CN203981589U - A kind of integrated seawater chlorophyll micro flow chip sensor - Google Patents

A kind of integrated seawater chlorophyll micro flow chip sensor Download PDF

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Publication number
CN203981589U
CN203981589U CN201420413185.4U CN201420413185U CN203981589U CN 203981589 U CN203981589 U CN 203981589U CN 201420413185 U CN201420413185 U CN 201420413185U CN 203981589 U CN203981589 U CN 203981589U
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micro flow
chlorophyll
layer
led chip
reflection
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赵强
刘世萱
王波
张可可
徐宇柘
闫星魁
王文彦
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Oceanographic Instrumentation Research Institute Shandong Academy of Sciences
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Oceanographic Instrumentation Research Institute Shandong Academy of Sciences
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Abstract

A kind of integrated seawater chlorophyll micro flow chip sensor that strengthens antijamming capability, improves the detection efficiency of fluorescence.Technical scheme is: it is characterized in that by Si-PIN detector (1), 6H-SiC/ITO/SiO 2layer (3), reflection and weld layer (4), LED chip (5), glass plate (6), PDMS microchannel (7) and metallic-membrane plating reflector (8) composition, wherein, described Si-PIN detector (1), 6H-SiC/ITO/SiO 2layer (3), reflection and weld layer (4), LED chip (5), glass plate (6), PDMS microchannel (7) and metallic-membrane plating reflector (8) are by being linked in sequence from top to bottom.

Description

A kind of integrated seawater chlorophyll micro flow chip sensor
Technical field
The utility model belongs to seawater chlorophyll micro flow chip field of sensor devices, especially a kind of integrated seawater chlorophyll micro flow chip sensor that strengthens antijamming capability, improves the detection efficiency of fluorescence.
Background technology
Chlorophyll content is an important indicator of reflection seawater ecologic environment, can estimate accordingly the situation such as ecology and pollution of marine environment, and therefore the chlorophyllous fast and accurate measurement of seawater is significant.
At present, comparatively typical classic method is shown in patent 201110271796.0, system is made up of LED array light source, lens combination, visible filter, condenser lens (I and II), bandpass filter, sample cell and PMT, integrated component relatively, this system bulk is large, complex structure, is subject to the interference of external shock etc., and the modulation of system rear end complexity and data processing section have also improved system cost; In addition, the light path of transmission-type cannot be avoided exciting light interference to fluorescence in 685nm punishment amount.
For above problem, we have proposed a kind of seawater chlorophyll detection method based on micro flow chip, the method is utilized novel micro flow chip technology, the light source of system, reaction tank and detecting area are integrated on a chip, simplify system architecture, dwindle system bulk, strengthened system rejection to disturbance ability; Detector is positioned at light source structure below and has avoided the interference of exciting light to fluorescence, has improved the detection efficiency of fluorescence.
Utility model content
The purpose of this utility model is to provide a kind of integrated seawater chlorophyll micro flow chip sensor that strengthens antijamming capability, improves the detection efficiency of fluorescence.
The technical solution of the utility model is: a kind of integrated seawater chlorophyll micro flow chip sensor, is characterized in that by Si-PIN detector (1), 6H-SiC/ITO/SiO 2layer (3), reflection and weld layer (4), LED chip (5), glass plate (6), PDMS microchannel (7) and metallic-membrane plating reflector (8) composition, wherein, described Si-PIN detector (1), 6H-SiC/ITO/SiO 2layer (3), reflection and weld layer (4), LED chip (5), glass plate (6), PDMS microchannel (7) and metallic-membrane plating reflector (8) are by being linked in sequence from top to bottom.
On described reflection and weld layer (4), be provided with the LED chip (5) of multiple identical wavelength, improve the intensity of excitation source.
On described reflection and weld layer (4), be provided with the LED chip (5) of multiple different wave lengths, according to the chlorophyllous fluorescence excitation characteristic of variety classes, realize the synchronizing detection to multiple chlorophyll composition.
Described glass plate (6) and PDMS microchannel (7) have formed the reaction tank of laser-induced fluorescence (LIF), the capacity gauge for enhancing system to fluorescence signal.
Effect of the present utility model is: integrated seawater chlorophyll micro flow chip sensor, and by Si-PIN detector, 6H-SiC/ITO/SiO 2layer, reflection and weld layer, LED chip, glass plate, PDMS microchannel and metallic-membrane plating reflector composition, wherein, described Si-PIN detector, 6H-SiC/ITO/SiO 2layer, reflection and weld layer, LED chip, glass plate, PDMS microchannel and metallic-membrane plating reflector are by being linked in sequence from top to bottom.
The utlity model has following features: (1) system detection efficiency is high.Because LED chip volume is little, be convenient to array integrated, therefore can improve excitation source intensity by the mode that multiple LED chips are integrated.Utilize microchannel structure can strengthen the capacity gauge of system to fluorescence, adopt the mode of plated film that the fluorescence of transmission is reflected back to detector again simultaneously, avoid the transmission loss of fluorescence, strengthened the intensity of fluorescence signal, finally improved the detection efficiency of system.
(2) can survey multiple chlorophyll simultaneously.According to also not identical characteristic of its excitation source characteristic wavelength of different chlorophyll and wavelength of fluorescence, the LED chip of multiple wavelength can be integrated together, as structure in Fig. 32 and 3 is the LED chip of different excitation wavelengths.As long as change corresponding filter plate and detector, can realize the multiple chlorophyll of synchronizing detection for different wavelength of fluorescence;
(3) system sealing performance is good, reliability is high, and anti-ocean severe environment ability is strong.Light source adopts LED chip to replace traditional LED bulb, light source, detector and microchannel height are integrated simultaneously, avoid the lens combination in conventional light path, when having simplified light path system, also reduced the volume of system, strengthened the antidetonation of system, waterproof ability, has improved the adaptive faculty of system to ocean severe environment.
Brief description of the drawings
Fig. 1 is the utility model one example structure schematic diagram;
Fig. 2 is another example structure schematic diagram of the utility model.
Embodiment
In Fig. 1, a kind of integrated seawater chlorophyll micro flow chip sensor, by Si-PIN detector 1,6H-SiC/ITO/SiO 2layer 3, reflection and weld layer 4, LED chip 5, glass plate 6, PDMS microchannel 7 and metallic-membrane plating reflector 8 form, wherein, and described Si-PIN detector 1,6H-SiC/ITO/SiO 2layer 3, reflection and weld layer 4, LED chip 5, glass plate 6, PDMS microchannel 7 and metallic-membrane plating reflector 8 are by being linked in sequence from top to bottom.Wherein, the 2nd, detector active region, the 9th, chlorophyll molecule.
Reflectance coating 8 is positioned at the top of PDMS microchannel 7, and reflectance coating 8 can reflecting and collecting fluorescence, allows exciting light to see through simultaneously.Si-PIN detector 1 is positioned at LED chip below, for the detection of fluorescence, 6H-SiC/ITO/SiO 22 tops, active area that layer 3 is positioned at Si-PIN detector 1, for seeing through and the filtration of exciting light of fluorescence.
On reflection and weld layer 4, can be provided with the LED chip 5 of multiple identical wavelength, improve the intensity of excitation source.
On reflection and weld layer 4, can be provided with the LED chip 5 of multiple different wave lengths, according to the chlorophyllous fluorescence excitation characteristic of variety classes, realize the synchronizing detection (referring to Fig. 2) to multiple chlorophyll composition.
Glass plate 6 and PDMS microchannel 7 have formed the reaction tank of laser-induced fluorescence (LIF), the capacity gauge for enhancing system to fluorescence signal.
Above-described specific embodiment; the purpose of this utility model, technical scheme and beneficial effect are further described; institute is understood that; the foregoing is only specific embodiment of the utility model; be not limited to the utility model; all within spirit of the present utility model and principle, any amendment of making, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.

Claims (4)

1. an integrated seawater chlorophyll micro flow chip sensor, is characterized in that by Si-PIN detector (1), 6H-SiC/ITO/SiO 2layer (3), reflection and weld layer (4), LED chip (5), glass plate (6), PDMS microchannel (7) and metallic-membrane plating reflector (8) composition, wherein, described Si-PIN detector (1), 6H-SiC/ITO/SiO 2layer (3), reflection and weld layer (4), LED chip (5), glass plate (6), PDMS microchannel (7) and metallic-membrane plating reflector (8) are by being linked in sequence from top to bottom.
2. integrated seawater chlorophyll micro flow chip sensor according to claim 1, its feature is being the LED chip (5) that is provided with multiple identical wavelength on described reflection and weld layer (4), improves the intensity of excitation source.
3. the integrated seawater chlorophyll of one according to claim 1 micro flow chip sensor, it is characterized in that being provided with on described reflection and weld layer (4) LED chip (5) of multiple different wave lengths, according to the chlorophyllous fluorescence excitation characteristic of variety classes, realize the synchronizing detection to multiple chlorophyll composition.
4. the integrated seawater chlorophyll of one according to claim 1 micro flow chip sensor, is characterized in that described glass plate (6) and PDMS microchannel (7) have formed the reaction tank of laser-induced fluorescence (LIF), the capacity gauge for enhancing system to fluorescence signal.
CN201420413185.4U 2014-07-25 2014-07-25 A kind of integrated seawater chlorophyll micro flow chip sensor Active CN203981589U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104101588A (en) * 2014-07-25 2014-10-15 山东省科学院海洋仪器仪表研究所 Integrated seawater chlorophyll microfluidic chip sensor and manufacturing method thereof
US9735305B2 (en) 2015-09-21 2017-08-15 International Business Machines Corporation Monolithically integrated fluorescence on-chip sensor
CN112946036A (en) * 2021-02-01 2021-06-11 浙江大学 Electrochemical modified electrode and preparation method and application thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104101588A (en) * 2014-07-25 2014-10-15 山东省科学院海洋仪器仪表研究所 Integrated seawater chlorophyll microfluidic chip sensor and manufacturing method thereof
US9735305B2 (en) 2015-09-21 2017-08-15 International Business Machines Corporation Monolithically integrated fluorescence on-chip sensor
CN112946036A (en) * 2021-02-01 2021-06-11 浙江大学 Electrochemical modified electrode and preparation method and application thereof
CN112946036B (en) * 2021-02-01 2022-10-21 浙江大学 Electrochemical modified electrode and preparation method and application thereof

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