CN203053867U - Fluorescence spectrum-time-resolved fluorescence simultaneous detection fiber optic spectrometer - Google Patents
Fluorescence spectrum-time-resolved fluorescence simultaneous detection fiber optic spectrometer Download PDFInfo
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- CN203053867U CN203053867U CN 201320026852 CN201320026852U CN203053867U CN 203053867 U CN203053867 U CN 203053867U CN 201320026852 CN201320026852 CN 201320026852 CN 201320026852 U CN201320026852 U CN 201320026852U CN 203053867 U CN203053867 U CN 203053867U
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Abstract
The utility model discloses a fluorescence spectrum-time-resolved fluorescence simultaneous detection fiber optic spectrometer. The fiber optic spectrometer comprises a light path part, a circuit part and an industrial personal computer (IPC), wherein the light path part comprises a light source controller, a light source, a sample disk, a monochromator and a light detector, which are connected in sequence; the circuit part comprises a light source control circuit, a spectral data acquisition and analysis system and a time-resolved circuit, which are connected with the IPC; the light source controller is connected with the light source control circuit; and the light detector is connected with the spectral data acquisition and analysis system and the time-resolved circuit respectively. The fiber optic spectrometer has the advantages of small volume, convenience in carrying, flexibility of application sites and low cost and can conveniently detect and monitor the samples in real time.
Description
Technical field
The utility model relates to a kind of fluorescence spectrum-time-resolved fluorescence detection fiber spectrometer simultaneously, belongs to the optical analysis technical field.
Background technology
Fluorescence refers to some compound after the optical excitation that is subjected to certain wavelength, launches wavelength in the short time greater than the light of excitation wavelength at the utmost point.Utilize this phenomenon, can realize highly sensitive detection to this compounds, detection technique of fluorescence has increasing application in medical treatment in residues of pesticides and the Environmental Studies.Fluoroscopic examination is a kind of non-contacting indirect light spectral technology, and highly sensitive, specificity is good.In recent years, some researchers have also developed the Pesticides Testing instrument based on detection technique of fluorescence, and for example, usefulness optical fiber immunofluorescence assays such as Richard have been measured dichlorophenoxyacetic acid residual in water, and detection sensitivity reaches 0.68 * 10
-4μ g/L; Renee etc. have developed field portable formula three-dimensional fluorescence spectrum instrument, have obtained palycyclic aromatic PAH
sThe three-dimensional fluorescence spectrogram, the measurement wavelength coverage is 220nm～950nm, lowest detectable limit has reached 1.6 * 10
-4μ g/L; Pepper etc. utilize induced with laser and taper printing opacity technology to develop a kind of multichannel optical fiber fluorescence detecting system, and use it for on-site measurement soil surface pollutant, he has designed special-purpose fibre-optical probe namely can surface measurement, also can carry out the soil internal measurement, and utilize grating beam splitting and CCD light spectrum image-forming, can once obtain two dimension or three-dimensional fluorescence spectrum, spectral range is the 300-450nm spectral range.Laser Induced Fluorescence Technology will provide higher sensitivity for detection owing to adopted this intense light source of laser as exciting light.
Fluorescent emission is a kind of common radiation deactivation process, typically refers to electronics and is subjected to after the optical excitation from high-energy state to emit the process of photon simultaneously to the low-energy state transition, and the time of this process is usually at l0
-10～l0
-7S.Utilize optical instrument to detect fluorescent emission intensity over time, can obtain the fluorescence lifetime information of system.The life-span of fluorophor depends on the character of fluorophor.When containing multiple fluorescent material in the system, because situation overlapping and that disturb may appear in the fluorescence emission spectrum of each material, rely on common fluorescence emission spectrum means possibly can't obtain system information accurately separately.Utilize the fluorescence TIME RESOLVED TECHNIQUE, can parse the composition situation of fluorescent material in the system by the difference of fluorescence lifetime, thereby valuable information is provided.Even if fluorophore of the same race, because its microenvironment difference of living in, its fluorescence lifetime also may have very big difference.Therefore, utilize the fluorescence TIME RESOLVED TECHNIQUE, can distinguish the fluorophore of the same race that is in different microenvironments.Scientist has been applied to fluorescence time resolved spectroscopy technology the measurement of mixed system fluorescence lifetime and the structure elucidation of parsing and protein in recent years.Gray etc. utilize dansyl Cl labeled cell pigment C, and utilize the FRET of itself and heme, measure the variation of its fluorescence lifetime, thereby have provided several typical configurations of protein, and proposed the structural change process in the protein folding procedure.
Also there not be at present both at home and abroad to report the analytical instrument that fluorescence spectrum and time-resolved fluorescence technology based on optical fiber technology combine.
The utility model content
The purpose of this utility model provides a kind of fluorescence spectrum-time-resolved fluorescence detection fiber spectrometer simultaneously, this spectrometer adopts flexibly, and fibre-optical probe carries out original position, plane, stereoscanning with realization to sample, and take fluorescence frequency domain spectrum and time-resolved fluorescence spectral technology to combine this technology path to reduce the fluorescence interference of background background, realize specific detection and the on-line continuous of sample are detected.
A kind of fluorescence spectrum provided by the utility model-time-resolved fluorescence is the detection fiber spectrometer simultaneously, comprises light path part, circuit part and industrial computer;
Described light path part comprises light source controller, light source, sample disc, monochromator and the photodetector that connects successively;
Described circuit part comprises control circuit for light source, spectrum data gathering analytic system and time resolution circuit, and described control circuit for light source, described spectrum data gathering analytic system and described time resolution circuit all are connected with described industrial computer;
Described light source controller is connected with described control circuit for light source; Described photodetector is connected with described time resolution circuit with the spectrum data gathering analytic system respectively.
In the above-mentioned fiber spectrometer, can be connected by optical fiber between described light source and the described sample disc.
In the above-mentioned fiber spectrometer, described monochromator is connected with the spectral scan control circuit, and described spectral scan control circuit is connected with described industrial computer.
In the above-mentioned fiber spectrometer, described photodetector specifically can be photomultiplier;
Described monochromator is connected with stepper motor, by the rotation of grating in the described monochromator of described step motor control, sets necessary wavelength, arranges and carries out fluorescence decay curve, calculates the life-span of fluorescence.
In the above-mentioned fiber spectrometer, described photodetector specifically can be diode array detector.
In the above-mentioned fiber spectrometer, described control circuit for light source, described spectrum data gathering analytic system and described time resolution circuit all are connected with described industrial computer by communication controller.
Compared with prior art, the beneficial effects of the utility model are:
1) the utility model can be realized the analysis of fluorescence on frequency domain and time domain, replenish mutually, improve the accuracy of fluorescence analysis on qualitative and quantitative greatly, and set up the examination database of known natural fluoresence material fluorescence spectrum and the storehouse complementation of time-resolved fluorescence spectrum;
2) the utility model is combined optical fiber technology with the time-resolved fluorescence technology, and is applied to morphological analysis, has realized rig-site utilization and monitoring in real time;
3) the utility model adopts optical fiber scanning, has overcome because of sample to move the problem that toilet not brings.
It is little to the utlity model has volume, easy to carry, and site of deployment is flexible, and the advantage that cost is low can detect in real time and monitor sample easily.
Description of drawings
Fig. 1 is fluorescence spectrum of the present utility model-time-resolved fluorescence structural representation of detection fiber spectrometer simultaneously.
Fig. 2 is the index path of photomultiplier for photodetector in the utility model.
Fig. 3 is the index path of diode permutation detecting device for photodetector in the utility model.
1 light-pulse generator controller, 2 light sources, 3 sample disc, 4 monochromators, 5 photodetectors, 6 control circuit for light source, 7 spectral scan control circuits, 8 spectrum data gathering analytic systems, 9 time resolution circuit, 10 communication controllers, 11 industrial computers, 12 optical fiber, 13 slits, 14 gratings, 15 stepper motors, 16 reflective mirrors, 17 photomultipliers, 18, diode array detector.
Embodiment
Below in conjunction with accompanying drawing the utility model is described further, but the utility model is not limited to following examples.
As shown in Figure 1, the utility model comprises light path part, circuit part and industrial computer 11; Wherein, light path part comprises pulse power source control device 1, light source 2, sample disc 3, monochromator 4 and the photodetector 5 that connects successively, circuit part comprises control circuit for light source 6, spectral scan control circuit 7, spectrum data gathering analytic system 8 and the time resolution circuit 9 that all is connected with industrial computer 11, and control circuit for light source 6, spectral scan control circuit 7, spectrum data gathering analytic system 8 and time resolution circuit 9 all are connected with industrial computer 11 by communication controller 10.Pulse power source control device 1 is connected with control circuit for light source 6, and the output pulse of this control circuit for light source 6 gating pulse power-supply controller of electric and continuous light signal are to light source 2; Light source 2 and sample disc 3 are connected by optical fiber; When exciting light by fiber-optic illuminated sample in sample disc 3 on after, by the grating in the monochromator 4 or prism, enter again in the photodetector 5, wherein, monochromator 4 is connected with spectral scan control circuit 7.Photodetector 5 is connected with time resolution circuit 9 with spectrum data gathering analytic system 8 respectively.
When photodetector was chosen as photomultiplier in the utility model, optic path as shown in Figure 2.The common optical fiber 12 of crossing of the exciting light of the light source 1 that can modulate, shine sample after, shine on the grating 14 of monochromator after excited fluorescent another port by optical fiber arrives slit 13, through exciting light with launch light and separate.Realize spectral scan if desired, can be by the angle of stepper motor 15 control gratings 4, the emission light after the monochrome can shine photomultiplier 17 by catoptron 16 and realize that light detects.
When photodetector was chosen as diode array in the utility model, optic path as shown in Figure 3.The common optical fiber 12 of crossing of the exciting light of the light source 1 that can modulate, after shining sample, shine on the grating 14 of monochromator behind excited fluorescent another port arrival slit 13 by optical fiber, separate with emission light through exciting light, the spectral illumination after the separation obtains spectral signal on diode array detector 18.
Utilize the utility model to realize that the method for fluorescence spectrum detection is as follows:
By control circuit for light source, light source is set to normal on-mode, and under this pattern, light source is not modulated, is normally open.By the rotation of step motor control grating, carry out spectral scan.
Utilize the utility model to realize that the method for time resolution fluorescence spectral detection is as follows:
By control circuit for light source, light source is modulated, be set to the flicker pattern, the frequency of flicker is 1000Hz.By the rotation of step motor control grating, set required wavelength, arrange and carry out fluorescence decay curve, calculate the life-span of fluorescence, and determine specific.By the record fluorescence decay curve, calculate fluorescence lifetime; In the time-resolved fluorescence detection system, by comparer, the time interval length records between two electric signal can be got off, and transform into electric signal, and note.Duplicate record several 10
3-10
4, draw fluorescence decay curve.
Claims (6)
1. a fluorescence spectrum-time-resolved fluorescence detection fiber spectrometer simultaneously, it is characterized in that: described fiber spectrometer comprises light path part, circuit part and industrial computer;
Described light path part comprises light source controller, light source, sample disc, monochromator and the photodetector that connects successively;
Described circuit part comprises control circuit for light source, spectrum data gathering analytic system and time resolution circuit, and described control circuit for light source, described spectrum data gathering analytic system and described time resolution circuit all are connected with described industrial computer;
Described light source controller is connected with described control circuit for light source; Described photodetector is connected with described time resolution circuit with the spectrum data gathering analytic system respectively.
2. fiber spectrometer according to claim 1 is characterized in that: be connected by optical fiber between described light source and the described sample disc.
3. fiber spectrometer according to claim 1 and 2, it is characterized in that: described monochromator is connected with the spectral scan control circuit, and described spectral scan control circuit is connected with described industrial computer.
4. fiber spectrometer according to claim 3, it is characterized in that: described photodetector is photomultiplier;
Described monochromator is connected with stepper motor.
5. fiber spectrometer according to claim 3, it is characterized in that: described photodetector is diode array detector.
6. fiber spectrometer according to claim 1 and 2, it is characterized in that: described control circuit for light source, described spectrum data gathering analytic system and described time resolution circuit all are connected with described industrial computer by communication controller.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320026852 CN203053867U (en) | 2013-01-18 | 2013-01-18 | Fluorescence spectrum-time-resolved fluorescence simultaneous detection fiber optic spectrometer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320026852 CN203053867U (en) | 2013-01-18 | 2013-01-18 | Fluorescence spectrum-time-resolved fluorescence simultaneous detection fiber optic spectrometer |
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| CN203053867U true CN203053867U (en) | 2013-07-10 |
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| CN 201320026852 Expired - Fee Related CN203053867U (en) | 2013-01-18 | 2013-01-18 | Fluorescence spectrum-time-resolved fluorescence simultaneous detection fiber optic spectrometer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105588826A (en) * | 2016-02-24 | 2016-05-18 | 中国科学院物理研究所 | Femtosecond time resolution multi-channel lock-phase fluorescence spectrophotometer based on optical parametric amplification |
| CN107121394A (en) * | 2017-07-11 | 2017-09-01 | 江西农业大学 | The fluorescence spectrum detecting device of poultry veterinary drug residue |
-
2013
- 2013-01-18 CN CN 201320026852 patent/CN203053867U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105588826A (en) * | 2016-02-24 | 2016-05-18 | 中国科学院物理研究所 | Femtosecond time resolution multi-channel lock-phase fluorescence spectrophotometer based on optical parametric amplification |
| CN105588826B (en) * | 2016-02-24 | 2019-03-26 | 中国科学院物理研究所 | A kind of femtosecond time resolution multiple tracks locking phase Fluorescence Spectrometer based on optically erasing |
| CN107121394A (en) * | 2017-07-11 | 2017-09-01 | 江西农业大学 | The fluorescence spectrum detecting device of poultry veterinary drug residue |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20220118 |