CN104880450A - Line focusing detection system for immunofluorescence reagent card - Google Patents
Line focusing detection system for immunofluorescence reagent card Download PDFInfo
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- CN104880450A CN104880450A CN201510339614.7A CN201510339614A CN104880450A CN 104880450 A CN104880450 A CN 104880450A CN 201510339614 A CN201510339614 A CN 201510339614A CN 104880450 A CN104880450 A CN 104880450A
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Abstract
The invention discloses a line focusing detection system for an immunofluorescence reagent card. The line focusing detection system comprises a light-emitting light source, a first light filter, a first convex lens, a spectroscope, a plano-convex cylindrical lens, a second convex lens, a second light filter and a PCB (Printed Circuit Board) arranged in sequence, wherein the light-emitting light source is a point light source for emitting parallel light; light beams pass through the first light filter to eliminate the interference light; the plano-convex cylindrical lens is located below the spectroscope and converges reflected light to form a linear excitation light spot, the linear excitation light spot irradiates a detected object under the plano-convex cylindrical lens, the detected object is excited by the linear excitation light spot to emit fluorescence, and the fluorescence is returned to the spectroscope through the plano-convex cylindrical lens; the second convex lens is located above the spectroscope; the converged light passing through the second convex lens reaches a photodiode above the second light filter through the second light filter; the PCB is used for receiving and analyzing an electric signal from the photodiode; the line focusing detection system further comprises a guide rail; the light-emitting light source and the PCB are mounted on the guide rail. The line focusing detection system can improve the fluorescence emission intensity, improve the fluorescence measurement signal intensity of immunofluorescence reagent, and greatly improve the detection sensitivity.
Description
Technical field
The invention belongs to the technical field of optical detecting channel design, particularly relate to a kind of line focus detection system and method for immunofluorescent reagent card.
Background technology
At present, immunochromatography (lateral flow immunoassay, LFIA) Fast Detection Technique is based upon technology on chromatographic technique and Ag-Ab specific immune response basis.Fluorescence immune chromatography test paper bar test strips, using fluorchrome as label, can be widely used in situ quantitation and detect, and is the important directions of following instant detection technique development.
Immunofluorescent reagent card detects the concentration of certain material, and detected material is exactly subject matter, and subject matter meeting and fluorchrome combine, and after accepting laser irradiation, meeting emitting fluorescence, subject matter concentration is high, and emitting fluorescence intensity is large.The detailed process that fluorescence immune chromatography test paper bar detects is that fluorochromes process is carried out in detection line (T) and nature controlling line (C) region in test strips.Test strips is put into detection sample, when detecting in sample the examination target thing containing energy and fluorchrome combination, T line and C line region will send the fluorescence of varying strength.The course of work of immuno-chromatographic test paper strip quantitative detecting analysis instrument is that test strips is inserted deck, then linear stepping motor scanning test strips is driven, exciting light is launched in scanning process, receive specific wavelength emitting fluorescence, obtain the fluorescence intensity of test strips CT line, thus analyze the information such as the concentration measuring subject matter.
In Immunofluorescence test paper strip quantitative detecting analysis, the part of core is the design of optical detecting channel.Current excitation source has varied.Light emitting diode of the prior art is neither pointolite, and also non-collimated light sources, can not meet the requirement of high precision test, there is the problems such as immunofluorescent reagent card fluorescence excitation beam intensity ratio is more weak, launching efficiency is not high.
Summary of the invention
For overcoming the deficiencies in the prior art, the object of the present invention is to provide a kind of line focus detection system of immunofluorescent reagent card, be intended to improve the launching efficiency of parallel light source for fluorescent material on immunofluorescent reagent card upper control line and detection line, strengthen fluorescence excitation light intensity, thus improve the precision of optical detection.
For achieving the above object, the present invention is achieved through the following technical solutions:
The line focus detection system of immunofluorescent reagent card, comprises and placing successively:
Illuminating source 1, it is the pointolite of emitting parallel light;
First optical filter 2, described parallel pointolite light beam passes through described first optical filter to eliminate stray light;
First convex lens 3, it is for assembling the light beam by the first optical filter;
Spectroscope 5, it is for being divided into a branch of reflected light and through Beam by converging light;
Also comprise: be positioned at the plano-convex cylindrical lens 4 below described spectroscope, it is got described reflected light and is converged on the detected material 10 that to be radiated at after wire excites hot spot and to be positioned at below it, described detected material excites hot spot excitation-emission to go out fluorescence by described wire, and described fluorescence returns described spectroscope again via described plano-convex cylindrical lens;
Second convex lens 6, it is positioned at above described spectroscope, gets and assembles through described second convex lens through described spectroscopical transmitted light;
Second optical filter 7, it is positioned at the top of described second convex lens, and arrive photodiode 8 above it by the converging light of described second convex lens through the second optical filter, the light signal of convergence is converted to electric signal by described photodiode;
PCB 9, it is positioned at above described illuminating source and photodiode, for receiving and analyze the electric signal from photodiode;
Wherein, also comprise a guide rail, described illuminating source and PCB can the mode of straight line interlock movement be arranged on described guide rail, the opening time of described PCB control illuminating source.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, described spectroscope is arranged in a movable spherical structure, regulate spectroscopical direction by the rotation of spheroid, thus regulate the direction of incident beam, make incident light vertical irradiation detected material.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, described detected material is immunofluorescent reagent card.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, described immunofluorescent reagent card comprises control line 11 and detection line 12.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, described plano-convex cylindrical lens position is specially and makes described wire excite hot spot to be radiated on described detection line.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, described PCB comprises 51 single-chip microcomputers.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, also comprises leading screw, and above-mentioned all parts are all be connected by described leading screw.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, also comprises motor, and it is for controlling described leading screw.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, described PCB controls motor and walks about.
Preferably, the line focus detection system of described immunofluorescent reagent card, wherein, described detection line is many detection lines, and described wire excites hot spot by Electric Machine Control to travel through described many detection lines.
Beneficial effect of the present invention: in Immunofluorescence test paper strip quantitative test, need suitable pointolite or directional light is focused on, such fluorescence excitation efficiency can be high, the present invention devises a kind of detection system of Immunofluorescence test paper strip mainly for light-resource fousing, greatly increase the scope of light source activation fluorescent material, thus strengthen fluorescence excitation intensity, improve the accuracy of detection of immunofluorescence quantitative reagent card, be embodied in the following aspects:
(1) for the profile of Immunofluorescence test paper strip detection line, adopt the pointolite of emitting parallel light, the focusing effect of light own is better.
(2) convergent beam changes beam direction by spectroscope, makes utilizing emitted light can the fluorescent material be irradiated on immunofluorescent reagent card on detection line and nature controlling line of maximum magnitude, greatly can improve launching efficiency and the luminous intensity of fluorescence.
(3) have employed plano-convex cylindrical lens, be converged to wire excite hot spot by being changed the reflected light after light path by spectroscope, be then radiated on the detected material that is positioned at below it, detected material excites hot spot excitation-emission to go out fluorescence by wire; Wire excites hot spot can improve the launching efficiency for fluorescent material on immunofluorescent reagent card upper control line and detection line of parallel light source, strengthens fluorescence excitation light intensity, thus improves the precision of optical detection.
(4) checking matter is under exciting light irradiates, emitting fluorescence has diversity, again be irradiated to spectroscope through plano-convex cylindrical lens, the most fluorescence of spectroscope transmission, reflection interference light simultaneously, through spectroscopical fluorescence because the reasons such as refraction can produce certain Divergent Phenomenon, get the convergence of transmitted light through the second convex lens, substantially increase the detection efficiency of fluorescence.
(5) first optical filters and the second optical filter, eliminate stray light further, improves the fluorescence precision arriving photodiode.Whole light path design drastically increases radiative launching efficiency, meanwhile, improve the precision of fluoroscopic examination, the present invention is mainly used in fluorescence immune chromatography test paper bar quantitative detecting analysis instrument, be applied in actual instrumentation production, Detection results is improved significantly.
Accompanying drawing explanation
Detection system schematic diagram in the line focus detection system that Fig. 1 is the immunofluorescent reagent card described in one embodiment of the invention;
Immunofluorescent reagent card structure schematic diagram in the line focus detection system that Fig. 2 is the immunofluorescent reagent card described in one embodiment of the invention;
Spectroscope spherical structure in the line focus detection system that Fig. 3 is the immunofluorescent reagent card described in one embodiment of the invention;
Plano-convex cylindrical lens Path of Convergent Rays in the line focus detection system that Fig. 4 is the immunofluorescent reagent card described in one embodiment of the invention.
Wherein, 1-illuminating source, 2-first optical filter, 3-first convex lens, 4-plano-convex cylindrical lens, 5-spectroscope, 6-second convex lens, 7-second optical filter, 8-photodiode, 9-PCB circuit board, 10-detected material, 11-control line, 12-detection line.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to instructions word to make those skilled in the art.
A line focus detection system for immunofluorescent reagent card, increases the scope of light source activation fluorescent material greatly, thus strengthens fluorescence excitation intensity, improve the accuracy of detection of immunofluorescence quantitative reagent card, refer to accompanying drawing 1, comprises straight line is placed successively:
Illuminating source 1, it is the pointolite of emitting parallel light; For the profile of Immunofluorescence test paper strip detection line, adopt the pointolite of emitting parallel light, the focusing effect of light own is better.
First optical filter 2, described parallel pointolite light beam passes through described first optical filter to eliminate stray light;
First convex lens 3, it is for assembling the light beam by the first optical filter;
Spectroscope 5, it is for being divided into a branch of reflected light and through Beam by converging light; Convergent beam changes beam direction by spectroscope, makes utilizing emitted light can the fluorescent material be irradiated on immunofluorescent reagent card on detection line and nature controlling line of maximum magnitude, greatly can improve launching efficiency and the luminous intensity of fluorescence;
Also comprise: be positioned at the plano-convex cylindrical lens 4 below described spectroscope, it is got described reflected light and is converged on the detected material 10 that to be radiated at after wire excites hot spot and to be positioned at below it, described detected material excites hot spot excitation-emission to go out fluorescence by described wire, wire excites hot spot can improve the launching efficiency for fluorescent material on immunofluorescent reagent card upper control line and detection line of parallel light source, strengthen fluorescence excitation light intensity, thus improve the precision of optical detection; The Path of Convergent Rays of plano-convex cylindrical lens refers to accompanying drawing 4;
Described fluorescence returns described spectroscope again via described plano-convex cylindrical lens; Checking matter is under exciting light irradiates, emitting fluorescence has diversity, again be irradiated to spectroscope through plano-convex cylindrical lens, the most fluorescence of spectroscope transmission, reflection interference light simultaneously, through spectroscopical fluorescence because the reasons such as refraction can produce certain Divergent Phenomenon, get the convergence of transmitted light through the second convex lens, substantially increase the detection efficiency of fluorescence; Second convex lens 6, it is positioned at above described spectroscope, gets and assembles through described second convex lens through described spectroscopical transmitted light;
Second optical filter 7, it is positioned at the top of described second convex lens, and arrive photodiode 8 above it by the converging light of described second convex lens through the second optical filter, the light signal of convergence is converted to electric signal by described photodiode; First optical filter and the second optical filter, eliminate stray light further, improves the fluorescence precision arriving photodiode;
PCB 9, it is positioned at above described illuminating source and photodiode, for receiving and analyze the electric signal from photodiode;
Wherein, also comprise a guide rail, described illuminating source and PCB can the mode of straight line interlock movement be arranged on described guide rail, the opening time of described PCB control illuminating source.Whole light path design drastically increases radiative launching efficiency, meanwhile, improve the precision of fluoroscopic examination, the present invention is mainly used in fluorescence immune chromatography test paper bar quantitative detecting analysis instrument, be applied in actual instrumentation production, Detection results is improved significantly.
Further, refer to accompanying drawing 3, described spectroscope is arranged in a movable spherical structure, regulates spectroscopical direction by the rotation of spheroid, thus regulates the direction of incident beam, makes incident light vertical irradiation detected material.
Further, described detected material is immunofluorescent reagent card.
Further, refer to accompanying drawing 2, described immunofluorescent reagent card comprises control line 11 and detection line 12.
Further, described plano-convex cylindrical lens position is specially and makes described wire excite hot spot to be radiated on described detection line.
Further, described PCB comprises 51 single-chip microcomputers.
Further, also comprise leading screw, above-mentioned all parts are all be connected by described leading screw.
Further, also comprise motor, it is for controlling described leading screw.
Further, described PCB control motor is walked about.
Further, described detection line is many detection lines, and described wire excites hot spot by Electric Machine Control to travel through described many detection lines, wherein many refer to two and more than.
Although embodiment of the present invention are open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (10)
1. a line focus detection system for immunofluorescent reagent card, is characterized in that, comprises placing successively:
Illuminating source (1), it is the pointolite of emitting parallel light;
First optical filter (2), described parallel pointolite light beam passes through described first optical filter to eliminate stray light;
First convex lens (3), it is for assembling the light beam by the first optical filter;
Spectroscope (5), it is for being divided into a branch of reflected light and through Beam by converging light;
Also comprise: be positioned at the plano-convex cylindrical lens (4) below described spectroscope, it is got described reflected light and is converged on the detected material (10) that to be radiated at after wire excites hot spot and to be positioned at below it, described detected material excites hot spot excitation-emission to go out fluorescence by described wire, and described fluorescence returns described spectroscope again via described plano-convex cylindrical lens;
Second convex lens (6), it is positioned at above described spectroscope, gets and assembles through described second convex lens through described spectroscopical transmitted light;
Second optical filter (7), it is positioned at the top of described second convex lens, and arrive photodiode (8) above it by the converging light of described second convex lens through the second optical filter, the light signal of convergence is converted to electric signal by described photodiode;
PCB (9), it is positioned at above described illuminating source and photodiode, for receiving and analyze the electric signal from photodiode;
Wherein, also comprise a guide rail, described illuminating source and PCB can the mode of straight line interlock movement be arranged on described guide rail, the opening time of described PCB control illuminating source.
2. the line focus detection system of immunofluorescent reagent card as claimed in claim 1, it is characterized in that, described spectroscope is arranged in a movable spherical structure, spectroscopical direction is regulated by the rotation of spheroid, thus regulate the direction of incident beam, make incident light vertical irradiation detected material.
3. the line focus detection system of immunofluorescent reagent card as claimed in claim 1, it is characterized in that, described detected material is immunofluorescent reagent card.
4. the line focus detection system of immunofluorescent reagent card as claimed in claim 3, it is characterized in that, described immunofluorescent reagent card comprises control line 11 and detection line 12.
5. the line focus detection system of immunofluorescent reagent card as claimed in claim 4, it is characterized in that, described plano-convex cylindrical lens position is specially and makes described wire excite hot spot to be radiated on described detection line.
6. the line focus detection system of immunofluorescent reagent card as claimed in claim 1, it is characterized in that, described PCB comprises 51 single-chip microcomputers.
7. the line focus detection system of immunofluorescent reagent card as claimed in claim 1, it is characterized in that, also comprise leading screw, above-mentioned all parts are all be connected by described leading screw.
8. the line focus detection system of immunofluorescent reagent card as claimed in claim 7, it is characterized in that, also comprise motor, it is for controlling described leading screw.
9. the line focus detection system of immunofluorescent reagent card as claimed in claim 8, it is characterized in that, described PCB controls described motor and walks about.
10. the line focus detection system of the immunofluorescent reagent card as described in claim 5 or 9, is characterized in that, wherein said detection line is many detection lines, and described wire excites hot spot by described Electric Machine Control to travel through described many detection lines.
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| CN201510339614.7A CN104880450A (en) | 2015-06-18 | 2015-06-18 | Line focusing detection system for immunofluorescence reagent card |
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| CN201510339614.7A CN104880450A (en) | 2015-06-18 | 2015-06-18 | Line focusing detection system for immunofluorescence reagent card |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105242039A (en) * | 2015-10-29 | 2016-01-13 | 詹爱军 | Fluorescent immunochromatography quantitative analyzer and method |
| CN106353289A (en) * | 2016-09-19 | 2017-01-25 | 苏州微析生物科技有限公司 | Light path system of POCT fluorescent quantitative analyser and fluorescent quantitative analysis method |
| CN107219631A (en) * | 2017-06-09 | 2017-09-29 | 北京百康芯生物科技有限公司 | A kind of utilization LED/light source produces the device of uniform strip light spots |
| CN107561042A (en) * | 2016-07-01 | 2018-01-09 | 杭州凯珥医疗科技有限公司 | A kind of spot shaping optical system for fluorescence analyser |
| WO2018058525A1 (en) * | 2016-09-30 | 2018-04-05 | 深圳市检验检疫科学研究院 | Fluorescence immunochromatography quantitative analysis instrument and method |
| CN109632741A (en) * | 2018-12-27 | 2019-04-16 | 苏州和迈精密仪器有限公司 | A kind of detection method of composite fluorescence microballoon reagent card |
| CN110806399A (en) * | 2019-08-19 | 2020-02-18 | 湖南德荣生物医学工程有限公司 | Portable fluorescence immunoassay quantitative analyzer |
| CN111624331A (en) * | 2020-05-22 | 2020-09-04 | 军事科学院系统工程研究院卫勤保障技术研究所 | Multichannel synchronous fluorescence immunochromatography detection system |
| CN113933496A (en) * | 2021-07-19 | 2022-01-14 | 安徽桐康医疗科技股份有限公司 | A calibrating device for fluorescence immunoassay quantitative analysis appearance |
| CN117929345A (en) * | 2024-03-22 | 2024-04-26 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
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| CN117929345A (en) * | 2024-03-22 | 2024-04-26 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
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Application publication date: 20150902 |