CN205879793U - Capillary electrophoresis detection system - Google Patents
Capillary electrophoresis detection system Download PDFInfo
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- CN205879793U CN205879793U CN201620670439.XU CN201620670439U CN205879793U CN 205879793 U CN205879793 U CN 205879793U CN 201620670439 U CN201620670439 U CN 201620670439U CN 205879793 U CN205879793 U CN 205879793U
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Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The embodiment of the utility model provides a capillary electrophoresis detecting system, every laser instrument shines every capillary in the capillary group in proper order in the laser group, including the target in the detection sample in the every capillary and waiting to examine the material, the target is waited to examine the material and is included the biological material of the biological material that has marked fluorescent dye or is combined with the biological material who has marked fluorescent dye's antibody, every laser instrument shines the fluorescence of a plurality of wavelength ranges that fluorescent dye arouses in the every capillary in proper order, the detector that is equipped with for this laser instrument is received in proper order, and then detect the kind or the content of the material that the detection sample in the every capillary contains in proper order. The multiple capillaries are irradiated by the multiple lasers in sequence, so that detection samples of different types or different sources can be detected at one time, and multiple different target substances to be detected in the detection samples of the same type and source can also be detected at one time, the detection speed is improved, and the consumption of the detection samples is reduced.
Description
Technical field
This utility model relates to detection technique field, particularly relates to a kind of analysis system of capillary electrophoresis.
Background technology
Capillary electrophoresis (capillary electrophoresis, CE) technology is with capillary tube as split tunnel, with height
Pressure DC electric field is the Novel liquid-phase isolation technics of driving force.Capillary electrophoresis typically uses quartz capillary column, at capillary tube
Under external high pressure electric field action, the buffer of side moves to negative pole direction due to positively charged.Simultaneously, at buffer solution
In, charged biomolecules is under the outside high voltage electric field effect of capillary tube, with respective friction speed, to this charged biomolecules institute
Electrically charged opposite polarity electrode direction moves, and forms electrophoresis.
In prior art, capillary electrophoresis technique is combined with Fluorometric assay of fluorescence-labeled technology, to contained in detection sample
Biological substance detect.Labelling fluorescent dye on biological substance, during biological substance is mobile in capillary tube, glimmering
Photoinitiator dye is produced fluorescence by laser excitation, and detector receives produced fluorescence, analyzes the fluorescence received, and then detection hair
The kind of target material to be checked or content in sample in tubule.The most conventional detection means, is to use a laser instrument to excite
One capillary detects fluorescence produced by the fluorescent dye on sample detect, a kind of target can only be detected to be checked every time
Material, causes detecting speed slow, and detection sample expends big.
Utility model content
This utility model solves the technical problem that and is to provide a kind of analysis system of capillary electrophoresis and detection method, thus
Can disposably use multiple capillary detection multiple detection sample, and can disposably detect in same detection sample a large amount of
The biological substance of kind, improves detection speed, reduces the consumption of detection sample.
To this end, the technical scheme that this utility model solves technical problem is:
A kind of analysis system of capillary electrophoresis, described system includes:
Laser array, described laser array includes at least two laser instrument, and the wavelength of each laser instrument output is different；Capillary
Pipe group, described capillary tube group includes at least two capillaries, and the detection sample in every capillary includes target material to be checked,
Described target material to be checked includes that the biological substance that marked fluorescent dye or be combined with marked the antibody of fluorescent dye
Biological substance；Detector set, the number of the detector in described detector set is identical, often with the number of laser instrument in laser array
Individual laser instrument is equipped with a detector；
Every capillary during each laser instrument irradiates described capillary tube group successively in described laser array, every capillary
In detection sample in fluorescent dye excited the fluorescence producing multiple wave-length coverages by laser, each laser instrument successively according to
Penetrate the fluorescence of multiple wave-length coverages that every capillary is excited, received successively by the detector being equipped with to this laser instrument, and then
Detect the target substance classes to be checked included by sample of the detection in every capillary or content successively.
Optionally, described system also includes:
Controller, and the probe being connected with described controller, multiple spectroscopes；
Each laser instrument corresponds to a spectroscope, and each laser instrument, equipped with a detector, spectroscopical number, swashs
The number of light device, and the number of detector is the most identical；
Described probe receives the control signal of described controller, according to the scanned described capillary tube of described control signal
Group, the laser of each laser instrument output in described laser array, is swashed transmitted through this by the first optical fiber in described probe
The spectroscope that light device is corresponding, is radiated on the every capillary in described capillary tube group successively, detects sample in every capillary
In fluorescent dye be excited produced fluorescence, via the in dichroic mirror corresponding to this laser instrument to described probe
Two optical fiber, transmission is to the detector being equipped with to this laser instrument；
Every capillary in described capillary tube group is irradiated with a laser the fluorescence exciting multi-wavelength's scope, the plurality of wavelength
The laser of scope is received by detector, and this detector is the detector provisioned in the laser instrument to this capillary tube of irradiation.
Optionally, each detector includes:
Reflecting mirror, multiple optical filters, and multiple photodetector, the number of described optical filter is individual with photodetector
Number is identical, and each photodetector is arranged on after an optical filter；
Described reflecting mirror will be incident on the fluorescent reflection extremely the plurality of optical filter that described detector is received；
Each optical filter is through the fluorescence of a kind of wave-length coverage, by the fluorescent reflection of other wave-length coverages to described reflection
Mirror；
Each photodetector receives the fluorescence that the optical filter of this photodetector front setting passes through.
Optionally, described detector also includes:
Multiple focus lamps, the number of focus lamp is identical with the number of photodetector；
Before each focus lamp is arranged on a photodetector, the fluorescent foci passed through by an optical filter is to a photoelectricity
Detector.
Optionally,
Described target material to be checked includes the protein molecule in body fluid, peptide molecule, and amino acid molecular is amino acid derived
The molecule of thing, alkaloid molecule, nitrogenous class non-protein biomolecule, steroid molecule, sterols molecule, nucleic acid molecule, core
Thuja acid derivative molecular, lipid, derivative of fatty acid, saccharide, vitamin, lipoprotein, apolipoprotein, glycoprotein, mucin, gold
Belong to albumen, any one or more in glycolipid.
Optionally,
Described target material to be checked includes the protein molecule in DNA, and body fluid, peptide molecule, amino acid molecular, ammonia
The molecule of base acid derivative, alkaloid molecule, nitrogenous class non-protein biomolecule, steroid molecule, sterols molecule, nucleotide
Molecule, nucleotide derivative molecule, lipid, derivative of fatty acid, saccharide, vitamin, lipoprotein, apolipoprotein, glycoprotein, viscous
Albumen, metalloprotein, any one or more in glycolipid.
By technique scheme, this utility model has the advantages that:
This utility model embodiment provides analysis system of capillary electrophoresis and detecting system, laser array, described laser
Device group includes at least two laser instrument, and the wavelength of each laser instrument output is different；Capillary tube group, described capillary tube group includes at least
Two capillaries, the detection sample in every capillary includes that target material to be checked, described target material to be checked include labelling
The biological substance of fluorescent dye or be combined with the biological substance of the antibody that marked fluorescent dye；Detector set, described spy
The number of the detector in survey device group is identical with the number of laser instrument in laser array, and each laser instrument is equipped with a detection
Device；Every capillary during each laser instrument irradiates described capillary tube group successively in described laser array, in every capillary
Fluorescent dye in detection sample is excited the fluorescence producing multiple wave-length coverages by laser, and each laser instrument irradiates often successively
The fluorescence of multiple wave-length coverages that capillary is excited, is received successively by the detector being equipped with to this laser instrument, and then successively
Detect kind or the content of the material to be checked of the target in every capillary.Multiple laser instrument is used to irradiate many capillaries successively,
The kind detecting sample or source in every capillary can also be different, can detect different or kind difference of originating simultaneously
Detection sample；Kind and source in the detection sample of every capillary can also be identical, it is possible to disposable detection kind with
Multiple different target material to be checked in the detection sample originating identical, improves detection speed, reduces the consumption of detection sample.
Accompanying drawing explanation
In order to be illustrated more clearly that this utility model embodiment or technical scheme of the prior art, below will be to embodiment
Or the required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, the accompanying drawing in describing below is only
It is embodiments more of the present utility model, for those of ordinary skill in the art, in the premise not paying creative work
Under, it is also possible to other accompanying drawing is obtained according to these accompanying drawings.
Fig. 1 is the analysis system of capillary electrophoresis structural representation provided for this utility model embodiment；
A kind of possible structural representation of analysis system of capillary electrophoresis that Fig. 2 provides for this utility model embodiment；
A kind of panel detector structure schematic diagram that Fig. 3 provides for this utility model embodiment；
The another kind of panel detector structure schematic diagram that Fig. 4 provides for this utility model embodiment；
Fig. 5 carries out the example flow chart detected for the analysis system of capillary electrophoresis that this utility model embodiment provides.
Detailed description of the invention
In order to provide raising detection speed, reducing the implementation of the consumption of detection sample, this utility model embodiment carries
Supply a kind of analysis system of capillary electrophoresis, below in conjunction with Figure of description, preferred embodiment of the present utility model has been said
Bright.
The analysis system of capillary electrophoresis structural representation that Fig. 1 provides for this utility model embodiment, described system includes:
Laser array 101, described laser array 101 includes at least two laser instrument, and the wavelength of each laser instrument output is not
With；Capillary tube group 102, described capillary tube group 102 includes at least two capillaries, and the detection sample in every capillary includes
Target material to be checked, described target material to be checked includes the biological substance that marked fluorescent dye or is combined with and marked fluorescence
The biological substance of the antibody of dyestuff；In detector set 103, the number of the detector in described detector set 103 and laser array
The number of laser instrument is identical, and each laser instrument is equipped with a detector.
Every capillary during each laser instrument irradiates described capillary tube group 102 successively in described laser array 101, every
The fluorescent dye in detection sample in capillary tube is excited the fluorescence producing multiple wave-length coverages, each laser instrument by laser
Irradiate the fluorescence of multiple wave-length coverages that every capillary is excited successively, connect successively by the detector being equipped with to this laser instrument
Receive, detect the material contained by sample of the detection in every capillary successively.Fluorescent dye can be that to be marked directly on target to be checked
Fluorescent dye on material, it is possible to be the fluorescent dye being marked on the antibody that material to be checked with target is combined.
Laser array 101 includes at least two laser instrument, and the wavelength of each laser instrument is different.It is understood that
The laser instrument of a kind of wavelength can excite the fluorescence of a limited number of wave-length coverage, and it is glimmering that the laser instrument of different wave length can excite
The number of the wave-length coverage of light increases.Laser instrument is the most, it is possible to the kind of the wavelength of the laser of output is the most, it is possible to excite is glimmering
The number of the wave-length coverage of light is the most.The fluorescence of one wave-length coverage represents a kind of target material to be checked, then can detect
The kind of target material to be checked the most.In laser array 101, the number of laser instrument can the most specifically be arranged.
Comprising at least two capillaries in capillary tube group 102, the detection sample in every capillary is different, has how many hairs
Tubule just can detect the detection sample in how many kinds or source simultaneously.Capillary inner diameter employed in capillary tube group 102 is 5 micro-
Rice is to 100 microns, and external diameter is 50 microns to 300 microns, a length of 3 centimetres to 80 centimetres.Capillary tube in capillary tube group 102
Number can the most specifically be arranged.Such as, in Fig. 1, in capillary tube group 102, the quantity of capillary tube is 32, in reality
In application, it is not limited in the quantity of the capillary tube shown in Fig. 1.Such as, capillary tube group can include 2 capillaries, can wrap
Include 10 capillaries, it is also possible to include 384 capillaries etc..
Each laser instrument in laser array 101 irradiates the every capillary in capillary tube group 102 successively.Illustrate:
A laser instrument a1 in laser array, irradiates the every capillary in capillary tube group 102 successively, and laser instrument a1 irradiates capillary tube
When organizing the capillary in 102, the fluorescent dye in detection sample in this capillary tube can be inspired fluorescence；Laser instrument a1
When irradiating another capillary in capillary tube group 102, the fluorescent dye in detection sample in this capillary tube also can be excited
Go out fluorescence.I.e. laser instrument a1 irradiates which capillary in capillary tube group 102, detects the fluorescent dye in sample all in this capillary tube
Fluorescence can be inspired.Assume to comprise in capillary tube group 102 384 capillaries, then 384 capillaries are in the irradiation of laser instrument a1
Under all can be inspired fluorescence.Premise is, marked and can be swashed in capillary tube group 102 on the detection sample in every capillary
Light device a1 inspires the fluorescent dye of fluorescence.By that analogy, each laser instrument such as swashing in examples detailed above in laser array 101
Light device a1, irradiates the every capillary in capillary tube group 102 successively.
Detector set 103 includes multiple detector, and the number of detector is identical with the number of laser instrument.Each laser instrument
It is equipped with a detector.The fluorescence that each laser instrument in laser array 101 excites, by the detection being equipped with to this laser instrument
Device receives.Illustrate: if detector b1 is the detector being equipped with to laser instrument a1, then laser instrument a1 irradiates described capillary tube group
In 102, fluorescence produced by every capillary, is all received by detector b1.
In one example, as in figure 2 it is shown, described system also includes:
Controller 201, and the probe 202 being connected with described controller 201, multiple spectroscopes.
Each spectroscope corresponds to a laser instrument, and each laser instrument, equipped with a detector, spectroscopical number, swashs
The number of light device is the most identical with the number of detector.
Described probe 202 receives the control signal of described controller 201, according to the scanned described hair of described control signal
Tubule group 102, the laser of each laser instrument output in laser array 101 is saturating by the first optical fiber in described probe 202
It is emitted through the spectroscope that this laser instrument is corresponding, is radiated at successively on the every capillary in described capillary tube group 102, every capillary
The fluorescence that fluorescent dye in middle detection sample is inspired, via the dichroic mirror extremely described probe that this laser instrument is corresponding
The second optical fiber in 202, transmission is to the detector being equipped with to this laser instrument.
Every capillary in described capillary tube group 102 is irradiated with a laser the fluorescence exciting multiple wave-length coverage, the plurality of
The laser of wave-length coverage is received by detector, and this detector is the detector provisioned in the laser instrument to this capillary tube of irradiation.
Probe 202 includes multiple spectroscope, multiple first optical fiber, and multiple second optical fiber.Each laser instrument pair
Ying Yuyi spectroscope, first optical fiber, second optical fiber.The laser of each laser instrument output is corresponding by this laser instrument
First fiber-optic transfer, is radiated on every capillary of capillary tube group 102 through the spectroscope that this laser instrument is corresponding, capillary tube group
Every capillary of 102 detects the fluorescence that the fluorescent dye in sample is inspired, via the spectroscope that this laser instrument is corresponding
Reflex to the second optical fiber that this laser instrument is corresponding, the detection that the fluorescence received transmission is extremely equipped with by the second optical fiber to this laser instrument
Device.
Needing exist for explanation, " first " in the first optical fiber, " second " in the second optical fiber, expression is not suitable
Sequence, but a class, the first optical fiber is not an optical fiber, but a type optical fiber, the second optical fiber is not an optical fiber, but a class
Optical fiber, has multiple first optical fiber, multiple second optical fiber, the number of the first optical fiber in probe 302, the number of the second optical fiber is with sharp
The number of light device is identical, corresponding first optical fiber of each laser instrument and the second optical fiber.
Illustrate, as in figure 2 it is shown, the laser of laser instrument a1 output is transmitted by the first optical fiber x1 in probe, transmitted through
Spectroscope y1 is radiated on the every capillary in capillary tube group 102, detects in sample in every capillary of capillary tube group 102
The fluorescence that is inspired of fluorescent dye reflexed to the second optical fiber z1 in probe by spectroscope y1, by the second optical fiber z1 by fluorescence
The detector b1 that transmission is extremely equipped with to laser instrument a1；The laser of laser instrument a2 output is by the first optical fiber x2 transmission in probe, thoroughly
It is emitted through spectroscope y2 to be radiated on the every capillary in capillary tube group 102, the detection in every capillary of capillary tube group 102
The fluorescence that fluorescent dye in sample is inspired is reflexed to the second optical fiber z2 in probe by spectroscope y2, by the second optical fiber z2
By fluorescence transmission to the detector b2 being equipped with to laser instrument a2；The laser of laser instrument a3 output is passed by the first optical fiber x3 in probe
Defeated, it is radiated on the every capillary in capillary tube group 102 transmitted through spectroscope y3, the every capillary in capillary tube group 102
In detection sample in the fluorescence that is inspired of fluorescent dye reflexed to the second optical fiber z3 in probe by spectroscope y3, by the
Two optical fiber z3 are by fluorescence transmission to the detector b3 being equipped with to laser instrument a3；The laser of laser instrument a4 output is by probe first
Optical fiber x4 transmits, and is radiated on the every capillary in 102 groups of capillary tube transmitted through spectroscope y4, every in capillary tube group 102
The fluorescence that the fluorescent dye in detection sample in root capillary tube is inspired is reflexed to the second light in probe by spectroscope y4
Fine z4, by the second optical fiber z4 by fluorescence transmission to the detector b4 being equipped with to laser instrument a4.
In laser array 101, each laser instrument is swept successively and is irradiated every capillary in capillary tube group 102.Illustrate:
The a certain moment, when the laser of laser instrument a1 output irradiates capillary tube group c capillary, purpose fluorescence dye in c capillary
The fluorescence that material is inspired is received by detector b1；The laser of laser instrument a2 output irradiates capillary tube group d capillary, the
The fluorescence that fluorescent dye in d capillary is inspired is received by detector b2；The laser of laser instrument a3 output irradiates hair
During tubule group e capillary, the fluorescence that the fluorescent dye in e capillary is inspired is received by detector b3；Swash
The laser of light device a4 output irradiates capillary tube group f capillary, and it is glimmering that the fluorescent dye in f capillary is inspired
Light is received by detector b4.It follows that synchronization, the capillary tube that each laser instrument is irradiated is different.Further, it is arranged in
The laser of distance capillary tube group 102 laser instrument output farther out is not the most irradiated to any one capillary tube.
In actual applications, the number of laser instrument and detector can the most specifically be arranged, and is not limited in
Shown in Fig. 2 four laser instrument and four detectors, laser instrument is identical with the number of detector.
In actual applications, the laser of laser array 101 output can be with direct irradiation capillary in capillary tube group 102
Guan Shang, the laser of laser array 101 output can also be radiated on the capillary tube in capillary tube group 102 by after fiber-optic transfer,
Technical staff is the most specifically arranged.
Controller 201 controls the scanned described capillary tube group 102 of probe 202, so that the output of each laser instrument is sharp
Light is radiated on the every capillary in capillary tube group 102 successively via the first optical fiber in probe, and detector receives capillary
Fluorescence produced by fluorescent dye in detection sample in every capillary in pipe group 102.The direction of probe 202 scanning is such as
Shown in Fig. 2.Needing exist for explanation, the direction of probe 202 scanning can also be contrary with the scanning direction shown in Fig. 2, skill
Art personnel sets itself according to actual needs.
In one example, as it is shown on figure 3, each detector includes:
Reflecting mirror, multiple optical filters, and multiple photodetector, the number of described optical filter is individual with photodetector
Number is identical, and each photodetector is arranged on after an optical filter；
Described reflecting mirror will be incident on the fluorescent reflection extremely the plurality of optical filter that described detector is received；
Each optical filter is through the fluorescence of a wave-length coverage, by the fluorescent reflection of other wave-length coverages to described reflection
Mirror；
Each photodetector receives the fluorescence that the optical filter of this photodetector front setting passes through.
In analysis system of capillary electrophoresis, multiple detector, each detector is had to include reflecting mirror, multiple optical filterings
Sheet, and multiple photodetector.One laser instrument can excite the fluorescence of several wave-length coverage, the detection being equipped with to this laser instrument
Device receives the fluorescence of the several wave-length coverages excited.Detector includes reflecting mirror, and it is glimmering that detector is received by reflecting mirror
Luminous reflectance is to optical filter.The fluorescent reflection of other wave-length coverages, through the fluorescence of a kind of wave-length coverage, is returned instead by each optical filter
Penetrate mirror.Having a photodetector each optical filter is disposed behind, this photodetector only receives that optical filter is passed through
Plant the fluorescence of wave-length coverage.And then realize the fluorescence of all wavelengths scope that detector one laser instrument of reception is excited.Further,
In order to reduce light loss of energy, each optical filter and reflecting mirror are close to place.
As a example by Fig. 3, described detector includes a reflecting mirror 301, four optical filter p1～p4, four photodetectors
Q1～q4.The number of optical filter is identical with the number of photodetector.
Reflecting mirror 301 is relatively big, can cover all of optical filter p1～p4, each optical filter p1～p4 and this reflecting mirror
301 are close to place.The fluorescence received is reflexed to each optical filter p1～p4 by reflecting mirror 301.The fluorescence that optical filter p1 passes through
Being received by photodetector q1, the fluorescence that optical filter p2 passes through is received by photodetector q2, and it is glimmering that optical filter p3 passes through
Light is received by photodetector q3, and the fluorescence that optical filter p4 passes through is received by photodetector q4.
In actual applications, the optical filter arranged in each detector and the number of photodetector, will not be less than one
The number of the wave-length coverage of the fluorescence that laser instrument can excite.Generally, a laser instrument can excite four or five wavelength
The fluorescence of scope, arranges four or five optical filters and four or five photodetectors in a detector.
Photodetector includes charge coupled cell (Charge-coupled Device, CCD) imageing sensor, snowslide
Photodiode (Avalanche Photo Diode, APD), complementary metal oxide semiconductors (CMOS) (Complementary
Metal Oxide Semiconductor, CMOS) imageing sensor, and photomultiplier tube (Photo Multiplier
Tube, PMT) in any one.
In one example, as shown in Figure 4, described detector also includes:
Multiple focus lamps, the number of focus lamp is identical with the number of photodetector；
Before each focus lamp is arranged on a photodetector, the fluorescent foci passed through by an optical filter is to a photoelectricity
Detector.
A focus lamp, the fluorescent foci that an optical filter is passed through by this focus lamp is added before each photodetector
To photodetector.As shown in Figure 4, the fluorescent foci that optical filter p1 is passed through by focus lamp L1 is to photodetector q1；Focus lamp
The fluorescent foci that optical filter p2 is passed through by L2 is to photodetector q2；The fluorescent foci that optical filter p3 is passed through by focus lamp L3 is to light
Electric explorer q3；The fluorescent foci that optical filter p4 is passed through by focus lamp L4 is to photodetector q4.
In one example, described target material to be checked includes the protein molecule in body fluid, peptide molecule, Amino acid score
Son, the molecule of amino acid derivativges, alkaloid molecule, nitrogenous class non-protein biomolecule, steroid molecule, sterols molecule,
Nucleic acid molecule, nucleotide derivative molecule, lipid, derivative of fatty acid, saccharide, vitamin, lipoprotein, apolipoprotein, sugar
Albumen, mucin, metalloprotein, any one or more in glycolipid.
In another example, described target material to be checked includes the protein molecule in DNA, and body fluid, and polypeptide divides
Son, amino acid molecular, the molecule of amino acid derivativges, alkaloid molecule, nitrogenous class non-protein biomolecule, steroid molecule,
Sterols molecule, nucleic acid molecule, nucleotide derivative molecule, lipid, derivative of fatty acid, saccharide, vitamin, lipoprotein,
Apolipoprotein, glycoprotein, mucin, metalloprotein, any one or more in glycolipid.
The analysis system of capillary electrophoresis that this utility model provides, achieves in same detection sample, simultaneously for the first time
Detection DNA and non-DNA biological substance, detect gene test and clinical biochemical, and immunology detection is unified mutually, in clinical diagnosis
On have important breakthrough.
As shown in the above, this utility model has the advantages that:
Using multiple laser instrument to irradiate many capillaries successively, the kind of the detection sample in every capillary or source are also
Can be different, different or diverse detection sample of originating can be detected simultaneously；In the detection sample of every capillary
Kind can also be identical with source, it is possible to the multiple different target in the detection sample that disposable detection kind is identical with source
Material to be checked, improves detection speed, reduces the consumption of detection sample.
Fig. 5 carries out the example flow chart detected, institute for the analysis system of capillary electrophoresis that this utility model embodiment provides
The method of stating includes:
501: in laser array, the laser of at least two laser instrument output is radiated at least two in capillary tube group successively
On capillary tube, the wavelength of each laser output is different, and the detection sample in every capillary is different, the detection in every capillary
Sample includes plurality of target material to be checked.
502: the fluorescent dye in detection sample in the every capillary in described capillary tube group is excited by laser
Producing the fluorescence of multiple wave-length coverage, the detection sample in every capillary includes target material to be checked, and described target is to be checked
Material includes the biological substance that marked fluorescent dye or is combined with the biological substance of the antibody that marked fluorescent dye.
503: the fluorescence of the multiple wave-length coverages excited in every capillary is received by detector, this detector be to
Irradiate the detector provisioned in the laser instrument of this capillary tube.
504: detector set is according to the fluorescence of the multiple wave-length coverages received, and then detects in described capillary tube group
The kind of the target material to be checked that the detection sample in every capillary includes or content.
In one example, multiple detectors receive the fluorescence of multiple wave-length coverages, and then detect in described capillary tube
Protein molecule in detection sample, peptide molecule, amino acid molecular, the molecule of amino acid derivativges, alkaloid molecule, nitrogenous
Class non-protein biomolecule, steroid molecule, sterols molecule, nucleic acid molecule, nucleotide derivative molecule, lipid, fat
Acid derivative, saccharide, vitamin, lipoprotein, apolipoprotein, glycoprotein, mucin, metalloprotein, any one in glycolipid
Or it is multiple.
In one example, multiple detectors receive the fluorescence of multi-wavelength's scope, detect in described capillary tube simultaneously
Detection sample marked the DNA of fluorescent dye, and protein molecule, peptide molecule, amino acid molecular, amino acid derivativges
Molecule, alkaloid molecule, nitrogenous class non-protein biomolecule, steroid molecule, sterols molecule, nucleic acid molecule, nucleoside
Derivative molecules, lipid, derivative of fatty acid, saccharide, vitamin, lipoprotein, apolipoprotein, glycoprotein, mucin, metal
Albumen, any one or more in glycolipid.
Detector receives the fluorescence of multiple wave-length coverages, it is possible to the wave-length coverage of received fluorescence detected.Again with in advance
The examination criteria comparison first set, i.e. would know that in detection sample and comprises containing of which type of biological substance and this material
Amount.
Capillary electrophoresis detection example shown in Fig. 5 be with the analysis system of capillary electrophoresis shown in Fig. 1 to Fig. 4 corresponding to
Example, specific implementation, referring to figs. 1 to the description in the system shown in Fig. 4, repeats no more here.
The above is only preferred implementation of the present utility model, it is noted that for the common skill of the art
For art personnel, on the premise of without departing from this utility model principle, it is also possible to make some improvements and modifications, these improve and
Retouching also should be regarded as protection domain of the present utility model.
Claims (6)
1. an analysis system of capillary electrophoresis, it is characterised in that described system includes:
Laser array, described laser array includes at least two laser instrument, and the wavelength of each laser instrument output is different；Capillary tube
Group, described capillary tube group includes at least two capillaries, and the detection sample in every capillary includes target material to be checked, institute
State target material to be checked include marked the biological substance of fluorescent dye or be combined with the life of the antibody that marked fluorescent dye
Thing material；Detector set, the number of the detector in described detector set is identical with the number of laser instrument in laser array, each
Laser instrument is equipped with a detector；
Every capillary during each laser instrument irradiates described capillary tube group successively in described laser array, in every capillary
Fluorescent dye in detection sample is excited the fluorescence producing multiple wave-length coverages by laser, and each laser instrument irradiates often successively
The fluorescence of multiple wave-length coverages that capillary is excited, is received successively by the detector being equipped with to this laser instrument, and then successively
Detect the target substance classes to be checked included by sample of the detection in every capillary or content.
System the most according to claim 1, it is characterised in that described system also includes:
Controller, and the probe being connected with described controller, multiple spectroscopes；
Each laser instrument corresponds to a spectroscope, and each laser instrument is equipped with a detector, spectroscopical number, laser instrument
Number, and the number of detector is the most identical；
Described probe receives the control signal of described controller, according to the scanned described capillary tube group of described control signal, institute
State the laser that each laser instrument in laser array exports, by the first optical fiber in described probe transmitted through this laser instrument pair
The spectroscope answered, is radiated on the every capillary in described capillary tube group successively, and that detects in sample in every capillary is glimmering
Photoinitiator dye is excited produced fluorescence, via the second light in dichroic mirror corresponding to this laser instrument to described probe
Fibre, transmission is to the detector being equipped with to this laser instrument；
Every capillary in described capillary tube group is irradiated with a laser the fluorescence exciting multi-wavelength's scope, the plurality of wave-length coverage
Laser received by detector, this detector be to irradiate this capillary tube laser instrument provisioned in detector.
System the most according to claim 2, it is characterised in that each detector includes:
Reflecting mirror, multiple optical filters, and multiple photodetector, the number phase of the number of described optical filter and photodetector
With, each photodetector is arranged on after an optical filter；
Described reflecting mirror will be incident on the fluorescent reflection extremely the plurality of optical filter that described detector is received；
Each optical filter is through the fluorescence of a kind of wave-length coverage, by the fluorescent reflection of other wave-length coverages to described reflecting mirror；
Each photodetector receives the fluorescence that the optical filter of this photodetector front setting passes through.
System the most according to claim 3, it is characterised in that described detector also includes:
Multiple focus lamps, the number of focus lamp is identical with the number of photodetector；
Before each focus lamp is arranged on a photodetector, the fluorescent foci passed through by an optical filter is to a photodetection
Device.
5. according to the system described in claim 1-4 any one, it is characterised in that
Described target material to be checked includes the protein molecule in body fluid, peptide molecule, amino acid molecular, amino acid derivativges
Molecule, alkaloid molecule, nitrogenous class non-protein biomolecule, steroid molecule, sterols molecule, nucleic acid molecule, nucleotide
Derivative molecular, lipid, derivative of fatty acid, saccharide, vitamin, lipoprotein, apolipoprotein, glycoprotein, mucin, metal egg
In vain, any one or more in glycolipid.
System the most according to claim 5, it is characterised in that
Described target material to be checked includes DNA, and the biological substance described at least one claim 5.
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| CN105973859A (en) * | 2016-06-29 | 2016-09-28 | 山东科立森生物股份有限公司 | Capillary electrophoresis detection system and detection method |
| CN105973859B (en) * | 2016-06-29 | 2019-01-29 | 山东科立森生物股份有限公司 | Capillary electrophoresis detection system and detection method |
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