CN201935855U - Dual-energy laser system - Google Patents

Dual-energy laser system Download PDF

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Publication number
CN201935855U
CN201935855U CN2011200039766U CN201120003976U CN201935855U CN 201935855 U CN201935855 U CN 201935855U CN 2011200039766 U CN2011200039766 U CN 2011200039766U CN 201120003976 U CN201120003976 U CN 201120003976U CN 201935855 U CN201935855 U CN 201935855U
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China
Prior art keywords
light emitter
lasing light
laser
converging device
receiving trap
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Expired - Lifetime
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CN2011200039766U
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Chinese (zh)
Inventor
党福喜
王彩荣
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SHENZHEN MAXCOM ELECTRONIC CO Ltd
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SHENZHEN MAXCOM ELECTRONIC CO Ltd
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Abstract

The utility model provides a dual-energy laser system, which belongs to the field of an optical system of a cell analysis device. The dual-energy laser system comprises a first laser source, a second laser source, a first converging device, a second converging device, a sample chamber, a front receiving device and a lateral receiving device; the laser emitted by the first laser source radiates into the sample chamber after being converged by the first converging device; the laser emitted by the second laser source radiates into the sample chamber after being converged by the second converging device; moreover, generated scattered lights are respectively received by the front receiving device and the lateral receiving device. The dual-energy laser system is mainly applied to the optical system of a blood cell analyzer.

Description

The dual intensity laser system
Technical field
The utility model relates to the optical system of cell analysis equipment, relates in particular to the dual intensity laser system.
Background technology
In the blood cell analysis method, often need being used in combination of multiple detection technique that leucocyte is carried out five and hive off.As volume, electricity lead, laser scattering technology (VCS), impedance, laser light scattering and fluorescent dye technology, laser light scattering and cytochemical staining technology etc.The effect that laser scattering technology play more and more in blood cell analysis method is important.
Existing cytoanalyze often has only a LASER Light Source.The monoenergetic laser system of single light source can only obtain a cover and detect data from a direction irradiation sample, and the cell interior structural information of acquisition is limited.Because cell membrane also can produce light scattering, so cell membrane can obtaining of pair cell internal structural information produce interference.Especially hemolysin erythrocytic while in handling haemocyte, shrinkage can take place in leucocyte, makes the influence of leucocyte film more obvious.
The utility model content
For solving problems of the prior art, the utility model provides a kind of dual intensity laser system, can reduce the interference that cell membrane produces in the cell sample testing process, obtains more many cells internal structural information, carries out leucocyte five more accurately and hives off.
The utility model comprises first lasing light emitter, second lasing light emitter, first converging device, second converging device, sample chamber, forward direction receiving trap and side direction receiving trap; The described first lasing light emitter emitted laser is injected the sample chamber after first converging device converges, the described second lasing light emitter emitted laser is injected the sample chamber after second converging device converges, the scattered light of generation is received by forward direction receiving trap and side direction receiving trap respectively.
As further improvement of the utility model, described first converging device comprises first focus lamp; Described second converging device comprises second focus lamp.
As further improvement of the utility model, described first lasing light emitter is a semiconductor laser; Described first converging device further comprises first collimating mirror that is used to collimate first lasing light emitter emission laser.
As further improvement of the utility model, described second lasing light emitter is a semiconductor laser, and its emission optical maser wavelength is different with first lasing light emitter emission optical maser wavelength; Described second converging device further comprises second collimating mirror that is used to collimate second lasing light emitter emission laser.
As further improvement of the utility model, described forward direction receiving trap comprises forward direction focus lamp and forward direction photelectric receiver; Described side direction receiving trap comprises side direction focus lamp and side direction photelectric receiver.
As further improvement of the utility model, described forward direction receiving trap comprises that further the first two is to color chips; Described side direction receiving trap further comprises side dichroic sheet.
As further improvement of the utility model, described dual intensity laser system further comprises anti-dazzling screen, lays respectively at intersection point and second lasing light emitter emitted laser the intersection point place through second converging device converge after of the first lasing light emitter emitted laser after first converging device converges.
The utility model comprises two lasing light emitters, and first lasing light emitter emission Wavelength of Laser is different with second lasing light emitter emission Wavelength of Laser, so be the dual intensity laser system.
Compared with prior art, the beneficial effects of the utility model are: the dual intensity laser system is used for blood cell analysis, simple in structure, can obtain the detection data of first lasing light emitter and second lasing light emitter respectively, by being detected data, two covers carry out separate analysis and correspondence analysis, can reduce the interference that cell membrane produces, obtain more many cells internal structural information, carry out leucocyte five more accurately and hive off.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment one.
Fig. 2 is the structural representation of the utility model embodiment two.
Embodiment
Below in conjunction with drawings and Examples the utility model is described in further details.
Embodiment one: as shown in Figure 1, the utility model comprises first lasing light emitter 1, second lasing light emitter 2, first converging device 3, second converging device 4, sample chamber 5, anti-dazzling screen 6, forward direction receiving trap 7 and side direction receiving trap 8; First lasing light emitter, 1 emitted laser is injected sample chamber 5 after first converging device 3 converges, second lasing light emitter, 2 emitted laser are injected sample chamber 5 after second converging device 4 converges, the scattered light of generation is received by forward direction receiving trap 7 and side direction receiving trap 8 respectively.
First lasing light emitter 1 and second lasing light emitter 2 are semiconductor laser, and first lasing light emitter, 1 emission Wavelength of Laser is greater than second lasing light emitter, 2 emission Wavelength of Laser.Effects such as laser light scattering can take place in laser radiation to cell.In the light scattering process, light wavelength does not change, and the scattering light wavelength is identical with the wavelength of incident laser.
First converging device 3 comprises first collimating mirror 31, first focus lamp 32, and presses the direction of propagation placement of incident light successively.Second converging device 4 comprises second collimating mirror 41, second focus lamp 42 and semi-transparent semi-reflecting lens 43, and presses the direction of propagation placement of incident light successively.Semi-transparent semi-reflecting lens 43 is positioned at the intersection of first lasing light emitter, 1 emission optical propagation direction and second lasing light emitter, 2 emission optical propagation directions.Intersection point behind first lasing light emitter, the 1 emission light transmission semi-transparent semi-reflecting lens 43 and the intersection point of second lasing light emitter, 2 emission light after semi-transparent semi-reflecting lens 43 reflections are in same point.Forward direction receiving trap 7 comprises forward direction focus lamp 71, the first two printing opacity electricity receiver 73, preceding reflective electric receiver 74 before color chips 72 reaches.Side direction receiving trap 8 comprises side direction focus lamp 81, side dichroic sheet 82 and side printing opacity electricity receiver 83, the reflective electric receiver 84 of side.In the scattered light, the long light of wavelength transmission is respectively crossed the first two to color chips 72, side dichroic sheet 82, and the short light of wavelength respectively by the first two to color chips 72,82 reflections of side dichroic sheet.
First lasing light emitter, 1 emitted laser is through first collimating mirror, 31 collimations, and after first focus lamp 32 converged, through semi-transparent semi-reflecting lens 43 transmissions, forward was injected the stream of cells in the sample chamber 5, and effects such as laser light scattering take place.Forward scattering light wherein converges through forward direction focus lamp 71, sees through the first two to color chips 72, printing opacity electricity receiver 73 before arriving.Forward scattering only the phalangeal cell scattering send slightly be the scattered light of parallel direction with incident laser.Can obtain the characteristic information of cell size by the detection of forward scattering light.Side scattered light converges through side direction focus lamp 81, sees through side dichroic sheet 82, arrives side printing opacity electricity receiver 83.Side scattered light be the phalangeal cell scattering send with incident laser scattered light slightly in vertical direction.Can obtain the characteristic information of cell interior structure by the detection of side scattered light.
Second lasing light emitter, 2 emitted laser are through second collimating mirror 41 collimation, after second focus lamp 42 converges, through semi-transparent semi-reflecting lens 43 reflections, inject stream of cells the sample chamber 5 with first lasing light emitter, 1 emitted laser from same direction, and effects such as laser light scattering take place.Forward scattering light wherein converges through forward direction focus lamp 71, reflects reflective electric receiver 74 before arriving again to color chips 72 through the first two.Side scattered light converges through side direction focus lamp 81, through 82 reflections of side dichroic sheet, arrives the reflective electric receiver 84 of side.Anti-dazzling screen 6 is positioned at first lasing light emitter, 1 emission light and second lasing light emitter, 2 radiative intersection point places, is used to stop the surplus light after incident laser sees through sample chamber 5.
When the sample in the sample chamber 5 can't make fluorescence excitation without fluorescence labeling or first lasing light emitter 1 and second lasing light emitter, 2 emitted laser, preceding printing opacity electricity receiver 73, preceding reflective electric receiver 74 and side printing opacity electricity receiver 83, the reflective electric receiver 84 of side received only scattered light signal.The first two is used for the separation of different wave length scattered light to color chips 72, side dichroic sheet 82.
When the sample in the sample chamber 5 through fluorescence labeling and can be excited by first lasing light emitter 1 and second lasing light emitter, 2 emitted laser the time, the forward direction fluorescence of emission converges through forward direction focus lamp 71, see through the first two to color chips 72, after optical filter is removed scattered light, received by preceding printing opacity electricity receiver 73; The side direction fluorescence of emission converges through side direction focus lamp 81, sees through side dichroic sheet 82, after optical filter is removed scattered light, is received by side printing opacity electricity receiver 83.Forward scattering light converges through forward direction focus lamp 71,, is received or further receives after the dichroic sheet separates again by preceding reflective electric receiver 74 to color chips 72 reflections through the first two.Side scattered light converges through side direction focus lamp 81, through 82 reflections of side dichroic sheet, is received or further receives after the dichroic sheet separates again by the reflective electric receiver 84 of side.The first two is used for separating of fluorescence and scattered light to color chips 72, side dichroic sheet 82, and the wavelength of fluorescence is greater than the scattering light wavelength.
Embodiment two: as shown in Figure 2, the utility model comprises first lasing light emitter 1, second lasing light emitter 2, first converging device 3, second converging device 4, sample chamber 5, anti-dazzling screen 6,61, forward direction receiving trap 7 and side direction receiving trap 8; First lasing light emitter, 1 emitted laser converges through first converging device 3, and second lasing light emitter, 2 emitted laser are injected sample chamber 5 after second converging device 4 converges, and the scattered light of generation is received by forward direction receiving trap 7 and side direction receiving trap 8 respectively.
First lasing light emitter 1 and second lasing light emitter 2 are semiconductor laser, and first lasing light emitter, 1 emission optical maser wavelength is greater than second lasing light emitter, 2 emission optical maser wavelengths.Effects such as laser light scattering can take place in laser radiation to cell.In the light scattering process, light wavelength does not change, and the scattering light wavelength is identical with the wavelength of incident laser.
First converging device 3 comprises first collimating mirror 31, first focus lamp 32, and presses the direction of propagation placement of incident light successively.Second converging device 4 comprises second collimating mirror 41, second focus lamp 42, and presses the direction of propagation placement of incident light successively.Forward direction receiving trap 7 comprises forward direction focus lamp 71, the first two printing opacity electricity receiver 73, preceding reflective electric receiver 74 before color chips 72 reaches.Side direction receiving trap 8 comprises side direction focus lamp 81, side dichroic sheet 82 and side printing opacity electricity receiver 83, the reflective electric receiver 84 of side.In the scattered light, the long light of wavelength transmission is respectively crossed the first two to color chips 72, side dichroic sheet 82, and the short light of wavelength respectively by the first two to color chips 72,82 reflections of side dichroic sheet.
First lasing light emitter, 1 emitted laser is through first collimating mirror, 31 collimations, and after first focus lamp 32 converged, forward was injected the stream of cells in the sample chamber 5, and effects such as laser light scattering take place.Forward scattering light converges through forward direction focus lamp 71, sees through the first two to color chips 72, printing opacity electricity receiver 73 before arriving.Side scattered light converges through side direction focus lamp 81, sees through side dichroic sheet 82, arrives side printing opacity electricity receiver 83.Anti-dazzling screen 6 is positioned at the intersection point place of first lasing light emitter, 1 emission laser, is used to stop the surplus light after incident laser sees through sample chamber 5.
Second lasing light emitter, 2 emitted laser are through second collimating mirror 41 collimation, after second focus lamp 42 converges, inject stream of cells in the sample chamber 5 in vertical direction with first lasing light emitter, 1 emitted laser, and effects such as laser light scattering take place.Forward scattering light converges through forward direction focus lamp 81, to color chips 82 reflections, arrives preceding reflective electric receiver 84 through the first two.Side scattered light converges through side direction focus lamp 71, through 72 reflections of side dichroic sheet, arrives the reflective electric receiver 74 of side.Anti-dazzling screen 61 is positioned at the intersection point place of second lasing light emitter, 2 emission laser, is used to stop the surplus light after incident laser sees through sample chamber 5.
When the sample in the sample chamber 5 can't make fluorescence excitation without fluorescence labeling or first lasing light emitter 1 and second lasing light emitter, 2 emitted laser, preceding printing opacity electricity receiver 73, preceding reflective electric receiver 74 and side printing opacity electricity receiver 83, the reflective electric receiver 84 of side received only scattered light signal.The first two is used for the separation of different wave length scattered light to color chips 72, side dichroic sheet 82.
When the sample in the sample chamber 5 through fluorescence labeling and can be excited by first lasing light emitter 1 and second lasing light emitter, 2 emitted laser the time, the forward direction fluorescence of emission converges through forward direction focus lamp 71, see through the first two to color chips 72, after optical filter is removed scattered light, received by preceding printing opacity electricity receiver 73; The side direction fluorescence of emission converges through side direction focus lamp 81, sees through side dichroic sheet 82, after optical filter is removed scattered light, is received by side printing opacity electricity receiver 83.Forward scattering light converges through forward direction focus lamp 71,, is received or further receives after the dichroic sheet separates again by preceding reflective electric receiver 74 to color chips 72 reflections through the first two.Side scattered light converges through side direction focus lamp 81, through 82 reflections of side dichroic sheet, is received or further receives after the dichroic sheet separates again by the reflective electric receiver 84 of side.The first two is used for separating of fluorescence and scattered light to color chips 72, side dichroic sheet 82, and the wavelength of fluorescence is greater than the scattering light wavelength.
Above content be in conjunction with concrete preferred implementation to further describing that the utility model is done, can not assert that concrete enforcement of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field, under the prerequisite that does not break away from the utility model design, can also make several simple deduction or replace, all should be considered as belonging to protection domain of the present utility model.

Claims (7)

1. a dual intensity laser system is characterized in that: comprise first lasing light emitter, second lasing light emitter, first converging device, second converging device, sample chamber, forward direction receiving trap and side direction receiving trap; The described first lasing light emitter emitted laser is injected the sample chamber after first converging device converges, the described second lasing light emitter emitted laser is injected the sample chamber after second converging device converges, the scattered light of generation is received by forward direction receiving trap and side direction receiving trap respectively.
2. dual intensity laser system according to claim 1 is characterized in that: described first converging device comprises first focus lamp; Described second converging device comprises second focus lamp.
3. dual intensity laser system according to claim 2 is characterized in that: described first lasing light emitter is a semiconductor laser; Described first converging device further comprises first collimating mirror that is used to collimate first lasing light emitter emission laser.
4. dual intensity laser system according to claim 2 is characterized in that: described second lasing light emitter is a semiconductor laser, and its emission optical maser wavelength is different with first lasing light emitter emission optical maser wavelength; Described second converging device further comprises second collimating mirror that is used to collimate second lasing light emitter emission laser.
5. dual intensity laser system according to claim 1 is characterized in that: described forward direction receiving trap comprises forward direction focus lamp and forward direction photelectric receiver; Described side direction receiving trap comprises side direction focus lamp and side direction photelectric receiver.
6. dual intensity laser system according to claim 5 is characterized in that: described forward direction receiving trap comprises that further the first two is to color chips; Described side direction receiving trap further comprises side dichroic sheet.
7. dual intensity laser system according to claim 1, it is characterized in that: described dual intensity laser system further comprises anti-dazzling screen, lays respectively at intersection point and second lasing light emitter emitted laser the intersection point place through second converging device converge after of the first lasing light emitter emitted laser after first converging device converges.
CN2011200039766U 2011-01-07 2011-01-07 Dual-energy laser system Expired - Lifetime CN201935855U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103063626A (en) * 2012-12-13 2013-04-24 江西科技师范大学 Light path auto-correction cell laser excitation detecting device and detecting method thereof
CN104067105A (en) * 2011-09-14 2014-09-24 马尔文仪器有限公司 Apparatus and method for measuring particle size distribution by light scattering
CN109238965A (en) * 2018-09-25 2019-01-18 军事科学院系统工程研究院卫勤保障技术研究所 A kind of dry chemical blood cell composition analysis optical system

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104067105A (en) * 2011-09-14 2014-09-24 马尔文仪器有限公司 Apparatus and method for measuring particle size distribution by light scattering
US9869625B2 (en) 2011-09-14 2018-01-16 Malvern Instruments Limited Apparatus and method for measuring particle size distribution by light scattering
CN103063626A (en) * 2012-12-13 2013-04-24 江西科技师范大学 Light path auto-correction cell laser excitation detecting device and detecting method thereof
CN109238965A (en) * 2018-09-25 2019-01-18 军事科学院系统工程研究院卫勤保障技术研究所 A kind of dry chemical blood cell composition analysis optical system

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Granted publication date: 20110817