CN206930588U - A kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function - Google Patents
A kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function Download PDFInfo
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- CN206930588U CN206930588U CN201720686975.3U CN201720686975U CN206930588U CN 206930588 U CN206930588 U CN 206930588U CN 201720686975 U CN201720686975 U CN 201720686975U CN 206930588 U CN206930588 U CN 206930588U
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- delayed fluorescence
- excitation source
- fluorescence instrument
- photon
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Abstract
A kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function, including excitation source blue led and optical filtering elements；The excitation source power amplification circuit that the excitation source blue led connection is connected by MCU units, the MCU units connect PC input/output interface and high-precision photon electric pulse numbered card respectively；The optical filtering elements set high sensitivity photon-sensitive element backwards to the direction of sample；The high sensitivity photon-sensitive element is connected with the high-precision photon electric pulse numbered card.The utility model can detect the delay chlorophyll fluorescence that photosynthetical system II is sent, especially there is multiple pulses arousal function, and the wirelessly collection of control delay chlorophyll fluorescence and the transmission of data, testing tool can be provided to study plant physiology and perceiving environment using photosynthetical system II delay chlorophyll fluorescences.
Description
Technical field
The utility model patent is related to chlorophyll fluorescence detection technique field, swashs more particularly, to one kind with multiple pulses
Send out photosynthetical system II (PSII) delayed fluorescence instrument of function.
Background technology
For plant in photosynthesis, chlorophyll molecule can transit to upper state, upper state after absorbing the energy of a photon
Electronics it is unstable, may release energy in the form of heat；May also directly it be discharged in the form of fluorescence, here it is usual institute
The chlorophyll fluorescence or quick chlorophyll fluorescence said；The electronics of upper state may also be propagated forward final on electron transport chain
It is used to reduce carbon dioxide to synthesize energy.After the closing of extraneous exciting light, quick chlorophyll fluorescence disappears quickly, but
It is to have many electronics on electron transport chain, the energy of these electronics may return back broadcast, and excite PSII chlorophyll molecules again
The ability for producing chlorophyll fluorescence is made it have, because the passback speed of electronics is very slow, after exciting light closing, PSII leaves are green
Fluorescence caused by plain molecule can last very long, even up to several minutes or a few hours, this chlorophyll fluorescence is referred to as
Postpone chlorophyll fluorescence, abbreviation delayed fluorescence.
Because the transmission speed of electronics is related to the physiology of plant and environmental factor, therefore delay chlorophyll fluorescence can be made
The detection to plant physiology and environment is realized for a sensing technology.Compared with quick chlorophyll fluorescence, postpone chlorophyll fluorescence
Signal will not be excited light pollution due to being determined after exciting light closing, but because its intensity is very low, be surveyed
Examination get up it is relatively difficult, common chlorophyll fluorescence instrument can not realize the measurement to delayed fluorescence.
It is low to postpone chlorophyll fluorescence instrument flexibility by PSII in the market, it is difficult to realizes and a sample is repeated several times automatically
Measure or remote control measurement.Simultaneously because the data of delayed fluorescence can not collect during light excites, therefore obtain
Experimental data is relatively single.In order to improve the serviceability of delayed fluorescence, the delayed fluorescence with abundant dynamic characteristic is obtained very
It is necessary.Because the initial equilibrium state response that the pulse excitation that length does not wait comes out can provide extra pact for the parameter identification of system
Beam, therefore, in order to improve the richness of experimental data, it is necessary to excited using multiple pulses, especially every time after measurement
Automatic switchover pulse length.Therefore photosynthetical system II delayed fluorescence instrument of the design with multiple pulses arousal function is with important
Commercial value and scientific meaning.
Utility model content
In view of the above-mentioned problems existing in the prior art, the applicant provides a kind of light with multiple pulses arousal function
Syzygy system II delayed fluorescence instrument.The utility model can detect the delay chlorophyll fluorescence that photosynthetical system II is sent, and especially have
There are multiple pulses arousal function, and the wirelessly collection of control delay chlorophyll fluorescence and the transmission of data, can
For research plant physiology and postpone chlorophyll fluorescence perception environment using photosynthetical system II and testing tool is provided.
The technical solution of the utility model is as follows：
A kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function, including excitation source blue led with
And optical filtering elements；
The excitation source power amplification circuit that the excitation source blue led connection is connected by MCU units, the MCU are mono-
Member connects PC input/output interface and high-precision photon electric pulse numbered card respectively；
The optical filtering elements set high sensitivity photon-sensitive element backwards to the direction of sample；The high sensitivity light
Sub- sensing element is connected with the high-precision photon electric pulse numbered card；
The excitation source blue led and optical filtering elements are connected by optical fiber with the sample respectively.
The optical filtering elements pass through wavelength>680nm.
USB interface, network interface are provided with the MCU units.
The software of the collection of delay leaf green fluorescence and excitation source group intensity can be controlled by being equipped with the MCU units, real
Now postpone chlorophyll fluorescence location parameter to set, data preserve and display function.
The input/output interface includes touch screen displays, USB3 interfaces, the RS232 serial ports of wireless communication module.
Preferably, the optical fiber is the optical fiber of top bifurcated, and one top is connected to excitation source blue led, another
Individual top is connected to optical filtering elements, and its tail end is connected to sample.
Delayed fluorescence instrument described in the utility model also includes wireless communication module；The wireless communication module with it is described defeated
Enter output interface to be connected.
The wireless communication module is connected by wireless network with mobile terminal device.
Preferably, the mobile terminal device includes smart mobile phone, tablet personal computer.
In above-mentioned functional part, optical filtering elements can prevent exciting light from arriving at high sensitivity photon-sensitive element, right
High sensitivity photon sensing unit plays a protective role；
High sensitivity photon sensing unit can realize photoelectric conversion, and delay chlorophyll fluorescence photon is perceived；
Photon electric pulse numbered card can be carried out to pulse signal caused by photon excitation high sensitivity photon sensing unit
Count, so as to reach the purpose to PSII delay chlorophyll fluorescence tests；
Excitation source power amplification circuit provides energy for LED light source, while under MCU control, realizes to exciting light
The intensity and light application time in source are controlled；
MCU units are used for the works such as coordination is shown, control excitation source intensity, the data of delay chlorophyll fluorescence intensity preserve
Make；
PC is connected with input/output interface, can transfer data in PC, at the same can also control MCU units with
The data communication of wireless communication module.
Wireless communication module connects wireless device and MCU unit by input/output interface, supports communication, collection and remote
Thread management function；The terminal device of wireless communication module, such as smart mobile phone, order control MCU units and system software are sent, from
And realize in distal end arrange parameter, data are preserved, are shown and to functions such as excitation source controls.
The utility model is beneficial to be had technical effect that：
The utility model can be automatic at set time intervals by delay chlorophyll fluorescence that photosynthetical system II is sent
Take multiple measurements and remote collection, control and Data Transmission Controlling, and the pulse time length excited every time can be different.
PSII provided by the utility model postpones chlorophyll fluorescence instrument system high sensitivity, it is possible to achieve single photon is measured, can
The work of the delay chlorophyll fluorescence instrument is controlled in a manner of by wireless telecommunications.
Brief description of the drawings
Fig. 1 is the structure principle chart of the utility model PSII delayed fluorescence examining systems；
Fig. 2 is the block diagram of one embodiment of the present utility model；
Fig. 3 is the PSII delayed fluorescence test flow charts of the utility model one embodiment.
Embodiment
With reference to specific embodiments and the drawings, the utility model is described in further detail, embodiment of the present utility model
Including but not limited to example below.The utility model obviously can be implemented with the other manner that is described different from this, because
This should not limit scope of protection of the utility model with this embodiment content.
As depicted in figs. 1 and 2, a kind of photosynthetical system II delays with multiple pulses arousal function that the application provides are glimmering
Light instrument, including excitation source blue led 101 and optical filtering elements 105；The optical filtering elements 105 pass through wavelength
>680nm。
The excitation source blue led 101 connects the excitation source power amplification circuit 102 connected by MCU units 103,
The MCU units 103 connect high-precision photon electric pulse numbered card 104 and connect PC 109 by input/output interface 107；
The optical filtering elements 105 set high sensitivity photon sensing member 106 backwards to the direction of sample；It is described highly sensitive
Degree photon-sensitive element 106 is connected with the high-precision photon electric pulse numbered card 104；
The excitation source blue led 101 and optical filtering elements 105 pass through optical fiber 110 and the sample phase respectively
Even.
USB interface, network interface are provided with the MCU units 103, being also equipped with can control delay leaf green fluorescence to adopt
The software of collection and excitation source group intensity, realize that delay chlorophyll fluorescence location parameter is set, data preserve and display function.
The input/output interface 107 includes touch screen displays, USB3 interfaces, the RS232 serial ports of wireless communication module.
The optical fiber is the optical fiber of top bifurcated, and one top is connected to excitation source blue led 101, another top
End is connected to optical filtering elements 105, and its tail end is connected to sample.
The utility model can also include wireless communication module 108；The wireless communication module 108 and the input and output
Interface 107 is connected.The wireless communication module 108 is connected by wireless network with terminal device.The terminal device includes
Smart mobile phone, tablet personal computer etc..
The application method of this delayed fluorescence instrument is as shown in Figures 2 and 3：
(1) the optical fiber pool side of this delayed fluorescence instrument can be placed in a darkroom, can also other modes isolated blade
And photo-sensitive cell contacts with external light source.
(2) burst length and the length sequences of exciting light are set in the software control interface being installed on MCU103, with
And the number and the automatic nomenclature rule of file for wanting circulating repetition to test.
(3) excitation source power amplification circuit 102 is controlled using MCU units 103, closely drives excitation source blue led
101.LED light enters the pool side of optical fiber 110 by one end of fiber break, and then arrives at leaf, is sent out according to the time of regulation
Go out after exciting light just automatic distinguishing.
(4) after LED extinguishings, the delay chlorophyll fluorescence that leaf is sent is transferred to another point from the pool side of optical fiber
Zhi Duan, delayed fluorescence are transferred to optical filtering elements 105 to arrive at (such as the optional Channel of photon-sensitive element 106
Multiplier Tube, CMT), because now excitation source blue led 101 has extinguished, optical filtering elements 105 are at this
The individual stage does not play a role substantially, but during exciting light is opened, optical filtering elements 105 can prevent exciting light from arriving at height
The photon-sensitive element 106 of sensitivity, to protect the element.
(5) under the control of MCU units 103, optical signal is just converted to telecommunications by high sensitivity photon-sensitive element 106
Number, converting photons arrive at photon electric pulse numbered card 104 (such as optional PMS 400- photon countings card) for the pulse of electric signal,
And the umber of pulse proportional to fluorescence intensity is counted, and the PC 109 being connected with MCU units 103 can be passed data to.
(6) can enter in mobile terminal 111 by wireless network with being connected with wireless communication module 108 with MCU units 103
Row communication, can be set measurement parameter on mobile terminals, pass to the signal of MCU units 103 after system software therein processing,
Excitation source power amplification circuit 102 is controlled, complete pair delay chlorophyll fluorescence is automatic at set time intervals
Taking multiple measurements for task.PC 109 can also be communicated with, and control collection and the downloading data to PC 109 of fluorescence data.
Claims (9)
1. a kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function, it is characterised in that blue including excitation source
Color LED and optical filtering elements；
The excitation source power amplification circuit that the excitation source blue led connection is connected by MCU units, the MCU units point
Not Lian Jie PC input/output interface and high-precision photon electric pulse numbered card；
The optical filtering elements set high sensitivity photon-sensitive element backwards to the direction of sample；The high sensitivity photon sense
Element is answered to be connected with the high-precision photon electric pulse numbered card；
The excitation source blue led and optical filtering elements are connected by optical fiber with the sample respectively.
2. delayed fluorescence instrument according to claim 1, it is characterised in that the optical filtering elements pass through wavelength>
680nm。
3. delayed fluorescence instrument according to claim 1, it is characterised in that be provided with USB interface, network on the MCU units
Interface.
4. delayed fluorescence instrument according to claim 1, it is characterised in that delay can be controlled by being equipped with the MCU units
Leaf green fluorescence gathers and the software of excitation source group intensity, realizes that delay chlorophyll fluorescence location parameter is set, data preserve with
Display function.
5. delayed fluorescence instrument according to claim 1, it is characterised in that the input/output interface include touch screen displays,
The RS232 serial ports of USB3 interfaces, wireless communication module.
6. delayed fluorescence instrument according to claim 1, it is characterised in that the optical fiber is the optical fiber of top bifurcated, one
Top is connected to excitation source blue led, and another top is connected to optical filtering elements, and its tail end is connected to sample.
7. delayed fluorescence instrument according to claim 1, it is characterised in that also including wireless communication module；The radio communication
Module is connected with the input/output interface.
8. delayed fluorescence instrument according to claim 7, it is characterised in that the wireless communication module by wireless network with
Mobile terminal device is connected.
9. delayed fluorescence instrument according to claim 8, it is characterised in that the mobile terminal device includes smart mobile phone, put down
Plate computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720686975.3U CN206930588U (en) | 2017-06-13 | 2017-06-13 | A kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720686975.3U CN206930588U (en) | 2017-06-13 | 2017-06-13 | A kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function |
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| CN206930588U true CN206930588U (en) | 2018-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720686975.3U Expired - Fee Related CN206930588U (en) | 2017-06-13 | 2017-06-13 | A kind of photosynthetical system II delayed fluorescence instrument with multiple pulses arousal function |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110243798A (en) * | 2019-07-19 | 2019-09-17 | 江南大学 | Chlorophyll fluorescence instrument |
| CN115802519A (en) * | 2022-12-23 | 2023-03-14 | 江南大学 | Wireless chlorophyll fluorescence measurement system based on LORA networking |
-
2017
- 2017-06-13 CN CN201720686975.3U patent/CN206930588U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110243798A (en) * | 2019-07-19 | 2019-09-17 | 江南大学 | Chlorophyll fluorescence instrument |
| CN115802519A (en) * | 2022-12-23 | 2023-03-14 | 江南大学 | Wireless chlorophyll fluorescence measurement system based on LORA networking |
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Granted publication date: 20180126 Termination date: 20200613 |