CN111607500B

CN111607500B - Sampling device for detecting virus nucleic acid

Info

Publication number: CN111607500B
Application number: CN202010500389.1A
Authority: CN
Inventors: 马钰
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-04
Filing date: 2020-06-04
Publication date: 2023-06-27
Anticipated expiration: 2040-06-04
Also published as: CN111607500A

Abstract

The invention relates to a sampling device for detecting virus nucleic acid, which comprises a lower base station, an upper base station arranged above the lower base station and moving along an X axis, and a sample placing frame arranged above the X axis moving base station and moving along a Y axis; an X-axis sliding rail is arranged on the upper surface of the lower base station, and the upper base station is movably connected with the X-axis sliding rail; a Y-axis sliding rail is arranged on the upper surface of the upper base, and the sample placing rack is movably connected with the Y-axis sliding rail; a sealing cover is arranged on the lower base station; simple structure conveniently carries, protects through the sealed cowling when handling the sample that has biological hazard, has avoided the sample to cause the pollution to environment or experiment operating personnel, goes up the base station and can remove in the X axis direction, and the sample rack can remove in the Y axis direction, and then has guaranteed that the sample rack can carry out simultaneous movement in X axis and Y axis direction, can guarantee to carry out displacement treatment with the sample that can be accurate in the sample pipe with the sample rack when removing, has improved the efficiency of sample sampling.

Description

Sampling device for detecting virus nucleic acid

Technical Field

The invention relates to the technical field of virus nucleic acid detection, in particular to a sampling device for virus nucleic acid detection.

Background

Currently, nucleic acid detection methods are widely used for the detection of pathogenic microorganisms, and nucleic acid extraction is a key step of the detection method, especially for infectious diseases such as covd-19, and the sampling procedure of the step is extremely dangerous to experiment operators and environment due to high biological risks, so that the detection method must be completed in a biosafety cabinet of a P2/P3 laboratory.

At present, the nucleic acid extraction kits produced by most manufacturers are pre-packaged in 96-hole plates, when in use, the samples are only required to be added into the lysate holes of the pre-packaged plates for 5 minutes, and then the samples can be taken out from the safety cabinet after virus is completely inactivated, and the samples enter a machine extraction link, so that the subsequent steps have no biological hazard. Therefore, the biosafety cabinet is mainly used for biosafety protection before the specimen is added into the pre-assembled plate, however, in many infectious disease incidents, the outbreak treatment site is far away from a laboratory, even far away from a urban area, or the local laboratory does not have the biosafety cabinet, which brings difficulty to medical staff in rapidly diagnosing epidemic situation and checking infected persons on site.

For respiratory infectious diseases with stronger infectivity, such as COVID-19, the common detection process is to collect the exfoliated epithelial cells at the nasopharynx part by using a cotton swab, put the cells into a sampling tube filled with virus preservation solution, and make the cells on the surface of the cotton swab shed into the solution under the action of an oscillator, but a large amount of aerosol is easily generated in the process, and aerosol particles containing viruses overflow in a large amount when the cover is opened, so that the step is a key step causing pollution, and then the process of repeatedly sucking samples from the sampling tube by using a pipette is another aspect causing pollution.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention aims to provide a portable device which can also be used for carrying out the pretreatment of nucleic acid extraction in places other than laboratories, and a sampling device for detecting virus nucleic acid, which can effectively protect the environment and experimental operators by placing sample adding treatment of a biological dangerous sample in a closed environment.

In order to achieve the above purpose, the invention adopts the following technical scheme that the sampling device for detecting virus nucleic acid comprises a lower base station, an upper base station which is arranged above the lower base station and moves along an X axis, and a sample placing frame which is arranged above the X axis moving base station and moves along a Y axis;

an X-axis sliding rail is arranged on the upper surface of the lower base station, the upper base station is movably connected with the X-axis sliding rail, and the upper base station is driven to move along the X-axis direction by an X-axis driving device arranged between the upper base station and the lower base station;

the upper surface of the upper base is provided with a Y-axis sliding rail, the sample placing rack is movably connected with the Y-axis sliding rail, and the sample placing rack drives the sample placing rack to move along the Y-axis direction through a Y-axis driving device arranged between the sample placing rack and the upper base;

the lower base station is provided with a sealing cover, the upper base station and the sample placing rack are covered in the sealing cover by the sealing cover, and a round hole for inserting the sampling tube into the sealing cover is formed in the center of the top of the sealing cover.

The X-axis driving device comprises an X-axis threaded rod arranged below the upper base, and an X-axis threaded sleeve fixedly connected to the lower surface of the lower base, wherein the X-axis threaded sleeve is in threaded connection with the X-axis threaded rod, two ends of the X-axis threaded rod are movably connected with an X-axis threaded rod base arranged on the lower base, and one end of the X-axis threaded rod extends out of the sealing cover and is fixedly connected with the X-axis rotating button.

The Y-axis driving device comprises two Y-axis threaded rods which are arranged between the upper base and the sample placing frame and are parallel to each other, two ends of each Y-axis threaded rod are respectively and movably connected with a Y-axis threaded rod base fixedly connected with the upper base, and two sides of the lower surface of the sample placing frame are respectively provided with Y-axis threaded sleeves which are in threaded connection with the two Y-axis threaded rods;

the automatic sealing device is characterized by further comprising a driving rod, wherein one end of the driving rod extends out of the sealing cover and is fixedly connected with the Y-axis rotating button, the driving rod positioned in the sealing cover is movably connected with a driving rod base arranged on the upper base, two driving bevel gears are arranged on the driving rod and are respectively meshed with driven bevel gears arranged at the end parts of the two Y-axis threaded rods.

The X-axis rotating button and the Y-axis rotating button are positioned on the same side of the sealing cover.

An ultraviolet disinfection lamp is arranged in the sealing cover.

The sampling tube comprises a tube body, a necking part is arranged at the bottom of the tube body, an upper sealing cover is fixedly connected with the upper part of the tube body, a sawtooth ring with a sawtooth-shaped inner ring is fixedly arranged at the transition part between the tube body and the necking part, and a rotary sealing assembly for sealing an outlet of the necking part is fixedly arranged outside the necking part.

The rotary sealing assembly comprises an inner sleeve fixedly connected with the outside of the neck, an outer sleeve in threaded connection with the outside of the inner sleeve, an outer cylinder bottom cover with a circular through hole in the middle part is fixedly connected to the bottom of the outer sleeve, and an inward neck-shrinking end extending inwards is arranged at the lower part of the inner sleeve;

a first semicircular sealing cover and a second semicircular sealing cover are arranged between the bottom of the inner neck-shrinking end and the bottom cover of the outer cylinder;

one end of the diameter part of the first semicircular sealing cover is movably connected with the bottom of the inner neck-shrinking end through a first rotating shaft, and the other end of the diameter part of the first semicircular sealing cover is movably connected with the bottom cover of the outer cylinder through a second rotating shaft;

one end of the diameter part of the second sealing cover is movably connected with the bottom of the neck-in end through a third rotating shaft, and the other end of the diameter part of the second sealing cover is movably connected with the bottom cover of the outer cylinder through a fourth rotating shaft;

the first rotating shaft, the third rotating shaft, the second rotating shaft and the fourth rotating shaft are diagonally staggered;

when the outer sleeve is rotated, the outer sleeve drives the first semicircular sealing cover and the second semicircular sealing cover to rotate along the first rotating shaft and the third rotating shaft respectively, so that the first semicircular sealing cover and the second semicircular sealing cover are closed to seal the outlet of the necking part; or the first semicircular sealing cover and the second semicircular sealing cover are separated to communicate the outlet of the neck part with the circular through hole.

Rubber pads are arranged on the upper surfaces of the first semicircular sealing cover and the second semicircular sealing cover.

The rotary sealing assembly is sleeved with a lower sealing cover.

Sealing rings are arranged in the lower sealing cover and the upper sealing cover.

The beneficial effects of the invention are as follows: simple structure conveniently carries, protects through the sealed cowling when handling the sample that has biological hazard, has avoided the sample to cause the pollution to environment or experiment operating personnel, go up the base station and can remove in the X axis direction, the sample rack can remove in the Y axis direction, and then has guaranteed that the sample rack can carry out simultaneous movement in X axis and Y axis direction, can guarantee to handle the sample that samples in the sampling tube that can be accurate when removing with the sample rack and shift, has improved the efficiency of sample sampling.

Drawings

FIG. 1 is a schematic top view of the present invention;

FIG. 2 is a schematic side elevational view of the present invention;

FIG. 3 is a schematic view of the structure of a sampling tube according to the present invention.

FIG. 4 is a schematic view of the structure of the serrated ring in the coupon;

FIG. 5 is a schematic view of the seal assembly in the sampling tube;

FIG. 6 is a schematic view of the structure of the first semicircular lid and the second semicircular lid in the sampling tube;

FIG. 7 is a schematic illustration of the opening and closing of the first semicircular lid and the second semicircular lid in the sampling tube.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and examples.

In the description of the present invention, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.

The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second" may include one or more such features, either explicitly or implicitly; in the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.

Example 1

The sampling device for detecting viral nucleic acid shown in FIG. 1 comprises a lower base 1, an upper base 2 arranged above the lower base 1 and moving along the X axis, and a sample rack 3 arranged above the X axis moving base 2 and moving along the Y axis; the upper base station can be carried out along the X-axis direction of the lower base station 1, the sample placing frame 3 can be moved along the Y-axis direction of the upper base station, and further the sample placing frame can be ensured to be moved along the X-axis and Y-axis directions on the lower base station 1;

specifically, an X-axis sliding rail 5 is arranged on the upper surface of the lower base 1, the upper base 2 is movably connected with the X-axis sliding rail 5, and the upper base 2 drives the upper base 2 to move along the X-axis direction through an X-axis driving device arranged between the upper base 2 and the lower base 1; the two X-axis sliding rails 5 are parallel to each other, and the bottom of the upper base station 2 is provided with a sliding block matched with the X-axis sliding rails, so that the upper base station 2 is more stable when moving on the lower base station in the X-axis direction;

the upper surface of the upper base 2 is provided with a Y-axis sliding rail 6, the sample placing rack 3 is movably connected with the Y-axis sliding rail 6, and the sample placing rack 3 drives the sample placing rack 3 to move along the Y-axis direction through a Y-axis driving device arranged between the sample placing rack 3 and the upper base 2; the two Y-axis sliding rails 6 are crossed in the same plane with the X-axis sliding rails 5, the sample placing rack 3 is used for placing a nucleic acid extraction kit (a pre-filling reagent is usually a 96-well plate, and the use of the 96-well plate for sampling and cracking treatment is also recommended during manual extraction), sliding blocks which are movably connected with the Y-axis sliding rails in a matched manner are respectively arranged on two sides of the sample placing rack 3, so that the sample placing rack 3 can perform stable movement on an upper base, and further the sample placing rack can perform stable movement in the X-axis and Y-axis directions;

the lower base 1 is provided with a sealing cover 4, the sealing cover 4 covers the upper base 2 and the sample placing frame 3 into the sealing cover 4, and a round hole for inserting the sampling tube 7 into the sealing cover 4 is formed in the top of the sealing cover 4. The sealing cover 4 is arranged on the lower base 1, can seal and cover the upper base and the sample placing frame, ensures that a sample with biological danger in the sample placing frame cannot be diffused into the environment, protects the environment and staff from infection, is provided with a round hole at the top, can ensure that the sampling tube 7 is inserted into the sealing cover 4, guides sample liquid in the sampling tube 7 into a hollow groove of a reagent box on the sample placing frame 3, is provided with a sliding sealing cover, seals the round hole through the sealing cover after sampling is completed, ensures that the whole sealing cover is in a sealing state, and further protects the environment and the staff;

an ultraviolet disinfection lamp 13 is arranged in the sealing cover 4, and after detection is completed, the ultraviolet disinfection lamp is started to disinfect the whole device, so that cross infection is avoided.

Further, as shown in fig. 1 and 2, the X-axis driving device includes an X-axis threaded rod 8 disposed below the upper base 2, and an X-axis threaded sleeve 9 fixedly connected to the lower surface of the lower base 1, where the X-axis threaded sleeve 9 is in threaded connection with the X-axis threaded rod 8, two ends of the X-axis threaded rod 8 are movably connected with an X-axis threaded rod base 14 disposed on the lower base 1, and one end of the X-axis threaded rod 8 extends out of the seal cover 4 and is fixedly connected with an X-axis rotation button 20. When the X-axis movement is carried out, the X-axis rotating button is rotated to drive the X-axis threaded rod 8 to rotate, and the X-axis threaded sleeve 9 is fixedly connected to the lower surface of the lower base station 2 and is in threaded connection with the X-axis threaded rod 8, so that when the X-axis threaded rod 8 is rotated, the X-axis threaded sleeve 9 moves on the X-axis threaded rod, and further the upper base station is ensured to move in the X-axis direction on the lower base station;

the Y-axis driving device comprises two mutually parallel Y-axis threaded rods 11 arranged between the upper base 2 and the sample placing frame 3, two ends of the Y-axis threaded rods 11 are respectively and movably connected with a Y-axis threaded rod base 17 fixedly connected with the upper base 2, and two sides of the lower surface of the sample placing frame 3 are respectively provided with a Y-axis threaded sleeve 12 in threaded connection with the two Y-axis threaded rods 11; the Y-axis threaded sleeve is in threaded connection with the Y-axis threaded rod, so that the Y-axis threaded rod can drive the sample placing rack to move in the Y-axis direction on the upper base station when rotating, and the sample placing rack can move in the X-axis direction and the Y-axis direction;

specifically drive sample rack carry out Y axle motion on last base station, still include actuating lever 10, this actuating lever 10's one end extends outside the sealed cowling 4 with Y axle rotation button 19 fixed connection, the actuating lever 10 that is located the sealed cowling 4 is equipped with actuating lever base 18 swing joint on last base station 2, be equipped with two initiative bevel gears 15 on the actuating lever 10, these two initiative bevel gears 15 respectively with establish the driven bevel gear 16 meshing that is equipped with at two Y axle threaded rod 11 tip. The driving bevel gear 15 and the driven bevel gear 16 are meshed to change the transmission direction of force, specifically, the driving rod 10 is driven to rotate by rotating the Y-axis rotating knob 19 when rotating, and the driving rod 10 synchronously drives the Y-axis threaded rod 11 to rotate after being converted by the driving bevel gear and the driven bevel gear in the rotating process, so that the sample placing rack is synchronously driven to move;

in order to ensure convenient operation of the X-axis rotation knob 20 and the Y-axis rotation knob 19, the X-axis rotation knob 20 and the Y-axis rotation knob 19 are positioned on the same side of the seal cover 4. The operation is more convenient, and the X-axis moving scale and the Y-axis moving scale are arranged on the X-axis rotating button 20 and the Y-axis rotating button 19, so that the accuracy in adjustment is ensured.

Example 3

The sampling tube shown in fig. 3 comprises a tube body 701, a necking part 702 is arranged at the bottom of the tube body 701, an upper sealing cover 705 is fixedly connected with the upper part of the tube body 701, a sawtooth ring 704 with a sawtooth-shaped inner ring is fixedly arranged at the transition part between the tube body 701 and the necking part 702, and a rotary sealing assembly 703 for sealing the outlet of the necking part 702 is fixedly arranged outside the necking part 702. The tube 701 is made of a slightly soft material, so that the tube is extruded, the mixed solution of cells and solution collected in the tube is dripped into a hole groove of a 96-well plate of a kit in a sampling device, the aperture size of a sharp nozzle small opening at the bottom of the necking part 702 is 1mm, the area of liquid drops dripped out through the hole is 50ul, the joint of the tube 1 and the upper sealing cover 705 and the necking part 702 are made of hard materials, and the rotary sealing assembly and the upper sealing cover connected with the necking part are ensured to be stably connected with the tube; as shown in FIG. 4, the serration ring 704 has an integral structure between the outer ring of the serration ring 704 and the inner wall of the tube 701, and the serration arranged in the serration ring 704 can drop off the cells on the collecting cotton swab, so that the cells on the cotton swab can be introduced into the liquid of the tube through friction, and the danger of aerosol overflow caused by the falling off of the cells by using the oscillator is effectively avoided. And the protection to staff and environment is effectively caused.

Example 4

On the basis of embodiment 1, as shown in fig. 5, 6 and 7, the rotary sealing assembly 703 comprises an inner sleeve 7031 fixedly connected with the outside of the neck portion 702, an outer sleeve 7032 externally screwed with the inner sleeve 7031, and the inner sleeve 7032 and the outer sleeve can be rotatably connected, wherein the bottom of the outer sleeve 7032 is fixedly connected with an outer cylinder bottom cover 7033 with a circular through hole 7034 in the middle, and an inward neck-shrinking end 7035 extending inwards is arranged at the lower part of the inner sleeve 7031; the inner sleeve 7031 and the necking part 702 are in an integrated structure, the inner necking end 7035 is arranged to ensure that the mechanism of the whole rotary sealing assembly 703 is more stable, and the circular through hole 7034 is communicated with the small opening of the necking part when being opened to ensure that liquid in the neck tube body drips out;

a first semicircular sealing cover 7036 and a second semicircular sealing cover 7037 are arranged between the bottom of the inner neck-in end 7035 and the outer barrel bottom cover 7033; the first semicircular sealing cover and the second semicircular sealing cover are used for closing and opening the small opening and the round hole of the neck shrink, when the first semicircular sealing cover and the second semicircular sealing cover are opened, the small opening and the round hole of the neck shrink are opened, and liquid in the tube body is dripped into the empty slot of the 96-pore plate of the kit through the small opening and the round hole of the neck shrink;

when the first semicircular cover 7036 and the second semicircular cover 7037 are specifically connected, as shown in fig. 4 and fig. 5, one end of the diameter portion of the first semicircular cover 7036 is movably connected with the bottom of the neck-in end 7035 through a first rotating shaft 7038, and the other end is movably connected with the outer cylinder bottom cover 7033 through a second rotating shaft 7039;

one end of the diameter part of the second cover 7036 is movably connected with the bottom of the inner neck-shrinking end 7035 through a third rotating shaft 70311, and the other end of the diameter part of the second cover is movably connected with the outer barrel bottom cover 7033 through a fourth rotating shaft 70310;

the first rotating shaft 7038, the third rotating shaft 70311, the second rotating shaft 7039 and the fourth rotating shaft 70310 are diagonally staggered;

when the outer sleeve 7032 is rotated, the outer sleeve 7032 drives the first semicircular cover 7036 and the second semicircular cover 7037 to rotate along the first rotating shaft 7038 and the third rotating shaft 70311, so that the first semicircular cover 7036 and the second semicircular cover 7037 are closed to seal the outlet of the necking portion 702; or the first and second

semicircular caps

7036 and 7037 are separated to communicate the outlet of the constriction 702 with the circular through-hole 7034.

The outer sleeve 7032 is rotated to drive the first semicircular sealing cover and the second semicircular sealing cover to rotate anticlockwise as shown in fig. 7, at this time, because the bottom of the inner sleeve is rotationally connected with one side of the diagonal line of the first semicircular sealing cover and the second semicircular sealing cover, the diagonal line of the diameter of the outer cylinder bottom cover 7033 arranged at the lower end of the outer sleeve is rotationally connected with the first semicircular sealing cover and the second semicircular sealing cover, when the outer sleeve rotates anticlockwise, the corresponding sides of the first semicircular sealing cover and the second semicircular sealing cover are driven to rotate outwards, the small opening of the neck shrinkage part at the bottom of the tube body is communicated with the round hole of the outer cylinder bottom cover at the bottom of the outer sleeve, liquid in the tube body is extruded into the kit, after the extrusion of sample liquid is completed, the outer cylinder body is rotated clockwise, at this time, the inner sides of the corresponding sides of the first semicircular sealing cover and the second semicircular sealing cover are combined, the small opening of the neck shrinkage part of the tube body is closed with the round hole, and the liquid in the tube body is prevented from leaking outwards; the process of dripping the sample into the sampling tube is strictly ensured to be completely free from leakage, and the environment and operators are protected. Further, in order to ensure better sealing properties of the first semicircular cover and the second semicircular cover after butt joint, rubber pads are provided on the upper surfaces of the first semicircular cover 7036 and the second semicircular cover 7037.

Further, in order to ensure the tightness of the whole pipe body in use, a lower sealing cover 707 is sleeved outside the rotary sealing assembly 703; seal rings 706 are arranged in the lower seal cover 707 and the upper seal cover 705.

The middle part of the tube 7031 is made of softer materials, the mixture of epithelial cells collected in the tube and preservation solution can be extruded into a hole groove of a reagent box 96-hole plate on a sample placing rack through extruding the tube, the top of the tube 7031, an upper sealing cover 7033 and a lower end necking end are made of hard materials, the top and the necking end of the tube 7031 can be guaranteed to be in sealing connection with the upper sealing cover and the lower sealing cover through threads, an upper sealing ring and a lower sealing ring arranged in the upper sealing cover and the lower sealing cover can be in contact with an inlet at the upper end of the tube and an outlet at the necking end at the lower end of the tube, tightness of the tube is guaranteed, a sawtooth ring 704 arranged at the necking end in the tube 1 can scrape the epithelial cells collected through the cotton swab into the necking end of the tube to be mixed with the preservation solution, and the sawtooth ring 704 can scrape the cells on the cotton swab into the preservation solution through friction, and the top of the cotton swab can be sawn off to be stored in the tube 701.

In the implementation process, the aperture size of the sharp mouth small opening of the collecting tube is 1mm, so that the volume of each liquid drop passing through the hole is 50ul;

after collecting epithelial cells, the cotton swab of the sampling tube can rub on the sawtooth ring at the necking part, and the cells fall into the preservation solution through friction, so that the cotton swab can be directly preserved in the tube body. The sampling tube cover is screwed and sealed after the sampling cotton swab is put into the sampling tube cover. If the sample is required to be further vibrated and mixed, the tube body can be directly vibrated, and the risk of aerosol overflow is avoided.

The lid of tip mouth of body bottom of sampling pipe can unscrew before the application of sample, inserts the sampling pipe in the sealed cover application of sample round hole, then rotatory body 701 drives inner skleeve 7031 and rotates, and tip mouth is opened, drops quantitative liquid drop into the hole groove of kit 96 orifice plate through extrusion body (flexible), after the application of sample is accomplished, reverse direction rotatory body 701 closes tip mouth, again proposes the round hole with the sampling pipe.

The operation table can be used for opening ultraviolet disinfection before use.

X, Y the position of target hole can be adjusted to the slide rail knob, X knob clockwise rotation 30, X slide rail drives the reagent box frame and slides 4.5mm to right parallel, the row center spacing of a 96 orifice plate promptly, Y knob clockwise rotation 45, Y slide rail drives the reagent box frame and upwards slides 4.5mm parallel, a line center spacing promptly, after adjusting reagent box frame position, with target hole alignment seal cover loading hole, unscrew sampling pipe sharp mouth lid, vertically insert in the loading hole downwards, mark the degree of depth that gets into sealed lid on the body, this degree of depth can guarantee that the sampling pipe gets into the position of 3-5mm below the 96 orifice plate upper surface of reagent box, can not produce the liquid droplet splash when adding.

After the sample adding process is completed, the sample is mixed with the extracting reagent, the sample containing pathogenic microorganisms loses infectivity after contacting with the lysate, and the sealing cover is opened, so that the sample can be taken out from the operation table and put into a nucleic acid extractor for subsequent operation or further manual extraction is carried out.

The above embodiments are merely illustrative of the present invention and are not to be construed as limiting the scope of the present invention, and all designs which are the same or similar to the present invention are within the scope of the present invention.

Claims

1. The sampling device for detecting virus nucleic acid is characterized by comprising a lower base (1), an upper base (2) arranged above the lower base (1) and moving along an X axis, and a sample placing frame (3) arranged above the upper base (2) along a Y axis;

an X-axis sliding rail (5) is arranged on the upper surface of the lower base station (1), the upper base station (2) is movably connected with the X-axis sliding rail (5), and the upper base station (2) drives the upper base station (2) to move along the X-axis direction through an X-axis driving device arranged between the upper base station (2) and the lower base station (1);

the upper surface of the upper base (2) is provided with a Y-axis sliding rail (6), the sample placing rack (3) is movably connected with the Y-axis sliding rail (6), and the sample placing rack (3) drives the sample placing rack (3) to move along the Y-axis direction through a Y-axis driving device arranged between the sample placing rack (3) and the upper base (2);

a sealing cover (4) is arranged on the lower base table (1), the sealing cover (4) covers the upper base table (2) and the sample placing rack (3) into the sealing cover (4), and a round hole for inserting a sampling tube (7) into the sealing cover (4) is formed in the center of the top of the sealing cover (4);

the sampling tube (7) comprises a tube body (701), wherein a necking part (702) is arranged at the bottom of the tube body (701), and an upper sealing cover (705) is fixedly connected with the upper part of the tube body (701), and the sampling tube is characterized in that a sawtooth ring (704) with a sawtooth-shaped inner ring is fixedly arranged at the transition part between the tube body (701) and the necking part (702), and a rotary sealing assembly (703) for sealing an outlet of the necking part (702) is fixedly arranged outside the necking part (702);

the rotary sealing assembly (703) comprises an inner sleeve (7031) fixedly connected with the outside of the neck (702), an outer sleeve (7032) is connected with the outer thread of the inner sleeve (7031), an outer cylinder bottom cover (7033) with a circular through hole (7034) is fixedly connected to the bottom of the outer sleeve (7032), and an inward neck-shrinking end (7035) extending inwards is arranged at the lower part of the inner sleeve (7031);

a first semicircular sealing cover (7036) and a second semicircular sealing cover (7037) are arranged between the bottom of the inner neck-shrinking end (7035) and the outer barrel bottom cover (7033);

one end of the diameter part of the first semicircular cover (7036) is movably connected with the bottom of the inner neck-shrinking end (7035) through a first rotating shaft (7038), and the other end of the diameter part of the first semicircular cover is movably connected with the outer barrel bottom cover (7033) through a second rotating shaft (7039);

one end of the diameter part of the second semicircular sealing cover (7037) is movably connected with the bottom of the inward neck-shrinking end (7035) through a third rotating shaft (70311), and the other end of the diameter part of the second semicircular sealing cover is movably connected with the outer cylinder bottom cover (7033) through a fourth rotating shaft (70310);

the first rotating shaft (7038) and the third rotating shaft (70311), the second rotating shaft (7039) and the fourth rotating shaft (70310) are arranged in a diagonally staggered mode;

when the outer sleeve (7032) is rotated, the outer sleeve (7032) drives the first semicircular sealing cover (7036) and the second semicircular sealing cover (7037) to rotate along the first rotating shaft (7038) and the third rotating shaft (70311) respectively, so that the first semicircular sealing cover (7036) and the second semicircular sealing cover (7037) are closed to seal the outlet of the necking part (702); or the first semicircular lid (7036) and the second semicircular lid (7037) are separated to communicate the outlet of the neck (702) with the circular through hole (7034).

2. The sampling device for detecting virus nucleic acid according to claim 1, wherein the X-axis driving device comprises an X-axis threaded rod (8) arranged below the upper base (2), and an X-axis threaded sleeve (9) fixedly connected to the lower surface of the lower base (1), the X-axis threaded sleeve (9) is in threaded connection with the X-axis threaded rod (8), two ends of the X-axis threaded rod (8) are movably connected with an X-axis threaded rod base (14) arranged on the lower base (1), and one end of the X-axis threaded rod (8) extends out of the sealing cover (4) and is fixedly connected with an X-axis rotating knob (20).

3. The sampling device for detecting virus nucleic acid according to claim 2, wherein the Y-axis driving device comprises two mutually parallel Y-axis threaded rods (11) arranged between the upper base (2) and the sample placing frame (3), two ends of the Y-axis threaded rods (11) are respectively and movably connected with a Y-axis threaded rod base (17) fixedly connected with the upper base (2), and two sides of the lower surface of the sample placing frame (3) are respectively provided with a Y-axis threaded sleeve (12) in threaded connection with the two Y-axis threaded rods (11);

still include actuating lever (10), the one end of this actuating lever (10) extends outside sealed cowling (4) and Y axle swivel button (19) fixed connection, actuating lever (10) that are located in sealed cowling (4) are equipped with actuating lever base (18) swing joint on last base station (2), be equipped with two initiative bevel gears (15) on actuating lever (10), these two initiative bevel gears (15) mesh with driven bevel gear (16) that establish respectively that are equipped with at two Y axle threaded rod (11) tip.

4. A sampling device for the detection of viral nucleic acids according to claim 3, characterized in that the X-axis rotation knob (20) and the Y-axis rotation knob (19) are located on the same side of the sealing cap (4).

5. The sampling device for detecting viral nucleic acid according to claim 1, wherein an ultraviolet disinfection lamp (13) is provided within the sealed enclosure (4).

6. The sampling device for viral nucleic acid detection according to claim 1, wherein the upper surfaces of the first semicircular lid (7036) and the second semicircular lid (7037) are provided with rubber pads.

7. The sampling device for the detection of viral nucleic acids according to claim 1, wherein the rotary seal assembly (703) is sheathed with a lower seal cap (707).

8. The sampling device for the detection of viral nucleic acids according to claim 7, wherein sealing rings (706) are provided in the lower sealing cap (707) and the upper sealing cap (705).