CN210215391U - Cell sorting device - Google Patents
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- CN210215391U CN210215391U CN201920852038.XU CN201920852038U CN210215391U CN 210215391 U CN210215391 U CN 210215391U CN 201920852038 U CN201920852038 U CN 201920852038U CN 210215391 U CN210215391 U CN 210215391U
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Abstract
The utility model discloses a cell sorting device, which is provided with a flow passage structure, wherein the flow passage structure comprises a main flow passage, a sample inlet, a waste liquid port and a collecting port, the downstream of the main flow passage is divided into three branches, a middle branch is connected with the waste liquid port, and two symmetrical branches at two sides are connected with the collecting port; a focusing area, a detection area and a screening area are sequentially arranged on the main runner in the flow direction; the focusing area is used for focusing the particles in the main flow channel in a three-dimensional space, the detection area is used for detecting target particles, and the screening area is used for sorting the target particles and non-target particles. The utility model discloses a sound wave focuses on the cell, saves sheath liquid, and the sample flux can promote. The flow rate is reduced, which is beneficial to the improvement of the subsequent detection sensitivity; the utility model discloses a cell sorting unit is to the cell not damaged, can be used to conventional flow cell and select separately, also can be used to the screening of rare cell, does not have the antenatal examination of wound, tumour prognosis to the promotion and detects very important meaning.
Description
Technical Field
The utility model relates to a biological particle detects and controls technical field, in particular to cell sorting unit.
Background
The detection and capture of rare cells in blood is very little, which is helpful for early diagnosis of diseases and disease monitoring of patients. The existing flow cytometry sorting instrument has the problems of large volume, complex structure, repeated pipeline cleaning, labor and time consumption; and the sorting process is finished in the air, the system is open, aerosol pollution of samples containing cells, bacteria, viruses and the like can be generated, and the clinical application of the aerosol is limited. Currently, most cell sorting systems of BD and Beckman Coulter and the like adopt Jet-in-Air electrostatic deflection separation (U.S. Pat. Nos. 3710933 and 3826364), and although cells can be separated at high speed, the cells are damaged due to high fluid shearing force, and the activity and gene expression of the cells are influenced. For example, in regenerative cell therapy and stem cell research, the survival rate of cells sorted by using the traditional electrostatic cell sorter is low. Meanwhile, in cell regeneration, transgenic samples or virus/bacteria infected sample research, ensuring the tightness and sterility of the environment is a very critical problem. High throughput screening is important for rare cell sorting. The existing flow sorter has limitation on sample introduction flux due to the adoption of sheath fluid focusing, generally less than 100 mu L/min, and the content of rare cells in a sample is low, and a large sample volume is usually required for enrichment, so that the efficient extraction within the cell survival time makes the improvement of the sample flow particularly important.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to the not enough among the above-mentioned prior art, provide a cell sorting unit.
In order to solve the technical problem, the utility model discloses a technical scheme is: a cell sorting device is provided with a flow channel structure, wherein the flow channel structure comprises a main flow channel, a sample inlet, a waste liquid port and a collection port, the downstream of the main flow channel is divided into three branches, a middle branch is connected with the waste liquid port, and two symmetrical branches on two sides are connected with the collection port; a focusing area, a detection area and a screening area are sequentially arranged on the main flow channel along the flow direction;
the focusing area is used for realizing the focusing of particles in the main flow channel in a three-dimensional space, the detection area is used for detecting target particles, and the screening area is used for realizing the sorting of the target particles and non-target particles.
Preferably, at least one piezoelectric transducer unit adhered to the main flow channel is arranged on the focusing area, and is used for focusing the particles in the three-dimensional space in the main flow channel on a central line of the main flow channel or a straight line near the central line of the main flow channel, so that the particles are sequentially and singly arranged and flow in the main flow channel; the screening area is provided with a piezoelectric transducer unit which forms two standing wave nodal lines in the width range of the main flow channel and is used for enabling target particles to flow into two symmetrical branch circuits on two sides of the width direction of the main flow channel under force.
Preferably, the focusing region is provided with 2 piezoelectric transducer units bonded on the main flow channel: the screening device comprises a first piezoelectric transducer unit and a second piezoelectric transducer unit, wherein the piezoelectric transducer unit arranged in the screening area is a third piezoelectric transducer unit;
the flow direction of the main flow channel is taken as an X axis, the width direction of the main flow channel is taken as a Y axis, and the depth direction of the main flow channel is taken as a Z axis, and the first piezoelectric transducer unit and the second piezoelectric transducer unit are used for realizing the focusing of particles in an XY plane and an XZ plane of the main flow channel; the first piezoelectric transducer unit is arranged in an XY plane or an XZ plane of the main flow channel, the second piezoelectric transducer unit is arranged in the XY plane or the XZ plane of the main flow channel, and the third piezoelectric transducer unit is arranged in the XY plane or the XZ plane of the main flow channel; wherein each piezoelectric transducer unit comprises a single piezoelectric transducer arranged on the outer wall of the main flow channel or 2 piezoelectric transducers oppositely adhered on the outer walls of the two sides of the width or depth direction of the main flow channel.
Preferably, the first piezoelectric transducer unit and the second piezoelectric transducer unit are arranged in two planes which are 90 degrees to each other, the first piezoelectric transducer unit is arranged in an XY plane of the main flow channel, the wavelength of the ultrasonic standing wave generated by the first piezoelectric transducer unit is 2 times of the width of the main flow channel in the Y direction, a standing wave node line is formed in the width range of the main flow channel, the node line is located at the middle position of the width of the main flow channel, and is used for focusing the particles on the XY plane; the second piezoelectric transducer unit is arranged in an XZ plane of the main flow channel, the wavelength of ultrasonic standing waves generated by the second piezoelectric transducer unit is 2 times of the depth of the main flow channel in the Z direction, a standing wave pitch line is formed in the depth range of the main flow channel, the position of the pitch line is the middle position of the depth of the main flow channel, and the standing wave pitch line is a sound wave with continuous action and used for focusing particles in the XZ plane.
Preferably, the wavelength of the ultrasonic standing wave generated by the third piezoelectric transducer unit is equal to the width of the main flow channel in the Y direction, two standing wave nodal lines symmetrically distributed along the center line of the main flow channel are formed in the width range of the main flow channel, and the standing wave nodal lines are sound waves with pulse action and are used for enabling target particles to flow along the two standing wave nodal lines under force and enter two symmetrical branch paths on two sides of the main flow channel in the width direction.
Preferably, the first piezoelectric transducer unit and the second piezoelectric transducer unit are arranged in an XY plane or an XZ plane, one of which is used to achieve focusing of the particles in the XY plane and the other is used to achieve focusing of the particles in the XZ plane.
Preferably, when the width of the main channel is equal to the depth of the main channel, the first piezoelectric transducer unit or the second piezoelectric transducer unit is omitted from the focusing area, and the particles are focused in the XY plane and the XZ plane simultaneously by only one piezoelectric transducer unit.
Preferably, the two symmetrical branches join the collecting port after merging at the ends.
Preferably, the two symmetrical branches do not merge at the ends, being connected to two collection ports, respectively.
Preferably, the fluid in the primary channel is kept in a laminar state at all times.
The utility model discloses at least, include following beneficial effect:
1. the utility model utilizes laser detection to judge whether the target is present or not, and carries out target screening at the downstream, even if the physical properties of the cells are similar, the immunofluorescence characteristic can be utilized to carry out effective differentiation;
2. the utility model adopts the piezoelectric element to excite the sound wave in the screening area to push the target cells in the fluid to deviate from the original path, thus realizing cell sorting quickly, and simultaneously, the mechanical force acts on the cells, thus not influencing the activity of the cells;
3. the utility model discloses a sound wave focuses on the cell, saves sheath liquid, and the sample flux can promote. The flow rate is reduced, which is beneficial to the improvement of the subsequent detection sensitivity;
4. the flow passage has simple structure and small flow openings, and is favorable for discharging bubbles and maintaining the stability of the fluid environment;
5. the utility model adopts the mode of leading out the flow port from the side surface, thereby avoiding dead angles of the flow channel, reducing residue and reducing cross contamination among samples;
6. the utility model is formed by bonding and adhering two layers of glass, plastic, metal or polymer materials containing micro pipelines, has an integral structure which is aseptically sealed, can be applied to samples with biohazard, can be used when being plugged, and can discard the sorting chip after being used once;
7. the utility model discloses a cell sorting unit is to the cell not damaged, can be used to conventional flow cell and select separately, also can be used to the screening of rare cell, does not have the antenatal examination of wound, tumour prognosis to the promotion and detects very important meaning.
Drawings
FIG. 1 is a schematic structural view of a cell sorting apparatus according to example 1 of the present invention;
fig. 2 is a schematic structural view of a cell sorting apparatus according to example 2 of the present invention;
fig. 3 is a schematic structural view of a cell sorting apparatus according to example 3 of the present invention;
fig. 4 is a schematic structural view of a cell sorting apparatus according to embodiment 4 of the present invention;
fig. 5 is a schematic block diagram of the detection module of the present invention.
Description of reference numerals:
1-a main runner; 2-sample inlet; 3-waste liquid port; 4-a collection port; 5-a focusing region; 6-detection zone; 7-screening area; 8-a first piezoelectric transducer unit; 9-a second piezoelectric transducer unit; 10-a third piezoelectric transducer cell; 11-laser detection point; 12-target particles; 13-non-target particles.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can implement the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1-5, the cell sorting apparatus of this embodiment has a flow channel structure, the flow channel structure includes a main flow channel 1, a sample inlet 2, a waste liquid port 3 and a collection port 4, the downstream of the main flow channel 1 is divided into three branches, a middle branch is connected to the waste liquid port 3, and two symmetrical branches on two sides are connected to the collection port 4; a focusing area 5, a detection area 6 and a screening area 7 are sequentially arranged on the main runner 1 along the flow direction;
the focusing region 5 is used for focusing particles in the primary channel 1 in three-dimensional space, the detection region 6 is used for detecting target particles, and the screening region 7 is used for sorting target particles 12 and non-target particles 13. The detection area 6 adopts laser detection to judge whether the particles at the laser detection point 11 are target particles, and the piezoelectric transducer unit of the screening area 7 acts according to the judgment result of the detection area 6. The utility model discloses utilize the supersound to carry out high flux granule and select separately, realize the target screening through the detection of laser to the granule.
The utility model discloses in, cell sorting unit can be a sorting chip, and the runner structure is the microtube way, forms inside sorting chip. The runner structure accessible substrate and cover plate form, and the pipeline recess has been seted up to the cover plate bottom, and the cover plate is sealed to be attached to on the substrate, makes the pipeline recess that is located between cover plate and the piezoelectricity substrate form the runner structure. The base sheet and the cover sheet may be made of glass, plastic, metal or polymer. The whole structure is sterile and sealed, can be suitable for samples with biohazard, can be used in a plug-and-play mode, and can be discarded after being used once. In another embodiment, the main channel 1 in the channel structure is formed by drawing a material and is connected to the subsequent branch channels. In a preferred embodiment, each of the channels in the flow channel structure has a width of 10-500 μm and a height of 20-200 μm. The fluid in the flow channel structure is always kept in a laminar flow state.
Before the sample cells enter the cell sorting device, the fluorescence staining treatment of the target cells is carried out. The target cells are stained (by adding a specific antibody which is fluorescently labeled and can be specifically combined with the target cells), and the flow fluorescence detection technology is utilized to detect the fluorescent dye on the specific antibody combined with the target cells at a laser detection point, so that the target cells are identified, and the target cells are extracted.
The detection area 6 can specifically realize detection through the following scheme: the detection module on the detection area 6 comprises a light spot excitation modulation system, an optical signal detection system, a data acquisition analysis and control module and a piezoelectric driving module; the light spot excitation modulation system generates laser and forms an elliptical light spot, the elliptical light spot irradiates a fixed position on the main flow channel 1 of the detection area 6, and each cell flowing through the detection area 6 is irradiated and excited to generate fluorescence and scattered light; the optical signal detection system collects and detects the generated fluorescence and scattered light to form optical signal information, and the obtained optical signal information is converted into an electric signal and then is sent to the data acquisition analysis and control module. The data acquisition, analysis and control module quantizes and analyzes the received electric signals, logically judges the processing result and the screening condition set by the user, and sends a screening trigger signal to the piezoelectric driving module of the screening area 7 if the screening condition set by the user is met; the piezoelectric driving module converts the screening trigger signal into a high-voltage signal for driving a third piezoelectric transducer unit in the screening area 7, so that the high-voltage signal generates a sound field to change the motion path of the target cell, and the target cell and the non-target cell are separated to different sorting outlets, thereby realizing cell sorting.
The focusing area 5 is provided with at least one piezoelectric transducer unit bonded on the main flow channel 1 and used for focusing the particles in the three-dimensional space in the main flow channel 1 on the central line of the main flow channel 1 or on a straight line near the central line of the main flow channel 1 so that the particles are sequentially and singly arranged and flow in the main flow channel 1; the screening area 7 is provided with a piezoelectric transducer unit which forms two standing wave nodal lines in the width range of the main flow channel 1 and is used for enabling target particles to flow into two symmetrical branches at two sides of the main flow channel 1 in the width direction under force.
Because the screening area 7 is located at the downstream of the detection area 6, the trigger signal needs to be generated after waiting for a certain cell flow time, when the cell moves to the position of the downstream screening area 7 from the detection area 6, the piezoelectric ceramic driving module generates a driving signal with a certain pulse width and a certain voltage, and the signal controls the generation of a sound field, so that the target cell is pushed, and the purpose of separating the cell according to the difference of the detected optical signals is realized. Wherein, the amplitude of the driving voltage and the deformation of the piezoelectric ceramic are in positive correlation, and the higher the voltage is, the larger the thrust is.
When the screening area 7 judges the target cells, a positive selection method or a negative selection method is adopted, and when the positive selection method is adopted, signals which accord with the characteristics of the target cells are used as judgment basis, the third piezoelectric transducer is controlled to generate actions, namely, the detected positive values are used as gating basis, gates are arranged in the detected positive value area, and when the detection signals fall into the gates, the third piezoelectric transducer generates actions, so that the target particles are forced to flow into the collection port 4. When the reverse selection method is adopted, a signal which accords with the characteristics of non-target cells is used as a judgment basis, the third piezoelectric transducer is controlled to act, namely, the detected positive value is used as a gating basis, a gate is arranged outside the area of the detected positive value, and when the detection signal falls into the gate, the third piezoelectric transducer acts to force target particles to flow into the collection port 4. Taking the screening of fetal nucleated red blood cells in the peripheral blood of pregnant women as an example, when the positive selection method is adopted, the arrival of target cells can be judged according to the positive signal of CD71 (fetal nucleated red blood cell specific antibody), so that the action of the piezoelectric transducer is controlled. In the case of the counter selection method, the arrival of the target cell can be determined based on a negative signal of CD45 (leukocyte-specific antibody), and the operation of the piezoelectric transducer can be controlled. When the antibody specificity of the target cell is not good, the capture rate can be improved by adopting a counter selection method.
The focusing area 5 is provided with 2 piezoelectric transducer units bonded on the main flow passage 1: the piezoelectric transducer unit arranged in the screening area 7 is a third piezoelectric transducer unit;
the first piezoelectric transducer unit 8 and the second piezoelectric transducer unit 9 are used for realizing the focusing of particles in an XY plane and an XZ plane of the main flow channel 1 by taking the flow direction of the main flow channel 1 as an X axis, the width direction of the main flow channel 1 as a Y axis and the depth direction of the main flow channel 1 as a Z axis; the first piezoelectric transducer unit 8 is arranged in the XY plane or XZ plane of the main flow channel 1, the second piezoelectric transducer unit 9 is arranged in the XY plane or XZ plane of the main flow channel 1, and the third piezoelectric transducer unit is arranged in the XY plane or XZ plane of the main flow channel 1; wherein, each piezoelectric transducer unit comprises a single piezoelectric transducer arranged on the side wall of the main runner, or 2 piezoelectric transducers (namely a piezoelectric transducer group) oppositely adhered on the outer walls of the two sides of the width or depth direction of the main runner. The first piezoelectric transducer unit 8 and the second piezoelectric transducer unit 9 can be switched in position, can be arranged on the same plane, can also be arranged on different planes, and only the cooperation of the first piezoelectric transducer unit and the second piezoelectric transducer unit can realize the focusing of particles in an XY plane and an XZ plane.
The wavelength of the ultrasonic standing wave generated by the third piezoelectric transducer unit is equal to (or approximately equal to) the width of the main channel 1Y direction, two standing wave nodal lines which are symmetrically distributed along the central line of the main channel 1 are formed in the width range of the main channel 1, and the standing wave nodal lines are sound waves with pulse action, are used for enabling target particles to flow along the two standing wave nodal lines under the action of ultrasonic radiation force and enter two symmetrical branch circuits on two sides of the main channel 1 in the width direction, and finally enter the collection port 4. The third piezoelectric transducer unit has a short action time so as to enable the target cell to generate enough deviation relative to the original flow path as a measuring and calculating basis.
In order to reduce the formation of dead angles of the flow path, a sample injection mode can be adopted on the side surface of the flow path (namely, an inlet and an outlet are led out on the side surface of the flow path), so that the cross contamination of the detection sample can be reduced.
Wherein, the fluid in the main channel 1 is always kept in a laminar state.
Referring to the figure, in the present embodiment, two symmetrical branches are combined at the ends and then connected to the collecting port 4. In another embodiment, however, the two symmetrical branches do not merge at the ends and are each connected to two collection ports 4. Two collection ports 4 can be led out from the side of the whole fluidic chip, which is also beneficial to reducing the cross contamination of the detection samples.
Several specific examples are provided below to further illustrate the present invention.
Example 1
Referring to fig. 1, in the present embodiment, a first piezoelectric transducer unit 8 and a second piezoelectric transducer unit 9 are disposed in two planes at 90 ° to each other, the first piezoelectric transducer unit 8 is disposed in an XY plane of a main flow channel 1, the wavelength of an ultrasonic standing wave generated by the first group of piezoelectric transducers is 2 times (or about 2 times) the width of the main flow channel 1 in the Y direction, a standing wave pitch line is formed in the width range of the main flow channel 1, the pitch line is located at a middle position of the width of the main flow channel 1 (or close to the middle position of the width of the main flow channel 1), and is a continuously acting sound wave for focusing particles on the XY plane, that is, the particles move in a single row in the XY plane; the second piezoelectric transducer unit 9 is disposed in an XZ plane of the main channel 1, the wavelength of the ultrasonic standing wave generated by the second group of piezoelectric transducers is 2 times (or about 2 times) the depth of the main channel 1 in the Z direction, a standing wave node line is formed in the depth range of the main channel 1, the node line is located at a middle position of the depth of the main channel 1 (or a middle position close to the width of the main channel 1), and is a continuously acting sound wave for focusing particles in the XZ plane, that is, the particles move in a single row in the XZ plane. And wherein the positions of the first piezoelectric transducer unit 8 and the second piezoelectric transducer unit 9 are interchangeable.
Example 2
Referring to fig. 2, in the present embodiment, the first piezoelectric transducer unit 8 and the second piezoelectric transducer unit 9 are each disposed in an XY plane or an XZ plane, one of which is for effecting focusing of particles in the XY plane and the other of which is for effecting focusing of particles in the XZ plane.
Example 3
When the width of the primary channel 1 is equal to its depth, referring to fig. 3, in this embodiment, only one piezoelectric transducer unit is disposed on the focusing region 5, and the focusing of the particles in the XY plane and the XZ plane is simultaneously achieved. By controlling the parameters of the piezoelectric transducer units, the vibration frequency of the main runner wall is adjusted (the vibration frequency is mainly related to the depth and the width of the main runner 1, and when the width of the main runner 1 is equal to the depth, the vibration frequencies of the two side walls of the width and the two side walls of the depth are the same), so that the particles can be focused in an XY plane and an XZ plane through one piezoelectric transducer unit.
Example 4
Since the third group of piezoelectric transducers generates standing waves for a period of time, if both target and non-target particles are present in its active time and active area, it may happen that non-target particles are also pushed into the collection conduit. For this reason, in the present embodiment, referring to fig. 4, the collected particles may be sorted again in a cascade of a plurality of units to improve the purity of the finally obtained target particles. When the cascade mode is adopted, the collection port 4 of the previous stage unit is the sample inlet 2 of the next stage unit.
While the embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields where the invention is suitable, and further modifications may readily be made by those skilled in the art, and the invention is therefore not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.
Claims (10)
1. A cell sorting device is characterized by having a flow channel structure, wherein the flow channel structure comprises a main flow channel, a sample inlet, a waste liquid port and a collection port, the downstream of the main flow channel is divided into three branches, a middle branch is connected with the waste liquid port, and two symmetrical branches on two sides are connected with the collection port; a focusing area, a detection area and a screening area are sequentially arranged on the main flow channel along the flow direction;
the focusing area is used for realizing the focusing of particles in the main flow channel in a three-dimensional space, the detection area is used for detecting target particles, and the screening area is used for realizing the sorting of the target particles and non-target particles.
2. The cell sorting device according to claim 1, wherein the focusing region is provided with at least one piezoelectric transducer unit adhered to the main flow channel for focusing the particles in the three-dimensional space in the main flow channel on a center line of the main flow channel or a straight line near the center line of the main flow channel so that the particles flow in the main flow channel in sequence and individually; the screening area is provided with a piezoelectric transducer unit which forms two standing wave nodal lines in the width range of the main flow channel and is used for enabling target particles to flow into two symmetrical branch circuits on two sides of the width direction of the main flow channel under force.
3. The cell sorting device according to claim 2, wherein the focusing region is provided with 2 piezoelectric transducer units adhered to the primary flow channel: the screening device comprises a first piezoelectric transducer unit and a second piezoelectric transducer unit, wherein the piezoelectric transducer unit arranged in the screening area is a third piezoelectric transducer unit;
the flow direction of the main flow channel is taken as an X axis, the width direction of the main flow channel is taken as a Y axis, and the depth direction of the main flow channel is taken as a Z axis, and the first piezoelectric transducer unit and the second piezoelectric transducer unit are used for realizing the focusing of particles in an XY plane and an XZ plane of the main flow channel; the first piezoelectric transducer unit is arranged in an XY plane or an XZ plane of the main flow channel, the second piezoelectric transducer unit is arranged in the XY plane or the XZ plane of the main flow channel, and the third piezoelectric transducer unit is arranged in the XY plane or the XZ plane of the main flow channel; wherein each piezoelectric transducer unit comprises a single piezoelectric transducer arranged on the outer wall of the main flow channel or 2 piezoelectric transducers oppositely adhered on the outer walls of the two sides of the width or depth direction of the main flow channel.
4. The cell sorting device according to claim 3, wherein the first piezoelectric transducer unit and the second piezoelectric transducer unit are arranged in two planes at 90 ° to each other, the first piezoelectric transducer unit is arranged in an XY plane of the main flow channel, the wavelength of the ultrasonic standing wave generated by the first piezoelectric transducer unit is 2 times the width of the main flow channel in the Y direction, a standing wave pitch line is formed within the width of the main flow channel, the pitch line position is a middle position of the width of the main flow channel, and is a continuously acting sound wave for focusing the particles on the XY plane; the second piezoelectric transducer unit is arranged in an XZ plane of the main flow channel, the wavelength of ultrasonic standing waves generated by the second piezoelectric transducer unit is 2 times of the depth of the main flow channel in the Z direction, a standing wave pitch line is formed in the depth range of the main flow channel, the position of the pitch line is the middle position of the depth of the main flow channel, and the standing wave pitch line is a sound wave with continuous action and used for focusing particles in the XZ plane.
5. The cell sorting apparatus according to claim 3, wherein the wavelength of the ultrasonic standing wave generated by the third piezoelectric transducer unit is equal to the width of the main flow channel in the Y direction, two standing wave nodal lines are formed within the width of the main flow channel, symmetrically distributed along the center line of the main flow channel, and are pulsed acoustic waves for forcing the target particles to flow along the two standing wave nodal lines into two symmetrical branches on both sides of the main flow channel in the width direction.
6. The cell sorting device according to claim 3, wherein the first piezoelectric transducer unit and the second piezoelectric transducer unit are each disposed in an XY plane or an XZ plane, one of which is for effecting focusing of the particles in the XY plane and the other of which is for effecting focusing of the particles in the XZ plane.
7. The cell sorting device according to claim 3, wherein when the width of the main flow channel is equal to the depth thereof, the first piezoelectric transducer unit or the second piezoelectric transducer unit is omitted from the focusing area, and focusing of particles in the XY plane and the XZ plane is simultaneously achieved by only one piezoelectric transducer unit.
8. The cell sorting device according to any one of claims 1 to 7, wherein the two symmetrical branches join the collection port after merging at their ends.
9. The cell sorting device according to any one of claims 1 to 7, wherein the two symmetrical branches do not merge at the ends and are connected to the two collection ports, respectively.
10. The cell sorting device according to any one of claims 1 to 7, wherein the fluid in the main flow channel is always kept in a laminar flow state.
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| CN113218822A (en) * | 2021-04-29 | 2021-08-06 | 宋卓 | Focusing structure, particle detection device comprising focusing structure and use method of particle detection device |
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| CN113218822A (en) * | 2021-04-29 | 2021-08-06 | 宋卓 | Focusing structure, particle detection device comprising focusing structure and use method of particle detection device |
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