CN111855508A - Liquid detection device and liquid detection method - Google Patents
Liquid detection device and liquid detection method Download PDFInfo
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- 239000007788 liquid Substances 0.000 title claims abstract description 181
- 238000001514 detection method Methods 0.000 title claims abstract description 140
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- 238000000034 method Methods 0.000 abstract description 35
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- 238000010521 absorption reaction Methods 0.000 description 4
- 230000031700 light absorption Effects 0.000 description 4
- 230000001360 synchronised effect Effects 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
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- 238000005259 measurement Methods 0.000 description 2
- 238000001208 nuclear magnetic resonance pulse sequence Methods 0.000 description 2
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- 238000013461 design Methods 0.000 description 1
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- 239000012530 fluid Substances 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
A liquid detection apparatus and method, the liquid detection apparatus comprising: a pulsed light source configured to emit pulsed light; the time domain dispersion device is configured to convert the pulse light emitted by the pulse light source from a frequency domain to a time domain to form pulse light with a broadened time domain; the first objective lens is configured to converge the pulsed light of which the propagation direction is changed by the first spatial dispersion device to the microfluidic device; a microfluidic device configured to cause a liquid to be detected therein to flow at a predetermined speed; the second objective lens is configured to collect the light collected by the first objective lens after passing through the microfluidic device; the second spatial dispersion device is used for spatially recombining the light collected by the second objective lens; and the photoelectric detection device is configured to image the light recombined by the second spatial dispersion device so as to detect the particles in the liquid.
Description
Technical Field
The disclosed embodiments relate to a liquid detection apparatus and a liquid detection method.
Background
A liquid detection device, such as a blood analyzer for detecting a blood sample, a water quality analyzer for detecting river water quality, or the like, is an apparatus for qualitatively and quantitatively analyzing a visible component, such as a particle, in a liquid and providing related information. The automatic liquid analyzer can be classified into a resistance type or a comprehensive application type using a plurality of high and new technologies according to the working principle, and the number and rough classification of cells or other particles in the liquid are obtained by processing sampling data through a computer.
The liquid analyzing apparatus described above has a low liquid analyzing speed, but is expensive and has been difficult to be used in conventional detection.
Disclosure of Invention
The present disclosure provides a liquid detection apparatus and a liquid detection method to solve the above technical problems.
According to at least one embodiment of the present disclosure, there is provided a liquid detection apparatus including: the system comprises a pulse light source, a time domain dispersion device, a first spatial dispersion device, a first objective lens, a microflow device, a second objective lens, a second spatial dispersion device and a photoelectric detection device, wherein the pulse light source is configured to emit pulse light; the time domain dispersion device is configured to convert the pulse light emitted by the pulse light source from a frequency domain to a time domain to form pulse light with a broadened time domain; the first spatial dispersion device is configured to change the propagation direction of light with different frequencies in the pulsed light after the time domain broadening in the space, and the first objective lens is configured to converge the pulsed light of which the propagation direction is changed by the first spatial dispersion device to the microfluidic device; the microfluidic device is configured to enable the liquid to be detected to flow at a preset speed; the second objective lens is configured to collect the light collected by the first objective lens and passing through the microfluidic device; the second spatial dispersion device is used for spatially recombining the light collected by the second objective lens; the photoelectric detection device is configured to image the light recombined by the second spatial dispersion device so as to detect the particles in the liquid.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, wherein the pulsed light source comprises an infrared pulsed light source.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes an amplifier, located between the time-domain dispersing device and the first spatial dispersing device, for amplifying the time-domain broadened pulsed light and transmitting the amplified pulsed light to the first spatial dispersing device.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, further comprises a first beam splitter located between the second objective and the second spatial dispersion device; the light collected by the second objective lens and passing through the microfluidic device is transmitted through the first beam splitter and enters the second spatial dispersion device.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, further comprises a first continuous wave laser configured to emit continuous visible light and to inject the continuous visible light to the second objective lens; the continuous visible light passing through the second objective lens is converged to at least one part of the microfluidic device to irradiate the liquid in the part of the microfluidic device, so that the particles in the liquid are excited to generate light.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes a first beam splitter, to which the continuous visible light emitted by the first continuous wave laser is incident; the continuous visible light passing through the first beam splitter is reflected to the second objective.
The device according to any of the preceding embodiments of the present disclosure, for example, further comprises a first particle detection device, wherein light generated by particles in the liquid is incident on the first particle detection device, and the first particle detection device detects the generated light.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes a first band-pass filter, wherein light generated by particles in the liquid is incident on the first band-pass filter, and light filtered by the first band-pass filter is incident on the first particle detection apparatus.
The device according to any of the previous embodiments of the present disclosure, for example, further comprises a first beam splitter, wherein light generated by particles in the liquid in the microfluidic device is incident on the first beam splitter through the second objective; the first beam splitter reflects light generated by particles in the liquid to the first band pass filter.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes a first beam splitter located between the first beam splitter and the first band pass filter, where light reflected by the first beam splitter passes through the first beam splitter, and light with a first parameter at a predetermined threshold in the reflected light is transmitted through the first beam splitter and enters the first band pass filter.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes a plane mirror, and the continuous visible light emitted by the continuous wave laser is transmitted to the first spectroscope through the plane mirror.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes at least one second continuous light laser that emits visible light in a different wavelength range from the first continuous light laser, light of the first continuous light laser and light of the second continuous light laser being incident on the second objective lens; the light passing through the second objective lens is focused on at least one part of the microfluidic device to irradiate the liquid in the part of the microfluidic device, so that particles in the liquid are excited to generate light.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes at least one second beam splitter that combines the light of the first continuous light laser and the at least one second continuous light laser into one beam of light and inputs the one beam of light to the second objective lens.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, further comprises at least one second particle detection device, each of the continuous light lasers corresponding to one of the particle detection devices, each of the particle detection devices detecting light generated by liquid particles in the microfluidic device excited by the corresponding continuous light laser.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further comprises at least one second band-pass filter, each of the first or second band-pass filters corresponds to one of the particle detection devices, and light passing through the band-pass filter is incident on the corresponding particle detection device, so that each of the particle detection devices respectively detects the liquid.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, the filter wavelength parameters between any two of the first band pass filter and the at least one second band pass filter are different.
The apparatus according to any of the foregoing embodiments of the present disclosure, for example, further includes at least one third beam splitter, where the light reflected by the first beam splitter is incident to the at least one third beam splitter, and then is incident to the first band pass filter or the second band pass filter through the at least one third beam splitter, and each third beam splitter corresponds to one band pass filter.
According to the apparatus of any of the preceding embodiments of the present disclosure, for example, the first continuous wave laser and the second continuous wave laser emit laser light simultaneously.
According to the device of any one of the preceding embodiments of the present disclosure, for example, the pulse laser emits pulses at the same frequency as the frame rate of the photodetector.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, wherein the first spatially dispersive device is a diffraction grating.
According to the device of any one of the preceding embodiments of the present disclosure, for example, the numerical apertures of the first objective lens and the second objective lens are the same.
According to the device of any one of the preceding embodiments of the present disclosure, for example, the magnification parameters of the first objective lens and the second objective lens are the same.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, the first and second spatial dispersive devices have the same parameters.
In a device according to any of the preceding embodiments of the present disclosure, for example, the light generated by particles in the liquid comprises light generated by liquid particles in a cross-section of the microfluidic device in a direction perpendicular to the flow direction.
The device according to any of the preceding embodiments of the present disclosure, for example, wherein the liquid comprises a body fluid, and wherein the absorption parameter of the particles in the liquid is proportional to the hemoglobin content of the body fluid.
According to the device of any one of the previous embodiments of the present disclosure, for example, the continuous wave laser is incident on a first focus of the microfluidic device at the same position as a second focus of the microfluidic device on which light emitted from the pulsed light source is incident.
The apparatus according to any of the preceding embodiments of the present disclosure, for example, further comprises a signal conditioning device that conditions the signal on the photodetection device and the signal on the particle detection device such that the two signals are synchronized in time.
There is also provided, in accordance with at least one embodiment of the present disclosure, a liquid detection method, including: the pulse light source emits pulse light; the time domain dispersion device converts the pulse light emitted by the pulse light source from a frequency domain to a time domain to form pulse light with a broadened time domain; the first spatial dispersion device changes the propagation direction of light with different frequencies in the pulse light after the time domain broadening in the space, and the first objective lens converges the pulse light of which the propagation direction is changed by the first spatial dispersion device to the microfluidic device; the microfluidic device enables the liquid to be detected to flow at a preset speed; the second objective lens converges the first objective lens and collects the light passing through the microfluidic device; the second spatial dispersion device spatially recombines the light collected by the second objective lens; and the photoelectric detection device images the light recombined by the second spatial dispersion device so as to detect the particles in the liquid.
According to the method of any of the preceding embodiments of the present disclosure, for example, the pulsed light source emits infrared pulsed light.
The method according to any of the previous embodiments of the present disclosure, for example, further comprises amplifying the temporally broadened pulsed light using an amplifier located between the temporal dispersion device and the first spatial dispersion device, and transmitting the amplified pulsed light to the first spatial dispersion device.
The method according to any of the preceding embodiments of the present disclosure, for example, further comprising positioning a first beam splitter between the second objective and the second spatial dispersion device; the light collected by the second objective lens and passing through the microfluidic device is transmitted through the first beam splitter and enters the second spatial dispersion device.
The method according to any of the foregoing embodiments of the present disclosure, for example, further includes that the continuous visible light emitted by the first continuous wave laser is incident on the second objective lens; the continuous visible light passing through the second objective lens is converged to at least one part of the microfluidic device to irradiate the liquid in the part of the microfluidic device, so that the particles in the liquid are excited to generate light.
According to the method of any one of the preceding embodiments of the present disclosure, for example, the continuous visible light emitted by the first continuous wave laser is incident on the first beam splitter; the continuous visible light passing through the first beam splitter is reflected to the second objective.
The method according to any of the preceding embodiments of the present disclosure, for example, further comprising that light generated by particles in the liquid is incident on a first particle detection device, which detects the generated light.
According to the method of any of the preceding embodiments of the present disclosure, for example, light generated by particles in the liquid is incident on a first band-pass filter, and light filtered by the first band-pass filter is incident on the first particle detection device.
According to the method of any of the previous embodiments of the present disclosure, for example, light generated by particles in the liquid of the microfluidic device is incident on the first beam splitter through the second objective lens; the first beam splitter reflects light generated by particles in the liquid to the first band pass filter.
The method according to any of the foregoing embodiments of the present disclosure, for example, further includes providing a first beam splitter located between the first beam splitter and the first band pass filter, wherein light reflected by the first beam splitter passes through the first beam splitter, and light with a first parameter at a predetermined threshold in the reflected light is transmitted through the first beam splitter and enters the first band pass filter.
According to the method of any one of the preceding embodiments of the present disclosure, for example, the continuous visible light emitted by the continuous wave laser is transmitted to the first beam splitter via a plane mirror.
The method according to any of the foregoing embodiments of the present disclosure, for example, further includes providing at least one second continuous light laser that emits visible light in a different wavelength range from the first continuous light laser, wherein light of the first continuous light laser and light of the second continuous light laser are incident on the second objective lens; the light passing through the second objective lens is focused on at least one part of the microfluidic device to irradiate the liquid in the part of the microfluidic device, so that particles in the liquid are excited to generate light.
According to the method of any of the foregoing embodiments of the present disclosure, for example, at least one second beam splitter combines the light of the first continuous light laser and the light of the at least one second continuous light laser into a beam of light, and the beam of light is incident on the second objective.
The method according to any of the preceding embodiments of the present disclosure, for example, further comprising providing at least one second particle detection device, each of the continuous light lasers corresponding to one of the second particle detection devices, wherein each of the particle detection devices detects light generated by liquid particles in the microfluidic device excited by the corresponding continuous light laser.
The method according to any of the foregoing embodiments of the present disclosure, for example, further comprises providing at least one second band-pass filter, each of the first or second band-pass filters corresponding to one of the particle detection devices, wherein light passing through the band-pass filter is incident on the corresponding particle detection device, so that each of the particle detection devices respectively detects the liquid.
The method according to any of the preceding embodiments of the present disclosure, for example, wherein a filtering wavelength parameter is different between any two of the first band pass filter and the at least one second band pass filter.
According to the method of any of the preceding embodiments of the present disclosure, for example, the light reflected by the first beam splitter is incident to at least one third beam splitter, and is incident to the first band pass filter or the second band pass filter through the at least one third beam splitter, where each third beam splitter corresponds to one band pass filter.
According to the method of any of the preceding embodiments of the present disclosure, for example, the first continuous wave laser and the second continuous wave laser emit laser light simultaneously.
According to the method of any of the preceding embodiments of the present disclosure, for example, the pulse laser emits pulses at the same frequency as the frame rate of the photodetector.
The method according to any of the preceding embodiments of the present disclosure, for example, the first spatially dispersive device is a diffraction grating.
According to the method of any of the preceding embodiments of the present disclosure, for example, the numerical apertures of the first objective lens and the second objective lens are the same.
According to the method of any of the preceding embodiments of the present disclosure, for example, the magnification parameters of the first objective lens and the second objective lens are the same.
According to the method of any of the preceding embodiments of the present disclosure, for example, the parameters of the first spatial dispersive device and the second spatial dispersive device are the same.
The method according to any of the preceding embodiments of the present disclosure, for example, wherein the light generated by particles in the liquid comprises light generated by liquid particles in a cross-section of the microfluidic device in a direction perpendicular to the flow direction.
The method according to any of the preceding embodiments of the present disclosure, for example, wherein the liquid comprises a body fluid, and the light absorption parameter of the particles in the liquid is proportional to the hemoglobin content of the body fluid.
According to the method of any of the previous embodiments of the present disclosure, for example, the continuous wave laser is incident on a first focus of the microfluidic device at the same position as a second focus of the microfluidic device where the light emitted from the pulsed light source is incident.
The method according to any of the preceding embodiments of the present disclosure, for example, further comprising adjusting the signal on the photodetection device and the signal on the particle detection device by a signal adjusting device such that the two signals are synchronized in time.
The liquid detection device and the liquid detection method of the embodiment of the disclosure improve the detection speed due to the imaging by using the optical device, and are helpful for distinguishing the types and the numbers of the particles in the liquid by detecting the shapes of the particles in the liquid.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings used in the description of the embodiments will be briefly introduced below. The drawings in the following description are merely exemplary embodiments of the disclosure.
FIG. 1 illustrates a schematic diagram of a liquid detection device according to an embodiment of the present disclosure;
FIG. 2 illustrates another schematic structural diagram of a liquid detection device according to an embodiment of the present disclosure;
FIG. 3 shows a schematic view of another configuration of a liquid detection device according to an embodiment of the present disclosure;
FIG. 4 shows a pulse sequence schematic of a photodetector measurement according to an embodiment of the present disclosure;
FIG. 5 illustrates a liquid detection method according to an embodiment of the present disclosure.
Detailed Description
Hereinafter, preferred embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. Note that in the present specification and the drawings, steps and elements having substantially the same structure are denoted by the same reference numerals, and repeated explanation of the steps and elements will be omitted.
Fig. 1 shows a schematic structural diagram of a liquid detection device according to an embodiment of the present disclosure. A liquid detection apparatus according to an embodiment of the present disclosure will be described below with reference to fig. 1. Referring to fig. 1, the liquid detection apparatus 100 includes a pulsed light source 110, a time domain dispersion device 120, a first spatial dispersion device 130, a first objective lens 140, a microfluidic device 150, a second objective lens 160, a second spatial dispersion device 170, and a photodetection apparatus 180.
A pulsed light source 110 configured to emit pulsed light. The pulsed light source comprises an infrared pulsed light source. The pulsed light source 110 may, for example, emit periodic short-pulse laser light in a wavelength band of, for example, invisible light, such as infrared. The laser light emitted by the pulsed light source 110 may be repeated at a certain frequency.
And the time domain dispersion device 120 is configured to convert the pulsed light emitted by the pulsed light source from a frequency domain to a time domain to form the pulsed light with a broadened time domain. The time domain dispersive device may be, for example, a dispersive optical fiber, a fiber grating, a grating, or the like.
A first spatial dispersion device 130 configured to change a propagation direction of light of different frequencies in the temporally broadened pulsed light in space. The first spatial dispersive device is a diffraction grating. The mapping in space is achieved for the pulsed light that was temporally broadened by the previous time-domain dispersive device 120.
And a first objective lens 140 configured to converge the pulsed light whose propagation direction is changed by the first spatial dispersion device 130 to the microfluidic device 150. The first objective 140 is, for example, a combination of optical elements, which focuses the light on the microfluidic device or on a part of the microfluidic device for illuminating the liquid in the part of the microfluidic device. For example, if the focal point is located on a cross section in a direction perpendicular to the flow direction in the microfluidic device 150, or a space in a direction perpendicular to the flow direction, particles in the liquid on the cross section or the space are irradiated with the pulsed light.
A microfluidic device 150 configured to cause a liquid to be detected therein to flow at a predetermined speed. The micro-fluidic device is, for example, a micro-fluidic chip, a capillary tube, etc., and the flow rate can be set to microliter/second to milliliter/second according to actual needs. The liquid in the microfluidic device flows at a certain speed. Under irradiation of invisible light such as pulsed light, generally, the liquid particles in the microfluidic device 150 do not absorb the pulsed light. Under the irradiation of visible light, the particles in the liquid in the microfluidic device 150 may absorb a portion of the visible light, and be excited by the visible light to emit light. In the detection, the detection may be performed in units of a cross section of the microfluidic device 150, for example, the cross section may be a cross section or a section of a space perpendicular to the flow direction, or may be a cross section or a space in other directions of the microfluidic device.
The microfluidic device 150 may store any fluid to be detected, such as body fluid, river water, and industrial water. The change in absorption (e.g., absorbance) of particulate matter in a liquid is proportional to the amount of particles in the body fluid, e.g., the amount of light absorption of particulates in blood is proportional to the amount of hemoglobin. In addition, the type, amount, and density of the photosensitive molecules in the particles in the liquid are also related to the above-mentioned absorbance change.
And a second objective lens 160 configured to collect light collected by the first objective lens after passing through the microfluidic device. The second objective lens 160 may be, for example, the same or different optical element or combination of optical elements as the first objective lens 140. In one example, the magnification parameters of the first objective lens and the second objective lens are the same. In another example, the numerical apertures of the first objective lens and the second objective lens are the same. The physical parameters of the first objective lens and the second objective lens may also be identical.
The second spatial dispersion device 170 spatially recombines the light collected by the second objective lens 160. The physical parameters of the first and second spatial dispersion devices may be the same.
And a photodetection device 180 configured to image the light recombined by the second spatial dispersion device to detect the particles in the liquid, e.g., determine the shape of the particles in the liquid, by the imaging image. FIG. 4 shows a pulse sequence schematic of a photodetector measurement according to an embodiment of the present disclosure. Fig. 4 is a spectrum obtained by detecting flowing particles on a one-dimensional cross section perpendicular to the flowing direction in a microfluidic device, and the spectrum can be a pulse spectrum, wherein the abscissa represents time, and the ordinate represents the amplitude value of a light pulse, namely the spectrum change obtained by the flowing particles on the cross section changing along with time. The shape of the particle can be detected from the change in the waveform of the spectrum.
In one example, the pulse laser 110 emits pulses at the same frequency as the frame rate of the photodetectors in order to have each pulse correspond to each frame of imaging. That is, the number of pictures taken by the photodetector per second is the same as the frequency of the pulses emitted by the pulsed laser 110.
The liquid detection device of the embodiment of the disclosure improves the detection speed by using the optical device for imaging, and is helpful for distinguishing the type and the number of the particles in the liquid by detecting the shape of the particles in the liquid.
The liquid detection apparatus according to the embodiments of the present disclosure is described above, and another liquid detection apparatus according to the embodiments of the present disclosure is described below based on the embodiments, the liquid detection apparatus is implemented on the basis of the embodiments, and any example or example combination of the liquid detection apparatuses may be applied to the embodiments. And will not be described in detail herein.
Fig. 2 shows another liquid detection apparatus 200 according to an embodiment of the present disclosure, and another liquid detection apparatus according to an embodiment of the present disclosure will be described below with reference to fig. 2, which includes the following components in addition to the components of the liquid detection apparatus of the foregoing embodiment.
Referring to fig. 2, in an example, in order to amplify the time-domain broadened pulsed light and improve the signal intensity, the liquid detection apparatus 200 may further include an amplifier (not shown in the figure). The amplifier may be located between the time domain dispersing device 120 and the first spatial dispersing device 130, and is configured to amplify the pulsed light after the time domain is broadened by the time domain dispersing device 120, and transmit the amplified pulsed light to the first spatial dispersing device.
In one example, the liquid detection apparatus 200 may further include a first spectroscope 220. The first beam splitter 220 can transmit or reflect light according to the wavelength of the light, for example, light of a specific wavelength can be incident and pass through the first beam splitter 220, and light of other wavelengths can be reflected by the first beam splitter. For example, light in the invisible light band may be transmitted through the dichroic beamsplitter, while light in the visible light band is reflected by the dichroic beamsplitter. The first beam splitter 220 is, for example, a dichroic beam splitter. The first beam splitter 220 may be located between the second objective lens 160 and the second spatial dispersion device 170. The invisible light emitted from the pulsed light source 110 may be transmitted through the first beam splitter to enter the second spatial dispersion device after being collected by the second objective lens and passing through the microfluidic device.
According to an embodiment of the present disclosure, referring to fig. 2, the liquid detection apparatus 200 may further include a first continuous wave laser 210 that may emit continuous visible light, and the continuous visible light generated by the first continuous wave laser 210 may be incident on a second objective lens in order to irradiate the visible light to the liquid and excite particles in the liquid to generate fluorescence. The continuous visible light passing through the second objective lens is converged on the microfluidic device to irradiate the liquid in the microfluidic device, so that particles in the liquid are excited to generate light. Alternatively, the continuous visible light is focused onto at least a portion of the microfluidic device to illuminate the liquid in the portion of the microfluidic device to excite particles in the liquid to produce light.
In another example, the continuous visible light emitted from the first continuous wave laser 210 is first incident on the first beam splitter 220, and the visible light is reflected by the first beam splitter 220 to the second objective. In this way, the visible light reflected to the second objective lens is focused onto the microfluidic device through the second objective lens to irradiate the liquid in the microfluidic device, so as to excite the particles in the liquid to generate light. Alternatively, the continuous visible light reflected to the second objective is focused by the second objective onto at least a portion of the microfluidic device to illuminate the liquid in the portion of the microfluidic device, thereby exciting particles in the liquid to produce light.
In one example, the first continuous wave laser 210 is incident on a first focal point of the microfluidic device at the same location as a second focal point of the pulsed light source, so that shape detection and other property monitoring of particles in the liquid at the location can be performed simultaneously.
In one example, the liquid detection device 200 may further include a first particle detection device 240, see the path indicated by the dashed arrow in fig. 2. The first particle detection device 240 is used to detect light generated by the liquid in the microfluidic device, for example, the first particle detection device 240 can detect light with certain wavelength, and since the light with the certain wavelength is emitted by the particles in the liquid to be detected, the characteristics of the particles in the liquid corresponding to the light can be known according to the detection result. For example, the properties of the particles may be determined based on the parameters of the emitted fluorescent light having different wavelengths and/or directions.
Referring to fig. 2, referring to the path indicated by the dashed arrow in fig. 2, in one example, the liquid detection device 200 may further include a first band pass filter 230. The bandpass filter may pass light of a particular wavelength while light of other wavelengths is filtered out. Light generated by the liquid in the microfluidic device is firstly incident on the first band-pass filter 230, so that light with a specific wavelength passes through the first band-pass filter 230, the light passing through the first band-pass filter 230 is further incident on the first particle detection device 240, so that the first particle detection device 240 detects the light with the specific wavelength, and according to a detection result, characteristics of particles in the liquid corresponding to the part of light are obtained.
In addition, in one example, referring to the path indicated by the dotted arrow in fig. 2, the light generated by the liquid particles in the microfluidic device may pass through the second objective lens 160 and then enter the first beam splitter 220, and the first beam splitter 220 further reflects the light generated by the liquid particles. That is, the light passing through the first beam splitter 220, wherein the light in the invisible wavelength band is transmitted through the first beam splitter and incident to the second spatial dispersion device. The light in the visible light band cannot pass through the first beam splitter 220 and is reflected by the first beam splitter 220.
Referring to fig. 2, in one example, the liquid detection device 200 may further include a first band pass filter 230 and a first particle detection device 240. The bandpass filter may pass light of a particular wavelength while light of other wavelengths is filtered out. The light reflected by the first beam splitter 220 enters the first band pass filter 230, so that the light with a specific wavelength passes through the first band pass filter 230, and the light passing through the first band pass filter 230 further enters the first particle detection device 240, so that the first particle detection device 240 only detects the light with the specific wavelength passing through the first band pass filter 230, and the light with the specific wavelength represents the absorption of the light with a certain wavelength band by the particles in the liquid, so that the characteristics of the particles in the liquid corresponding to the light can be known according to the detection result.
In one example, the liquid detection device 200 may further include a signal adjustment device 270, and the signal adjustment device 270 may adjust the signal on the photodetector 180 and the signal on the first particle detection device 240 such that the two signals are synchronized in time. By synchronizing the signals, the intensities of the two optical signals at the same time point can be detected.
In one example, referring to fig. 2, in the liquid detection apparatus 200, a first beam splitter 250 may be further included. The first beam splitter may be transmissive to light of a particular wavelength and opaque to light of other wavelengths. The first beam splitter 250 may be located between the first beam splitter 220 and the first band pass filter 230. In this way, after the light reflected by the first beam splitter 220 passes through the first beam splitter 250, the light having a specific wavelength or a specific frequency among the reflected light is transmitted to the first particle detection device 240. Or through the first band pass filter 230 to the first particle detection device 240. For example, a threshold value of wavelength or frequency may be preset, and only light having a wavelength or frequency at the predetermined threshold value is transmitted through the first beam splitter 250.
For example, the light reflected from the first beam splitter 220 includes light generated by particles in the liquid, and may also include light reflected by the first continuous light laser projected to the microfluidic device and reflected by the microfluidic device or the liquid in the microfluidic device, and since the first particle detection device 240 only detects light generated by particles, the first beam splitter 250 may block light except light generated by particles, so that the detected light is free from noise, and the detection accuracy is improved.
In addition, in one example, the liquid detection apparatus 200 may further include one or more mirrors or lenses or other optical elements for adjusting the optical path such that the light is irradiated in a predetermined direction. For example, a plane mirror (not shown) may be located between the continuous wave laser 210 and the first beam splitter 220, and the continuous visible light emitted from the continuous wave laser 210 may be incident on the first beam splitter 220 via the plane mirror. Alternatively, a plane mirror may be located between the continuous wave laser 210 and the first beam splitter 250, and the continuous visible light emitted from the continuous wave laser 210 may be incident on the first beam splitter 250 through the plane mirror.
In the above, the liquid detection apparatus according to another embodiment of the present disclosure is introduced, in this embodiment, the liquid detection apparatus can achieve the functions of adjusting, splitting, filtering, and the like of the optical path through the plurality of auxiliary elements, so that the detection of the light generated by the particles in the liquid can be realized, the light absorption property of the particles in the liquid can be further known according to the detection of the generated light, and the characteristics of the particles, such as color, number, size, and the like, can be determined according to the light absorption property.
Yet another liquid detection device according to embodiments of the present disclosure is further described below. There are often multiple particles in a liquid, and each particle may also have multiple properties, so as to improve the detection speed by detecting multiple particles simultaneously or detecting multiple properties of one particle simultaneously, fig. 3 shows a third liquid detection device according to an embodiment of the disclosure. The third liquid detection device is an improvement based on the first liquid detection device and/or the second liquid detection device, and any one of the embodiments, the combination of the embodiments, and any one of the examples or the combination of the examples can be used in the third liquid detection device. The liquid detection apparatus 300 in fig. 3 is re-drawn only with respect to the first beam splitter, the first continuous wave laser, the first band pass filter, and the first particle detection apparatus in the liquid detection apparatus 100 or the liquid detection apparatus 200 in the foregoing embodiment, and other components and structures are the same as those in the liquid detection apparatus 100 or the liquid detection apparatus 200 described above. And will not be described in detail herein.
Referring to fig. 3, in the liquid detection apparatus 300, at least one second continuous light laser 310 is included. Such as a second continuous laser or a plurality of second continuous lasers. The wavelength range of the visible light emitted by second continuous wave laser 310 is different from that of first continuous wave laser 210, so that different continuous wave lasers can emit visible light in different wavelength ranges, thereby exciting particles in the liquid to generate different light and detecting different properties of the particles. Of course, different particles in the liquid react differently to different wavelengths of light, and therefore, irradiation with visible light of multiple wavelengths can excite different particles in the liquid to generate different light, so that different particles present in the liquid and different properties of the particles can be effectively detected.
Further, the first continuous wave laser 210 and the second continuous light laser 310 may emit laser light simultaneously. The liquid detection device 300 may further include at least one second beam splitter 320 that combines the light from the first continuous wave laser 210 and the second continuous wave laser 310 into a beam and directs the beam to the second objective such that the beam of light passing through the second objective is focused onto at least a portion of the microfluidic device to illuminate the liquid in the portion of the microfluidic device to excite particles in the liquid to produce light.
In one example, the liquid detection device 300 further includes at least one third spectroscope 330, the third spectroscope 330 being located between the first beam splitter 250 and the first band pass filter 230 or between the first beam splitter 250 and the first particle detection device 240. Thus, the light reflected by the first beam splitter 220 is incident on the at least one third beam splitter 330 through the first beam splitter 250, and is incident on the first particle detection device 240 or the first band pass filter through the at least one third beam splitter 330.
In one example, the liquid detection apparatus 300 further comprises at least one second band-pass filter 340. Thus, the light passing through the third beam splitter 330 may be incident on the first band pass filter and one or more second band pass filters, and each of the third beam splitters may correspond to one of the first band pass filter and the second band pass filter. Through the plurality of band-pass filters, the light of the third beam splitter 330 can be filtered at different wavelengths.
In one example, the filter wavelength parameters are different between any two of the first and second bandpass filters. Therefore, light with different wavelengths can pass through different band-pass filters, and the light with various wavelengths is shunted.
In one example, the liquid detection apparatus 300 may further include at least one second particle detection apparatus 350, each of the first or second band pass filters corresponding to one of the first or second particle detection apparatuses, such that light passing through one band pass filter is incident to the corresponding liquid detection apparatus to cause each liquid detection apparatus to detect light of a corresponding wavelength, respectively, thereby simultaneously detecting various indexes of particles in the liquid.
In the liquid detection apparatus of the embodiments of the present disclosure, one or more mirrors or lenses or other optical elements may be provided to adjust the optical path for transmission needs of the optical path, as will be understood by those skilled in the art.
According to the embodiment of the disclosure, by arranging the plurality of continuous wave lasers and the plurality of liquid detection devices, particles in liquid can be imaged, and fluorescence generated by the particles in the liquid aiming at visible light with different wavelengths can be detected, so that different attribute indexes of one or more particles in the liquid can be detected simultaneously.
In addition, the disclosure also provides a liquid detection method. The liquid detection method corresponds to any one of the embodiments and any one of the examples, and the method can be applied to any one of the embodiments or examples. For the sake of brevity of the description, only a brief description will be made below.
FIG. 5 illustrates a liquid detection method according to an embodiment of the present disclosure. Referring to fig. 5, a liquid detection method 500 may include the following steps.
S501, emitting pulsed light by a pulse light source;
s502, the time domain dispersion device converts the pulse light emitted by the pulse light source from a frequency domain to a time domain to form pulse light with a broadened time domain;
s503, the first spatial dispersion device changes the propagation direction of light with different frequencies in the pulse light after time domain broadening in the space;
s504, the first objective lens converges the pulse light of which the propagation direction is changed by the first spatial dispersion device to the microfluidic device;
s505, enabling the liquid to be detected in the microfluidic device to flow at a preset speed;
s506, the second objective lens collects the light which is converged by the first objective lens and passes through the microfluidic device;
s507, the second spatial dispersion device recombines the light collected by the second objective lens on the space;
and S508, imaging the light recombined by the second spatial dispersion device by the photoelectric detection device so as to detect the particles in the liquid.
For example, the pulsed light source emits infrared pulsed light.
For example, the method further includes amplifying the time-domain broadened pulsed light using an amplifier located between the time-domain dispersing device and the first spatial dispersing device, and transmitting the amplified time-domain broadened pulsed light to the first spatial dispersing device.
For example, further comprising positioning a first beam splitter between the second objective and the second spatial dispersion device; the light collected by the second objective lens and passing through the microfluidic device is transmitted through the first spectroscope and enters the second spatial dispersion device.
For example, the method further includes that the continuous visible light emitted by the first continuous wave laser is incident to the second objective lens; the continuous visible light passing through the second objective lens converges to at least a portion of the microfluidic device to illuminate the liquid within the portion of the microfluidic device, thereby exciting particles in the liquid to produce light.
For example, the continuous visible light emitted from the first continuous wave laser is incident on the first beam splitter; the continuous visible light passing through the first beam splitter is reflected to the second objective.
For example, the method further comprises that light generated by particles in the liquid is incident on the first particle detection device, and the first particle detection device detects the generated light.
For example, light generated by particles in the liquid is incident on a first band-pass filter, and light filtered by the first band-pass filter is incident on a first particle detection device.
For example, light generated by particles in the liquid of the microfluidic device is incident on the first beam splitter through the second objective lens; the first beam splitter reflects light generated by particles in the liquid to the first band pass filter.
For example, the method further includes disposing a first beam splitter between the first beam splitter and the first band pass filter, wherein light reflected by the first beam splitter passes through the first beam splitter, and light with a first parameter at a predetermined threshold in the reflected light is transmitted through the first beam splitter and enters the first band pass filter.
For example, the continuous visible light emitted from the continuous wave laser is transmitted to the first spectroscope via the plane mirror.
For example, the method further comprises the steps of providing at least one second continuous light laser, wherein the wavelength range of visible light emitted by the second continuous light laser is different from that of the first continuous light laser, and light of the first continuous light laser and light of the second continuous light laser are incident to the second objective lens; the light passing through the second objective lens is focused onto at least a portion of the microfluidic device to irradiate the liquid in the portion of the microfluidic device, thereby exciting particles in the liquid to produce light.
For example, the at least one second spectroscope combines the light of the first continuous light laser and the light of the at least one second continuous light laser into one beam of light, and the one beam of light is incident to the second objective lens.
For example, the method may further comprise providing at least one second particle detection device, each continuous light laser corresponding to one of the particle detection devices, wherein each particle detection device detects light generated by liquid particles in the microfluidic device excited by the corresponding continuous light laser.
For example, the method further comprises providing at least one second band-pass filter, each of the first or second band-pass filters corresponding to one of the particle detection devices, wherein light passing through the band-pass filter is incident on the corresponding particle detection device, so that each particle detection device detects the liquid separately.
For example, the filter wavelength parameters are different between any two of the first bandpass filter and the at least one second bandpass filter.
For example, the light reflected by the first beam splitter is incident on at least one third beam splitter, and then is incident on the first band pass filter or the second band pass filter through at least one third beam splitter, and each third beam splitter corresponds to one band pass filter.
For example, the first continuous wave laser and the second continuous wave laser emit laser light simultaneously.
For example, the pulse laser pulses at the same frequency as the frame rate of the photodetector.
For example, the first spatial dispersive device is a diffraction grating.
For example, the numerical apertures of the first objective lens and the second objective lens are the same.
For example, the magnification parameters of the first objective lens and the second objective lens are the same.
For example, the parameters of the first and second spatial dispersion devices are the same.
For example, light generated by particles in a liquid includes light generated by liquid particles in a cross-section of the microfluidic device in a direction perpendicular to the flow direction.
For example, the liquid comprises a body fluid, and the absorption parameter of the particles in the liquid is proportional to the hemoglobin content of the body fluid.
For example, a continuous wave laser is incident on a first focal point on the microfluidic device at the same location as a pulsed light source is incident on a second focal point in the microfluidic device.
For example, it may be provided that the signal conditioning means conditions the signal on the photo detection means and the signal on the particle detection means such that the two signals are synchronized in time.
According to the liquid detection method, the optical device is used for imaging, so that the detection speed is improved, and the type and the number of the particles in the liquid can be distinguished by detecting the shape of the particles in the liquid.
Those skilled in the art will appreciate that various modifications, combinations, sub-combinations, and substitutions are possible in the present disclosure depending on design requirements and other factors, provided they come within the scope of the appended claims and their equivalents.
Claims (10)
1. A liquid detection apparatus comprising: a pulse light source, a time domain dispersion device, a first space dispersion device, a first objective lens, a microflow device, a second objective lens, a second space dispersion device, a photoelectric detection device,
the pulsed light source configured to emit pulsed light;
the time domain dispersion device is configured to convert the pulse light emitted by the pulse light source from a frequency domain to a time domain to form pulse light with a broadened time domain;
the first spatial dispersion device is configured to change the propagation direction of light with different frequencies in the pulse light after the time domain broadening in the space;
the first objective lens is configured to converge the pulsed light with the propagation direction changed by the first spatial dispersion device to the microfluidic device;
the microfluidic device is configured to enable the liquid to be detected to flow at a preset speed;
the second objective lens is configured to collect the light collected by the first objective lens and passing through the microfluidic device;
the second spatial dispersion device is used for spatially recombining the light collected by the second objective lens;
the photoelectric detection device is configured to image the light recombined by the second spatial dispersion device so as to detect the particles in the liquid.
2. The apparatus of claim 1, wherein the pulsed light source comprises an infrared pulsed light source.
3. The apparatus of claim 1, further comprising an amplifier disposed between the time-domain dispersing device and the first spatial dispersing device for amplifying the time-domain broadened pulsed light and transmitting the amplified light to the first spatial dispersing device.
4. The apparatus of claim 1, further comprising, a first beam splitter,
the first spectroscope is positioned between the second objective and the second spatial dispersion device;
the light collected by the second objective lens and passing through the microfluidic device is transmitted through the first beam splitter and enters the second spatial dispersion device.
5. The apparatus of claim 1, further comprising a first continuous wave laser configured to emit continuous visible light and to inject the continuous visible light to the second objective lens;
the continuous visible light passing through the second objective lens is converged to at least one part of the microfluidic device to irradiate the liquid in the part of the microfluidic device, so that the particles in the liquid are excited to generate light.
6. The apparatus of claim 5, further comprising, a first beam splitter,
the continuous visible light emitted by the first continuous wave laser is incident to the first spectroscope;
the continuous visible light passing through the first beam splitter is reflected to the second objective.
7. The apparatus of claim 5, further comprising a first particle detection device, wherein light generated by particles in the liquid is incident on the first particle detection device, the first particle detection device detecting the generated light.
8. The apparatus of claim 7, further comprising a first band pass filter, wherein light generated by particles in the liquid is incident on the first band pass filter,
the light filtered by the first band-pass filter is incident on the first particle detection device.
9. The apparatus of claim 8, further comprising, a first beam splitter,
light generated by particles in the liquid of the microfluidic device is incident to the first spectroscope through the second spectroscope;
the first beam splitter reflects light generated by particles in the liquid to the first band pass filter.
10. The apparatus of claim 9, further comprising a first beam splitter positioned between the first beam splitter and the first band pass filter,
the light reflected by the first beam splitter passes through the first beam splitter, and the light with a first parameter of a preset threshold value in the reflected light is transmitted through the first beam splitter and enters the first band-pass filter.
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