CN204086274U - A kind of time-resolved fluorescence immunoassay instrument - Google Patents
A kind of time-resolved fluorescence immunoassay instrument Download PDFInfo
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- CN204086274U CN204086274U CN201420613988.4U CN201420613988U CN204086274U CN 204086274 U CN204086274 U CN 204086274U CN 201420613988 U CN201420613988 U CN 201420613988U CN 204086274 U CN204086274 U CN 204086274U
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Abstract
The utility model relates to a kind of time-resolved fluorescence immunoassay instrument, and this analyser compared with prior art improves and is, described base plate is provided with motion module, and pallet is arranged in motion module; Described motion module comprises X-axis motor, X-axis sliding sleeve, X-axis fixed bar and X-axis sliding bar, X-axis motor is connected with master control board, wherein X-axis sliding sleeve and X-axis fixed bar are slidably connected, X-axis sliding sleeve is fixedly connected with X-axis sliding bar, X-axis sliding bar is connected with tray both slid, and X-axis motor drives X-axis to slide on X-axis fixed bar and moves.The analyser that the utility model provides, when detecting, can be placed multiple reagent sample simultaneously, being controlled the movement of pallet, make the reagent sample on pallet be detected successively by master control board on pallet.So well-behaved analyzer is in the process detected, does not need to change reagent sample continually, therefore improve detection efficiency.
Description
Technical field
The utility model belongs to fluoroimmunoassay technical field, more specifically, relates to a kind of time-resolved fluorescence immunoassay instrument.
Background technology
Time-resolved fluorescence (Time-Resolved Fluorescence is called for short TRF) immunoassay is a kind of microanalysis method grown up for nearly ten years.Its principle is as tracer with trivalent rare earth ions (as Eu europium, Sm samarium, Te terbium, dy dysprosium), labelled protein, polypeptide, hormone, antibody, nucleic acid probe or biologically active cell, question response system (as: antigen-antibody immune response, biotin-labeled pentylamine reaction, nucleic acid probe hybridization reaction, target cell and effector cell kill and wound reaction etc.) occur after, time-resolved fluorescence immunoassay instrument measures the fluorescence intensity in end product, judges the concentration of measured matter in reaction system with this.
As compared to other labeling method such as chemiluminescence, radio-immunity and enzyme linked immunological, TRF has clear superiority on sensitivity, stability and multiple labelling detect, and has a extensive future.Be mainly used in the inspection item needing high sensitivity and accurate quantitative analysis at present, comprise clinical infection disease and check (hepatitis B series, HCV, HIV etc.), Endocrinological inspection (thyroid gland series TSH etc., diabetes series Insulin etc., growth hormone hGH etc., woman produces class FSH etc.), tumor marker checks (AFP, CEA etc.), science of heredity checks, peripheral blood cell counts, cytolgical examination and various scientific research project.The clinical detection project of application TRF has reached 52 kinds, and has been applied in 24 kinds of research reagents at scientific research field TRF.Along with the development of laboratory medicine, can get more and more to the mensuration of trace, ultramicron, radioactive pollution problem simultaneously can more and more be taken seriously, and therefore, time-resolved fluorescence (TRF) immunoassay has more and more wide application space.
Prior art discloses a kind of time-resolved fluorescence immunoassay instrument, this analyser comprises base plate, base plate is provided with master control system and kinematic train, kinematic train comprises chute and draw-in groove, the upper end of chute is provided with light path detection, chute is provided with positioning system, and master control system is connected with kinematic train, light path detection and positioning system.The run duration of the draw-in groove in kinematic train is controlled by master control system, so when draw-in groove moves to the end of chute, draw-in groove is fixed on the end of chute, convenient operation personnel place reagent sample, after fixing reagent sample, draw-in groove carries out testing after test card being transported to the position of specifying.
But this analyser can only detect a reagent sample at every turn, the inefficiency of detection, can not meet the requirement of people.
Utility model content
The utility model is the low defect of the time-resolved fluorescence immunoassay instrument detection efficiency that overcomes above-mentioned prior art and provide, provides the high time resolved fluoro-immunoassay of a kind of detection efficiency to draw.
For solving the problems of the technologies described above, the technical solution of the utility model is as follows:
A kind of time-resolved fluorescence immunoassay instrument, comprise shell and arrange and the movement in shell, upper light path module, lower light path module that movement comprises pallet for placing reagent sample, power module, master control board and is connected respectively with master control board, power module, power module is also connected with master control board;
When analyser runs, upper light path module sends and reagent sample that exciting light beats on pallet makes reagent sample excite send fluorescence, and lower light path module detects fluorescence;
Wherein analyser also includes motion module, and pallet is arranged in motion module; Described motion module comprises X-axis motor, X-axis sliding sleeve, X-axis fixed bar and X-axis sliding bar, X-axis motor is connected with master control board, X-axis sliding sleeve and X-axis fixed bar are slidably connected, X-axis sliding sleeve is fixedly connected with X-axis sliding bar, X-axis sliding bar is connected with tray both slid, and X-axis motor drives X-axis to slide on X-axis fixed bar and moves.
In such scheme, time-resolved fluorescence immunoassay instrument is when work, master control board control X-axis motor drives X-axis to slide on X-axis fixed bar and moves, because X-axis sliding bar and X-axis sliding sleeve, pallet link together, so sliding sleeve can drive pallet realization movement in the X-axis direction by X-axis sliding bar.Therefore multiple reagent sample can be placed on pallet, analyser is when having detected a reagent sample, master control board controlled motion module makes pallet move, next reagent sample is made to be positioned in the light path of the exciting light that light path module sends, restart upper light path module and send exciting light, can excite next reagent sample.So well-behaved analyzer is in the process detected, do not need to change reagent sample continually.
Preferably, in order to detect more reagent sample once, described motion module also includes y-axis motor, Y-axis sliding sleeve, Y-axis fixed bar and Y-axis sliding bar, y-axis motor is connected with master control board, Y-axis sliding sleeve and Y-axis fixed bar are slidably connected, Y-axis sliding sleeve is fixedly connected with Y-axis sliding bar, and Y-axis sliding bar is connected with tray both slid, and y-axis motor drives Y-axis to slide on Y-axis fixed bar and moves.
After having set up above-mentioned components and parts, X-axis motor, y-axis motor drive X-axis sliding sleeve, Y-axis sliding sleeve, and then make X-axis sliding bar, Y-axis sliding bar drive pallet to move in X-axis, Y direction.Because pallet can move in plane multiple directions, therefore can place more reagent sample on pallet, master control board controls the movement of pallet, makes the reagent sample on pallet be detected successively.
Preferably, described upper light path module comprises the luminous component, optically focused submodule and the reflective mirror that set gradually, and wherein luminous component is connected with master control board; The exciting light that luminous component sends is by penetrating on reagent sample after optically focused submodule, reflective mirror.
Preferably, optically focused submodule comprises upper light path first lens set gradually, upper light path optical filter, upper light path second lens, and the exciting light that luminous component sends is successively by being incident to reflective mirror after upper light path first lens, upper light path optical filter, upper light path second lens.
Preferably, described upper light path optical filter is 340nm optical filter.
Preferably, described lower light path module comprises the optical filtering submodule and detection sub-module that set gradually, and wherein detection sub-module is connected with master control board, and the light through reagent sample is incident in detection sub-module after photonic module after filtration.
Preferably, optical filtering submodule comprises lower light path first lens set gradually, lower light path optical filter, lower light path second lens, and the fluorescence that reagent sample inspires is by being incident to detection sub-module after lower light path first lens, lower light path optical filter, lower light path second lens.
Preferably, described lower light path optical filter is 613nm optical filter.
Preferably, in order to improve the accuracy of detection of analyser, described lower light path module also includes photomultiplier, and described photomultiplier is arranged between optical filtering submodule and detection sub-module, and photomultiplier is connected with master control board.
Preferably, described time-resolved fluorescence immunoassay instrument is provided with power light and run indicator.
Compared with prior art, the beneficial effect of technical solutions of the utility model is:
The analyser that the utility model provides, when detecting, can be placed multiple reagent sample simultaneously, being controlled the movement of pallet, make the reagent sample on pallet be detected successively by master control board on pallet.So well-behaved analyzer is in the process detected, does not need to change reagent sample continually, therefore improve detection efficiency.
Accompanying drawing explanation
The schematic appearance of the time-resolved fluorescence immunoassay instrument that Fig. 1 provides for the utility model.
The structural representation of the time-resolved fluorescence immunoassay instrument that Fig. 2 provides for the utility model.
Fig. 3 is the structural representation of lower light path module and upper light path module.
Embodiment
Accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent;
Below in conjunction with drawings and Examples, the technical solution of the utility model is described further.
Embodiment 1
As shown in Figure 1, Figure 2, Figure 3 shows, analyser comprises shell 1 and arranges and the movement in shell 1, upper light path module 5, lower light path module 6 that movement comprises pallet 2 for placing reagent sample 9, base plate 3, power module 4, master control board 7 and is connected respectively with master control board 7, power module 4, power module 4 is also connected with master control board 7.
Wherein power module 4, master control board 7, lower light path module 6 are arranged on the below of base plate 3, and upper light path module 5 is arranged on the top of base plate 3.
When analyser runs, upper light path module 5 sends and the reagent sample 9 of excitation light on pallet 2 makes reagent sample 9 excite send fluorescence, and lower light path module 6 pairs of fluorescence detect;
The base plate 3 of simultaneously analyser is provided with motion module 8, and pallet 2 is arranged in motion module 8; Motion module 8 comprises X-axis motor, X-axis sliding sleeve 81, X-axis fixed bar 82 and X-axis sliding bar 83, X-axis motor is connected with master control board 7, wherein X-axis sliding sleeve 81 and X-axis fixed bar 82 are slidably connected, X-axis sliding sleeve 81 is fixedly connected with X-axis sliding bar 83, X-axis sliding bar 83 and pallet 2 are slidably connected, and X-axis motor drives X-axis sliding sleeve 81 to move on X-axis fixed bar 82.
In such scheme, time-resolved fluorescence immunoassay instrument is when work, master control board 7 controls X-axis motor and drives X-axis sliding sleeve 81 to move on X-axis fixed bar 82, because X-axis sliding bar 83 and X-axis sliding sleeve 81, pallet 2 link together, therefore X-axis sliding sleeve 81 movement that pallet 2 can be driven to realize in the X-axis direction by X-axis sliding bar 83.
When using well-behaved analyzer to carry out testing, multiple reagent sample 9 can be placed on pallet 2, analyser is when having detected a reagent sample 9, master control board 7 can controlled motion module 8 make pallet 2 move, thus make next reagent sample 9 be positioned in the light path of the exciting light that light path module 5 sends, in startup, light path module 5 sends exciting light, can excite next reagent sample 9.In the present embodiment, in order to detect more reagent sample 9 once, motion module 8 also includes y-axis motor, Y-axis sliding sleeve 84, Y-axis fixed bar 85 and Y-axis sliding bar 86, y-axis motor is connected with master control board 7, wherein Y-axis sliding sleeve 84 and Y-axis fixed bar 85 are slidably connected, Y-axis sliding sleeve 84 is fixedly connected with Y-axis sliding bar 86, and Y-axis sliding bar 86 is connected with tray both slid 2, and y-axis motor drives Y-axis sliding sleeve 84 to move on Y-axis fixed bar 85.
After having set up above-mentioned components and parts, X-axis motor, y-axis motor drive X-axis sliding sleeve 81, Y-axis sliding sleeve 84, so make X-axis sliding bar 83, Y-axis sliding bar 86 drives pallet 2 to move in X-axis, Y direction.Therefore can place more reagent sample 9 on pallet 2, master control board 7 controls the movement of pallet 2, makes the reagent sample 9 on pallet 2 be detected successively.
In such scheme, upper light path module 5 comprises the luminous component 51, optically focused submodule and the reflective mirror 55 that set gradually, wherein luminous component 51 is connected with master control board 7, and the exciting light that luminous component 51 sends is penetrated on reagent sample 9 after optically focused submodule, reflective mirror 55.
Wherein optically focused submodule comprises upper light path first lens 52 set gradually, upper light path optical filter 53, upper light path second lens 54, and the exciting light that luminous component 51 sends is successively by being incident to reflective mirror 55 after upper light path first lens 52, upper light path optical filter 53, upper light path second lens 54.
Lower light path module 6 comprises the optical filtering submodule and detection sub-module that set gradually, and detection sub-module is connected with master control board 7, and the light through reagent sample 9 is incident in detection sub-module after photonic module after filtration.
In the present embodiment, optical filtering submodule comprises lower light path first lens 61 set gradually, lower light path optical filter 62, lower light path second lens 63, and the fluorescence that reagent sample 9 inspires is by being incident to detection sub-module after lower light path first lens 61, lower light path optical filter 62, lower light path second lens 63.
In such scheme, master control board 7 controls luminous component 51 and sends exciting light, exciting light is by penetrating on reagent sample 9 after optically focused submodule, reflective mirror 55, reagent sample 9 is excited and sends fluorescence, the fluorescence excited is incident in detection sub-module after optical filtering submodule, detection sub-module detects fluorescence, obtains testing result.
In order to improve the accuracy of detection of analyser, in the present embodiment, lower light path module 6 also includes photomultiplier, and described photomultiplier is arranged between optical filtering submodule and detection sub-module, and photomultiplier is connected with master control board 7.In the present embodiment, time-resolved fluorescence immunoassay instrument is provided with power light and run indicator.
The analyser that the utility model provides, when detecting, can be placed multiple reagent sample simultaneously, being controlled the movement of pallet, make the reagent sample on pallet be detected successively by master control board on pallet.So well-behaved analyzer is in the process detected, does not need to change reagent sample continually, therefore improve detection efficiency.
Obviously, above-described embodiment of the present utility model is only for the utility model example is clearly described, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.All do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., within the protection domain that all should be included in the utility model claim.
Claims (8)
1. a time-resolved fluorescence immunoassay instrument, comprise shell (1) and arrange and movement in shell (1), movement comprise pallet (2) for placing reagent sample (9), power module (4), master control board (7) and be connected light path module (5) respectively with master control board (7), power module (4), lower light path module (6), master control board (7) connect;
When analyser runs, upper light path module (5) sends and reagent sample (9) that exciting light beats on pallet (2) makes reagent sample (9) excite send fluorescence, and lower light path module (6) is detected fluorescence;
It is characterized in that: analyser also includes motion module (8), pallet (2) is arranged in motion module (8); Described motion module (8) comprises X-axis motor, X-axis sliding sleeve (81), X-axis fixed bar (82) and X-axis sliding bar (83), X-axis motor is connected with master control board (7), X-axis sliding sleeve (81) and X-axis fixed bar (82) are slidably connected, X-axis sliding sleeve (81) is fixedly connected with X-axis sliding bar (83), X-axis sliding bar (83) and pallet (2) are slidably connected, and X-axis motor drives X-axis sliding sleeve (81) upper mobile at X-axis fixed bar (82).
2. time-resolved fluorescence immunoassay instrument according to claim 1, it is characterized in that: described motion module (8) also includes y-axis motor, Y-axis sliding sleeve (84), Y-axis fixed bar (85) and Y-axis sliding bar (86), y-axis motor is connected with master control board (7), Y-axis sliding sleeve (84) and Y-axis fixed bar (85) are slidably connected, Y-axis sliding sleeve (84) is fixedly connected with Y-axis sliding bar (86), Y-axis sliding bar (86) and pallet (2) are slidably connected, and y-axis motor drives Y-axis sliding sleeve (84) upper mobile at Y-axis fixed bar (85).
3. time-resolved fluorescence immunoassay instrument according to claim 1 and 2, it is characterized in that: described upper light path module (5) comprises the luminous component (51), optically focused submodule and the reflective mirror (55) that set gradually, and wherein luminous component (51) is connected with master control board (7); The exciting light that luminous component (51) sends is penetrated on reagent sample after (55) by optically focused submodule, reflective mirror.
4. time-resolved fluorescence immunoassay instrument according to claim 3, it is characterized in that: optically focused submodule comprises upper light path first lens (52), upper light path optical filter (53), upper light path second lens (54) that set gradually, the exciting light that luminous component (51) sends is successively by being incident to reflective mirror (55) after upper light path first lens (52), upper light path optical filter (53), upper light path second lens (54).
5. time-resolved fluorescence immunoassay instrument according to claim 1 and 2, it is characterized in that: described lower light path module (6) comprises the optical filtering submodule and detection sub-module that set gradually, wherein detection sub-module is connected with master control board (7), and the light through reagent sample (9) is incident in detection sub-module after photonic module after filtration.
6. time-resolved fluorescence immunoassay instrument according to claim 5, it is characterized in that: optical filtering submodule comprises lower light path first lens (61), lower light path optical filter (62), lower light path second lens (63) that set gradually, the fluorescence that reagent sample (9) inspires is by being incident to detection sub-module after lower light path first lens (61), lower light path optical filter (62), lower light path second lens (63).
7. time-resolved fluorescence immunoassay instrument according to claim 5, it is characterized in that: described lower light path module (6) also includes photomultiplier, described photomultiplier is arranged between optical filtering submodule and detection sub-module, and photomultiplier is connected with master control board (7).
8. time-resolved fluorescence immunoassay instrument according to claim 1, is characterized in that: described time-resolved fluorescence immunoassay instrument is provided with power light and run indicator.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111060493A (en) * | 2019-12-28 | 2020-04-24 | 安徽中科赛飞尔科技有限公司 | Surface-enhanced Raman spectrum multi-sample detection system |
| CN111537707A (en) * | 2020-05-28 | 2020-08-14 | 上海邦先医疗科技有限公司 | Chemiluminescence immunoassay analyzer, chemiluminescence immunoassay system and chemiluminescence immunoassay detection method |
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2014
- 2014-10-22 CN CN201420613988.4U patent/CN204086274U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111060493A (en) * | 2019-12-28 | 2020-04-24 | 安徽中科赛飞尔科技有限公司 | Surface-enhanced Raman spectrum multi-sample detection system |
| CN111537707A (en) * | 2020-05-28 | 2020-08-14 | 上海邦先医疗科技有限公司 | Chemiluminescence immunoassay analyzer, chemiluminescence immunoassay system and chemiluminescence immunoassay detection method |
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