CN109632741A - A kind of detection method of composite fluorescence microballoon reagent card - Google Patents
A kind of detection method of composite fluorescence microballoon reagent card Download PDFInfo
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- CN109632741A CN109632741A CN201811608777.0A CN201811608777A CN109632741A CN 109632741 A CN109632741 A CN 109632741A CN 201811608777 A CN201811608777 A CN 201811608777A CN 109632741 A CN109632741 A CN 109632741A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The invention discloses a kind of detection methods of composite fluorescence microballoon reagent card, successively as follows including step: S1: first light source emits light beam and passes through the first optical filter, transmit the light of most of first light source wavelength, light beam is under the action of semi-transparent semi-reflecting two to color separation film a, reflect the light of most of first light source wavelength, change beam direction, it is irradiated on sample to be tested under the convergence of convex lens, illuminated sample to be tested is under light beam irradiation, emitting fluorescence has diversity, is irradiated to semi-transparent semi-reflecting two to color separation film a by the convergence of convex lens；The present invention, entire light path design greatly improves the launching efficiency of transmitting light, improve the precision of fluorescence detection, the detection to composite fluorescence microballoon reagent card is realized simultaneously, different subject matter concentration informations, while the wavelength of the unlimited optical device of this light path design are obtained, according to the spectral characteristic of different composite fluorescence microballoons, device parameters can be replaced, which has universality.
Description
Technical field
The present invention relates to the field of optical detecting channel design, specially a kind of detection sides of composite fluorescence microballoon reagent card
Method.
Background technique
Currently, immunochromatography (lateral flow immunoassay, LFIA) Fast Detection Technique is built upon chromatography
Technology on the basis of technology and Ag-Ab specific immune response.Fluorescence immune chromatography test paper bar test strips are made with fluorchrome
For marker, in situ quantitation detection can be widely applied to, be the important directions of the following development of detection technique immediately.
But the detection method of existing composite fluorescence microballoon reagent card has the following deficiencies: the launching efficiency of transmitting light
Low, the precision of fluorescence detection is low, does not have universality；For these defects, so we design a kind of composite fluorescence microballoon examination
The detection method of agent card is necessary.
Summary of the invention
The purpose of the present invention is to provide a kind of detection method of composite fluorescence microballoon reagent card, entire light path design is very big
Ground improves the launching efficiency of transmitting light, improves the precision of fluorescence detection, while realizing the inspection to composite fluorescence microballoon reagent card
It surveys, different subject matter concentration informations, while the wavelength of the unlimited optical device of this light path design is obtained, according to different composite fluorescences
The spectral characteristic of microballoon can replace device parameters, which has universality.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of detection method of composite fluorescence microballoon reagent card, including step are successively as follows:
S1: first light source emits light beam by the first optical filter, transmits the light of most of first light source wavelength, light beam is half
Saturating half anti-two, to the light under the action of color separation film a, reflecting most of first light source wavelength, changes beam direction, in convex lens
It is irradiated on sample to be tested under convergence, illuminated sample to be tested is under light beam irradiation, and transmitting fluorescence has diversity, through excess convexity
It is glimmering greater than 420nm to color separation film a transmission peak wavelength to color separation film a, semi-transparent semi-reflecting two that the convergence of lens is irradiated to semi-transparent semi-reflecting two
Light, while reflecting the interference light of 365nm wavelength；
Wherein, the first light source is the point light source for emitting the directional light of 365nm；
S2: second light source emits light beam by the second optical filter, transmits the light of most of second light source wavelength, light beam is half
Saturating half anti-two, to the light under the action of color separation film b, reflecting most of second light source wavelength, changes beam direction, in convex lens
It is irradiated on sample to be tested under convergence；
Wherein, the second light source is the point light source for emitting the directional light of 470nm；
S3: after most of Fluoroscopic under the action of semi-transparent semi-reflecting two to color separation film b, reflecting wavelength is 470nm's
Light is interfered, and has transmitted the fluorescence that wavelength is greater than 510；
S4: light beam after transmission reflects the fluorescence that wavelength is 525nm under the action of semi-transparent semi-reflecting two to color separation film c,
The wavelength after 525nm third optical filter, collected, and transmitted by photodiode a is greater than the fluorescence of 610nm, passes through
After the 4th optical filter of 610nm, collected by photodiode b；
Wherein, first light source is indicated with LED1；Second light source is indicated with LED2；Semi-transparent semi-reflecting two to color separation film a M1 table
Show；Semi-transparent semi-reflecting two are indicated to color separation film b with M2；Semi-transparent semi-reflecting two are indicated to color separation film c with M3；First optical filter F1 table
Show；Second optical filter is indicated with F2；Third optical filter is indicated with F3；4th optical filter is indicated with F4；Photodiode a PDl
It indicates；Photodiode b is indicated with PD2；Convex lens is indicated with LEN；Sample to be tested is indicated with OBJ.
As a further solution of the present invention: described semi-transparent semi-reflecting two to color separation film a model parameter be R365-T420, institute
It is R470-T510 that semi-transparent semi-reflecting two, which are stated, to color separation film b model parameter, and described semi-transparent semi-reflecting two are to color separation film c model parameter
R525-T610。
As a further solution of the present invention: the photodiode a and photodiode b is for collecting two kinds not
With the different fluorescence of fluorescent microsphere transmitting, what the photodiode a was collected into is the 525nm fluorescence of XC microballoon transmitting, described
Photodiode b be collected into be europium microballoon transmitting 610nm fluorescence.
Beneficial effects of the present invention: as follows, S1: first light source emits light beam and passes through the first optical filter, transmission
The light of most of first light source wavelength, light beam reflect most of first light source wave under the action of semi-transparent semi-reflecting two to color separation film a
Long light changes beam direction, is irradiated on sample to be tested under the convergence of convex lens, and illuminated sample to be tested shines in light beam
It penetrates down, transmitting fluorescence has diversity, semi-transparent semi-reflecting two are irradiated to color separation film a by the convergence of convex lens, semi-transparent semi-reflecting two
It is greater than the fluorescence of 420nm to color separation film a transmission peak wavelength, while reflects the interference light of 365nm wavelength wherein, first light source is transmitting
The point light source of the directional light of 365nm；S2: second light source emits light beam by the second optical filter, transmits most of second light source wave
Long light, light beam reflect the light of most of second light source wavelength, change light beam under the action of semi-transparent semi-reflecting two to color separation film b
Direction is irradiated on sample to be tested under the convergence of convex lens；Wherein, second light source is the point light for emitting the directional light of 470nm
Source；S3: after most of Fluoroscopic under the action of semi-transparent semi-reflecting two to color separation film b, the interference that wavelength is 470nm is reflected
Light, and transmitted the fluorescence that wavelength is greater than 510；S4: light beam after transmission is under the action of semi-transparent semi-reflecting two to color separation film c, instead
The wavelength that the fluorescence that wavelength is 525nm is collected by photodiode a after 525nm third optical filter, and transmitted is penetrated
Fluorescence greater than 610nm is collected after the 4th optical filter of 610nm by photodiode b；Wherein photodiode a is collected into
Be XC microballoon transmitting 525nm fluorescence, photodiode b be collected into be europium microballoon transmitting 610nm fluorescence；The process, it is whole
A light path design greatly improves the launching efficiency of transmitting light, improves the precision of fluorescence detection, while realizing to composite fluorescence
The detection of microballoon reagent card obtains different subject matter concentration informations, while the wavelength of the unlimited optical device of this light path design, according to
The spectral characteristic of different composite fluorescence microballoons, can replace device parameters, which has universality.
Detailed description of the invention
In order to facilitate the understanding of those skilled in the art, the present invention will be further described below with reference to the drawings.
Fig. 1 is optical detection exciting light index path of the present invention；
Fig. 2 is that optical detection of the present invention emits light index path.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff all other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
As shown in Figs. 1-2, a kind of detection method of composite fluorescence microballoon reagent card, including step are successively as follows:
S1: first light source emits light beam by the first optical filter, transmits the light of most of first light source wavelength, light beam is half
Saturating half anti-two, to the light under the action of color separation film a, reflecting most of first light source wavelength, changes beam direction, in convex lens
It is irradiated on sample to be tested under convergence, illuminated sample to be tested is under light beam irradiation, and transmitting fluorescence has diversity, through excess convexity
It is glimmering greater than 420nm to color separation film a transmission peak wavelength to color separation film a, semi-transparent semi-reflecting two that the convergence of lens is irradiated to semi-transparent semi-reflecting two
Light, while reflecting the interference light of 365nm wavelength；
Wherein, first light source is the point light source for emitting the directional light of 365nm；
S2: second light source emits light beam by the second optical filter, transmits the light of most of second light source wavelength, light beam is half
Saturating half anti-two, to the light under the action of color separation film b, reflecting most of second light source wavelength, changes beam direction, in convex lens
It is irradiated on sample to be tested under convergence；
Wherein, second light source is the point light source for emitting the directional light of 470nm；
S3: after most of Fluoroscopic under the action of semi-transparent semi-reflecting two to color separation film b, reflecting wavelength is 470nm's
Light is interfered, and has transmitted the fluorescence that wavelength is greater than 510；
Light beam after S4 transmission reflects the fluorescence that wavelength is 525nm under the action of semi-transparent semi-reflecting two to color separation film c,
The wavelength after 525nm third optical filter, collected, and transmitted by photodiode a is greater than the fluorescence of 610nm, passes through
After the 4th optical filter of 610nm, collected by photodiode b；
Wherein, first light source is indicated with LED1；Second light source is indicated with LED2；Semi-transparent semi-reflecting two to color separation film a M1 table
Show；Semi-transparent semi-reflecting two are indicated to color separation film b with M2；Semi-transparent semi-reflecting two are indicated to color separation film c with M3；First optical filter F1 table
Show；Second optical filter is indicated with F2；Third optical filter is indicated with F3；4th optical filter is indicated with F4；Photodiode a PD1
It indicates；Photodiode b is indicated with PD2；Convex lens is indicated with LEN；Sample to be tested is indicated with OBJ；
Wherein, semi-transparent semi-reflecting two be R365-T420 to color separation film a model parameter, and semi-transparent semi-reflecting two join to color separation film b model
Number be R470-T510, semi-transparent semi-reflecting two to color separation film c model parameter be R525-T610, photodiode a and photodiode b
For collecting the different fluorescence of two kinds of different fluorescent microsphere transmittings, that photodiode a is collected into is the 525nm of XC microballoon transmitting
Fluorescence, photodiode b be collected into be europium microballoon transmitting 610nm fluorescence.
The working principle of the invention: as follows, S1: first light source emits light beam and passes through the first optical filter, transmission
The light of most of first light source wavelength, light beam reflect most of first light source wave under the action of semi-transparent semi-reflecting two to color separation film a
Long light changes beam direction, is irradiated on sample to be tested under the convergence of convex lens, and illuminated sample to be tested shines in light beam
It penetrates down, transmitting fluorescence has diversity, semi-transparent semi-reflecting two are irradiated to color separation film a by the convergence of convex lens, semi-transparent semi-reflecting two
It is greater than the fluorescence of 420nm to color separation film a transmission peak wavelength, while reflects the interference light of 365nm wavelength；Wherein, first light source is hair
Penetrate the point light source of the directional light of 365nm；S2: second light source emits light beam by the second optical filter, transmits most of second light source
The light of wavelength, light beam reflect the light of most of second light source wavelength, change light under the action of semi-transparent semi-reflecting two to color separation film b
Shu Fangxiang is irradiated on sample to be tested under the convergence of convex lens；Wherein, second light source is the point for emitting the directional light of 470nm
Light source；S3: after most of Fluoroscopic under the action of semi-transparent semi-reflecting two to color separation film b, the interference that wavelength is 470nm is reflected
Light, and transmitted the fluorescence that wavelength is greater than 510；S4: light beam after transmission is under the action of semi-transparent semi-reflecting two to color separation film c, instead
The wavelength that the fluorescence that wavelength is 525nm is collected by photodiode a after 525nm third optical filter, and transmitted is penetrated
Fluorescence greater than 610nm is collected after the 4th optical filter of 610nm by photodiode b；Wherein photodiode a is collected into
Be XC microballoon transmitting 525nm fluorescence, photodiode b be collected into be europium microballoon transmitting 610nm fluorescence；The process, it is whole
A light path design greatly improves the launching efficiency of transmitting light, improves the precision of fluorescence detection, while realizing to composite fluorescence
The detection of microballoon reagent card obtains different subject matter concentration informations, while the wavelength of the unlimited optical device of this light path design, according to
The spectral characteristic of different composite fluorescence microballoons, can replace device parameters, which has universality.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (3)
1. a kind of detection method of composite fluorescence microballoon reagent card, which is characterized in that successively as follows including step:
S1: first light source emits light beam by the first optical filter, transmits the light of most of first light source wavelength, light beam is semi-transparent half
Anti- two, to the light under the action of color separation film a, reflecting most of first light source wavelength, change beam direction, in the convergence of convex lens
Under be irradiated on sample to be tested, illuminated sample to be tested light beam irradiation under, transmitting fluorescence have diversity, by convex lens
Convergence be irradiated to semi-transparent semi-reflecting two to color separation film a, semi-transparent semi-reflecting two are greater than the fluorescence of 420nm to color separation film a transmission peak wavelength, together
When reflect 365nm wavelength interference light；
Wherein, the first light source is the point light source for emitting the directional light of 365nm；
S2: second light source emits light beam by the second optical filter, transmits the light of most of second light source wavelength, light beam is semi-transparent half
Anti- two, to the light under the action of color separation film b, reflecting most of second light source wavelength, change beam direction, in the convergence of convex lens
Under be irradiated on sample to be tested；
Wherein, the second light source is the point light source for emitting the directional light of 470nm；
S3: after most of Fluoroscopic under the action of semi-transparent semi-reflecting two to color separation film b, the interference that wavelength is 470nm is reflected
Light, and transmitted the fluorescence that wavelength is greater than 510；
Light beam after S4 transmission reflects the fluorescence that wavelength is 525nm, passes through under the action of semi-transparent semi-reflecting two to color separation film c
The wavelength after 525nm third optical filter, collected, and transmitted by photodiode a is greater than the fluorescence of 610nm, by 610nm the
After four optical filters, collected by photodiode b；
Wherein, first light source is indicated with LED1；Second light source is indicated with LED2；Semi-transparent semi-reflecting two are indicated to color separation film a with M1；Half
Saturating half anti-two is indicated to color separation film b with M2；Semi-transparent semi-reflecting two are indicated to color separation film c with M3；First optical filter is indicated with F1；Second
Optical filter is indicated with F2；Third optical filter is indicated with F3；4th optical filter is indicated with F4；Photodiode a is indicated with PD1；Light
Electric diode b is indicated with PD2；Convex lens is indicated with LEN；Sample to be tested is indicated with OBJ.
2. a kind of detection method of composite fluorescence microballoon reagent card according to claim 1, which is characterized in that described semi-transparent
Half anti-two to color separation film a model parameter be R365-T420, described semi-transparent semi-reflecting two to color separation film b model parameter be R470-
T510, described semi-transparent semi-reflecting two to color separation film c model parameter be R525-T610.
3. a kind of detection method of composite fluorescence microballoon reagent card according to claim 1, which is characterized in that the photoelectricity
The diode a and photodiode b is used to collect the different fluorescence of two kinds of different fluorescent microsphere transmittings, the photodiode
What a was collected into is the 525nm fluorescence of XC microballoon transmitting, and it is that the 610nm that europium microballoon emits is glimmering that the photodiode b, which is collected into,
Light.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855508A (en) * | 2020-07-22 | 2020-10-30 | 天津凌视科技有限公司 | Liquid detection device and liquid detection method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614367A (en) * | 2015-02-10 | 2015-05-13 | 华中科技大学 | Multi-channel optical detection system |
| CN104880450A (en) * | 2015-06-18 | 2015-09-02 | 苏州和迈精密仪器有限公司 | Line focusing detection system for immunofluorescence reagent card |
| CN105092544A (en) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | Optical excitation and detection system of fluorescent quantitative PCR detector |
| WO2018000664A1 (en) * | 2016-06-29 | 2018-01-04 | 山东科立森生物股份有限公司 | Capillary electrophoresis detection system and detection method |
| CN207396349U (en) * | 2017-07-11 | 2018-05-22 | 扬州千代科技有限公司 | A kind of colloidal gold chromatographic card interpretoscope |
-
2018
- 2018-12-27 CN CN201811608777.0A patent/CN109632741A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105092544A (en) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | Optical excitation and detection system of fluorescent quantitative PCR detector |
| CN104614367A (en) * | 2015-02-10 | 2015-05-13 | 华中科技大学 | Multi-channel optical detection system |
| CN104880450A (en) * | 2015-06-18 | 2015-09-02 | 苏州和迈精密仪器有限公司 | Line focusing detection system for immunofluorescence reagent card |
| WO2018000664A1 (en) * | 2016-06-29 | 2018-01-04 | 山东科立森生物股份有限公司 | Capillary electrophoresis detection system and detection method |
| CN207396349U (en) * | 2017-07-11 | 2018-05-22 | 扬州千代科技有限公司 | A kind of colloidal gold chromatographic card interpretoscope |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855508A (en) * | 2020-07-22 | 2020-10-30 | 天津凌视科技有限公司 | Liquid detection device and liquid detection method |
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