ccd image sensor.
In the present invention, exciting light is exported by LED or laser, and is coupled into y-type optical fiber and is transmitted.Exciting light irradiation
Test tube equipped with sample, so that sample be excited to generate fluorescence, fluorescence and exciting light are placed on the reflecting mirror collection after test tube, swash
It shines and is transmitted by reflecting mirror, fluorescence is reflected by a reflector and focuses in y-type optical fiber；It is glimmering that y-type optical fiber is placed on reflecting mirror team
The focal point of the reflection of light, to ensure that light as much as possible is transferred to acquisition system by y-type optical fiber collection.Structure of the invention
It is compact, and improve the performance that y-type optical fiber absorbs fluorescence.
Compared with present technology, the present invention is based on the detection systems of the quantitative fluorescent PCR of reflecting mirror to imitate with following technology
Fruit:
1, present invention employs integrated reflecting mirrors, and each individually reflecting mirror can play filtering simultaneously and light is poly-
Burnt effect, so that entire light path system is simple and compact for structure, reduces multi-disc eyeglass and light present in traditional technology
System complex caused by road is lengthy and jumbled and huge problem.
2, the reflecting mirror that the present invention uses has both two kinds of functions of focusing and light splitting, substantially increases system and collects fluorescence signal
Ability.
Detailed description of the invention
Fig. 1 is the top view of the embodiment of the present invention 1.
Fig. 2 is the main view of the embodiment of the present invention 1.
Fig. 3 is the right view of the embodiment of the present invention 1.
Fig. 4 is the structural schematic diagram of the embodiment of the present invention 2.
Specific embodiment
It elaborates in conjunction with attached drawing to the preferred embodiment of the present invention.
Embodiment 1
As shown in Figure 1-3, the detection system of quantitative fluorescent PCR of the present embodiment based on reflecting mirror includes reflecting mirror 1, sample
Test tube 2, sample tube frame module 3, photodetector 4, light source 5, y-type optical fiber 6, y-type optical fiber bracket 7, motor 8, transmission belt
9, eight vertical sample tubes 2 built in sample tube frame module 3 contain sample in sample tube 2.Sample tube frame module 3
Eight pieces of reflecting mirrors 1 are inside also arranged, reflecting mirror 1 is arc-shaped, and eight pieces of reflecting mirrors 1 and eight sample tubes 2 correspond, and vertically sets
In the side of sample tube 2.
The other side of sample tube 2 is laid with transmission belt 9, and the both ends of transmission belt 9 are respectively cooperating with a live-roller,
In a live-roller be connected with the output shaft of motor 8, driven by motor 8 and rotated, so drive transmission belt 9 be driven.
A y-type optical fiber bracket 7 is fixed on transmission belt 9, a y-type optical fiber 6 is fixed on the structure of fiber_optic 7, and y-type optical fiber 6 has
There are three ends, wherein one end face sample tube 2, another both ends are connected with photodetector 4, light source 5 respectively.
The exciting light that light source 5 generates is through the transmission of y-type optical fiber 6 and irradiating sample test tube 2 (part containing sample), to obtain
It is excited to fluorophor and generates fluorescence, exciting light and fluorescence are collected by reflecting mirror 1 simultaneously.Exciting light through reflecting mirror 1 and
Transmission, fluorescence are reflected and focused in y-type optical fiber by reflecting mirrors 1, and then fluorescence is transferred into photodetector by y-type optical fiber
4 are converted into electric signal, and electric signal is finally obtained the fluorescence parameter of the sample of test by the processing of computer.
Light source 5 can select the light sources such as laser, xenon lamp or LED light, and exciting light is issued and is coupled by light source 5
It is transmitted into y-type optical fiber 6.
The quantity and sample tube 2 of reflecting mirror 1 are one-to-one, and are placed on 2 side of sample tube.Reflecting mirror 1 is right
Exciting light is wholly transmissive, fully reflective to fluorescence, and the mirror surface of the reflecting mirror of reflected fluorescent light is concave surface.
In the present embodiment, reflecting mirror by conventional solution reflecting mirror and dichroscope be integrated into an entirety, and
And the quantity of reflecting mirror and the quantity of sample tube are correspondingly, reflecting mirror to be placed in the position opposite with y-type optical fiber.
The plating film type of reflecting mirror be it is wholly transmissive to exciting light, it is fully reflective to fluorescence, facilitate acquisition to enable fluorescence to focus,
The mirror surface of reflected fluorescent light is concave surface.
Y-type optical fiber 6 is placed on the reflector focus position of reflecting mirror 1, so that in fluorescent foci to optical fiber as much as possible, it is real
Now fluorescence is maximumlly collected.
Y-type optical fiber 6 is installed on y-type optical fiber bracket 7, and y-type optical fiber 6 is fixed by y-type optical fiber bracket 7, and y-type optical fiber bracket 7 is solid
Dingan County is placed on transmission belt 9, and is moved under common driving effect of the motor 8 with transmission belt 9, and sample is successively rapidly detected
The fluorescence parameter of sample in product test tube 2.
Photodetector 4 selects the bare headed conversion sensor such as photodiode, photomultiplier tube and ccd image sensor.
Exciting light is optically coupled into y-type optical fiber and transmits wherein from the light sources such as laser or LED, excitation,
Exciting light enters in sample tube from y-type optical fiber outgoing, and wherein the fluorophor of sample is excited and generates fluorescence, excitation
Light and fluorescence are collected by reflecting mirror simultaneously.It is high to fluorescence anti-since reflecting mirror plating film type is high thoroughly to exciting light, so exciting
Light transmits away by reflecting mirror, and fluorescence is reflected by a reflector.In addition, reflecting mirror is concave surface, Y type light to the reflecting surface of fluorescence
Focal point of the fibre just at this reflective concave surface.Fluorescent foci is coupled into y-type optical fiber, is migrated into y-type optical fiber
Photodetector, photodetector can be the shaven head conversion sensing such as photodiode, photomultiplier tube and ccd image sensor
Device, by optical signal converted electrical number, electric signal has finally obtained the fluorescence parameter of the sample of test by the processing of computer.When
In a certain sample tube after sample detection, the drive of y-type optical fiber and its bracket and detection system in motor and transmission belt
Movement is moved to another sample tube under, detects to it, the detection of last completion all samples test tube.
Embodiment 2
In conjunction with Fig. 4, in the present embodiment, y-type optical fiber 6 has eight groups, each y-type optical fiber 6 consistent with the quantity of sample tube
Corresponding to a sample tube 2, and each y-type optical fiber is respectively installed on a y-type optical fiber bracket 7.
By in this present embodiment, each sample tube is correspondingly arranged a y-type optical fiber, therefore, y-type optical fiber is without moving
It is dynamic, the detection of all samples test tube can be completed.Therefore, the present embodiment is without the components such as motor and transmission belt.
In the present embodiment, exciting light comes from laser or LED light source, and excitation is optically coupled into corresponding y-type optical fiber
And transmit wherein, exciting light from corresponding y-type optical fiber outgoing enter in sample tube, wherein the fluorophor of sample by
Fluorescence is generated to excitation, exciting light and fluorescence are collected by reflecting mirror simultaneously.Since reflecting mirror plating film type is to exciting light height
Thoroughly, high to fluorescence anti-, so exciting light transmits away by reflecting mirror, and fluorescence is reflected by a reflector.In addition, reflecting mirror is to glimmering
The reflecting surface of light is concave surface, focal point of the y-type optical fiber just at corresponding reflective concave surface.Fluorescent foci is coupled into Y type
In optical fiber, photodetector is migrated into y-type optical fiber, photodetector can be with photodiode, photomultiplier tube and CCD
The shaven head conversion sensor such as imaging sensor, by optical signal converted electrical number, electric signal is finally obtained by the processing of computer
The fluorescence parameter of the sample of test.In the present embodiment, since y-type optical fiber and its bracket and photoelectric detecting system and sample try
The quantity of pipe is one-to-one, it is therefore not necessary to which mobile y-type optical fiber, can be completed the detection of all samples test tube.
The other contents of the present embodiment can refer to embodiment 1.
The spy that fluorescence quantitative PCR detection device of the present invention has structure simple and compact relative to existing detection device
Point, and can disposably detect all samples.
Those skilled in the art it should be appreciated that the present invention is not limited to the above embodiments, above-described embodiment and
Only illustrate the principle of the invention described in specification, without departing from the spirit and scope of the present invention, the present invention can also
There are various changes and modifications, these changes and improvements all fall within the protetion scope of the claimed invention.
Claims (7)
1. a kind of detection system of the quantitative fluorescent PCR based on reflecting mirror, it is characterized in that including reflecting mirror (1), sample tube frame
Module (3), photodetector (4), light source (5), optical fiber (6), sample tube frame module (3) are built-in for containing the test tube of sample
(2), the reflecting mirror (1) is also arranged in sample tube frame module (3), reflecting mirror (1) is in the first side of test tube (2), tries
Optical fiber (6) are arranged in the second side for managing (2), and optical fiber (6) has three ends, wherein first end is towards sample tube (2) and in reflection
The opposite side of mirror (1), second end couple photodetector (4), and third end couples light source (5)；
The reflecting mirror (1) is wholly transmissive to exciting light, fully reflective to fluorescence；
The optical fiber (6) is arranged one, and optical fiber (6) is installed on a structure of fiber_optic (7)；Second side of test tube (2) is laid with
Transmission belt (9), the both ends of transmission belt (9) are respectively cooperating with a live-roller, wherein the output of a live-roller and motor (8)
Axis is connected；Transmission belt (9) structure of fiber_optic (7), by transmission belt (9) by optical fiber (6) be sent to it is each
The corresponding position of test tube (2), and it is in the reflector focus position of corresponding reflecting mirror (1).
2. the detection system of the quantitative fluorescent PCR based on reflecting mirror as described in claim 1, it is characterized in that: the test tube (2)
Corresponding equipped with multiple, reflecting mirror (1) also is provided with muti-piece, and the two, which corresponds, to be laid.
3. the detection system of the quantitative fluorescent PCR based on reflecting mirror as described in claim 1, it is characterized in that: the reflecting mirror (1)
Mirror surface be in concave shape.
4. the detection system of the quantitative fluorescent PCR based on reflecting mirror as described in claim 1, it is characterized in that: the optical fiber (6)
It is placed in the reflector focus position of reflecting mirror (1).
5. the detection system of the quantitative fluorescent PCR based on reflecting mirror as described in claim 1, it is characterized in that: the optical fiber (6)
Using y-type optical fiber.
6. the detection system of the quantitative fluorescent PCR based on reflecting mirror as described in claim 1, it is characterized in that: the light source (5)
Select laser, xenon lamp or LED light.
7. the detection system of the quantitative fluorescent PCR based on reflecting mirror as described in claim 1, it is characterized in that: the photoelectricity is visited
It surveys device (4) and selects photodiode, photomultiplier tube and ccd image sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610747050.5A CN106399075B (en) | 2016-08-26 | 2016-08-26 | A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610747050.5A CN106399075B (en) | 2016-08-26 | 2016-08-26 | A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106399075A CN106399075A (en) | 2017-02-15 |
| CN106399075B true CN106399075B (en) | 2019-05-03 |
Family
ID=58003139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610747050.5A Active CN106399075B (en) | 2016-08-26 | 2016-08-26 | A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106399075B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113791059A (en) * | 2021-09-16 | 2021-12-14 | 大连理工大学 | Capillary fluorometer with low background signal |
| CN115747039A (en) * | 2022-11-02 | 2023-03-07 | 苏州中科医疗器械产业发展有限公司 | Full-automatic integrated high-flux digital nucleic acid detection system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8264687B2 (en) * | 2006-02-13 | 2012-09-11 | Pacific Biosciences Of California, Inc. | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
| CN206109400U (en) * | 2016-08-26 | 2017-04-19 | 浙江科技学院 | Fluorescent quantitation PCR's detecting system based on speculum |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101464409B (en) * | 2007-12-19 | 2013-10-30 | 中国科学院电子学研究所 | Apparatus and method for fast quantitative bacteria detection |
| CN201553741U (en) * | 2009-11-16 | 2010-08-18 | 杭州博日科技有限公司 | Multiwavelength fluorescence detection device of quantitative PCR |
| CN204462019U (en) * | 2015-02-06 | 2015-07-08 | 余家昌 | A kind of subminiaturization hyperchannel real-time fluorescence spectrum detection device |
-
2016
- 2016-08-26 CN CN201610747050.5A patent/CN106399075B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8264687B2 (en) * | 2006-02-13 | 2012-09-11 | Pacific Biosciences Of California, Inc. | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
| CN206109400U (en) * | 2016-08-26 | 2017-04-19 | 浙江科技学院 | Fluorescent quantitation PCR's detecting system based on speculum |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106399075A (en) | 2017-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202057580U (en) | Optical system for fluorescent detection of a photo-conductive relay (PCR) amplifier with quantitative property | |
| CN102033058B (en) | Super resolution fluorescence lifetime imaging system | |
| CN103487421B (en) | Time gate wide field stimulated radiation super-resolution microscopic method and device | |
| CN205539686U (en) | Burnt spectromicroscope of small -size scanning copolymerization | |
| CN105300943B (en) | A kind of microscope integrated optical circuit system for drop fluorescence detection | |
| CN104535481B (en) | imaging flow cytometer | |
| JP6513802B2 (en) | Laser light coupling for nanoparticle detection | |
| US8633432B2 (en) | Reflective focusing and transmissive projection device | |
| CN109828364B (en) | Vacuum intracavity confocal microscopic imaging system and method based on cage structure | |
| WO2004106903A1 (en) | Light receiving unit and measuring device comprising it | |
| CN103940796A (en) | Novel multi-angle and multi-mode quick switching circular optical illumination microscopic imaging system | |
| CN106399075B (en) | A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror | |
| US20130321814A1 (en) | Systems and methods for screening of biological samples | |
| CN206109400U (en) | Fluorescent quantitation PCR's detecting system based on speculum | |
| CN103901009B (en) | A kind of laser-induced fluorescence detection system of optical fiber scan type | |
| CN203479709U (en) | Rotational scan type multi-channel capillary electrophoresis chip laser-induction fluorescence detection system | |
| CN203231972U (en) | Photoluminescence imaging detection equipment for high-speed line scanning of solar silicon wafers | |
| CN105388131A (en) | Fluorescence detection instrument and system based on micro-fluidic chip | |
| CN103033464A (en) | Photoacoustic-fluorescence flow cytometer | |
| CN104792754A (en) | Detection device and method adopting lased-induced liquid fluorescence | |
| CN105866075A (en) | Fluorescence detection apparatus | |
| US20080030721A1 (en) | Optical apparatus and methods for chemical analysis | |
| WO2010054524A1 (en) | Method and device for non-contact and fixed fluorescence molecular tomography | |
| CN2551994Y (en) | Scanner for biogene chip | |
| CN101324527A (en) | Device and method for scanning total reflection type laser induced fluorescent common focusing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |