CN103868902A - Bioluminescence detection probe - Google Patents
Bioluminescence detection probe Download PDFInfo
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- CN103868902A CN103868902A CN201410132743.4A CN201410132743A CN103868902A CN 103868902 A CN103868902 A CN 103868902A CN 201410132743 A CN201410132743 A CN 201410132743A CN 103868902 A CN103868902 A CN 103868902A
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Abstract
The invention relates to a bioluminescence detection probe which comprises a photoelectric sensor, a mounting rack for holding lenses, and a moving scanning platform for holding a biologic reagent to be tested. The bioluminescence detection probe is characterized in that light sources are symmetrically arranged on two sides of the mounting rack relative to a central line; emergent rays from the light sources on the two sides are intersected onto the biologic reagent to be tested; the first lenses are respectively and symmetrically arranged on light paths between each of the light sources on two sides and the biologic reagent to be tested; rays triggered by radiation of the rays of the light sources on the two sides on the biologic reagent to be tested further pass the second lens and a light filter arranged on the mounting rack to reach a photoelectric sensor. The bioluminescence detection probe is good in detection effect, can take the place of a probe part of a conventional bioluminescence detection system to a certain extent, and has a good prompting function for popularization and application of a bioluminescence detection technology.
Description
Technical field
The present invention relates to a kind of bioluminescence detection probe, belong to the technical fields such as Photoelectric Detection, bioluminescence detection and analysis.
Background technology
It is a kind of powerful analysis means that fluorescence analysis detects, and is widely used in clinical examination, biological study, agricultural sciences, food and environmental science.Under room temperature, the lowest vibration energy level of most of molecules in ground state, after molecule absorption energy (luminous energy, chemical energy, electric energy or heat energy) in ground state, transit to excited state, excited state is unstable, to decay to very soon ground state, form with light is emitted energy, and this phenomenon is referred to as " luminescence phenomenon ".Fluorescence analysis detection technique is exactly a kind of form of luminescence phenomenon, utilizes high energy particle irradiating biological reagent, thereby the fluorescent material that biological reagent contains can be excited and sends fluorescence, by detecting fluorescence, the composition of biological reagent is analyzed.Fluorescence intensity is directly proportional to the concentration of fluorescent material, by the fluorescence detecting, just can carry out to some composition the analysis of quantitative and qualitative analysis.
But the probe light path complexity that existing bioluminescence detection technique is used causes equipment very too fat to move, causes bioluminescence pick-up unit volume very much not to utilize and carries and operate use.
Summary of the invention
In view of the deficiencies in the prior art, the present invention, by appropriate design, optimization light path, proposes a kind of bioluminescence detection probe of small size easy operating, makes bioluminescence checkout equipment volume, weight significantly reduce to be easier to industry and promotes.
Technical program of the present invention lies in:
A kind of bioluminescence detection probe, comprise photoelectric sensor, in order to install the erecting frame of lens and in order to lay the motion scan platform of biological reagent to be measured, it is characterized in that: the relative center line in both sides of described erecting frame is arranged with a light source, the emergent ray of two side light sources intersects on biological reagent to be measured, in light path between described two side light sources and biological reagent to be measured, be arranged with respectively a first lens, on biological reagent to be measured, irradiate the light inspiring again successively through installing one second lens on erecting frame through both sides source light, one optical filter arrives photoelectric sensor.
Wherein, described light source center position, both sides becomes isosceles right triangle relation with the position of biological reagent to be measured, the center of described first lens lays respectively on the center of optical path distance between respective side light source center and biological reagent to be measured, described biological reagent to be measured, photoelectric sensor and be positioned at locational second lens of equity in the middle of both and coexist on a straight line, described photoelectric sensor is positioned at the central part of two side light source connecting lines.
The center of described light source is the twice of first lens focal length to the distance at respective side first lens center.
Be positioned at light source and offer the diaphragm duct that aperture is 2-2.5mm to the erecting frame in respective side first lens light path.
The invention has the advantages that: the present invention detects respond well, can replace to a certain extent the probe segment of existing bioluminescence detection system, for the good impetus of applying of bioluminescence detection technique.
Accompanying drawing explanation
Fig. 1 is the structural representation of the embodiment of the present invention.
Fig. 2 is the multiple isosceles right triangle structural representation of the embodiment of the present invention.
Fig. 3 is the plan structure schematic diagram of the erecting frame of the embodiment of the present invention.
Fig. 4 is the member of the embodiment of the present invention positional structure schematic diagram on erecting frame.
Embodiment
For above-mentioned feature and advantage of the present invention can be become apparent, special embodiment below, and coordinate accompanying drawing, be described in detail below.
Referring to figs. 1 to Fig. 4, the present invention relates to a kind of bioluminescence detection probe, comprise photoelectric sensor 8, in order to install the erecting frame 7 of lens and in order to lay the motion scan platform 10 of biological reagent 9 to be measured, the relative center line in both sides of described erecting frame 7 is arranged with a light source 1, 2, two side light sources 1, 2 emergent ray intersects on biological reagent 9 to be measured, two described side light sources 1, 2 and biological reagent to be measured 9 between light path on be arranged with respectively a first lens 3, 4, on biological reagent to be measured, irradiate the light inspiring again successively through installing one second lens 5 on erecting frame 7 through both sides source light, one optical filter 6 arrives photoelectric sensor 8.
Above-mentioned light source center position, both sides becomes isosceles right triangle relation with the position of biological reagent to be measured, the center of described first lens lays respectively on the center of optical path distance between respective side light source center and biological reagent to be measured, described biological reagent to be measured, photoelectric sensor and be positioned at locational second lens of equity in the middle of both and coexist on a straight line, described photoelectric sensor is positioned at the central part of two side light source connecting lines.
The center of above-mentioned light source is the twice of first lens focal length to the distance at respective side first lens center.
Be positioned at light source and offer the diaphragm duct 11 that aperture is 2-2.5mm to the erecting frame in respective side first lens light path.
Specific implementation process: this probe comprises light source 1,2, first lens 3,4, the second lens 5, optical filter 6, erecting frame 7, sensor 8, biological reagent to be measured 9, motion scan platform 10.Adopt double-light-source structuredly, increase light source emissive power, improve detection system accuracy of detection.Light source 1, 2 are arranged on erecting frame 7 and mark 1, 2 position, first lens 3, 4 are arranged on erecting frame 7 and mark 3, 4 position, the second lens 5 are arranged on the position that marks 5 on erecting frame 7, optical filter 6 is arranged on the position that marks 6 on erecting frame 7, erecting frame 7 is optical element installation supports, its size is through optical principle design, design philosophy is as follows: element 1, 2, 3, 4, 5, 8, 9 form multiple isosceles right triangle (in figure, each triangle is isosceles right triangle) as shown in Figure 2, the twice of the focal length that the size of isosceles right triangle is first lens by 1 to 3 distance is determined, utilize the symmetric property of isosceles right triangle, 1, 2 two light sources of locating send purple light through being put in 3, 4 liang of lens focus of locating are at biological reagent to be measured 9 places, excite it to send fluorescence, the photoelectric sensor that the lens focus that fluorescence is upwards placed in 5 places is placed in 8 places through its upper optical filter optical filtering detects.The aperture of simultaneously designing both sides aperture is that 2-2.5mm plays iris action.Motion scan platform 10 is for mobile and location biological reagent 9.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (4)
1. a bioluminescence detection probe, comprise photoelectric sensor, in order to install the erecting frame of lens and in order to lay the motion scan platform of biological reagent to be measured, it is characterized in that: the relative center line in both sides of described erecting frame is arranged with a light source, the emergent ray of two side light sources intersects on biological reagent to be measured, in light path between described two side light sources and biological reagent to be measured, be arranged with respectively a first lens, on biological reagent to be measured, irradiate the light inspiring again successively through installing one second lens on erecting frame through both sides source light, one optical filter arrives photoelectric sensor.
2. a kind of bioluminescence detection probe according to claim 1, it is characterized in that: described light source center position, both sides becomes isosceles right triangle relation with the position of biological reagent to be measured, the center of described first lens lays respectively on the center of optical path distance between respective side light source center and biological reagent to be measured, described biological reagent to be measured, photoelectric sensor and be positioned at locational second lens of equity in the middle of both and coexist on a straight line, described photoelectric sensor is positioned at the central part of two side light source connecting lines.
3. a kind of bioluminescence detection probe according to claim 1, is characterized in that: the center of described light source is the twice of first lens focal length to the distance at respective side first lens center.
4. a kind of bioluminescence detection probe according to claim 1, is characterized in that: be positioned at light source and offer the diaphragm duct that aperture is 2-2.5mm to the erecting frame in respective side first lens light path.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410132743.4A CN103868902B (en) | 2014-04-03 | 2014-04-03 | A kind of bioluminescence detection probe |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410132743.4A CN103868902B (en) | 2014-04-03 | 2014-04-03 | A kind of bioluminescence detection probe |
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| CN103868902A true CN103868902A (en) | 2014-06-18 |
| CN103868902B CN103868902B (en) | 2016-03-30 |
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| CN201410132743.4A Active CN103868902B (en) | 2014-04-03 | 2014-04-03 | A kind of bioluminescence detection probe |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105758834A (en) * | 2016-04-26 | 2016-07-13 | 福州大学 | Biochip detection method implemented through laser induction and CCD acquisition |
| CN106092995A (en) * | 2016-07-25 | 2016-11-09 | 天津水运工程勘察设计院 | A kind of indoor detection probe of fluorescent solutions concentration |
| CN106092994A (en) * | 2016-07-27 | 2016-11-09 | 福州大学 | A kind of micro-array chip fluorescence detection method of great power LED |
| CN107561043A (en) * | 2017-07-06 | 2018-01-09 | 复旦大学 | Biology sensor based on transform coding fluorescent technique on polychrome |
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| CN101553161A (en) * | 2006-10-13 | 2009-10-07 | 卡尔斯特里姆保健公司 | Apparatus for caries detection |
| CN102224410A (en) * | 2008-09-24 | 2011-10-19 | 施特劳斯控股公司 | Imaging analyzer for testing analytes |
| CN203287314U (en) * | 2013-04-28 | 2013-11-13 | 天津奇谱光电技术有限公司 | Multi-probe fluorescent sensing device |
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2014
- 2014-04-03 CN CN201410132743.4A patent/CN103868902B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1328639A (en) * | 1998-09-17 | 2001-12-26 | 程序控制公司 | X-ray fluorescence element analyzer |
| CN2491832Y (en) * | 2001-02-27 | 2002-05-15 | 成都理工学院 | Portable tube excited X-ray photofluorometer |
| CN1820082A (en) * | 2003-05-08 | 2006-08-16 | 生物-拉德实验室公司 | Systems and methods for fluorescence detection with a movable detection module |
| CN2655229Y (en) * | 2003-09-03 | 2004-11-10 | 西北有色地质研究院 | Multi-channel atomic fluorescent spectrograph |
| WO2005121864A2 (en) * | 2004-06-07 | 2005-12-22 | Fluidigm Corporation | Optical lens system and method for microfluidic devices |
| CN101553161A (en) * | 2006-10-13 | 2009-10-07 | 卡尔斯特里姆保健公司 | Apparatus for caries detection |
| CN102224410A (en) * | 2008-09-24 | 2011-10-19 | 施特劳斯控股公司 | Imaging analyzer for testing analytes |
| CN203287314U (en) * | 2013-04-28 | 2013-11-13 | 天津奇谱光电技术有限公司 | Multi-probe fluorescent sensing device |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105758834A (en) * | 2016-04-26 | 2016-07-13 | 福州大学 | Biochip detection method implemented through laser induction and CCD acquisition |
| CN105758834B (en) * | 2016-04-26 | 2018-11-27 | 福州大学 | A kind of biochip test method of induced with laser and CCD acquisition |
| CN106092995A (en) * | 2016-07-25 | 2016-11-09 | 天津水运工程勘察设计院 | A kind of indoor detection probe of fluorescent solutions concentration |
| CN106092995B (en) * | 2016-07-25 | 2019-02-12 | 天津水运工程勘察设计院 | A kind of indoor detection probe of fluorescent solutions concentration |
| CN106092994A (en) * | 2016-07-27 | 2016-11-09 | 福州大学 | A kind of micro-array chip fluorescence detection method of great power LED |
| CN106092994B (en) * | 2016-07-27 | 2019-05-10 | 福州大学 | A kind of micro-array chip fluorescence detection method of great power LED |
| CN107561043A (en) * | 2017-07-06 | 2018-01-09 | 复旦大学 | Biology sensor based on transform coding fluorescent technique on polychrome |
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| Publication number | Publication date |
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| CN103868902B (en) | 2016-03-30 |
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