CN104792754A - Detection device and method adopting lased-induced liquid fluorescence - Google Patents
Detection device and method adopting lased-induced liquid fluorescence Download PDFInfo
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- CN104792754A CN104792754A CN201510165613.5A CN201510165613A CN104792754A CN 104792754 A CN104792754 A CN 104792754A CN 201510165613 A CN201510165613 A CN 201510165613A CN 104792754 A CN104792754 A CN 104792754A
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Abstract
The invention discloses a detection device adopting lased-induced liquid fluorescence and relates to the technical field of fluorescence spectra. The detection device comprises an automatic injected sample cleaning system, a fluorescence excitation system and a beam splitting system, wherein the automatic injected sample cleaning system is located in the middle of the whole device, and the fluorescence excitation system and the beam splitting system are located on two sides of the automatic injected sample cleaning system respectively. A liquid-core optical fiber is filled with a to-be-measured liquid, fluorescence produced by excitation is transmitted in the liquid-core optical fiber along with incident light and accumulated and intensified continuously, a laser is all limited in the whole liquid-core optical fiber to be transmitted after coupled to enter the liquid-core optical fiber, exciting light interacts with the to-be-detected liquid continuously, the total effective exciting light path is extended, and the intensity of the fluorescence produced by excitation is accumulated continuously. The liquid-core optical fiber can be very long, so that the intensity of the fluorescence can be increased substantially in the optical fiber, and fluorescence detection of low-concentration substances can be realized.
Description
Technical field
The present invention relates to fluorescence spectroscopy technique field, refer in particular to a kind of highly sensitive fluorescent liquid pick-up unit.
Background technology
Fluorescence is a kind of photoluminescence phenomenon, when material is after the incident light (normally visible ray, ultraviolet or X ray) of certain specific wavelength irradiates, absorb after luminous energy and enter excited state, and exit excited state immediately and send the emergent light longer than the wavelength of incident light.And incident light is once stop, luminescence phenomenon also disappears thereupon immediately.The emergent light with this character is called fluorescence.
Fluorescence spectrophotometer comprises three basic modules: light source, liquid sample pool, detector, and wherein light source can use the light source of single wavelength, may also be the continuously adjustable monochromator of wavelength; Liquid sample pool is generally cuvette, and detector often adopts photomultiplier or CCD.
When utilizing fluorescence spectrophotometer to detect determinand, the fluorescence of determinand need obtain mostly under solution state, and solvent adopts the unstressed configuration such as water or deionized water solvent mostly.The liquid sample pool adopted commonly uses the rectangular parallelepiped transparent vessel be made up of quartz material.During test, fill the liquid of determinand in quartz container, exciting light is through a certain side of quartz container, and be irradiated on solution to be measured, the fluorescence exciting generation is collected in the another side perpendicular in the incident side with quartz container by lens.As patent 200820075445.6, name are called that " laser Raman/fluorescent spectrometer " adopts sample QC to hold sample, patent 103234950A, name are called, and " parallel double light path laser induced fluorescence spectrometer " adopts capillary column to hold sample.The volume of these two kinds of liquid sample pools is all less, and can only excite and collect the fluorescence of condenser lens near focal point, and fluorescence excitation, apart from short, excite the fluorescence intensity of generation low, causes sensitivity lower, not high to the accuracy of detection of low concentration sample fluorescence.Fan Yingfeng (assay office, 05 phase in 2008, p118-122) a kind of laser-Induced Fluorescence Detection device is disclosed, this device adopts low-refraction teflon hollow tubular and circulating water solution to form liquid-core optical fibre, laser vertical is irradiated on liquid-core optical fibre, utilizes CCD detecting device collect at the other end of liquid-core optical fibre and detect the fluorescence exciting generation.This device only has the solution in zonule to be excited generation fluorescence too, fails to make full use of the object that liquid-core optical fibre realizes long distance fluorescence excitation.Method disclosed in these, to only have compared with small part solution to be measured by laser excitation, therefore can only produce a small amount of fluorescence.Patent 201110343868.8, name are called that " a kind of liquid core waveguide fluorescence testing apparatus " utilizes large area exciting light sources to be irradiated to the length of whole liquid-core optical fibre, all liq in optical fiber can be produced fluorescence by laser excitation, but when adopting area source as excitation source, cause excitating light strength more weak because exciting light energy can not concentrate, be difficult to the fluorescence exciting generation stronger, be also unfavorable for the fluoroscopic examination of low concentration solution.
Summary of the invention
The object of the invention is for overcoming the deficiencies in the prior art, proposing a kind of pick-up unit adopting induced with laser fluorescent liquid.Testing liquid sample is filled with in liquid-core optical fibre by this device, exciting light excites testing liquid in the length of whole liquid-core optical fibre, add the fluorescence excitation distance of liquid sample to be measured, fluorescence constantly accumulates enhancing in whole liquid-core optical fibre, thus substantially increase the fluorescence intensity of testing liquid sample, the fluoroscopic examination to low-concentration liquid sample can be realized.
Technical scheme of the present invention is:
A pick-up unit for induced with laser fluorescent liquid, comprises automatic sampling cleaning system, fluorescence excitation system, beam splitting system; Wherein automatic sampling cleaning system is positioned in the middle of whole device, and fluorescence excitation system and beam splitting system lay respectively at the both sides of automatic sample handling system.
Described automatic sampling cleaning system comprises liquid sample pool, vacuum pump, kapillary I, liquid-core optical fibre adapter I, fiber clamp I, liquid-core optical fibre, fiber clamp II, liquid-core optical fibre adapter II, kapillary II; Wherein liquid-core optical fibre is fixed by fiber clamp I and fiber clamp II; The two ends of liquid-core optical fibre are by liquid-core optical fibre adapter I and liquid-core optical fibre adapter II compact siro spinning technology kapillary I and kapillary II respectively, and kapillary I is connected with liquid sample pool, and kapillary II is connected with vacuum pump.
Wherein said liquid-core optical fibre adapter I and liquid-core optical fibre adapter II comprises an elbow be made up of quartz material, hollow rubber pipe I, hollow rubber pipe II and a slice quartz-optical glass pane.Be processed into the circular hole of two mutual square crossings in the elbow that quartz material is made, wherein circular hole both ends of the surface are got through, and another a circular hole only end face is got through.The one side of circular hole place end face and hollow rubber pipe I tight bond (hollow rubber bore I is identical with liquid-core optical fibre external diameter) are got through in two ends, and other end is together with quartz-optical glass pane tight bond.Laser is entered in liquid-core optical fibre by quartz-optical glass pane.Only an end face gets through the circular hole end face at circular hole place and hollow rubber pipe II tight bond (hollow rubber pipe II internal diameter is identical with kapillary II external diameter with kapillary I).
Described fluorescence excitation system is used for exciting light to be coupled in liquid-core optical fibre to excite generation fluorescence.Comprise laser instrument (laser power is adjustable), completely reflecting mirror, fiber coupling lens, liquid-core optical fibre adapter I, liquid-core optical fibre.The angular separation of completely reflecting mirror and laser emitting is 45 °, and fiber coupling lens is placed on return laser beam direction, and the center of laser instrument, completely reflecting mirror, fiber coupling lens and liquid-core optical fibre adapter I is on same axis.Laser sequentially passes through completely reflecting mirror and turns back after 90 °, then assembles through fiber coupling lens and enter in liquid-core optical fibre.Liquid-core optical fibre is located by liquid-core optical fibre adapter I and liquid-core optical fibre adapter II, and liquid-core optical fibre adapter I is fixed on fiber clamp I, and liquid-core optical fibre adapter II is fixed on fiber clamp II.Laser is limited in transmission in whole liquid-core optical fibre, and exciting light and fluid to be measured constantly act on, and extend and always effectively excite light path, excite the fluorescence signal intensity of generation constantly to be accumulated, thus substantially increase the fluorescence intensity of testing liquid.
Described beam splitting system, for collecting the fluorescence from the outgoing of the liquid-core optical fibre other end, comprises fiber clamp III, silica fibre, optical fiber interface, slit, non-spherical reflector, spherical lens grating, line array CCD, signal processing module, computing machine composition.Silica fibre is fixed on fiber clamp III, and silica fibre one end is near the quartz-optical center of glass of liquid-core optical fibre joint II, and the other end is connected with optical fiber interface, and fluorescence is imported slit in liquid-core optical fibre.Slit faces non-spherical reflector, and non-spherical reflector inwall is coated with total reflection film, and fluorescence, by being incident to non-spherical reflector after slit, is collimated into parallel beam by non-spherical reflector.A spherical lens grating is had, after the fluorescence being collimated into parallel beam is incident to spherical lens grating, by spherical lens optical grating reflection and dispersion becomes numerous parallel beam in the middle of slit and non-spherical reflector.Numerous parallel beam is incident on same non-spherical reflector, the line array CCD being focused to non-spherical lens front by non-spherical reflector produces simulating signal, simulating signal after signal processing module and computer disposal, by Spectrum software record fluorescence spectrum.
Utilize above-mentioned induced with laser fluorescent liquid pick-up unit to obtain the fluorescence spectrum method of liquid substance, carry out according to following step:
(1) insertion of kapillary I one end is equipped with in the liquid sample pool of testing liquid, other end connecting fluid core fibre adapter I; Kapillary II one end connects vacuum pump, other end connecting fluid core fibre adapter II.
(2) open vacuum pump, in sample cell, testing liquid is sucked in liquid-core optical fibre pipe forms liquid-core optical fibre through kapillary I, liquid-core optical fibre adapter I.
(3) open laser instrument, the laser beam that laser instrument sends is rolled over by completely reflecting mirror and is turn 90 degrees, and the laser beam after turning back is entered in liquid-core optical fibre by the quartz-optical glass pane end coupling of fiber coupling lens through liquid-core optical fibre adapter I.Now, laser is limited in transmission in liquid-core optical fibre, in liquid-core optical fibre length range constantly and testing liquid effect excite generation fluorescence.The fluorescence of generation major part is excited to penetrate from the other end of liquid-core optical fibre through the quartz-optical glass pane of liquid-core optical fibre adapter II.Silica fibre one end is close on the quartz-optical glass pane of liquid-core optical fibre adapter II, other end connecting fiber interface, and the fluorescence penetrated from liquid-core optical fibre adapter II is transferred to slit.After non-spherical reflector reflection, parallel beam is become by the fluorescence of slit, parallel beam dispersion after spherical lens optical grating reflection becomes the parallel beam of different wave length, parallel beam after dispersion is through focusing on line array CCD with a slice non-spherical reflector, produce simulating signal, simulating signal after signal processing module and computer disposal, by Spectrum software record fluorescence spectrum.
Beneficial effect of the present invention:
1. testing liquid is full of liquid-core optical fibre, the fluorescence of generation is excited to transmit in liquid-core optical fibre with incident light, and constantly strengthened by accumulation, after laser is coupled into liquid-core optical fibre, all be limited in transmission in whole liquid-core optical fibre, exciting light and fluid to be measured constantly act on, and extend and always effectively excite light path, excite the fluorescence intensity of generation constantly to be accumulated.Because liquid-core optical fibre can be very long, thus fluorescence intensity can significantly be improved in optical fiber, can realize the fluoroscopic examination of low concentration material.
2. laser power is adjustable, by regulating the power of laser, the laser intensity of liquid-core optical fibre exit end can be made to reduce, and even close to zero, does not need to use expensive fluorescent optical filter to carry out filtering exciting light, reduces costs.
3. light-dividing device only uses a slice non-spherical reflector and a slice lenticulation, easy to adjust, reduces costs simultaneously.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation of induced with laser fluorescent liquid pick-up unit, in figure, 1 is laser instrument, 2 is completely reflecting mirror, 3 is fiber coupling lens, 4 is liquid sample pool, 5 is kapillary I, 6 is liquid-core optical fibre adapter I, 7 is fiber clamp I, 8 is liquid-core optical fibre, 9 is fiber clamp II, 10 is liquid-core optical fibre adapter II, 11 is kapillary II, 12 is vacuum pump, 13 is fiber clamp III, 14 is silica fibre, 15 is optical fiber interface, 16 is slit, 17 is non-spherical reflector, 18 is spherical lens grating, 19 is line array CCD, 20 is signal processing module, 21 is computing machine,
Fig. 2 is liquid-core optical fibre schematic diagram, and in figure, 22 is teflon hollow tubular, and 23 is solution to be measured, 24 light for being coupled into;
Fig. 3 is liquid-core optical fibre adapter structure figure, and in figure, 25 is hollow rubber pipe I, 26 elbows made for quartz material, and 27 is hollow rubber pipe II, and 28 is quartz-optical glass pane;
Fig. 4 is the sectional view of liquid-core optical fibre adapter structure figure, and in figure, 25 is hollow rubber pipe I, 26 elbows made for quartz material, and 27 is hollow rubber pipe II, and 28 is quartz-optical glass pane;
Fig. 5 is light-dividing device schematic diagram, and in figure, 15 is optical fiber interface, and 16 is slit, and 17 is non-spherical reflector, and 18 is spherical lens grating, and 19 is line array CCD, and 20 is signal processing module;
Fig. 6 is a kind of olive oil fluorescence spectrum comparison diagram using the induced with laser fluorescent liquid spectrum detection device of liquid-core optical fibre and do not use the general fluorescenes spectrum pick-up unit of liquid-core optical fibre to obtain of the present invention;
Embodiment
Below in conjunction with accompanying drawing, the present invention is further described.A pick-up unit for induced with laser fluorescent liquid, comprises automatic sampling cleaning system, fluorescence excitation system, beam splitting system; Wherein automatic sampling cleaning system is positioned in the middle of whole device, and fluorescence excitation system and beam splitting system lay respectively at the both sides of automatic sample handling system.
Described automatic sampling cleaning system comprises liquid sample pool 4, vacuum pump 12, kapillary I 5, liquid-core optical fibre adapter I 6, fiber clamp I 7, liquid-core optical fibre 8, fiber clamp II 9, liquid-core optical fibre adapter II 10, kapillary II 11; Wherein liquid-core optical fibre 8 is fixed by fiber clamp I 7 and fiber clamp II 9; The two ends of liquid-core optical fibre 8 are by liquid-core optical fibre adapter I 6 and liquid-core optical fibre adapter II 10 compact siro spinning technology kapillary I 5 and kapillary II 11 respectively, and kapillary I 5 is connected with liquid sample pool 4, and kapillary II 11 is connected with vacuum pump 12.
Wherein said liquid-core optical fibre adapter I 6 and liquid-core optical fibre adapter II 10 comprises an elbow be made up of quartz material 26, hollow rubber pipe I 25, hollow rubber pipe II 27 and a slice quartz-optical glass pane 28.Be processed into the circular hole of two mutual square crossings in the elbow 26 that quartz material is made, wherein circular hole both ends of the surface are got through, and another a circular hole only end face is got through.The one side of circular hole place end face and hollow rubber pipe I 25 tight bond (hollow rubber bore I is identical with liquid-core optical fibre external diameter) are got through in two ends, and other end is together with quartz-optical glass pane 28 tight bond.Laser enters liquid-core optical fibre 8 by quartz-optical glass pane 28.Only an end face gets through the circular hole end face at circular hole place and hollow rubber pipe II 27 tight bond (hollow rubber pipe II 27 internal diameter is identical with kapillary II 11 external diameter with kapillary I 5).
Described fluorescence excitation system is used for exciting light to be coupled in liquid-core optical fibre 8, excites generation fluorescence.Comprise laser instrument 1 (laser power is adjustable), completely reflecting mirror 2, fiber coupling lens 3, liquid-core optical fibre adapter I 6, liquid-core optical fibre 8.Completely reflecting mirror 3 is 45 ° with the angular separation of laser instrument 1 emergent light.Fiber coupling lens 3 is placed on return laser beam direction, and the center of laser instrument 1, completely reflecting mirror 2, fiber coupling lens 3 and liquid-core optical fibre adapter I 6 is on same axis.Laser sequentially passes through completely reflecting mirror 2 and turns back after 90 °, then assembles through fiber coupling lens 3 and enter in liquid-core optical fibre 8.Liquid-core optical fibre 8 is located by liquid-core optical fibre adapter I 6 and liquid-core optical fibre adapter II 10, and liquid-core optical fibre adapter I 6 is fixed on fiber clamp I 7, and liquid-core optical fibre adapter II 10 is fixed on fiber clamp II 13.Laser is limited in transmission in whole liquid-core optical fibre 8, and exciting light and fluid to be measured constantly act on, and extend and always effectively excite light path, excite the fluorescence signal intensity of generation constantly to be accumulated, thus substantially increase the fluorescence intensity of testing liquid.
Described beam splitting system, for collecting the fluorescence from the outgoing of liquid-core optical fibre 8 other end, comprises fiber clamp III13, silica fibre 14, optical fiber interface 15, slit 16, non-spherical reflector 17, spherical lens grating 18, line array CCD 19, signal processing module 20, computing machine 21 forms.Silica fibre 14 is fixed on fiber clamp III 13, and silica fibre 14 one end is near quartz-optical center of glass in liquid-core optical fibre joint II 10, and the other end is connected with optical fiber interface 15, and fluorescence is imported slit 16 in liquid-core optical fibre 8.Slit 16 faces non-spherical reflector 17, and non-spherical reflector 17 inwall is coated with total reflection film, and fluorescence, by being incident to non-spherical reflector 17 after slit 16, is collimated into parallel beam by non-spherical reflector 17.There is a spherical lens grating 18 in the middle of slit 16 and non-spherical reflector 17, after the fluorescence being collimated into parallel beam is incident to spherical lens grating 18, reflected by spherical lens grating 18, and dispersion becomes numerous parallel beam.Numerous parallel beam is incident on same non-spherical reflector 17, focused on the line array CCD 19 in non-spherical lens 17 front by non-spherical reflector 17, produce simulating signal, simulating signal after signal processing module 20 and computing machine 21 process, by Spectrum software record fluorescence spectrum.
Liquid-core optical fibre 8 material selection teflon hollow tubular, this material has high-low temperature resistant, corrosion-resistant, strong alkali-acid resistance, the advantages such as friction factor is little, and the refractive index of polytetrafluoroethylmaterial material only has 1.29, is less than the refractive index 1.33 of pure water.The schematic diagram of described liquid-core optical fibre as shown in Figure 2, utilizes polyfluortetraethylene pipe 22 as the covering of liquid-core optical fibre 8, and solution 23 to be measured is as the core diameter of liquid-core optical fibre 8, and solution to be measured can be aqueous solution or other solvent.Now, teflon is optically thinner medium, and aqueous solution or other solvent are optically denser mediums.When being coupled into light angle and being less than the cirtical angle of total reflection, due to total reflection effect, incident light will be limited in transmission in liquid-core optical fibre 8.
If kept flat by liquid-core optical fibre 8, the solution to be measured 24 in liquid-core optical fibre 8 will spill, and therefore need the mode taking to seal or install additional adapter to liquid-core optical fibre 8, to ensure that the solution to be measured 24 in liquid-core optical fibre 8 can not overflow.Because liquid-core optical fibre 8 will repeatedly use, should not sealing mode be taked, design and produce a kind of liquid-core optical fibre adapter I 6, liquid-core optical fibre adapter II 10.Fig. 3 is the structural drawing of liquid-core optical fibre adapter I 6 and liquid-core optical fibre adapter II 10, Fig. 4 is the sectional view of liquid-core optical fibre adapter structure, and elbow 26, hollow rubber pipe 27 and quartz-optical glass pane 28 that liquid-core optical fibre adapter I 6, liquid-core optical fibre adapter II 10 are made up of hollow rubber pipe 25, quartz material form.Have the circular hole of two mutual square crossings in the elbow 26 that quartz material is made, wherein circular hole both ends of the surface are got through, and another a circular hole only end face is got through.The one side that circular hole place end face is got through at two ends is closely glued, together with other end closely bonds with quartz-optical glass pane 28 mutually with hollow rubber pipe I 25 (hollow rubber pipe I internal diameter is identical with liquid-core optical fibre 8 internal diameter).The end face that only an end face gets through circular hole place closely glues mutually with hollow rubber pipe II 27 (hollow rubber pipe II internal diameter is identical with kapillary II external diameter with kapillary I).Liquid-core optical fibre 8 is connected on liquid-core optical fibre adapter 6, because hollow rubber pipe I25 is to the squeezing action of liquid-core optical fibre 8, ensures that in liquid-core optical fibre 8, solution 23 to be measured can not overflow.
Described beam splitting system adopts crossed-symmetrical structure, by the best optical texture obtained after optical software simulative optimization.Fig. 5 is the schematic diagram of beam splitting system, is made up of optical fiber interface 15, slit 16, non-spherical reflector 17, spherical lens grating 18, line array CCD 19, signal processing module 20.The fluorescence of generation is excited to enter from silica fibre 14 through optical fiber interface 15, by inciding on non-spherical reflector 17 after slit 16, incident beam is collimated into parallel beam after non-spherical reflector 17 reflects, parallel beam incident is to spherical lens grating 18 back reflection and dispersion becomes the parallel beam of different wave length, after the parallel beam of different wave length incides non-spherical reflector 17 again, be focused onto on line array CCD 19 and produce simulating signal, simulating signal after signal processing module 20 and computing machine 21 process, by Spectrum software record fluorescence spectrum.Wherein non-spherical reflector 17 is coaxial with spherical lens grating 18, has the simple feature of Installation and Debugging.
Fig. 6 is a kind of olive oil fluorescence normalization spectrum comparison diagram using the induced with laser fluorescent liquid spectrum detection device of liquid-core optical fibre and do not use the general fluorescenes spectrum pick-up unit of liquid-core optical fibre to obtain of the present invention.The olive oil laser-induced fluorescence spectroscopy of curve in figure represented by "---" measured by a kind of pick-up unit using the induced with laser fluorescent liquid of liquid-core optical fibre of the present invention, the curve represented by "-" for do not use liquid-core optical fibre general fluorescenes spectrum pick-up unit measured by olive oil fluorescence spectrum.As seen from Figure 6, the induced with laser fluorescent liquid spectrum detection device of use liquid-core optical fibre of the present invention is surveyed fluorescent characteristics peak and is surveyed abundant than general fluorescenes spectrum pick-up unit.In the olive oil fluorescence spectrum that general fluorescenes spectrum pick-up unit is surveyed, only have 679 places to have spectrum peak, this spectrum peak is chlorophyllous characteristic peak contained by olive oil oil.And in the olive oil fluorescence spectrum measured by pick-up unit of a kind of induced with laser fluorescent liquid of the present invention, 4,590nm, 634nm, 718nm, 780nm place is had to compose peak, wherein 590nm, 634nm compose the characteristic peak that peak is olive oil contained vitamin E, and 718nm, 780nm be chlorophyllous characteristic peak contained by olive oil.Illustrate that the pick-up unit of induced with laser fluorescent liquid of the present invention utilizes liquid-core optical fibre to increase fluorescence excitation length, more fluorescent characteristics peaks information can be obtained, and there is very high sensitivity, can be used for the analysis of low concentration residual fluorescence material.
Claims (6)
1. a pick-up unit for induced with laser fluorescent liquid, is characterized in that comprising automatic sampling cleaning system, fluorescence excitation system, beam splitting system; Wherein automatic sampling cleaning system is positioned in the middle of whole device, and fluorescence excitation system and beam splitting system lay respectively at the both sides of automatic sample handling system.
2. the pick-up unit of a kind of induced with laser fluorescent liquid according to claim 1, is characterized in that described automatic sampling cleaning system comprises liquid sample pool, vacuum pump, kapillary I, liquid-core optical fibre adapter I, fiber clamp I, liquid-core optical fibre, fiber clamp II, liquid-core optical fibre adapter II, kapillary II; Wherein liquid-core optical fibre is fixed by fiber clamp I and fiber clamp II; The two ends of liquid-core optical fibre are by liquid-core optical fibre adapter I and liquid-core optical fibre adapter II compact siro spinning technology kapillary I and kapillary II respectively, and kapillary I is connected with liquid sample pool, and kapillary II is connected with vacuum pump.
3. the pick-up unit of a kind of induced with laser fluorescent liquid according to claim 1, is characterized in that wherein said liquid-core optical fibre adapter I and liquid-core optical fibre adapter II comprises an elbow be made up of quartz material, hollow rubber pipe I, hollow rubber pipe II and a slice quartz-optical glass pane; Be processed into the circular hole of two mutual square crossings in the elbow that quartz material is made, wherein circular hole both ends of the surface are got through, and another a circular hole only end face is got through; The one side of circular hole place end face and hollow rubber pipe I tight bond are got through in two ends, and wherein hollow rubber bore I is identical with liquid-core optical fibre external diameter, and other end is together with quartz-optical glass pane tight bond; Laser enters in liquid-core optical fibre by quartz-optical glass pane; Only an end face gets through circular hole end face and the hollow rubber pipe II tight bond at circular hole place, and namely hollow rubber pipe II internal diameter is identical with kapillary II external diameter with kapillary I.
4. the pick-up unit of a kind of induced with laser fluorescent liquid according to claim 1, is characterized in that described fluorescence excitation system excites generation fluorescence for being coupled in liquid-core optical fibre by exciting light; Comprise laser instrument wherein laser power adjustable, completely reflecting mirror, fiber coupling lens, liquid-core optical fibre adapter I, liquid-core optical fibre; The angular separation of completely reflecting mirror and laser emitting is 45 °, and fiber coupling lens is placed on return laser beam direction, and the center of laser instrument, completely reflecting mirror, fiber coupling lens and liquid-core optical fibre adapter I is on same axis; Laser sequentially passes through completely reflecting mirror and turns back after 90 °, then assembles through fiber coupling lens and enter in liquid-core optical fibre; Liquid-core optical fibre is located by liquid-core optical fibre adapter I and liquid-core optical fibre adapter II, and liquid-core optical fibre adapter I is fixed on fiber clamp I, and liquid-core optical fibre adapter II is fixed on fiber clamp II; Laser is limited in transmission in whole liquid-core optical fibre, and exciting light and fluid to be measured constantly act on, and extend and always effectively excite light path, excite the fluorescence signal intensity of generation constantly to be accumulated, thus substantially increase the fluorescence intensity of testing liquid.
5. the pick-up unit of a kind of induced with laser fluorescent liquid according to claim 1, it is characterized in that described beam splitting system is for collecting the fluorescence from the outgoing of the liquid-core optical fibre other end, comprising fiber clamp III, silica fibre, optical fiber interface, slit, non-spherical reflector, spherical lens grating, line array CCD, signal processing module, computing machine composition; Silica fibre is fixed on fiber clamp III, and silica fibre one end is near the quartz-optical center of glass of liquid-core optical fibre joint II, and the other end is connected with optical fiber interface, and fluorescence is imported slit in liquid-core optical fibre; Slit faces non-spherical reflector, and non-spherical reflector inwall is coated with total reflection film, and fluorescence, by being incident to non-spherical reflector after slit, is collimated into parallel beam by non-spherical reflector; A spherical lens grating is had, after the fluorescence being collimated into parallel beam is incident to spherical lens grating, by spherical lens optical grating reflection and dispersion becomes numerous parallel beam in the middle of slit and non-spherical reflector; Numerous parallel beam is incident on same non-spherical reflector, the line array CCD being focused to non-spherical lens front by non-spherical reflector produces simulating signal, simulating signal after signal processing module and computer disposal, by Spectrum software record fluorescence spectrum.
6. utilize above-mentioned induced with laser fluorescent liquid pick-up unit to obtain the fluorescence spectrum method of liquid substance, it is characterized in that carrying out according to following step:
(1) insertion of kapillary I one end is equipped with in the liquid sample pool of testing liquid, other end connecting fluid core fibre adapter I; Kapillary II one end connects vacuum pump, other end connecting fluid core fibre adapter II;
(2) open vacuum pump, in sample cell, testing liquid is sucked in liquid-core optical fibre pipe forms liquid-core optical fibre through kapillary I, liquid-core optical fibre adapter I;
(3) open laser instrument, the laser beam that laser instrument sends is rolled over by completely reflecting mirror and is turn 90 degrees, and the laser beam after turning back is entered in liquid-core optical fibre by fiber coupling lens through the quartz-optical glass pane end coupling of liquid-core optical fibre adapter I; Now, laser is limited in transmission in liquid-core optical fibre, in liquid-core optical fibre length range constantly and testing liquid effect excite generation fluorescence; The fluorescence of generation major part is excited to penetrate from the other end of liquid-core optical fibre through the quartz-optical glass pane of liquid-core optical fibre adapter II; Silica fibre one end is close on the quartz-optical glass pane of liquid-core optical fibre adapter II, other end connecting fiber interface, and the fluorescence penetrated from liquid-core optical fibre adapter II is transferred to slit; After non-spherical reflector reflection, parallel beam is become by the fluorescence of slit, parallel beam dispersion after spherical lens optical grating reflection becomes the parallel beam of different wave length, parallel beam after dispersion is through focusing on line array CCD with a slice non-spherical reflector, produce simulating signal, simulating signal after signal processing module and computer disposal, by Spectrum software record fluorescence spectrum.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353294A (en) * | 2016-11-09 | 2017-01-25 | 武汉能斯特科技有限公司 | Miniature capillary luminoscope |
| CN106442449A (en) * | 2016-10-17 | 2017-02-22 | 中国科学院合肥物质科学研究院 | Laser-induced fluorescence detection device for bioaerosol |
| CN109211839A (en) * | 2018-09-01 | 2019-01-15 | 哈尔滨工程大学 | A kind of binary channels side-hole fiber grating sensing device |
| CN113155795A (en) * | 2021-04-15 | 2021-07-23 | 西北核技术研究所 | Device and method for directly measuring upper energy level fluorescence lifetime of rare earth element doped optical fiber laser |
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2015
- 2015-04-07 CN CN201510165613.5A patent/CN104792754A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442449A (en) * | 2016-10-17 | 2017-02-22 | 中国科学院合肥物质科学研究院 | Laser-induced fluorescence detection device for bioaerosol |
| CN106353294A (en) * | 2016-11-09 | 2017-01-25 | 武汉能斯特科技有限公司 | Miniature capillary luminoscope |
| CN109211839A (en) * | 2018-09-01 | 2019-01-15 | 哈尔滨工程大学 | A kind of binary channels side-hole fiber grating sensing device |
| CN109211839B (en) * | 2018-09-01 | 2021-01-12 | 哈尔滨工程大学 | Double-channel side hole fiber grating sensing device |
| CN113155795A (en) * | 2021-04-15 | 2021-07-23 | 西北核技术研究所 | Device and method for directly measuring upper energy level fluorescence lifetime of rare earth element doped optical fiber laser |
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