CN102033058B - Super resolution fluorescence lifetime imaging system - Google Patents
Super resolution fluorescence lifetime imaging system Download PDFInfo
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- CN102033058B CN102033058B CN2010105517482A CN201010551748A CN102033058B CN 102033058 B CN102033058 B CN 102033058B CN 2010105517482 A CN2010105517482 A CN 2010105517482A CN 201010551748 A CN201010551748 A CN 201010551748A CN 102033058 B CN102033058 B CN 102033058B
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Abstract
The invention is applied to the fields of optics, biology, chemistry and the like and provides a super resolution fluorescence lifetime imaging method. The method comprises the following steps of: sparsely activating an optical switch dye molecule marked in a sample; exciting the activated optical switch dye molecule in the sample; collecting photons transmitted by the activated optical switch dye molecule and recording a fluorescent image of the optical switch dye molecule; carrying out the centroid positioning on the optical switch dye molecule in the fluorescent image; counting the photons received at the centroid positioning site and determining a fluorescence lifetime of the activated optical switch dye molecule; and constructing the super resolution fluorescence lifetime image by combining the centroid positioning result with the fluorescence lifetime of the obtained optical switch dye molecule. By combining the super resolution fluorescence microtechnique based on unimolecule positioning with the fluorescence lifetime imaging based on time relevant single photon counting, the invention realizes the super resolution fluorescence lifetime imaging, breaks through the traditional optical diffraction limit and has higher scientific significance and application value.
Description
Technical field
The invention belongs to optics, biology, chemical field, relate in particular to a kind of super-resolution fluorescence life-span imaging system.
Background technology
That fluorescence microscopy has is harmless, noncontact, high specific, highly sensitive, high live body is friendly and can provide outstanding advantage such as function information, is life science always, especially the important tool of RESEARCH ON CELL-BIOLOGY.In recent years; Along with development of life science; Fluorescence microscopy has also been proposed increasingly high requirement; The continuous development of laser technology, fluorescence probe labelling technique, novel fluorescence Detection Techniques and imaging means has greatly promoted the development of fluorescence microscopy, becomes the important motivity that promotes the life science development.In addition, fluorescence microscopy is also obtaining very big progress aspect the contrast mechanism of imaging.
At present; The research of life science has got into molecular level; In order to understand the molecule mechanism of vital movement and disease progression better; Need interact between research intracellular protein position and function relationship and the protein molecule on the molecular level etc., this has higher requirement to fluorescence microscopy.The restriction of breakthrough diffraction limit, the far field fluorescence microscopy that development has the nanometer spatial discrimination has become one of the forward position focus in international micro-imaging field.The development of super-resolution (SR) imaging technique also provides huge opportunity for the new development of fluorescence lifetime imaging (FLIM).Research and development super-resolution fluorescence life-span imaging (SR-FLIM) technology; Can significantly improve the measuring accuracy and the bearing accuracy of FLIM fluorescence lifetime; Utilization is based on the FRET (FRET) of SR-FLIM; Can study the interaction between the protein molecule at molecular scale, obtain interactional efficient and accurate location thereof between the molecule.
Yet, receiving the restriction of optical diffraction limit, the spatial resolution of fluorescence microscopy can only reach about 200 nanometers, is difficult to satisfy the needs of life science.And for fluorescence lifetime imaging, its spatial resolution receives the influence of diffraction limit particularly serious.The fluorescence lifetime value of each pixel in traditional diffraction limited FLIM image can not reflect the fluorescence lifetime of its pairing molecule, can't accomplish the fluorescence lifetime imaging of molecular scale.
The imaging of super-resolution fluorescence life-span is an emerging research field, seeks to have highly original know-why and scheme, and takes the lead in solving the difficult problem that the super-resolution fluorescence life-span is imaged on existence in the life science application, has great scientific meaning and using value.
Summary of the invention
The invention provides a kind of super-resolution fluorescence life-span imaging system, be intended to break through the optical diffraction limit restriction, realize the imaging of super-resolution fluorescence life-span.
The present invention is achieved in that a kind of super-resolution fluorescence life-span imaging system, comprising:
First laser instrument is used for realizing the sparse activation to the photoswitch dye molecule that is marked on sample;
Second laser instrument is used for the photoswitch dye molecule that excited sample is activated;
Light combination mirror, light that is used for said first laser instrument is sent and said second laser instrument are sent photosyntheticly becomes a branch of light; The light that said first laser instrument is sent is certain incident angle by first reflecting surface of said light combination mirror to be injected, and reflects at first reflecting surface; The light that said second laser instrument is sent is injected by second reflecting surface of said light combination mirror, and first reflecting surface by said light combination mirror after said light combination mirror transmission penetrates;
Be located at first lens, first optical filter, catoptron between said first laser instrument and the said light combination mirror successively;
Be located at second lens, second optical filter, scanning galvanometer between said second laser instrument and the said light combination mirror successively;
First object lens, second object lens;
Be located at successively said light combination mirror first reflecting surface and said first object lens between the first pipe mirror, dichroic mirror;
The time correlation single photon counter is used for the photon that receives is counted;
Be located at the emission of first between said dichroic mirror and said time correlation single photon counter optical filter, the second pipe mirror, photomultiplier successively;
Electron multiplication type CCD camera is used for the fluoroscopic image of recording light switch dye molecule;
Be located at the emission of second between said second object lens and said electron multiplication type CCD camera optical filter, the 3rd pipe mirror;
Main frame; Connect said time correlation single photon counter and said electron multiplication type CCD camera simultaneously; Be used to combine the fluoroscopic image of the photoswitch dye molecule of said electron multiplication type CCD camera record; Photoswitch dye molecule in the fluoroscopic image is carried out barycenter location, and according to said time correlation single photon counter in the result that place, barycenter location counts the photon that receives, make up super-resolution fluorescence life diagram picture; Also connect said first laser instrument and said second laser instrument simultaneously, be used to control the work of said first laser instrument and said second laser instrument;
And
Delayer is connected between said main frame and the said electron multiplication type CCD camera, is used to realize the synchronous of said second laser instrument and said electron multiplication type CCD camera.
The present invention will combine based on the super-resolution fluorescence microtechnic of unimolecule location with based on the fluorescence lifetime imaging of time correlation single photon counting, realize the imaging of super-resolution fluorescence life-span, break through the optical diffraction limit restriction, have scientific meaning and using value.
Description of drawings
Fig. 1 is the realization flow figure of the super-resolution fluorescence life-span formation method that provides of the embodiment of the invention;
Fig. 2 is the optical structure chart of the super-resolution fluorescence life-span imaging system that provides of the embodiment of the invention.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with accompanying drawing and embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
The basic ideas of the embodiment of the invention are with combining based on the micro-STORM technology of the super-resolution fluorescence of unimolecule location with based on the fluorescence lifetime imaging of time correlation single photon counting (TCSPC), realizing super-resolution fluorescence life-span imaging (SR-FLIM).
Describe in detail below in conjunction with specific embodiment of the present invention.
Fig. 1 shows the realization flow of the super-resolution fluorescence life-span formation method that the embodiment of the invention provides, and details are as follows:
In step S101, the photoswitch dye molecule that is marked in the sample is carried out sparse activation.
Wherein, in photoswitch dye molecule and the sample the molecule that will observe need and can specificity combine, can select red cyanine dye molecule for use, like Cy5 and Alexa 647, the mode through immunofluorescence dyeing with red cyanine dye molecular labeling in sample.Also can select for use the structure picture that utilizes the cis-trans isomeride to change the fluorescin cause invertible switch, like Dronpa series, the mode through transfection with fluorescent protein labeling in sample.
In the embodiment of the invention, specifically can be through the method exciting light switch dye molecule of whole audience face illumination.
In step S102, the photoswitch dye molecule that is activated in the excited sample, the photon that the photoswitch dye molecule that collection is excited is launched and the fluoroscopic image of recording light switch dye molecule carry out the barycenter location to the photoswitch dye molecule in the fluoroscopic image.
Can adopt the picopulse photoscanning to excite the fluorescence molecule that is activated; Because the embodiment of the invention is with combining based on the micro-STORM technology of the super-resolution fluorescence of unimolecule location with based on the fluorescence lifetime imaging of time correlation single photon counting (TCSPC); Therefore the photon of the photoswitch dye molecule that is excited being launched need be collected two parts: portion is used for the fluoroscopic image of recording light switch dye molecule; Single light emitting molecule in the sample is carried out the barycenter location, realize laterally reaching nano level bearing accuracy; Another part then be used to realize to individual molecule the accurate classification of luminous son, through adopting the negative exponential function match, thereby can obtain the fluorescence lifetime accurately of each light emitting molecule, fitting formula is I (t)=I
0Exp (t/ τ), wherein, t is the time, τ is a fluorescence lifetime, I
0Be the fluorescence intensity of initial time (during t=0), I (t) is a t fluorescence intensity constantly.
The purpose of barycenter location is to realize laterally reaching nano level bearing accuracy.Though for microscopic system; The picture of a pointolite is the Airy disk by the decision of system point spread function; But the locus of pointolite can be obtained through the barycenter of its fluoroscopic image; The square root from the photon number of this point source that bearing accuracy (standard deviation) and system detect is inversely proportional to, and is directly proportional with the standard deviation of system point spread function own, therefore can obtain the bearing accuracy up to nanometer.And horizontal barycenter location just directly just can be realized with the Gaussian function match; Axially unimolecule is located some axial supplementary means of differentiating of micro-combination; For example transform point spread function, make disalignment carry the coordinate information of z axle, for example utilize the cylindrical mirror astigmatism to locational point spread function (PSF); Spiral PSF also can bring up to 50 nanometers even higher level with axial resolution.
In step S103, the photon that receives at barycenter location place is counted the fluorescence lifetime of the photoswitch dye molecule of confirming to be excited.
Described in the principle such as preceding text step S102 of count results calculating fluorescence lifetime, repeat no more here.
In step S104,, make up super-resolution fluorescence life diagram picture in conjunction with the barycenter positioning result and the fluorescence lifetime of the photoswitch dye molecule that obtains.
Life-span and locating information are combined to reconstruct the fluorescence lifetime image of super-resolution, and for example the point of each in the image has been represented the position of each molecule, and the color of this institute's respective pixel can be represented the fluorescence lifetime of this molecule.
Fig. 2 shows the optical structure chart of the super-resolution fluorescence life-span imaging system that the embodiment of the invention provides, and for the ease of describing, only shows the part relevant with present embodiment.English tag definitions among Fig. 2 is following: Laser1: first laser instrument; Laser2: second laser instrument (emission picopulse exciting light); L1: first lens; L2: second lens; F1: first optical filter; F2: second optical filter; Scan Device: scanning galvanometer; M: catoptron; DM: dichroic mirror; BS: light combination mirror; TL1: the first pipe mirror; TL2: the second pipe mirror; TL3: the 3rd pipe mirror; O1: first object lens; O2: second object lens; S: sample; TCSPC: time correlation single photon counter; Delay: delayer; PMT: photomultiplier; EMCCD: electron multiplication type CCD camera; EF1: the first emission optical filter; EF2: the second emission optical filter.
The above-mentioned first laser instrument Laser1 is used for realizing the sparse activation to the photoswitch dye molecule that is marked on sample, and the second laser instrument Laser2 then is used for the photoswitch dye molecule that excited sample is activated.The light that light combination mirror BS is used for the first laser instrument Laser1 is sent and the second laser instrument Laser2 are sent photosyntheticly becomes a branch of light; Wherein the light that sent of the first laser instrument Laser1 is certain incident angle by first reflecting surface (right flank of light combination mirror BS among Fig. 2) of light combination mirror BS and injects; And reflect at first reflecting surface; The light that the second laser instrument Laser2 is sent is injected by second reflecting surface (left surface of light combination mirror BS among Fig. 2) of light combination mirror BS, and first reflecting surface by light combination mirror BS after light combination mirror BS transmission penetrates.The first lens L1, the first optical filter F1, mirror M are located between the first laser instrument Laser1 and the light combination mirror BS successively, and the second lens L2, the second optical filter F2, scanning galvanometer Scan Device are located between the second laser instrument Laser2 and the light combination mirror BS successively.The first pipe mirror TL1, dichroic mirror DM are located between first reflecting surface and the first object lens O1 of light combination mirror BS successively.Time correlation single photon counter TCSPC is used for the photon that receives is counted.The first emission optical filter EF1, the second pipe mirror TL2, photomultiplier PMT are located between dichroic mirror DM and the time correlation single photon counter TCSPC successively.Electron multiplication type CCD camera EMCCD is used for the fluoroscopic image of recording light switch dye molecule.The second emission optical filter EF2, the 3rd pipe mirror TL3 are located between the second object lens O2 and the electron multiplication type CCD camera EMCCD.Main frame is relevant single photon counter TCSPC of tie-time and electron multiplication type CCD camera EMCCD simultaneously; Be used to combine the fluoroscopic image of the photoswitch dye molecule of electron multiplication type CCD camera EMCCD record; Photoswitch dye molecule in the fluoroscopic image is carried out the barycenter location; And, make up super-resolution fluorescence life diagram picture according to the result that time correlation single photon counter TCSPC counts the photon that receives at barycenter location place; Main frame also connects the first laser instrument Laser1 and the second laser instrument Laser2 simultaneously, controls the work of the first laser instrument Laser1 and the second laser instrument Laser2.Delayer Delay is connected between main frame and the electron multiplication type CCD camera EMCCD, is used to realize the synchronous of said second laser instrument and said electron multiplication type CCD camera.
With reference to Fig. 2, it is the CW semiconductor laser of 640 nanometers that Laser1 can select output wavelength for use, as the activating light source of switch molecule.In light path, the method for employing face illumination utilizes object lens O1 that exciting light is converged on the sample S.Through power and the activationary time etc. of control exciting light, the sparse activation of realization switch molecule.Laser2 is that output wavelength is the picopulse semiconductor laser of 640 nanometers, as the excitation source of the switch molecule that is activated.In light path, the picopulse exciting light focuses on the sample S, does quick scanning through galvanometer Scan Device along sample, is used to excite the switch molecule that is activated.The light that sample S is sent is collected with relative two object lens O1 and O2, sends into PMT and EMCCD respectively.The former sends the photon signal that receives into TCSPC, is used for the counting of photon; The latter obtains the fluoroscopic image of sparse activating molecules and image is transferred to main frame, and main frame can obtain the accurate position of light emitting molecule through the barycenter location algorithm.Activate the switching of light path and excitation light path through host computer control; And TCSPC detect with the EMCCD imaging synchronously, specifically each cyclic process is: Laser1 sends laser so that the photoswitch dye molecule is carried out sparse activation, and--the photoswitch dye molecule that is activated in the Laser2 excited sample--EMCCD imaging---TCSPC counts.
Experimental procedure is following:
(1) utilizes powerful CW laser Laser1; Sample S is carried out the illumination of whole audience face; Controller through Laser1 or the neutral density filter (not shown among Fig. 2) of being located at said first optical filter front side or rear side are controlled illumination intensity; Control lighting hours through shutter, realize sparse activation photoswitch dye molecule in the sample.
(2) utilize low power picosecond pulse laser Laser2, and sample is scanned the switch molecule that has been activated in the excited sample through scanning galvanometer Scan Device.
(3) since sample at whole spatial emission photon, the basic thinking that realizes this project super-resolution imaging is to carry out molecule location earlier, then with photon " distribution " to correct molecule.Therefore, to the fluorescence that activating molecules sent, collect by O1 and two object lens of O2 respectively.The photon that the O2 object lens are collected adopts common STORM formation method, and unimolecule is positioned analysis, obtains the super-resolution fluorescence micro-image; The photon of O1 object lens is received by photomultiplier PMT, sends into time correlation single photon counter TCSPC and carries out fluorescence lifetime measurement.
(4) repeating step 1-3 all is positioned and durability analysis finishes until all molecules.Obtain the super-resolution fluorescence life diagram picture of sample through image reconstruction.
The present invention will combine based on the super-resolution fluorescence microtechnic of unimolecule location with based on the fluorescence lifetime imaging of time correlation single photon counting; Realize the imaging of super-resolution fluorescence life-span; Broken through the optical diffraction limit restriction; Have scientific meaning and using value, with effectively promoting the development of China at life science.
The above is merely preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. a super-resolution fluorescence life-span imaging system is characterized in that, comprising:
First laser instrument is used for realizing the sparse activation to the photoswitch dye molecule that is marked on sample;
Second laser instrument is used for the photoswitch dye molecule that excited sample is activated;
Light combination mirror, light that is used for said first laser instrument is sent and said second laser instrument are sent photosyntheticly becomes a branch of light; The light that said first laser instrument is sent is certain incident angle by first reflecting surface of said light combination mirror to be injected, and reflects at first reflecting surface; The light that said second laser instrument is sent is injected by second reflecting surface of said light combination mirror, and first reflecting surface by said light combination mirror after said light combination mirror transmission penetrates;
Be located at first lens, first optical filter, catoptron between said first laser instrument and the said light combination mirror successively;
Be located at second lens, second optical filter, scanning galvanometer between said second laser instrument and the said light combination mirror successively;
First object lens, second object lens;
Be located at successively said light combination mirror first reflecting surface and said first object lens between the first pipe mirror, dichroic mirror;
The time correlation single photon counter is used for the photon that receives is counted;
Be located at the emission of first between said dichroic mirror and said time correlation single photon counter optical filter, the second pipe mirror, photomultiplier successively;
Electron multiplication type CCD camera is used for the fluoroscopic image of recording light switch dye molecule;
Be located at the emission of second between said second object lens and said electron multiplication type CCD camera optical filter, the 3rd pipe mirror;
Main frame; Connect said time correlation single photon counter and said electron multiplication type CCD camera simultaneously; Be used to combine the fluoroscopic image of the photoswitch dye molecule of said electron multiplication type CCD camera record; Photoswitch dye molecule in the fluoroscopic image is carried out barycenter location, and according to said time correlation single photon counter in the result that place, barycenter location counts the photon that receives, make up super-resolution fluorescence life diagram picture; Also connect said first laser instrument and said second laser instrument simultaneously, be used to control the work of said first laser instrument and said second laser instrument;
And
Delayer is connected between said main frame and the said electron multiplication type CCD camera, is used to realize the synchronous of said second laser instrument and said electron multiplication type CCD camera.
2. imaging system as claimed in claim 1 is characterized in that, also comprises the neutral density filter of being located at said first optical filter front side or rear side.
3. imaging system as claimed in claim 1 is characterized in that, said first laser instrument is the continuous light laser instrument.
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|---|---|---|---|---|
| CN102692401B (en) * | 2012-06-06 | 2014-09-03 | 中国科学院半导体研究所 | Gating fluorescence service life imaging device based on light delay |
| CN102998290B (en) * | 2012-11-14 | 2015-08-05 | 深圳大学 | A kind of fluorescence lifetime micro imaging system |
| CN102937743A (en) * | 2012-11-26 | 2013-02-20 | 上海交通大学 | Random optics reconstruction fluorescent microscope and implementation method thereof |
| CN103163106B (en) * | 2013-01-30 | 2014-12-17 | 浙江大学 | Super-resolution fluorescent lifetime imaging method and device based on stimulated emission lost |
| CN103344617B (en) * | 2013-06-17 | 2015-05-06 | 重庆大学 | Photoactivated single-molecular fluorescence microscope for biochemical reaction kinetics and test method |
| CN103592278B (en) * | 2013-11-21 | 2016-01-20 | 中国计量学院 | Based on random position super-resolution microscopic method and the device of fluorescent emission inhibiting mechanism |
| CN103901012A (en) * | 2014-04-23 | 2014-07-02 | 山西大学 | Method and device for improving definition in nano particle fluorescence imaging |
| CN105823765A (en) * | 2016-03-21 | 2016-08-03 | 天津大学 | Centroid Algorithm for Scalable Fluorescence Lifetime Detection |
| CN106053405B (en) * | 2016-05-10 | 2018-10-02 | 东南大学 | A kind of super-resolution optical imaging method based on unimolecule positioning mode |
| CN106772428B (en) * | 2016-12-15 | 2019-04-16 | 哈尔滨工业大学 | A kind of non-ken three-dimensional image forming apparatus of no-raster formula photon counting and method |
| CN107013872A (en) * | 2017-03-07 | 2017-08-04 | 上海斐讯数据通信技术有限公司 | A kind of energy-conserving road lamp, energy-saving street lamp control system and control method |
| CN106885795B (en) * | 2017-03-08 | 2020-03-27 | 深圳大学 | Method and system for acquiring fluorescence lifetime information of moving single particles |
| CN107478630B (en) * | 2017-09-12 | 2020-04-17 | 山西大学 | Device and method for improving single-molecule optical imaging contrast |
| FR3073050B1 (en) | 2017-11-02 | 2023-06-30 | Centre Nat Rech Scient | APPARATUS AND METHOD FOR SUPER-RESOLUTION FLUORESCENCE MICROSCOPY AND FLUORESCENCE LIFETIME MEASUREMENT |
| CN108120702B (en) * | 2017-11-30 | 2020-08-11 | 浙江大学 | Super-resolution fluorescence lifetime imaging method and device based on parallel detection |
| CN108333157B (en) * | 2018-01-23 | 2021-08-03 | 深圳大学 | Method and system for three-dimensional dynamic analysis of biomolecules |
| CN111521596B (en) * | 2020-06-04 | 2021-02-05 | 深圳大学 | Fluorescence differential super-resolution imaging method and imaging system |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101435774A (en) * | 2008-12-23 | 2009-05-20 | 中国科学院上海光学精密机械研究所 | Method and device of dual-color single photon transverse super resolution imaging |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0023619D0 (en) * | 2000-09-27 | 2000-11-08 | Amersham Pharm Biotech Uk Ltd | Measurement of fluorescence decay times |
| GB2404013B (en) * | 2003-07-17 | 2006-05-31 | Isis Innovation | Apparatus for and method of measuring fluorescence lifetime |
-
2010
- 2010-11-19 CN CN2010105517482A patent/CN102033058B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101435774A (en) * | 2008-12-23 | 2009-05-20 | 中国科学院上海光学精密机械研究所 | Method and device of dual-color single photon transverse super resolution imaging |
Non-Patent Citations (2)
| Title |
|---|
| 林子扬等.一种实现显微荧光寿命图的测量方法.《光学技术》.2005,第31卷(第3期),326-329. * |
| 毛峥乐等.超分辨远场生物荧光成像——突破光学衍射极限.《中国激光》.2008,第35卷(第9期),1283-1306. * |
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