CN111154641A - Full-automatic nucleic acid extraction amplifications detector - Google Patents
Full-automatic nucleic acid extraction amplifications detector Download PDFInfo
- Publication number
- CN111154641A CN111154641A CN202010098156.3A CN202010098156A CN111154641A CN 111154641 A CN111154641 A CN 111154641A CN 202010098156 A CN202010098156 A CN 202010098156A CN 111154641 A CN111154641 A CN 111154641A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- acid extraction
- detection device
- amplification
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The invention discloses a full-automatic nucleic acid extraction amplification detector, which comprises a working box body and an operation display screen, wherein a core device is arranged in the working box body and is electrically connected with the operation display screen, the core device comprises a nucleic acid extraction amplification detection device, a thermal circulation device, an adjustable light supplement lamp device and an optical detection device, the optical detection device is arranged in the core device and is positioned above the nucleic acid extraction amplification detection device, the nucleic acid extraction amplification detection device extracts, amplifies and detects nucleic acid by pressing a start button on the operation display screen, after detection is finished, the adjustable light supplement lamp device can automatically light up to assist the optical detection device to photograph and detect the nucleic acid extraction amplification detection device, the detection condition of a sample is displayed by operating the display screen, the whole process can be automatically carried out without manual interference, The automation degree is high, errors and cross contamination caused by manual detection are avoided, and the efficiency is greatly improved.
Description
Technical Field
The invention belongs to the field of molecular biology detection, and particularly relates to full-automatic nucleic acid extraction amplification detection.
Background
Nucleic acid is a biological macromolecular compound polymerized by a plurality of nucleotides, is one of the most basic substances of life, widely exists in all animal and plant cells and microorganisms, is usually combined with protein to form nucleoprotein in the organisms, is a carrier of genetic information, is the most important biological information molecule and is the main object of molecular biology research, so the extraction of the nucleic acid is the most important and most basic operation in molecular biology experimental technology, has good application prospect for epidemic prevention and control and epidemiological investigation, and is also very suitable for the fields of biological anti-terrorism, agriculture, food safety, biomedicine and the like.
Early experiments were performed by manual extraction and amplification, which takes a long time and the experimental results are easily affected by individual operation differences of experimenters, however, with the development of industry, some nucleic acid extraction and amplification detection devices are beginning to appear to solve the problem of experimental result differences. The existing nucleic acid extraction amplification detection equipment has some problems, the nucleic acid extraction amplification detection equipment is not automatic enough, multiple complex biological reaction steps of sample treatment, nucleic acid extraction, amplification, detection and the like need to be manually processed, the detection result is recorded, the detection efficiency is low, errors are easy to occur, most of templates used by the equipment need to be subjected to an independent sample extraction step, the step is easy to cause template cross contamination, and the judgment of the sample detection result is influenced, but the nucleic acid extraction amplification detection equipment generally has the problems of complex structure, need of using a fluorescent probe, high detection cost and the like.
Disclosure of Invention
In order to solve the problems of insufficient automation, high cost, manpower consumption, low error efficiency of detection results and the like of nucleic acid extraction amplification detection equipment in the prior art, the invention provides an instrument integrating the functions of nucleic acid extraction, amplification and detection, the process is fully automatic, manual interference is not needed, the efficiency, the accuracy and the operability of nucleic acid detection are effectively improved, and the human resources can be effectively saved
In order to solve the problems of the prior art, the invention adopts the following technical scheme:
a full-automatic nucleic acid extraction amplification detector comprises a work box body, a front door arranged in front of the work box body and an operation display screen, wherein the operation display screen is positioned above the front door, a machine core device is arranged in the working box body and is electrically connected with the operation display screen, the core device comprises a nucleic acid extraction amplification detection device for extracting, amplifying and detecting nucleic acid of a sample, a thermal cycle device for providing proper temperature for the motion of the nucleic acid extraction amplification detection device and the nucleic acid extraction amplification of the sample, an adjustable light supplement lamp device and an optical detection device, the thermal cycling device is arranged in the machine core device, the nucleic acid extraction amplification detection device is arranged above the thermal cycling device, the adjustable light supplement lamp device is arranged above the optical detection device, and the optical detection device is arranged in the core device and is positioned above the nucleic acid extraction amplification detection device;
the nucleic acid extraction, amplification and detection device comprises a sample adding hole and a plurality of holding tanks, wherein the holding tanks can be used for holding samples and extracting solutions such as nucleic acid in the samples;
one side of the nucleic acid extraction amplification detection device is provided with a power device, and one end of the power device is connected with one end of the nucleic acid extraction amplification detection device, so that the power device drives the nucleic acid extraction amplification detection device to move to a working position on the thermal cycle device;
an optical coupling sensor for sensing the movement position of the nucleic acid extraction amplification detection device is arranged above the thermal circulation device, the front door is opened, the sample is injected into the plurality of accommodating tanks through the sample adding hole to carry out the nucleic acid extraction, amplification and detection of the sample, the chromatographic membrane strip is used for chromatography and detection of the sample and the nucleic acid in the plurality of accommodating tanks, the operation display screen is provided with a start button, the start button is pressed, the power device moves the nucleic acid extraction amplification detection device to the position of the optical coupling sensor to carry out the resetting and nucleic acid extraction amplification detection work in the sample, after the nucleic acid extraction amplification detection device detects the nucleic acid in the sample, the adjustable light supplement lamp device automatically lights up so as to assist the optical detection device above the nucleic acid extraction amplification detection device to photograph and detect the chromatographic membrane strip, and the detection condition of the sample is displayed through the operation display screen, the automatic degree is high, avoids error and cross contamination caused by manual detection, greatly improves the efficiency, is not complicated in structure, and can effectively reduce the cost by detecting through an optical detection device without using a fluorescent probe.
Furthermore, the movement device comprises a nucleic acid detection and calibration device and a rotation driving device, the nucleic acid detection and calibration device is arranged above the nucleic acid extraction and amplification detection device so as to realize the close fit of the nucleic acid extraction and amplification detection device and the thermal cycling device, and the rotation driving device is positioned above the nucleic acid detection and calibration device and is movably connected with the nucleic acid detection and amplification device.
Furthermore, the power device comprises a first stepping motor and a first screw rod pair, the first screw rod pair is positioned at one end of the first stepping motor, one end of the first screw rod pair is connected with the first stepping motor, the other end of the first screw rod pair is connected with the nucleic acid extraction amplification detection device, and the first stepping motor drives the first screw rod pair to move the nucleic acid extraction amplification detection device to the position of the optical coupling sensor along the upper part of the thermal circulation device to reset or move to a working position.
Further, the nucleic acid extraction amplification detection device comprises a shell and a chassis, the chassis is positioned at the lower end of the shell and connected with the lower end of the shell to form a sealed space, the plurality of holding tanks comprise a sample tank, a combination tank, a magnetic bead tank, an amplification tank, a detection tank and a waste liquid tank, the combination tank and the magnetic bead tank are used for holding reactants and magnetic beads, a sample placed in the sample tank is cracked under the action of a thermal circulation device and is extracted with nucleic acid through the magnetic beads in the combination tank and the magnetic bead tank, the plurality of holding tanks are distributed on a circumferential curved surface of the chassis, a lifting rotating assembly is connected to the center of the chassis in an installing manner, a suction assembly is arranged on one side of the lifting rotating assembly, one side of the lifting rotating assembly is connected with the suction assembly to drive the suction assembly to lift and rotate so as to realize that the suction assembly can suck and discharge liquid between the plurality, the condition of cross contamination can be avoided effectively by setting up lifting and rotating assembly control suction assembly to switch between a plurality of holding tanks.
Further, the suction assembly is including carrying out imbibition and the imbibition barrel of flowing back, imbibition cavity, hose, the piston assembly of flowing back to solutions such as sample and sample nucleic acid in a plurality of holding tanks, the imbibition barrel is located the below of imbibition cavity and is connected with it, the hose is located between imbibition cavity and the piston assembly, the piston assembly is located one side of imbibition cavity, the imbibition cavity is connected through hose and piston assembly.
Furthermore, the piston assembly comprises a large piston cavity, an atmospheric pressure cavity, wherein the large piston cavity is positioned above the atmospheric pressure cavity and is connected with the atmospheric pressure cavity to form a large piston air pressure cavity system, the piston assembly further comprises a small piston cavity, a small air pressure cavity, the small piston cavity is positioned above the small air pressure cavity and is connected with the small air pressure cavity to form a small piston air pressure cavity system, a connecting sleeve is arranged between the large piston air pressure cavity system and the small piston air pressure cavity system, and the large piston air pressure cavity system and the small piston air pressure cavity system are connected through the connecting sleeve, so that the large piston cavity, the atmospheric pressure cavity, the small piston cavity, the small air pressure cavity and the connecting sleeve form a piston assembly.
Further, the top of piston assembly is equipped with atmospheric pressure flux thrust unit, atmospheric pressure flux thrust unit is including step-by-step drive subassembly and push rod subassembly, thereby step-by-step drive subassembly drive push rod subassembly promotes piston assembly reciprocating motion and realizes sucking the gas and then realize the imbibition barrel and absorb solution or to discharge solution in a plurality of holding tanks from a plurality of holding tank internal grooves to the imbibition cavity.
Furthermore, the push rod assembly comprises a small push rod and a large push rod, and the small push rod and the large push rod are arranged oppositely.
Further, step-by-step drive assembly is vice including fourth step motor, the fourth lead screw that is located the fourth step motor below, the vice one end and the fourth step motor of fourth lead screw are connected, the vice other end of fourth lead screw is connected with the little push rod, thereby drives the vice motion of fourth lead screw through fourth step motor and drives little push rod and promote little piston chamber and carry out the back and forth movement in little atmospheric pressure chamber, and then realizes that the imbibition barrel carries out the liquid imbibition to in a plurality of holding tanks through piston assembly.
Further, step-by-step drive assembly is still including fifth step motor, the fifth lead screw that is located the fifth step motor below is vice, the one end and the fifth step motor of fifth lead screw are connected, the other end and the big push rod of the vice fifth lead screw of fifth lead screw are connected, thereby drive the vice motion of fifth lead screw through fifth step motor and drive big push rod and promote big piston chamber and carry out the back and forth movement at atmospheric pressure intracavity, and then realize that the imbibition barrel carries out the flowing back through the liquid of piston assembly in to a plurality of holding tanks.
Furthermore, the nucleic acid detection and calibration device comprises a second stepping motor, a second screw rod pair and an elastic lifting platform, wherein the second screw rod pair is positioned below the second stepping motor, the second screw rod pair is arranged above the elastic lifting platform, one end of the second screw rod pair is connected with the second stepping motor, the other end of the second screw rod pair is connected with the elastic lifting platform, and the second stepping motor drives the second screw rod pair to move downwards so as to press the elastic lifting platform on the nucleic acid extraction and amplification detection device, so that the nucleic acid extraction and amplification detection device is tightly attached to the thermal circulation device.
Furthermore, the elastic lifting platform is provided with a compression spring, and the compression spring plays a role in preventing the elastic lifting platform from overshooting when being pressed on the nucleic acid extraction amplification detection device, so that the nucleic acid extraction amplification detection device is protected.
Further, the rotation driving device comprises a motor driving assembly, a first pinion and a first gearwheel, the first pinion is mounted below the motor driving assembly, the first gearwheel is mounted below the first pinion to form a rotation driving device which drives the lifting rotating assembly to rotate and lift, and the motor driving assembly drives the first pinion and the first gearwheel to be matched to drive the first pinion and the first gearwheel to automatically rotate.
Further, the lifting and rotating assembly comprises a screw rod, a connecting arm, a second pinion and a second gear wheel, one end of the connecting arm is sleeved on the screw rod, the other end of the connecting arm is connected with the liquid suction barrel through a liquid suction cavity, the second gear wheel is connected with the upper end of the screw rod, and the second pinion is mounted above the second gear wheel to lift the screw rod and further rotate the liquid suction barrel.
Further, the surface of shell is equipped with magnetic sensor, the outside extension of the linking arm one end of nucleic acid extraction amplification detection device is equipped with magnet, thereby rotate drive arrangement drive lift rotating assembly and rotate and go up and down to drive the magnet rotation and the lift on the linking arm and make magnet and magnetic sensor be in same level, vertical direction and then make nucleic acid extraction amplification detection device accomplish and reset.
Further, the adjustable light supplement lamp device comprises a light fixing frame, a light bar mounting seat, a light bar, a fixing shaft and a rotating shaft sleeve, the light bar mounting seat, the light bar, the fixed shaft and the rotating shaft sleeve are all positioned above the light fixing frame, the lamp strip is detachably arranged above the lamp strip mounting seat, the fixed shaft is positioned at one end of the lamp strip mounting seat, the rotating shaft sleeve is positioned at one side of the fixed shaft, one end of the fixed shaft is used for installing the light bar installation seat on the light fixing frame, the other end of the fixed shaft is connected with the rotating shaft sleeve, the light bar on the light bar installation seat is driven to rotate by the rotating shaft sleeve, thereby the regulation of the light of being convenient for has improved convenience and flexibility, can be convenient for put and detect the sample experimental conditions on the nucleic acid extraction amplification detection device, plays supplementary optical detection device better simultaneously and shoots and detect the chromatography membrane strip.
Furthermore, a pair of air suction fans and a pair of air blowing fans are respectively arranged on two sides of the thermal cycling device, and the air suction fans and the air blowing fans are symmetrically arranged with each other, so that heat in the nucleic acid extraction amplification detection device can be conveniently removed.
Furthermore, the front door and the operation display screen are arranged on one side of the working box body, so that the working box body is convenient to clean through the front door, the nucleic acid extraction amplification detection device is automatically extracted, amplified and detected through the operation display screen, and convenience is greatly improved.
Furthermore, the plurality of holding tanks can be used for holding reagents required by various reactions of a sample experiment or used as containers for reaction or detection and the like.
The invention has the beneficial effects that:
(1) the invention relates to a full-automatic nucleic acid extraction amplification detector, which comprises a working box body and an operation display screen, wherein a core device is arranged in the working box body and is electrically connected with the operation display screen, the core device comprises a nucleic acid extraction amplification detection device, a thermal cycle device, an adjustable light supplement lamp device and an optical detection device, the optical detection device is arranged in the core device and is positioned above the nucleic acid extraction amplification detection device, the nucleic acid extraction amplification detection device extracts, amplifies and detects nucleic acid by pressing a start button on the operation display screen, the amplification and detection of at least one extracted target template can be realized, the adjustable light supplement lamp device can automatically light up after the detection of the nucleic acid extraction amplification detection device is finished, so that the optical detection device above the nucleic acid extraction amplification detection device can be used for photographing and detecting chromatographic membrane strips, the detection condition of the sample is displayed by operating the display screen, the whole process can be automatically carried out, manual interference is not needed, the automation degree is high, errors and cross contamination caused by manual detection are avoided, the efficiency is greatly improved, the structure is not complicated, the efficiency, the accuracy and the operability of nucleic acid detection are effectively improved, human resources can be effectively saved, and the cost can be effectively reduced by detecting through an optical detection device without using a fluorescent probe;
(2) the nucleic acid extraction amplification detection device comprises a shell and a chassis, wherein the chassis is positioned at the lower end of the shell and is connected with the shell to form a sealed space, a lifting rotating assembly is installed and connected at the center of the chassis, one side of the lifting rotating assembly is connected with a suction assembly, the lifting assembly drives the suction assembly to lift and rotate so as to realize that the suction assembly performs liquid suction and liquid discharge between a plurality of accommodating grooves, the lifting rotating assembly is arranged to control the suction assembly to switch among the plurality of accommodating grooves, so that the cross contamination can be effectively avoided, the liquid suction or liquid discharge in different accommodating grooves can be realized, and the operation is simple and convenient;
(3) the invention installs the nucleic acid detection and calibration device above the nucleic acid extraction amplification detection device, the nucleic acid detection and calibration device comprises a second stepping motor, a second screw rod pair positioned below the second stepping motor and an elastic lifting platform, the second screw rod pair is arranged above the elastic lifting platform, one end of the second screw rod pair is connected with the second stepping motor, the other end of the second screw rod pair is connected with the elastic lifting platform, the second screw pair is driven by the second stepping motor to move downwards so as to press the elastic lifting platform on the nucleic acid extraction amplification detection device and enable the nucleic acid extraction amplification detection device and the thermal cycle device to be tightly attached together, therefore, the thermal cycling device and the nucleic acid detection and calibration device achieve better heat conduction effect, and provide proper temperature for the nucleic acid detection and calibration device to perform sample nucleic acid extraction and amplification reaction;
(4) the invention also arranges a compression spring on the elastic lifting platform, which prevents the elastic lifting platform from overshooting when pressing on the nucleic acid extraction amplification detection device;
(5) the adjustable light supplementing lamp device comprises a light fixing frame, a lamp strip mounting seat, a lamp strip, a fixing shaft and a rotating shaft sleeve, wherein the lamp strip mounting seat is mounted on the light fixing frame by one end of the fixing shaft, the other end of the fixing shaft is connected with the rotating shaft sleeve, and the lamp strip on the lamp strip mounting seat is driven to rotate by the rotating shaft sleeve, so that the light is conveniently adjusted, the convenience and the flexibility are improved, the sample experiment condition on the nucleic acid extraction amplification detection device can be conveniently detected, and meanwhile, the optical detection device is better assisted to photograph and detect the chromatographic membrane strip;
(6) the invention is characterized in that the two sides of the thermal circulation device are respectively provided with a pair of air suction fans and a pair of air blowing fans, and the air suction fans and the air blowing fans are symmetrically arranged with each other, so that the heat in the nucleic acid extraction amplification detection device can be conveniently removed, the service life of the full-automatic nucleic acid extraction amplification detection device can be protected and delayed, and the experimental safety can be improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a fully automatic nucleic acid amplification detector according to the present invention;
FIG. 2 is a schematic view of another embodiment of the automatic nucleic acid amplification detector of the present invention;
FIG. 3 is an exploded view of the overall structure of a fully automatic nucleic acid amplification detector according to the present invention;
FIG. 4 is a structural diagram of a movement device of the automatic nucleic acid amplification detector of the present invention;
FIG. 5 is a diagram showing the installation relationship between the nucleic acid amplification detecting device and the thermal cycling device of the fully automatic nucleic acid amplification detecting apparatus according to the present invention;
FIG. 6 is a schematic diagram of the external structure of a nucleic acid amplification detecting apparatus of the fully automatic nucleic acid amplification detecting apparatus according to the present invention;
FIG. 7 is a diagram showing the installation relationship between the nucleic acid amplification detecting device and the thermal cycling device, the adjustable fill-in light device, and the optical detecting device of the automatic nucleic acid amplification detecting apparatus according to the present invention; a
FIG. 8 is a schematic diagram of the internal structure of the nucleic acid amplification detection apparatus of the fully automatic nucleic acid amplification detection apparatus according to the present invention;
FIG. 9 is a schematic structural diagram of an air pressure flux driving device of the automatic nucleic acid amplification detector of the present invention;
FIG. 10 is a schematic diagram of a nucleic acid detecting and calibrating apparatus of the fully automatic nucleic acid extracting and amplifying detector according to the present invention;
FIG. 11 is a schematic structural diagram of a rotation driving device of the fully automatic nucleic acid amplification detection apparatus according to the present invention;
FIG. 12 is another schematic view of the rotation driving device of the automatic nucleic acid amplification detection apparatus according to the present invention
FIG. 13 is a schematic structural diagram of an adjustable fill-in light device of the full-automatic nucleic acid amplification detector of the present invention;
FIG. 14 is a schematic diagram of a fully automatic nucleic acid amplification detection apparatus according to a first embodiment of the present invention;
in the figure, the working chamber 1, the movement device 2, the nucleic acid extraction amplification detection device 21, the housing 211, the sample application well 2111, the sample application cover 2112, the magnetic sensor 2113, the magnetic bead adsorption assembly 2114, the bottom plate 212, the housing groove 2121, the sample groove 21211, the binding groove 21212, the magnetic bead groove 21213, the amplification groove 21214, the detection groove 21215, the waste liquid groove 21216, the K1 groove 21217, the K2 groove 21218, the buffer groove 21219, the K5 groove 212110, the K6 groove 212111, the K17 groove 212112, the K9 groove 212113, the chromatographic membrane strip 2122, the color development position 2123, the lifting and rotating assembly 2124, the screw rod 21241, the connecting arm 21242, the second pinion 21243, the second bull gear 21244, the magnet 2125, the suction assembly 2127, the suction barrel 21271, the suction cavity 21272, the hose 21273, the piston assembly 21273, the large piston cavity 21274, the atmospheric pressure cavity 21275, the small pressure cavity 5876, the small pressure cavity 21277, the air pressure chamber 2128, the stepping motor 2128, the fourth stepping motor 21282, a fourth guide rail 21283, a fifth stepping motor 21284, a fifth screw rod pair 21285, a fifth guide rail 21286, a push rod assembly 21282, a small push rod 212821, a large push rod 212822, a power device 22, a first stepping motor 221, a first screw rod pair 222, a first guide rail 223, a thermal circulation device 23, an optical coupling sensor 231, an adjustable light supplement lamp device 24, a light fixing frame 241, a light bar mounting seat 242, a light bar 243, a fixing shaft 244, a rotating shaft sleeve 245, an optical detection device 25, a nucleic acid detection calibration device 26, a second stepping motor 261, a second screw rod pair 262, a second guide rail 263, a second guide rail connecting frame 264, an elastic lifting platform 265, a rotation driving device 27, a motor driving assembly 271, a third stepping motor 2711, a third screw rod pair 2712, a sixth stepping motor 2713, a driving wheel 2714, a driving shaft 2715, a driving belt 2716, a first pinion 272, a first gearwheel 273, a control board 28, a driving power supply 29, a driving power supply, The front door 3, the handle 31, the operation display screen 4, the switch socket 5, the data serial port 51, the first air port 6, the second air port 7, the air suction fan 8 and the air blowing fan 9.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
Referring to fig. 1-3, the invention discloses a full-automatic nucleic acid extraction amplification detector, which comprises a work box body 1, a front door 3 and an operation display screen 4, wherein the front door 3 and the operation display screen 4 are arranged in front of the work box body 1, the operation display screen 4 is arranged above the front door 3, the front door 3 is provided with a handle 31, the front door 3 is opened and closed through the handle 31, a switch socket 5 and a data serial port 51 are arranged behind the work box body 1, the switch socket 5 is arranged on one side of the data serial port 51, and the work box body 1 is connected with commercial power through the switch socket 5;
preferably, a first air opening 6 and a second air opening 7 are respectively arranged on the left side and the right side of the working box body 1, and the first air opening 6 and the second air opening 7 are symmetrically arranged;
preferably, a top cover is arranged above the work box body 1 and used for covering the work box body 1, a core device 2 is arranged inside the work box body 1, and the core device 2 is electrically connected with the operation display screen 4.
Referring to fig. 4-5, preferably, the movement device 2 includes a nucleic acid extraction and amplification detection device 21, a thermal cycling device 23, an adjustable supplementary lighting device 24, and an optical detection device 25, the thermal cycling device 23 is installed inside the movement device 2, the nucleic acid extraction and amplification detection device 21 is installed above the thermal cycling device 23, the thermal cycling device 23 provides a suitable temperature for the movement of the nucleic acid extraction and amplification detection device 21 and the nucleic acid extraction and amplification of a sample, the adjustable supplementary lighting device 24 is installed above the optical detection device 25 through two connectors, and the optical detection device 25 is installed inside the movement device 2 and above the nucleic acid extraction and amplification detection device 21;
preferably, the movement device 2 comprises a nucleic acid detection calibration device 26 and a rotation driving device 27, the nucleic acid detection calibration device 26 is installed above the nucleic acid extraction and amplification detection device 21 so as to realize the close fit of the nucleic acid extraction and amplification detection device 21 and the thermal cycling device 23, and the rotation driving device 27 is located above the nucleic acid detection calibration device 26 and is movably connected with the nucleic acid detection calibration device.
Preferably, the movement device 2 comprises a control board 28 and a driving power supply 29, wherein the control board 28 is used for controlling the operation of the operation display screen 4, the driving power supply 29 is used for driving the operation display screen 4 to work, and the control board 28 is used for operating the operation display screen 4 to work.
Referring to fig. 5 to 8, the nucleic acid amplification detecting device 21 includes a sample application hole 2111 and a plurality of holding tanks 2121 for holding a sample and a solution for extracting nucleic acid and the like from the sample, and the nucleic acid amplification detecting device 21 further includes a chromatographic strip 2122, wherein the chromatographic strip 2122 is located at the periphery of the plurality of holding tanks 2121;
a power device 22 is arranged on one side of the nucleic acid extraction and amplification detection device 21, and one end of the power device 22 and one end of the nucleic acid extraction and amplification detection device 21 are connected, so that the power device 22 drives the nucleic acid extraction and amplification detection device 21 to move to a working position on a thermal cycling device 23;
an optical coupling sensor 231 for sensing the movement position of the nucleic acid extraction amplification detection device 21 is arranged above the thermal cycling device 23;
preferably, the power device 22 includes a first stepping motor 221, a first screw pair 222, and a first guide rail 223, the first screw pair 222 is located at one end of the first stepping motor 221 and one end of the first screw pair is connected to the first stepping motor 221, the other end of the first screw pair 222 is connected to the nucleic acid extraction amplification detection device 21, the first guide rail 223 is installed at one side of the first screw pair 222, and the first stepping motor 221 drives the first screw pair 222 to move the nucleic acid extraction amplification detection device 21 to the optical coupling sensor 231 above the thermal cycling device 23 along the direction of the first guide rail 223 to reset or move to a working position.
Specifically, the front door 3 is opened by the handle 31, the sample is injected into the plurality of accommodating grooves 2121 through the sample adding hole 2111 to extract, amplify and detect the nucleic acid of the sample, the chromatographic membrane strip 2122 is used for chromatographically detecting the sample and the nucleic acid in the plurality of accommodating grooves 2121, the operation display screen 4 is provided with a start button, the start button is pressed, the power device 22 moves the nucleic acid extraction and amplification detection device 21 to the position of the optical coupling sensor 231 to reset and detect the nucleic acid extraction and amplification in the sample, after the nucleic acid extraction and amplification detection device 21 detects the nucleic acid in the sample, the adjustable fill light lamp device 24 is automatically turned on so as to assist the optical detection device 25 above the nucleic acid extraction and amplification detection device 21 to photograph and detect the chromatographic membrane strip 2122, the detection condition of the sample is displayed by operating the display screen 4, and the degree of automation is high, the method avoids errors and cross contamination caused by manual detection, greatly improves the efficiency, is not complicated in structure, and can effectively reduce the cost by detecting through the optical detection device 25 without using a fluorescent probe.
Specifically, the nucleic acid extracting, amplifying and detecting device 21 includes a housing 211 and a base 212, the base 212 is located at the lower end of the housing 211 and is connected to the housing 211 to form a sealed space, the accommodating grooves 2121 include a sample groove 21211, a binding groove 21212, a magnetic bead groove 21213, an amplifying groove 21214, a detecting groove 21215, and a waste liquid groove 21216, the binding groove 21212 and the magnetic bead groove 21213 are used for accommodating a reactant and magnetic beads, a sample accommodated in the sample groove 21211 is lysed by the thermal cycling device 23, nucleic acid is extracted by the magnetic beads in the binding groove 21212 and the magnetic bead groove 21213, and the accommodating grooves 2121 are distributed on the circumferential curved surface of the base 212;
preferably, the four chromatographic strips 2122 are provided, and the four chromatographic strips 2122 are close to the detection groove 21215 for absorbing the liquid in the detection groove 21215, and when the solution for sample reaction is added into the holding groove 2121 for detection by the chromatographic strips 2122, a chromatographic phenomenon occurs, and a product component related to sample amplification is hybridized with a component at a corresponding portion of the chromatographic strips 2122, thereby forming colored strips;
preferably, a color display position 2123 is further disposed on the chassis 212 for displaying the color stripes. The color development position 2123 can be detected by the optical detection device 25 outside the housing 211;
preferably, the upper end of the sample adding hole 2111 is provided with a sample adding hole cover 2112, and the sample adding hole 2111 is covered by the sample adding hole cover 2112 so as to play a role in sealing;
preferably, a lifting and lowering rotary assembly 2124 is installed and connected to the center of the base plate 212, a suction assembly 2127 is disposed at one side of the lifting and lowering rotary assembly 2124, and one side of the lifting and lowering rotary assembly 2124 is connected to the suction assembly 2127 to drive the suction assembly 2127 to lift, lower and rotate so as to suck and discharge liquid into and from the plurality of accommodating grooves 2121 by the suction assembly 2127, and cross contamination can be effectively avoided by arranging the lifting and lowering rotary assembly 2124 to control the suction assembly 2127 to switch between the plurality of accommodating grooves 2121.
Preferably, the suction assembly 2127 comprises a suction barrel 21271, a suction chamber 21272, a hose 21273, and a piston assembly 21273, wherein the suction barrel 21271 is used for sucking and discharging the sample and the sample nucleic acid in the plurality of receiving grooves 2121, the suction barrel 21271 is located below and connected to the suction chamber 21272, the hose 21273 is disposed between the suction chamber 21272 and the piston assembly 21273, the piston assembly 21273 is located at one side of the suction chamber 21272, and the suction chamber 21272 is connected to the piston assembly 21273 via a hose 21273;
specifically, the piston assembly 21273 includes a large piston cavity 21274, an atmospheric pressure cavity 21275, a large piston cavity 21274 located above the atmospheric pressure cavity 21275 and connected thereto to form a large piston pneumatic pressure cavity system, the piston assembly 21273 further includes a small piston cavity 21276, a small pneumatic pressure cavity 21277, the small piston cavity 21276 located above the small pneumatic pressure cavity 21277 and connected thereto to form a small piston pneumatic pressure cavity system, a connecting sleeve 21278 is disposed between the large piston pneumatic pressure cavity system and the small piston pneumatic pressure cavity system, the large piston pneumatic pressure cavity system and the small piston pneumatic pressure cavity system are connected via the connecting sleeve 21278, so that the large piston cavity 21274, the atmospheric pressure cavity 21275, the small piston cavity 21276, the small pneumatic pressure cavity 21277, and the connecting sleeve 21278 form a piston assembly 21273, and a pneumatic flux pushing device 2128 is disposed above the piston assembly 21273;
preferably, the lifting and rotating assembly 2124 comprises a screw rod 21241, a connecting arm 21242, a second small gear 21243 and a second large gear 21244, one end of the connecting arm 21242 is sleeved on the screw rod 21241, the other end of the connecting arm is connected with a suction barrel 21271 through a suction cavity 21272, the second large gear 21244 is connected with the upper end of the screw rod 21241, and the second small gear 21243 is mounted above the second large gear 21244 so as to lift the screw rod 21241 and further to rotate the suction barrel 21271.
Preferably, the outer surface of the housing 211 is provided with a magnetic sensor 2113, one end of the connecting arm 21242 of the nucleic acid extraction and amplification detection device 21 is provided with a magnet 2125 in an outward extending manner, and the rotation driving device 27 drives the elevation rotation assembly 2124 to rotate and elevate so as to drive the magnet 2125 on the connecting arm 21242 to rotate and elevate so as to make the magnet 2125 and the magnetic sensor in the same horizontal and vertical direction, thereby resetting the nucleic acid extraction and amplification detection device 21;
preferably, a magnetic bead adsorption component 2114 is arranged on one side of the housing 211, the magnetic bead adsorption component 2114 is mounted on one side of the housing 211 through a mounting rack, and the magnetic bead adsorption component 2114 controls the magnet 2125 of the connecting arm 21242 of the nucleic acid extraction and amplification detection device 21 to the working position where the operation display screen 4 is arranged, so as to control the magnetic beads in the binding groove 21212 and the magnetic bead groove 21213 on the bottom plate 212 to perform nucleic acid adsorption on the sample, thereby realizing the extraction of the nucleic acid in the sample.
Preferably, the magnetic bead can be a superparamagnetic microsphere, and the superparamagnetic microsphere has the characteristics of superparamagnetism, quick magnetic responsiveness, rich carboxyl functional groups, very high specific surface area, monodispersity, submicron-scale particle size, good long-term stability, good batch repeatability and the like, can covalently couple biological ligands such as polypeptide, protein, oligonucleotide and the like to the surface of the microsphere under the action of a special chemical reagent (such as EDC), and is an important carrier tool in medical and molecular biological research.
Preferably, a pair of air suction fans 8 and a pair of air blowing fans 9 are respectively arranged on two sides of the thermal cycling device 23, the air suction fans 8 and the air blowing fans 9 are symmetrically arranged with each other to facilitate the removal of heat in the nucleic acid extracting and amplifying detection device 21, and the heat removed by the air suction fans 8 and the air blowing fans 9 is discharged from the first air port 6 and the second air port 7;
preferably, the front door 3 and the operation display screen 4 are arranged on one side of the work box body 1, so that the work box body 1 can be cleaned through the front door 3 conveniently, and the nucleic acid extraction amplification detection device 21 can be automatically extracted, amplified and detected through the operation display screen 4, so that the convenience is greatly improved.
Referring to fig. 9, the pneumatic flux driving unit 2128 includes a stepping driving unit 21281 and a push rod unit 21282, and the stepping driving unit 21281 drives the push rod unit 21282 to move the piston unit 21273 back and forth to suck air into the suction chamber 21272 and to suck the solution from the plurality of receiving grooves 2121 or discharge the solution into the plurality of receiving grooves 2121 by the suction barrel 21271.
Preferably, the push rod assembly 21282 comprises a small push rod 212821 and a large push rod 212822, and the small push rod 212821 and the large push rod 212822 are oppositely arranged.
Preferably, the stepping driving assembly 21281 includes a fourth stepping motor 21281 and a fourth lead screw pair 21282 located below the fourth stepping motor 21281, one side of the fourth lead screw pair 21282 is provided with a fourth guide rail 21283, one end of the fourth lead screw pair 21282 is connected to the fourth stepping motor 21281, the other end of the fourth lead screw pair 21282 is connected to the small push rod 212821, the fourth lead screw pair 21282 is driven by the fourth stepping motor 21281 to move along the fourth guide rail 21283 so as to drive the small push rod 212821 to push the small piston cavity 21276, and due to the small spring arranged in the small piston cavity 21276, the small push rod 212821 pushes the small piston cavity 21276 to reciprocate in the small pneumatic cavity 21277, so that the liquid suction gun tube 21271 sucks liquid in the accommodating grooves 2121 through the piston assembly 21273.
Preferably, the stepping driving assembly 21281 further includes a fifth stepping motor 21284 and a fifth screw pair 21285 located below the fifth stepping motor 21284, one side of the fifth screw pair 21285 is provided with a fifth guide rail 21286, one end of the fifth screw pair 21285 is connected to the fifth stepping motor 21284, the other end of the fifth screw pair 21285 is connected to a large push rod 212822, the fifth screw pair 21285 is driven by the fifth stepping motor 21284 to move along the direction of the fifth guide rail 21286 so as to drive the large push rod 212822 to push the large piston cavity 21274, and a large spring is disposed in the large piston cavity 21274, so that the large push rod 212822 pushes the large piston cavity 21274 to reciprocate in the atmospheric pressure cavity 21275, and the liquid suction gun barrel 21271 discharges liquid in the accommodating grooves 2121 through the piston assembly 21273.
Referring to fig. 10, the nucleic acid detecting and calibrating device 26 includes a second stepping motor 261, a second screw pair 262 located below the second stepping motor 261, an elastic lifting platform 265, a second guide rail 263 and a guide rail connecting frame 264, the second screw pair 262 is installed above the elastic lifting platform 265, one end of the second screw pair 262 is connected with the second stepping motor 261, the other end of the second screw pair 262 is connected with the elastic lifting platform 265, the second guide rail 263 is installed on one side of the second screw pair 262 through the guide rail connecting frame 264, the second stepping motor 261 drives the second screw pair 262 to move downwards along the direction of the second guide rail 263 so as to press the elastic lifting platform 265 on the nucleic acid extracting and amplifying detecting device 21, so that the nucleic acid extracting and amplifying detecting device 21 is closely attached to the thermal cycling device 23;
preferably, the elastic lifting platform 265 is provided with a compression spring, and the compression spring plays a role in preventing overshoot when the elastic lifting platform 265 presses the nucleic acid extraction amplification detection device 21, and plays a role in protecting the nucleic acid extraction amplification detection device 21.
Referring to fig. 11-12, the rotary driving device 27 includes a motor driving component 271, a first small gear 272, and a first large gear 273, the first small gear 272 is installed below the motor driving component 271, the first large gear 273 is installed below the first small gear 272 so as to form the rotary driving device 27 for driving the lifting rotary component 2124 to rotate and lift, the motor driving component 271 drives the first small gear 272 and the first large gear 273 to cooperate with each other so as to drive the first small gear 272 and the first large gear 273 to rotate automatically;
preferably, the motor driving assembly 271 comprises a third stepping motor 2711, a third screw pair, a sixth stepping motor 2713, a driving wheel 2714 and a driving shaft 2715, the third screw pair is located below the third stepping motor 2711 and at one end of the driving wheel 2714, one end of the third screw is connected to the third stepping motor 2711, the other end thereof is located on one end of a driving wheel 2714, said driving shaft 2715 is located below and connected to a sixth stepping motor 2713, the drive shaft 2715 is also located above the first large gear 273 and the first small gear 272, the third stepping motor 2711 drives the third screw pair to rotate so as to drive the driving wheel 2714 to rotate, the driving wheel 2714 and the third stepping motor 2711 are connected by a transmission belt 2716, therefore, the rotation of the driving wheel 2714 also drives the rotation of the sixth stepping motor 2713 to drive the driving shaft 2715, and the rotation of the driving shaft 2715 drives the matching and rotation of the first large gear 273 and the first small gear 272.
Referring to fig. 13, the adjustable light supplement lamp device 24 includes a light fixing frame 241, a light bar mounting seat 242, a light bar 243, a fixing shaft 244 and a rotating shaft sleeve 245, the light bar mounting seat 242, the light bar 243, the fixing shaft 244 and the rotating shaft sleeve 245 are all located above the light fixing frame 241, the light bar 243 is detachably mounted above the light bar mounting seat 242, the fixing shaft 244 is located at one end of the light bar mounting seat 242, the rotating shaft sleeve 245 is located at one side of the fixing shaft 244, the light bar mounting seat 242 is mounted on the light fixing frame 241 at one end of the fixing shaft 244, the other end is connected with the rotating shaft sleeve 245, the light bar 243 on the light bar mounting seat 242 is driven to rotate by the rotating shaft sleeve 245, so as to facilitate adjustment of light, improve convenience and flexibility, and facilitate detection of sample experiment conditions on the nucleic acid extraction and amplification, and also serves to better assist the optical detection device 25 in photographing and detecting the chromatographic strip 2122.
Preferably, the plurality of holding grooves 2121 can hold reagents required for various reactions of a sample experiment or serve as containers for reaction or detection, and the like.
The first embodiment is as follows: referring to fig. 14, fig. 13 is a schematic cross-sectional view of a base plate 212 according to a first embodiment of the present invention, which relates to the steps of the full-automatic PCR extraction, amplification, and detection experiment:
wherein, the experimental process of the equipment is as follows: the sample tank 21211 is raised to 70 ℃ by the thermal cycler 23 of the present invention;
1) preparing 100ul of sample, opening the front door 3 of the work box 1, opening the sample adding cover 2112, adding 100ul of sample to the sample groove 21211 through the sample adding hole 2111, and mixing uniformly;
2) selecting on the operation display screen 4 according to the detection items needed by the sample, pressing a start button on the operation display screen 4, resetting the nucleic acid extraction and amplification detection device 21 through the power device 22, and starting the operation of the full-automatic nucleic acid extraction and amplification detection device;
3) 50ul of PK solution was pipetted from a K1 well 21217 containing 80ul of PK solution through a pipette barrel 21271 and added to a sample well 21211;
4) draw 40u from K2 tank 21218, which contained 80ul of a tank of carrier solution, through pipette barrel 21271 into sample tank 21211;
5) heating the sample tank 21211 to 70 ℃ by the thermal cycling device 23, incubating for 10 minutes, passing through the pipette barrel 21271 per minute with a mixing action, wherein the mixing action is performed for 3 times, and the same applies below);
6) move the entire solution in sample well 21211 to buffer well 21219 via pipette barrel 21271 and stop heating;
7) mix the 200ul solution in the magnetic bead tank 21213 and then move the whole solution to the sample tank 21211 through the pipette barrel 21271;
8) controlling the magnet 2125 of the connecting arm 21242 of the nucleic acid extraction and amplification detection device 21 to a working position (referred to as an "upper magnet 2125" for short) arranged on the operation display screen 4 through the magnetic bead adsorption component 2114, so as to realize the adsorption of magnetic beads, after the magnetic beads are completely adsorbed, removing supernatant liquid of the magnetic bead to a K1 groove 21217 filled with PK solution, and adsorbing the magnetic beads in the magnetic bead groove 21213 for 3 minutes;
9) the magnetic bead adsorption component 2114 controls the magnet 2125 of the connecting arm 21242 of the nucleic acid extraction and amplification detection device 21 to be away from the working position (referred to as "demagnetizing iron 2125" for short) where the operation display screen 4 is arranged, so as to transfer 150ul of liquid from the combining groove 21212 and sufficiently mix the liquid with the magnetic beads;
10) after the magnetic beads are completely adsorbed, the upper magnet 2125 removes the supernatant to a K1 groove 21217 with PK solution, and the magnetic beads in the magnetic bead groove 21213 are adsorbed for 3 minutes; .
11) The solution in the buffer tank 21219 is completely transferred to the sample tank 21211 through the pipette barrel 21271 and mixed;
12) transfer 300ul of fluid from the binding well 21212 through pipette barrel 21271, mix well with magnetic beads, incubate for 3 minutes with mixing (mix 2 times every 30 seconds);
13) a magnet 2125, after the magnetic beads are completely adsorbed, removing the supernatant to a K2 tank 21218 containing a carrier solution;
14) demagnetizing iron 2125, removing 500ul of the solution from K5 tank 212110 containing 500ul of buffer solution, adding into sample tank 21211, and mixing with liquid absorbing gun barrel 21271 for several times;
15) an upper magnet 2125, after the magnetic beads are completely adsorbed, removing the supernatant to a K2 tank 21218 of the carrier solution;
16) demagnetizing iron 2125, transferring 500ul of the solution from a K6 tank 212111 containing 500ul of the washing buffer solution, adding the solution into a sample tank 21211, and uniformly mixing for multiple times;
17) a magnet 2125 is arranged, and after the magnetic beads are completely adsorbed, the supernatant is removed to a K2 tank 21218 containing a carrier solution;
18) the sample tank 21211 is heated to 70 ℃ and dried for 3 minutes;
19) cleaning the gun head, transferring 50ul of solution from a K17 tank 212111 containing 500ul of distilled water through a liquid absorbing gun tube 21271, adding the solution into a sample tank 21211 through the liquid absorbing gun tube 21271, uniformly mixing for two minutes, and standing for 3 minutes;
20) an upper magnet 2125, after the magnetic beads were completely adsorbed, 5ul of DNA was transferred from the sample well 21211 to the K1 well 21217
21) 40ul of solution removed from the K9 tank 212113 containing 150ul of mineral oil was added to the K1 tank 21217, where the PCR extraction process was completed, and the PCR temperature control process was entered
22) PCR reaction: a. raising the normal temperature to 95 ℃ by a thermal cycling device 23, and incubating for 2 minutes;
b, circulating for 35 times at the temperature of 95 ℃/5S-60 ℃/10S;
23) after the reaction, 20ul of the solution was removed from the K1 tank 21217 and added to the K2 tank 21218;
24) the solution in the K2 tank 21218 was incubated at room temperature for 15mins and detected by the chromatographic strip 2122.
The above-mentioned embodiments only express one embodiment of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The utility model provides a full-automatic nucleic acid draws amplificate detector, is including the work box, locate the qianmen and the operation display screen in work box the place ahead, and shown operation display screen is located the top of qianmen, the inside of work box is provided with the core device, core device and operation display screen electricity are connected, its characterized in that: the movement device comprises a nucleic acid extraction and amplification detection device for extracting, amplifying and detecting nucleic acid of a sample, a thermal circulation device for providing proper temperature for the movement of the nucleic acid extraction and amplification detection device and the extraction and amplification of the nucleic acid of the sample, an adjustable light supplement lamp device and an optical detection device, wherein the thermal circulation device is arranged in the movement device;
the nucleic acid extraction, amplification and detection device comprises a sample adding hole and a plurality of holding tanks, wherein the holding tanks can be used for holding samples and extracting solutions such as nucleic acid in the samples;
one side of the nucleic acid extraction amplification detection device is provided with a power device, and one end of the power device is connected with one end of the nucleic acid extraction amplification detection device, so that the power device drives the nucleic acid extraction amplification detection device to move to a working position on the thermal cycle device;
an optical coupling sensor for sensing the motion position of the nucleic acid extraction amplification detection device is arranged above the thermal cycling device, the front door is opened, the sample is injected into the plurality of accommodating tanks through the sample adding hole to carry out nucleic acid extraction, amplification and detection on the sample, the chromatographic membrane strip is used for chromatography and detection of samples and nucleic acids in a plurality of accommodating grooves, the operation display screen is provided with a start button, the start button is pressed down, the power device moves the nucleic acid extraction amplification detection device to the position of the optical coupling sensor to carry out resetting and nucleic acid extraction amplification detection work in the sample, after the nucleic acid extraction amplification detection device detects the nucleic acid in the sample, the adjustable light supplement lamp device automatically lights so as to assist the optical detection device above the nucleic acid extraction amplification detection device to photograph and detect the chromatographic membrane strip, and the detection condition of the sample is displayed by operating the display screen.
2. The fully automatic nucleic acid extraction amplification detector of claim 1, wherein: the core device comprises a nucleic acid detection and calibration device and a rotation driving device, wherein the nucleic acid detection and calibration device is arranged above the nucleic acid extraction and amplification detection device so as to realize the close attachment of the nucleic acid extraction and amplification detection device and the thermal cycling device, and the rotation driving device is positioned above the nucleic acid detection and calibration device and is movably connected with the nucleic acid detection and amplification detection device.
3. The fully automatic nucleic acid extraction amplification detector of claim 1, wherein: the power device comprises a first stepping motor and a first screw rod pair, the first screw rod pair is positioned at one end of the first stepping motor, one end of the first screw rod pair is connected with the first stepping motor, the other end of the first screw rod pair is connected with the nucleic acid extraction amplification detection device, and the first screw rod pair is driven by the first stepping motor to move the nucleic acid extraction amplification detection device to the position of the optical coupler sensor along the upper part of the thermal cycle device to reset or move to a working position.
4. The fully automatic nucleic acid extraction amplification detector of claim 1, wherein: the nucleic acid extraction amplification detection device comprises a shell and a chassis, wherein the chassis is positioned at the lower end of the shell and connected with the shell to form a sealed space, the holding tanks comprise a sample tank, a combination tank, a magnetic bead tank, an amplification tank, a detection tank and a waste liquid tank, the combination groove and the magnetic bead groove are used for placing reactants and magnetic beads, after the sample placed in the sample groove is cracked under the action of the thermal cycling device, the extraction of nucleic acid is carried out by combining the magnetic beads in the grooves and the magnetic bead grooves, a plurality of holding grooves are distributed on the circumferential curved surface of the chassis, the center of the chassis is provided with a lifting rotating component, one side of the lifting rotating component is provided with a suction component, thereby one side and the suction subassembly of lift rotating assembly are connected and drive suction subassembly and go up and down and rotate and then realize that suction subassembly carries out imbibition and flowing back between a plurality of holding tanks.
5. The apparatus according to claim 4, wherein the apparatus comprises: the suction assembly comprises a liquid suction barrel, a liquid suction cavity, a hose and a piston assembly, wherein the liquid suction barrel, the liquid suction cavity, the hose and the piston assembly are used for sucking liquid and discharging liquid from solutions such as samples and sample nucleic acids in a plurality of accommodating grooves, the liquid suction barrel is located below the liquid suction cavity and connected with the liquid suction barrel, the hose is arranged between the liquid suction cavity and the piston assembly, the piston assembly is located on one side of the liquid suction cavity, and the liquid suction cavity is connected with the piston assembly through the hose.
6. The apparatus according to claim 5, wherein the apparatus further comprises: the top of piston assembly is equipped with atmospheric pressure flux thrust unit, atmospheric pressure flux thrust unit is including step-by-step drive subassembly and push rod subassembly, the push rod subassembly is located step-by-step drive subassembly's below and is connected with it, thereby step-by-step drive subassembly drive push rod subassembly promotes piston assembly reciprocating motion and realizes absorbing the suction gas of imbibition cavity and then realizing that the imbibition barrel absorbs solution or to discharge solution in a plurality of holding tanks from a plurality of holding tank inside grooves.
7. The fully automatic nucleic acid extraction amplification detector of claim 2, wherein: the nucleic acid detection and calibration device comprises a second stepping motor, a second screw rod pair and an elastic lifting platform, wherein the second screw rod pair is positioned below the second stepping motor, the second screw rod pair is arranged above the elastic lifting platform, one end of the second screw rod pair is connected with the second stepping motor, the other end of the second screw rod pair is connected with the elastic lifting platform, and the second stepping motor drives the second screw rod pair to move downwards so as to press the elastic lifting platform on the nucleic acid extraction and amplification detection device and enable the nucleic acid extraction and amplification detection device to be tightly attached to the thermal circulation device.
8. The fully automatic nucleic acid extraction amplification detector of claim 2, wherein: the rotation driving device comprises a motor driving assembly, a first pinion and a first gearwheel, the first pinion is installed below the motor driving assembly, the first gearwheel is installed below the first pinion to form a rotation driving device which drives the lifting rotation assembly to rotate and lift, and the motor driving assembly drives the first pinion and the first gearwheel to be matched to drive the first pinion and the first gearwheel to automatically rotate.
9. The apparatus according to claim 4, wherein the apparatus comprises: the lifting rotating assembly comprises a screw rod, a connecting arm, a second pinion and a second gear wheel, one end of the connecting arm is sleeved on the screw rod, the other end of the connecting arm is connected with the liquid suction barrel through a liquid suction cavity, the second gear wheel is connected with the upper end of the screw rod, and the second pinion is mounted above the second gear wheel to lift the screw rod and further rotate the liquid suction barrel.
10. The apparatus according to claim 3, wherein the apparatus further comprises: the surface of shell is equipped with magnetic sensor, nucleic acid extraction amplification detection device's linking arm one end is outwards extended and is equipped with magnet, thereby rotate drive arrangement drive lift rotating assembly and rotate and go up and down to drive the magnet on the linking arm and rotate and go up and down and make magnet and magnetic sensor be in same level, vertical direction and then make nucleic acid extraction amplification detection device accomplish and reset.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010098156.3A CN111154641A (en) | 2020-02-17 | 2020-02-17 | Full-automatic nucleic acid extraction amplifications detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010098156.3A CN111154641A (en) | 2020-02-17 | 2020-02-17 | Full-automatic nucleic acid extraction amplifications detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111154641A true CN111154641A (en) | 2020-05-15 |
Family
ID=70565781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010098156.3A Pending CN111154641A (en) | 2020-02-17 | 2020-02-17 | Full-automatic nucleic acid extraction amplifications detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111154641A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111704993A (en) * | 2020-08-18 | 2020-09-25 | 中国农业大学 | Integrated nucleic acid POCT detection device and method |
| CN111996101A (en) * | 2020-08-07 | 2020-11-27 | 襄阳市远杰精密机械有限公司 | Full-automatic nucleic acid extraction instrument with reduced sample pollution probability |
| CN112625890A (en) * | 2020-12-23 | 2021-04-09 | 广州和实生物技术有限公司 | Nucleic acid POCT detection device and detection method thereof |
| CN113088445A (en) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | Full-automatic nucleic acid amplification detection analyzer |
| CN113214982A (en) * | 2021-06-09 | 2021-08-06 | 北京卓诚惠生生物科技股份有限公司 | PCR chamber |
| CN113373042A (en) * | 2021-06-09 | 2021-09-10 | 北京卓诚惠生生物科技股份有限公司 | Modularization nucleic acid extraction, amplification and detection all-in-one machine |
| CN114199631A (en) * | 2021-12-13 | 2022-03-18 | 杭州莱和生物技术有限公司 | Amplification method for immunochromatography monitoring signal |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104204229A (en) * | 2012-02-10 | 2014-12-10 | 株式会社百奥尼 | Apparatus and method for automatically analyzing biological samples |
| WO2017128806A1 (en) * | 2016-01-27 | 2017-08-03 | 广州万孚生物技术股份有限公司 | Automatic fluorescence quantitative immunoassay analyzer and detection method |
| CN107964505A (en) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | Multi-functional nucleic acid detection apparatus |
| US20190160468A1 (en) * | 2016-09-12 | 2019-05-30 | Delta Electronics Int'l (Singapore) Pte Ltd | Nucleic acid analysis apparatus |
| CN110564607A (en) * | 2019-09-11 | 2019-12-13 | 长春技特生物技术有限公司 | Full-automatic nucleic acid extraction amplification micro-fluidic chip dynamic quantitative detection integrated device |
-
2020
- 2020-02-17 CN CN202010098156.3A patent/CN111154641A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104204229A (en) * | 2012-02-10 | 2014-12-10 | 株式会社百奥尼 | Apparatus and method for automatically analyzing biological samples |
| WO2017128806A1 (en) * | 2016-01-27 | 2017-08-03 | 广州万孚生物技术股份有限公司 | Automatic fluorescence quantitative immunoassay analyzer and detection method |
| US20190160468A1 (en) * | 2016-09-12 | 2019-05-30 | Delta Electronics Int'l (Singapore) Pte Ltd | Nucleic acid analysis apparatus |
| CN107964505A (en) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | Multi-functional nucleic acid detection apparatus |
| CN110564607A (en) * | 2019-09-11 | 2019-12-13 | 长春技特生物技术有限公司 | Full-automatic nucleic acid extraction amplification micro-fluidic chip dynamic quantitative detection integrated device |
Non-Patent Citations (1)
| Title |
|---|
| 普冬等: "全自动核酸纯化系统在临床分子检测中的应用研究", 《中国热带医学》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111996101A (en) * | 2020-08-07 | 2020-11-27 | 襄阳市远杰精密机械有限公司 | Full-automatic nucleic acid extraction instrument with reduced sample pollution probability |
| CN111704993A (en) * | 2020-08-18 | 2020-09-25 | 中国农业大学 | Integrated nucleic acid POCT detection device and method |
| CN111704993B (en) * | 2020-08-18 | 2020-11-20 | 中国农业大学 | Integrated nucleic acid POCT detection device and method |
| CN112625890A (en) * | 2020-12-23 | 2021-04-09 | 广州和实生物技术有限公司 | Nucleic acid POCT detection device and detection method thereof |
| CN113088445A (en) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | Full-automatic nucleic acid amplification detection analyzer |
| CN113214982A (en) * | 2021-06-09 | 2021-08-06 | 北京卓诚惠生生物科技股份有限公司 | PCR chamber |
| CN113373042A (en) * | 2021-06-09 | 2021-09-10 | 北京卓诚惠生生物科技股份有限公司 | Modularization nucleic acid extraction, amplification and detection all-in-one machine |
| CN113214982B (en) * | 2021-06-09 | 2021-11-26 | 北京卓诚惠生生物科技股份有限公司 | PCR chamber |
| CN114199631A (en) * | 2021-12-13 | 2022-03-18 | 杭州莱和生物技术有限公司 | Amplification method for immunochromatography monitoring signal |
| CN114199631B (en) * | 2021-12-13 | 2022-08-12 | 杭州莱和生物技术有限公司 | Amplification method for immunochromatography monitoring signal |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111154641A (en) | Full-automatic nucleic acid extraction amplifications detector | |
| CN111057638B (en) | Sample processing device and method, and digital PCR system including the same | |
| US9335339B2 (en) | Specimen testing device and method thereof | |
| WO2022237181A1 (en) | Dna and rna nucleic acid co-extraction and detection system | |
| TWI391492B (en) | Automated system and method for processing genetic material | |
| WO2022134153A1 (en) | Nucleic acid poct testing apparatus and testing method thereof | |
| CN216998448U (en) | Full-automatic nucleic acid detector | |
| CN111778137B (en) | Three-degree-of-freedom library preparation card box and method | |
| CN114774264B (en) | Automatic air microorganism detection system | |
| CN110819623A (en) | Nucleic acid extraction instrument and extraction method | |
| CN107988045B (en) | Full-automatic nucleic acid purification system based on magnetic bead method | |
| CN210982475U (en) | Full-automatic water quality COD robot analyzer | |
| WO2020181825A1 (en) | Nucleic acid extraction apparatus | |
| CN208568812U (en) | A kind of Biochemical Analyzer | |
| CN210856132U (en) | Integrated nucleic acid detection device | |
| CN210923478U (en) | Real-time fluorescent quantitative PCR instrument for on-site rapid detection | |
| WO2021031227A1 (en) | Integrated nucleic acid detection device and application | |
| CN212864734U (en) | Automatic nucleic acid extraction instrument | |
| CN108315243B (en) | Automatic sample adding system | |
| CN211848004U (en) | Nucleic acid extraction instrument | |
| CN212270077U (en) | Nucleic acid extraction device | |
| CN114703050A (en) | Automatic preparation device and preparation method of RNA | |
| CN215162724U (en) | Nucleic acid extraction all-in-one machine | |
| CN218811752U (en) | Nucleic acid extraction, detection and analysis integrated instrument | |
| CN108275318B (en) | Convenient chip heat-sealing instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |