CN206411125U - Portable fluorescence immunity analysis instrument - Google Patents
Portable fluorescence immunity analysis instrument Download PDFInfo
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- CN206411125U CN206411125U CN201621105735.1U CN201621105735U CN206411125U CN 206411125 U CN206411125 U CN 206411125U CN 201621105735 U CN201621105735 U CN 201621105735U CN 206411125 U CN206411125 U CN 206411125U
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Abstract
The utility model discloses a kind of Portable fluorescence immunity analysis instrument, including strip storehouse, for inserting fluorescent chromatographic quick diagnosis test strips；Excitation source, directly over the color development area of fluorescent chromatographic quick diagnosis test strips, for exciting the fluorescent material in fluorescent chromatographic quick diagnosis test strips, produces fluorescence emission；Camera is located at the acquisition channel upper inlet on excitation source, the fluorescence emission for gathering fluorescent test paper；Communicating circuit, the image for sending camera collection；Cpu chip, for controlling excitation source, camera and communicating circuit co-ordination.
Description
Technical field
The invention belongs to fluorescence immune chromatography field, more particularly to Portable fluorescence analytical technology.
Background technology
Fluorescence immune chromatography technology due to its sensitivity it is high, it is practical the features such as, can detection level it is very low such as
Protein, microorganism isoreactivity compound, thus be obtained in fields such as clinical treatment, food safety detections and widely should
With.The major way of fluorescence immune chromatography detection is to detect that the fluorescence of fluorescence immune chromatography test paper is strong by fluorescence immunity analyzer
Degree, so detector needs to be used together with Test paper in use, but fluorescence detector can be with general many detections
The fluorescence detection test of disparity items, such as either fluorescence cancer detection test paper or fluorescence early pregnancy Test paper, cooperation
All it is same fluorescence detector.
Accompanying drawing 1 is the schematic diagram that existing immunofluorescence chromatographs quick diagnosis test strips.Immunofluorescence chromatographs quick diagnosis
Test strips mainly specific antigen or antibody are coated on ribbon in the detection line of NC films, the antigen of fluorescence labeling or
Antibody adsorb on pad, after testing sample 101 is added on the well 105 of test strips one end, by capillarity to
The antigen or antibody of fluorescence labeling on preceding movement, dissolving pad, then it is moved to the detection line 106 of coated antigen or antibody
(T lines) place, if containing corresponding antibody or antigen, the antigen or antibody and fluorescent marker being coated in detection line in sample
With the corresponding antibodies in sample or antigen binding, immune complex is formed, fluorescent marker, which is enriched at detection line, forms one
The detection line for producing fluorescence 103 is excited by particular excitation light 102.If there is no corresponding antibodies, fluorescence labeling in serum to be checked
Thing will not will not be enriched with the antigen being coated in detection line or antibody binding, fluorescent marker, be not in detection line
The detection line of fluorescence can be produced.When sample and lysed fluorescent marker continue up to be moved to control line 104 (C lines),
Just with the specific antibody being coated at control line or antigen binding, immune complex is formed on control line, occurs one and leads to
Cross the control line for exciting and producing fluorescence.Detection line 106 and control line 104 are collectively forming color development area.
As shown in Figure 2, mainly by photodetector system, mechanism system swashs conventional fluorescent immunity analysis instrument
Luminescent system is constituted.Stepper motor 201 drive timing belt 202, the pallet 207 of strip is moved along a straight line, excite photosystem with
Transmitting photosystem is fixed on the fixed position above strip, and the effect of optical mirror slip group 204 is for distinguishing exciting light and hair
Light is penetrated, the front of LED excitation sources 205 has disposed narrow band pass filter, and it is only to allow the optical signal of excitation wavelength to pass through that it, which is acted on,.This
Sample can reduce fluorescence excitation and interference of the veiling glare to other materials as far as possible.Fluorescent material can produce glimmering after the light that is excited is excited
The transmitting light of optical signal, has disposed the narrow band pass filter of wavelength of transmitted light before photocell detection plate 203, and it is only to allow that it, which is acted on,
The optical signal of wavelength of transmitted light passes through, and can so mask exciting light and other material that may be present light that is excited is inspired
The veiling glare signal come.
As strip moves along a straight line, one kind can be formed in the conversion zone of strip by exciting photosystem to emit exciting light
Similar to the motion of scanning, during this motion, photocell detection plate 203 is entered to the transmitting optical signal received at any time
Row collection, because fluorescent material can produce the transmitting light of fluorescence signal after the light that is excited is excited, and fluorescence signal can be with glimmering
The concentration of stimulative substance is raised and strengthened, and the place without fluorescent material does not produce fluorescence signal then, so can by the above method
To detect fluorescent material is launched in strip fluorescence intensity.
Conventional fluorescent immunity analysis instrument has the following disadvantages：Mechanical moving structure has been used, in use can be due to
Mechanical part failure, such as timing belt loosen, and stepper motor loses step, and machine components are stuck to wait some problem to cause instrument to enter
Row detection or test position skew.The limitation of examined circuit AD conversion speed and mechanical moving structure translational speed, is completed whole
The individual detection most short time for being also required to 30 seconds or so.Due to being controlled by stepper motor to the movement of pallet, when strip due to
The small change of size that batch reason is caused can cause pallet during insertion to exceed the locking of stepper motor to the resistance of strip
Strength, causes strip not to be inserted into position in pallet, and then influence testing result.Due to mechanical structure and the space of linear motion
Cause equipment instrument excessive, it is impossible to realize miniaturization and portability.Due to used stepper motor and in detection process each
Part will cause whole equipment power higher, civil power must be connected in use with mechanical part co-ordination, it is impossible to
Realize the use in the environment such as portable or field, open air.Presence and the position of corresponding supporting machinery structure due to mechanical structure
The detection of electrons put, also makes the cost increase of whole instrument very big.It is unfavorable for the popularization and application of equipment.
The content of the invention
(1) technical problem to be solved
The technical problem to be solved in the present invention be solve conventional fluorescent immunity analysis instrument easily influenceed by mechanical breakdown, it is inconvenient
Take, there is provided the Portable fluorescence immunity analysis instrument of a kind of convenience, the high and low cost of accuracy rate the problems such as cost is high.
(2) technical scheme
In order to solve the above-mentioned technical problem, the invention provides a kind of Portable fluorescence analyzer, including strip storehouse, it is used for
Insert fluorescent chromatographic quick diagnosis test strips；Excitation source, positioned at fluorescent chromatographic quick diagnosis test strips color development area just on
Side, for exciting the fluorescent material in fluorescent chromatographic quick diagnosis test strips, produces fluorescence emission；Camera is located at exciting light
At acquisition channel upper inlet on source, the fluorescence emission for gathering fluorescent test paper；Communicating circuit, for sending camera
The image of collection；Cpu chip, for controlling excitation source, camera and communicating circuit co-ordination.
It is preferred that, the excitation source is four particular excitation light waves being symmetrically distributed on four angles of exciting light source plate
Long lamp.Optionally, the excitation source is the lamp of 2 or more than 2 symmetrical particular excitation optical wavelength.
It is preferred that, there is the optical filter of particular excitation optical wavelength in front of the excitation source (302).
It is preferred that, there is the optical filter of fluorescent emission optical band in front of camera (301) head.
It is preferred that, identification code can be added around the color development area of the fluorescent chromatographic quick diagnosis test strips, for area
Divide the type and batch information of test paper.
Optionally, the cpu chip can be analyzed the image of shooting, obtain the light intensity of corresponding transmitting optical band.
Optionally, the communicating circuit can be WIFI, bluetooth, infrared or usb modules.
Present invention also offers a kind of Portable fluorescence analysis system, including：Portable fluorescence analyzer, for fluorescence
Chromatography quick diagnosis test strips are taken pictures；Computer, for controlling Portable fluorescence immunity analysis instrument to examination by communicating circuit
Paper is taken pictures, and receives the picture of Portable fluorescence analyzer transmission, and the test paper image collected is analyzed；Wherein,
The Portable fluorescence analyzer includes：Strip storehouse, for inserting fluorescent chromatographic quick diagnosis test strips；Excitation source, positioned at glimmering
Directly over the color development area of photosphere analysis quick diagnosis test strips, for exciting the fluorescence in fluorescent chromatographic quick diagnosis test strips
Matter, produces fluorescence emission；Camera is located at the acquisition channel upper inlet on excitation source, for gathering fluorescent test paper
Fluorescence emission；Communicating circuit, the image for sending camera collection；Cpu chip, for controlling excitation source, camera
And communicating circuit co-ordination.
(3) beneficial effect
By the Portable fluorescence analyzer and Portable fluorescence analysis system of the present invention, fluorescence analyser can be made
Very compact portable part, so as to anywhere be rapidly performed by pattern detection.
Brief description of the drawings
Fig. 1 is the schematic diagram that existing immunofluorescence chromatographs quick diagnosis test strips.
Fig. 2 is traditional fluorescence immunity analyzer schematic diagram.
Fig. 3 is the Portable fluorescence immunity analysis instrument schematic diagram of the present invention.
Fig. 4 is the partial structural diagram of the excitation source part of the present invention.
Fig. 5 is the partial structurtes top view that the excitation source of the present invention is the excitation source part of two.
Fig. 6 is the partial structural diagram of the strip storehouse part of the present invention.
Fig. 7 is the partial structural diagram after strip storehouse part of the invention is taken apart.
Fig. 8 is that the immunofluorescence of the present invention chromatographs the schematic diagram of quick diagnosis test strips.
Fig. 9 is the system structure diagram of the fluorescence immunity analyzer of the present invention.
Embodiment
Refer to the attached drawing 3, Portable fluorescence immunity analysis instrument of the invention includes camera 301, excitation source 302, strip
Storehouse 303.It can also include cpu chip and communicating circuit inside the portable immunity analysis instrument of the present invention.Cpu chip and communication electricity
Road can be located at the inside optional position of the portable immunity analysis instrument of the present invention, and concrete structure is referring to Fig. 9.
Strip storehouse 303 is used to insert strip 304, and excitation source 302 is located at the surface of the color development area of strip 304, excited
There is the optical filter of particular excitation optical wavelength in the front of light source 302, for example, it may be wavelength for 365nm optical filter or its
The optical filter of all band.Exciting light after optical filter is filtered is by exciting optical channel 305 can be with vertical irradiation in strip 304
Color development area on.Fluorescent material on test paper produces fluorescence emission under the irradiation of excitation source.
Camera 301 is located at the upper inlet of acquisition channel 306 on excitation source 302, and it includes the knot such as CCD, CMOS
Structure, the fluorescent emission luminous intensity for gathering fluorescent test paper.There is the optical filtering of individual fluorescent emission optical band in the front of camera 301
Piece, if for example, strip can produce 615nm transmitting light under 365nm excitation, before the camera lens of camera 301
The camera lens for entering camera after 615nm optical filter, the optical filter that transmitting light passes through 615nm is placed, 615nm optical signal is shot
As head is collected.Cpu chip can be analyzed the image of shooting, obtain the light intensity of corresponding transmitting optical band.Communicating circuit
Picture is directly sent to the computer being associated, optionally, the light intensity that can also obtain analysis sends what is be associated to
Computer.
Fig. 4 is the partial structural diagram of the excitation source 302 of the present invention.The excitation source can be LED, laser,
Bulb, fluorescent tube etc. are any can to send the lamp of particular excitation optical wavelength.The present invention is done with the lamp 401 with particular excitation optical wavelength
For light source, the narrow band pass filter (not identifying) of a particular excitation optical wavelength is placed before LED.As illustrated, one has 4
Individual excitation source lamp 401a-401d is symmetrically distributed in four angles of exciting light source plate, and the center of excitation source has acquisition channel
306 entrance 402.Optionally, excitation source can be 2 symmetrical excitation source lamps, as shown in figure 5, excitation source
Lamp 501a and 501b are symmetrically distributed in the both sides of exciting light source plate.The center of excitation source has the entrance of acquisition channel 306
502.Optionally, excitation source can be the lamp of more particular excitation optical wavelength, as long as being distributed symmetrically.In addition, swashing
The quantity of light emitting source can also be any number, and the light source of such as 1 oblique upper can also meet the requirement of exciting light.
Fig. 6 is the partial structural diagram of the strip storehouse part of the present invention.Strip 304 is inserted in strip storehouse 303.As schemed
Shown in 7, after the insertion strip of strip 304 storehouse 303, color development area 304a, which is just aligned, excites optical channel 305.
Strip 304 can be using existing immunofluorescence chromatography quick diagnosis test strips as shown in Figure 1.Furthermore it is possible to
The identification code of bar code or Quick Response Code or other any coded systems is added around the color development area of strip, for distinguishing test paper
Type and batch information, be as shown in Figure 8, in dotted line frame 802 color development area.C lines represent control line, and T lines represent test
The concentration of material to be checked in line, detection T lines fluorescent values reaction sample, the height of T line fluorescent values and the concentration of material to be checked into than
The concentration of thing to be checked is higher in example relation, the higher representative sample of fluorescent value.Extraneous ambient light has many differences, but T lines
With C lines it is under same ambient light, and the fluorescent value that C lines are represented is standard fluorescence value, and C lines represent check plot,
So as to can accurately draw the fluorescent value of T lines as reference using C lines.Well S is used to place tested sample.Optionally, some
Test paper can also not have well S, can be inhale lining bar or can also by sample directly as colour developing area.Identification code 801 can
Be Quick Response Code, bar code or other be used for recognize coded system, type and batch information for distinguishing test paper, identification code
The optional position on test strips and shell can be located at.In addition, optional, Quick Response Code may reside in strip, in packaging bag,
On packing box, or in some annex.It can be located above colour developing bar as shown in Figure 8, but be not limited thereto, people in the art
Member is it is appreciated that identification code can also be located at below colour developing bar or side optional position, as long as causing near color development area
Camera can collect its image.
The present invention system architecture as shown in figure 9, the present invention Portable fluorescence immunity analysis instrument 901 include cpu chip,
For controlling excitation source, camera and communicating circuit co-ordination, optionally, camera can also be clapped in cpu chip
The image taken the photograph is analyzed, and obtains the fluorescent value light intensity of corresponding transmitting optical band.Communicating circuit can be WIFI, bluetooth, infrared
Or usb modules.Computer 902 can be desk-top or Portable tablet personal computer, or the mobile terminal with intelligent function.In step
S1, computer 902 controls Portable fluorescence immunity analysis instrument to take pictures test paper by communicating circuit.In step S2, camera
The picture of shooting is transmitted back to computer 902 by the communicating circuit of Portable fluorescence immunity analysis instrument 901.When test paper picture includes
During identification code, the program on step S3, computer can analyze the bar code included in the image collected or Quick Response Code etc.
Information of identification code, then sends information of identification code to server 903.In step S4, server 903 is believed according to the identification code of reception
Corresponding batch information in breath inquiry database, and send corresponding batch information to computer 902.At the same time, computer is obtained
The fluorescent value of the test paper reflected into image, and calculated according to the batch information of acquisition, obtain the inspection that test paper is reflected
Survey result.
When test paper is the common immunofluorescence test paper not comprising identification code, in step S2, computer 902 can be directly to adopting
The test paper image collected is analyzed, and gets the fluorescent value of the test paper reflected in image, is believed according to built-in acquiescence batch
Breath, or batch information is manually entered, calculated, obtain the testing result that test paper is reflected.According to fluorescent value, batch information
The method for calculating testing result can be any one of existing fluorescence immune analysis method.If immune point of Portable fluorescence
The cpu chip of analyzer has calculated the fluorescent value of image reflection, then computer directly calculates testing result using the fluorescent value.
By the method and system of the present invention, user can it is simple by the Portable fluorescence immunity analysis instrument of the present invention and
Test sample is accurately carried out, the limitation of the mechanical moving structure of existing fluorescence immunity analyzer, specific fluorescence has been broken away from
Value computing is realized by computer or mobile terminal so that fluorescence immunity analyzer of the invention is compact portable, can be used in
In any environment.Bar code or 2 D code information while gathering fluorescence signal using camera on collection test paper, it is so same
Traditional instrument saves a set of reading bar code or the mechanism of Quick Response Code compared to us, is decreased while cost is reduced
The operating procedure and conventional machines of operating personnel is needed required for first gathering bar code or Quick Response Code and then gathering fluorescence signal again
Time.
Particular embodiments described above, has been carried out further in detail to the purpose of the present invention, technical scheme and beneficial effect
Describe in detail bright, it should be understood that the foregoing is only the present invention specific embodiment, be not intended to limit the invention, it is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements done etc. should be included in the protection of the present invention
Within the scope of.
Claims (18)
1. a kind of Portable fluorescence immunity analysis instrument, including：
Strip storehouse (303), for inserting fluorescent chromatographic quick diagnosis test strips；
Excitation source (302), directly over the color development area of fluorescent chromatographic quick diagnosis test strips, for exciting fluorescent chromatographic
Fluorescent material in quick diagnosis test strips, produces fluorescence emission；
Camera (301) is located at acquisition channel (306) upper inlet on excitation source (302), for gathering fluorescent test paper
Fluorescence emission；
Communicating circuit, the image for sending camera collection；
Cpu chip, for controlling excitation source, camera and communicating circuit co-ordination.
2. Portable fluorescence immunity analysis instrument as claimed in claim 1, wherein：
The excitation source (302) is the lamp of four symmetrical particular excitation optical wavelength.
3. Portable fluorescence immunity analysis instrument as claimed in claim 1 or 2, wherein：
There is the optical filter of particular excitation optical wavelength in front of the excitation source (302).
4. Portable fluorescence immunity analysis instrument as claimed in claim 1, wherein：
The excitation source (302) is the lamp of 2 or more than 2 symmetrical particular excitation optical wavelength.
5. Portable fluorescence immunity analysis instrument as claimed in claim 1, wherein：
There is the optical filter of fluorescent emission optical band in front of the camera (301).
6. Portable fluorescence immunity analysis instrument as claimed in claim 1, wherein：
Identification code, the type for distinguishing test paper can be included around the color development area of the fluorescent chromatographic quick diagnosis test strips
And batch information.
7. Portable fluorescence immunity analysis instrument as claimed in claim 1, wherein：
The cpu chip can be analyzed the image of shooting, obtain the light intensity of corresponding transmitting optical band.
8. Portable fluorescence immunity analysis instrument as claimed in claim 1, wherein：
The communicating circuit can be WIFI, bluetooth, infrared or usb modules.
9. a kind of Portable fluorescence analysis system, including：
Portable fluorescence immunity analysis instrument, for being taken pictures to fluorescent chromatographic quick diagnosis test strips；
Computer, for controlling Portable fluorescence immunity analysis instrument to take pictures test paper by communicating circuit, and receives portable
The picture that fluorescence immunity analyzer is sent, is analyzed the test paper image collected；
Wherein, the Portable fluorescence immunity analysis instrument includes：
Strip storehouse (303), for inserting fluorescent chromatographic quick diagnosis test strips；
Excitation source (302), directly over the color development area of fluorescent chromatographic quick diagnosis test strips, for exciting fluorescent chromatographic
Fluorescent material in quick diagnosis test strips, produces fluorescence emission；
Camera (301) is located at acquisition channel (306) upper inlet on excitation source (302), for gathering fluorescent test paper
Fluorescence emission；
Communicating circuit, the image for sending camera collection；
Cpu chip, for controlling excitation source, camera and communicating circuit co-ordination.
10. Portable fluorescence analysis system as claimed in claim 9, further comprises：
Server, the information of identification code for receiving computer transmission inquires about correspondence in database according to the information of identification code of reception
Batch information, and send corresponding batch information to computer.
11. Portable fluorescence analysis system as claimed in claim 9, wherein
The computer can be desk-top or Portable tablet personal computer, or the mobile terminal with intelligent function.
12. Portable fluorescence analysis system as claimed in claim 9, wherein：
The excitation source (302) is four particular excitation optical wavelength being symmetrically distributed on four angles of exciting light source plate
Lamp.
13. the Portable fluorescence analysis system as described in claim 9 or 12, wherein：
There is the optical filter of particular excitation optical wavelength in front of the excitation source (302).
14. Portable fluorescence analysis system as claimed in claim 9, wherein：
The excitation source (302) is the lamp of 2 or more than 2 symmetrical particular excitation optical wavelength.
15. Portable fluorescence analysis system as claimed in claim 9, wherein：
There is the optical filter of fluorescent emission optical band in front of the camera (301).
16. Portable fluorescence analysis system as claimed in claim 9, wherein：
Identification code, the type for distinguishing test paper can be included around the color development area of the fluorescent chromatographic quick diagnosis test strips
And batch information.
17. Portable fluorescence analysis system as claimed in claim 9, wherein：
The cpu chip can be analyzed the image of shooting, obtain the light intensity of corresponding transmitting optical band.
18. Portable fluorescence analysis system as claimed in claim 9, wherein：
The communicating circuit can be WIFI, bluetooth, infrared or usb modules.
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| CN201621105735.1U CN206411125U (en) | 2016-09-30 | 2016-09-30 | Portable fluorescence immunity analysis instrument |
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| CN201621105735.1U CN206411125U (en) | 2016-09-30 | 2016-09-30 | Portable fluorescence immunity analysis instrument |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107884571A (en) * | 2016-09-30 | 2018-04-06 | 张磊 | Portable fluorescence immunity analysis instrument and Portable fluorescence analysis method |
| CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
| CN113039429A (en) * | 2018-11-14 | 2021-06-25 | 韩国巴迪泰生物科技有限公司 | Integrated immunodiagnostic fluorescence reader with multiple diagnostic functions |
-
2016
- 2016-09-30 CN CN201621105735.1U patent/CN206411125U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107884571A (en) * | 2016-09-30 | 2018-04-06 | 张磊 | Portable fluorescence immunity analysis instrument and Portable fluorescence analysis method |
| CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
| CN113039429A (en) * | 2018-11-14 | 2021-06-25 | 韩国巴迪泰生物科技有限公司 | Integrated immunodiagnostic fluorescence reader with multiple diagnostic functions |
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Effective date of registration: 20180627 Address after: 225300 Taizhou, Jiangsu, China, west side of Tai Chi Road, China's medicine, building G59, 63 to three, west of Lu Jia Lu. Patentee after: JIANGSU DAJUN BIOTECHNOLOGY CO., LTD. Address before: 300134 Tianjin Beichen District Chen Xing Road, Longyang Road, Rukang home 5-403 Co-patentee before: JIANGSU DAJUN BIOTECHNOLOGY CO., LTD. Patentee before: Zhang Lei Co-patentee before: Nanjing hundred measuring biological science and Technology Co Ltd |
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Effective date of registration: 20200602 Address after: 225300 Jiangsu city of Taizhou province China Road West of Lu Jia Lu, Chengkou pharmaceutical G59 East Building 63, one to three layers of the West Co-patentee after: Kunming lianen Biotechnology Co.,Ltd. Patentee after: JIANGSU DAJUN BIOTECHNOLOGY Co.,Ltd. Co-patentee after: Zhang Lei Address before: 225300 Jiangsu city of Taizhou province China Road West of Lu Jia Lu, Chengkou pharmaceutical G59 East Building 63, one to three layers of the West Patentee before: JIANGSU DAJUN BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20210903 Address after: Room 703-01, 7 / F, building 1, No. A5, Rongchang East Street, Beijing Economic and Technological Development Zone, Daxing District, Beijing 100101 Patentee after: BEIJING ANBAISHENG DIAGNOSIS TECHNOLOGY Co.,Ltd. Patentee after: Kunming lianen Biotechnology Co.,Ltd. Patentee after: Zhang Lei Address before: 225300 Taizhou, Jiangsu, China, west side of Tai Chi Road, China's medicine, building G59, 63 to three, west of Lu Jia Lu. Patentee before: JIANGSU DAJUN BIOTECHNOLOGY Co.,Ltd. Patentee before: Kunming lianen Biotechnology Co.,Ltd. Patentee before: Zhang Lei |