CN103048258A - Light splitting device for flow cytometer - Google Patents
Light splitting device for flow cytometer Download PDFInfo
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- CN103048258A CN103048258A CN2012105737833A CN201210573783A CN103048258A CN 103048258 A CN103048258 A CN 103048258A CN 2012105737833 A CN2012105737833 A CN 2012105737833A CN 201210573783 A CN201210573783 A CN 201210573783A CN 103048258 A CN103048258 A CN 103048258A
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Abstract
The invention relates to a light splitting device for a flow cytometer. The light splitting device comprises a laser radiation unit, a flow detection cell, a forward detection unit and a lateral detection unit, wherein a reflecting mirror and a two-color optical filter are respectively arranged in front of two lasers which are arranged in parallel in the laser radiation unit, and a focusing lens is arranged in front of the reflecting mirror and the two-color optical filter; the flow detection cell is arranged behind the focusing lens, and forward detection unit is arranged behind the flow detection cell, and the lateral detection unit is positioned in the lateral direction of the flow detection cell; the lateral detection unit comprises an alignment system and a multiple volume holographic grating which is arranged behind the alignment system and is used for splitting multi-wavelength light; and a lateral scattered light detector and a plurality of fluorescent detectors are positioned behind the multiple volume holographic grating. The light splitting device utilizes a multiple volume holographic grating wavelength filter technique and has the advantages of sensitive wavelength selectivity, good polarization independence, high diffraction efficiency, stable device performance, long life cycle, compact structure, low cost and the like.
Description
Technical field
The present invention relates to a kind of light-dividing device for flow cytometer.
Background technology
Flow cytometer (Flow cytometer, FCM) be the advanced science and technology equipment that light harvesting, electronics, fluid mechanics, cytochemistry, biology, immunology and the multi-door subject technologies such as laser and computing machine are integrated, it is widely used in the fields such as clinical medicine, cytology, biology, microbiology, pharmaceutics, genesiology.Be one of advanced instrument in the modern scientific research, be described as " CT " in laboratory.It to the single-row cell in the quick streamlined flow state or biologic grain carry out one by one, the technology of multiparameter, fast qualitative and quantitative analysis or sorting, have the characteristics such as detection speed is fast, measurement parameter is many, image data is large, analysis is comprehensive, separating purity is high, method is flexible.The beam splitting system that is used for flow cytometer on the prior art mainly adopts optical splitter and optical filter to form, so that system architecture is complicated, volume is large, and cost is high.
Summary of the invention
Purpose of the present invention proposes a kind of simple in structure, stable performance, cheap exactly, can carry out to a plurality of wavelength the light-dividing device that is used for flow cytometer of light splitting.
Light-dividing device for flow cytometer of the present invention, comprise laser emission unit, flow detection pond, forward detection unit and side direction detecting unit, two laser instrument the place aheads that are arranged in parallel arrange respectively catoptron and dichroic filter in the laser emission unit, catoptron and dichroic filter all with laser emitting laser angle at 45 °, condenser lens is located at the place ahead of catoptron and dichroic filter, condenser lens, catoptron, dichroic filter three are on same axis, and two-laser becomes 90 ° of angles with condenser lens; The flow detection pond is positioned over the condenser lens rear, the focal beam spot quadrature is radiated on the cell liquid of the detection cell of flowing through, the rear in flow detection pond is the forward detection unit, be used for receiving forward scattering light, the side direction detecting unit is positioned at flow detection pond side direction, become 90 ° of angles with the forward detection unit, the side direction detecting unit comprises colimated light system and is placed on the colimated light system rear and be used for multi-wavelength light is carried out the polyad holographic grating of light splitting, be positioned at all angles position setting side set when writing grating at polyad holographic grating rear to detector for scattered light and a plurality of fluorescent probe.
Light-dividing device for flow cytometer of the present invention utilizes that polyad holographic grating wave length filtering technology has the wavelength selectivity sensitivity, polarization independence is good, diffraction efficiency is high (in theory near 100%), device performance is stable and life cycle length, compact conformation, the advantage such as cheap.
Description of drawings
Fig. 1 is structural representation of the present invention.
Embodiment
A kind of light-dividing device for flow cytometer, comprise laser emission unit 100, flow detection pond 200, forward detection unit 300 and side direction detecting unit 400, the two left laser instruments 110 that are arranged in parallel in the laser emission unit 100, right laser instrument 120 the place aheads arrange respectively catoptron 130 and dichroic filter 140, catoptron 130 and dichroic filter 140 all with laser emitting laser angle at 45 °, condenser lens 150 is located at the place ahead of catoptron 130 and dichroic filter 140, condenser lens 150, catoptron 130, dichroic filter 140 threes on same axis, 150 one-tenth 90 ° of angles of two-laser and condenser lens; Flow detection pond 200 is positioned over condenser lens 150 rears, the focal beam spot quadrature is radiated on the cell liquid of the detection cell 200 of flowing through, the rear in flow detection pond 200 is forward detection unit 300, be used for receiving forward scattering light, side direction detecting unit 400 is positioned at flow detection pond 200 side direction, with the 300 one-tenth 90 ° of angles in forward detection unit, side direction detecting unit 400 comprises colimated light system 410 and is placed on colimated light system 410 rears and be used for multi-wavelength light is carried out the polyad holographic grating 420 of light splitting, is positioned at all angles position setting side set when writing grating at polyad holographic grating 420 rears to detector for scattered light 130 and a plurality of fluorescent probe 440-470.
The laser that sends of the 488nm of the 640nm of left laser instrument 110 and right laser instrument 120 as shown in the figure, catoptron 130 reflections of the laser that its left laser instrument 100 sends by become miter angle with light beam, then by two colo(u)r filters 140, the laser that 640nm sends with right laser instrument 120 by/488nm reflection forms the hot spot of about 22 * 66um by condenser lens 150, and quadrature is radiated at flow detection pond 200 and produces forward scattering light, side scattered light and fluorescence excitation.Place a photodiode 300 as the forward scattering photo-detector in 200 the place aheads, flow detection pond.Place first a collimation lens 410 in 90 ° of directions fluorescence and the side scattered light that ejects become parallel beam, parallel beam impinges perpendicularly in the polyad holographic grating 420 that is placed on behind the colimated light system, by bragg's formula, can determine grating write wavelength of light and read-in angle and read wavelength of light and angle between relation, thereby determine to read angle.Mate in the fluorescence of each wavelength and scattered light and corresponding grating Prague, reads the light of each wavelength from different directions.Then reading light direction at each places with light signal lateral scattering photo-detector 430(photomultiplier) and fluorescent probe (photomultiplier) (440～470) be transformed into electric signal and amplify and can survey the fluorescence signal of each wavelength.
Claims (1)
1. light-dividing device that is used for flow cytometer, comprise laser emission unit, flow detection pond, forward detection unit and side direction detecting unit, it is characterized in that: two laser instrument the place aheads that are arranged in parallel arrange respectively catoptron and dichroic filter in the laser emission unit, catoptron and dichroic filter all with laser emitting laser angle at 45 °, condenser lens is located at the place ahead of catoptron and dichroic filter, condenser lens, catoptron, dichroic filter three are on same axis, and two-laser becomes 90 ° of angles with condenser lens; The flow detection pond is positioned over the condenser lens rear, the rear in flow detection pond is the forward detection unit, the side direction detecting unit is positioned at flow detection pond side direction, become 90 ° of angles with the forward detection unit, the side direction detecting unit comprises colimated light system and is placed on the colimated light system rear, is positioned at all angles position setting side set when writing grating at polyad holographic grating rear to detector for scattered light and a plurality of fluorescent probe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105737833A CN103048258A (en) | 2012-12-26 | 2012-12-26 | Light splitting device for flow cytometer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105737833A CN103048258A (en) | 2012-12-26 | 2012-12-26 | Light splitting device for flow cytometer |
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| CN103048258A true CN103048258A (en) | 2013-04-17 |
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| CN2012105737833A Pending CN103048258A (en) | 2012-12-26 | 2012-12-26 | Light splitting device for flow cytometer |
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Cited By (8)
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|---|---|---|---|---|
| CN103487359A (en) * | 2013-09-25 | 2014-01-01 | 江西科技师范大学 | Full-automatic measuring device for form and distribution of laser excitated cells and particles |
| CN103575712A (en) * | 2013-10-15 | 2014-02-12 | 中国科学院苏州生物医学工程技术研究所 | Particle fluorescence detection wavelength instant configuration beam splitting system |
| CN104155242A (en) * | 2014-07-24 | 2014-11-19 | 太仓能健生物技术有限公司 | Light path device of fluid analysis equipment |
| CN104266955A (en) * | 2014-09-02 | 2015-01-07 | 上海凯度机电科技有限公司 | High content image flow biological microscopic analysis system |
| CN105181561A (en) * | 2015-09-02 | 2015-12-23 | 江苏大学 | Hemocyte analysis sensor |
| CN106680186A (en) * | 2016-11-02 | 2017-05-17 | 北京信息科技大学 | Multi-type scattered light detection system of flow cytometer |
| CN107290265A (en) * | 2017-06-26 | 2017-10-24 | 北京华夏光谷光电科技有限公司 | ultra-wide spectrum multi-channel laser flow cytometer |
| CN110806491A (en) * | 2019-11-15 | 2020-02-18 | 成都宜乐芯生物科技有限公司 | Full-automatic POCT multiple detection liquid phase chip system |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103487359B (en) * | 2013-09-25 | 2016-03-30 | 江西科技师范大学 | A kind of cell of laser excitation and particle shape and apparatus for measuring distribution |
| CN103487359A (en) * | 2013-09-25 | 2014-01-01 | 江西科技师范大学 | Full-automatic measuring device for form and distribution of laser excitated cells and particles |
| CN103575712A (en) * | 2013-10-15 | 2014-02-12 | 中国科学院苏州生物医学工程技术研究所 | Particle fluorescence detection wavelength instant configuration beam splitting system |
| CN104155242A (en) * | 2014-07-24 | 2014-11-19 | 太仓能健生物技术有限公司 | Light path device of fluid analysis equipment |
| CN104266955A (en) * | 2014-09-02 | 2015-01-07 | 上海凯度机电科技有限公司 | High content image flow biological microscopic analysis system |
| CN105181561B (en) * | 2015-09-02 | 2018-04-24 | 江苏大学 | A kind of blood cell analysis sensor |
| CN105181561A (en) * | 2015-09-02 | 2015-12-23 | 江苏大学 | Hemocyte analysis sensor |
| CN106680186A (en) * | 2016-11-02 | 2017-05-17 | 北京信息科技大学 | Multi-type scattered light detection system of flow cytometer |
| CN106680186B (en) * | 2016-11-02 | 2019-03-01 | 北京信息科技大学 | A kind of flow cytometer polymorphic type scattering optical detection system |
| CN107290265A (en) * | 2017-06-26 | 2017-10-24 | 北京华夏光谷光电科技有限公司 | ultra-wide spectrum multi-channel laser flow cytometer |
| CN107290265B (en) * | 2017-06-26 | 2022-06-28 | 北京华夏光谷光电科技有限公司 | Ultra-wide spectrum multi-channel laser flow cytometer |
| CN110806491A (en) * | 2019-11-15 | 2020-02-18 | 成都宜乐芯生物科技有限公司 | Full-automatic POCT multiple detection liquid phase chip system |
| CN110806491B (en) * | 2019-11-15 | 2024-03-29 | 成都宜乐芯生物科技有限公司 | Full-automatic POCT multiple detection liquid phase chip system |
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Application publication date: 20130417 |