CN107576639A - Portable fully integrated DNA spot examines micro-full analytical system light path - Google Patents
Portable fully integrated DNA spot examines micro-full analytical system light path Download PDFInfo
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Abstract
The present invention relates to a kind of portable fully integrated DNA spot to examine micro-full analytical system light path, it is characterised in that the light path includes exciting light focusing system, micro-fluidic electrophoresis chip, phosphor collection and focuses on conducting system, fluorescence spectrophotometer and detection system.The fluorescence of wide wavelength can be divided into multiple color bands by the present invention；Fluorescent light beam can be focused to micron-sized hot spot by the focus lamp in wavelength division multiplexer simultaneously, suitable for low noise detector and because sensitive area is big and detector that noise is high.The present invention has the light channel structure that high flux, high flexibility, the process debugging of multiple fluorescence channels are simple, cost is low, module independent, process debugging is simple, stability is good, and electrophoresis path and the expansible degree of fluorescence channel are high, can be widely applied to the specific application areas such as DNA detections, Site Detection, capillary electrophoresis detection.
Description
Technical field
The present invention relates to a kind of portable fully integrated DNA spot to examine micro-full analytical system light path, belongs to biology
Detection technique field.
Background technology
DNA sequencing technology is the Biophysical techniques used in the engineerings such as nucleic acid, albumen, cell.In DNA sequencing skill
In art, capillary electrophoresis separation technology makes the DNA segment of different length move in the duct at different rates, fluoroscopic examination skill
Art is to marking the different DNA segment of the length of different fluorescence informations to carry out the multi parameter analysis of electrophoresis pattern.Microchip technology is
Based on analytical chemistry, using MEMS process technology as rely on, by micro-full analytical chip system technology with it is integrated
Technology combines, and the functions such as sampling, dilution, sample introduction, separation, detection are integrated on microchip, realizes portability, intelligent
Operation.DNA sequencing technology, which has, is included in multinomial application in molecular biology, phytobiology field, and with scene,
Miniaturization, integrated microchip DNA testing laboratories are to realizing that the Site Detection in forensic dna field provides possibility.
2011, national DNA examination of material evidences amount was 1783339 parts, and national public security organ shares DNA reviewers 1505
Name, for inspected number, the workload and pressure of reviewer is all very big, and many major and serious criminal cases generally require to work overtime all night
Examine, to provide the clue and evidence of science for case in time.DNA spot is examined micro-full analytical system to incorporate DNA and carried
The processes such as-PCR amplifications-Capillary Electrophoresis are taken, it can carry out the inspection of the samples such as buccal swab and blood cake, realize " sample enters-
As a result go out " Automated inspection, whole flow process completed in 2 hours, can carry out 4~6 parts of sample surveys simultaneously.Whole DNA sequences
The micro flow control chip capillary electrophoresis detection platform of row includes following critical piece：Microcurrent controlled capillary tube electrophoresis chip, electrophoresis lead to
Road 2-10 bars, DNA segment is carried by running gel and flows through electrophoresis path；Fluorescence detection optical path system, it is coupled to electrophoresis chip
Scanning area, the DNA segment that detection passes through electrophoresis pipeline；Conversion system, be converted into by the signal for exporting light path based on
The accessible data of calculation machine；Computer, for analyzing the data generated by converting system.Fluorescence detection optical path is mainly by exciting
Light path and phosphor collection and sensing light path composition, excitation light path provide focus on light beam, usually the laser of single wavelength or
The laser of multiple wavelength, therefore can have one or more light source；It is glimmering that phosphor collection and sensing optical routing collect object lens collection
Light light beam is simultaneously detected by one or more detector.
Cofocus scanning mode is a kind of fluorescence detection optical path structure of prevalence in the world, can carry out multichannel, large area
(100 millimeters × 100 millimeters), intermediate resolution (5 microns~50 microns), detection speed it is relatively fast (<Miniflow 100kHz)
Body chip signal detects, and stores generation digital image, but its traditional critical component scanning collection object lens is complicated, price
Costliness, such as document Citation:The confocal scanning gleanings used in Rev.Sci.Instrum.81,113105 (2010)
Mirror, micro objective is formed by 5 a diameter of 5mm lens, it is expensive complicated up to 100,000 RMB, processing technology；Select
The same costliness of Nikon, Plan Fluor camera lenses price is up to 60,000 RMB, and volume is big, and quality is big, requires high to motor, for a long time
Scanning stabilization is poor.Conventional laser is expensive, and volume is big and power consumption.Such as Publication No. CN205368375U patents use
Fiber coupling output type laser, by laser emitting laser coupled into optical fiber, and collimated by the collimating mirror of fiber end face,
The method can reduce the light utilization efficiency of laser, reduce system sensitivity, while laser because of optical coupling efficiency and optical transmission loss
Coupling testing and measuring technology to optical fiber is complicated.And it is nearest, laser diode " LD " can use.It is different from conventional laser,
LD of new generation has cost benefit high, compact-sized and save, and adapts to the demand of the portable Biomedical Instruments of a new generation.LD launches
The hot spot of oval cross section, wherein, commonly referred to as the transverse of fast axle and is commonly referred to as slow perpendicular to LD joint
Joint of the ellipse short shaft of axle parallel to LD.And for current chip Capillary Electrophoresis, by the multiple reversion and scanning of light path
After object lens focus on, oval-shaped beam can be reliably focused into, and micro-fluidic electrophoresis chip passage longitudinal scanning point can be improved
Resolution.The light path of existing capillary electrophoresis detection platform mostly uses vibration mirror scanning mode, and fluorescence enters inspection after grating beam splitting
Device CCD or CMOS are surveyed, such as patent CN104641217A discloses a kind of flow cytometer, it is disclosed that big numerical aperture
Footpath, the combination of the fluorescence microscope and WDM of wide output collimated light beam carry out fluoroscopic examination to the cell that marked fluorescence, and WDM is used
Split in multi-wavelength fluorescence, but the relay imaging mirror concave mirror of its WDM embedding structure, the purpose is to make collimated light beam
Path effectively doubles, and without expanded light beam diameter, but this is bound at incident fluorescence with cemented doublet to eliminate
Aberration caused by concave mirror, it is complicated system optics to be present in this design structure, and debugging is difficult, will to machining accuracy
Ask high, machine cost is high, and optics cost is also high.
In summary, for the microcurrent controlled capillary tube chip in fully integrated DNA spot checking system, in the presence of certain office
It is sex-limited, the integrated of micro-fluidic chip and detecting system can not be realized, does not reach portable fully integrated DNA micro-analysis systems detection
It is required that subject matter is：1) apply in micro flow control chip capillary electrophoresis, light source uses spot light, will in radial resolving power
In the case of seeking height, laser power requires high, and cost is high；2) vibration mirror scanning mode, it is desirable to which operating distance is short, objective focal length
It is small, therefore swash width can be made short, electrophoresis path flux is small, and efficiency reduces, and testing and measuring technology difficulty is big, if it is desired to efficiency is improved,
Then requiring the focal length increase of scanning objective, then numerical aperture reduces, and fluorescence sensitivity reduces, instrument fluorescent collecting hydraulic performance decline,
Sample size increase is then required, extraction, amplification, the efficiency of the several steps of electrophoresis will improve, so that whole process difficulty increases；3) adopt
With poly-lens objective, object lens volume is big, and quality is big, and cost is high, and complete machine stability reduces；4) grating beam splitting is used, it is desirable to enter
The quasi- value property of the fluorescence of grating is good, high to the performance requirement of object lens, and complex designing, technology difficulty is big, and cost is high, while uses grating
Light splitting, stop position is unique with respect to object lens, and space requirement is big, and space arrangement is dumb；Matching detector, to improve
Detection sensitivity, CCD or CMOS carry refrigeration module, and volume is big, and cost is high；5) space light splitting pattern, take up space big, and position
Put unique.For above prior art shortcoming and existing limitation, the integrated of micro-fluidic chip and detecting system can not be realized,
The requirement of portable fully integrated DNA micro-analysis systems detection is not reached.
The content of the invention
In view of the above-mentioned problems, it is an object of the invention to provide the expansible degree of a kind of electrophoresis path and fluorescence channel is big, passage
Between the good portable fully integrated DNA spot of detection signal uniformity examine micro-full analytical system light path.
To achieve the above object, the present invention takes following technical scheme：A kind of portable fully integrated DNA spot is examined miniature
Analysis system light path, it is characterised in that the light path includes：Exciting light focusing system, the exciting light focus on system
System includes laser, laser fluorescence dichroscope, total reflection prism and aspheric surface object lens, and the laser of the laser output is through standard
The laser fluorescence dichroscope is transmitted into after straight, through being all-trans described in the laser signal warp of laser fluorescence dichroscope outgoing
Penetrate prismatic reflection and enter the aspheric surface object lens, laser is converted into ellipse light spot and focused on micro-fluidic by the aspheric surface object lens
The electrophoresis path of electrophoresis chip, the detection sample being arranged in the electrophoresis path, which is excited, sends fluorescence, wherein, it is described to be all-trans
Penetrate mirror and be fixedly mounted on a mechanical structured member with aspheric surface object lens, the mechanical structured member is driven in a plane by motor
Do the scanning of one-dimensional square；Phosphor collection focuses on conducting system, and the phosphor collection, which focuses on conducting system, also includes fluorescent foci
Mirror and multimode fibre, the aspheric surface object lens collimate to the fluorescence signal of collection, and the fluorescence signal after collimation is through described complete
The laser fluorescence dichroscope is transmitted into after reflecting prism total reflection, the laser fluorescence dichroscope passes through the fluorescence excited
The fluorescent foci mirror, which focuses on, enters the multimode fibre；Fluorescence spectrophotometer and detection system, the fluorescence spectrophotometer and detection system
Including wavelength division multiplexer and detector, the wavelength division multiplexer is used to collimate the fluorescence from multimode fibre outgoing, and by width
The fluorescence of wave-length coverage is divided into multiple color bands and exported by different passages, and the detector is used to detect through the wavelength-division multiplex
The fluorescence of the different passages of device output.
Further, the wavelength division multiplexer includes the first~the N of optical fiber interface, laser filter and cross arrangement
Passage spectral module, each passage spectral module include dichroscope, filter and focus lamp；The multimode fibre will
Broadband fluorescence through the laser filter is dealt into first passage dichroscope after being collimated, through the first passage two to
The fluorescence of Look mirror reflection focuses on through first passage filter and first passage focus lamp successively, through the first passage dichroscope
The fluorescent emission of transmission is to second channel dichroscope；The fluorescence reflected through the second channel dichroscope is filtered through second channel
Look mirror and second channel focus lamp focus on, the fluorescent emission through second channel dichroscope transmission to third channel dichroic
Mirror ... the like, broadband fluorescence exports N number of color band through N number of passage and detected by the corresponding detector.
Further, the multimode fibre is connected by ceramic insertion core with the optical fiber interface of the wavelength division multiplexer.
Further, the multimode fibre is supported regulation by optical fiber three-dimensional adjustable shelf.
Further, it is using the optical element that can be collimated to light beam, the optics to optical signal collimation
Collimation lens, the collimation lens use planoconvex spotlight, spherical mirror, aspherical mirror or double glued mirrors.
Further, the laser uses solid state laser, optical fiber laser or diode laser.
Further, the single focal length F of the aspheric surface object lens is 2~6mm, numerical aperture 0.60, the aspherical thing
Mirror uses planoconvex lens, biconvex mirror or balsaming lens.
Further, the fluorescent foci mirror uses cemented doublet, and the focal length f of the cemented doublet is about 30mm.
Further, the detector uses single channel or multi channel detector.
Further, the total reflection prism uses 45 degree of right-angle prisms, through laser fluorescence dichroscope outgoing
A wherein right-angle surface for light beam through 45 degree of right-angle prisms occurs total reflection on inclined-plane and changes beam Propagation direction so that X
The laser that direction is propagated prolongs Z-direction after 45 degree of right-angle prisms reflection and propagated, and total reflection light beam is sent out through another right-angle surface
It is mapped to the aspheric surface object lens.
For the present invention due to taking above technical scheme, it has advantages below：1st, exciting light focusing system of the invention will
The micron order light that diode laser focuses on by collimating mirror, laser fluorescence dichroscope, total reflection prism and aspheric surface object lens
Spot focuses on the electrophoresis path center of micro-fluidic electrophoresis chip, and the fluorescence that electrophoresis path center inspires is focused on by phosphor collection and passed
Guiding systems are collected and are conducted to fluorescence spectrophotometer and detection system after focusing on multimode fibre wire jumper, and the present invention passes through total reflection prism
Addition make axial plane that 90 degree upsets occur, solve scan position and excitation source and fluorescent foci conducts position cannot
The problem of coplanar, the selection of laser fluorescence dichroscope can make excitation light path and fluorescence light path be split up into difference in same plane
Direction.2nd, laser of the invention is using small volume, the laser diode of output ellipse light spot, and collimated lens are to fast axle side
To after collimation, the ellipse light spot of 3 μm of 5 μ m is finally focused to by aspherical mirror, with respect to electrophoresis path, the present invention, which improves, radially to be divided
While resolution, the power of laser can be reduced to 60mW, while improving radial direction electrophoresis DNA sequence resolution, reduce and swash
Luminous power, so as to reduce Overall Power Consumption and cost.3rd, the aspheric surface object lens of object lens large-numerical aperture of the invention, it is with being all-trans
Matched diodes laser, micro-fluidic electrophoresis chip and linear scanning motor after prism arrangement are penetrated, reaches the spirit of detecting system fluorescence
Sensitivity, electrophoresis path flux and DNA sequence dna resolution requirement, scanning signal stability improves and detecting system is steady in a long-term
Property and reliability strengthen.4th, the present invention uses the rectilinear scan pattern of linear electric motors, is matched with small size aspheric surface object lens so that
The expansible degree of scanning field of view scope increases, in addition, the operating distance of aspheric surface object lens of the present invention is small, so as to reduce bulk,
To adapt to the size of micro-fluidic electrophoresis chip and structure design；Further, linear electric motors coordinate the scan mode of object lens also to reduce
To the design requirement of object lens, optical quality and raising of the Laser Focusing hot spot at micro-fluid control chip electrophoretic pipeline are improved
The quality of fluorescence hot spot after fluorescent foci mirror, the symmetry, stability and uniformity of output signal are improved, be easy to rear end to turn
The signal transacting of change system.5th, phosphor collection of the invention focuses on carry out phosphor collection of the conducting system based on multimode fibre, body
Product is small, and locus is flexible, can shorten the propagation light path of fluorescence, reaches the phosphor collection efficiency of complicated microcobjective camera lens.
6th, fluorescence spectrophotometer of the present invention and detection system use wavelength division multiplexer, its internal structure according to the port number of system fluorescence channel,
The wavelength and bandwidth characteristic of fluorescence channel carry out light splitting design, make it more portable, can with modularization, miniaturization, in limited sky
Between in the range of expansible degree is big, fluorescence efficiency is higher, replaceability is strong so that complete machine structure and volume, the feelings of size constancy
The fluorescence of wide wavelength can be divided into multiple color bands by flexible expansion or reduction fluorescence channel number, the present invention under condition；Ripple simultaneously
Fluorescent light beam can be focused to micron-sized hot spot by the focus lamp in division multiplexer, suitable for low noise detector and because of sensitivity
Face is big and detector that noise is high.To sum up, the present invention has high flux, high flexibility, the process debugging letter of multiple fluorescence channels
Light channel structure single, cost is low, module independent, process debugging is simple, and stability is good, can be widely applied to DNA and detects, be existing
The specific application areas such as field detection, capillary electrophoresis detection.
Brief description of the drawings
The portable fully integrated DNA spot that Fig. 1 is the present invention examines micro-full analytical system light path schematic diagram；
Fig. 2 is wavelength division multiplexer (WDM) structural representation of the present invention；
Fig. 3 is the focal beam spot schematic diagram of the laser of the present invention；
Fig. 4 is the focal beam spot schematic diagram of the fluorescent foci mirror of the present invention；
Fig. 5 is the DNA sequence dna parting collection of illustrative plates design sketch of the present invention.
Embodiment
Come to carry out the present invention detailed description below in conjunction with accompanying drawing.It should be appreciated, however, that accompanying drawing has been provided only more
Understand the present invention well, they should not be interpreted as limitation of the present invention.Wherein, X-direction of the invention refer to parallel to
The X-axis in rectangular coordinate system in the plane of horizontal plane；Y-direction refers to the rectangular coordinate system in the plane parallel to horizontal plane
In Y-axis；Z-direction refers to perpendicular to the axial direction of horizontal plane.
Portable fully integrated DNA spot provided by the invention examines micro-full analytical system light path, including exciting light
Focusing system, micro-fluidic electrophoresis chip, phosphor collection focus on conducting system, fluorescence spectrophotometer and detection system.
As shown in figure 1, exciting light focusing system includes laser 1, collimating mirror 2, laser fluorescence dichroscope 3, total reflection
Prism 4 and aspheric surface object lens 5.The collimated mirror 2 of laser that laser 1 exports is transmitted into laser fluorescence dichroscope 3 after collimating, and passes through
The laser signal that laser fluorescence dichroscope 3 is emitted is reflected into aspheric surface object lens 5 through total reflection prism 4, and aspheric surface object lens 5 will
Laser is converted into ellipse light spot and focuses on the electrophoresis path of micro-fluidic electrophoresis chip 6, the detection sample being arranged in electrophoresis path
Product are excited fluorescence.Wherein, completely reflecting mirror 4 of the invention is fixedly mounted on a mechanical structured member with aspheric surface object lens 5, machine
Tool structural member is driven the scanning that one-dimensional square can be done in a plane by linear scanning motor so that scanning focused
Optical quality is good, hot spot optical density distribution in different scanning position is consistent, so that signal conformance between different electrophoresis paths
Good, the optimal swash width scope maximum of fluorescence signal of the present invention can reach 20mm, 8 electrophoresis of Scanning Detction can lead to simultaneously
Road.
Phosphor collection, which focuses on conducting system, also includes fluorescent foci mirror 7 and multimode fibre 8.Aspheric surface object lens 5 are to collection
Fluorescence signal is collimated, and fluorescence signal is transmitted into laser fluorescence dichroscope 3, laser fluorescence after the total reflection of completely reflecting mirror 4
Dichroscope 3 focuses on the fluorescence excited into multimode fibre 8 through fluorescent foci mirror 7, because light beam is entirely being propagated in light path
Diffusion, multimode fibre 8 is because of its small volume, and locus is flexible, can shorten the propagation light path of fluorescence, so as to make up and can reach
The phosphor collection efficiency of complicated microcobjective camera lens.
Fluorescence spectrophotometer and detection system include wavelength division multiplexer (WDM) 9 and detector 10.Wavelength division multiplexer (WDM) 9 uses
The conventional wavelength division multiplex device in optical communication field, for collimating the fluorescence being emitted from multimode fibre 8, and by the glimmering of wide wave-length coverage
Light is divided into multiple color bands and exported by different passages, and detector 10 is used to detect through the output of wavelength division multiplexer (WDM) 9 not
With the fluorescence signal of passage.
In a preferred embodiment, as shown in Fig. 2 wavelength division multiplexer (WDM) 9 includes optical fiber interface 91, collimating mirror
92nd, laser filter 93, the first~the N channel spectral module, five fluorescence spectrophotometer modules are used in the embodiment of the present invention, with this
Exemplified by, not limited to this.Each passage spectral module includes dichroscope, filter and focus lamp；Multimode fibre 8 is by broadband
Fluorescence be sent to collimating mirror 92 and collimated, the fluorescence after collimation is dealt into first passage dichroscope 941 through laser filter,
The fluorescence reflected through first passage dichroscope 941 focuses on through first passage filter 942 and first passage focus lamp 943 successively
As small light spot, through the fluorescent emission that first passage dichroscope 941 transmits to second channel dichroscope 951；It is logical through second
The fluorescence that road dichroscope 951 reflects focuses on through second channel filter 952 and second channel focus lamp 953 turns into small light spot,
The fluorescent emission transmitted through second channel dichroscope 952 to third channel dichroscope 961, the like, it is broadband glimmering
Light exports five color bands through five passages and detected by corresponding detector, and passage spectral module of the invention intersects row
Cloth, to reduce space, detector 10 can be subsequently used to separately detect the fluorescence signal of five fluorescence channels.
In a preferred embodiment, multimode fibre 8 can pass through ceramic insertion core 11 and wavelength division multiplexer (WDM) 9
Optical fiber interface 91 connects, and multimode fibre 8 can be supported regulation by optical fiber three-dimensional adjustable shelf.
In a preferred embodiment, collimation lens 2 can use planoconvex spotlight, spherical mirror, aspherical mirror, double gluings
The optical element that mirror etc. can be collimated to light beam.
In a preferred embodiment, laser 1 uses small volume, exports the diode laser of ellipse light spot, this
The spot size of diode laser used is invented as 2.5mm × 1.2mm, the angle of divergence is 0.4mrad × 0.6mrad, hot spot
Major axis is 1.2mm, a length of 0.7mm of short axle, after collimated lens 2 collimate, is finally focused to 3 μm of 5 μ m by aspheric surface object lens 5
Ellipse light spot, as shown in figure 3, the present invention can also use other lasers such as solid state laser, optical fiber laser.
In a preferred embodiment, the single focal length F of aspheric surface object lens 5 is about 2~6mm, numerical aperture 0.60, is gathered
Burnt energy density is high, and small volume is in light weight, and scanning reliability is high, to adapt to the design feature of micro-fluidic electrophoresis chip 6, aspheric
Face object lens 5 can also use planoconvex lens, biconvex mirror, balsaming lens or the object lens of other poly-lenses combination.
In a preferred embodiment, fluorescent foci mirror 7 uses cemented doublet, and the focal length f of cemented doublet is about
30mm, it is about 10 times with respect to the multiplication factor of aspheric surface object lens 5, and imaging point range figure is as shown in figure 4, will according to system sensitivity
Summation resetting difficulty can use multimode fibre 7 of the fibre core for 50~200um.
In a preferred embodiment, detector 10 can use single channel or multi channel detector, such as PMT, light
Sub- register, photocell or photodiode.
In a preferred embodiment, total reflection prism 4 can use 45 degree of right-angle prisms, through laser fluorescence dichroic
A wherein right-angle surface for the light beam that mirror 3 is emitted through 45 degree of right-angle prisms 4 is totally reflected on inclined-plane, and light beam changes transmission side
The laser propagated to so that X-direction prolongs Z-direction propagation after prismatic reflection, and the light beam of total reflection is through another right-angle surface along non-
Sphere object lens 5 focus on micro-fluidic electrophoresis chip 6.
Through experiment, portable fully integrated DNA spot inspection micro-full analytical system of the invention can detect 8 electrophoresis and lead to
Road, 5 fluorescence channels, total is dexterous, and simply, cost is low, and testing and measuring technology is simple, stability is high.The whole inspection of the present invention
The fluorescence sensitivity of examining system can reach 1nM, and the resolution ratio of DNA sequence dna reaches 1bp, experimental result as shown in Figure 5.
The various embodiments described above are merely to illustrate the present invention, wherein the structure of each part, connected mode and manufacture craft etc. are all
It can be varied from, every equivalents carried out on the basis of technical solution of the present invention and improvement, should not exclude
Outside protection scope of the present invention.
Claims (10)
1. a kind of portable fully integrated DNA spot examines micro-full analytical system light path, it is characterised in that the light path
Including：
Exciting light focusing system, the exciting light focusing system include laser, laser fluorescence dichroscope, total reflection prism and
Aspheric surface object lens, the laser of the laser output is collimated to be transmitted into the laser fluorescence dichroscope, through the laser
The laser signal of fluorescence dichroscope outgoing is reflected into the aspheric surface object lens, the aspherical thing through the total reflection prism
Laser is converted into ellipse light spot and focuses on the electrophoresis path of micro-fluidic electrophoresis chip by mirror, is arranged in the electrophoresis path
Detection sample, which is excited, sends fluorescence, wherein, the completely reflecting mirror is fixedly mounted on a mechanical structured member, institute with aspheric surface object lens
State the scanning that mechanical structured member does one-dimensional square by motor driving in a plane；
Phosphor collection focuses on conducting system, and the phosphor collection, which focuses on conducting system, also includes fluorescent foci mirror and multimode fibre,
The aspheric surface object lens collimate to the fluorescence signal of collection, and the fluorescence signal after collimation is after completely reflecting mirror total reflection
The laser fluorescence dichroscope is transmitted into, the laser fluorescence dichroscope gathers the fluorescence excited through the fluorescent foci mirror
Jiao enters the multimode fibre；
Fluorescence spectrophotometer and detection system, the fluorescence spectrophotometer and detection system include wavelength division multiplexer and detector, the wavelength-division
Multiplexer is used to collimate the fluorescence from multimode fibre outgoing, and the fluorescence of wide wave-length coverage is divided into multiple color bands and passed through
Different passage outputs, the detector are used for the fluorescence for detecting the different passages through wavelength division multiplexer output.
2. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature exists
In, the wavelength division multiplexer includes the first~the N channel spectral module of optical fiber interface, laser filter and cross arrangement,
Each passage spectral module includes dichroscope, filter and focus lamp；The multimode fibre is by broadband fluorescence
First passage dichroscope is dealt into through the laser filter after being collimated, through the glimmering of first passage dichroscope reflection
Light is focused on through first passage filter and first passage focus lamp successively, and the fluorescence through first passage dichroscope transmission is sent out
It is mapped to second channel dichroscope；Fluorescence through second channel dichroscope reflection is logical through second channel filter and second
Road focus lamp focuses on, the fluorescent emission through second channel dichroscope transmission to third channel dichroscope ... successively class
Push away, broadband fluorescence exports N number of color band through N number of passage and detected by the corresponding detector.
3. portable fully integrated DNA spot as claimed in claim 2 examines micro-full analytical system light path, its feature exists
In the multimode fibre is connected by ceramic insertion core with the optical fiber interface of the wavelength division multiplexer.
4. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature exists
In the multimode fibre is supported regulation by optical fiber three-dimensional adjustable shelf.
5. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature exists
In to optical signal collimation using the optical element that can be collimated to light beam, the optics is collimation lens, the standard
Straight lens use planoconvex spotlight, spherical mirror, aspherical mirror or double glued mirrors.
6. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature exists
In the laser uses solid state laser, optical fiber laser or diode laser.
7. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature exists
It is 2~6mm in the single focal length F of, aspheric surface object lens, numerical aperture 0.60, the aspheric surface object lens are using planoconvex lens, double
Convex lens or balsaming lens.
8. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature exists
In the fluorescent foci mirror uses cemented doublet, and the focal length f of the cemented doublet is about 30mm.
9. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature exists
In the detector uses single channel or multi channel detector.
10. portable fully integrated DNA spot as claimed in claim 1 examines micro-full analytical system light path, its feature
It is, the total reflection prism uses 45 degree of right-angle prisms, and the light beam through laser fluorescence dichroscope outgoing is described in
A wherein right-angle surface for 45 degree of right-angle prisms occurs total reflection on inclined-plane and changes beam Propagation direction so that what X-direction was propagated swashs
Light prolongs Z-direction after 45 degree of right-angle prisms reflection and propagated, and total reflection light beam is transmitted into the aspheric through another right-angle surface
Face object lens.
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| CN108579829A (en) * | 2018-04-25 | 2018-09-28 | 合肥工业大学 | Exempt from pump type micro-fluidic chip and preparation method thereof and portable biochemical analyser |
| CN108844936A (en) * | 2018-07-09 | 2018-11-20 | 领航基因科技（杭州）有限公司 | A kind of three-dimensional drop detection system and detection method |
| CN110006860A (en) * | 2019-03-27 | 2019-07-12 | 华南师范大学 | A kind of burnt multichannel fluorescence detecting system of copolymerization |
| CN114047170A (en) * | 2021-11-27 | 2022-02-15 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114486691A (en) * | 2022-02-16 | 2022-05-13 | 上海纬冉科技有限公司 | Portable energy detection equipment and debugging device thereof |
| CN115747035A (en) * | 2023-01-05 | 2023-03-07 | 苏州和迈精密仪器有限公司 | Continuous scanning monitoring analysis system based on discrete three-dimensional fluorescence technology |
| WO2023077306A1 (en) * | 2021-11-03 | 2023-05-11 | 深圳华大智造科技股份有限公司 | Detection device, gene sequencing system, and detection method |
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| CN114047170B (en) * | 2021-11-27 | 2022-08-16 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
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