CN104181133A - Multi-wavelength capillary electrophoresis fluorescence excitation device - Google Patents
Multi-wavelength capillary electrophoresis fluorescence excitation device Download PDFInfo
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- CN104181133A CN104181133A CN201310201409.5A CN201310201409A CN104181133A CN 104181133 A CN104181133 A CN 104181133A CN 201310201409 A CN201310201409 A CN 201310201409A CN 104181133 A CN104181133 A CN 104181133A
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Abstract
The invention discloses a multi-wavelength capillary electrophoresis fluorescence excitation device. The device is composed of multiple groups of excitation light sources, a multi-channel optical fiber coupler and an output optical fiber. Each group of the excitation light source is composed of a high-brightness light-emitting diode, a focusing unit and a coupling optical fiber. The input ends of the multi-channel optical fiber coupler are connected with the coupling optical fibers of the excitation light sources, and light rays of multiple channels are converged and coupled and then are output by the output optical fiber. The output end of the output optical fiber extends into a detection window position directly from a detection end port of a capillary of an electrophoresis apparatus, so as to realize fluorescence excitation through illuminating from the detection window interior. Wave bands of the high brightness light-emitting diodes adopted by the excitation light sources are not consistent, and excitation lights with wavelengths of a visible or near ultraviolet region can be emitted through providing power to the different light-emitting diodes, so as to adapt to maximum excitation wavelengths of different fluorescence probes. The device realizes wave band switching through an electronic means, and has the characteristics of simple structure, low cost and high stability.
Description
Technical field
The invention belongs to biological chemistry Micrometry, be specifically related to a kind of multi-wavelength Capillary Electrophoresis fluorescence excitation device.
Background technology
High performance capillary electrophoresis (High-performance capillary electrophoresis, HPCE) be one of the fastest analytical approach of development in recent years, there is separation efficiency high, fast and the amount of samples of velocity of separation such as lacks at the advantage, is widely used in the fields such as peptide and protein, environment food, DNA sequence analysis and unicellular monitoring.Because active volume and the sample size of HPCE are few, detecting device is had to very high requirement, apply at present the more method that has uv absorption, galvanochemistry, mass spectrum and fluoroscopic examination.Fluoroscopic examination is as the technology of a kind of high sensitivity, high selectivity, and in trace and microscratch sample detection, application is very extensive.
Fluorescent capillary electrophoresis tube instrument mainly consists of capillary electrophoresis, fluorescence detector, fluorescent exciting source apparatus.At present, excitation source adopts gas-discharge lamp, gas energizer light source, semiconductor solid state laser etc. conventionally, but has the feature that size is large, energy consumption is high, cost is expensive.In recent years, high brightness LED (light-emitting diode, LED) has caused people's concern gradually as a kind of miniature of economy.LED has the advantages such as stable output power, energy consumption is low, volume is little, brightness is high, wavelength coverage is wider, the life-span is long, is suitable as very much fluorescence excitation light source.LED as excitation source performance close to common coherent source, each wave band of high-brightness LED nearly cover, can, by selecting suitable LED to adapt to the maximum excitation wavelength of fluorescence probe, realize the fluoroscopic examination to biological samples such as protein, DNA and amino acid.LED irradiates capillary detection window and fluorescence excitation from kapillary outside after mostly adopting focusing as the optical texture of fluorescence excitation light source, unavoidably in capillary surface generation anaclasis and scattering process, make the light being sent by LED can not be fully for fluorescence excitation, and then cause higher background noise, affect the sensitivity of fluorescence excitation efficiency and capillary electrophoresis apparatus.
The exciting light that LED is sent is introduced into and is positioned at capillary detection window and realizes fluorescence excitation by light transmitting fiber, have advantages of highly sensitive, background noise is low, choosing wavelength wider range, structure and simple to operate, in the capillary electrophoresis apparatus having, used at present this technology, present way is for different detection material, utilize mechanical hook-up (being generally round turntable) to switch different types of LED before the condenser lens of optical fiber, select different excitation wavelengths.This mode structure is complicated a little, is unfavorable for miniaturization and the microminiaturization of electrophoresis apparatus, and the anti-seismic performance of instrument and life-span limited by physical construction, easily damage.
Summary of the invention
The object of the present invention is to provide a kind of multi-wavelength Capillary Electrophoresis fluorescence excitation device.It has feature simple in structure, with low cost, stability is high.
A kind of multi-wavelength Capillary Electrophoresis fluorescence excitation device provided by the invention (7) forms by organizing excitation source (1), a multi-channel optical fibre coupling mechanism (2) and an output optical fibre (3) more.Every group of LASER Light Source (1) is comprised of a high brightness LED (4), a focalizer (5) and a coupled fiber (6).The high brightness light emitting diode (4) of every group of excitation source (1) has different emergent light wave bands, the light that high brightness LED (4) sends is injected focalizer (5), focalizer (5) imports light into coupled fiber (6), an input end of the other end access multi-channel optical fibre coupling mechanism (2) of coupled fiber (6); The output terminal of many group excitation sources (1) connects respectively the input end of multi-channel optical fibre coupling mechanism (2), then from output optical fibre (3), exports.
The output terminal of output optical fibre (3) directly stretches into detection window (15) position from the detection port of electrophoresis apparatus kapillary (9), realizes and carries out fluorescence excitation from detection window (15) internal radiation.
By giving respectively different high brightness LED (4) power supplies, the present invention can launch the exciting light of visible range or each wave band of near ultraviolet region, the maximum excitation wavelength that adapts to different fluorescence probes, realizes the fluoroscopic examination to biological samples such as protein, DNA and amino acid.Utilize electronically to replace mechanical hook-up and realize wave band switching, there is feature simple in structure, with low cost, stability is high.
Accompanying drawing explanation
Fig. 1 is the structural drawing of multi-wavelength Capillary Electrophoresis fluorescence excitation device.
Fig. 2 is the use schematic diagram of multi-wavelength Capillary Electrophoresis fluorescence excitation device.
Embodiment
Below in conjunction with accompanying drawing, say the concrete embodiment of multi-wavelength Capillary Electrophoresis fluorescence excitation device.
The mechanism of multi-wavelength Capillary Electrophoresis fluorescence excitation device (7) as shown in Figure 1.In Fig. 1,1 is excitation source, and 2 is multi-channel optical fibre coupling mechanism, and 3 is output optical fibre.Excitation source (1) consists of a high brightness LED (4), a focalizer (5) and a coupled fiber (6), the light that high brightness LED (4) sends is injected focalizer (5), focalizer (5) imports light into coupled fiber (6), an input end of the other end access multi-channel optical fibre coupling mechanism (2) of coupled fiber (6); The output terminal of many group excitation sources (1) connects respectively the input end of multi-channel optical fibre coupling mechanism (2), then from output optical fibre (3), exports.
The effect of multi-channel optical fibre coupling mechanism (2) is that multichannel input is optically coupled in together, outwards exports.The high brightness light emitting diode (4) of every group of excitation source (1) has different emergent light wave bands.When the high brightness LED (4) in many groups excitation source (1) only has one to light, the wave band of output light is consistent with the wave band of this high brightness LED (4), by electronic switching electricity mode, control the light on and off of high brightness LED (4), just can reach the object that changes output optical fibre (3) medium wavelength.
Fig. 2 is the use schematic diagram of multi-wavelength Capillary Electrophoresis fluorescence excitation device, represents how multi-wavelength Capillary Electrophoresis fluorescence excitation device (7) is used in Capillary Electrophoresis fluorescence detector.As shown in Figure 2, Capillary Electrophoresis fluorescence detector consists of multi-wavelength Capillary Electrophoresis fluorescence excitation device (7), Capillary Electrophoresis part, fluorescence detection device (16) and data processing equipment (17).
Capillary Electrophoresis part: formed by Buffer Pool (8), kapillary (9), detection cell (10), DC high-voltage power supply (11) and platinum electrode (12,13).It is upper that the test section of kapillary (9) is fixed on protective substrate (14), and have detection window (15).The effect of DC high-voltage power supply (11) is to provide electric field, drives test substance swimming, realizes the efficient separation of material.After micro substance sample introduction to be measured, uniflux under the effect of electric field force, because the charge characteristic of different materials is different, thereby suffered electric field force is different, the speed of swimming is also just different, causes the asynchronism(-nization) of material arrival detection window (15), thereby the separation on implementation space.The output terminal of the output optical fibre of multi-wavelength Capillary Electrophoresis fluorescence excitation device directly stretches into detection window position from electrophoresis apparatus detection port capillaceous, exciting light is incorporated into kapillary (9) inside, the test substance that irradiating and detecting window (15) is located, realizes fluorescence excitation.Fluorescence is collected by fluorescence detection device (16), is transformed into electric signal, then sends data processing equipment (17) to and processes and analyze.
Fluorescence detection device (16) is generally comprised of focusing objective len, optical filter and photomultiplier.After being responsible for fluorescence signal to collect, be transformed into electric signal, while processing for later analysis.Carrying out opto-electronic conversion is mainly completed by photomultiplier.
Data processing equipment (17) is generally a computing machine, is responsible for gathering and analysis of fluorescence signal, realizes qualitative and quantitative analysis.
Claims (2)
1. a multi-wavelength Capillary Electrophoresis fluorescence excitation device, forms by organizing excitation source (1), a multi-channel optical fibre coupling mechanism (2) and an output optical fibre (3) more; Every group of LASER Light Source is comprised of a high brightness LED (4), a focalizer (5) and a coupled fiber (6); It is characterized in that: the light that described high brightness LED (4) sends is injected focalizer (5), focalizer (5) imports light into coupled fiber (6), an input end of the other end access multi-channel optical fibre coupling mechanism (2) of coupled fiber (6); The output terminal of many group excitation sources (1) connects respectively the input end of multi-channel optical fibre coupling mechanism (2), then from output optical fibre (3), exports.
2. LASER Light Source as claimed in claim 1 (1), the high brightness light emitting diode (4) of every group of excitation source (1) has different emergent light wave bands.
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| CN201310201409.5A CN104181133A (en) | 2013-05-24 | 2013-05-24 | Multi-wavelength capillary electrophoresis fluorescence excitation device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105973859A (en) * | 2016-06-29 | 2016-09-28 | 山东科立森生物股份有限公司 | Capillary electrophoresis detection system and method |
| CN105973860A (en) * | 2016-06-29 | 2016-09-28 | 山东科立森生物股份有限公司 | Capillary electrophoresis detection system and method |
| CN114450586A (en) * | 2019-08-07 | 2022-05-06 | 生命技术公司 | Multi-capillary optical detection system for capillary electrophoresis |
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| CN105973859A (en) * | 2016-06-29 | 2016-09-28 | 山东科立森生物股份有限公司 | Capillary electrophoresis detection system and method |
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| CN114450586A (en) * | 2019-08-07 | 2022-05-06 | 生命技术公司 | Multi-capillary optical detection system for capillary electrophoresis |
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