WO2018000664A1

WO2018000664A1 - Capillary electrophoresis detection system and detection method

Info

Publication number: WO2018000664A1
Application number: PCT/CN2016/102707
Authority: WO
Inventors: 金见睿; 万戈江; 冯栋
Original assignee: 山东科立森生物股份有限公司
Priority date: 2016-06-29
Filing date: 2016-10-20
Publication date: 2018-01-04
Also published as: CN105973860A; CN105973860B

Abstract

A capillary electrophoresis detection system and detection method, the system comprising at least two lasers (101); one capillary (102), a detection sample in the capillary (102) comprising a plurality of target substances to be detected, each of which comprising a biological substance labeled with a fluorescent dye or a biological substance binding an antibody labeled with a fluorescent dye; and a plurality of detectors (103). A laser ray outputted from the at least two lasers (101) irradiates on the capillary (102), and the fluorescent dye in the test sample in the capillary (102) is excited by the laser ray to generate fluorescence in a plurality of wavelength ranges, and the plurality of detectors (103) receive the fluorescence in a plurality of wavelength ranges so as to detect the type and content of the plurality of target substances to be detected as contained in the detection sample in the capillary. At least two lasers are used in the method to irradiate the capillary. As the wavelength of the laser ray outputted by each laser is different, the number of wavelength ranges that can excite fluorescence herein is greater than the number of wavelength ranges that can excite fluorescence in the case of just one laser, thereby increasing the detection speed, and saving detection samples.

Description

Capillary electrophoresis detection system and detection method

The present application claims priority to Chinese Patent Application No. 201610496480.4, entitled "Capillary Electrophoresis Detection System and Detection Method", filed on June 29, 2016, the entire contents of which are incorporated herein by reference. In the application.

Technical field

The invention relates to the field of detection technology, in particular to a capillary electrophoresis detection system and a detection method.

Background technique

Capillary electrophoresis (CE) technology is a new liquid phase separation technology with a capillary as a separation channel and a high-voltage DC electric field as the driving force. Capillary electrophoresis generally uses a quartz capillary column. Under the action of a high voltage electric field outside the capillary, the buffer on one side moves toward the negative electrode due to positive charge. At the same time, in the buffer solution, the charged biomolecules move in the direction of the electrode opposite to the charge polarity of the charged biomolecule at different speeds under the action of a high voltage electric field outside the capillary to form an electrophoresis.

In the prior art, a capillary electrophoresis technique is combined with a fluorescent labeling analysis technique to detect a substance contained in a sample. A fluorescent dye is labeled on a biological substance. During the movement of the biological substance in the capillary, the fluorescent dye is excited by the light source to generate fluorescence, the detector receives the generated fluorescence, and the received fluorescence is analyzed to detect the type of the target substance to be tested in the sample in the capillary. Or content. At present, the commonly used detection method is to use a laser to excite the fluorescence generated by a fluorescent dye on a sample in a capillary tube for detection, and only one target substance to be tested can be detected at a time. If there are a large number of target substances to be tested in the test sample that need to be detected, the test can only be divided into multiple times, the detection speed is slow, and the test sample is expensive.

Summary of the invention

The technical problem to be solved by the present invention is to provide a capillary electrophoresis detection system and a detection method, which can detect a large amount of biological substances in the same detection sample at one time, and the detection speed is fast, and the detection sample consumption is small.

To this end, the technical solution of the present invention to solve the technical problem is:

A capillary electrophoresis detection system, the system comprising:

At least two lasers each having a different wavelength of the output; a capillary tube, the detection sample in the capillary tube comprising a plurality of target substances to be tested, the target substance to be tested comprising a biological substance labeled with a fluorescent dye or a labeled substance a biological substance of an antibody to a fluorescent dye; a plurality of detectors;

The laser light output by the at least two lasers is irradiated on the capillary, and the fluorescent dye in the detection sample in the capillary is excited by the laser to generate fluorescence of a plurality of wavelength ranges, and the plurality of detectors receive a plurality of wavelength ranges Fluorescence, which in turn detects the type or content of a plurality of target substances to be tested included in the test sample in the capillary.

Optionally, each detector includes:

a filter, and a photodetector;

Each filter transmits fluorescence in a range of wavelengths, and each photodetector receives fluorescence transmitted by a filter disposed in front of the photodetector.

Optionally, the system further includes:

a controller, and a scan head connected to the controller, a plurality of beamsplitters;

Each beam splitter corresponds to one laser, each laser is equipped with one detector, the number of beamsplitters, the number of lasers and the number of detectors are the same;

When the scanning head scans the capillary, the laser light output by each laser is sequentially irradiated on the capillary through a first optical fiber in the scanning head and a corresponding spectroscope of the laser, and the fluorescent dye in the capillary The excited fluorescence is transmitted to a second optical fiber reflected in the scanning head via a corresponding beam splitter of the laser to a detector provided to the laser;

The laser illumination from each laser output excites fluorescence at least one wavelength range on the capillary, and the fluorescence excited by each laser is received by a detector equipped with the laser.

Optional,

The at least two lasers are mounted within the scan head.

Optionally, the detector comprises:

a mirror, a plurality of filters, and a plurality of photodetectors, the number of the filters being the same as the number of photodetectors, each photodetector being mounted behind a filter;

The mirror reflects fluorescence received by the detector onto the plurality of filters;

Each filter transmits fluorescence of one wavelength range, and reflects fluorescence of other wavelength ranges to the mirror;

Each photodetector receives fluorescence transmitted by a filter disposed in front of the photodetector.

Optionally, the detector further includes:

a plurality of focusing mirrors, the number of focusing mirrors being the same as the number of photodetectors;

Each focusing mirror is placed in front of a photodetector to focus the fluorescence transmitted by a filter to a photodetector.

Optionally, characterized in that

The target substance to be tested includes protein molecules, polypeptide molecules, amino acid molecules, molecules of amino acid derivatives, alkaloid molecules, nitrogen-containing non-protein biomolecules, steroid molecules, sterol molecules, nucleotide molecules, and nucleus in body fluids. A glycoside derivative molecule, a lipid, a fatty acid derivative, a saccharide, a vitamin, a lipoprotein, an apolipoprotein, a glycoprotein, a mucin, a metalloprotein, or a glycolipid.

Optional,

The target substance to be tested includes DNA, and at least one of the biomass according to claim 7.

A capillary electrophoresis detection method, the method comprising:

Laser light output from at least two lasers is irradiated on the capillary, and each laser output has a different wavelength;

The fluorescent dye in the detection sample in the capillary is excited by the laser to generate fluorescence in a plurality of wavelength ranges, and the detection sample in the capillary includes a plurality of target substances to be tested, the target substance including the biological substance labeled with the fluorescent dye Or a biological substance that binds to an antibody labeled with a fluorescent dye;

The plurality of detectors receive fluorescence of a plurality of wavelength ranges, thereby detecting the kind or content of the plurality of target substances to be tested included in the detection sample in the capillary.

Optionally, the plurality of detectors receive fluorescence of a plurality of wavelength ranges, and further detecting the type or content of the plurality of target substances to be tested included in the detection sample in the capillary comprises:

Multiple detectors receive fluorescence in multiple wavelength ranges to simultaneously detect detection in the capillary The DNA included in the sample, and at least one species or content of the biomass according to claim 7.

According to the above technical solution, the present invention has the following beneficial effects:

The invention provides a capillary electrophoresis detection system and a detection method, at least two lasers, a capillary tube, the detection sample in the capillary tube comprises a plurality of target substances to be tested, and the target substance to be tested comprises a fluorescent dye-labeled substance. a biological substance or a biological substance to which an antibody labeled with a fluorescent dye is bound; a plurality of detectors; laser light output from the at least two lasers is irradiated on the capillary, and a fluorescent dye in the detection sample in the capillary is subjected to laser light The excitation generates fluorescence of a plurality of wavelength ranges, and the plurality of detectors receive fluorescence of a plurality of wavelength ranges, thereby detecting the kind or content of the substance contained in the detection sample in the capillary. At least two lasers are used to illuminate the capillary. Each laser outputs a different wavelength of laser light. The number of wavelengths of fluorescence that can be excited is greater than the wavelength range of fluorescence that a laser can excite. One wavelength range of fluorescence represents a kind of fluorescence. The target substance to be tested uses at least two lasers to detect more kinds of target substances to be detected than one laser, thereby improving the detection speed and saving the test sample.

DRAWINGS

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below. Obviously, the drawings in the following description are only It is a certain embodiment of the present invention, and other drawings can be obtained from those skilled in the art without any creative work.

1 is a schematic structural diagram of a capillary electrophoresis detection system according to an embodiment of the present invention;

2 is a schematic structural diagram of a capillary electrophoresis detection system according to an embodiment of the present invention;

3 is another schematic structural diagram of a capillary electrophoresis detection system according to an embodiment of the present invention;

4 is a schematic structural diagram of a detector according to an embodiment of the present invention;

FIG. 5 is a schematic structural diagram of another detector according to an embodiment of the present invention; FIG.

FIG. 6 is a flowchart of a capillary electrophoresis detection method according to an embodiment of the present invention.

detailed description

In order to provide an implementation scheme for simultaneously detecting a plurality of biological substances labeled with a fluorescent dye, an embodiment of the present invention provides a capillary electrophoresis detection system and method, and a preferred embodiment of the present invention will be described below with reference to the accompanying drawings.

In the prior art, a laser is used to excite the fluorescence generated by a sample in a capillary tube for detection. A laser can excite a limited range of wavelengths of fluorescence, and a wavelength range of fluorescence represents a target substance to be tested, that is, a laser. The type of target substance to be tested in the test sample that can be detected is limited, and only four or five kinds of biological substances can be detected at a time. In general, each test requires the detection of a large variety of biological substances, which requires multiple tests to complete. Not only does the time required for the test be very long, but a test sample is required for each test. The amount of test sample required is large, especially if the test sample is mostly blood, which will cause certain damage to the patient.

The present invention provides a system and method for simultaneously detecting a large variety of substances from a single test sample.

FIG. 1 is a schematic structural diagram of a capillary electrophoresis detection system according to an embodiment of the present invention, including:

At least two lasers 101, each having a different wavelength of laser output; a capillary 102, the detection sample in the capillary 102 comprising a plurality of target substances to be tested, the target substance to be detected comprising a biological substance labeled with a fluorescent dye or a biological substance combined with an antibody labeled with a fluorescent dye; a plurality of detectors 103;

The laser light output from the at least two lasers 101 is irradiated on the capillary 102, and the fluorescent dye in the detection sample in the capillary 102 is excited by the laser to generate fluorescence in a plurality of wavelength ranges, and the plurality of detectors 103 receive fluorescence detection in various wavelength ranges. The type or content of the substance contained in the test sample in the capillary. The fluorescent dye may be a fluorescent dye directly labeled on the target substance to be tested, or may be a fluorescent dye labeled on the antibody bound to the target substance to be tested.

The laser light output by each laser is irradiated onto the capillary 102 via mirror reflection or transmission of the optical fiber, transmitted through the capillary 102, and the fluorescent dye in the detection sample in the excitation capillary 102 fluoresces. Each laser outputs a different wavelength of laser light, and the range of wavelengths of fluorescence that can be excited is also different. Fluorescence in different wavelength ranges represents different types of target analytes in the sample detected in the capillary. The more the number of fluorescent wavelength ranges obtained by excitation, the more species that can be detected.

A wavelength laser can excite a limited range of wavelengths of fluorescence, using different wavelengths At least two lasers are capable of exciting a range of wavelengths of fluorescence that is greater than the range of wavelengths of fluorescence that a laser can excite. Fluorescence of different wavelength ranges represents different types of biological species, and thus the types of biological species that can be detected There are also many.

Capillary 102 employs a capillary having an inner diameter of from 5 micrometers to 100 micrometers, an outer diameter of from 50 micrometers to 300 micrometers, and a length of from 3 centimeters to 80 centimeters.

The capillary electrophoresis detection system detects at least two possible structures. The first possible structure, as shown in Figure 2, includes:

a filter, and a photodetector;

In a first possible configuration, each of the plurality of detectors includes a filter and a photodetector. A filter can only reflect fluorescence in a range of wavelengths, reflecting fluorescence in other wavelength ranges, and a photodetector disposed behind each filter is used to receive the fluorescence transmitted by the filter.

Taking FIG. 2 as an example, the filter m1 and the photodetector n1 are one detector, the filter m1 is disposed in front of the photodetector n1, and the photodetector n1 receives the fluorescence transmitted by the filter m1; the filter m2 And the photodetector n2 is a detector, the filter m2 is disposed in front of the photodetector n2, the photodetector n2 receives the fluorescence transmitted by the filter m2; the filter m3 and the photodetector n3 are a detector, The filter m3 is disposed before the photodetector n3, the photodetector n3 receives the fluorescence transmitted by the filter m3, and so on, the filter mi and the photodetector ni are one detector, and the filter mi is disposed at Before the photodetector ni, the photodetector ni receives the fluorescence transmitted by the filter mi. Where i is the number of wavelength ranges of fluorescence produced by the fluorescent dye in the test sample in at least two laser excitation capillaries.

A second possible structure, as shown in FIG. 3, the system further includes:

The controller 301 has a scan head 302 connected to the controller, and a plurality of beamsplitters.

Each beam splitter corresponds to one laser, each laser is equipped with a detector, the number of beamsplitters, the number of lasers and the number of detectors are the same.

When the scanning head 302 scans the capillary 102, the laser output from each laser passes through The first optical fiber in the scanning head 302 is sequentially irradiated on the capillary 102 through a spectroscope corresponding to the laser, and the fluorescent light excited by the fluorescent dye in the detecting sample 102 is reflected by the corresponding spectroscope of the laser to The second optical fiber in the scan head 302 is transmitted to a detector provided to the laser;

The scanning head 302 includes a plurality of beam splitters, a plurality of first fibers, and a plurality of second fibers. Each laser corresponds to a beam splitter, a first fiber, and a second fiber. The laser output from each laser is transmitted by the corresponding first optical fiber of the laser, and is irradiated onto the capillary 102 through a spectroscope corresponding to the laser. The capillary 102 detects the fluorescence excited by the fluorescent dye in the sample, and the corresponding spectroscopic light is passed through the laser. The mirror is reflected to a second fiber corresponding to the laser, and the second fiber transmits the received fluorescence to a detector provided to the laser.

It should be noted that the “first” in the first optical fiber and the “second” in the second optical fiber represent not the order but a type, and the first optical fiber is not an optical fiber but a type of optical fiber. The second fiber is not a fiber, but a type of fiber. The scanning head 302 has a plurality of first fibers, a plurality of second fibers, and the number of the first fibers. The number of the second fibers is the same as the number of the lasers. Each laser corresponds to a first fiber and a second fiber.

For example, as shown in FIG. 3, the laser light output by the laser a1 is transmitted by the first optical fiber x1 in the scanning head, and transmitted through the beam splitter y1 to be irradiated in the capillary 102. The fluorescence of the fluorescent dye excited by the detection sample of the capillary 102 is split. The mirror y1 is reflected to the second optical fiber z1 in the scan head, and the second optical fiber z1 transmits the fluorescence to the detector b1 provided to the laser a1; the laser output from the laser a2 is transmitted by the first optical fiber x2 in the scanning head, and transmitted through the beam splitter y2 Irradiated in the capillary 102, the fluorescence of the fluorescent dye excited in the detection sample of the capillary 102 is reflected by the beam splitter y2 to the second optical fiber z2 in the scanning head, and the second optical fiber z2 transmits the fluorescence to the detector b2 provided to the laser a2. The laser outputted by the laser a3 is transmitted by the first optical fiber x3 in the scanning head, and transmitted through the beam splitter y3 to be irradiated in the capillary 102. The fluorescent light excited by the fluorescent dye in the detection sample of the capillary 102 is reflected by the spectroscope y3 to the scanning head. The second fiber z3 transmits the fluorescence from the second fiber z3 to the detector b3 provided to the laser a3; the laser output from the laser a4 is transmitted by the first fiber x4 in the scanning head, transmitted through Y4 irradiating light microscope in the capillary 102, the capillary test sample a fluorescent dye 102 is excited by a fluorescence spectroscope y4 Reflected to the second fiber z4 in the scan head, the second fiber z4 transmits the fluorescence to the detector b4 provided to the laser a4.

In practical applications, the number of lasers and detectors can be specifically set according to actual needs, and is not limited to the four lasers and four detectors shown in FIG. 3, and the number of lasers and detectors can be the same.

In one example, at least two lasers are mounted in scan head 302. Of course, at least two lasers can also be mounted outside of the scanning head 302. A plurality of detectors can be mounted outside of the scanning head 302 and can also be mounted within the scanning head 302. The technician can set it according to the actual needs.

The controller 301 controls the scanning head 302 to scan the capillary 102 such that the laser light output by each laser is irradiated onto the capillary 102 one by one, and the fluorescence generated by the excitation of the fluorescent dye in the detection sample in the capillary 102 is received. The direction in which the scanning head 302 scans is as shown in FIG. It should be noted that the scanning direction of the scanning head 302 can also be opposite to the scanning direction shown in FIG. 3, and the technician can set it according to actual needs.

In a second possible implementation, in one example, as shown in FIG. 4, the detector includes:

In a capillary electrophoresis detection system, there are multiple detectors, each of which includes a mirror, a plurality of filters, and a plurality of photodetectors. A laser can excite fluorescence in several wavelength ranges, and the detector equipped with the laser receives fluorescence in several wavelength ranges that are excited. The detector includes a mirror that reflects the fluorescence received by the detector to the filter. Each filter transmits fluorescence in one wavelength range and reflects fluorescence in other wavelength ranges back to the mirror. A photodetector is disposed behind each filter, and the photodetector receives only fluorescence of a wavelength range transmitted by the filter. In turn, the detector receives fluorescence in all wavelength ranges excited by a laser. Also, in order to reduce the loss of light energy, each filter is placed in close contact with the mirror.

Taking FIG. 4 as an example, the detector includes a mirror 401, four filters p1 to p4, and four photodetectors q1 to q4. The number of filters is the same as the number of photodetectors.

The mirror 401 is large and covers all of the filters p1 to p4, and each of the filters p1 to p4 is placed in close contact with the mirror 501. The received fluorescence is reflected by the mirror 401 to each of the filters p1 to p4. The fluorescence transmitted by the filter p1 is received by the photodetector q1, the fluorescence transmitted by the filter p2 is received by the photodetector q2, and the fluorescence transmitted by the filter p3 is received by the photodetector q3, and the filter is filtered. The fluorescence transmitted by the sheet p4 is received by the photodetector q4.

In practical applications, the number of filters and photodetectors provided in each detector is not less than the number of wavelengths of fluorescence that a laser can excite. In general, a laser can excite fluorescence in four or five wavelength ranges, and four or five filters and four or five photodetectors can be placed in one detector.

The photodetector includes a charge-coupled device (CCD) image sensor, an Avalanche Photo Diode (APD), a Complementary Metal Oxide Semiconductor (CMOS) image sensor, and a photomultiplier tube ( Photo Multiplier Tube, PMT).

In a second possible implementation manner, in an example, as shown in FIG. 5, the detector further includes:

A focusing mirror is added in front of each photodetector that focuses the fluorescence transmitted by one filter to the photodetector. As shown in FIG. 5, the focusing mirror L1 focuses the fluorescence transmitted by the filter p1 to the photodetector q1; the focusing mirror L2 focuses the fluorescence transmitted by the filter p2 to the photodetector q2; the focusing mirror L3 filters the light. The fluorescence transmitted by the sheet p3 is focused to the photodetector q3; the focusing mirror L4 focuses the fluorescence transmitted by the filter p4 to the photodetector q4.

In one example, the target substance to be tested includes protein molecules, polypeptide molecules, amino acid molecules, molecules of amino acid derivatives, alkaloid molecules, nitrogen-containing non-protein biomolecules, steroid molecules, sterol molecules, and nucleus in body fluids. Glycosylate molecule, nucleotide derivative molecule, lipid, fatty acid derivative Any one or more of substances, sugars, vitamins, lipoproteins, apolipoproteins, glycoproteins, mucins, metalloproteins, glycolipids.

In another example, the target substance to be tested includes DNA, protein molecules in a body fluid, polypeptide molecules, amino acid molecules, molecules of amino acid derivatives, alkaloid molecules, nitrogen-containing non-protein biomolecules, steroid molecules, sterols Any of a class of molecules, nucleotide molecules, nucleotide derivative molecules, lipids, fatty acid derivatives, sugars, vitamins, lipoproteins, apolipoproteins, glycoproteins, mucins, metalloproteins, glycolipids Or a variety.

The capillary electrophoresis detection system provided by the invention realizes the simultaneous detection of DNA and non-DNA biological substances in the same test sample, and unifies genetic detection, clinical biochemical detection and immunological detection, and has a major breakthrough in clinical diagnosis. .

As can be seen from the above, the present invention has the following beneficial effects:

At least two lasers are used to illuminate the capillary. Each laser outputs a different wavelength of laser light. The number of wavelengths of fluorescence that can be excited is greater than the wavelength range of fluorescence that a laser can excite. One wavelength range of fluorescence represents a kind of fluorescence. The target substance to be tested uses at least two kinds of lasers to detect more kinds of biological substances than one laser, which improves the detection speed and saves the test sample.

FIG. 6 is a flowchart of a capillary electrophoresis detection method according to an embodiment of the present invention, including:

601: Laser light output from at least two lasers is irradiated on the capillary, and each laser output has a different wavelength.

602: The fluorescent dye in the detection sample in the capillary is excited by the laser to generate fluorescence of a plurality of wavelength ranges, the detection sample in the capillary includes a plurality of target substances to be tested, and the target substance to be tested includes a fluorescent dye-labeled substance. Biological material or biological substance that binds to an antibody labeled with a fluorescent dye.

603: The plurality of detectors receive fluorescence of a plurality of wavelength ranges, thereby detecting the kind or content of the plurality of target substances to be tested included in the detection sample in the capillary.

In one example, a plurality of detectors receive fluorescence in a plurality of wavelength ranges, thereby detecting protein molecules, polypeptide molecules, amino acid molecules, molecules of amino acid derivatives, alkaloid molecules, nitrogen-containing molecules in the test sample in the capillary. Non-protein biomolecules, steroid molecules, sterol molecules, nucleotide molecules, nucleotide derivative molecules, lipids, fatty acid derivatives, sugars, vitamins, lipoproteins, Any one or more of lipoprotein, glycoprotein, mucin, metalloprotein, glycolipid.

In one example, a plurality of detectors receive fluorescence in a plurality of wavelength ranges, and simultaneously detect DNA labeled with a fluorescent dye in the detection sample in the capillary, and molecules of a protein molecule, a polypeptide molecule, an amino acid molecule, and an amino acid derivative, Alkaloid molecules, nitrogen-containing non-protein biomolecules, steroid molecules, sterol molecules, nucleotide molecules, nucleotide derivative molecules, lipids, fatty acid derivatives, sugars, vitamins, lipoproteins, apolipoproteins, Any one or more of glycoprotein, mucin, metalloprotein, and glycolipid.

The detector receives fluorescence in multiple wavelength ranges and is capable of detecting the wavelength range of the received fluorescence. By comparing with the preset test standards, it is possible to know which type of biological substance is contained in the test sample and the content of the substance.

The capillary electrophoresis detection method shown in FIG. 6 is a method corresponding to the capillary electrophoresis detection system shown in FIG. 1 to FIG. 5. The specific implementation manner is described in the system shown in FIG. 1 to FIG. 5, and details are not described herein again.

The above description is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can also make several improvements and retouchings without departing from the principles of the present invention. It should be considered as the scope of protection of the present invention.

Claims

A capillary electrophoresis detection system, characterized in that the system comprises:

At least two lasers each having a different wavelength of the output; a capillary tube, the detection sample in the capillary tube comprising a plurality of target substances to be tested, the target substance to be tested comprising a biological substance labeled with a fluorescent dye or a labeled substance a biological substance of an antibody to a fluorescent dye; a plurality of detectors;

The laser light output by the at least two lasers is irradiated on the capillary, and the fluorescent dye in the detection sample in the capillary is excited by the laser to generate fluorescence of a plurality of wavelength ranges, and the plurality of detectors receive a plurality of wavelength ranges Fluorescence, which in turn detects the type or content of a plurality of target substances to be tested included in the test sample in the capillary.
The system of claim 1 wherein each detector comprises:

a filter, and a photodetector;

Each filter transmits fluorescence in a range of wavelengths, and each photodetector receives fluorescence transmitted by a filter disposed in front of the photodetector.
The system of claim 1 wherein the system further comprises:

a controller, and a scan head connected to the controller, a plurality of beamsplitters;

Each beam splitter corresponds to one laser, each laser is equipped with one detector, the number of beamsplitters, the number of lasers and the number of detectors are the same;

When the scanning head scans the capillary, the laser light output by each laser is sequentially irradiated on the capillary through a first optical fiber in the scanning head and a corresponding spectroscope of the laser, and the fluorescent dye in the capillary The excited fluorescence is transmitted to a second optical fiber reflected in the scanning head via a corresponding beam splitter of the laser to a detector provided to the laser;

The laser illumination from each laser output excites fluorescence at least one wavelength range on the capillary, and the fluorescence excited by each laser is received by a detector equipped with the laser.
The system of claim 3 wherein:

The at least two lasers are mounted within the scan head.
The system of claim 3 wherein said detector comprises:

a mirror, a plurality of filters, and a plurality of photodetectors, the number of the filters and photodetection The number of detectors is the same, and each photodetector is mounted behind a filter;

The mirror reflects fluorescence received by the detector onto the plurality of filters;

Each filter transmits fluorescence of one wavelength range, and reflects fluorescence of other wavelength ranges to the mirror;

Each photodetector receives fluorescence transmitted by a filter disposed in front of the photodetector.
The system of claim 5 wherein said detector further comprises:

a plurality of focusing mirrors, the number of focusing mirrors being the same as the number of photodetectors;

Each focusing mirror is placed in front of a photodetector to focus the fluorescence transmitted by a filter to a photodetector.
A system according to any one of claims 1-6, wherein

The target substance to be tested includes protein molecules, polypeptide molecules, amino acid molecules, molecules of amino acid derivatives, alkaloid molecules, nitrogen-containing non-protein biomolecules, steroid molecules, sterol molecules, nucleotide molecules, and nucleus in body fluids. A glycoside derivative molecule, a lipid, a fatty acid derivative, a saccharide, a vitamin, a lipoprotein, an apolipoprotein, a glycoprotein, a mucin, a metalloprotein, or a glycolipid.
The system of claim 7 wherein:

The target substance to be tested includes DNA, and at least one of the biomass according to claim 7.
A capillary electrophoresis detection method, characterized in that the method comprises:

Laser light output from at least two lasers is irradiated on the capillary, and each laser output has a different wavelength;

The fluorescent dye in the detection sample in the capillary is excited by the laser to generate fluorescence in a plurality of wavelength ranges, and the detection sample in the capillary includes a plurality of target substances to be tested, the target substance including the biological substance labeled with the fluorescent dye Or a biological substance that binds to an antibody labeled with a fluorescent dye;

The plurality of detectors receive fluorescence of a plurality of wavelength ranges, thereby detecting the kind or content of the plurality of target substances to be tested included in the detection sample in the capillary.