CN103575712A - Particle fluorescence detection wavelength instant configuration beam splitting system - Google Patents
Particle fluorescence detection wavelength instant configuration beam splitting system Download PDFInfo
- Publication number
- CN103575712A CN103575712A CN201310478988.8A CN201310478988A CN103575712A CN 103575712 A CN103575712 A CN 103575712A CN 201310478988 A CN201310478988 A CN 201310478988A CN 103575712 A CN103575712 A CN 103575712A
- Authority
- CN
- China
- Prior art keywords
- acousto
- fluorescence
- tunable filter
- camera lens
- optic tunable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a particle fluorescence detection wavelength instant configuration beam splitting system which comprises a laser light source and an optical shaping unit, wherein the optical shaping unit is arranged in front of the laser light source; a fluid forming unit is arranged in front of the optical shaping unit; an acousto-optic tunable filter is arranged in front of the fluid forming unit and is connected with a radio frequency driver; the fluid forming unit comprises sheath fluid, a sample, particles and a particle detection area. According to the technical scheme, optional selection of central wavelength, bandwidth control of a fluorescence channel and modulation of fluorescence transmittance in an operating range of the acousto-optic tunable filter are realized in a particle fluorescence detection process according to the dye types and a to-be-detected target fluorescence wavelength channel needing to be dynamically configured; the defect that the fluorescence detection beam-splitting channel in conventional particle fluorescence detection beam-splitting structures such as a flow cytometry is relatively fixed and is difficult to adjust is overcome; the system can be unique or is combined with an optical filter beam-splitting method to serve as a beam-splitting scheme for particle fluorescence detection.
Description
Technical field
The present invention relates to fluorescence analysis method and the device field of sample, be specifically related to a kind of microparticle fluorescence detection wavelength and immediately configure beam splitting system.
Background technology
Fluorescence analysis method is usually used in the research of cell biology.In the method as there is multi parameter analysis and the discriminating that stronger wavelength resolution ability contributes to multiple dyestuff.Because different fluorescent dye applications is different, conventionally adopt multiple dyestuff collocation to use and can analyze preferably surface antibody, nucleotide sequence and albumen kind, so adopt the detection channels of capable of dynamic configuration wavelength of fluorescence parameter to contribute to system to adapt to different kind of dyes, obtain better analysis result.
Above-mentioned fluorescence analysis method is typically applied in flow cytometric analysis.Flow cytometric analysis is a kind of high velocity particle sample analysis process, by the method for the scattered light of measurement target particle scattering light source and the optical parametric evaluating objects particle characteristics such as fluorescence of dyestuff stimulated emission.Flow cytometry system conventionally by fluid forming unit 1100, fluorescence excitation unit 1300, light, collects the hardware such as minute photo detecting unit 1200 and computer data process software forms.
During flow cytometry system works, the target detection things such as microballoon, cell form streaming and focus on and pass through detection of particulates district in fluid forming unit.In detection zone, each particle is come from the multi-wavelength excitation irradiation of a plurality of light sources conventionally, forms scattered light and fluorescence signal.Light signal is collected, is separated to different parameters passage by light collection minute photo detecting unit and exports data handling system, final analysis Output rusults to by detector conversion.
The target detection such as microballoon, cell thing conventionally need be by material markings such as fluorescence probes, for helping detection and identify detected target.The fluorescent dye kind, the concentration that due to the different component of multiple different particle or particle of the same race, are colored are different.Therefore, these target particles can be measured simultaneously, and identify by spectral composition and intensity.Traditional flow cytometry system has configured numerous fluorescence parameter passages in order to possess the ability of the different fluorescence kinds of identification, it is reported that its fluorescence parameter passage configuring for surveying variety classes dyestuff of maximum systems has reached 17 kinds more than.But due to the light splitting scheme of conventional fluorescent parameter channel be use dead center wavelength and bandwidth filter system to realize different fluorescence separated.Conventionally the filter system of each fluorescence parameter passage is comprised of two parts, and first is two colo(u)r filters, and the fluorescence that upper one group of two colo(u)r filter transmission can be come is separated into two parts according to its light-filtering characteristic, the reflection of an a part of transmission part.Second portion is narrow band pass filter, selects to see through the fluorescence matching with specific objective dye fluorescence.As the characteristic that sees through of PE dyestuff detection channels can be 585 nm+/ _ 20nm, wherein 585nm is the characteristic peaks wavelength of PE dyestuff.Parameter characteristic due to optical filter, once manufactured, its optical characteristics is just fixing, therefore flow cytometry system is in order to possess the detectivity of multiple fluorescence, need possess more fluorescence detection parameter channel, once and each group fluorescence parameter passage configuration be fixed up, just must change optical filter allocation plan and change optical filter as need change kind and the bandwidth that passage fluorescence passes through.
Summary of the invention
The object of the invention is to overcome the problem that prior art exists, provide a kind of microparticle fluorescence to detect wavelength and immediately configure beam splitting system.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the present invention is achieved through the following technical solutions:
A kind of microparticle fluorescence detects wavelength and immediately configures beam splitting system excitation source, optical shaping unit and acousto-optic tunable filter, before described excitation source, be provided with optical shaping unit, described optical shaping is provided with fluid forming unit before unit, before described fluid forming unit, be provided with acousto-optic tunable filter, described acousto-optic tunable filter is connected with radio driver, and described fluid method of forming unit comprises sheath fluid, sample, particulate and detection of particulates district.
Further, be provided with dispersion element before described acousto-optic tunable filter, described dispersion element be provided with detector above.
Further, between described fluid forming unit and described acousto-optic tunable filter, be provided with phosphor collection camera lens.
Further, before described detector, be provided with the first positive light coke camera lens.
Further, between described phosphor collection camera lens and described acousto-optic tunable filter, be provided with the second positive light coke camera lens.
Further, between described the first positive light coke camera lens and described dispersion element, be provided with the first collimation camera lens.
Further, between described acousto-optic tunable filter and described dispersion element, be provided with the second collimation camera lens.
Further, described acousto-optic tunable filter is as core parts, and the wavelength coverage that described acousto-optic tunable filter covers and configuration quantity and IO interface are that the free type of air transmission and the optical-fiber type of Optical Fiber Transmission can be selected according to target wavelength characteristic.
Preferably, described radio driver possesses and changes radio-frequency (RF) driving signal frequency and realize the ability that fluorescence parameter passage dynamically changes centre wavelength and bandwidth; The radio-frequency (RF) driving signal of described radio driver can be single center frequency signal, can be also the stack of a plurality of independently different center frequency signals, and described center frequency value depends on the centre wavelength of target detection fluorescence; Described radio-frequency (RF) driving signal is the signal with certain bandwidth being worth centered by centre frequency.
Further, described dispersion element dispersion, by the target fluorescence of described acousto-optic tunable filter deflection output, can adopt grating or prism, and described grating can be reflection grating or transmission grating.
Further, described phosphor collection camera lens be high-NA and typical N A value should be greater than 0.6, described phosphor collection camera lens possesses collimation property, and the fluorescence excitation of tested particle is converted into approximate directional light, and the dispersion angle of described approximate directional light should be less than 0.5 degree.
Preferably, target detection fluorescence after described dispersion element dispersion through through described the first positive light coke lens focus in described detector, can make system adapt to because input radio frequency signal band is wider, and described acousto-optic tunable filter output wavelength wider width, the problem that dispersion rear space position is wider, described detector is collected the fluorescence of wider spectrum.
Further, target detection fluorescence changes into directional light through described the first collimation camera lens after described dispersion element, and then described the first positive light coke lens focus of process is in described detector.
Further, the target detection fluorescence that converts approximate directional light to by described phosphor collection camera lens, can reduce the acousto-optic interaction causing due to the sound transit time of hot spot and postpone through described the second positive light coke lens focus in described acousto-optic tunable filter.
Further, described dispersion element for acousto-optic tunable filter deflected optical signal described in dispersion is concave reflection grating, described target detection fluorescence can be converted to directional light output, through described concave reflection grating dispersion and convert to after directional light, through described the first positive light coke lens focus in described detector.
Preferably, by described acousto-optic tunable filter deflected optical signal, through described the second collimation camera lens, convert directional light to, and the described dispersion element that converts directional light to through described the second collimation camera lens for dispersion is plane reflection grating or plane transmission grating; Described target detection fluorescence converts directional light through described dispersion element dispersion to by described the first collimation camera lens and passes through through described the first positive light coke lens focus in described detector again.
Beneficial effect of the present invention:
Adopt technical solution of the present invention, utilize acousto-optic tunable filter and dispersion element to realize instant configuration and the light splitting of microparticle fluorescence detection wavelength.
(1) realize in microparticle fluorescence testing process, according to kind of dyes with need dynamic-configuration target wavelength of fluorescence to be measured passage, can be by changing one or more centre frequency and band width and radio-frequency powers that act on the radio-frequency (RF) driving signal of acousto-optic tunable filter, any Selection Center wavelength, control fluorescence channel bandwidth, modulation fluorescence escape efficiency in the working range of Acousto-optical Tunable Filters.Having made up the microparticle fluorescence such as existing flow cytometer detects in beam-splitting structure, fluoroscopic examination divides optical channel to be comprised of the filter plate system of a plurality of dead center wavelength and bandwidth, during change fluorescence detection parameter, need to configure beam-splitting structure, the complex operations of displacement filter plate system, is difficult to the instant deficiency of adjusting.
(2), because acousto-optic tunable filter can be inputted a plurality of radio-frequency (RF) driving signal simultaneously, broken color throwaway unit has realized to reduce the mode of number of devices and has realized hyperchannel fluorescence by the ability of wavelength light splitting.Overcome because fluorescence parameter change multisystem beam-splitting structure is more complicated the defect that cost is higher.
(3) transmitance of acousto-optic tunable filter reaches more than 80%, can reach the application level with the consistent effect of many filter set cascade fluorescence passage.
(4) this system can be unique or with the combination of optical filter light-splitting method as the light splitting scheme for microparticle fluorescence detection.
Accompanying drawing explanation
Fig. 1 is basic block diagram of the present invention;
Fig. 2 is the situation schematic diagram that basic structure of the present invention is inputted in many radiofrequency signals simultaneously;
Fig. 3 is the schematic diagram that basic structure of the present invention increases the distressed structure one behind dispersion unit;
Fig. 4 is the schematic diagram that distressed structure one of the present invention increases the distressed structure two after phosphor collection camera lens;
Fig. 5 is the schematic diagram that distressed structure two of the present invention increases the distressed structure three after positive light coke mirror;
Fig. 6 is the schematic diagram that distressed structure three of the present invention increases the distressed structure four of collimation camera lens;
Fig. 7 is the schematic diagram that distressed structure two of the present invention increases the distressed structure five of positive light coke camera lens;
Fig. 8 is the schematic diagram that distressed structure five of the present invention increases the distressed structure six of collimation camera lens.
Number in the figure explanation: 1100, fluid forming unit, 1101, sheath fluid, 1102, sample, 1103, particulate, 1104, detection of particulates district, 1200, light is collected a minute photo detecting unit, 1201, acousto-optic tunable filter, 1202, radio driver, 1203, dispersion element, 1204, phosphor collection camera lens, 1205, the first positive light coke camera lens, 1206, detector, 1207, the first collimation camera lens, 1208, the second positive light coke camera lens, 1210, the second collimation camera lens, 1300, fluorescence excitation unit, 1301, excitation source, 1302, optical shaping unit.
Embodiment
Below with reference to the accompanying drawings and in conjunction with the embodiments, describe the present invention in detail.
Shown in Fig. 1, a kind of microparticle fluorescence detects wavelength and immediately configures beam splitting system, comprise excitation source 1301, optical shaping unit 1302 and acousto-optic tunable filter 1201, described excitation source 1301 is provided with optical shaping unit 1302 above, described optical shaping unit 1302 is provided with fluid forming unit 1100 above, described fluid forming unit 1100 is provided with acousto-optic tunable filter 1201 above, described acousto-optic tunable filter 1201 is connected with radio driver 1202, described fluid forming unit 1100 comprises sheath fluid 1101, sample 1102, particulate 1103 and detection of particulates district 1104.
Further, described acousto-optic tunable filter 1201 is provided with dispersion element 1203 above, described dispersion element 1203 be provided with detector 1206 below.
Further, between described fluid forming unit 1100 and described acousto-optic tunable filter 1201, be provided with phosphor collection camera lens 1204.
Further, described detector 1206 is provided with the first positive light coke camera lens 1205 above.
Further, between described phosphor collection camera lens 1204 and described acousto-optic tunable filter 1201, be provided with the second positive light coke camera lens 1208.
Further, between described the first positive light coke camera lens 1205 and described dispersion element 1203, be provided with the first collimation camera lens 1207.
Further, between described acousto-optic tunable filter 1201 and described dispersion element 1203, be provided with the second collimation camera lens 1210.
Further, described acousto-optic tunable filter 1201 is as core parts, and the wavelength coverage that described acousto-optic tunable filter 1201 covers and configuration quantity and IO interface are that the free type of air transmission and the optical-fiber type of Optical Fiber Transmission can be selected according to target wavelength characteristic.
Preferably, described radio driver 1202 possesses and changes radio-frequency (RF) driving signal frequency and realize the ability that fluorescence parameter passage dynamically changes centre wavelength and bandwidth; The radio-frequency (RF) driving signal of described radio driver 1202 can be single center frequency signal, can be also the stack of a plurality of independently different center frequency signals, and described center frequency value depends on the centre wavelength of target detection fluorescence; Described radio-frequency (RF) driving signal is the signal with certain bandwidth being worth centered by centre frequency.
Further, described dispersion element 1203 dispersions, by the target fluorescence of described acousto-optic tunable filter 1201 deflection outputs, can adopt grating or prism, and described grating can be reflection grating or transmission grating.
Further, described phosphor collection camera lens 1204 be high-NA camera lens and typical N A value should be greater than 0.6, described phosphor collection camera lens 1204 possesses collimation property, and the fluorescence excitation of tested particle is converted into approximate directional light, and the dispersion angle of described approximate directional light should be less than 0.5 degree.
Principle of the present invention:
The acousto-optic tunable filter 1201 of the bright middle use of this law is a kind of more novel acousto-optic light-splitting device, its inner acousto-optic crsytal is forming periodic index of refraction variation by radio-frequency (RF) driving signal under the ul-trasonic irradiation of transducer conversion, this periodic index of refraction variation has played the effect of similar grating, and the monochromatic plane wave deflection from non-deflection that meets as seen diffraction conditions is separated.Change radio-frequency (RF) driving signal frequency and will change ultrasonic frequency, change the cycle of periodic index of refraction variation, changed the wavelength of the monochromatic plane waves of acousto-optic tunable filter 1201 deflections simultaneously.Herein, what need to indicate is that so-called monochromatic plane wave remains the light wave that comprises certain wavelength width, and its centre wavelength is relevant to acousto-optic tunable filter radio-frequency (RF) driving signal centre frequency, and half band-width is relevant to radio-frequency (RF) driving signal centre frequency width.Current acousto-optic tunable filter 1201 has possessed the ability that can simultaneously input a plurality of independent radio-frequency (RF) driving signal, and the centre frequency that independently refers to a plurality of radio-frequency (RF) driving signal herein and band width and radio-frequency power are separate.
Embodiment:
Fluidic cell detection system is processed the particle to be measured of the target in tested sample by streaming focusing technology, realization is that single laminar flow flows through during by excitation-detection district, and this technology has kept particle in detection zone internal state, stablize, be excited evenly and can launch stable dye fluorescence scattering exciting light.Be scattered the size of exciting light relevant with size and the inner structure of target particle to be measured.Wavelength of fluorescence is relevant with concentration to the light activated fluorescent dye kind that is stimulated with intensity.Fluidic cell detection system is by measuring scattered light and dye fluorescence kind and intensity, and treated reality is the reporting objectives particle parameter features to be measured such as single-point figure, histogram, contour map.Fluidic cell detection system mainly comprises that fluid forming unit 1100, fluorescence excitation unit 1300, light are collected the hardware such as minute photo detecting unit 1200 and computer data process software forms.As shown in Figure 1:
Fluorescence excitation unit 1300 is comprised of excitation source 1301 and optical shaping unit 1302 conventionally.Excitation source preferably adopts the 488nmCUBA laser instrument of relevant company.The preferred protruding cylindrical lens of two different focal of quadrature each other in optical shaping unit.Exciting light is radiated at and on fluid, forms detection of particulates district 1104 after the shaping of optical shaping unit.
Fluid forming unit 1100 comprises sheath fluid 1101 and sample 1102, sample 1102 is under the effect of sheath fluid 1101, with streaming focus state, by detection of particulates district 1104, during by detection of particulates district 1104, can guarantee that target detection particulate 1103 is single placed in the middle by detection of particulates district 1104.Wherein the typical sizes of target detection particulate 1103 is 1-20 micron.When the sample 1102 that comprises target detection particulate 1103 under the parcel of sheath fluid 1101 when the detection of particulates district 1104, the generation fluorescence that will be excited of the fluorescent dye on it.Particulate 1103 scattering exciting lights and excited fluorescence general designation flashlight.Flashlight will be transmitted to the crystal window of acousto-optic tunable filter 1201.The brand of acousto-optic tunable filter 1201 comprises AA, CTI, Brimrose etc.This example preferably adopts the AOTFnC-400.650 type acousto-optic tunable filter 1201 of French AA company and the MDS radio driver 1202 matching.The deflection situation of acousto-optic tunable filter 1201 flashlight when many radiofrequency signals of radio driver 1203 drive jointly wherein, as shown in Figure 2.
Particle Scattering exciting light and excited fluorescence are under the effect of acousto-optic tunable filter 1201, with the light wave that input radio frequency drives signal to match, deflection is separated with non-deflection, arrive dispersion element 1203.Dispersion element 1203 can be a kind of of the elements such as grating, prism.600 line reflection blazed gratings of the preferred EO optics of this example, dispersion element 1203 is separated into monochromatic light by incident light.These ray spaces are relatively independent is incident to detector 1206.Detector 1206 is finally converted to the information such as the wavelength comprising in light signal, intensity, time the information of target detection particulate 1103 sizes, particulate dyestuff content for software analysis, as shown in Figure 3.
For the feature of this name is better described, preferred example also comprises the complicated of foregoing invention example, as shown in Figure 4:
Particle Scattering exciting light and excited fluorescence can be collected and collimate by high-NA phosphor collection camera lens 1204, preferably adopt 32340 lens of EO optics, its numerical aperture can reach this camera lens of 0.6. collection light is converted in directional light input acousto-optic tunable filter 1201, light is under the effect of acousto-optic tunable filter 1201, the light matching with input radio frequency driving signal, by deflection separated with non-deflection, arrives dispersion element 1203.It is relatively independent and be incident to detector 1206 that dispersion element 1203 is separated into monochromatic light space of lines by incident light.
For the feature of this name is better described, preferred example also comprises the complicated of foregoing invention example, as shown in Figure 5:
Particle Scattering exciting light and excited fluorescence can be collected and collimate by high-NA phosphor collection camera lens 1204, preferably adopt 32340 lens of EO optics, its numerical aperture can reach this camera lens of 0.6. collection light is converted in directional light input acousto-optic tunable filter 1201, light is under the effect of acousto-optic tunable filter 1201, the light matching with input radio frequency driving signal, by deflection separated with non-deflection, arrives dispersion element 1203.Dispersion element 1203 is separated into incident light monochromatic light and focuses on detector 1206 through the first positive light coke camera lens 1205.
For the feature of this name is better described, preferred example also comprises the complicated of foregoing invention example, as shown in Figure 6:
Particle Scattering exciting light and excited fluorescence can be collected and collimate by high-NA phosphor collection camera lens 1204, preferably adopt 32340 lens of EO optics, its numerical aperture can reach this camera lens of 0.6. collection light is converted in directional light input acousto-optic tunable filter 1201, light is under the effect of acousto-optic tunable filter 1201, the light matching with input radio frequency driving signal, by deflection separated with non-deflection, arrives dispersion element 1203.Dispersion element 1203 is separated into incident light monochromatic light and is converted to directional light output through the first collimation camera lens 1207, and each monochromatic light focuses on detector 1206 through the first positive light coke camera lens 1205.
For the feature of this name is better described, preferred example also comprises the complicated of foregoing invention example, as shown in Figure 7:
Particle Scattering exciting light and excited fluorescence can be collected and collimate by high-NA phosphor collection camera lens 1204, preferably adopt 32340 lens of EO optics, and its numerical aperture can reach this camera lens of 0.6. collection light is converted to directional light.Then through the second positive light coke camera lens 1208, focus in acousto-optic tunable filter 1201, its effect is can reduce the acousto-optic interaction causing due to the hot spot sound transit time to postpone.Light is under the effect of acousto-optic tunable filter 1201, and the light matching with input radio frequency driving signal, by deflection separated with non-deflection, arrives the dispersion element 1203 consisting of concave grating.Incident light is separated into monochromatic light with dispersion element 1203 and collimation is directional light output, and each monochromatic light focuses on detector 1206 through the first positive light coke camera lens 1205.
For the feature of this name is better described, preferred example also comprises the complicated of foregoing invention example, as shown in Figure 7:
Particle Scattering exciting light and excited fluorescence can be collected and collimate by high-NA phosphor collection camera lens 1204, preferably adopt 32340 lens of EO optics, and its numerical aperture can reach this camera lens of 0.6. collection light is converted to directional light.Then through the second positive light coke camera lens 1208, focus in acousto-optic tunable filter 1201, its effect is can reduce the acousto-optic interaction causing due to the hot spot sound transit time to postpone.Light is under the effect of acousto-optic tunable filter 1201, and the light matching with input radio frequency driving signal, by deflection separated with non-deflection, is converted to directional light through the second collimation camera lens 1210 and arrives the dispersion element 1203 that plane grating forms.Dispersion element 1203 is separated into incident light monochromatic light and is converted to directional light output through the first collimation camera lens 1207, and each monochromatic light focuses on detector 1206 through the first positive light coke camera lens 1205.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (11)
1. a microparticle fluorescence detects wavelength and immediately configures beam splitting system, comprise excitation source (1301), optical shaping unit (1302) and acousto-optic tunable filter (1201), it is characterized in that, described excitation source (1301) is provided with described optical shaping unit (1302) above, described optical shaping unit (1302) is provided with fluid forming unit (1100) above, described fluid forming unit (1100) is provided with acousto-optic tunable filter (1201) above, described acousto-optic tunable filter (1201) is connected with radio driver (1202), described fluid method of forming unit (1100) comprises sheath fluid (1101), sample (1102), particulate (1103) and detection of particulates district (1104).
2. microparticle fluorescence according to claim 1 detects wavelength and immediately configures beam splitting system, it is characterized in that, described acousto-optic tunable filter (1201) is provided with dispersion element (1203) above, described dispersion element (1203) be provided with above detector (1206).
3. microparticle fluorescence according to claim 1 detects wavelength and immediately configures beam splitting system, it is characterized in that, between described fluid forming unit (1100) and described acousto-optic tunable filter (1201), is provided with phosphor collection camera lens (1204).
4. microparticle fluorescence according to claim 2 detects wavelength and immediately configures beam splitting system, it is characterized in that, described detector (1206) is provided with the first positive light coke camera lens (1205) above.
5. microparticle fluorescence according to claim 3 detects wavelength and immediately configures beam splitting system, it is characterized in that, is provided with the second positive light coke camera lens (1208) between described phosphor collection camera lens (1204) and described acousto-optic tunable filter (1201).
6. microparticle fluorescence according to claim 4 detects wavelength and immediately configures beam splitting system, it is characterized in that, between described the first positive light coke camera lens (1205) and described dispersion element (1203), is provided with the first collimation camera lens (1207).
7. microparticle fluorescence according to claim 1 detects wavelength and immediately configures beam splitting system, it is characterized in that, is provided with the second collimation camera lens (1210) between described acousto-optic tunable filter (1201) and described dispersion element (1203).
8. microparticle fluorescence according to claim 1 detects wavelength and immediately configures beam splitting system, it is characterized in that, described acousto-optic tunable filter (1201) is as core parts, and the wavelength coverage that described acousto-optic tunable filter (1201) covers and configuration quantity and IO interface are that the free type of air transmission or the optical-fiber type of Optical Fiber Transmission can be selected according to target wavelength characteristic.
9. microparticle fluorescence according to claim 1 detects wavelength and immediately configures beam splitting system, it is characterized in that, described radio driver (1202) possesses the radio-frequency (RF) driving signal of change frequency and realizes the ability that fluorescence parameter passage dynamically changes centre wavelength and bandwidth; The radio-frequency (RF) driving signal of described radio driver (1202) can be single center frequency signal, can be also the stack of a plurality of independently different center frequency signals, and described center frequency value depends on the centre wavelength of target detection fluorescence; Described radio-frequency (RF) driving signal is the signal with certain bandwidth being worth centered by centre frequency.
10. microparticle fluorescence according to claim 2 detects wavelength and immediately configures beam splitting system, it is characterized in that, described dispersion element (1203) dispersion is by the target fluorescence of described acousto-optic tunable filter (1201) deflection output, can adopt grating or prism, described grating is reflection grating or transmission grating.
11. microparticle fluorescence according to claim 3 detect wavelength and immediately configure beam splitting system, it is characterized in that, described phosphor collection camera lens (1204) is that high-NA camera lens and typical N A value should be greater than 0.6, described phosphor collection camera lens (1204) possesses collimation property, the fluorescence excitation of tested particle is converted into approximate directional light, and the dispersion angle of described approximate directional light should be less than 0.5 degree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310478988.8A CN103575712A (en) | 2013-10-15 | 2013-10-15 | Particle fluorescence detection wavelength instant configuration beam splitting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310478988.8A CN103575712A (en) | 2013-10-15 | 2013-10-15 | Particle fluorescence detection wavelength instant configuration beam splitting system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103575712A true CN103575712A (en) | 2014-02-12 |
Family
ID=50047951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310478988.8A Pending CN103575712A (en) | 2013-10-15 | 2013-10-15 | Particle fluorescence detection wavelength instant configuration beam splitting system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103575712A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483254A (en) * | 2014-12-29 | 2015-04-01 | 中国科学院长春光学精密机械与物理研究所 | Multi-color multi-parameter portable flow cytometer |
| CN106769704A (en) * | 2015-11-20 | 2017-05-31 | 北京汇丰隆经济技术开发有限公司 | A kind of bioaerosol particle optics detection means |
| CN107515051A (en) * | 2017-07-20 | 2017-12-26 | 武汉科技大学 | Wavelength measurement method and system based on acoustooptical effect |
| CN110057798A (en) * | 2019-04-26 | 2019-07-26 | 江苏师范大学 | A kind of streaming sample multi-wavelength fluorescence detection method |
| CN110093271A (en) * | 2019-06-06 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Cell sorting device |
| CN113092428A (en) * | 2021-04-02 | 2021-07-09 | 安图实验仪器(郑州)有限公司 | Multiple fluorescence detection method, device, equipment and system |
| WO2023102675A1 (en) * | 2021-12-06 | 2023-06-15 | Shenzhen Genorivision Technology Co., Ltd. | Flow cytometry systems with image sensors |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6210973B1 (en) * | 1994-12-05 | 2001-04-03 | John W. Pettit | Method for automated flourescent tagged sample detecting |
| US20040251436A1 (en) * | 2003-06-12 | 2004-12-16 | Philippe Goix | Single detector multicolor particle analyzer and method |
| CN103048258A (en) * | 2012-12-26 | 2013-04-17 | 江西科技师范大学 | Light splitting device for flow cytometer |
| CN203534970U (en) * | 2013-10-15 | 2014-04-09 | 中国科学院苏州生物医学工程技术研究所 | Instant configuring and beam-splitting system for particle fluorescence detection wavelengths |
-
2013
- 2013-10-15 CN CN201310478988.8A patent/CN103575712A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6210973B1 (en) * | 1994-12-05 | 2001-04-03 | John W. Pettit | Method for automated flourescent tagged sample detecting |
| US20040251436A1 (en) * | 2003-06-12 | 2004-12-16 | Philippe Goix | Single detector multicolor particle analyzer and method |
| CN103048258A (en) * | 2012-12-26 | 2013-04-17 | 江西科技师范大学 | Light splitting device for flow cytometer |
| CN203534970U (en) * | 2013-10-15 | 2014-04-09 | 中国科学院苏州生物医学工程技术研究所 | Instant configuring and beam-splitting system for particle fluorescence detection wavelengths |
Non-Patent Citations (1)
| Title |
|---|
| 王玉田等: "一种新型农药浓度荧光测量系统的研究", 《测试技术学报》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483254A (en) * | 2014-12-29 | 2015-04-01 | 中国科学院长春光学精密机械与物理研究所 | Multi-color multi-parameter portable flow cytometer |
| CN106769704A (en) * | 2015-11-20 | 2017-05-31 | 北京汇丰隆经济技术开发有限公司 | A kind of bioaerosol particle optics detection means |
| CN107515051A (en) * | 2017-07-20 | 2017-12-26 | 武汉科技大学 | Wavelength measurement method and system based on acoustooptical effect |
| CN107515051B (en) * | 2017-07-20 | 2020-11-03 | 武汉科技大学 | Wavelength measuring method and system based on acousto-optic effect |
| CN110057798A (en) * | 2019-04-26 | 2019-07-26 | 江苏师范大学 | A kind of streaming sample multi-wavelength fluorescence detection method |
| CN110093271A (en) * | 2019-06-06 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Cell sorting device |
| CN110093271B (en) * | 2019-06-06 | 2024-06-11 | 中国科学院苏州生物医学工程技术研究所 | Cell sorting device |
| CN113092428A (en) * | 2021-04-02 | 2021-07-09 | 安图实验仪器(郑州)有限公司 | Multiple fluorescence detection method, device, equipment and system |
| WO2023102675A1 (en) * | 2021-12-06 | 2023-06-15 | Shenzhen Genorivision Technology Co., Ltd. | Flow cytometry systems with image sensors |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103575712A (en) | Particle fluorescence detection wavelength instant configuration beam splitting system | |
| CN111344551B (en) | Optical configuration method of spectrum scattering flow cytometer | |
| CN104949958B (en) | Novel Raman probe based on optical fiber beam splitter | |
| US20090059207A1 (en) | Method and device for measuring photoluminescence, absorption and diffraction of microscopic objects in a fluid | |
| US20220082488A1 (en) | Methods of forming multi-color fluorescence-based flow cytometry panel | |
| WO2018052798A1 (en) | Flow cytometer with optical equalization | |
| JP6019039B2 (en) | Electro-optic flow measuring device | |
| CN106841014B (en) | Flow cytometer collecting lens and optical system of dual-color laser flow cytometer | |
| CN103852409B (en) | The imaging system of hemocyte in flow cytometer | |
| JP2018527559A (en) | System and method for adjusting cytometer measurements | |
| CN203534970U (en) | Instant configuring and beam-splitting system for particle fluorescence detection wavelengths | |
| CN105510297A (en) | Raman fluorescence spectrum testing system and optical signal collector thereof | |
| CN106680186B (en) | A kind of flow cytometer polymorphic type scattering optical detection system | |
| CN106918544A (en) | A kind of flow cytometer showed instrument system based on fluorescence excitation light source | |
| CN104280327B (en) | A kind of streaming phosphor collection optical system | |
| US20240027457A1 (en) | High parameter reagent panel and reagent kit for effective detection of aberrant cells in acute myeloid leukemia | |
| CN103245643A (en) | Optical system for fluorescence detection and fine particle analyzing apparatus | |
| Lin et al. | High-throughput fluorescence detections in microfluidic systems | |
| CN115096795A (en) | Flow type fluorescence detection optical system | |
| CN209280527U (en) | A kind of particle analyzer and its optically detecting module | |
| CN108982431B (en) | On-line fluorescence detection device | |
| CN113899677A (en) | Reflective light splitting module and light splitting method for flow cytometer detection | |
| EP4100717A1 (en) | Apparatus and method for cyclic flow cytometry using particularized cell identification | |
| CN110530783B (en) | Lateral beam collection method and device for flow cytometer and flow cytometer | |
| CN108414480B (en) | Crude oil fluorescence measuring device and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140212 |