CN207336317U - A kind of ultrasound flow cytometer - Google Patents
A kind of ultrasound flow cytometer Download PDFInfo
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- CN207336317U CN207336317U CN201721201088.9U CN201721201088U CN207336317U CN 207336317 U CN207336317 U CN 207336317U CN 201721201088 U CN201721201088 U CN 201721201088U CN 207336317 U CN207336317 U CN 207336317U
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Abstract
Technical field of biotechnology is the utility model is related to, discloses a kind of ultrasonic flow cytometer, including：Sample cell, for storing sample liquid；Sheath liquid pipe, for storing sheath fluid；Two the first branch pipes are extended in flow chamber, including the focal zone passage of connection and sorting area's passage, the one end of focal zone passage away from sorting area's passage, and the first branch pipe is connected with sample cell with sheath liquid pipe respectively；First ultrasonic laser transducer, is arranged on the outside of the passage of focal zone, for producing the first sound wave in the passage of focal zone；Second ultrasonic laser transducer, is arranged on the outside of sorting area's passage, for producing the second sound wave in sorting area's passage.The laser sent using ultrasonic laser transducer is focused cell, sorts, improve ultrasound source capacity usage ratio so as to produce focusing acoustic field, standing-wave sound field or pulsed sound.
Description
Technical field
It the utility model is related to technical field of biotechnology, and in particular to a kind of ultrasound flow cytometer.
Background technology
Flow cytometer (Flow Cytometer) is the device that cell is automatically analyzed and sorted, it can be quick
Feature in terms of measurement, storage, a series of important biophysics for showing the cell dispersion to float on a liquid, biochemistry
Parameter, and specified cell subsets can therefrom be sorted out according to the parameter ranges of pre-selection.Sound wave cell sorting techniques are
In recent years the fluidic cell focusing technology occurred.When target cell is by laser detection position, by the fluorescence for detecting its surface
Label, system send instruction to acoustic module, and acoustic module transmits to a sound wave pulse, arteries and veins when target cell passes through
Rush sound wave effect to push it against in corresponding passage in the target cell and by acoustic radiation force, so as to fulfill the sorting of cell.
But due to the limitation of the conditions such as equipment volume, duct width, the prior art is often using surface wave as cell
Sound wave is sorted, i.e., obtains microchannel by being processed on piezoelectric chip, and interdigital electrode is plated in pipeline both sides, obtains surface
Wave device.
However, the limitation of Surface wave technology is very much, such as：First, the material of microchannel must be piezo-electric crystal, and use
During piezo-electric crystal, the ultrasonic energy that ultrasound source produces, there occurs larger loss, is being focused on, divided after piezo-electric crystal
Capacity usage ratio is low during choosing.At present only a few materials such as lithium niobate disclosure satisfy that associated acoustic and optical characteristics will
Ask, such material price is expensive, and acoustical behavior is bad.Second, the limitation of acoustic wave energy, surface wave is compared with bulk wave, hair
The acoustic wave energy penetrated is often smaller, can not be applied to the occasion of high throughput high concentration to the force limited of cell.3rd, sound
Wave impulse is limited with width, and in order to improve the specificity of cell, sound wave pulse energy should be high, and sound wave pulse width is again
It is narrow, and surface wave impulse is often difficult to reach requirement.
Utility model content
In view of this, the utility model embodiment provides a kind of ultrasonic flow cytometer, to solve existing fluidic cell
The problem of ultrasound source capacity usage ratio of instrument is low.
The utility model provides a kind of ultrasonic flow cytometer, including：Sample cell, sheath liquid pipe, flow chamber, the first ultrasound swash
Light converter and the second ultrasonic laser transducer, wherein：
Sample cell is used to store sample liquid；Sheath fluid pipe is used to store sheath fluid；Flow chamber includes the focal zone passage of connection and divides
Two the first branch pipes are extended in constituency passage, the one end of focal zone passage away from sorting area's passage, the first branch pipe respectively with sample
Pipe is connected with sheath liquid pipe；First ultrasonic laser transducer is arranged on the outside of the passage of focal zone, for being produced in the passage of focal zone
First sound wave；Second ultrasonic laser transducer is arranged on the outside of sorting area's passage, for producing the rising tone in sorting area's passage
Ripple.
Alternatively, the length ratio of focal zone passage and sorting area's passage is 1：1~3：1.
Alternatively, sort the one end of area's passage away from focal zone passage and each extend over out the second branch pipe and the 3rd branch pipe, the
Two branch pipes connect the first holder, and the 3rd branch pipe connects the second holder.
Alternatively, the 3rd branch pipe is opened on the tube wall of the second branch pipe.
Alternatively, the cross section of focal zone passage and sorting area's passage is circle.
Alternatively, focal zone channel internal diameter and sorting area's channel internal diameter ratio are 1：1~1：3.
Alternatively, the internal diameter of the second branch pipe is identical with the internal diameter for sorting area's passage.
Alternatively, the 3rd bore and sorting area's channel internal diameter ratio are 1：2~1：4.
Alternatively, the length ratio of the second branch pipe and the 3rd branch pipe is 1：1~1：3.
Alternatively, detection device is further included, for being counted respectively to the second branch pipe and the cell in the 3rd branch pipe.
Ultrasound flow cytometer provided by the utility model, the laser sent using ultrasonic laser transducer are poly- so as to produce
Burnt sound field and standing-wave sound field, are focused cell, sort, the ultrasonic wave produced compared to prior art setting piezo-electric crystal,
Avoid ultrasonic energy and loss is produced after the piezoelectric by high acoustic resistance so that more ultrasonic energies enter detection mesh
Mark, improves ultrasound source capacity usage ratio.Meanwhile the ultrasonic wave that produces of ultrasonic laser transducer has that pulse is short, energy is strong
The characteristics of, real cell can be sorted under high flow velocities, sorting accuracy is high and efficient.In addition, ultrasonic laser
Transducer can also be adjusted the intensity and pulsewidth of ultrasonic wave by adjusting different pumping signals, applied widely.
Brief description of the drawings
Can be more clearly understood the feature and advantage of the utility model by reference to attached drawing, attached drawing be schematically without
It is interpreted as carrying out any restrictions to the utility model, in the accompanying drawings：
Fig. 1 shows a kind of structure diagram of flow cytometer systems in the utility model embodiment 1；
Fig. 2 shows the principle schematic of another flow cytometer in the utility model embodiment 2；
Reference numeral：
1- sample cells；2- sheath liquid pipes；3- flow chambers；31- focal zones passage；32- sorts area's passage；The first branch pipes of 33-；
The second branch pipes of 34-；The 3rd branch pipes of 35-；41- the first ultrasonic laser transducers；42- light-absorption layers；43- extension layers；The ultrasounds of 51- second
Laser transducer；The first holders of 6-；The second holders of 7-；8- detection devices.
Embodiment
It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is the utility model part of the embodiment, instead of all the embodiments.Based on the implementation in the utility model
Example, those skilled in the art's all other embodiments obtained without creative efforts, belongs to this reality
With novel protected scope.
, it is necessary to which explanation, term " first ", " second ", " the 3rd " are only used for describing in the description of the utility model
Purpose, and it is not intended that instruction or hint relative importance.In the accompanying drawings, for clarity, layer and region can be exaggerated
Size and relative size.It should be appreciated that when element such as layer, region or substrate are referred to as " being formed in " or " being arranged on " separately
One element " on " when, which can be arranged directly on another element, or there may also be intermediary element.On the contrary,
When element is referred to as on " being formed directly into " or " being set directly at " another element, there is no intermediary element.
In addition, as long as technical characteristic involved in the utility model different embodiments disclosed below is each other
Not forming conflict can be combined with each other.
Embodiment 1
A kind of ultrasonic flow cytometer is provided in the present embodiment, as shown in Figure 1, the ultrasound flow cytometer includes：For
The sample cell 1 of sample liquid is stored, for storing the sheath liquid pipe 2 of sheath fluid, flow chamber 3；And the first ultrasonic laser transducer 41 and
Two ultrasonic laser transducers 51.Wherein：
Flow chamber 3 includes the focal zone passage 31 and sorting area's passage 32 of connection, and focal zone passage 31 leads to away from sorting area
Two the first branch pipes 33 are extended in the one end in road 32, and the first branch pipe 33 is connected with sample cell 1 with sheath liquid pipe 2 respectively.
First ultrasonic laser transducer 4 is arranged on the outside of focal zone passage 31, for producing the in focal zone passage 31
One sound wave；Second ultrasonic laser transducer 5 is arranged on 32 outside of sorting area's passage, for producing second in sorting area's passage 32
Sound wave.
First ultrasonic laser transducer 4 and the second ultrasonic laser transducer 5 are single wavelength laser, are selected from, but not limited to,
Nd：YAG laser, multi-wavelength tunable pulse laser are such as OPO pulse lasers, semiconductor laser and modulation pulse
It is one or more in LED, short pulse is produced by the laser independent drive of single wavelength or the laser common activation of multiple wavelength and is swashed
Light.As one embodiment of the utility model, in the present embodiment, the first ultrasonic laser transducer 4 is changed with the second ultrasonic laser
Energy device 5 is Nd：YAG laser.The short-pulse laser that first ultrasonic laser transducer 41 produces, shines focal zone passage 31
Penetrate to form sound field, specifically, in focal zone, the outside of passage 31 sets focusing acoustic lenses form or Fresnel to focus on the suction of loop type
Photosphere 42 so that laser produces focusing acoustic field, therefore the cell to be sorted in sample cell 1 and sheath liquid pipe after being irradiated to light-absorption layer 41
In sheath fluid mixing after in flow chamber 3 directed flow, when by focal zone passage 31, cell to be sorted can be in focusing acoustic field
Active force under, convergence it is in alignment.The laser that second ultrasonic laser transducer 51 produces is by sorting 32 peripheral hardware of area's passage
Standing-wave sound field is formed after the light-absorption layer 42 put, since cell is subject to the size and cell itself of acoustic radiation force in by ultrasonic standing wave field
The cube of radius is directly proportional, and the cell of different radii is subject to different size of acoustic radiation force to generate when passing through standing-wave sound field
Deflection, so as to fulfill the sorting of sample.
As one embodiment of the utility model, in the present embodiment, as shown in Figure 1, leading in light-absorption layer 42 with focal zone
There is extension layer 43 between road 31, sorting area's passage 32.Wherein light-absorption layer 42 is made of carbon particle or fiber with gold nano grain,
Or it is made of other metals or non-metallic particle and fiber；Extension layer 43 by the preferable material of acoustic elasticity, such as silica gel, rubber and
Epoxy resin one or more of which forms.
In alternative embodiments, the length ratio of focal zone passage 31 and sorting area's passage 32 is 1：1~3：1, focus on
Passage 31 internal diameter in area's is 1 with 32 internal diameter ratio of sorting area's passage：1~1：3.As one embodiment of the utility model, this implementation
In example, the length ratio of focal zone passage 31 and sorting area's passage 32 is 2：1, passage 31 internal diameter in focal zone is with sorting in area's passage 32
Footpath ratio is 1：2.
As one embodiment of the utility model, in the present embodiment, sorting area's passage 32 is away from focal zone passage 31
One end each extends over out the second branch pipe 34 and the 3rd branch pipe 35, and the second branch pipe 34 connects the first holder 6, and the 3rd branch pipe 35 connects
Second holder 7.First holder 6, the second holder 7 further include the pipeline being connected with the second branch pipe 34, the 3rd branch pipe 35.The
Three branch pipes 35 are opened on the tube wall of the second branch pipe 34.As shown in Figure 1, the 3rd branch pipe 35 is located at the lower section of the second branch pipe 34, it is used for
Receive the less cell of radius.The cross section of focal zone passage 31 and sorting area's passage 32 is circle.The internal diameter of second branch pipe 34
It is identical with the internal diameter for sorting area's passage 32.
3rd branch pipe, 35 internal diameter is 1 with 32 internal diameter ratio of sorting area's passage：2~1：4.An implementation as the utility model
, in the present embodiment, three branch pipes, 35 internal diameter is 1 with 32 internal diameter ratio of sorting area's passage：3.Since the less cell of radius is by standing wave
The acoustic radiation force of sound field is smaller, and the deflection that occurs is smaller when flowing through sorting area's passage 32, can be influenced to circulate downwards by gravity, because
The internal diameter of the pipeline that pipeline that the less cell of this these radius the passes through cell bigger than radius passes through is small.
The length ratio of second branch pipe 34 and the 3rd branch pipe 35 is 1：1~1：3；An implementation as the utility model
, in the present embodiment, the length ratio of the second branch pipe 34 and the 3rd branch pipe 35 is 1：2.
As one embodiment of the utility model, in the present embodiment, detection device 8 is further included, for respectively to second
Branch pipe 34 is counted with the cell in the 3rd branch pipe 35.As shown in Figure 1, detection device is respectively in the second branch pipe 34 and holder
Between 6, between the 3rd branch pipe 35 and holder 7, the cell by sorting is counted, is detected.
The ultrasonic flow cytometer provided in the present embodiment, the laser sent using ultrasonic laser transducer are poly- so as to produce
Burnt sound field and standing-wave sound field, are focused cell, sort, the ultrasonic wave produced compared to prior art setting piezo-electric crystal,
Avoid ultrasonic energy and loss is produced after the piezoelectric by high acoustic resistance so that more ultrasonic energies enter detection mesh
Mark, improves ultrasound source capacity usage ratio.
Embodiment 2
A kind of ultrasonic flow cytometer is provided in the present embodiment, difference lies in sorting area's passage 32 the with embodiment 1
Two ultrasonic laser transducers 5 produce pulsed sound and cell are sorted.
Specifically, detection device 8 is detected before area's passage 32 is sorted, when being detected with target cell or cell will
During by sorting area's passage 32, laser transducer 51 being sent and is instructed, laser transducer is sorted area's passage to target area
32 send sorting pulse, and under the dependent narrow pulse acoustic wave effect of high-energy, target cell or cell are because being subject to its radius three times
The directly proportional acoustic radiation force in side and be moved to corresponding outlet, so as to fulfill the sorting of cell.With reference to figure 2, due to burst pulse sound
Wave energy is than standing-wave sound field energy higher, the larger cell stress bigger of radius, plus be even larger than after buoyancy the cell by
The gravity arrived, therefore the second branch pipe 34 is opened in 32 top of sorting area's passage.
As a variant embodiment of the utility model, in the present embodiment, the first ultrasonic laser transducer is with the second surpassing
Laser used in sound laser transducer is same, and the laser for being produced laser using beam splitter and irradiation system is shone at the same time
It is mapped to two regions；And frequency, the Wavelength tunable of ultrasonic laser transducer, only need to adjust its frequency, wavelength parameter, it becomes possible to
Continue the cell of sorting different radii.
Further, since sound wave pulse energy can be very high, cell or other can be still realized under high flow velocities at this time
The sorting of cell, substantially increases the efficiency of sorting.
Although being described in conjunction with the accompanying the embodiment of the utility model, those skilled in the art can not depart from this
Various modification can be adapted in the case of the spirit and scope of utility model and modification, and such modifications and variations are each fallen within by appended power
Profit is required within limited range.
Claims (10)
- A kind of 1. ultrasound flow cytometer, it is characterised in that including：Sample cell (1), for storing sample liquid；Sheath liquid pipe (2), for storing sheath fluid；Flow chamber (3), including the focal zone passage (31) of connection and sorting area's passage (32), the focal zone passage (31) is remote Two the first branch pipes (33) are extended in one end of sorting area's passage (32), first branch pipe (33) respectively with the sample Pipe (1) is connected with the sheath liquid pipe (2)；First ultrasonic laser transducer (41), is arranged on the outside of the focal zone passage (31), in the focal zone passage (31) the first sound wave is produced in；Second ultrasonic laser transducer (51), is arranged on the outside of sorting area's passage (32), in sorting area's passage (32) the second sound wave is produced in.
- 2. ultrasound flow cytometer according to claim 1, it is characterised in that the focal zone passage (31) and described point The length ratio of constituency passage (32) is 1：1~3：1.
- 3. ultrasound flow cytometer according to claim 2, it is characterised in that sorting area's passage (32) is away from described One end of focal zone passage (31) each extends over out the second branch pipe (34) and the 3rd branch pipe (35), the second branch pipe (34) connection First holder (6), the 3rd branch pipe (35) connect the second holder (7).
- 4. ultrasound flow cytometer according to claim 3, it is characterised in that the 3rd branch pipe (35) is opened in described On the tube wall of second branch pipe (34).
- 5. according to claim 1-4 any one of them ultrasound flow cytometers, it is characterised in that the focal zone passage (31) Cross section with sorting area's passage (32) is circle.
- 6. ultrasound flow cytometer according to claim 5, it is characterised in that focal zone passage (31) internal diameter and institute It is 1 to state sorting area's passage (32) internal diameter ratio：1~1：3.
- 7. ultrasound flow cytometer according to claim 3, it is characterised in that the internal diameter of second branch pipe (34) and institute The internal diameter for stating sorting area's passage (32) is identical.
- 8. it is according to claim 3 ultrasound flow cytometer, it is characterised in that the 3rd branch pipe (35) internal diameter with it is described It is 1 to sort area's passage (32) internal diameter ratio：2~1：4.
- 9. the ultrasonic flow cytometer according to claim 7 or 8, it is characterised in that second branch pipe (34) with it is described The length ratio of 3rd branch pipe (35) is 1：1~1：3.
- 10. ultrasound flow cytometer according to claim 9, it is characterised in that detection device (8) is further included, for dividing It is other that second branch pipe (34) is counted with the cell in the 3rd branch pipe (35).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721201088.9U CN207336317U (en) | 2017-09-19 | 2017-09-19 | A kind of ultrasound flow cytometer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721201088.9U CN207336317U (en) | 2017-09-19 | 2017-09-19 | A kind of ultrasound flow cytometer |
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| CN207336317U true CN207336317U (en) | 2018-05-08 |
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| CN201721201088.9U Active CN207336317U (en) | 2017-09-19 | 2017-09-19 | A kind of ultrasound flow cytometer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109540771A (en) * | 2018-12-18 | 2019-03-29 | 武汉大学 | A kind of the acousto-optic micro-fluidic chip and its method for separating of accurate sorting leukocyte sub-type |
| CN110093271A (en) * | 2019-06-06 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Cell sorting device |
-
2017
- 2017-09-19 CN CN201721201088.9U patent/CN207336317U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109540771A (en) * | 2018-12-18 | 2019-03-29 | 武汉大学 | A kind of the acousto-optic micro-fluidic chip and its method for separating of accurate sorting leukocyte sub-type |
| CN110093271A (en) * | 2019-06-06 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Cell sorting device |
| CN110093271B (en) * | 2019-06-06 | 2024-06-11 | 中国科学院苏州生物医学工程技术研究所 | Cell sorting device |
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