CN113564033A - PCR pretreatment contact-free workstation - Google Patents
PCR pretreatment contact-free workstation Download PDFInfo
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- CN113564033A CN113564033A CN202110597344.5A CN202110597344A CN113564033A CN 113564033 A CN113564033 A CN 113564033A CN 202110597344 A CN202110597344 A CN 202110597344A CN 113564033 A CN113564033 A CN 113564033A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
Abstract
The invention discloses a PCR pretreatment contact-free workstation, which comprises a sample placing frame arranged in a cabinet, wherein a plurality of sample tubes loaded with samples are placed on the sample placing frame, and the tops of the sample tubes are provided with openable upper covers; the mixing unit is arranged at the rear end of the sample placing rack and comprises a sample tube locking assembly and a mixed liquid collecting plate which can slide relatively, and the mixed liquid collecting plate is provided with a plurality of liquid placing holes; the first carrying unit is arranged above the sample placing rack; the first pipetting unit is arranged on one side of the mixing unit; the solution placing box is arranged at the tail end of the mixing unit; the second pipetting unit is arranged above the solution placing box; the second carrying unit is arranged on one side of the mixing unit, the sample and the solution can be automatically mixed in the whole process, the whole process is completed automatically, no manual contact is caused, the possible attached pollution in manual operation is avoided, meanwhile, a plurality of samples and the solution can be mixed at one time, and the device is suitable for batch requirements of laboratories.
Description
Technical Field
The invention relates to the technical field of detection equipment, in particular to a PCR pretreatment contact-free workstation.
Background
Polymerase Chain Reaction (PCR), is a molecular biology technique for amplifying specific DNA fragments, and is used for amplifying specific DNA fragments. PCR can be regarded as special DNA replication in vitro, and the biggest characteristic is that a trace amount of DNA can be greatly increased.
In the experimental process of PCR, the pretreatment of mixing a sample and a solution is often required, and then a corresponding experiment can be continuously carried out, while the mixing mode at present needs to manually mix the solution and the sample; however, such an approach has the following disadvantages: because the mixed liquid is used for experiments, the required amount of the mixed liquid is large, if the mixed liquid is mixed through manual operation, the mixed liquid is not only complicated, but also too slow, meanwhile, the mixed liquid cannot be guaranteed not to be polluted, the effective operation of the experiments is not facilitated, and the experiment requirements of related personnel cannot be met.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide the contact-free workstation for PCR pretreatment, which has a simple structure, can automatically and contactlessly mix a large amount of samples and solutions and meets the requirements of experimenters.
In order to achieve the purpose, the invention adopts the technical scheme that: the utility model provides a PCR pretreatment contact-free workstation, including locating in the rack:
the device comprises a sample placing frame, a plurality of sample tubes loaded with samples are placed on the sample placing frame, and an openable upper cover is arranged at the tops of the sample tubes;
the mixing unit is arranged at the rear end of the sample placing frame and comprises a sample tube locking assembly and a mixed liquid collecting plate which can slide relatively, and the mixed liquid collecting plate is provided with a plurality of liquid placing holes;
the first carrying unit is arranged above the sample placing frame and used for carrying the sample tube to the sample tube locking assembly and opening the upper cover;
the first pipetting unit is arranged on one side of the mixing unit and is used for transferring the sample into the mixed liquid collecting plate;
the solution placing box is arranged at the tail end of the mixing unit and used for placing a solution to be mixed;
the second pipetting unit is arranged above the solution placing box and used for conveying the solution into the solution placing hole to be mixed with the sample;
and the second carrying unit is arranged on one side of the mixing unit and is used for moving out the mixed liquid collecting plate after mixing.
Furthermore, the device also comprises a CCD code scanning unit, wherein the CCD code scanning unit is arranged between the sample placing rack and the sample tube locking assembly and is used for scanning the two-dimensional code on the sample tube and then loading the two-dimensional code into the control system.
Furthermore, the sample tube locking assembly comprises two locking blocks which can be clamped with each other, and when the two locking blocks are clamped, a containing groove for containing the sample tube is formed.
Furthermore, first transport unit includes the X axle module, be equipped with the Z axle module that can move in X axle direction on the X axle module, be equipped with the carriage that can move in Z axle direction on the Z axle module, be equipped with on the carriage and screw on the lid and press from both sides tight subassembly for open after pressing from both sides tight and will uncap the sample cell rotation.
Further, the first pipetting unit comprises:
the sliding seat can be arranged at the bottom of the cabinet in a movable mode in the X-axis direction and is provided with a plurality of first liquid-transfer gun heads;
and the automatic pipette tip assembling machine is arranged above the sliding seat, can move on X, Y and Z axis and is used for automatically assembling the first pipette tip.
Further, the second pipetting unit comprises a second pipette tip movable in X, Y and the Z axis.
Furthermore, the workstation also comprises a control panel used for controlling the workstation.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
the contact-free workstation for PCR pretreatment has a simple and compact structure, can automatically open an upper cover in a sample tube, adsorb the upper cover and then move the sample tube into the liquid containing hole of the mixed liquid collecting plate, simultaneously send the second pipette tip adsorption solution into the liquid containing hole of the mixed liquid collecting plate to be mixed with the sample, and fully automatically complete the whole process without any manual contact, thereby avoiding the possible additional pollution in manual operation, simultaneously mixing a plurality of samples with the solution once, having high mixing efficiency, being suitable for the batch requirements of laboratories, and having higher practicability and popularization value.
Drawings
The technical scheme of the invention is further explained by combining the accompanying drawings as follows:
FIG. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a schematic diagram of the internal structure of the present invention from another perspective;
FIG. 4 is a top view of the present invention;
wherein: the device comprises a cabinet 1, a sample placing frame 2, a mixing unit 3, a first carrying unit 4, a first pipetting unit 5, a second pipetting unit 6, a second carrying unit 7, a CCD code scanning unit 8, a control panel 9, a solution placing box 10, a sample tube 20, an upper cover 21, a clamping block 30, a mixed solution collecting plate 31, an X-axis module 40, a Z-axis module 41, a sliding frame 42, a screw cover clamping assembly 43, a pipetting tip automatic assembling machine 50, a first pipetting tip 51, a sliding seat 52 and a second pipetting tip 61.
Detailed Description
The invention is described in further detail below with reference to the figures and the embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not to be construed as limiting the invention.
Referring to fig. 1-4, a contact-free workstation for PCR pretreatment according to an embodiment of the present invention includes a sample holder 2, a mixing unit 3, a first transporting unit 4, a first pipetting unit 5, a second pipetting unit 6, a solution holding box 10, and a second transporting unit 7, which are disposed in a cabinet 1, wherein the sample in the sample holder 2 and the solution in the solution holding box 10 are mixed and then discharged through the cooperation of the mixing unit 3, the first transporting unit 4, the first pipetting unit 5, the second pipetting unit 6, and the second transporting unit 7, so that the whole process is performed in a contact-free manner, and a plurality of samples and solutions can be mixed at one time, and the mixing efficiency is high.
Specifically, the sample placing frame 2 has the functions of rotating and locking, so that the sample tube 20 can be rotated and locked, and the actual use is facilitated; in this embodiment, a plurality of sample tubes 20 carrying samples are placed on the sample holder 2, and an upper cover 21 which can be rotatably opened and closed is provided on the top of the sample tubes 20.
The mixing unit 3 is arranged at the rear end of the sample placing frame 2 and mainly serves as a workbench for bearing and mixing the sample and the solution; the mixing unit includes a sample tube locking assembly and a mixed solution collecting plate 31 that can slide laterally relative to each other in the X-axis direction.
The sample tube locking assembly comprises two clamping blocks 30 which can be mutually clamped through motor driving, when the two clamping blocks 30 are clamped, eight accommodating grooves capable of accommodating the sample tubes 20 are formed, and the sample tubes 20 can be conveyed into the accommodating grooves by the first conveying unit 4 and then clamped.
In addition, the mixed solution collecting plate 31 may also slide laterally on the X-axis, and the mixed solution collecting plate 31 has a plurality of solution placing holes for placing the mixed solution of the sample and the solution.
In practice, the screw-cap clamping assembly 43 comprises two clamping jaws that can be clamped to each other by means of a pneumatic cylinder, and the two clamping jaws can be rotated by means of a rotary pneumatic cylinder, so as to clamp the sample tube and unscrew the upper cap.
The first pipetting unit 5 comprises a pipetting head automatic assembly machine 50 and a sliding seat 52, the pipetting head automatic assembly machine 50 can move on X, Y and Z axis, the pipetting head automatic assembly machine 50 is positioned above the sliding seat 52, the sliding seat 52 can transversely move on X axis and is arranged at the bottom of the cabinet 1, and a plurality of first pipetting heads 51 matched with the pipetting head automatic assembly machine 50 are arranged on the sliding seat 52.
When liquid needs to be transferred, the sliding seat 52 drives the first liquid-transfer gun head 51 to move left to a set position, the automatic liquid-transfer gun head assembling machine 50 moves to the first liquid-transfer gun head 51 to automatically assemble the first liquid-transfer gun head 51, and then the first liquid-transfer gun head 51 sucks and opens the upper cover and then moves the sample in the sample tube back to the liquid placing hole of the mixed liquid collecting plate 31.
The solution placing cassette 10 is disposed on the distal end side of the mixing unit 3, the second pipetting unit 6 is disposed above the solution placing cassette 10, and the second pipetting unit 6 includes a second pipette tip 61 movable on X, Y and the Z-axis.
In operation, the second pipette tip 61 adsorbs the solution in the solution containing box 10 and then sends the solution to the solution containing hole of the mixed solution collecting plate 31 to mix with the sample.
The second carrying unit 7 is provided on the other side of the end of the mixing unit 3, and is configured to transfer the mixed liquid collecting plate 31 on which the mixed liquid is collected.
As a further preferred embodiment, the workstation further comprises a CCD code scanning unit 8, the sample tube 20 has a corresponding two-dimensional code, and the CCD code scanning unit 8 scans the two-dimensional code on the sample tube and loads the two-dimensional code into the control system for integral data recording.
As a further preferred embodiment, a control panel 9 for controlling the workstation is further arranged on the cabinet body 1, and the batch mixing operation of the samples and the solution can be realized by one key through related control buttons on the control panel 9.
The work flow of the workstation is as follows:
the first carrying unit conveys a plurality of sample tubes into the sample tube locking assembly to be clamped, and then the upper covers of the sample tubes are opened through the screw cap clamping assembly.
The automatic pipette tip assembling machine automatically assembles a plurality of first pipette tips, and then adsorbs samples in a plurality of sample tubes to the liquid placing holes of the mixed liquid collecting plate at one time through the first pipette tips.
And the plurality of second pipette tips adsorb the solution in the solution containing box to the solution containing holes of the mixed solution collecting plate to be mixed with the sample.
The second carrying unit removes the mixed liquid collecting plate after the sample and the solution are mixed.
The contact-free workstation for PCR pretreatment has a simple and compact structure, can automatically open an upper cover in a sample tube, adsorb the upper cover and then move the sample tube into the liquid containing hole of the mixed liquid collecting plate, simultaneously send the second pipette tip adsorption solution into the liquid containing hole of the mixed liquid collecting plate to be mixed with the sample, and fully automatically complete the whole process without any manual contact, thereby avoiding the possible additional pollution in manual operation, simultaneously mixing a plurality of samples with the solution at one time, having high mixing efficiency, being suitable for the batch requirements of laboratories, and having higher practicability and popularization value.
The above is only a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.
Claims (7)
1. The utility model provides a PCR pretreatment contact-free workstation, its characterized in that, including locating in the rack:
the device comprises a sample placing frame, a plurality of sample tubes loaded with samples are placed on the sample placing frame, and an openable upper cover is arranged at the tops of the sample tubes;
the mixing unit is arranged at the rear end of the sample placing frame and comprises a sample tube locking assembly and a mixed liquid collecting plate which can slide relatively, and the mixed liquid collecting plate is provided with a plurality of liquid placing holes;
the first carrying unit is arranged above the sample placing frame and used for carrying the sample tube to the sample tube locking assembly and opening the upper cover;
the first pipetting unit is arranged on one side of the mixing unit and is used for transferring the sample into the mixed liquid collecting plate;
the solution placing box is arranged at the tail end of the mixing unit and used for placing a solution to be mixed;
the second pipetting unit is arranged above the solution placing box and used for conveying the solution into the solution placing hole to be mixed with the sample;
and the second carrying unit is arranged on one side of the mixing unit and is used for moving out the mixed liquid collecting plate after mixing.
2. The contact-free workstation for PCR pretreatment according to claim 1, wherein: still include CCD and sweep a yard unit, CCD sweeps a yard unit and locates between sample rack and the sample cell locking Assembly for carry out the loading control system after scanning the two-dimensional code on the sample cell.
3. The contact-free workstation for PCR pretreatment according to claim 1, wherein: the sample tube locking assembly comprises two locking blocks which can be clamped mutually, and when the two locking blocks are clamped, a containing groove for containing a sample tube is formed.
4. The contact-free workstation for PCR pretreatment according to claim 1, wherein: the first carrying unit comprises an X-axis module, a Z-axis module capable of moving in the X-axis direction is arranged on the X-axis module, a sliding frame capable of moving in the Z-axis direction is arranged on the Z-axis module, and a screwing cover clamping assembly is arranged on the sliding frame and used for clamping the sample tube and opening the sample tube after the sample tube is opened.
5. The contact-free workstation for PCR pretreatment according to claim 1, wherein: the first pipetting unit comprises:
the sliding seat can be arranged at the bottom of the cabinet in a movable mode in the X-axis direction and is provided with a plurality of first liquid-transfer gun heads;
and the automatic pipette tip assembling machine is arranged above the sliding seat, can move on X, Y and Z axis and is used for automatically assembling the first pipette tip.
6. The contact-free workstation for PCR pretreatment according to claim 1, wherein: the second pipetting unit comprises a second pipette tip movable in X, Y and the Z axis.
7. The contact-free workstation for PCR pretreatment according to claim 1, wherein: and the control panel is used for controlling the workstation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110597344.5A CN113564033A (en) | 2021-05-31 | 2021-05-31 | PCR pretreatment contact-free workstation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110597344.5A CN113564033A (en) | 2021-05-31 | 2021-05-31 | PCR pretreatment contact-free workstation |
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| Publication Number | Publication Date |
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| CN113564033A true CN113564033A (en) | 2021-10-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110597344.5A Pending CN113564033A (en) | 2021-05-31 | 2021-05-31 | PCR pretreatment contact-free workstation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114797641A (en) * | 2022-04-21 | 2022-07-29 | 深圳市瑞吉生物科技有限公司 | Dispensing and transferring machine |
| CN114940375A (en) * | 2022-05-06 | 2022-08-26 | 深圳市瑞吉生物科技有限公司 | Heating synthesizer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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