CN103868902A - Bioluminescence detection probe - Google Patents

Abstract

The invention relates to a biological fluorescence detection probe, which includes a photoelectric sensor, a mounting frame for mounting a lens, and a mobile scanning platform for mounting biological reagents to be tested, and is characterized in that: the two sides of the mounting frame are symmetrical to the center line A light source is provided, and the outgoing rays of the light sources on both sides intersect on the biological reagent to be tested, and a first lens is respectively symmetrically arranged on the optical path between the light sources on both sides and the biological reagent to be tested, and the biological reagent to be tested passes through two sides. The light excited by the light irradiation of the light source then passes through a second lens and a filter installed on the installation frame to reach the photoelectric sensor in sequence. The invention has good detection effect, can replace the probe part of the existing biological fluorescence detection system to a certain extent, and has a good role in promoting the popularization and application of the biological fluorescence detection technology.

Description

A bioluminescence detection probe

technical field

The invention relates to a biological fluorescence detection probe, which belongs to the technical fields of photoelectric detection, biological fluorescence detection and analysis, and the like.

Background technique

Fluorescence assay detection is a powerful analytical tool widely used in clinical testing, biological research, agricultural science, food and environmental science. At room temperature, most molecules are in the lowest vibrational energy level of the ground state, and the molecules in the ground state absorb energy (light energy, chemical energy, electrical energy or thermal energy) and then jump to the excited state. The excited state is unstable and will soon decay to the ground state. The release of energy in the form of light is called "luminescence". Fluorescence analysis and detection technology is a form of luminescent phenomenon. Using high-energy particles to irradiate biological reagents, the fluorescent substances contained in biological reagents will be excited to emit fluorescence. The components of biological reagents are analyzed by detecting fluorescence. The fluorescence intensity is proportional to the concentration of the fluorescent substance, and certain components can be analyzed qualitatively and quantitatively through the detected fluorescence.

However, the optical path of the probe used in the existing bioluminescent detection technology is complex, which leads to bloated equipment, resulting in a large volume of the bioluminescent detection device, which cannot be carried and operated.

Contents of the invention

In view of the deficiencies in the prior art, the present invention proposes a small-volume and easy-to-operate bioluminescence detection probe through rational design and optimization of the optical path, which greatly reduces the volume and weight of the bioluminescence detection equipment and is easier for industrial promotion.

Technical scheme of the present invention is:

A biological fluorescence detection probe, including a photoelectric sensor, a mounting frame for mounting a lens, and a mobile scanning platform for mounting biological reagents to be tested, is characterized in that: a light source is symmetrically arranged on both sides of the mounting frame relative to the center line , the outgoing light rays of the light sources on both sides intersect on the biological reagent to be tested, and a first lens is respectively symmetrically arranged on the optical path between the light sources on both sides and the biological reagent to be tested, and the biological reagent to be tested is irradiated by light from the light sources on both sides The excited light passes through a second lens and a filter installed on the installation frame to reach the photoelectric sensor in sequence.

Wherein, the positions of the centers of the light sources on both sides and the positions of the biological reagents to be tested are in an isosceles right triangle relationship, and the centers of the first lenses are respectively located at the center positions of the light path distances between the centers of the corresponding side light sources and the biological reagents to be tested , the biological reagent to be tested, the photoelectric sensor and the second lens located at the same position in the middle of the two are co-located on a straight line, and the photoelectric sensor is located at the center of the light source connecting line on both sides.

The distance from the center of the light source to the center of the first lens on the corresponding side is twice the focal length of the first lens.

A diaphragm hole with an aperture of 2-2.5 mm is provided on the mounting frame located on the optical path from the light source to the first lens on the corresponding side.

The invention has the advantages of good detection effect, can replace the probe part of the existing biological fluorescence detection system to a certain extent, and has a good role in promoting the popularization and application of the biological fluorescence detection technology.

Description of drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention.

Fig. 2 is a schematic diagram of the structure of multiple isosceles right triangles according to an embodiment of the present invention.

FIG. 3 is a schematic top view of the mounting bracket according to the embodiment of the present invention.

Fig. 4 is a schematic diagram of the position and structure of the components on the mounting frame according to the embodiment of the present invention.

Detailed ways

In order to make the above-mentioned features and advantages of the present invention more comprehensible, the following specific embodiments are described in detail with reference to the accompanying drawings.

With reference to Fig. 1 to Fig. 4, the present invention relates to a kind of biological fluorescence detection probe, comprises photoelectric sensor 8, the mounting frame 7 in order to install the lens and the mobile scanning platform 10 in order to place the biological reagent 9 to be tested, described mounting frame The two sides of 7 are symmetrically provided with a light source 1 and 2 with respect to the center line, and the outgoing light rays of the light sources 1 and 2 on both sides intersect on the biological reagent 9 to be tested. A first lens 3, 4 is arranged symmetrically on the optical path respectively, and the light excited by the light source light on both sides on the biological reagent to be tested passes through a second lens 5 and a filter installed on the mounting frame 7 in turn. 6 reaches the photoelectric sensor 8.

The center positions of the above-mentioned light sources on both sides and the positions of the biological reagents to be tested are in an isosceles right triangle relationship, and the centers of the first lenses are respectively located at the center positions of the optical path distances between the centers of the corresponding side light sources and the biological reagents to be tested. The biological reagent to be tested, the photoelectric sensor and the second lens located at the same position in the middle of the two are co-located on a straight line, and the photoelectric sensor is located at the center of the connecting line of light sources on both sides.

The distance from the center of the light source to the center of the first lens on the corresponding side is twice the focal length of the first lens.

A diaphragm hole 11 with an aperture of 2-2.5 mm is provided on the mounting frame on the optical path from the light source to the first lens on the corresponding side.

Specific implementation process: the probe includes light sources 1, 2, first lenses 3, 4, second lens 5, optical filter 6, installation frame 7, sensor 8, biological reagent 9 to be tested, and mobile scanning platform 10. The dual light source structure is adopted to increase the emission power of the light source and improve the detection accuracy of the detection system. The light sources 1 and 2 are installed on the mounting frame 7 at the positions marked 1 and 2, the first lenses 3 and 4 are installed at the positions marked 3 and 4 on the mounting frame 7, and the second lens 5 is installed at the position marked 5 on the mounting frame 7 , the optical filter 6 is installed on the position marked 6 on the mounting frame 7, the mounting frame 7 is an optical component mounting frame, its size is designed according to the optical principle, and the design idea is as follows: components 1, 2, 3, 4, 5, 8, 9 form multiple isosceles right triangles as shown in Figure 2 (each triangle in the figure is an isosceles right triangle), the size of the isosceles right triangle is determined by the distance from 1 to 3 being twice the focal length of the first lens, Utilizing the symmetry properties of the isosceles right triangle, the purple light emitted by the light source at 1 and 2 is focused on the biological reagent 9 to be tested through the lenses placed at 3 and 4 to excite it to emit fluorescence, and the fluorescence is placed upward at 5 The focus of the lens at 8 is detected by the photoelectric sensor placed at 8 through the filter on it. At the same time, the aperture of the small holes on both sides is designed to be 2-2.5mm to act as a diaphragm. The mobile scanning platform 10 is used to move and position the biological reagents 9 .

The above descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope of the present invention.

Claims (4)

1. A biological fluorescence detection probe, comprising a photoelectric sensor, a mounting frame for mounting a lens and a mobile scanning platform for mounting biological reagents to be measured, characterized in that: both sides of the mounting frame are symmetrically arranged relative to the central line There is a light source, and the outgoing light rays of the light sources on both sides intersect on the biological reagent to be tested, and a first lens is respectively symmetrically arranged on the optical path between the light sources on both sides and the biological reagent to be tested, and the biological reagent to be tested passes through the light sources on both sides. The light excited by the light irradiation then passes through a second lens and a filter installed on the mounting frame to reach the photoelectric sensor in sequence. 2. A bioluminescence detection probe according to claim 1, characterized in that: the center positions of the light sources on both sides and the positions of the biological reagents to be tested are in an isosceles right triangle relationship, and the centers of the first lenses are respectively Located at the center of the light path distance between the center of the corresponding side light source and the biological reagent to be tested, the biological reagent to be tested, the photoelectric sensor, and the second lens located at an equivalent position in the middle of the two are co-located on a straight line, and the photoelectric sensor It is located in the center of the connecting lines of the light sources on both sides. 3. A biological fluorescence detection probe according to claim 1, wherein the distance from the center of the light source to the center of the first lens on the corresponding side is twice the focal length of the first lens. 4. A bioluminescence detection probe according to claim 1, characterized in that: a diaphragm hole with an aperture of 2-2.5 mm is provided on the mounting frame located on the light path from the light source to the first lens on the corresponding side.

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