CN106399075A - Reflector based fluorescent quantitative PCR detection system - Google Patents
Reflector based fluorescent quantitative PCR detection system Download PDFInfo
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- CN106399075A CN106399075A CN201610747050.5A CN201610747050A CN106399075A CN 106399075 A CN106399075 A CN 106399075A CN 201610747050 A CN201610747050 A CN 201610747050A CN 106399075 A CN106399075 A CN 106399075A
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- reflecting mirror
- optical fiber
- detecting system
- fluorescent pcr
- pcr based
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
The invention discloses a reflector based fluorescent quantitative PCR detection system, which consists of: reflectors (1), a sample test tube rack module (3), a photoelectric detector (4), a light source (5), and optical fibers (6). The sample test tube rack module (3) is provided with built-in test tubes (2) for holding a sample, and is also internally equipped with the reflectors (1), the reflectors (1) are located at the first sides of the test tubes (2), and the second sides of the test tubes (2) are equipped with the optical fibers (6), the optical fiber (6) have three ends, wherein one ends face the sample test tubes (2) and are located at the opposite sides of the reflectors (1), the second ends are connected to the photoelectric detector (4), and the third ends are connected to the light source (5). The reflector based fluorescent quantitative PCR detection system provided by the invention has the advantages of simple and compact structure and strong fluorescence collecting ability.
Description
Technical field
The invention belongs to biological and technical field of medical detection and in particular to a kind of compact conformation glimmering based on reflecting mirror
The detecting system of Fluorescent Quantitative PCR.
Background technology
Fluorescent quantitative PCR technique extends three so that DNA is rapidly completed degeneration, annealing and polymerization in the very short time
Process, thus realize the quick multiplication of DNA quantity.Add fluorophor in the course of reaction of PCR, then adopt different-waveband
Light irradiation sample carry out the dyestuff of excited donor and send fluorescence, the fluorescence signal collecting is converted into electricity through photodetector
Signal, and process in a computer, thus completing the quantitative analyses to DNA fluorescence, this technology is to biology and medical domain
Research have great importance.Current technology means are by dichroic plane mirror, fluorescence by the way of collecting fluorescence
Realizing, whole fluorescent collecting Optical devices need at least 3 eyeglasses to focus lens group etc..The phosphor collection device of this class formation
Complex structure, occupy bulky；If carrying out quantitative fluorescence analysis to multiple samples, optical system can be made more complicated, this
The integrated fluorescent quantitative detection device of sample will occupy more volume, cause very inconvenient in use, and collect fluorescence
The ability of signal is weaker.
Content of the invention
For solve prior art exist the problems referred to above, the invention provides a kind of structure simple and compact based on reflection
The fluorescence quantitative PCR detection system of mirror.
For reaching above-mentioned purpose, the present invention adopts the following technical scheme that：A kind of inspection of the quantitative fluorescent PCR based on reflecting mirror
Examining system, including reflecting mirror (1), sample tube frame module (3), photodetector (4), light source (5), optical fiber (6), sample tube
The built-in test tube (2) for containing sample of frame module (3), is also arranged described reflecting mirror (1) in sample tube frame module (3),
Reflecting mirror (1) is in the first side of test tube (2), the second side setting optical fiber (6) of test tube (2), and optical fiber (6) has three ends, wherein,
First end towards sample tube (2) and is in the opposite side of reflecting mirror (1), the second end connection photodetector (4), the 3rd end connection
Connect light source (5).
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, test tube (2) be provided with multiple, corresponding, instead
Penetrate mirror (1) and also be provided with polylith, both correspond laying.
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, reflecting mirror (1) is wholly transmissive to exciting light, to glimmering
Light is fully reflective.
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, the minute surface of reflecting mirror (1) is in concave shape.
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, the quantity of optical fiber (6) can be one or more
Can be corresponding with the quantity of described test tube, optical fiber is corresponded with test tube, and each optical fiber (6) is respectively installed on a structure of fiber_optic
(7) on.
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, the reflection that optical fiber (6) is positioned over reflecting mirror (1) is burnt
Point position.
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, when optical fiber (6) arranges one, optical fiber (6) is installed on
On one structure of fiber_optic (7)；Second side of test tube (2) is laid with transmission belt (9), and the two ends of transmission belt (9) are respectively cooperating with one
Root live-roller, wherein one live-roller is connected with the output shaft of motor (8)；The fixing described structure of fiber_optic of transmission belt (9)
(7), optical fiber (6) is sent to by the position corresponding with each test tube (2) by transmission belt (9), and is in corresponding reflection
The reflector focus position of mirror (1).
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, optical fiber (6) adopts y-type optical fiber.
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, light source (5) selects laser instrument, xenon lamp or LED.
The detecting system of the described quantitative fluorescent PCR based on reflecting mirror, photodetector (4) selects photodiode, light
Electric multiplier tube and ccd image sensor.
In the present invention, exciting light is exported by LED or laser instrument, and is coupled into transmission in y-type optical fiber.Excitation light irradiation
Equipped with the test tube of sample, thus the reflecting mirror after exciting sample generation fluorescence, fluorescence and exciting light to be placed on test tube is collected, swash
Light through reflecting mirror transmission, fluorescence is reflected by a reflector and focuses in y-type optical fiber；It is glimmering that y-type optical fiber is placed on reflecting mirror team
The focal point of the reflection of light, thus ensure that light as much as possible is collected by y-type optical fiber to be transferred to acquisition system.Present configuration
Compact, and improve the performance that y-type optical fiber absorbs fluorescence.
Compared with present technology, the present invention has following technology effect based on the detecting system of the quantitative fluorescent PCR of reflecting mirror
Really：
1st, present invention employs integrated reflecting mirror, each single reflecting mirror can simultaneously work as filtering and light gathers
Burnt effect, so that whole light path system is simple and compact for structure, decreases multi-disc eyeglass and light present in conventional art
The lengthy and jumbled system complex causing in road and huge problem.
2nd, the reflecting mirror that the present invention adopts has focusing and two kinds of functions of light splitting concurrently, substantially increases systematic collection fluorescence signal
Ability.
Brief description
Fig. 1 is the top view of the embodiment of the present invention 1.
Fig. 2 is the front view of the embodiment of the present invention 1.
Fig. 3 is the right view of the embodiment of the present invention 1.
Fig. 4 is the structural representation of the embodiment of the present invention 2.
Specific embodiment
In conjunction with accompanying drawing, the preferred embodiment of the present invention is elaborated.
Embodiment 1
As Figure 1-3, the present embodiment includes reflecting mirror 1, sample based on the detecting system of the quantitative fluorescent PCR of reflecting mirror
Test tube 2, sample tube frame module 3, photodetector 4, light source 5, y-type optical fiber 6, y-type optical fiber support 7, motor 8, transmission belt
9, the built-in eight vertical sample tubes 2 of sample tube frame module 3, sample tube 2 is contained within sample.Sample tube frame module 3
Inside it is also arranged eight pieces of reflecting mirrors 1, reflecting mirror 1 is curved, eight pieces of reflecting mirrors 1 are corresponded with eight sample tubes 2, vertically put
Side in sample tube 2.
The opposite side of sample tube 2 is laid with transmission belt 9, and the two ends of transmission belt 9 are respectively cooperating with a live-roller, its
In a live-roller be connected with the output shaft of motor 8, driven and rotated by motor 8, so drive transmission belt 9 be driven.
One y-type optical fiber support 7 is fixed on transmission belt 9, this structure of fiber_optic 7 is fixed a y-type optical fiber 6, y-type optical fiber 6 has
There are three terminations, wherein, one rectifies to sample tube 2, and another two ends are connected with photodetector 4, light source 5 respectively.
The exciting light that light source 5 produces transmits and irradiating sample test tube 2 (part containing sample) through y-type optical fiber 6, thus
Excited to fluorophor and produced fluorescence, exciting light and fluorescence are collected by reflecting mirror 1 simultaneously.The reflected mirror of exciting light 1 and
Transmission, fluorescence is reflected by reflecting mirror 1 and focuses in y-type optical fiber, and then fluorescence is transferred into photodetector through y-type optical fiber
4 are converted into the signal of telecommunication, the signal of telecommunication through computer process be finally obtained test sample fluorescence parameter.
Light source 5 can be from light sources such as laser instrument, xenon lamp or LED, and exciting light is to be sent and be coupled into by light source 5
To transmit in y-type optical fiber 6.
The quantity of reflecting mirror 1 is one-to-one with sample tube 2, and is placed on sample tube 2 side.Reflecting mirror 1 is right
Exciting light is wholly transmissive, fully reflective to fluorescence, and the minute surface of the reflecting mirror of reflected fluorescent light is concave surface.
In the present embodiment, the reflecting mirror in conventional solution and dichroic mirror are integrated into an entirety by reflecting mirror, and
And the quantity of reflecting mirror is one-to-one with the quantity of sample tube, and reflecting mirror is positioned over the position relative with y-type optical fiber.
The plated film type of reflecting mirror is wholly transmissive to exciting light, fully reflective to fluorescence, in order that fluorescence can focus on convenient collection,
The minute surface of reflected fluorescent light is concave surface.
Y-type optical fiber 6 is placed on the reflector focus position of reflecting mirror 1 so that fluorescent foci as much as possible is in optical fiber, real
The now maximized collection to fluorescence.
Y-type optical fiber 6 is installed on y-type optical fiber support 7, and y-type optical fiber 6 is fixing by y-type optical fiber support 7, and y-type optical fiber support 7 is solid
Dingan County is placed on transmission belt 9, and moves under motor 8 with the common driving effect of transmission belt 9, rapidly detects sample successively
The fluorescence parameter of sample in product test tube 2.
The bare headed conversion sensor such as photodiode, photomultiplier tube and ccd image sensor selected by photodetector 4.
Exciting light is derived from the light source such as laser instrument or LED, excites and is optically coupled into y-type optical fiber and transmits wherein,
Exciting light enters into sample tube from y-type optical fiber outgoing, and the fluorophor of wherein sample is excited and produces fluorescence, excites
Light and fluorescence are collected by reflecting mirror simultaneously.Because reflecting mirror plated film type is high to exciting light saturating, high to fluorescence anti-, so exciting
Light is gone out through reflecting mirror transmission, and fluorescence is reflected by a reflector.Additionally, reflecting mirror is concave surface to the reflecting surface of fluorescence, Y type light
The fine focal point just at this reflective concave surface.Fluorescent foci is coupled in y-type optical fiber, migrates in y-type optical fiber
Photodetector, photodetector can be the shaven head conversion sensing such as photodiode, photomultiplier tube and ccd image sensor
Device, by optical signal converted electrical number, the signal of telecommunication through computer process finally given test sample fluorescence parameter.When
After in a certain sample tube, sample detection finishes, y-type optical fiber and its support and detection system are in the drive of motor and transmission belt
Move to another sample tube under action, it is detected, finally complete the detection of all samples test tube one by one.
Embodiment 2
In conjunction with Fig. 4, in the present embodiment, y-type optical fiber 6 has eight groups, consistent with the quantity of sample tube, each y-type optical fiber 6
Corresponding to a sample tube 2, and each y-type optical fiber is respectively installed on a y-type optical fiber support 7.
Because, in the present embodiment, each sample tube is all correspondingly arranged a y-type optical fiber, therefore, y-type optical fiber need not move
Dynamic, you can to complete the detection of all samples test tube.Therefore, the part such as the present embodiment no motor and transmission belt.
In the present embodiment, exciting light is derived from laser instrument or LED light source, excites and is optically coupled into corresponding y-type optical fiber
And transmit wherein, exciting light enters into sample tube from corresponding y-type optical fiber outgoing, and the fluorophor of wherein sample is subject to
Produce fluorescence to exciting, exciting light and fluorescence are collected by reflecting mirror simultaneously.Because reflecting mirror plated film type is high to exciting light
Thoroughly, high to fluorescence anti-, so exciting light is gone out through reflecting mirror transmission, and fluorescence is reflected by a reflector.Additionally, reflecting mirror is to glimmering
The reflecting surface of light is concave surface, and y-type optical fiber is just at the focal point of corresponding reflective concave surface.Fluorescent foci is coupled into Y type
In optical fiber, migrate into photodetector in y-type optical fiber, photodetector can be with photodiode, photomultiplier tube and CCD
The shaven head conversion sensor such as imageing sensor, by optical signal converted electrical number, the signal of telecommunication finally gives through the process of computer
The fluorescence parameter of the sample of test.In the present embodiment, because y-type optical fiber and its support and Photodetection system are tried with sample
The quantity of pipe is one-to-one, it is therefore not necessary to mobile y-type optical fiber, you can complete the detection of all samples test tube.
The present embodiment other content refers to embodiment 1.
Fluorescence quantitative PCR detection device of the present invention has the simple and compact spy of structure with respect to existing detection means
Point, and can disposably detect all of sample.
Those of ordinary skill in the art it should be appreciated that the present invention is not restricted to the described embodiments, above-described embodiment and
The simply explanation principle of the invention described in description, without departing from the spirit and scope of the present invention, the present invention also can
There are various changes and modifications, these changes and improvements both fall within scope of the claimed invention.
Claims (10)
1. a kind of detecting system of the quantitative fluorescent PCR based on reflecting mirror, is characterized in that including reflecting mirror (1), sample tube frame
Module (3), photodetector (4), light source (5), optical fiber (6), the built-in test tube for containing sample of sample tube frame module (3)
(2), it is also arranged described reflecting mirror (1) in sample tube frame module (3), reflecting mirror (1) is in the first side of test tube (2), examination
Second side setting optical fiber (6) of pipe (2), optical fiber (6) has three ends, and wherein, first end towards sample tube (2) and is in reflection
The opposite side of mirror (1), the second end connection photodetector (4), the 3rd end connection light source (5).
2. the detecting system of the quantitative fluorescent PCR based on reflecting mirror as claimed in claim 1, is characterized in that：Described test tube (2)
It is provided with multiple, corresponding, reflecting mirror (1) also is provided with polylith, and both correspond laying.
3. the detecting system of the quantitative fluorescent PCR based on reflecting mirror as claimed in claim 1, is characterized in that：Described reflecting mirror
(1) wholly transmissive to exciting light, fully reflective to fluorescence.
4. the detecting system of the quantitative fluorescent PCR based on reflecting mirror as claimed in claim 1, is characterized in that：Described reflecting mirror (1)
Minute surface be in concave shape.
5. as described in any one of claim 2-4 the quantitative fluorescent PCR based on reflecting mirror detecting system, it is characterized in that：Described
The quantity of optical fiber (6) is corresponding with the quantity of described test tube, and optical fiber is corresponded with test tube, and each optical fiber (6) is respectively installed on one
On structure of fiber_optic (7).
6. the detecting system of the quantitative fluorescent PCR based on reflecting mirror as claimed in claim 5, is characterized in that：Described optical fiber (6)
It is positioned over the reflector focus position of reflecting mirror (1).
7. as described in any one of claim 2-4 the quantitative fluorescent PCR based on reflecting mirror detecting system, it is characterized in that：Described
Optical fiber (6) arrange one when, optical fiber (6) is installed on a structure of fiber_optic (7)；Second side of test tube (2) is laid with transmission skin
Band (9), the two ends of transmission belt (9) are respectively cooperating with a live-roller, the output shaft phase of wherein one live-roller and motor (8)
Even；Optical fiber (6) is sent to and each test tube by the fixing described structure of fiber_optic (7) of transmission belt (9) by transmission belt (9)
(2) corresponding position, and it is in the reflector focus position of corresponding reflecting mirror (1).
8. the detecting system of the quantitative fluorescent PCR based on reflecting mirror as claimed in claim 1, is characterized in that：Described optical fiber (6)
Using y-type optical fiber.
9. the detecting system of the quantitative fluorescent PCR based on reflecting mirror as claimed in claim 1, is characterized in that：Described light source (5)
From laser instrument, xenon lamp or LED.
10. the detecting system of the quantitative fluorescent PCR based on reflecting mirror as claimed in claim 1, is characterized in that：Described light electrical resistivity survey
Survey device (4) and select photodiode, photomultiplier tube and ccd image sensor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610747050.5A CN106399075B (en) | 2016-08-26 | 2016-08-26 | A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror |
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|---|---|---|---|
| CN201610747050.5A CN106399075B (en) | 2016-08-26 | 2016-08-26 | A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror |
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| CN106399075A true CN106399075A (en) | 2017-02-15 |
| CN106399075B CN106399075B (en) | 2019-05-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113960009A (en) * | 2021-09-16 | 2022-01-21 | 大连理工大学 | Capillary fluorometer with low background signal |
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| CN101464409A (en) * | 2007-12-19 | 2009-06-24 | 中国科学院电子学研究所 | Apparatus and method for fast quantitative bacteria detection |
| CN201553741U (en) * | 2009-11-16 | 2010-08-18 | 杭州博日科技有限公司 | Multiwavelength fluorescence detection device of quantitative PCR |
| US8264687B2 (en) * | 2006-02-13 | 2012-09-11 | Pacific Biosciences Of California, Inc. | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
| CN204462019U (en) * | 2015-02-06 | 2015-07-08 | 余家昌 | A kind of subminiaturization hyperchannel real-time fluorescence spectrum detection device |
| CN206109400U (en) * | 2016-08-26 | 2017-04-19 | 浙江科技学院 | Fluorescent quantitation PCR's detecting system based on speculum |
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2016
- 2016-08-26 CN CN201610747050.5A patent/CN106399075B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8264687B2 (en) * | 2006-02-13 | 2012-09-11 | Pacific Biosciences Of California, Inc. | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
| CN101464409A (en) * | 2007-12-19 | 2009-06-24 | 中国科学院电子学研究所 | Apparatus and method for fast quantitative bacteria detection |
| CN201553741U (en) * | 2009-11-16 | 2010-08-18 | 杭州博日科技有限公司 | Multiwavelength fluorescence detection device of quantitative PCR |
| CN204462019U (en) * | 2015-02-06 | 2015-07-08 | 余家昌 | A kind of subminiaturization hyperchannel real-time fluorescence spectrum detection device |
| CN206109400U (en) * | 2016-08-26 | 2017-04-19 | 浙江科技学院 | Fluorescent quantitation PCR's detecting system based on speculum |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113960009A (en) * | 2021-09-16 | 2022-01-21 | 大连理工大学 | Capillary fluorometer with low background signal |
| CN113960009B (en) * | 2021-09-16 | 2023-05-12 | 大连理工大学 | Capillary fluorometer with low background signal |
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