CN114397759A - Passive laser homogenizing device and flow type microscopic high-speed imaging system - Google Patents
Passive laser homogenizing device and flow type microscopic high-speed imaging system Download PDFInfo
- Publication number
- CN114397759A CN114397759A CN202210105940.1A CN202210105940A CN114397759A CN 114397759 A CN114397759 A CN 114397759A CN 202210105940 A CN202210105940 A CN 202210105940A CN 114397759 A CN114397759 A CN 114397759A
- Authority
- CN
- China
- Prior art keywords
- laser light
- collimator
- laser
- passive
- focusing lens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003384 imaging method Methods 0.000 title claims abstract description 56
- 238000000265 homogenisation Methods 0.000 claims abstract description 48
- 239000013307 optical fiber Substances 0.000 claims abstract description 24
- 239000000835 fiber Substances 0.000 claims abstract description 20
- 239000002245 particle Substances 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 10
- 238000000386 microscopy Methods 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 8
- 239000005337 ground glass Substances 0.000 claims description 4
- 238000009792 diffusion process Methods 0.000 description 24
- 230000000694 effects Effects 0.000 description 24
- 238000005286 illumination Methods 0.000 description 17
- 230000008859 change Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 230000010354 integration Effects 0.000 description 4
- 241000276498 Pollachius virens Species 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000002123 temporal effect Effects 0.000 description 3
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0927—Systems for changing the beam intensity distribution, e.g. Gaussian to top-hat
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0938—Using specific optical elements
- G02B27/0944—Diffractive optical elements, e.g. gratings, holograms
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0938—Using specific optical elements
- G02B27/0994—Fibers, light pipes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/24—Coupling light guides
- G02B6/42—Coupling light guides with opto-electronic elements
- G02B6/4201—Packages, e.g. shape, construction, internal or external details
- G02B6/4204—Packages, e.g. shape, construction, internal or external details the coupling comprising intermediate optical elements, e.g. lenses, holograms
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Microscoopes, Condenser (AREA)
Abstract
A passive laser homogenizing device and a flow type microscopic high-speed imaging system are provided. The apparatus includes a plurality of passive devices disposed along a laser propagation path, including a first collimator, a multimode optical fiber, a second collimator, and a first diffuser, wherein the first collimator is configured to couple with the multimode optical fiber to couple laser light incident on the first collimator into the multimode optical fiber; the multimode fiber is configured to transmit laser light coupled in by the first collimator to perform first homogenization on the laser light coupled in by the first collimator; the second collimator is configured to couple with the multimode fiber to receive the first homogenized laser light and to emit the first homogenized laser light to the first diffuser; and the first scattering sheet is configured to receive the laser light emitted by the second collimator, perform second homogenization on the laser light emitted by the second collimator, and emit the second homogenized laser light to illuminate the target. The device can reduce the laser coherence during high-speed imaging and can be used for a flow type microscopic high-speed imaging system.
Description
Technical Field
The disclosure relates to the field of optical imaging, and more particularly, to a passive laser homogenizing device and a flow type microscopic high-speed imaging system.
Background
In imaging technology, it is often necessary to configure a light source for a target to illuminate the target. In particular, in high speed imaging applications, there is a need for a fast-response light source that can provide sufficient energy in short pulses for imaging and that is easily modulated. Ordinary light sources (e.g., LEDs, xenon lamps, etc.) are generally not able to meet such requirements. Laser light has advantages of high energy, good directivity, etc., and thus can be considered as an illumination light source in high-speed imaging applications.
However, lasers are generally very coherent. For example, when a target is irradiated with laser light as an illumination light source, bright and dark stripes or particles (this phenomenon is also referred to as speckle) appear on the target surface, which affects the imaging effect. In order to eliminate speckle and reduce coherence of laser, in the prior art, a laser homogenization method of vibrating a component and the like is generally adopted, and high-frequency vibration of the component is used for generating phase difference and the like to homogenize the laser so as to reduce coherence of the laser.
With the advancement of technology, the demand for imaging speed is increasing. Increasing the imaging speed may increase the frequency of illumination of the flowing particles to generate more images of the particles per unit time. In this case, the exposure time of the laser light for irradiating the target in the imaging must be very short, whereas the vibration frequency of the vibration element in the related art is low, which fails to achieve the intended homogenization effect in a time much lower than the vibration cycle (i.e., in the exposure time).
Therefore, a laser homogenizing device and a corresponding imaging system that can be used for high-speed imaging are desired.
Disclosure of Invention
In order to solve the above problems, the present disclosure provides a passive laser homogenizing device and a flow type microscopic high-speed imaging system. The laser homogenizing device can reduce the coherence of laser when high-speed imaging is carried out so as to improve the illumination effect. The flow type microscopic high-speed imaging system can amplify and image particles at high speed under the condition of adopting a passive laser homogenizing device, thereby obtaining particle images with better imaging effect.
According to an aspect of the present disclosure there is provided a passive laser homogenizing device comprising a plurality of passive devices disposed along a laser propagation path, the passive devices comprising a first collimator, a multimode optical fibre, a second collimator and a first scattering sheet, wherein the first collimator is configured to couple with the multimode optical fibre to couple laser light incident on the first collimator into the multimode optical fibre; the multimode fiber is configured to transmit laser light coupled in by the first collimator to first homogenize the laser light coupled in by the first collimator; the second collimator is configured to couple with the multimode fiber to receive the first homogenized laser light and to emit the first homogenized laser light to the first diffuser; and the first scattering sheet is configured to receive the laser light emitted by the second collimator, perform second homogenization on the laser light emitted by the second collimator, and emit second homogenized laser light to illuminate the target.
According to some embodiments of the present disclosure, the passive device further comprises a focusing lens located before the first collimator on the laser propagation path, the focusing lens configured to receive incident laser light, focus the incident laser light, and emit the focused laser light to the first collimator.
According to some embodiments of the present disclosure, the passive device further comprises a second scattering sheet located before the focusing lens on the laser propagation path, the second scattering sheet being configured to receive incident laser light, subject the incident laser light to a third homogenization, and emit the laser light after the third homogenization to the focusing lens.
According to some embodiments of the present disclosure, the passive device further includes a second scattering sheet located between the focusing lens and the first collimator on the laser propagation path, the second scattering sheet is configured to receive the laser light emitted from the focusing lens, perform third homogenization on the laser light emitted from the focusing lens, and emit the laser light after the third homogenization to the first collimator.
According to some embodiments of the disclosure, the focusing lens is further configured to be at a distance from a laser source generating the laser light greater than or equal to a focal length of the focusing lens.
According to some embodiments of the present disclosure, wherein the aperture of the focusing lens is greater than or equal to a beam diameter of the laser light incident to the focusing lens.
According to some embodiments of the present disclosure, wherein a collecting aperture of the first collimator is greater than or equal to a beam diameter of the laser light incident to the first collimator, and a collecting angle of the first collimator is greater than or equal to a divergence angle of the laser light incident to the first collimator.
According to some embodiments of the present disclosure, a size of the first diffusion sheet is greater than or equal to a beam diameter of the laser light emitted from the second collimator to the first diffusion sheet.
According to some embodiments of the present disclosure, the laser light has a wavelength equal to an operating wavelength of one or more of the first collimator, the multimode optical fiber, the second collimator, the first diffuser, the focusing lens, and the second diffuser.
According to some embodiments of the present disclosure, the first and second diffusion sheets comprise ground glass, an engineered diffusion sheet, or a diffractive optical element.
According to some embodiments of the disclosure, wherein the laser comprises a pulsed laser.
According to another aspect of the present disclosure, there is also provided a flow microscopy high-speed imaging system comprising: a laser source, a passive laser homogenization apparatus, a microfluidic apparatus, and an imaging apparatus as described above, wherein the laser source is configured to generate laser light; the passive laser homogenizing device is configured to homogenize the laser light so as to illuminate the liquid to be detected in the microfluidic device with the homogenized laser light; the microfluidic device is configured to control the particles in the liquid to be detected to stably flow through the microfluidic device by controlling the flow of the liquid to be detected; the imaging device comprises a microscope objective, a cylindrical lens and an image acquisition assembly; the micro objective adopts an infinite objective, focuses on the center of the microfluidic device, and images the particles flowing through; the cylindrical lens converges and images the emergent light of the infinite objective lens, and the focal length of the cylindrical lens is adjusted to adjust the imaging magnification; and the image acquisition assembly is positioned behind the cylindrical lens and shoots the amplified image to obtain a particle image.
Therefore, according to the passive laser homogenizing device disclosed by the embodiment of the disclosure, the passive multimode optical fiber and the scattering sheet are used for respectively carrying out first homogenization and second homogenization on the laser, so that the coherence of the laser is reduced under the condition of high-speed imaging to improve the illumination effect. In addition, the flow type microscopic high-speed imaging system according to the embodiment of the disclosure can homogenize laser in the system by using a passive laser homogenizing device, and under the irradiation of the homogenized laser on flowing particles in a microfluidic device of the system, the particles are amplified and imaged at a high speed, so that a particle image with a good imaging effect is obtained.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings used in the description of the embodiments will be briefly introduced below. It is apparent that the drawings in the following description are only exemplary embodiments of the disclosure, and that other drawings may be derived from those drawings by a person of ordinary skill in the art without inventive effort.
Fig. 1 shows a block diagram of a passive laser homogenization device according to a first embodiment of the present disclosure;
fig. 2 shows a block diagram of a passive laser homogenization device according to a second embodiment of the present disclosure;
fig. 3 shows a block diagram of a passive laser homogenization device according to a third embodiment of the present disclosure;
fig. 4 shows a schematic diagram of a flow microscopy high speed imaging system according to a fourth embodiment of the present disclosure.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be described below clearly and completely with reference to the accompanying drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without any inventive step, are within the scope of protection of the disclosure.
Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item preceding the word covers the element or item listed after the word and its equivalent, but not the other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly. To maintain the following description of the embodiments of the present disclosure clear and concise, a detailed description of some known functions and components have been omitted from the present disclosure.
In the specification and drawings of the present disclosure, elements are described in singular or plural forms according to embodiments. However, the singular and plural forms are appropriately selected for the proposed cases only for convenience of explanation and are not intended to limit the present disclosure thereto. Thus, the singular may include the plural and the plural may also include the singular, unless the context clearly dictates otherwise.
< first embodiment >
Fig. 1 shows a schematic diagram of a passive laser homogenization device 100 according to a first embodiment of the present disclosure. As shown in fig. 1, passive laser homogenizing device 100 can include a plurality of passive devices disposed along the laser propagation path. The passive devices include a first collimator 110, a multimode optical fiber 120, a second collimator 130, and a first diffuser 140. The first collimator 110 is configured to couple with the multimode optical fiber 120 to couple laser light incident on the first collimator 110 into the multimode optical fiber 120. The multimode optical fiber 120 is configured to transmit the laser light coupled in by the first collimator 110 to perform a first homogenization on the laser light coupled in by the first collimator 110. The second collimator 130 is configured to couple with the multimode fiber 120 to receive the first homogenized laser light and to emit the first homogenized laser light to the first diffuser 140. The first diffusion sheet 140 is configured to receive the laser light emitted from the second collimator 130, perform a second homogenization on the laser light emitted from the second collimator 130, and emit the second homogenized laser light to illuminate the target.
Specifically, first, the first collimator 110 may be disposed at a front position along the light propagation path, which is configured to couple with the multimode optical fiber 120 to couple laser light from a laser, which is incident on the first collimator 110, or laser light after passing through other elements, into the multimode optical fiber 120, thereby achieving a good light-receiving effect.
According to the first embodiment of the present disclosure, the collecting aperture of the first collimator 110 may be greater than or equal to the beam diameter of the laser light incident to the first collimator 110, and the collecting angle of the first collimator 110 is greater than or equal to the divergence angle of the laser light incident to the first collimator 110. For example, if the beam of the laser light incident on the surface of the first collimator 110 has a diameter of 1 millimeter (mm) and a divergence angle of 10 degrees, the light-collecting aperture of the first collimator 110 may be greater than or equal to 1mm and the light-collecting angle thereof may be greater than or equal to 10 degrees.
According to one embodiment of the present disclosure, the laser is a multimode laser, rather than a single mode laser, having multiple modes. In one example, the laser may be a pulsed laser, which may have a narrower pulse width and a higher pulse frequency.
With continued reference to FIG. 1, following the first collimator 110 along the path of the laser light propagation is a multimode optical fiber 120. Both ends of the multimode fiber 120 are coupled with the first collimator 110 and the second collimator 130, respectively, which may be configured to transmit the laser light coupled in by the first collimator 110 in the fiber path of the multimode fiber 120 to perform a first homogenization on the laser light coupled in by the first collimator 110.
As described above, the laser is a multimode laser, and when the multimode laser is transmitted through the multimode fiber 120, the transmission speed, direction, and the like of different modes of the laser are different, so that multimode aliasing (which can be regarded as superposition of multiple light sources) occurs when the multimode laser is transmitted through the multimode fiber 120. This phenomenon reduces the temporal coherence of the laser, provides uniformity of the beam, and thus reduces the speckle that may occur. The multimode optical fiber may be arranged in a linear form or in a non-linear form (i.e., in a curved form).
It should be noted that in the case of high speed imaging, speckle cannot be reduced by creating an integration effect on multimode fiber vibrations. The integral effect means that the vibration of the optical fiber can cause the speckle to change rapidly, and the speckle can be regarded as the integral superposition of the speckle at different moments in time within a long enough exposure time, so that the effect of speckle reduction is presented. For high-speed imaging, the low vibration frequency causes the speckle to change not fast enough in a very short exposure time, and further causes unstable homogenization, which not only fails to form an integration effect, but also may affect the illumination effect (e.g., a flicker phenomenon). Therefore, in high speed imaging applications, it is preferable to first homogenize the laser light using a passive multimode fiber to reduce the temporal coherence of the laser light, thereby enhancing the illumination effect.
With continued reference to FIG. 1, following the multimode optical fiber 120 along the laser propagation path is a second collimator 130. The second collimator 130 may be configured to couple with the multimode fiber 120 to receive the first homogenized laser light and to emit the first homogenized laser light to the first diffuser 140.
Then, the first diffusion sheet 140 is configured to receive the laser light emitted from the second collimator 130, perform second homogenization on the laser light emitted from the second collimator 130, and emit second homogenized laser light to illuminate the target.
According to the first embodiment of the present disclosure, the first diffusion sheet 140 may include ground glass, an engineered diffusion sheet, a diffractive optical element, or the like, and thus the first diffusion sheet 140 may reduce spatial coherence of the laser light by the second homogenization, thereby homogenizing the laser light to eliminate speckle.
It should be noted that the first diffusion sheet 140 is passive, that is, the first diffusion sheet 140 is not controlled by an external source, such as rotation. The rotation generally causes the speckle that may appear in the laser light emitted from the first scattering sheet 140 to change rapidly, thereby generating an integration effect. However, for high-speed imaging, since the rotation speed (i.e., the rotation period) is far shorter than the exposure time (for example, the first scattering sheet may rotate only a few degrees or a dozen degrees during one exposure time), the homogenization is unstable, and not only the integration effect cannot be formed, but also the illumination effect (for example, the flicker phenomenon occurs) may be affected.
According to the first embodiment of the present disclosure, the size of the first diffusion sheet 140 may be greater than or equal to the beam diameter of the laser light emitted from the second collimator 130 to the first diffusion sheet 140. For example, if the beam size of the laser light emitted from the second collimator 130 to the first diffusion sheet 140 is 5mm, the size of the first diffusion sheet 140 may be greater than or equal to 5mm to ensure that all the laser light can be projected through the first diffusion sheet 140.
Further, according to the first embodiment of the present disclosure, the passive devices in the passive laser homogenizing device 100, i.e., one or more of the first collimator 110, the multimode optical fiber 120, the second collimator 130, and the first scattering sheet 140, may have a wavelength consistent with the wavelength of the laser light. Preferably, the operating wavelength of all passive devices coincides with the wavelength of the laser. For example, the wavelength of the laser light is 550 nanometers (nm), and the operating wavelength of the passive device may also be 550 nm.
In addition, in one example, passive laser homogenizing device 100 can also include components (not shown), such as mirrors, to alter the laser light propagation path, thereby facilitating the placement of the entire passive laser homogenizing device 100.
In one example, passive laser homogenization apparatus 100 may also include a light source shielding assembly (not shown) that may be configured to shield a light source from the outside world to avoid contaminating the laser light to be used for illumination.
Therefore, according to the passive laser homogenizing device 100 of the first embodiment of the present disclosure, the laser light is subjected to the first homogenization (i.e., temporal coherence elimination) and the second homogenization (i.e., spatial coherence elimination) respectively by the passive multimode fiber 120 and the diffuser 130, thereby achieving the purpose of reducing the coherence of the laser light in the case of high-speed imaging to improve the illumination effect.
< second embodiment >
The present disclosure provides a passive laser homogenizing device 200, which will be described in detail with reference to fig. 2, in addition to the above-mentioned passive laser homogenizing device 100. Some components of the passive laser homogenizing device 200 are the same as those of the passive laser homogenizing device 100 of fig. 1, which are indicated by the same reference numerals in fig. 2 and are not described again.
As shown in fig. 2, according to the second embodiment of the present disclosure, a focusing lens 210 is further included before the first collimator 110 along the laser propagation path. The focusing lens 210 may be configured to focus incident laser light from the laser or incident laser light after passing through other elements before the first collimator 110 and to emit the focused laser light to the first collimator 110. By focusing the laser light through the focusing lens 210, the light beam can be focused into the light receiving aperture of the first collimator 110, so as to achieve better light receiving effect.
According to the second embodiment of the present disclosure, the focusing lens 210 may also be configured to have a distance from the laser source generating the laser light greater than or equal to the focal length f of the focusing lens 210 to achieve a desired focusing effect. Further, according to the second embodiment of the present disclosure, the aperture of the focusing lens 210 may be greater than or equal to the beam diameter of the laser light incident to the focusing lens 210 to focus the entire laser light. For example, if the diameter of the laser light incident on the surface of the focusing lens 210 is 3mm, the aperture of the focusing lens 210 may be greater than or equal to 3 mm.
According to the second embodiment of the present disclosure, the focusing lens 210 may be a focusing lens group capable of performing a focusing function, such a focusing lens group including one or more optical lenses. For example, when the focusing lens group contains only one lens, it may be a convex lens. When the focusing lens group includes a plurality of optical lenses, it may be a combination of convex lenses and/or concave lenses. In addition, in the focusing lens group, a plane mirror may be further included, which may change a path along which the laser light travels, thereby more easily achieving the arrangement of the focusing lens group.
Further, as a passive device, the operating wavelength of the focusing lens 210 may also coincide with the wavelength of the laser light.
Therefore, according to the passive laser homogenizing device 200 of the first embodiment of the present disclosure, the focusing lens 210 enables the laser light to be better coupled into the multimode fiber 120, and the passive multimode fiber 120 and the diffuser 130 perform the first homogenization and the second homogenization on the laser light respectively, thereby achieving the purpose of reducing the coherence of the laser light in the case of high-speed imaging to improve the illumination effect.
< third embodiment >
The present disclosure provides a passive laser homogenizing device 300, which will be described in detail with reference to fig. 3, in addition to the above-mentioned passive laser homogenizing devices 100, 200. Some components of the passive laser homogenizing device 300 are the same as those of the passive laser homogenizing devices 100, 200 of fig. 1 and 2, which are shown with the same reference numerals in fig. 3 and are not described again.
As shown in fig. 3, a focusing lens 210 may be included in the passive laser homogenization apparatus 300. In addition, a second diffusion sheet 310 may be further included before the focusing lens 210 on the laser propagation path. The second diffusion sheet 310 may be configured to receive the incident laser light from the laser or the incident laser light after passing through other elements, perform third homogenization on the incident laser light, and emit the third homogenized laser light to the focusing lens 210 before the focusing lens 210.
According to the third embodiment of the present disclosure, similar to the first diffusion sheet 140, the second diffusion sheet 310 may also include ground glass, an engineered diffusion sheet, a diffractive optical element, or the like, and thus the second diffusion sheet 310 may reduce spatial coherence of laser light by third homogenization, thereby homogenizing the laser light to eliminate speckle. As a passive device, the operating wavelength of the second scattering sheet 310 may also coincide with the wavelength of the laser light.
Furthermore, there are also variations to the third embodiment of the present disclosure. For example, the second diffusion sheet 310 may also be located between the focusing lens 210 and the first collimator 110 on the laser propagation path. In this case, the second diffusion sheet 310 may be configured to receive the laser light emitted from the focusing lens 210, perform third homogenization on the laser light emitted from the focusing lens 210, and emit the third homogenized laser light to the first collimator 110.
It should be noted that in some variations of the third embodiment of the present disclosure, passive laser homogenization device 300 may also include configurations without focusing lens 210.
Therefore, according to the passive laser homogenizing device 300 of the third embodiment of the present disclosure, the laser light is subjected to the third homogenization by the passive second scattering sheet 310, and further subjected to the first homogenization and the second homogenization by the multimode fiber 120 and the scattering sheet 130, respectively, thereby achieving the purpose of reducing the coherence of the laser light in the case of high-speed imaging to improve the illumination effect.
< fourth embodiment >
The present disclosure provides, in addition to the passive laser homogenization devices 100, 200, and 300 described above, a flow microscopy high-speed imaging system 400, which will be described in detail below in conjunction with fig. 4.
As depicted in fig. 4, the flow microscopy high-speed imaging system 400 may include a laser source 410, a passive laser homogenization device 420, a microfluidic device 430, and an imaging device 440. Passive laser homogenizing device 420 can comprise any of passive laser homogenizing devices 100, 200, and 300 described above. Laser source 410 may be configured to generate laser light. The passive laser homogenization device 420 is configured to homogenize the laser light to illuminate the liquid to be detected in the microfluidic device 430 with the homogenized laser light. The microfluidic device 430 is configured to control the stable flow of particles in the liquid to be detected through the microfluidic device 430 by controlling the flow of the liquid to be detected. Imaging device 440 includes a microscope objective 442, a tube lens 444, and an image capture assembly 446. The microscope objective 442 is an infinity objective focused at the center of the microfluidic device 430 to image the particles flowing through. The tube lens 444 focuses the light emitted from the infinity objective lens to form an image, and the focal length of the tube lens 444 can be adjusted to adjust the magnification of the image. The image capture assembly 446 is positioned behind the barrel mirror 44 and captures the magnified image to obtain a particle image.
In particular, the laser source 410 may be configured to generate laser light. The laser is a multimode laser. In one example, the laser source 410 may include a laser diode, a vertical cavity surface laser transmitter (VCSEL), or any other laser. In one example, laser source 410 may be configured to emit pulsed laser light. The pulsed laser may have a higher pulse frequency and a narrower pulse width. In addition, the laser source 410 may also provide a pulsed laser with a higher energy, so that sufficient illumination may be provided to the target within a narrower pulse width illumination time. Additionally or alternatively, the laser 112 may also be aggregated from multiple lasers to emit a stronger pulsed laser.
In addition, the laser source 410 may further include other components, for example, a control component configured to determine a pulse width and a pulse frequency of the laser according to the flow velocity of the flowing particles and an image acquisition frame rate of an image acquisition component (e.g., a camera), respectively, and generate a control signal for driving the laser and a synchronization trigger signal corresponding to the control signal for the image acquisition component according to the determined pulse width and pulse frequency; and the driving component is configured to generate a driving current according to the control signal so as to drive the laser to emit the pulse laser with the determined pulse width and pulse frequency.
For some specific details of the implementation of the passive laser homogenizing device 420 in the flow microscopy high-speed imaging system 400, reference may be made to the description with respect to fig. 1-3, and thus will not be further described here.
Additionally, in the flow microscopy high-speed imaging system 400, a condenser lens group 450 may also be packaged, which may be configured to collect the beam from the passive laser homogenization device 420 and to uniformly impinge the beam on the microfluidic device 430. Condenser lens group 450 may constitute Kohler (Kohler) illumination with laser source 410, passive laser homogenizer 420. The kohler illumination can change the concentric light beam emitted by the light source into a plurality of parallel light beams, and the parallel light beams uniformly irradiate on the target object (such as the micro-flow device 430 here, and more specifically, particles in the micro-flow device 430), so that the illumination can be further uniform and the light efficiency is high, and the imaging effect of the imaging system is better. Condenser lens group 450 may include two lenses, a first lens disposed near passive laser homogenizer 420, and a second lens disposed after the first lens (i.e., further from passive laser homogenizer 420 than the first lens), the first lens condensing the beam from passive laser homogenizer 420 at a front focal plane of the second lens. The condenser lens group 450 may further include more lenses as long as the light beam from the passive laser homogenizing device 420 can be uniformly emitted.
In one example, microfluidic device 430 may be a flow conduit. A sample stream containing particles may flow within microfluidic device 430. The sheath fluid encapsulating the sample particle stream enters microfluidic device 430 as a sample stream, and the particles in the sample stream flow through the center of microfluidic device 430 by controlling the flow of the sample stream in microfluidic device 430.
It should be noted that the microfluidic may be cells in a body fluid, and the particles may be based on a tangible component in the river water during the detection of the quality of the river water or any other component that can be imaged in the liquid that passes through the microfluidic device, which is not limited by the present disclosure.
In one example, microscope objective 442 may be configured to focus on a center of microfluidic device 430, collect light that passes through particles in microfluidic device 430, where microscope objective 442 operates at a distance greater than the distance of the center of microfluidic device 430 from an outer surface of microfluidic device 430, and a Numerical Aperture (NA) of microscope objective 442 is determined based on a predetermined resolution and the wavelength of the light beam.
For example, microscope objective 442 may include one or more lenses. Alternatively, the microscope objective 442 may be an infinity objective, and light passing through the infinity objective is directed toward infinity as a parallel beam. The application of an infinity objective to the microscope 442 can reduce the effect of aberrations on the observation to a greater extent.
The telescope 444 may be positioned between the microscope lens 442 and the image plane and may be configured to, in conjunction with the microscope lens 442, magnify the image of the particle and converge the magnified image of the particle onto the image plane. That is, the microscope lens 442 and the barrel lens 444 together have a magnifying function, and the magnification may be determined based on the focal lengths of the microscope lens 442 and the barrel lens 444.
For example, the barrel mirror 444 may include one or more lenses. The tube lens 444 can converge the light emitted from the infinity objective to form an image on a finite image plane. Adjusting the focal length of the barrel mirror 444 can adjust the magnification of the imaging.
The image acquisition assembly 446 is disposed on the image plane and is configured to acquire an image of the magnified microparticle on the image plane.
In one example, the image capture component 446 may be a device having image capture functionality, such as a camera, video camera, or the like. The image capture assembly 446 may also be other devices that include a sensor (such as a CCD or the like) having image capture functionality. The image capture assembly 446 and the laser source 410 may be configured to operate in concert to achieve high-speed imaging of particles with lower exposure times and higher capture frequencies.
Additionally, in one example, the flow microscopy high-speed imaging system 400 may further include a light source shielding module (not shown) configured to shield the capture particles from light sources other than homogenized laser light from the passive laser homogenization device 420. In one example, the light source shielding module can be a light-tight housing in which, for example, the entire flow microscopy high-speed imaging system 400 or the portion to be imaged of the particles can be disposed and which has an opening to allow injection of homogenized laser light from the passive laser homogenization device 420 to illuminate the particles. In another example, the light source shielding module may also be a housing made of an optical material with a fixed operating wavelength to allow only the homogenized laser light from the passive laser homogenizing device 420 with the same wavelength to be injected to irradiate the particles.
In one example, the flow microscopy high speed imaging system 400 also includes one or more power supplies that may be coupled with the laser source 410 and the imaging device 440, thereby enabling the laser source 410 and the image acquisition assembly 446 in the imaging device 440.
Therefore, according to the flow microscope high-speed imaging system 400 of the fourth embodiment of the present disclosure, the laser from the laser source 410 can be first homogenized and second homogenized by the passive multimode fiber and the scattering sheet in the passive laser homogenizing device 420 to eliminate the speckle of the laser, and the homogenized laser irradiates the flowing particles in the microfluidic device 430 of the system to image the particles in a high-speed manner, so as to obtain the particle image with better imaging effect.
Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The foregoing is illustrative of the present disclosure and is not to be construed as limiting thereof. Although a few exemplary embodiments of this disclosure have been described, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this disclosure. Accordingly, all such modifications are intended to be included within the scope of this disclosure as defined in the claims. It is to be understood that the foregoing is illustrative of the present disclosure and is not to be construed as limited to the specific embodiments disclosed, and that modifications to the disclosed embodiments, as well as other embodiments, are intended to be included within the scope of the appended claims. The present disclosure is defined by the claims and their equivalents.
Claims (13)
1. A passive laser homogenizing device comprises a plurality of passive devices arranged along a laser propagation path, wherein the passive devices comprise a first collimator, a multimode optical fiber, a second collimator and a first scattering sheet, and the passive devices comprise a first collimator, a multimode optical fiber, a second collimator and a first scattering sheet
The first collimator is configured to couple with the multimode optical fiber to couple laser light incident on the first collimator into the multimode optical fiber;
the multimode fiber is configured to transmit laser light coupled in by the first collimator to first homogenize the laser light coupled in by the first collimator;
the second collimator is configured to couple with the multimode fiber to receive the first homogenized laser light and to emit the first homogenized laser light to the first diffuser; and
the first diffuser is configured to receive the laser light emitted by the second collimator, perform a second homogenization on the laser light emitted by the second collimator, and emit second homogenized laser light to illuminate a target.
2. The apparatus of claim 1, wherein the passive device further comprises a focusing lens located before the first collimator on the laser propagation path,
the focusing lens is configured to receive incident laser light, focus the incident laser light, and emit the focused laser light to the first collimator.
3. The apparatus of claim 2, wherein the passive component further comprises a second diffuser sheet positioned in the laser propagation path before the focusing lens,
the second scattering sheet is configured to receive incident laser light, subject the incident laser light to third homogenization, and emit the laser light after third homogenization to the focusing lens.
4. The apparatus of claim 2, wherein the passive device further comprises a second diffuser sheet located between the focusing lens and the first collimator on the laser propagation path,
the second scattering sheet is configured to receive the laser light emitted by the focusing lens, perform third homogenization on the laser light emitted by the focusing lens, and emit the laser light after the third homogenization to the first collimator.
5. The apparatus of any of claims 2-4, wherein the focusing lens is further configured to be at a distance from a laser source that generates laser light that is greater than or equal to a focal length of the focusing lens.
6. The apparatus of any of claims 2-4, wherein an aperture of the focusing lens is greater than or equal to a beam diameter of laser light incident to the focusing lens.
7. The apparatus of any of claims 2-4, wherein the focusing lens is a focusing lens group comprising one or more optical lenses.
8. The apparatus of any of claims 1-4, wherein a acceptance aperture of the first collimator is greater than or equal to a beam diameter of the laser light incident to the first collimator, and a acceptance angle of the first collimator is greater than or equal to a divergence angle of the laser light incident to the first collimator.
9. The apparatus of any one of claims 1-4, wherein the first diffuser plate has a size greater than or equal to a beam diameter of the laser light exiting the second collimator to the first diffuser plate.
10. The apparatus of claim 3 or 4, wherein the laser light has a wavelength equal to an operating wavelength of one or more of the first collimator, the multimode optical fiber, the second collimator, the first diffuser, the focusing lens, and the second diffuser.
11. The device of claim 3 or 4, wherein the first and second diffuser sheets comprise ground glass, engineered diffuser sheets, or diffractive optical elements.
12. The apparatus of any of claims 1-4, wherein the laser comprises a pulsed laser.
13. A flow microscopy high-speed imaging system comprising: a laser source, a passive laser homogenization apparatus, a microfluidic apparatus, and an imaging apparatus of any of claims 1-12, wherein
The laser source is configured to generate laser light;
the passive laser homogenizing device is configured to homogenize the laser light so as to illuminate the liquid to be detected in the microfluidic device with the homogenized laser light;
the microfluidic device is configured to control the particles in the liquid to be detected to stably flow through the microfluidic device by controlling the flow of the liquid to be detected;
the imaging device comprises a microscope objective, a cylindrical lens and an image acquisition assembly;
the micro objective adopts an infinite objective, focuses on the center of the microfluidic device, and images the particles flowing through;
the cylindrical lens converges and images the emergent light of the infinite objective lens, and the focal length of the cylindrical lens is adjusted to adjust the imaging magnification; and
the image acquisition assembly is positioned behind the barrel lens and shoots the amplified image to obtain a particle image.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210105940.1A CN114397759A (en) | 2022-01-28 | 2022-01-28 | Passive laser homogenizing device and flow type microscopic high-speed imaging system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210105940.1A CN114397759A (en) | 2022-01-28 | 2022-01-28 | Passive laser homogenizing device and flow type microscopic high-speed imaging system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114397759A true CN114397759A (en) | 2022-04-26 |
Family
ID=81232756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210105940.1A Pending CN114397759A (en) | 2022-01-28 | 2022-01-28 | Passive laser homogenizing device and flow type microscopic high-speed imaging system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114397759A (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101826700A (en) * | 2009-12-24 | 2010-09-08 | 哈尔滨工业大学 | 2 mu m single mode fiber collimator with high coupling efficiency for semiconductor laser |
| CN201622390U (en) * | 2010-01-20 | 2010-11-03 | 安徽华东光电技术研究所 | Laser projection display system |
| CN104252047A (en) * | 2013-06-26 | 2014-12-31 | Cq科技有限公司 | Laser projection system with speckle elimination function |
| CN205485084U (en) * | 2016-02-04 | 2016-08-17 | 福建中科晶创光电科技有限公司 | Laser dissipation spot system |
| CN207114870U (en) * | 2017-07-19 | 2018-03-16 | 深圳小孚医疗科技有限公司 | A kind of illuminator for micro-imaging |
| CN108732694A (en) * | 2018-08-06 | 2018-11-02 | 南京邮电大学 | A kind of fiber coupling output system |
| CN109188621A (en) * | 2018-08-29 | 2019-01-11 | 浙江光塔节能科技有限公司 | A kind of multimode fibre coupled system |
| CN109799075A (en) * | 2019-02-22 | 2019-05-24 | 苏州迅镭激光科技有限公司 | The measuring device and measuring method of thermal lens focal variation in a kind of laser Machining head |
| CN110031979A (en) * | 2019-05-21 | 2019-07-19 | 中国工程物理研究院流体物理研究所 | Light spot homogenizing expands method and device |
| CN110618131A (en) * | 2019-09-12 | 2019-12-27 | 西北工业大学深圳研究院 | Large-field super-resolution fluid microscopic imaging system and implementation method thereof |
| CN210038347U (en) * | 2019-05-21 | 2020-02-07 | 中国工程物理研究院流体物理研究所 | Light spot homogenizing and beam expanding device and imaging device |
| CN111855508A (en) * | 2020-07-22 | 2020-10-30 | 天津凌视科技有限公司 | Liquid detection device and liquid detection method |
| CN113624666A (en) * | 2021-09-07 | 2021-11-09 | 清华大学 | Stream type imaging system based on dot matrix laser scanning |
| CN113835232A (en) * | 2021-09-30 | 2021-12-24 | 华中科技大学 | Speckle dissipation device, laser projection imaging system and laser illumination imaging system |
-
2022
- 2022-01-28 CN CN202210105940.1A patent/CN114397759A/en active Pending
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101826700A (en) * | 2009-12-24 | 2010-09-08 | 哈尔滨工业大学 | 2 mu m single mode fiber collimator with high coupling efficiency for semiconductor laser |
| CN201622390U (en) * | 2010-01-20 | 2010-11-03 | 安徽华东光电技术研究所 | Laser projection display system |
| CN104252047A (en) * | 2013-06-26 | 2014-12-31 | Cq科技有限公司 | Laser projection system with speckle elimination function |
| CN205485084U (en) * | 2016-02-04 | 2016-08-17 | 福建中科晶创光电科技有限公司 | Laser dissipation spot system |
| CN207114870U (en) * | 2017-07-19 | 2018-03-16 | 深圳小孚医疗科技有限公司 | A kind of illuminator for micro-imaging |
| CN108732694A (en) * | 2018-08-06 | 2018-11-02 | 南京邮电大学 | A kind of fiber coupling output system |
| CN109188621A (en) * | 2018-08-29 | 2019-01-11 | 浙江光塔节能科技有限公司 | A kind of multimode fibre coupled system |
| CN109799075A (en) * | 2019-02-22 | 2019-05-24 | 苏州迅镭激光科技有限公司 | The measuring device and measuring method of thermal lens focal variation in a kind of laser Machining head |
| CN110031979A (en) * | 2019-05-21 | 2019-07-19 | 中国工程物理研究院流体物理研究所 | Light spot homogenizing expands method and device |
| CN210038347U (en) * | 2019-05-21 | 2020-02-07 | 中国工程物理研究院流体物理研究所 | Light spot homogenizing and beam expanding device and imaging device |
| CN110618131A (en) * | 2019-09-12 | 2019-12-27 | 西北工业大学深圳研究院 | Large-field super-resolution fluid microscopic imaging system and implementation method thereof |
| CN111855508A (en) * | 2020-07-22 | 2020-10-30 | 天津凌视科技有限公司 | Liquid detection device and liquid detection method |
| CN113624666A (en) * | 2021-09-07 | 2021-11-09 | 清华大学 | Stream type imaging system based on dot matrix laser scanning |
| CN113835232A (en) * | 2021-09-30 | 2021-12-24 | 华中科技大学 | Speckle dissipation device, laser projection imaging system and laser illumination imaging system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5525136B2 (en) | Optical device for generating sheet light | |
| US8759804B2 (en) | Chamber apparatus and extreme ultraviolet light generation system | |
| EP3032312B1 (en) | Confocal scanner and confocal microscope | |
| JP6792600B2 (en) | EUV collector | |
| US9965867B2 (en) | Particle control device | |
| TW201215245A (en) | Alignment of light source focus | |
| JP2014531060A (en) | Equipment used for sample illumination in SPIM microscope | |
| JPH08327942A (en) | Optical apparatus for generation of sharp irradiated radiation from laser beam | |
| JP2006032322A (en) | Device for temporally stable formation of euv-ray using plasma induced by laser | |
| JP2006292782A (en) | External laser introducing device | |
| JPH11326212A (en) | Wide-visual-field fluorescent image forming device | |
| JP2017090851A (en) | Sheet illumination microscope and sheet illumination method | |
| US10018822B2 (en) | Laser microscope apparatus including photodetector having a plurality of detection elements and an adjusting mechanism for adjusting the beam diameter | |
| EP2498116B1 (en) | Microscope apparatus | |
| CN216434623U (en) | Optical lens equipment and adjustable uniform light source system | |
| US8294984B2 (en) | Microscope | |
| JP6646676B2 (en) | Extreme ultraviolet light generator | |
| JP2006275685A (en) | Dlp type evanescence microscope | |
| CN114397759A (en) | Passive laser homogenizing device and flow type microscopic high-speed imaging system | |
| US7466796B2 (en) | Condenser zone plate illumination for point X-ray sources | |
| US9069167B2 (en) | Illumination apparatus for microscope and microscope using the same | |
| JP2020137429A (en) | Cell observation system and cell observation method | |
| JP2001228402A (en) | Confocal optical scanner and confocal microscope | |
| CN111781718B (en) | Far field correction long working distance microscope structure used under overweight environment | |
| JP2012181341A (en) | Microscope device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |