CN104450498A - Nucleic acid extractor - Google Patents
Nucleic acid extractor Download PDFInfo
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- CN104450498A CN104450498A CN201410775075.7A CN201410775075A CN104450498A CN 104450498 A CN104450498 A CN 104450498A CN 201410775075 A CN201410775075 A CN 201410775075A CN 104450498 A CN104450498 A CN 104450498A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention relates to the technical field of medical equipment and particularly relates to a nucleic acid extractor. The nucleic acid extractor comprises a shell, a rack, a nucleic acid extracting module, a sample bin, a reagent region, a multi-pipette module and a single-pipette module, wherein a table board is arranged on the rack; the nucleic acid extracting module, the sample bin and the reagent region are arranged on the table board; the multi-pipette module and the single-pipette module are positioned above the table board; an X arm is arranged on the rack; a multi-pipette Y arm and a single-pipette Y arm are arranged on the X arm; the multi-pipette Y arm and the single-pipette Y arm slide along the X arm; the multi-pipette module is arranged on the multi-pipette Y arm and slides along the multi-pipette Y arm; the single-pipette module is arranged on the single-pipette Y arm and slides along the single-pipette Y arm. The invention provides the nucleic acid extractor which is simple in structure, capable of preventing cross contamination and small in difference.
Description
Technical field
The present invention relates to technical field of medical equipment, specifically a kind of instrument for extracting nucleic acid.
Background technology
The biomacromolecule compound that nucleic acid is become by many nucleotide polymerization is one of the most base substance of life.Nucleic acid is extensively present in all animal and plant cellss, microbe, and the normal and protein bound of the nucleic acid in organism forms nucleoprotein.Different nucleic acid, its difference such as chemical constitution, nucleotidesequence.
Nanometer magnetic bead is the super-paramagnetism nano microballoon of a kind of surface with carboxyl, and Absorbable organic halogens is scattered in (as damping fluids such as phosphoric acid salt, Tris, carbonate, borates) in the biological buffer solutions of water and PH>6.5.Its rich surface is containing carboxyl, this carboxyl can with biological aglucon covalent attachment, because of it, there is superparamagnetism simultaneously, sharp separation can be realized under externally-applied magnetic field, therefore can be widely used in the fields such as biomarker, immunodiagnosis, protein separation, cellular segregation, immobilized enzyme, biosensor, be particularly useful for high throughput automated platform.
Almost in each laboratory, the separation and purification work relevant to biomolecules is all very important, and requisite.But manually purifying still suitable difficulty is carried out to sample, not only need to select suitable purification technique, and workload is also large especially, poor repeatability.Be difficult to meet extraction purification is carried out in current develop rapidly demand to high-throughput sample.Current inspection body takes artificial experimental implementation to coordinate the mode of semi-automatic extraction device mostly, and extraction element only has single magnetic bead locomotive function, and the pre-treatment of sample, reagent all has operator manually to complete with the work such as to be separated of extracting product.There is individual difference in manual operation, not only affects the accuracy of result, also limit the flux of detection when sample size is various.
Summary of the invention
The invention provides a kind of structure simple, prevent crossed contamination, instrument for extracting nucleic acid that otherness is little.
The present invention is achieved through the following technical solutions:
A kind of instrument for extracting nucleic acid, comprise shell, frame, nucleic acid extraction module, sample storehouse, reagent area, volley of rifle fire module and single rifle module, described frame is provided with table top, described nucleic acid extraction module, sample storehouse and reagent area are all located on table top, described volley of rifle fire module and single rifle module are positioned at above described table top, described frame is provided with X arm, described X arm is provided with volley of rifle fire Y arm and single rifle Y arm, described volley of rifle fire Y arm and single rifle Y arm slide along X arm, described volley of rifle fire module is located on described volley of rifle fire Y arm, and slide along volley of rifle fire Y arm, described single rifle module is located on described single rifle Y arm, and slide along single rifle Y arm.
Described nucleic acid extraction module comprises housing, base, comb, vibrating mechanism, heating arrangements and magnetic suck mechanism, described heating arrangements is located on the upper surface of described base, described comb is located on described vibrating mechanism, described vibrating mechanism is positioned at above described heating arrangements, described magnetic suck mechanism is between described heating arrangements and described vibrating mechanism, and move along described heating arrangements upper surface, described housing is located at above described base and to be positioned at described vibrating mechanism, heating arrangements and magnetic suck mechanism outside.
Described vibrating mechanism involving vibrations plate, the first motor, two excentric shafts and two briquettings, described comb is located on described oscillating plate, described first motor is located on described base, two excentric shafts are located at the two ends of described oscillating plate respectively, described excentric shaft is arranged with bearing, and compressed by briquetting, the upper end of described excentric shaft and the output terminal of the first motor are equipped with the first synchronous pulley, are connected by two excentric shafts by the first Timing Belt with the first motor.
Described heating arrangements comprises the first linear electric motors, hot-plate and heating piece, described heating piece is located at below described hot-plate, and compressed by pressing plate, described hot-plate upper surface is provided with thermal baffle, described hot-plate two ends are respectively provided with first guide rod downwards, the middle part of described hot-plate is to having a rhizoid bar, two first lower end of the guide rod are through described base, first linear electric motors are located on the lower surface of described base, described screw mandrel lower end is through described base, and be connected with described first linear electric motors, described hot-plate is provided with some bottoming holes, described thermal baffle is provided with some through holes with described bottoming hole corresponding section.
Described magnetic suck mechanism comprises magnet steel seat, magnet steel and the second motor, described magnet steel seat is provided with some magnet steel mounting grooves, described magnet steel is located in described magnet steel mounting groove, and compressed by magnet steel lid, by screw, described magnet steel lid is connected with described magnet steel seat, two tail rods be parallel to each other are provided with between the internal surface that two of described housing are corresponding, described magnet steel seat is located on two tail rods, described second motor is located on the internal surface of described housing, and be connected with described magnet steel seat by the second Timing Belt, described second Timing Belt is connected with described magnet steel seat by Timing Belt clamping piece.
Described volley of rifle fire module comprises volley of rifle fire backboard, the 3rd motor, volley of rifle fire head group part, hoisting appliance and slide mechanism, described volley of rifle fire backboard is fixed on described volley of rifle fire Y arm, the top of described volley of rifle fire backboard is located at by described 3rd motor, and be connected with described hoisting appliance, described volley of rifle fire head group part is slidably connected by slide mechanism and described volley of rifle fire backboard, and realizes sliding up and down by hoisting appliance.
Described volley of rifle fire head group part comprises some volley of rifle fire heads, the rifle headstock, fixed block and volley of rifle fire buffer gear, described volley of rifle fire head is vertical on the described rifle headstock, the middle part of described volley of rifle fire head is arranged with sliding sleeve and the first compression spring, described first compression spring is positioned at above described sliding sleeve, the upper end of described volley of rifle fire head is arranged with joint, and upper end is arranged with non-conductive suction nozzle; The described rifle headstock is connected with described fixed block by rifle head fixture block, described volley of rifle fire buffer gear comprises the second guide rod and the second compression spring, described second guide rod upper end is connected with described fixed block, lower end is inserted in described rifle head fixture block, the lower end of described second guide rod is arranged with the first linear bearings, described first linear bearings is between described second guide rod and described rifle head fixture block, and described second compression spring is sheathed on described second guide rod, and is positioned at above described first linear bearings.
Described hoisting appliance comprises the first screw rod and nut, described 3rd motor output end is provided with motion-work wheel, described first screw rod upper end is arranged with follow-up pulley and two head bearings, described follow-up pulley is connected with described motion-work wheel by the 3rd Timing Belt, described first screw rod lower end is arranged with a lower bearing, described head bearing is fixed by head bearing frame, and with the first set nut locking, described lower bearing is fixed by lower bearing bracket, described nut sleeve is located at described first screw rod middle and upper part, and it is inner to be positioned at described fixed block, is fixed by nut gland.
Described slide mechanism comprises the first guide rail and slide block, and described first guide rail is vertically located on described volley of rifle fire backboard, and described slide block is located on described fixed block, and described slide block is stuck on described first guide rail, and slides up and down along the first guide rail.
Described single rifle module comprises single rifle backboard, single rifle transfer block, single rifle head, piston, cylinder body, the second linear electric motors, first circuit board and filling mechanism, described liquid feeding structure comprises the 4th motor, liquid feeding pin and the 3rd linear electric motors, described 4th motor drives described single rifle transfer block to move up and down by the 4th Timing Belt, and described 3rd linear electric motors drive liquid feeding pin to move up and down, described single rifle backboard is fixed on described single rifle Y arm, the output terminal of described second linear electric motors is connected by the upper end of piston transfer block with described piston, described piston is located at described cylinder interior, described single rifle head upper end is connected with described cylinder body lower end by insulation joint pin, and be connected with described cylinder interior, described cylinder body is provided with pneumatic fittings, described pneumatic fittings is connected with described cylinder body, described first circuit board is provided with the baroceptor be connected with described pneumatic fittings, described single rifle head is provided with the conducting block be connected with first circuit board, described second linear electric motors are electrically connected with first circuit board, described single rifle head lower end is provided with conduction suction nozzle.
Described single rifle module also comprises two groups of list rifle buffer gears, described single rifle buffer gear comprises the 3rd guide rod, the 3rd compression spring and the second linear bearings, the upper end of described 3rd guide rod is connected with described single rifle transfer block, lower end is connected with described cylinder body, and fixed by the second set nut, described 3rd compression spring and the second linear bearings are all sheathed on described 3rd guide rod, described 3rd compression spring is between described second linear bearings and described single rifle transfer block, and described second linear bearings outer cover is provided with linear axis bearing sleeve.
Vertical direction is provided with two the 4th guide rods be parallel to each other between described piston transfer block and described single rifle transfer block, described 4th guide rod upper end is fixedly connected with described single rifle transfer block, and lower end and described piston transfer block are slidably connected.
The bottom of described cylinder body is arranged with insulation covering to the upper end of described single rifle head, and described insulation covering is positioned at described conducting block place and is provided with opening, and described conducting block conducts electricity with described single rifle head and is connected.
Described piston upper end is arranged with seal cartridge, and is fixed by piston gland.
The lower end of described single rifle head is arranged with the 4th compression spring, and described 4th compression spring is located at below described insulation covering, and inconsistent with the lower end of described insulation covering.
Described single rifle module also comprises the second guide rail, the second slide block, the 3rd guide rail and the 3rd slide block, described second guide rail and the 3rd guide rail are all vertically located on described single rifle backboard, described second slide block is stuck on described second guide rail, and move up and down along the second guide rail, described 4th Timing Belt is connected with described second slide block, and described single rifle transfer block is connected with described second slide block; Described liquid feeding pin is located on described 3rd slide block, and the output terminal of described 3rd direct-current machine is connected with described 3rd slide block.
Described sample storehouse comprises base plate, some sample racks, sample rack fence, overhead door and drop protective shield, described sample rack fence is three fences, and be located on described plate upper surface, described overhead door is positioned at the opening part of described sample rack fence, and be rotationally connected with described base plate, it is inner that described sample rack inserts described sample rack fence from sample rack fence opening part, and described drop protective shield is located at above described sample rack, and frame is on described sample rack fence.
Described sample storehouse is positioned at the right side of described table top, nucleic acid extraction module is located at the postmedian of described table top, the left side of described nucleic acid extraction module is located in described reagent area, described table top is positioned at described nucleic acid extraction module and front portion, reagent area is provided with suction nozzle district, between described suction nozzle district and described sample storehouse, is provided with nucleic acid rest area.
Described suction nozzle district comprises suction nozzle storage area and suction nozzle abandons district, described suction nozzle abandons below district and is provided with trash receptacle, described trash receptacle side is provided with scavenging solution bucket and waste liquid barrel, described scavenging solution bucket and waste liquid barrel are connected with liquid feeding pin with the joint of described volley of rifle fire module respectively, and described suction nozzle storage area has deposited conduction suction nozzle and non-conductive suction nozzle.
Described volley of rifle fire module-external is provided with volley of rifle fire shield cap, and described single rifle module-external is provided with single rifle shield cap.
The beneficial effect that the present invention brings is:
In the present invention, described Full automatic instrument for extracting nucleic acid, it is the instrument that the supporting nucleic acid extracting reagent of application completes the extraction work of sample nucleic acid automatically, packaged matched reagent is utilized automatically to complete extraction purification process, plant and instrument running cost is low, easy to operate, just more and more applied.This instrument has, following advantage:
1. effective preventing pollution: volley of rifle fire module and single rifle module is anti-pollution walks, ensures that application of sample can not pollute; Instrument, with high efficiency particle air filter, effectively prevents atmospheric pollution; Online ultraviolet sterilization, band liquid waste treating apparatus, to greatest extent decreasing pollution; In addition, the drop protective shield design of sample storehouse, effectively prevents sample contamination.
2. real full-automatic: the distribution of sample pipetting volume, reagent, Beads enrichment and purifying are all completed by instrument, instrument internal integrated heating and concussion function fully automated, get rid of manual intervention.
3. rapid extraction: disposablely at most can process 48 tests, can process 150 tests every day.
4. accurately control, high extracting efficiency and DNA purity, intelligent wash pattern improve flush efficiency; Extraction efficiency improves in high-efficiency vibration mechanism; Nucleic acid extraction is made to reach high purity, high yield pulp1.
5. result is stablized: the difference avoiding manual operation to cause and mistake, and result temperature is reproducible; Can optimize purification scheme according to reagent, achieve higher extraction efficiency, the DNA/RNA purity of extraction is high, can be directly used in PCR and SAT.
6. high biological safety: Sample Preparation Procedure prosthetic is intervened, ensures the security of operator; Whole leaching process is completely closed, pollution-free to experimental situation.
7. safe and reliable, cost is low: close and extract storehouse, medical disposable material, at utmost reduce the contact of operator and reagent; In addition, the consumptive material in whole separate nucleic acid process is few, and cost is low.
8. freely programme: powerful program editing function; Define your application flexibly, efficiently, different reagent requirement can be met.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Fig. 1 is the structural representation of instrument for extracting nucleic acid of the present invention.
Fig. 2 is the structural representation that instrument for extracting nucleic acid of the present invention removes shell state.
Fig. 3 is the structural representation of nucleic acid extraction module of the present invention.
Fig. 4 is the structural representation that nucleic acid extraction module of the present invention removes shell state front.
Fig. 5 is the structural representation that nucleic acid extraction module of the present invention removes shell state reverse side.
Fig. 6 is the structural representation of heating arrangements of the present invention.
Fig. 7 is the sectional view of heating arrangements of the present invention.
Fig. 8 is the structural representation of magnetic suck mechanism of the present invention.
Fig. 9 is the explosive view of magnetic suck mechanism of the present invention.
Figure 10 is the structural representation of volley of rifle fire module of the present invention.
Figure 11 is the sectional view of volley of rifle fire module of the present invention.
Figure 12 is the structural representation in volley of rifle fire head group part front of the present invention.
Figure 13 is the structural representation of volley of rifle fire head group part reverse side of the present invention.
Figure 14 is the structural representation of single rifle module of the present invention.
Figure 15 is the structural representation of filling mechanism of the present invention.
Figure 16 is the structural representation that single rifle module of the present invention removes filling mechanism.
Figure 17 is the sectional view at single rifle buffer gear place of single rifle module of the present invention.
Figure 18 is the sectional view at the 4th guide rod place of single rifle module of the present invention.
Figure 19 is the structural representation of sample storehouse of the present invention.
Figure 20 is the explosive view of sample storehouse of the present invention.
The label that in figure, component names is corresponding is as follows:
1, shell; 2, frame; 3, nucleic acid extraction module; 31, housing; 32, base; 33, comb; 34, vibrating mechanism; 341, oscillating plate; 342, the first motor; 343, excentric shaft; 344, briquetting; 345, bearing; 346, the first synchronous pulley; 347, the first Timing Belt; 35, heating arrangements; 351, the first linear electric motors; 352, hot-plate; 353, heating piece; 354, pressing plate; 355, thermal baffle; 356, the first guide rod; 357, screw mandrel; 358, bottoming hole; 359, through hole; 360, insulating cotton; 36, magnetic suck mechanism; 361, magnet steel seat; 362, magnet steel; 363, the second motor; 364, magnet steel mounting groove; 365, magnet steel lid; 366, screw; 367, tail rod; 368, the second Timing Belt; 369, Timing Belt clamping piece; 37, air blowing fan; 38, drawing fan; 4, sample storehouse; 41, base plate; 42, sample rack; 43, sample rack fence; 44, overhead door; 45, drop protective shield; 5, volley of rifle fire module; 51, volley of rifle fire backboard; 52, the 3rd motor; 53, volley of rifle fire head group part; 531, volley of rifle fire head; 532, the rifle headstock; 533, fixed block; 534, volley of rifle fire buffer gear; 5341, the second guide rod; 5342, the second compression spring; 5343, the first linear bearings; 535, sliding sleeve; 536, the first compression spring; 537, joint; 538, non-conductive suction nozzle; 539, rifle head fixture block; 54, hoisting appliance; 541, the first screw rod; 542, nut; 543, motion-work wheel; 544, follow-up pulley; 545, head bearing; 546, the 3rd Timing Belt; 547, lower bearing; 548, head bearing frame; 549, the first set nut; 5410, lower bearing bracket; 5411, nut gland; 55, slide mechanism; 551, the first guide rail; 552, slide block; 6, single rifle module; 61, single rifle transfer block; 62, single rifle head; 63, piston; 64, cylinder body; 65, the second linear electric motors; 66, first circuit board; 67, piston transfer block; 68, insulate joint pin; 69, pneumatic fittings; 610, baroceptor; 611, conducting block; 612, conduction suction nozzle; 613, single rifle buffer gear; 6131, the 3rd guide rod; 6132, the 3rd compression spring; 6133, the second linear bearings; 6134, the second set nut; 6135, linear axis bearing sleeve; 614, the 4th guide rod; 615, insulation covering; 616, opening; 617, seal cartridge; 618, piston gland; 619, the 4th compression spring; 620, single rifle backboard; 621, filling mechanism; 6211, the 4th motor; 6212, liquid feeding pin; 6213, the 3rd linear electric motors; 6214, the 4th Timing Belt; 622, the second guide rail; 623, the second slide block; 624, the 3rd guide rail; 625, the 3rd slide block; 7, table top; 8, X arm; 9, volley of rifle fire Y arm; 10, single rifle Y arm; 11, nucleic acid rest area; 12, suction nozzle storage area; 13, suction nozzle abandons district; 14, trash receptacle; 15, scavenging solution bucket; 16, waste liquid barrel; 17, reagent area.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail:
As the embodiment of instrument for extracting nucleic acid of the present invention, as Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7, Fig. 8, Fig. 9, Figure 10, Figure 11, Figure 12, Figure 13, Figure 14, Figure 15, Figure 16, Figure 17, Figure 18, shown in Figure 19 and Figure 20, comprise shell 1, frame 2, nucleic acid extraction module 3, sample storehouse 4, reagent area 17, volley of rifle fire module 5 and single rifle module 6, described frame is provided with table top 7, described nucleic acid extraction module 3, sample storehouse 4 and reagent area 17 are all located on table top 7, described volley of rifle fire module 5 and single rifle module 6 are positioned at above described table top 7, described frame 2 is provided with X arm 8, described X arm 8 is provided with volley of rifle fire Y arm 9 and single rifle Y arm 10, described volley of rifle fire Y arm 9 and single rifle Y arm 10 slide along X arm 8, described volley of rifle fire module 5 is located on described volley of rifle fire Y arm 9, and slide along volley of rifle fire Y arm 9, described single rifle module 6 is located on described single rifle Y arm 10, and slide along single rifle Y arm 10.Described single rifle module 6 is for adding sample, moving sample and add scavenging solution, described volley of rifle fire module 5 is for detaching the waste liquid in comb, arranging of described X arm 8, volley of rifle fire Y arm 9 and single rifle Y arm 10 can make volley of rifle fire module 5 and the movement arbitrarily in the horizontal plane of single rifle module 6, and then realize anti-pollution walking, ensure that application of sample can not pollute, and add scavenging solution, detaching the operation such as waste liquid can not contaminated sample, ensure that precision and the purity of nucleic acid extraction.
In the present embodiment, described nucleic acid extraction module 3 comprises housing 31, base 32, comb 33, vibrating mechanism 34, heating arrangements 35 and magnetic suck mechanism 36, described comb comprises six reaction tubess linked together, described heating arrangements 35 is located on the upper surface of described base 32, described comb 33 is located on described vibrating mechanism 34, described vibrating mechanism 34 is positioned at above described heating arrangements 35, described magnetic suck mechanism 36 is between described heating arrangements 35 and described vibrating mechanism 34, and move along described heating arrangements 35 upper surface, described housing 31 to be located at above described base 32 and to be positioned at described vibrating mechanism 34, heating arrangements 35 and magnetic suck mechanism 36 outside.The high-efficiency vibration efficiency of described vibrating mechanism 34 improves extraction efficiency, described heating arrangements 35 realizes heating function, the sample in comb 33 is made to carry out incubation operation, described magnetic suck mechanism 36 attracts the magnetic-particle in comb 33 by magnetic field force, by volley of rifle fire module 5, remaining liquid in comb 33 is detached comb 33 again, and repeatedly clean.
In the present embodiment, described vibrating mechanism 34 involving vibrations plate 341, first motor 342, two excentric shafts 343 and two briquettings 344, described comb 33 is located on described oscillating plate 341, described first motor 342 is located on described base 32, two excentric shafts 343 are located at the two ends of described oscillating plate 341 respectively, described excentric shaft 343 is arranged with bearing 345, and compressed by briquetting 344, the upper end of described excentric shaft 343 and the output terminal of the first motor 342 are equipped with the first synchronous pulley 346, by the first Timing Belt 347, two excentric shafts 343 are connected with the first motor 342.Adopt the first motor 342 to drive excentric shaft 343 rotation to realize oscillating plate 341 to vibrate back and forth in the horizontal plane, play the effect of the liquid fully shaken up in comb 33, two excentric shafts 343 are adopted to ensure the levelness of oscillating plate 341 when vibrating, prevent the liquid in comb 33 from spilling, and then reduce the possibility of crossed contamination.
In the present embodiment, described heating arrangements 35 comprises the first linear electric motors 351, hot-plate 352 and heating piece 353, described heating piece 353 is located at below described hot-plate 352, and compressed by pressing plate 354, described hot-plate 352 upper surface is provided with thermal baffle 355, described hot-plate 352 two ends are respectively provided with first guide rod 356 downwards, the middle part of described hot-plate 352 is to having a rhizoid bar 357, two first guide rod 356 lower ends are through described base 32, first linear electric motors 351 are located on the lower surface of described base 32, described screw mandrel 357 lower end is through described base 32, and be connected with described first linear electric motors 351, described hot-plate 352 is provided with 48 bottoming holes 358, the status park that 48 bottoming holes 358 arrange according to six row eight, described thermal baffle 355 is provided with 48 through holes 359 with described bottoming hole 358 corresponding section, described hot-plate 352 and heating piece 353 outside are provided with one layer of heat preservation cotton 360.Described heating piece 353 adopts resistance heating, transfers heat to hot-plate 352, and in a heated state, the lower end of described comb 33 is absorbed in described bottoming hole 358, and therefore, the heat that heating piece 353 produces passes to the liquid in comb 33, and then realizes incubation; Described insulating cotton 360 plays the effect of insulation, prevents the heat loss that heating piece 353 produces.
In the present embodiment, described magnetic suck mechanism 36 comprises magnet steel seat 361, magnet steel 362 and the second motor 363, described magnet steel seat 361 is provided with 21 magnet steel mounting grooves 364, the status park that 21 magnet steel mounting grooves 364 arrange according to three row seven, described magnet steel 362 is located in described magnet steel mounting groove 364, and compressed by magnet steel lid 365, by screw 366, described magnet steel lid 365 is connected with described magnet steel seat 361, two tail rods be parallel to each other 367 are provided with between the internal surface that two of described housing 31 are corresponding, described magnet steel seat 361 is located on two tail rods 367, described second motor 363 is located on the internal surface of described housing 31, and be connected with described magnet steel seat 362 by the second Timing Belt 368, described second Timing Belt 368 is connected with described magnet steel seat 361 by Timing Belt clamping piece 369.After having lowered the temperature, second motor 363 running drive magnetic suck mechanism 36 is moved to the left and carries out magnetic suck, every movement in minute position, and then the magnetic suck operation not having an independent reaction tubes all to carry out three minutes of every root comb 33 can be ensured, the design of above magnetic suck mechanism 36 and motion make the adsorption time of every root comb 33 pipe consistent, and the otherness avoiding adsorption time length causes the impact on result; Carry out after three minutes taking out waste liquid, took out once every one minute afterwards.
In the present embodiment, two sides that described housing 31 is corresponding are respectively equipped with one group of air blowing fan 37 and one group of drawing fan 38, and described air blowing fan 37 and drawing fan 38 are symmetrically set.The discharge of housing 31 internal heat is convenient in the setting of described air blowing fan 37 and drawing fan 38.
In the present embodiment, described volley of rifle fire module 5 comprises volley of rifle fire backboard 51, the 3rd motor 52, volley of rifle fire head group part 53, hoisting appliance 54 and slide mechanism 55, described volley of rifle fire backboard 51 is fixed on described volley of rifle fire Y arm 9, the top of described volley of rifle fire backboard 51 is located at by described 3rd motor 52, and be connected with described hoisting appliance 54, described volley of rifle fire head group part 53 is slidably connected by slide mechanism 55 and described volley of rifle fire backboard 51, and realizes sliding up and down by hoisting appliance 54.The effect of described volley of rifle fire module 5 is that the waste liquid being used for cleaning magnetic bead in comb 33 is detached comb, and repeatedly detaches.
In the present embodiment, corresponding, described volley of rifle fire head group part 53 comprises six volley of rifle fire heads 531, the rifle headstock 532, fixed block 533 and volley of rifle fire buffer gear 534, described volley of rifle fire head 531 is vertical on the described rifle headstock 532, the middle part of described volley of rifle fire head 531 is arranged with sliding sleeve 535 and the first compression spring 536, described first compression spring 536 is positioned at above described sliding sleeve 535, and the upper end of described volley of rifle fire head 531 is arranged with joint 537, and upper end is arranged with non-conductive suction nozzle 538, the described rifle headstock 532 is connected with described fixed block 533 by rifle head fixture block 539, described volley of rifle fire buffer gear 534 comprises the second guide rod 5341 and the second compression spring 5342, described second guide rod 5341 upper end is connected with described fixed block 533, lower end is inserted in described rifle head fixture block 539, the lower end of described second guide rod 5341 is arranged with the first linear bearings 5343, described first linear bearings 5343 is between described second guide rod 5341 and described rifle head fixture block 539, described second compression spring 5342 is sheathed on described second guide rod 5341, and be positioned at above described first linear bearings 5343.Each volley of rifle fire head 531 on described volley of rifle fire assembly 53 can move up and down separately, when described volley of rifle fire head 531 moves downward the non-conductive suction nozzle 538 of loading, thus do not have the situation that indivedual volley of rifle fire head 531 fails to load non-conductive suction nozzle 538 when ensureing that the suction nozzle holding power of six volley of rifle fire heads 531 is close, also can avoid causing indivedual non-conductive suction nozzle 538 to load due to the difference of holding power firm not, the situation that non-conductive suction nozzle 538 drops described in the process used.
In the present embodiment, described hoisting appliance 54 comprises the first screw rod 541 and nut 542, described 3rd motor 52 output terminal is provided with motion-work wheel 543, described first screw rod 541 upper end is arranged with follow-up pulley 544 and two head bearings 545, described follow-up pulley 544 is connected with described motion-work wheel 543 by the 3rd Timing Belt 546, described first screw rod 541 lower end is arranged with a lower bearing 547, described head bearing 545 is fixed by head bearing frame 548, and lock with the first set nut 549, described lower bearing 547 is fixed by lower bearing bracket 5410, described nut 542 is sheathed on described first screw rod 541 middle and upper part, and it is inner to be positioned at described fixed block 533, fixed by nut gland 5411.The mode cooperatively interacted by the first screw rod 541 and nut 542 is realized volley of rifle fire head group part 53 and moves up and down, and this kind of mode of motion is relatively steady, and then ensure that the accuracy being at loading conduction suction nozzle 538, and loading is put in place more.
In the present embodiment, described slide mechanism 55 comprises the first guide rail 551 and slide block 552, and described first guide rail 551 is vertically located on described volley of rifle fire backboard 51, and described slide block 552 is located on described fixed block 533, described slide block 552 is stuck on described first guide rail 551, and slides up and down along the first guide rail 551.Described first guide rail 551 and slide block 552 ensure that the perpendicularity that volley of rifle fire head group part 53 moves up and down, and then ensure that the accuracy being at loading conduction suction nozzle 538, and loading is put in place more.
In the present embodiment, described single rifle module 6 comprises single rifle backboard 620, single rifle transfer block 61, single rifle head 62, piston 63, cylinder body 64, second linear electric motors 65, first circuit board 66 and filling mechanism 621, described liquid feeding structure 621 comprises the 4th motor 6211, liquid feeding pin 6212 and the 3rd linear electric motors 6213, described 4th motor 6211 drives described single rifle transfer block 61 to move up and down by the 4th Timing Belt 6214, and described 3rd linear electric motors 6213 drive liquid feeding pin 6212 to move up and down, described single rifle backboard 620 is fixed on described single rifle Y arm 10, the output terminal of described second linear electric motors 65 is connected by the upper end of piston transfer block 67 with described piston 63, it is inner that described cylinder body 64 is located in described piston 63 lower end, described single rifle head 62 upper end is connected with described cylinder body 64 lower end by insulation joint pin 68, and be connected with described cylinder body 64 inside, described cylinder body 64 is provided with pneumatic fittings 69, described pneumatic fittings 69 is connected with described cylinder body 64, described first circuit board 66 is provided with the baroceptor 610 be connected with described pneumatic fittings 69, described single rifle head 62 is provided with the conducting block 611 be connected with first circuit board 66, described second linear electric motors 65 are electrically connected with first circuit board 66, described single rifle head 62 lower end is provided with conduction suction nozzle 612.Sample after being extracted nucleic acid for being transplanted in comb 33 by the sample in sample storehouse 4, and is transferred to nucleic acid rest area 11 by single rifle head 62 of described single rifle module 6, and described liquid feeding structure 621 for adding scavenging solution in comb 33.Described baroceptor 610 can be used for detecting liquid level, when single rifle head 62 starts to move downward, described piston 63 is positioned at the upper end of described cylinder body 64, along with single rifle head 62 moves downward, described piston 63 moves downward simultaneously, gas then in cylinder body 64 is slowly discharged from the lower end of described single rifle head 62, when the lower end of single rifle head 62 touches liquid level, gas in described cylinder body 64 can not be got rid of for the moment, the air pressure in cylinder body 64 is then caused to rise, then described baroceptor 610 detects the air pressure signal risen suddenly, known single rifle head 62 has touched liquid level, first circuit board 66 records the height location of the rifle head 62 that places an order, first rising declines again carries out the operation of imbitition, liquid comprises sample, reagent etc., whether leakage after described baroceptor 610 also can be used for detecting in suction nozzle and draw liquid, when leakage, the air pressure in cylinder body can reduce suddenly, then baroceptor 610 detects the air pressure signal declined suddenly, the known generation having leakage situation, is convenient to user and carries out corrective operation in time.A voltage signal is produced when conduction suction nozzle 612 touches liquid level by described conduction suction nozzle 612 and conducting block 611, known single rifle head 62 has touched liquid level, first circuit board 66 records the height location of the rifle head 62 that places an order, and first rising declines again carries out the operation of imbitition.Baroceptor 610 can be selected to detect liquid level according to different test items or detect liquid level by conducting block 611.
In the present embodiment, described single rifle module 6 also comprises two groups of list rifle buffer gears 613, described single rifle buffer gear 613 comprises the 3rd guide rod 6131, 3rd compression spring 6132 and the second linear bearings 6133, the upper end of described 3rd guide rod 6131 is connected with described single rifle transfer block 61, lower end is connected with described cylinder body 64, and fixed by the second set nut 6134, described 3rd compression spring 6132 and the second linear bearings 6133 are all sheathed on described 3rd guide rod 6131, described 3rd compression spring 6132 is between described second linear bearings 6133 and described single rifle transfer block 61, described second linear bearings 6133 outer cover is provided with linear axis bearing sleeve 6135.When single rifle module 6 moves downward loading conduction suction nozzle 612, when holding power is too small, conduction suction nozzle 612 is easily caused to load firm not, appear in the process of use the situation that suction nozzle 612 drops of conducting electricity, described single rifle buffer gear 613 arrange can be suitable tune up holding power, because single rifle buffer gear 613 can cushion a part of surging force, and then effectively can ensure that conduction suction nozzle 612 is loaded on single rifle head 62.
In the present embodiment, vertical direction is provided with two the 4th guide rods 614 be parallel to each other between described piston transfer block 67 and described single rifle transfer block 61, described 4th guide rod 614 upper end is fixedly connected with described single rifle transfer block 61, and lower end and described piston transfer block 67 are slidably connected.Described 4th guide rod 614 ensure that the perpendicularity of piston up-down.
In the present embodiment, the bottom of described cylinder body 64 is arranged with insulation covering 615 to the upper end of described single rifle head 62, and described insulation covering 615 is positioned at described conducting block 611 place and is provided with opening 616, and described conducting block 611 conducts electricity with described single rifle head 62 and is connected.The lower end of described single rifle head 62 is arranged with the 4th compression spring 619, and described 4th compression spring 619 is located at below described insulation covering 615, and inconsistent with the lower end of described insulation covering 615.Described insulation covering 615 1 aspect can prevent from conducting electricity between conduction suction nozzle 612 and first circuit board 66 effectively, on the other hand, described insulation covering 615 also uses as sliding sleeve, when single rifle head 62 is loaded with conduction suction nozzle 612, described 4th compression spring 619 is compressed, and then impel described insulation covering 615 to move upward, when described insulation covering 615 moves upward to certain position, described first circuit board 66 is provided with position transducer, perceive the position of described insulation covering 615, and then judge that described single rifle head 62 is loaded with conduction suction nozzle 612.
In the present embodiment, described piston 63 upper end is arranged with seal cartridge 617, and is fixed by piston gland 618.Sealed by seal cartridge 617 and piston gland 618 pairs of cylinder bodies 64 and piston 63, this kind of sealing effectiveness is good, and does not affect piston 63 and move up and down in cylinder body 64.
Described single rifle module also comprises the second guide rail 622, second slide block 623, the 3rd guide rail 624 and the 3rd slide block 625, described second guide rail 622 and the 3rd guide rail 624 are all vertically located on described single rifle backboard 620, described second slide block 623 is stuck on described second guide rail 622, and move up and down along the second guide rail 622, described 4th Timing Belt 6214 is connected with described second slide block 623, and described single rifle transfer block 61 is connected with described second slide block 623; Described liquid feeding pin 6212 is located on described 3rd slide block 625, and the output terminal of described 3rd direct-current machine 6213 is connected with described 3rd slide block 625.Drive the 3rd slide block 625 to move up and down along the 3rd guide rail 624 by the rotation of the 3rd direct-current machine 6213, and then drive liquid feeding pin 6212 to move up and down; By the 4th motor 6211 and the 4th Timing Belt 6214 and then drive the second slide block 623 to move up and down along the second guide rail 622, described single rifle transfer block 61 is connected with described second slide block 623, then single rifle transfer block 61 moves up and down, then achieve single rifle head 62 and move up and down.
In the present embodiment, described sample storehouse 4 comprises base plate 41, three sample racks 42, sample rack fence 43, overhead door 44 and drop protective shields 45, described sample rack fence 43 is three fences, and be located on described base plate 41 upper surface, described overhead door 44 is positioned at the opening part of described sample rack fence 43, and be rotationally connected with described base plate 42, it is inner that described sample rack 42 inserts described sample rack fence 43 from sample rack fence 43 opening part, described drop protective shield 45 is located at above described sample rack 42, and frame is on described sample rack fence 43.Described sample rack 42 is positioned in described sample rack fence 43, and above sample rack 42, cover drop protective shield 45 can preventing pollution sample effectively, arranging of described overhead door 44 facilitates user to pick and place described sample rack 42.
In the present embodiment, described sample storehouse 4 is positioned at the right side of described table top 7, nucleic acid extraction module 3 is located at the postmedian of described table top 7, the left side of described nucleic acid extraction module 3 is located in described reagent area 17, described table top 7 is positioned at described nucleic acid extraction module 3 and front portion, reagent area 17 is provided with suction nozzle district, between described suction nozzle district and described sample storehouse 4, is provided with nucleic acid rest area 11.Above layout setting meets the movement locus of volley of rifle fire module 5 and single rifle module 6, and can prevent with regard to crossed contamination to the full extent, the arranging of described nucleic acid rest area 11 facilitate user to nucleic acid extraction well after sample preserve, be convenient to next step inspection.
In the present embodiment, described suction nozzle district comprises suction nozzle storage area 12 and suction nozzle abandons district 13, described suction nozzle abandons below district 13 and is provided with trash receptacle 14, described trash receptacle 14 side is provided with scavenging solution bucket 15 and waste liquid barrel 16, described scavenging solution bucket 15 and waste liquid barrel 16 are connected with liquid feeding pin 6212 with the joint 537 of described volley of rifle fire module 5 respectively, and described suction nozzle storage area 12 has deposited conduction suction nozzle 612 and non-conductive suction nozzle 538.Arranging of described suction nozzle storage area 12 facilitates user to deposit all kinds of suction nozzles required in nucleic acid extraction process, and the suction nozzle after use can be abandoned district 13 from described suction nozzle and be dropped in trash receptacle 14 by described volley of rifle fire module 5 and single rifle module 6 after using suction nozzle.
In the present embodiment, described volley of rifle fire module 5 outside is provided with volley of rifle fire shield cap (not shown), and described single rifle module 6 outside is provided with single rifle shield cap (not shown).Described volley of rifle fire shield cap and single rifle shield cap are respectively used to protection volley of rifle fire module 5 and single rifle module 6, prevent the erosion of reagent, sample or scavenging solution, and then improve work-ing life.
Certainly, described comb also can comprise four or five or seven or eight reaction tubess linked together, corresponding, and described volley of rifle fire head group part comprises four or five or seven or eight volley of rifle fire heads; Described hot-plate also can be provided with 24 or 36 or 72 or 96 bottoming holes, the status park that 24 bottoming holes arrange according to six row four, the status park that 72 bottoming holes arrange according to six row nine, the status park that 96 bottoming holes arrange according to eight row 12, corresponding, described thermal baffle is provided with 24 or 36 or 72 or 96 through holes with described bottoming hole corresponding section; Described magnet steel seat is provided with 14 or 28 or 35 magnet steel mounting grooves, the status park that 14 magnet steel mounting grooves arrange according to two row seven, the status park that 28 magnet steel mounting grooves arrange according to four lines seven, the status park that 35 magnet steel mounting grooves arrange according to the five-element seven; Also two or four or five sample racks can be provided with in described sample rack fence.So, also all within scope, repeat no longer one by one here.
Claims (20)
1. an instrument for extracting nucleic acid, it is characterized in that comprising shell, frame, nucleic acid extraction module, sample storehouse, reagent area, volley of rifle fire module and single rifle module, described frame is provided with table top, described nucleic acid extraction module, sample storehouse and reagent area are all located on table top, described volley of rifle fire module and single rifle module are positioned at above described table top, described frame is provided with X arm, described X arm is provided with volley of rifle fire Y arm and single rifle Y arm, described volley of rifle fire Y arm and single rifle Y arm slide along X arm, described volley of rifle fire module is located on described volley of rifle fire Y arm, and slide along volley of rifle fire Y arm, described single rifle module is located on described single rifle Y arm, and slide along single rifle Y arm.
2. instrument for extracting nucleic acid as claimed in claim 1, it is characterized in that described nucleic acid extraction module comprises housing, base, comb, vibrating mechanism, heating arrangements and magnetic suck mechanism, described heating arrangements is located on the upper surface of described base, described comb is located on described vibrating mechanism, described vibrating mechanism is positioned at above described heating arrangements, described magnetic suck mechanism is between described heating arrangements and described vibrating mechanism, and move along described heating arrangements upper surface, described housing to be located at above described base and to be positioned at described vibrating mechanism, heating arrangements and magnetic suck mechanism outside.
3. instrument for extracting nucleic acid as claimed in claim 2, it is characterized in that described vibrating mechanism involving vibrations plate, the first motor, two excentric shafts and two briquettings, described comb is located on described oscillating plate, described first motor is located on described base, two excentric shafts are located at the two ends of described oscillating plate respectively, described excentric shaft is arranged with bearing, and compressed by briquetting, the upper end of described excentric shaft and the output terminal of the first motor are equipped with the first synchronous pulley, are connected by two excentric shafts by the first Timing Belt with the first motor.
4. instrument for extracting nucleic acid as claimed in claim 3, it is characterized in that described heating arrangements comprises the first linear electric motors, hot-plate and heating piece, described heating piece is located at below described hot-plate, and compressed by pressing plate, described hot-plate upper surface is provided with thermal baffle, described hot-plate two ends are respectively provided with first guide rod downwards, the middle part of described hot-plate is to having a rhizoid bar, two first lower end of the guide rod are through described base, first linear electric motors are located on the lower surface of described base, described screw mandrel lower end is through described base, and be connected with described first linear electric motors, described hot-plate is provided with some bottoming holes, described thermal baffle is provided with some through holes with described bottoming hole corresponding section.
5. instrument for extracting nucleic acid as claimed in claim 4, it is characterized in that described magnetic suck mechanism comprises magnet steel seat, magnet steel and the second motor, described magnet steel seat is provided with some magnet steel mounting grooves, described magnet steel is located in described magnet steel mounting groove, and compressed by magnet steel lid, by screw, described magnet steel lid is connected with described magnet steel seat, two tail rods be parallel to each other are provided with between the internal surface that two of described housing are corresponding, described magnet steel seat is located on two tail rods, described second motor is located on the internal surface of described housing, and be connected with described magnet steel seat by the second Timing Belt, described second Timing Belt is connected with described magnet steel seat by Timing Belt clamping piece.
6. the instrument for extracting nucleic acid as described in any one of claim 1-5, it is characterized in that described volley of rifle fire module comprises volley of rifle fire backboard, the 3rd motor, volley of rifle fire head group part, hoisting appliance and slide mechanism, described volley of rifle fire backboard is fixed on described volley of rifle fire Y arm, the top of described volley of rifle fire backboard is located at by described 3rd motor, and be connected with described hoisting appliance, described volley of rifle fire head group part is slidably connected by slide mechanism and described volley of rifle fire backboard, and realizes sliding up and down by hoisting appliance.
7. instrument for extracting nucleic acid as claimed in claim 6, it is characterized in that described volley of rifle fire head group part comprises some volley of rifle fire heads, the rifle headstock, fixed block and volley of rifle fire buffer gear, described volley of rifle fire head is vertical on the described rifle headstock, the middle part of described volley of rifle fire head is arranged with sliding sleeve and the first compression spring, described first compression spring is positioned at above described sliding sleeve, the upper end of described volley of rifle fire head is arranged with joint, and upper end is arranged with non-conductive suction nozzle; The described rifle headstock is connected with described fixed block by rifle head fixture block, described volley of rifle fire buffer gear comprises the second guide rod and the second compression spring, described second guide rod upper end is connected with described fixed block, lower end is inserted in described rifle head fixture block, the lower end of described second guide rod is arranged with the first linear bearings, described first linear bearings is between described second guide rod and described rifle head fixture block, and described second compression spring is sheathed on described second guide rod, and is positioned at above described first linear bearings.
8. instrument for extracting nucleic acid as claimed in claim 7, it is characterized in that described hoisting appliance comprises the first screw rod and nut, described 3rd motor output end is provided with motion-work wheel, described first screw rod upper end is arranged with follow-up pulley and two head bearings, described follow-up pulley is connected with described motion-work wheel by the 3rd Timing Belt, described first screw rod lower end is arranged with a lower bearing, described head bearing is fixed by head bearing frame, and with the first set nut locking, described lower bearing is fixed by lower bearing bracket, described nut sleeve is located at described first screw rod middle and upper part, and it is inner to be positioned at described fixed block, fixed by nut gland.
9. instrument for extracting nucleic acid as claimed in claim 8, it is characterized in that described slide mechanism comprises the first guide rail and slide block, described first guide rail is vertically located on described volley of rifle fire backboard, described slide block is located on described fixed block, described slide block is stuck on described first guide rail, and slides up and down along the first guide rail.
10. instrument for extracting nucleic acid as claimed in claim 1, it is characterized in that described single rifle module comprises single rifle backboard, single rifle transfer block, single rifle head, piston, cylinder body, the second linear electric motors, first circuit board and filling mechanism, described liquid feeding structure comprises the 4th motor, liquid feeding pin and the 3rd linear electric motors, described 4th motor drives described single rifle transfer block to move up and down by the 4th Timing Belt, and described 3rd linear electric motors drive liquid feeding pin to move up and down, described single rifle backboard is fixed on described single rifle Y arm, the output terminal of described second linear electric motors is connected by the upper end of piston transfer block with described piston, described piston is located at described cylinder interior, described single rifle head upper end is connected with described cylinder body lower end by insulation joint pin, and be connected with described cylinder interior, described cylinder body is provided with pneumatic fittings, described pneumatic fittings is connected with described cylinder body, described first circuit board is provided with the baroceptor be connected with described pneumatic fittings, described single rifle head is provided with the conducting block be connected with first circuit board, described second linear electric motors are electrically connected with first circuit board, described single rifle head lower end is provided with conduction suction nozzle.
11. instrument for extracting nucleic acids as claimed in claim 10, it is characterized in that described single rifle module also comprises two groups of list rifle buffer gears, described single rifle buffer gear comprises the 3rd guide rod, 3rd compression spring and the second linear bearings, the upper end of described 3rd guide rod is connected with described single rifle transfer block, lower end is connected with described cylinder body, and fixed by the second set nut, described 3rd compression spring and the second linear bearings are all sheathed on described 3rd guide rod, described 3rd compression spring is between described second linear bearings and described single rifle transfer block, described second linear bearings outer cover is provided with linear axis bearing sleeve.
12. instrument for extracting nucleic acids as claimed in claim 11, it is characterized in that being provided with two the 4th guide rods be parallel to each other between described piston transfer block and described single rifle transfer block on vertical direction, described 4th guide rod upper end is fixedly connected with described single rifle transfer block, and lower end and described piston transfer block are slidably connected.
13. instrument for extracting nucleic acids as claimed in claim 12, it is characterized in that the bottom of described cylinder body is arranged with insulation covering to the upper end of described single rifle head, described insulation covering is positioned at described conducting block place and is provided with opening, and described conducting block conducts electricity with described single rifle head and is connected.
14. instrument for extracting nucleic acids as claimed in claim 13, be is characterized in that described piston upper end is arranged with seal cartridge, and are fixed by piston gland.
15. instrument for extracting nucleic acids as claimed in claim 14, it is characterized in that the lower end of described single rifle head is arranged with the 4th compression spring, described 4th compression spring is located at below described insulation covering, and inconsistent with the lower end of described insulation covering.
16. instrument for extracting nucleic acids as claimed in claim 15, it is characterized in that described single rifle module also comprises the second guide rail, the second slide block, the 3rd guide rail and the 3rd slide block, described second guide rail and the 3rd guide rail are all vertically located on described single rifle backboard, described second slide block is stuck on described second guide rail, and move up and down along the second guide rail, described 4th Timing Belt is connected with described second slide block, and described single rifle transfer block is connected with described second slide block; Described liquid feeding pin is located on described 3rd slide block, and the output terminal of described 3rd direct-current machine is connected with described 3rd slide block.
17. instrument for extracting nucleic acids as claimed in claim 1, it is characterized in that described sample storehouse comprises base plate, some sample racks, sample rack fence, overhead door and drop protective shield, described sample rack fence is three fences, and be located on described plate upper surface, described overhead door is positioned at the opening part of described sample rack fence, and be rotationally connected with described base plate, it is inner that described sample rack inserts described sample rack fence from sample rack fence opening part, described drop protective shield is located at above described sample rack, and frame is on described sample rack fence.
18. instrument for extracting nucleic acids as claimed in claim 1, it is characterized in that described sample storehouse is positioned at the right side of described table top, nucleic acid extraction module is located at the postmedian of described table top, the left side of described nucleic acid extraction module is located in described reagent area, described table top is positioned at described nucleic acid extraction module and front portion, reagent area is provided with suction nozzle district, between described suction nozzle district and described sample storehouse, is provided with nucleic acid rest area.
19. instrument for extracting nucleic acids as claimed in claim 18, it is characterized in that described suction nozzle district comprises suction nozzle storage area and suction nozzle abandons district, described suction nozzle abandons below district and is provided with trash receptacle, described trash receptacle side is provided with scavenging solution bucket and waste liquid barrel, described scavenging solution bucket and waste liquid barrel are connected with liquid feeding pin with the joint of described volley of rifle fire module respectively, and described suction nozzle storage area has deposited conduction suction nozzle and non-conductive suction nozzle.
20. instrument for extracting nucleic acids as claimed in claim 1, is characterized in that described volley of rifle fire module-external is provided with volley of rifle fire shield cap, and described single rifle module-external is provided with single rifle shield cap.
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Denomination of invention: Nucleic acid extractor Effective date of registration: 20200710 Granted publication date: 20170118 Pledgee: Agricultural Bank of China Limited by Share Ltd. Jiaxing science and Technology Branch Pledgor: JIAXING QUEST BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020330000469 |
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Date of cancellation: 20211126 Granted publication date: 20170118 Pledgee: Agricultural Bank of China Limited by Share Ltd. Jiaxing science and Technology Branch Pledgor: JIAXING QUEST BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020330000469 |