CN110093271A - Cell sorting device - Google Patents
Cell sorting device Download PDFInfo
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- CN110093271A CN110093271A CN201910493156.0A CN201910493156A CN110093271A CN 110093271 A CN110093271 A CN 110093271A CN 201910493156 A CN201910493156 A CN 201910493156A CN 110093271 A CN110093271 A CN 110093271A
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- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000002699 waste material Substances 0.000 claims abstract description 9
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention discloses a kind of cell sorting devices, it includes sprue, injection port, waste liquid port and collection port with flow passage structure, flow passage structure, and sprue downstream is divided into three branches, middle branch connects the waste liquid port, and the symmetrical branch of the two of both sides connects the collection port；Streamwise is disposed with focal zone, detection zone and screening area on sprue；Focusing of the focal zone for realizing the particle in the sprue in three dimensions, detection zone for detecting target particles, screening area for realizing target particles and non-targeted particle sorting.The present invention is focused cell using sound wave, saves sheath fluid, sample flux is promoted.Flow velocity is slowed down, and the promotion of subsequent detection sensitivity is conducive to；Cell sorting device of the invention is not damaged to cell, can be used for conventional fluidic cell sorting, it can also be used to which the screening of rare cell is of great significance to the noninvasive Prenatal Screening of promotion, tumor prognosis detection.
Description
Technical field
The present invention relates to biologic grain detection and manipulation technical field, in particular to a kind of cell sorting devices.
Background technique
Detection captures rare cell very little in blood, and the state of an illness of the early diagnosis and patient that facilitate disease is supervised
It surveys.Existing flow cell sorter is there are bulky, structure is complicated, the problem of when need to clean pipeline, labour cost repeatedly；And its
Assorting room is completed in air, open system, can generate the Aerosol Pollution comprising samples such as cell, bacterium, viruses, limitation
Its clinical application.The cell sorting system of the companies such as BD, Beckman Coulter uses Jet-in-Air's mostly at present
Electrostatic deflection separate mode (United States Patent (USP) No. 3710933, No. 3826364), although can be with high-speed separation cell, due to it
Higher hydrodynamic shear can generate damage to cell, influence its activity and gene expression.Such as in regenerative cell's treatment, stem cell
In research, there is a problem of that survival rate is low using the cell that conventional electrostatic cell sorter sorts.Simultaneously in cytothesis, turn base
In the sample research crossed by sample or virus/bacterium infection, it is ensured that the closed and aseptic of environment is very crucial problem.
For rare cell sorting, high flux screening is important.Existing flow sorter due to using sheath fluid focus,
There is limitation on sample introduction flux, content is low in the sample for generally below 100 μ L/min. rare cells, it usually needs biggish
Sample size is to be enriched with, therefore progress high efficiency extraction makes the promotion of sample flow particularly important within the cell survival time.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is that providing a kind of cell sorting
Device.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: a kind of cell sorting device, with runner
Structure, the flow passage structure include sprue, injection port, waste liquid port and collection port, and the sprue downstream is divided into three branches,
Middle branch connects the waste liquid port, and the symmetrical branch of the two of both sides connects the collection port；Streamwise on the sprue
It is disposed with focal zone, detection zone and screening area；
Focusing of the focal zone for realizing the particle in the sprue in three dimensions, the detection zone are used for
Detect target particles, the screening area for realizing target particles and non-targeted particle sorting.
Preferably, at least one the PZT (piezoelectric transducer) list being bonded on the sprue is provided on the focal zone
Member, on the center line for the particle in the three-dimensional space in the sprue to be focused on to the sprue or the mainstream
On the straight line of the approximate centerline in road, make particle successively single arrangement flowing in the sprue；On the screening area
A Piezoelectric transducer elements are provided with, two standing wave nodel lines are formed in the sprue width range, for making target
Numerical density is flowed into two symmetrical branches of the width direction two sides of the sprue.
Preferably, 2 Piezoelectric transducer elements being bonded on the sprue are provided on the focal zone: first
Piezoelectric transducer elements and the second Piezoelectric transducer elements, the Piezoelectric transducer elements of the screening area setting are that third piezoelectricity changes
It can device unit；
Using the sprue flow direction as X-axis, the width direction of the sprue be Y-axis, the depth of the sprue
Direction is Z axis, and first Piezoelectric transducer elements and the second Piezoelectric transducer elements are for realizing particle in the sprue
X/Y plane and XZ plane in focusing；The X/Y plane of the sprue is arranged in first Piezoelectric transducer elements or XZ is put down
In face, second Piezoelectric transducer elements are arranged in the X/Y plane or XZ plane of the sprue, and the third piezoelectricity changes
Energy device unit is arranged in the X/Y plane or XZ plane of the sprue；Wherein, each Piezoelectric transducer elements include that setting exists
Single PZT (piezoelectric transducer) on the mainstream pipeline outer wall, or opposite width or the depth direction two sides for being bonded in the sprue
Outer wall on 2 PZT (piezoelectric transducer)s.
Preferably, first Piezoelectric transducer elements and the second Piezoelectric transducer elements are arranged in mutually in 90 ° two
In a plane, first Piezoelectric transducer elements are arranged in the X/Y plane of the sprue, first PZT (piezoelectric transducer)
The wavelength for the ultrasonic standing wave that unit generates is 2 times of the sprue width of Y-direction, forms one in sprue width range and stays
Nodal line, nodel line position are the middle position of main width of flow path, are the sound wave of continuous action, put down for particle to be focused on XY
Face；Second Piezoelectric transducer elements are arranged in the XZ plane of the sprue, and second Piezoelectric transducer elements produce
The wavelength of raw ultrasonic standing wave is 2 times of Z-direction sprue depth, and a standing wave nodel line is formed in sprue depth bounds,
Nodel line position is the middle position of main flow channel depth, is the sound wave of continuous action, for focusing on particle in XZ plane.
Preferably, the wavelength for the ultrasonic standing wave that the third Piezoelectric transducer elements generate is equal to the sprue side Y
To width, two standing wave sections symmetrical along the center line of the sprue are formed in the sprue width range
Line is the sound wave of impulse action, enters the sprue for flowing target particles stress along two standing wave nodel lines
In the symmetrical branch of the two of width direction two sides.
Preferably, first Piezoelectric transducer elements and the second Piezoelectric transducer elements are arranged at X/Y plane or XZ
In plane, one of them focusing for realizing particle in X/Y plane, another is poly- in XZ plane for realizing particle
It is burnt.
Preferably, when the width of the sprue is equal to its depth, first piezoelectricity is saved on the focal zone and is changed
Can device unit or the second Piezoelectric transducer elements, only by a Piezoelectric transducer elements, at the same realize particle in X/Y plane and
Focusing in XZ plane.
Preferably, described two symmetrical branches connect the collection port after end merges jointly.
Preferably, described two symmetrical branches are connected respectively to two collection ports in end nonjoinder.
Preferably, the fluid in the sprue remains laminar condition.
The present invention is include at least the following beneficial effects:
1, whether the present invention judges target using laser detection, targeting screening is carried out in downstream, even if cell is physical
Matter is similar, also can use immunofluorescence characteristic and is effectively distinguished；
2, the present invention pushes the target cell in fluid to deviate original path in screening area using piezoelectric element excitation sound wave,
Cell sorting can be fast implemented, simultaneously because mechanical force in cell, does not influence its activity；
3, the present invention is focused cell using sound wave, saves sheath fluid, sample flux is promoted.Flow velocity is subtracted
Slowly, be conducive to the promotion of subsequent detection sensitivity；
4, flow passage structure of the present invention is simple, and head piece quantity is few, is conducive to exhaust bubble and maintains the stabilization of fluid environment；
5, the present invention can avoid runner dead angle in such a way that head piece is drawn in side, reduce residual, reduce the friendship between sample
Fork pollution；
6, fluid channel of the present invention is bonded by two layers of the glass containing microtubule, plastics, metal or polymer material, is viscous
It connects, overall structure sterile sealing, to there are the samples of biohazard can also be applicable in, plug and play can after disposable
Abandon sorting chip；
7, cell sorting device of the invention is not damaged to cell, can be used for conventional fluidic cell sorting, it can also be used to
The screening of rare cell is of great significance to the noninvasive Prenatal Screening of promotion, tumor prognosis detection.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the cell sorting device in the embodiment of the present invention 1；
Fig. 2 is the structural schematic diagram of the cell sorting device in the embodiment of the present invention 2；
Fig. 3 is the structural schematic diagram of the cell sorting device in the embodiment of the present invention 3；
Fig. 4 is the structural schematic diagram of the cell sorting device in the embodiment of the present invention 4；
Fig. 5 is the functional block diagram of detection module of the invention.
Description of symbols:
1- sprue；2- injection port；3- waste liquid port；4- collection port；The focal zone 5-；6- detection zone；The screening area 7-；8- first
Piezoelectric transducer elements；9-the second Piezoelectric transducer elements；10-third Piezoelectric transducer elements；11-laser detection points；
12-target particles；13-non-targeted particles.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
As shown in Figs. 1-5, a kind of cell sorting device of the present embodiment, with flow passage structure, flow passage structure includes master
Runner 1, injection port 2, waste liquid port 3 and collection port 4,1 downstream of sprue are divided into three branches, and middle branch connects waste liquid port 3, and two
The symmetrical branch of the two of side connects collection port 4；Streamwise is disposed with focal zone 5, detection zone 6 and screening on sprue 1
Area 7；
Focusing of the focal zone 5 for realizing the particle in sprue 1 in three dimensions, detection zone 6 is for detecting target
Particle, screening area 7 for realizing target particles 12 and non-targeted particle 13 sorting.Detection zone 6 uses laser detection, judges to locate
Whether particle at laser detection point 11 is target particles, and the Piezoelectric transducer elements of screening area 7 are according to the judgement of detection zone 6
As a result it is acted.The present invention carries out high-throughput grain sorting using ultrasound, and targeting is realized to the detection of particle by laser
Screening.
In the present invention, cell sorting device can be a sorting chip, and flow passage structure is microchannel, is formed in sorting chip
Portion.Flow passage structure can be formed by substrate and cover plate, and cover plate bottom offers plumbing chase, and cover plate sealing is attached on substrate,
Make to form flow passage structure in the plumbing chase between cover plate and piezoelectric substrate.Substrate and cover plate can for glass, plastics, metal or
The materials such as polymer.Overall structure sterile sealing, to there are the samples of biohazard can also be applicable in, plug and play disposably makes
With rear discardable sorting unit.In another embodiment, the sprue 1 in flow passage structure stretches to be formed through material, then with
Subsequent lateral is connected.In a preferred embodiment, the width of each pipeline is 10~500 μm, height in flow passage structure
Degree is 20~200 μm.Fluid in flow passage structure remains laminar condition.
Sample cell enters before cell sorting device the fluorescent staining processing for first carrying out target cell.Target cell is carried out
Dyeing (by the specific antibody that can be specifically bound with target cell that fluorescent marker is added), utilizes streaming fluorescence detection skill
Art, by the detection of fluorescent dyes at the laser detection point pairs of specific antibody in conjunction with target cell, to identify target
Cell, to carry out target cell extraction.
Detection zone 6 can specifically be achieved by the following scheme detection: the detection module on detection zone 6 includes hot spot excitation modulation
System, light signal detection system, data collection and analysis and control module, piezoelectric driving module；Hot spot excites modulating system to generate
Laser simultaneously forms ellipse light spot, is radiated at the fixation position on 6 sprue 1 of detection zone, and irradiation excitation flows through each of detection zone 6
Cell generates fluorescence and scattering light；Light signal detection system is collected and detects to the fluorescence and scattering light of generation, forms light
Signal message, then data collection and analysis and control module are sent to after obtained optical signal information is converted to electric signal.Data
Received electric signal is carried out quantization for collection analysis and control module and analysis is handled, and by processing result and sieve set by user
It selects condition to carry out logic judgment, the pressure for screening trigger signal to screening area 7 is sent if meeting screening conditions set by user
Electric drive module；Piezoelectric driving module will screen trigger signal and be converted into driving the third Piezoelectric transducer elements in screening area 7
High voltage signal, so that it is generated the motion path that sound field changes target cell, thus by target cell and untargeted cells point
It is exported to different sortings, realizes cell sorting.
It is provided at least one Piezoelectric transducer elements being bonded on sprue 1 on focal zone 5, is used for sprue 1
Particle in interior three-dimensional space focuses on the center line of sprue 1 or the straight line of the approximate centerline of sprue 1
On, make particle successively single arrangement flowing in sprue 1；A Piezoelectric transducer elements are provided on screening area 7, in master
Two standing wave nodel lines are formed in 1 width range of runner, for making target particles stress be flowed into the width direction two sides of sprue 1
Two symmetrical branches in.
Since screening area 7 is located at 6 downstream of detection zone, which need to postpone to produce after waiting certain cell flowing time
Raw, when cell moves to 7 position of downstream screening area by detection zone 6, Piezoelectric Ceramic module generates certain pulsewidth and certain
The driving signal of voltage, the signal control sound field and generate, and then push target cell, realize the difference of the optical signalling according to detection
Purpose different, that cell is separated.Wherein, the amplitude of driving voltage and the deformation of piezoelectric ceramics are positively correlated, and voltage is higher, pushes away
Power is bigger.
When screening area 7 determines target cell, using key player on a team's method or it is counter select method, when using key player on a team's method, with
Meet the signal of target cell characteristic as judgment basis, control third PZT (piezoelectric transducer) generation movement, namely to detect
Positive value is used as gating foundation, the gating in the positive value region detected, when detection signal is fallen into door, third piezoelectric energy-conversion
Device generation movement, makes target particles stress be flowed into collection port 4.Using it is counter select method when, to meet the letter of untargeted cells characteristic
Number it is used as judgment basis, control third PZT (piezoelectric transducer) generation movement is being examined that is, using the positive value that detects as gating foundation
The outer gating in positive value region measured, when detecting signal and falling into door, third PZT (piezoelectric transducer) generation is acted, make target particles by
Power is flowed into collection port 4.It, can be with CD71 when using key player on a team's method for screening the fetal nucleated red blood in maternal blood
The positive signal of (fetal nucleated red blood specific antibody) is that foundation judges the arrival of target cell, to control piezoelectric energy-conversion
The movement of device.Using it is counter select method when, can with the negative signal of CD45 (leukocyte specific antibody) be according to determine target cell
Arrival, to control the movement of PZT (piezoelectric transducer).When the antibody specificity of target cell is bad, method is selected to can be improved using counter
Capture radio.
2 Piezoelectric transducer elements being bonded on sprue 1: the first Piezoelectric transducer elements 8 are provided on focal zone 5
With the second Piezoelectric transducer elements 9, the Piezoelectric transducer elements that screening area 7 is arranged are third Piezoelectric transducer elements；
Using 1 flow direction of sprue as X-axis, the width direction of sprue 1 be Y-axis, the depth direction of sprue 1 is Z axis,
First Piezoelectric transducer elements 8 and the second Piezoelectric transducer elements 9 for realizing particle sprue 1 X/Y plane and XZ plane
Interior focusing；First Piezoelectric transducer elements 8 are arranged in the X/Y plane or XZ plane of sprue 1, the second PZT (piezoelectric transducer) list
Member 9 is arranged in the X/Y plane or XZ plane of sprue 1, third Piezoelectric transducer elements be arranged in sprue 1 X/Y plane or
In XZ plane；Wherein, each Piezoelectric transducer elements include the single PZT (piezoelectric transducer) being arranged on the sprue side wall, or
It is opposite 2 PZT (piezoelectric transducer) (i.e. piezoelectric energy-conversions being bonded on the width of the sprue or the outer wall of depth direction two sides
Device group).First Piezoelectric transducer elements 8 and 9 position of the second Piezoelectric transducer elements are interchangeable, may be provided at identical plane,
It may be provided at different planes, it is only necessary to which focusing of the particle in X/Y plane and XZ plane is able to achieve i.e. by the cooperation of the two
It can.
The wavelength for the ultrasonic standing wave that third Piezoelectric transducer elements generate is equal to the direction (or being approximately equal to) sprue 1Y
Width forms the two standing wave nodel lines symmetrical along the center line of sprue 1 in 1 width range of sprue, makees for pulse
Sound wave enters sprue 1 along two standing wave nodel line flowings for making target particles by the effect of ultrasonic radiation force
Width direction two sides two symmetrical branches in, finally enter collection port 4.The action time of third Piezoelectric transducer elements compared with
It is short, target cell can be allowed to generate enough offsets relative to original flow path as Basis.
Wherein, in order to reduce the formation at flow path dead angle, runner side sample introduction can be used and go out the mode of sample (i.e. in runner side
Draw inlet and outlet), it is more advantageous to reduction detection sample cross contamination.
Wherein, the fluid in sprue 1 remains laminar condition.
Wherein, reference is schemed, and in the present embodiment, two symmetrical branches connect collection port 4 after end merges jointly.And another
In kind embodiment, two symmetrical branches are connected respectively to two collection ports 4 in end nonjoinder.It can be in entire fluid chip
Two collection ports 4 are drawn in side, and this also have the advantage that reduce to detect sample cross contamination.
Several specific embodiments presented below, with the invention will be further described.
Embodiment 1
Referring to Fig.1, in the present embodiment, the first Piezoelectric transducer elements 8 and the setting of the second Piezoelectric transducer elements 9 are being mutually
In 90 ° of two planes, the first Piezoelectric transducer elements 8 are arranged in the X/Y plane of sprue 1, and first group of PZT (piezoelectric transducer) produces
The wavelength of raw ultrasonic standing wave is 2 times (or about 2 times) of 1 width of sprue of Y-direction, the shape in 1 width range of sprue
At a standing wave nodel line, nodel line position is middle position (or the interposition close to 1 width of sprue of 1 width of sprue
Set), it is the sound wave of continuous action, for particle to be focused on X/Y plane, i.e. particle is in single-row movement in X/Y plane；Second pressure
Electric transducer unit 9 is arranged in the XZ plane of sprue 1, and the wavelength for the ultrasonic standing wave that second group of PZT (piezoelectric transducer) generates is Z
2 times (or about 2 times) of 1 depth of direction sprue form a standing wave nodel line, nodel line position in 1 depth bounds of sprue
It is the middle position (or close to the middle position of 1 width of sprue) of 1 depth of sprue, is the sound wave of continuous action, is used for
Particle is focused in XZ plane, i.e., particle is in single-row movement in XZ plane.And wherein, 8 He of the first Piezoelectric transducer elements
The position of second Piezoelectric transducer elements 9 is interchangeable.
Embodiment 2
Referring to Fig. 2, in the present embodiment, the first Piezoelectric transducer elements 8 and the second Piezoelectric transducer elements 9 are arranged at XY
In plane or XZ plane, wherein one group of focusing for realizing particle in X/Y plane, another group flat in XZ for realizing particle
Focusing in face.
Embodiment 3
When the width of sprue 1 is equal to its depth, referring to Fig. 3, in the present embodiment, a piezoelectricity is only set on focal zone 5
Transducer unit, while realizing focusing of the particle in X/Y plane and XZ plane.By the parameter to Piezoelectric transducer elements into
Row control, (vibration frequency is mainly related to the depth and width of sprue 1, sprue 1 for the vibration frequency of adjusting sprue wall
When width is equal to depth, width two sidewalls are identical as depth two sidewalls vibration frequency), so as to pass through a PZT (piezoelectric transducer)
Unit realizes focusing of the particle in X/Y plane and XZ plane.
Embodiment 4
Due to third group PZT (piezoelectric transducer) generate be a period of time standing wave, if within its action time and the zone of action
Exist simultaneously target and non-targeted particle, then it may there is a situation where non-targeted particles to be also pushed into collection conduit.For this purpose,
In the present embodiment, referring to Fig. 4, multiple unit cascaded modes can be used, the particle of collection is sorted again, to improve most
The purity of target particles is obtained eventually.When using cascade system, the collection port 4 of previous stage unit is the injection port 2 of next stage unit.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (10)
1. a kind of cell sorting device, which is characterized in that it includes sprue, sample introduction with flow passage structure, the flow passage structure
Mouthful, waste liquid port and collection port, the sprue downstream is divided into three branches, and middle branch connects the waste liquid port, the two of both sides
Symmetrical branch connects the collection port；Streamwise is disposed with focal zone, detection zone and screening area on the sprue；
Focusing of the focal zone for realizing the particle in the sprue in three dimensions, the detection zone is for detecting
Target particles, the screening area for realizing target particles and non-targeted particle sorting.
2. cell sorting device according to claim 1, which is characterized in that be provided on the focal zone described in being bonded in
At least one Piezoelectric transducer elements on sprue, for the particle in the three-dimensional space in the sprue to be focused on institute
It states on the center line of sprue or on the straight line of the approximate centerline of the sprue, makes particle in the sprue
Successively single arrangement flowing；A Piezoelectric transducer elements are provided on the screening area, in the sprue width range
Two standing wave nodel lines of interior formation, for make target particles stress be flowed into the sprue width direction two sides two symmetrical branch
Lu Zhong.
3. cell sorting device according to claim 2, which is characterized in that be provided on the focal zone described in being bonded in
2 Piezoelectric transducer elements on sprue: the first Piezoelectric transducer elements and the second Piezoelectric transducer elements, the screening area
The Piezoelectric transducer elements of setting are third Piezoelectric transducer elements；
Using the sprue flow direction as X-axis, the width direction of the sprue be Y-axis, the depth direction of the sprue
For Z axis, first Piezoelectric transducer elements and the second Piezoelectric transducer elements for realizing particle the sprue XY
Focusing in plane and XZ plane；The X/Y plane or XZ plane of the sprue is arranged in first Piezoelectric transducer elements
Interior, second Piezoelectric transducer elements are arranged in the X/Y plane or XZ plane of the sprue, the third piezoelectric energy-conversion
Device unit is arranged in the X/Y plane or XZ plane of the sprue；Wherein, each Piezoelectric transducer elements include being arranged in institute
The single PZT (piezoelectric transducer) on mainstream pipeline outer wall is stated, or with respect to the width or depth direction two sides for being bonded in the sprue
2 PZT (piezoelectric transducer)s on outer wall.
4. cell sorting device according to claim 3, which is characterized in that first Piezoelectric transducer elements and second
Piezoelectric transducer elements are arranged in two planes mutually in 90 °, and first Piezoelectric transducer elements are arranged in the mainstream
In the X/Y plane in road, the wavelength for the ultrasonic standing wave that first Piezoelectric transducer elements generate is the 2 of the sprue width of Y-direction
Times, a standing wave nodel line is formed in sprue width range, nodel line position is the middle position of main width of flow path, for lasting work
Sound wave, for particle to be focused on X/Y plane；The XZ that the sprue is arranged in second Piezoelectric transducer elements is flat
In face, the wavelength for the ultrasonic standing wave that second Piezoelectric transducer elements generate is 2 times of Z-direction sprue depth, in mainstream
A standing wave nodel line is formed in road depth bounds, it is the sound wave of continuous action that nodel line position, which is the middle position of main flow channel depth,
For particle to be focused in XZ plane.
5. cell sorting device according to claim 3, which is characterized in that the third Piezoelectric transducer elements generated
The wavelength of ultrasonic standing wave is equal to the width of the sprue Y-direction, is formed in the sprue width range along the mainstream
Two symmetrical standing wave nodel lines of the center line in road are the sound wave of impulse action, for staying target particles stress along two
Nodal line is flowed and is entered in two symmetrical branches of the width direction two sides of the sprue.
6. cell sorting device according to claim 3, which is characterized in that first Piezoelectric transducer elements and second
Piezoelectric transducer elements are arranged in X/Y plane or XZ plane, one of them focusing for realizing particle in X/Y plane,
Another focusing for realizing particle in XZ plane.
7. cell sorting device according to claim 3, which is characterized in that the width of the sprue is equal to its depth
When, first Piezoelectric transducer elements or the second Piezoelectric transducer elements are saved on the focal zone, only pass through a piezoelectricity
Transducer unit, while realizing focusing of the particle in X/Y plane and XZ plane.
8. cell sorting device according to any one of claims 1-7, which is characterized in that the two symmetrical branch exists
End connects the collection port after merging jointly.
9. cell sorting device according to any one of claims 1-7, which is characterized in that the two symmetrical branch exists
End nonjoinder is connected respectively to two collection ports.
10. cell sorting device according to any one of claims 1-7, which is characterized in that in the sprue
Fluid remains laminar condition.
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