CN102305778A - Micro-multispectral fluorescence reception and treatment system - Google Patents
Micro-multispectral fluorescence reception and treatment system Download PDFInfo
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- CN102305778A CN102305778A CN201110128960A CN201110128960A CN102305778A CN 102305778 A CN102305778 A CN 102305778A CN 201110128960 A CN201110128960 A CN 201110128960A CN 201110128960 A CN201110128960 A CN 201110128960A CN 102305778 A CN102305778 A CN 102305778A
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Abstract
The invention discloses a micro-multispectral fluorescence reception and treatment system. The micro-multispectral fluorescence reception and treatment system comprises an excitation unit, a fluorescence collection unit and a data processing unit, wherein the excitation unit comprises an excitation light source, an exciting light filter and an optical convergence component; the fluorescence collection unit comprises a photoelectric converter and a micro-optical filter which is attached to the photoelectric converter closely; and the micro-optical filter is a highly integrated multi-channel optical filter. An object to be imaged radiates the fluorescence with a specific wavelength under the excitation of irradiation light of the excitation unit; the optical convergence component converges the fluorescence; then the converged fluorescence is irradiated to the photoelectric converter through the micro-optical filter; the photoelectric converter acquires corresponding image information according to a fluorescence signal; and the data processing unit analyzes and processes the image information and outputs a result. The system can collect multiple fluorescence signals of stimulated radiation and acquire image information in a high-efficiency and low-cost way, and can accurately and objectively reflect detection results in real time.
Description
Technical field
The present invention relates to biochemistry detection and external medical diagnostic equipment, relate in particular to a kind of little multispectral fluorescence and receive and disposal system.
Background technology
At present, the employed instrument of medical institutions' clinical diagnosis and biological detection, the fluorescence acquisition system of employing is a characteristic with PCR (Picymerase Chain Reaction) PCR all.In the PCR reaction system, add the primer that contains fluorescent material; Fluorescent material sends the light of another feature wavelength under the excitation source irradiation of specific wavelength; Detect the light intensity that fluorescent material is launched in the PCR course of reaction through photomultiplier acceptance, optical fiber optical signal transmission, discrete optical filter beam split, monitoring pcr amplification reaction process.Draw real-time analytic curve through computer software analysis, calculate the Ct value, provide the nucleic acid concentration value of sample starting template, carry out the detection and the genetic analysis of the various pathogen of DNA/RNA.
It is discrete optical filter beam split that existing clinical diagnosis, biochemical instrument adopt the method for designing of fluorescence gathering system; The optical fiber optical signal transmission; Photomultiplier is accepted fluorescence signal and is carried out opto-electronic conversion; Calculate original gene content and expression formula through preposition amplification and data processing post analysis, detect the situation of sample tissue element variation.This project organization cost is high, and the collection of light and opto-electronic conversion are complicated, the poor stability that authentic specimen information is obtained, and measurable number of samples is restricted.
Adopt the common photoelectric multiplier tube; Be to convert faint optical signal to electric signal; But when illumination is mapped to photomultiplier; Photocathode inspires photoelectron in vacuum; Photoelectron is pressed the focusing board electric field and is got into dynode system; Obtain multiplication through further Secondary Emission and amplify, the electronics after the amplification is collected with anode and is exported as signal.Owing to adopted the Secondary Emission dynode system; So photomultiplier has higher sensitivity in the photodetection of the emittance of ultraviolet, visible light and near-infrared region, in the clinical diagnosis of carrying out optical measurement and spectral analysis and biochemical instrument, be widely used.But the common photoelectric multiplier tube once can only be measured an information, and passage has only one, has limited the measurement of multi-channel information; Photomultiplier in actual use, detection sensitivity reduces because of strong illumination or because of irradiation time is long, stops after the irradiation again part and recovers, and " tired " phenomenon occurs; The sensitivity of photomultiplier photoanode surface each point is inhomogeneous.Photomultiplier also needs accessory circuit such as high-voltage power supply, amplification and Discr., cool room, magnetic shielding, base etc. in addition, and this just makes that the volume of total system is big, and is high to environment requirement.
In recent years, along with development of semiconductor, multi-anode photomultiplier and silicon photomultiplier are come out one after another, and its common characteristic is to make many very thin photomultipliers form matrix, increases detection channels, reduces the volume of photomultiplier; Optical detection unit adopts the optical fiber lens arra.But the anode end of multi-anode photomultiplier is subjected to the restriction of fine wire, and it is measured passage and can only accomplish hundred at present.The optical fiber that is used for optical detection unit, for sample carriers, owing to be point measurement, the real information of the representative tested sample that the light signal of detection and transmission can not be accurately complete.Aspect the fixed form of optical fiber: optical fiber is fixed on the bottom of sample, if the location will definitely not influence the detection quality of signal; Optical fiber is fixed on the top of sample, and in the experimentation, what microwell plate can be frequent moves, and not only influences the quality of measurement but also brings inconvenience to the operator, optical fiber contaminated accuracy that also can reduce measurement in experimentation simultaneously.
Adopt discrete optical filter beam split; In a large amount of experimentations; Need frequent adjustment optical filter wheel to make discrete optical filter beam split different wavelengths; So not only can produce artificial noise; Can cause the offset of obtaining image because the machinery of optical filter wheel moves simultaneously, the quality of image information is obtained in influence.
Summary of the invention
The technical matters that the present invention mainly solves provides a kind of little multispectral fluorescence and receives and disposal system, overcome existing fluorescence receive with processing unit can not be in real time, the defective of the real information that obtains fluorescence signal of accurate, low cost, miniature multi-channel.
To solve the above problems, the present invention uses a technical solution is: to provide a micro multispectral fluorescence receiving and processing system, comprising: means for generating monochromatic light to be irradiated uniformly with the imaged object to be imaged in the object to excite fluorescence excitation radiation unit , the object to be imaged for collecting the fluorescence radiation and the fluorescence image information into the collection unit and a fluorescence conversion in accordance with image information analysis and processing of the data processing unit; said phosphor together collecting unit includes an optical member, photoelectric converter and adapter affixed to the photoelectric converter input terminal of the micro filter, the micro-filter is a highly integrated multi-channel filter, said micro-filter contains a plurality of closely spaced micro-optical element, said optical micro- element are arranged in a matrix, wherein a micro-optical element covering one of said photoelectric photoelectric conversion unit; together member is provided on the optical object to be imaged in the optical path of the fluorescence radiation, said micro-filter provided in the the optical path of the reflected light gathering member, connected to an output terminal of the photoelectric converter of the data processing unit; unit emits the excitation light irradiated on the object to be imaged, the object to be imaged by the excitation radiation of a specific wavelength of the fluorescence, together fluorescent light on the optical member, the optical member of the fluorescent converge together to a micro filter and the close contact with the micro-filter on the photoelectric conversion device, the micro filter allows the fluorescence signal through a specific wavelength, fluorescence signal through the micro-filter is irradiated on the photoelectric conversion device, the photoelectric conversion device according to the fluorescent signal to obtain the corresponding image information and the image information to said data processing unit, a data processing unit for transmission to the photoelectric converter calibration information, analysis and processing and outputs the analysis result of the processing.
Wherein, the photoelectric commutator of said phosphor collection unit is CCD or cmos image sensor, and said micro optical filter is integrated on CCD or the cmos image sensor.
Wherein, the photoelectric commutator of said phosphor collection unit is for to press the compact arranged photomultiplier of array, and micro optical filter is integrated on the photomultiplier of little gust of arrangement.
Wherein, the said unit that excites comprises LASER Light Source and convex reflecting mirror, and said convex reflecting mirror is arranged on the emitting light path of LASER Light Source, and the said imaging object of treating is arranged on the reflected light path of convex reflecting mirror.
Wherein, the said unit that excites comprises arrowband high-power LED light source and exciting light optical filter, is radiated at equably behind the emergent light process exciting light optical filter of said arrowband high-power LED light source and treats on the imaging object.
Wherein, The said unit that excites comprises arrowband high-power LED light source, exciting light optical filter and plane mirror; The emergent light of said arrowband high-power LED light source shines said plane mirror through the exciting light optical filter, and the said imaging object of treating is positioned on the reflected light path of said plane mirror.
Wherein, it is concave mirror that said optics converges parts, and said micro optical filter and integrated with it photoelectric commutator are arranged on the focus of said concave mirror.
The invention has the beneficial effects as follows: be different from the clinical diagnosis of prior art; Fluorescence in the biochemical instrument receives and disposal system adopts photomultiplier to accept; The optical fiber optical signal transmission; Discrete optical filter beam split makes image inaccurate; Structure is huge; High and the testable limited amount of cost; Little multispectral fluorescence of the present invention receives and disposal system adopts miniature multispectral technology; Utilize micro optical filter that the fluorescence signal of the radiation that is stimulated of collection is filtered; Micro optical filter is highly integrated multi-channel filter; Be close to the input end of photoelectric commutator; Arrowband fluorescence signal corresponding to the primary fluorescence specific wavelength obtains corresponding image through being radiated at photoelectric commutator from the microchannel of micro optical filter, handle back output result through data processing unit.In the present invention; The fluorescence signal of the biochemical reaction process that is used for reporting that the fluorescent dye sample takes place; Become real-time little multispectral image transmission on the whole; And then the photoelectric commutator that is had micro optical filter is noted; The image that is obtained accurately, sensitive, reflect the Biochemical Information of each test tube sample carrier in the micropore template objectively, improved the signal to noise ratio (S/N ratio) and the reliability that detects sample of image.Only need the miniature narrow band pass filter of adjustment to allow just to go for the fluorescence reception of different series and the clinical diagnosis and the biochemical instrument of processing procedure through light wavelength.For example biological detection and clinical diagnosis instruments such as fluorescent colloid detector, fluorescent polyase chain reaction appearance, immune time resolution detector, immunochemiluminescence detector.The present invention makes instrument easily; The mode of collecting fluorescence designs very compact, succinct; Be easy to standardization simultaneously; Reliability of products reaches high standard easily; Quality in the instrument manufacture process is controlled easily; In the manufacturing of product, reduced cost, the complexity of design difficulty and instrument reduces significantly.
Description of drawings
Fig. 1 is the light path principle figure of one embodiment of the invention;
Fig. 2 is the structured flowchart of embodiment among Fig. 1 of the present invention;
Fig. 3 is the structural representation of micro-filtration wave plate of the present invention;
Fig. 4 is an A place partial enlarged drawing among Fig. 3;
Fig. 5 is the light path principle figure that the present invention uses LASER Light Source;
Fig. 6 is the index path that the present invention uses the arrowband high-power LED light source;
Fig. 7 is the index path that the present invention uses another embodiment of arrowband high-power LED light source.
Embodiment
By describing technology contents of the present invention, structural attitude in detail, realized purpose and effect, give explanation below in conjunction with embodiment and conjunction with figs. are detailed.
See also Fig. 1 and Fig. 2, the present invention provides a kind of little multispectral fluorescence to receive and disposal system, and comprising: excite unit 1, photoelectric conversion unit 2 and data processing unit 3, Sheng is loaded with fluorescent dye 4 and treats that promptly the test tube of imaging object is placed on the micropore template 5; Excite unit 1 to launch the monochromatic light uniform irradiation on the fluorescent dye 4 of micropore module 5, fluorescent dye 4 is stimulated and gives off the fluorescence of specific wavelength.Said phosphor collection unit 2 comprises that optics converges parts 23, photoelectric commutator 22 and is close to the micro optical filter 21 of photoelectric commutator 22 input ends; Said micro optical filter 21 is highly integrated multi-channel filter; Optics converges parts 23 and is arranged on the light path of fluorescent dye 4 fluorescent radiations; Said micro optical filter 21 is arranged on the reflected light path that said optics converges parts 23, and the output terminal of said photoelectric commutator 22 connects said data processing unit 3.
Among the present invention; The light that excites unit 1 to launch is radiated on the fluorescent dye 4; Fluorescent dye 4 is stimulated and gives off the fluorescence of intrinsic specific wavelength; Fluorescence is radiated at said optics and converges on the parts 23; Optics converges parts 23 fluorescence is converged on the micro optical filter 21; Fluorescence signal is radiated at after by micro optical filter 21 on the optical-electrical converters of closely placing with micro optical filter 21 22; In the present embodiment; Optics converges parts 23 and adopts concave mirror; Micro optical filter 21 and be arranged on the focus of concave mirror with optical-electrical converters 22 that micro optical filter 21 is closely placed; The fluorescence of fluorescent dye 4 radiation is radiated on the concave mirror; Concave mirror converges fluorescence according to optical principle and increases light intensity; The fluorescence that reflects impinges upon on the micro optical filter 21; Said micro optical filter 21 is highly integrated multi-channel filter; Only allow the fluorescence signal of specific wavelength to pass through; Optical-electrical converter 22 obtains corresponding image information according to fluorescence signal; And flowing to data processing unit 3, the result of analyzing and processing is demarcated, analyzes and handled and export to the information that 3 pairs of said optical-electrical converters 22 of data processing unit send.Said data processing unit 3 can be Computerized image processing system; The image information that said photoelectric commutator 22 obtains is delivered to demarcation and the processing that Computerized image processing system carries out image through the computer interface circuit; Fluorescent dye 4 is carried out real-time analysis and detection by quantitative, and the result of analyzing and processing is transported on the graphoscope shows.
Be different from the clinical diagnosis of prior art; Fluorescence in the biochemical instrument receives and disposal system adopts photomultiplier to accept; The optical fiber optical signal transmission; Discrete optical filter beam split makes image inaccurate; Structure is huge; High and the testable limited amount of cost; Little multispectral fluorescence of the present invention receives and disposal system adopts miniature multispectral technology; Utilize micro optical filter that the fluorescence signal of the radiation that is stimulated of collection is filtered; Micro optical filter is highly integrated multi-channel filter; Be close to the input end of photoelectric commutator; Arrowband fluorescent signal corresponding to the primary fluorescence specific wavelength obtains corresponding image through being radiated at photoelectric commutator from the microchannel of micro optical filter, handle back output result through data processing unit.In the present invention; The fluorescence signal of the biochemical reaction process that is used for reporting that the fluorescent dye sample takes place; Become real-time little multispectral image transmission on the whole; And then the photoelectric commutator that is had micro optical filter is noted; The image that is obtained accurately, sensitive, reflect the Biochemical Information of each test tube sample carrier in the micropore template objectively, improved the signal to noise ratio (S/N ratio) and the reliability that detects sample of image.Only need the miniature narrow band pass filter of adjustment to allow just to go for the fluorescence reception of different series and the clinical diagnosis and the biochemical instrument of processing procedure through light wavelength.For example biological detection and clinical diagnosis instruments such as fluorescent colloid detector, fluorescent polyase chain reaction appearance, immune time resolution detector, immunochemiluminescence detector.The present invention makes instrument easily; The mode of collecting fluorescence designs very compact, succinct; Be easy to standardization simultaneously; Reliability of products reaches high standard easily; Quality in the instrument manufacture process is controlled easily; In the manufacturing of product, reduced cost, the complexity of design difficulty and instrument reduces significantly.
Consult Fig. 3; Micro optical filter 21 of the present invention comprises a plurality of compact arranged optical filtering infinitesimals 211; Said optical filtering infinitesimal 211 is arranged; The size of said optical filtering infinitesimal must not be significantly less than the single Pixel Dimensions of pairing said photoelectric commutator 22, the photoelectric conversion unit that a said optical filtering infinitesimal 211 covers said photoelectric commutator 22.The arrowband fluorescent signal of primary fluorescence specific wavelength forms images on photoelectric commutator 22 through optical filtering infinitesimal 211; Between pixel and the pixel; Automatically calibrating aligns between image and the image; Make the image that obtained accurately, sensitive, reflect the Biochemical Information of each test tube sample carrier in the micropore template objectively, improved the signal to noise ratio (S/N ratio) and the reliability that detects model of image.The light wavelength that said optical filtering infinitesimal allows to pass through can be set to identical or different, and for example in Fig. 4, it is that the light of λ 1, λ 2, λ 3 and λ 4 passes through that four optical filtering infinitesimals can allow wavelength respectively, detects when can realize different wave length fluorescence like this.
In one embodiment; The photoelectric commutator 22 of said phosphor collection unit 2 is CCD or cmos image sensor; Said micro optical filter 21 is integrated on CCD or the cmos image sensor; Be specially micro optical filter 21 directly is produced on the opto-electronic conversion sensitive element of a CCD/COMS monochrome image sensor with low light level imaging capability;, form images on the imaging surface of CCD/COMS monochrome image sensor through behind the micro optical filter 21 corresponding to the fluorescent signal of primary fluorescence specific wavelength with low light level imaging capability.Said photoelectric commutator 22 adopts CCD or COMS imageing sensor, and the CCD or the cmos image sensor that are integrated with micro optical filter 21 just become multispectral CCD/COMS camera or video camera.
In another embodiment, said photoelectric commutator 22 is for to press the compact arranged photomultiplier of array, and said micro optical filter 21 is integrated in this by on the compact arranged photomultiplier of array.Said micro optical filter 21 directly is produced on the photosensor chip with micro production mode (like vacuum coating and microlithography technology etc.), and is integrated in the photomultiplier transit tube module.
Consult Fig. 5; In one embodiment; Laser cell 1 comprises LASER Light Source 11 and convex reflecting mirror 14; Convex reflecting mirror 14 is arranged on the emitting light path of LASER Light Source 11; The rayed that LASER Light Source 11 is sent is on convex reflecting mirror 14, and light is radiated on the fluorescent dye 4 after convex reflecting mirror 14 reflections equably.In this embodiment; The light that LASER Light Source 11 is sent expands bundle through convex reflecting mirror 14; Convex reflecting mirror 14 has the characteristic to the incident light scattering; Enlarged catoptrical range of exposures like this, reflected light shines the 5 last times all standing uniformly of micropore template and shines each fluorescent dye 4 on the micropore template 5.
Consult Fig. 6, in another embodiment, excite unit 1 to comprise arrowband high-power LED light source 11a and exciting light optical filter 12, be radiated at equably on the fluorescent dye 4 behind the emergent light process exciting light optical filter 12 of said arrowband high-power LED light source 11a.Fluorescent dye 4 gives off fluorescence and converges parts 23 through optics and converge reinforcement, forms images on photoelectric commutator 22 through micro optical filter 21 again.Imaging system 40 comprises that fluorescent dye 4 and optics converge parts 23 among Fig. 6, and structure that it is concrete and light path are above existing to be specifically described, and here to its simplification, no longer specifically describes.Hereinafter the imaging system of Chu Xianing is represented identical implication.
Fig. 7 has provided another embodiment as excitation source with arrowband high-power LED light source 11a; In this embodiment; Excite unit 1 to comprise arrowband high-power LED light source 11a, exciting light optical filter 12 and plane mirror 13; The emergent light of arrowband high-power LED light source 11a incident angle with 45 ° after exciting light optical filter 12 filters is radiated on the plane mirror 13; The reflected light of plane mirror 13 shines on the imaging system 40, and the emergent light of imaging system 40 is radiated at imaging on the photoelectric commutator 22 through micro optical filter 21 again.When reality is used; Reasonably adjust the angle of plane mirror 13 according to the position relation of arrowband high-power LED light source 11a, micropore module 5; Make rayed that arrowband high-power LED light source 11a sends on plane mirror 13; Fluorescent dye 4 is positioned on the reflected light path of plane mirror 13, and the reflection that the light that arrowband high-power LED light source 11a sends can pass through plane mirror 13 evenly covers fluorescent dye and gets final product.
Among the present invention, the light source that produces exciting light is except can also using high-pressure sodium lamp, quartz-halogen-tungsten lamp or the conduct of LED laser instrument of co-wavelength scope to substitute for the LASER Light Source and arrowband high-power LED light source like xenon lamp and so on.Its embodiment repeats no more.
The above only is embodiments of the invention; Be not so limit claim of the present invention; Every equivalent structure or equivalent flow process conversion that utilizes instructions of the present invention and accompanying drawing content to be done; Or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (7)
1. little multispectral fluorescence receives and disposal system, it is characterized in that, comprising:
Be used to produce the monochromatic light uniform irradiation treat imaging object with excite treat the imaging object radiofluorescence excite the unit, be used to collect the fluorescence of treating the imaging object radiation and be converted into the phosphor collection unit of image information and to carry out the data processing unit of analyzing and processing according to the image information of fluorescence conversion;
Said phosphor collection unit comprises that optics converges parts, photoelectric commutator and is close to the micro optical filter of photoelectric commutator input end; Said micro optical filter is highly integrated multi-channel filter; Said micro optical filter comprises a plurality of compact arranged optical filtering infinitesimals; Said optical filtering infinitesimal is arranged, and a said optical filtering infinitesimal covers a photoelectric conversion unit of said photoelectric commutator; Said optics converges parts and is arranged on the light path of treating the imaging object fluorescent radiation, and said micro optical filter is arranged on the reflected light path that said optics converges parts, and the output terminal of said photoelectric commutator connects said data processing unit;
The said light that excites the unit to launch is radiated to be treated on the imaging object; Treating that imaging object is stimulated gives off the fluorescence of specific wavelength; Fluorescence is radiated at said optics and converges on the parts; Optics converge that parts converge to micro optical filter with fluorescence and the optical-electrical converter that fits tightly with micro optical filter on; Micro optical filter allows the fluorescence signal of specific wavelength to pass through; Fluorescence signal is radiated on the optical-electrical converter after by micro optical filter; Optical-electrical converter obtains corresponding image information according to said fluorescence signal; And sending image information to said data processing unit, data processing unit demarcates, analyzes and handle and export the result of analyzing and processing to the information that said optical-electrical converter sends.
2. little multispectral fluorescence according to claim 1 receives and disposal system, and it is characterized in that: the photoelectric commutator of said phosphor collection unit is CCD or cmos image sensor, and said micro optical filter is integrated on CCD or the cmos image sensor.
3. little multispectral fluorescence according to claim 1 receives and disposal system, and it is characterized in that: the photoelectric commutator of said phosphor collection unit is for to press the compact arranged photomultiplier of array, and micro optical filter is integrated on the photomultiplier of little gust of arrangement.
4. little multispectral fluorescence according to claim 1 receives and disposal system; It is characterized in that: the said unit that excites comprises LASER Light Source and convex reflecting mirror; Said convex reflecting mirror is arranged on the emitting light path of LASER Light Source, and the said imaging object of treating is arranged on the reflected light path of convex reflecting mirror.
5. little multispectral fluorescence according to claim 1 receives and disposal system; It is characterized in that: the said unit that excites comprises arrowband high-power LED light source and exciting light optical filter, is radiated at equably behind the emergent light process exciting light optical filter of said arrowband high-power LED light source and treats on the imaging object.
6. little multispectral fluorescence according to claim 1 receives and disposal system; It is characterized in that: the said unit that excites comprises arrowband high-power LED light source, exciting light optical filter and plane mirror; The emergent light of said arrowband high-power LED light source shines said plane mirror through the exciting light optical filter, and the said imaging object of treating is positioned on the reflected light path of said plane mirror.
7. receive and disposal system according to each described little multispectral fluorescence of claim 1-6; It is characterized in that: it is concave mirror that said optics converges parts, and said micro optical filter and integrated with it photoelectric commutator are arranged on the focus of said concave mirror.
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