CN104155242A - Light path device of fluid analysis equipment - Google Patents
Light path device of fluid analysis equipment Download PDFInfo
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- CN104155242A CN104155242A CN201410355636.8A CN201410355636A CN104155242A CN 104155242 A CN104155242 A CN 104155242A CN 201410355636 A CN201410355636 A CN 201410355636A CN 104155242 A CN104155242 A CN 104155242A
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- light path
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Abstract
The invention discloses a light path device of fluid analysis equipment. The light path device comprises a laser irradiation unit, a fluid tank and a fluorescent light splitting unit, wherein a flowing fluid sample is borne in the fluid tank and contains a fluorescent dye labelled substance to be detected; the laser irradiation unit carries out laser irradiation on the fluid sample in the fluid tank and obtains the labelled fluorescent light through excitation; the fluorescent light is received by a fluorescent light receiver of the fluid analysis equipment after being split by the fluorescent light splitting unit; the fluorescent light splitting unit comprises a corner cube used for dispersing the fluorescent light into a plurality of fluorescent light beams; the corner cube is arranged between the fluid tank and the fluorescent light receiver of the fluid analysis equipment. The invention aims to provide the light path device used for the fluid analysis equipment such as flow cytometers. The light path device can reduce the light energy loss during fluorescent light splitting, improve the fluorescent light collection efficiency and enhance the capacity of processing weak signals of subsequent circuits.
Description
Technical field
the present invention relates to the light path device of a kind of light path device of fluid analysis equipment, particularly a kind of flow cytometer.
Background technology
flow cytometer is that light harvesting, electronics, fluid mechanics, cytochemistry and computer technology are in the accurate biomedical analyzer device of one, for fast quantitative analysis and the sorting of cell.Along with the development of each section's technology, flow cytometer becomes the important tool of biological and medical research, and having higher sensitivity, resolution and more excitation wavelength is flow cytometer R and D personnel's target.Light path device structural representation in conjunction with flow cytometer in the prior art shown in Fig. 1, the multi-path laser that a plurality of laser instruments 10 of flow cytometer produce is (as 405nm, 488nm, 633nm etc.) all adopt laser light combination mirror 20 to close bundle, by light combination mirror 20, realize multi-path laser synthetic, by a shared condenser lens 30, laser beam is focused on to fluid pool 40 inside.Because use identical condenser lens while swashing combiner, cause spectral range wide, need to be to Focused Optical system achromatism, also more difficultly realize all effective utilizations of even luminous energy of focal spot.What phosphor collection optical system adopted at present is all that light receiving microscopy 50 coordinates 60 transmission of the light elements such as optical fiber, although subsequent optical path flexible arrangement is convenient, the subject matter of bringing is that the coupling efficiency of optical fiber end is not high, cause fluorescence signal fainter, the detection of follow-up feeble signal is brought to larger challenge.
Summary of the invention
for the problems referred to above, the object of this invention is to provide a kind of light path device for fluid analysis equipment such as flow cytometers, it can reduce the optical energy loss in fluorescence spectrophotometer, improves phosphor collection efficiency, strengthens the processing power of subsequent conditioning circuit feeble signal.
for solving the problems of the technologies described above, the present invention adopts following technical scheme:
a kind of light path device of fluid analysis equipment, comprise Ear Mucosa Treated by He Ne Laser Irradiation unit, fluid pool, fluorescence spectrophotometer unit, in described fluid pool, carry mobile fluid sample, the determinand that contains fluorochrome label in fluid sample, the fluid sample in described Ear Mucosa Treated by He Ne Laser Irradiation cellular convection body pond carries out Ear Mucosa Treated by He Ne Laser Irradiation, excite the fluorescence that obtains institute's mark, by being received by the fluorescence reception device of this fluid analysis equipment after the unit light splitting of described fluorescence spectrophotometer, described spectrophotometric unit comprises for described fluorescence being separated into the prism of corner cube of a plurality of fluorescence beam, described prism of corner cube is between fluid pool and the fluorescence reception device of this fluid analysis equipment.
preferably, described Ear Mucosa Treated by He Ne Laser Irradiation unit comprises a plurality of laser instruments, and the laser beam that each laser instrument generates is focused on respectively the fluid sample place formation focal beam spot of fluid pool by least one condenser lens, and the wavelength of the laser beam that a plurality of described laser instruments generate is different.
more preferably, this light path device also comprises that one projects the first catoptron on prism of corner cube for the fluorescence that each focal beam spot is inspired, and described in each, laser beam is from the one or both sides of the first catoptron to fluid pool incident.
further, described fluid pool is multiaspect column, the side of described fluid pool comprise reflecting surface, and described reflecting surface over against exit facet, a plurality of planes of incidence between described reflecting surface and exit facet, described the first catoptron is over against reflecting surface setting, described in each, laser beam is respectively from plane of incidence incident, and described prism of corner cube is over against exit facet setting.
further, described fluid pool is octahedral column, and described laser instrument is three, and three laser beam are respectively from being positioned at three plane of incidence incidents of described first catoptron the same side, and described prism of corner cube is Rhizoma Sparganii taper prism of corner cube.
further, described fluid pool is octahedral column, and described laser instrument is six, and six laser beam are respectively from being positioned at six plane of incidence incidents of described the first catoptron both sides, and described prism of corner cube is hexagonal pyramid shape prism of corner cube.
further, described the first catoptron is free-form surface mirror or non-spherical reflector.
preferably, described light path device also comprises diaphragm, and described in each, fluorescence beam is received by fluorescence reception device after removing veiling glare by diaphragm respectively.
preferably, described light path device also comprises collimating mirror, and described in each, fluorescence beam has fluorescence reception device to receive after collimating by collimating mirror respectively.
preferably, described light path device also comprise be arranged at described prism of corner cube sidepiece for adjusting the second catoptron of the transmission direction of fluorescence beam.
the present invention adopts above structure, tool has the following advantages: fluorescence focal beam spot being inspired by prism of corner cube is separated into after a plurality of fluorescence beam by wavelength difference, by the fluorescence reception device of fluid analysis equipment, receive laggard row and detect analysis, realized the projection of many phosphor dots, avoided the use of optical fiber in prior art, optical energy loss in occasionally reducing fluorescence spectrophotometer and collecting, improves phosphor collection efficiency, strengthens the processing power of subsequent conditioning circuit feeble signal.
Accompanying drawing explanation
fig. 1 is the light path device of fluid analysis equipment in prior art;
fig. 2 is the structure intention of the embodiment of the present invention one;
fig. 3 is the structural representation of Rhizoma Sparganii taper prism of corner cube in the embodiment of the present invention one;
fig. 4 is the structural representation of the embodiment of the present invention two.
wherein: 10, laser instrument; 20, light combination mirror; 30, condenser lens; 40, fluid pool; 50, light receiving microscopy; 60, light element;
11, laser instrument; 21, condenser lens; 31, the first catoptron; 41, fluid pool; 51, Rhizoma Sparganii taper prism of corner cube; 61, the second catoptron; 71, diaphragm; 81, collimating mirror;
12, laser instrument; 22, condenser lens; 32, the first catoptron; 42, fluid pool; 52, hexagonal pyramid shape prism of corner cube; 62, the second catoptron; 72, diaphragm; 82, collimating mirror.
Embodiment
below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be those skilled in the art will recognize that, protection scope of the present invention is made to more explicit defining.
embodiment mono-
in conjunction with the light path device that Figure 2 shows that a kind of fluid analysis equipment, in figure, direction shown in arrow is the travel path of laser beam or fluorescence beam.This light path device comprises Ear Mucosa Treated by He Ne Laser Irradiation unit, fluid pool 41, fluorescence spectrophotometer unit, in described fluid pool 41, carry fluid sample, the determinand that contains fluorochrome label in fluid sample, the fluid sample in described Ear Mucosa Treated by He Ne Laser Irradiation cellular convection body pond 41 carries out Ear Mucosa Treated by He Ne Laser Irradiation, excite the fluorescence that obtains institute's mark, by being received by the fluorescence reception device of this fluid analysis equipment after described beam splitting system light splitting, described spectrophotometric unit comprises for described fluorescence being separated into the prism of corner cube of a plurality of fluorescence beam, described prism of corner cube is between fluid pool 41 and the fluorescence reception device of this fluid analysis equipment.The wavelength of a plurality of fluorescence beam is different.
described Ear Mucosa Treated by He Ne Laser Irradiation unit comprises three laser instruments 11, and the laser beam that each laser instrument 11 generates is focused on respectively the liquid stream position formation focal beam spot of fluid pool 41 by a condenser lens 21, and the wavelength of the laser beam of three laser instrument 11 generations is different.The laser beam of addressing laser instrument 11 generations in the present invention all refers to the collimated light beam through shaping and after expanding.Three condenser lenses 21 are for focusing on three laser beam respectively.Three laser beam are from different angle incident, and each laser beam can be adjusted flexibly at the focal position of fluid pool 41, and hot spot focusing quality is higher.The laser beam of wherein usining in the middle of being positioned at is as the lighting source of forward signal (FSC).
this light path device also comprises that one projects the first catoptron 31 on prism of corner cube for the fluorescence that each focal beam spot is inspired, and described in each, laser beam is from the one or both sides of the first catoptron 31 to fluid pool 41 incidents.
described fluid pool 41 is multiaspect column, specifically according to the incident angle of laser beam and quantity.The side of described fluid pool 41 comprise reflecting surface, and described reflecting surface over against exit facet, a plurality of planes of incidence between described reflecting surface and exit facet, described the first catoptron 31 is over against reflecting surface setting, described in each, laser beam is respectively from plane of incidence incident, and described prism of corner cube is over against exit facet setting.
in the present embodiment, described fluid pool 41 is octahedral column, the side of fluid pool 41 is comprised of a reflecting surface, exit facet over against reflecting surface, six planes of incidence laying respectively between reflecting surface and exit facet, and three laser beam are respectively from being positioned at three plane of incidence incidents of described first catoptron 31 the same sides.According to the face number of the incident angle of laser beam and quantity capable of regulating fluid pool 41 sides, the aperture of the fluid cavity of fluid pool 41 inside is less than 200um.
described prism of corner cube is Rhizoma Sparganii taper prism of corner cube 51.The summit of Rhizoma Sparganii taper prism of corner cube 51 is over against the exit facet setting of fluid pool 41, and has three sides towards fluid pool 41 and one bottom surface of fluid pool 41 dorsad.The fluorescence that focal beam spot inspires forms three fluorescence beam with different wave length after three side light splitting, and three fluorescence beam, respectively by after diaphragm 71 and collimating mirror 81, are received by fluorescence reception device.The setting position of diaphragm 71 is the projection focus of each fluorescence beam, and diaphragm 71, for the veiling glare of each fluorescence beam of filtering, improves signal to noise ratio (S/N ratio), and the size in diaphragm 71 holes is set according to the size of focus.Collimating mirror 81 is for collimating each fluorescence beam.The sidepiece of described prism of corner cube is also respectively arranged with one for adjusting the second catoptron 61 of the transmission direction of fluorescence beam.In the present embodiment three sides of Rhizoma Sparganii taper prism of corner cube 51 respectively correspondence arrange in one second catoptron 61(Fig. 2, corresponding the second catoptron 61 in side that is positioned at paper rear is not shown).From the fluorescence of Rhizoma Sparganii taper prism of corner cube 51 side projections, after the second catoptron 61 is adjusted transmission direction, at corresponding diaphragm 71, focus on, the second catoptron 61 can be adjusted the transmission direction of fluorescence light path flexibly, is convenient to the layout of light path.
described the first catoptron 31 is free-form surface mirror or non-spherical reflector, for the fluorescence that in fluid pool 41, each focal beam spot inspires is projeced into prism of corner cube, the fluorescence signal exciting of the corresponding some wavelength in each side of Rhizoma Sparganii taper prism of corner cube 51, the fluorescence of projection forms focus in certain position, and described diaphragm 71 is arranged on this focus place.
shown in Fig. 3, the bottom surface of Rhizoma Sparganii taper prism of corner cube 51 and the tiltangleθ between side can be according to the crevice projection angle adjustment of the separation degree of fluorescence beam and fluorescence beam, and general control is between 10 ~ 60 °.
embodiment bis-
shown in Fig. 4, the difference of the present embodiment and embodiment mono-is, described laser instrument 12 is six and is focused to six laser beam through six condenser lenses 22, six laser beam are respectively from being positioned at six plane of incidence incidents of described the first catoptron 32 both sides, and described prism of corner cube is hexagonal pyramid shape prism of corner cube 52.The summit of hexagonal pyramid shape prism of corner cube 52 is over against the exit facet setting of fluid pool 42, and has six sides towards fluid pool 41 and one bottom surface of fluid pool 42 dorsad.Fluorescence focal beam spot being inspired by the first catoptron 32 projects hexagonal pyramid shape prism of corner cube 52, forms six fluorescence beam with different wave length after six side light splitting.Six sides of hexagonal pyramid shape prism of corner cube 52 are provided with respectively one second catoptron 62 accordingly, six fluorescence beam are received by fluorescence reception device after adjusting the directions of propagation, diaphragm 72 filtering veiling glares, collimating mirror 82 collimations via the second catoptron 62 successively, and it is not shown in the drawings that wherein corresponding hexagonal pyramid shape prism of corner cube 52 is positioned at the second catoptron 62 that two sides at paper rear arrange, two diaphragms 72 corresponding thereto and collimating mirror 82.Other set-up mode is with embodiment mono-.
light path device of the present invention can be applicable on the fluid analysis equipment such as drain cell instrument, blood or urine.
above-described embodiment, for explanation technical conceive of the present invention and feature, is only a kind of preferred embodiment, and its object is that person skilled in the art can understand content of the present invention and implement according to this, can not limit protection scope of the present invention with this.Equivalent transformation or modification that all Spirit Essences according to the present invention are done, within all should being encompassed in protection scope of the present invention.
Claims (10)
1. the light path device of a fluid analysis equipment, comprise Ear Mucosa Treated by He Ne Laser Irradiation unit, fluid pool, fluorescence spectrophotometer unit, in described fluid pool, carry mobile fluid sample, the determinand that contains fluorochrome label in fluid sample, the fluid sample in described Ear Mucosa Treated by He Ne Laser Irradiation cellular convection body pond carries out Ear Mucosa Treated by He Ne Laser Irradiation, excite the fluorescence that obtains institute's mark, by being received by the fluorescence reception device of this fluid analysis equipment after the unit light splitting of described fluorescence spectrophotometer, it is characterized in that: described spectrophotometric unit comprises for described fluorescence being separated into the prism of corner cube of a plurality of fluorescence beam, described prism of corner cube is between fluid pool and the fluorescence reception device of this fluid analysis equipment.
2. light path device according to claim 1, it is characterized in that: described Ear Mucosa Treated by He Ne Laser Irradiation unit comprises a plurality of laser instruments, the laser beam that each laser instrument generates is focused on respectively the fluid sample place formation focal beam spot of fluid pool by least one condenser lens, the wavelength of the laser beam that a plurality of described laser instruments generate is different.
3. light path device according to claim 2, it is characterized in that: this light path device also comprises that one projects the first catoptron on prism of corner cube for the fluorescence that each focal beam spot is inspired, and described in each, laser beam is from the one or both sides of the first catoptron to fluid pool incident.
4. light path device according to claim 3, it is characterized in that: described fluid pool is multiaspect column, the side of described fluid pool comprise reflecting surface, and described reflecting surface over against exit facet, a plurality of planes of incidence between described reflecting surface and exit facet, described the first catoptron is over against reflecting surface setting, described in each, laser beam is respectively from plane of incidence incident, and described prism of corner cube is over against exit facet setting.
5. light path device according to claim 4, it is characterized in that: described fluid pool is octahedral column, described laser instrument is three, and three laser beam are respectively from being positioned at three plane of incidence incidents of described first catoptron the same side, and described prism of corner cube is Rhizoma Sparganii taper prism of corner cube.
6. light path device according to claim 4, it is characterized in that: described fluid pool is octahedral column, described laser instrument is six, and six laser beam are respectively from being positioned at six plane of incidence incidents of described the first catoptron both sides, and described prism of corner cube is hexagonal pyramid shape prism of corner cube.
7. light path device according to claim 3, is characterized in that: described the first catoptron is free-form surface mirror or non-spherical reflector.
8. light path device according to claim 1, is characterized in that: described light path device also comprises diaphragm, and described in each, fluorescence beam is received by fluorescence reception device after removing veiling glare by diaphragm respectively.
9. light path device according to claim 1, is characterized in that: described light path device also comprises collimating mirror, and described in each, fluorescence beam has fluorescence reception device to receive after collimating by collimating mirror respectively.
10. light path device according to claim 1, is characterized in that: described light path device also comprise be arranged at described prism of corner cube sidepiece for adjusting the second catoptron of the transmission direction of fluorescence beam.
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| CN201410355636.8A CN104155242B (en) | 2014-07-24 | 2014-07-24 | The light path device of fluid analysis apparatus |
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| CN201410355636.8A CN104155242B (en) | 2014-07-24 | 2014-07-24 | The light path device of fluid analysis apparatus |
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| CN104155242B CN104155242B (en) | 2016-08-17 |
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Cited By (5)
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| CN107525793A (en) * | 2017-10-17 | 2017-12-29 | 上海科源电子科技有限公司 | A kind of multichannel fluorescence detecting system |
| CN108414480A (en) * | 2018-01-26 | 2018-08-17 | 中国海洋石油集团有限公司 | A kind of crude oil fluorescence measuring device and method |
| CN110687034A (en) * | 2018-07-05 | 2020-01-14 | 深圳迈瑞生物医疗电子股份有限公司 | Laser irradiation system of flow cytometer and flow cytometer |
| CN114047170A (en) * | 2021-11-27 | 2022-02-15 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114047170A (en) * | 2021-11-27 | 2022-02-15 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114047170B (en) * | 2021-11-27 | 2022-08-16 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
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