A kind of light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance
Technical field
The utility model belongs to the other POCT (Point-Of-care Testing) of detection of bed check field, is specifically related to a kind of light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance.
Background technology
[0002] there is contradiction in present POCT fluorescent inspection field always between fluoroscopic examination efficient and instrument volume.Reach higher fluoroscopic examination efficient, instrument is just very big unavoidably.Be not suitable for the market orientation of POCT product.Based on there not being at present good product to change this difficult problem, the utility model utilizes a kind of light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance to solve the problems referred to above.
Summary of the invention
The utility model provides a kind of light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance, has solved the difficult problem that existing POCT fluorescent quantitation testing product fluorescence efficiency is not high or volume is excessive.
The technical scheme of the utility model is following:
A kind of light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance; Said light channel structure comprises laser instrument, two-phase dichronic mirror, first plano-convex lens, second plano-convex lens, optical filtering, anti-dazzling screen and photoelectric cell; Said two-phase dichronic mirror, first plano-convex lens, second plano-convex lens, optical filtering, anti-dazzling screen, photoelectric cell on an optical axis, said photoelectric cell, anti-dazzling screen, second plano-convex lens, optical filtering, two-phase dichronic mirror and first plano-convex lens successively according to from top to bottom according to order put.
Said laser instrument constitutes excitation light path, and two-phase dichronic mirror, first plano-convex lens, second plano-convex lens, optical filtering, anti-dazzling screen and photoelectric cell constitute fluorescence detection optical path, and excitation light path and fluorescence detection optical path are on same optical axis.
Laser arrives fluorescence excitation material on the thing to be excited through two-phase dichronic mirror, first plano-convex lens, anti-dazzling screen, and fluorescent material is through first plano-convex lens, via optical filtering, second plano-convex lens arrival photoelectric cell.
Said two-phase dichronic mirror and laser instrument are on same horizontal line, and the placement angle of two-phase dichronic mirror is 45 °.
Distance between said first plano-convex lens and the determinand equals the focal length of first plano-convex lens, and second plano-convex lens and photronic distance are near the focal length of second plano-convex lens.
The beneficial effect of the utility model is:
The utility model provides a kind of light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance, has solved the difficult problem that existing POCT fluorescent quantitation testing product fluorescence efficiency is not high or volume is excessive.
The excitation light path of the light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance that the utility model provides and fluorescence detection optical path are on same optical axis.The circuit and the corollary apparatus that match with said light channel structure use simultaneously, when detecting fluorescence signal, have very high fluoroscopic examination efficient and stability.
Description of drawings:
Fig. 1 is the light path facility split structural representation of the utility model.
Fig. 2 is the light channel structure synoptic diagram of the utility model.
Fig. 3 is the utility model light path facility assembly structure synoptic diagram.
Embodiment:
Below in conjunction with accompanying drawing and embodiment the utility model is done further detailed explanation.
Like Fig. 1, Fig. 2, shown in Figure 3; A kind of light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance; Said light channel structure comprises laser instrument 2, two-phase dichronic mirror 3, first plano-convex lens 4, second plano-convex lens 5, optical filtering 6, anti-dazzling screen 7 and photoelectric cell 8; Said two-phase dichronic mirror 3, first plano-convex lens 4, second plano-convex lens 5, optical filtering 6, anti-dazzling screen 7, photoelectric cell 8 on an optical axis, photoelectric cell 8, anti-dazzling screen 7, second plano-convex lens 5, optical filtering 6, two-phase dichronic mirror 3 and first plano-convex lens 4 successively according to from top to bottom according to order put.
Laser instrument 2 constitutes excitation light paths, and two-phase dichronic mirror 3, first plano-convex lens 4, second plano-convex lens 5, optical filtering 6, anti-dazzling screen 7 and photoelectric cell 8 constitute fluorescence detection optical paths, and excitation light path and fluorescence detection optical path are on same optical axis.
Laser arrives fluorescence excitation material on the thing to be excited through two-phase dichronic mirror 3, first plano-convex lens 4, anti-dazzling screen 7, and fluorescent material is through first plano-convex lens 4, arrive photoelectric cells via optical filtering 6, second plano-convex lens 5.
Two-phase dichronic mirror 3 and laser instrument 2 are on same horizontal line, and the placement angle of two-phase dichronic mirror 3 is 45 °.
Distance between first plano-convex lens 4 and the determinand equals the focal length of first plano-convex lens 4, and the convex surface of first plano-convex lens 4 is downward.
The distance of second plano-convex lens 5 and photoelectric cell 8 is near the focal length of second plano-convex lens 5, and the convex surface of second plano-convex lens 5 upwards.
A kind of preparation method who is applied to the light channel structure of real-time immunofluorescence detection by quantitative appearance comprises the steps:
1) first design light channel structure facility, according to the size of appointment with require die sinking to be prepared from;
2) laser instrument 2, two-phase dichronic mirror 3, first plano-convex lens 4, second plano-convex lens 5, optical filtering 6, anti-dazzling screen 7, photoelectric cell 8 are put into the groove of light channel structure facility according to correct putting.
3) the light channel structure facility is closed tightly, then lock screw.
The light channel structure that is applied to real-time immunofluorescence detection by quantitative appearance of the utility model has very high fluoroscopic examination efficient and stability when detecting fluorescence signal.Solved the difficult problem that existing POCT fluorescent quantitation testing product fluorescence efficiency is not high or volume is excessive.
Said light channel structure facility can come out through mold injection.
Said light channel structure facility material can be selected engineering plastics such as ABS, PP.
The above; It only is the preferred embodiment of the utility model; Be not to be the restriction of the utility model being made other form, any professional and technical personnel of being familiar with possibly utilize the technology contents of above-mentioned announcement to change or be modified as the equivalent embodiment of equivalent variations.But every the utility model technical scheme content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment did, still belongs to the protection domain of the utility model technical scheme according to the technical spirit of the utility model.