CN113684120A - Nucleic acid detection apparatus and nucleic acid detection method - Google Patents
Nucleic acid detection apparatus and nucleic acid detection method Download PDFInfo
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- CN113684120A CN113684120A CN202110964695.5A CN202110964695A CN113684120A CN 113684120 A CN113684120 A CN 113684120A CN 202110964695 A CN202110964695 A CN 202110964695A CN 113684120 A CN113684120 A CN 113684120A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
Abstract
The present invention relates to a nucleic acid detecting apparatus and a nucleic acid detecting method, the nucleic acid detecting apparatus including: installing a base; the reagent strip mounting position is arranged on the mounting base and is used for mounting reagent strips containing reaction tubes; the centrifugal device is arranged on the mounting base and is used for centrifuging the sample in the reaction tube; the detection device is arranged on the mounting base and is used for carrying out amplification and fluorescence detection analysis on the sample in the reaction tube; the liquid-transfering device is arranged on the mounting base and used for moving the reaction tube. The nucleic acid detection equipment integrates a centrifugal device, a sample which finishes reaction in the reagent strip can be transferred to the centrifugal device through the liquid transfer device, and after centrifugation is finished under the action of the centrifugal device, the sample is transferred to the detection device through the liquid transfer device for detection. In the detection process, the reaction tube does not need to be manually taken out from the reagent strip and put into an additionally arranged centrifugal machine for centrifugation, so that manual operation is omitted, the detection efficiency of nucleic acid detection is improved, and the experiment cost is saved.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to nucleic acid detection equipment and a nucleic acid detection method.
Background
At present, molecular detection technology has been widely used in the research of infectious disease diagnosis, epidemic disease investigation, food hygiene inspection, early diagnosis of tumor and genetic diseases, forensic identification, tumor individualized diagnosis, blood screening, prenatal screening, pharmacogenomics and other fields, and is the most rapidly developed clinical examination technology at present.
The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and is widely applied to molecular biology detection and analysis due to its characteristics of strong specificity, high sensitivity, low purity requirement, simplicity and rapidness. However, the cumbersome nature of sample extraction and aerosol contamination are important barriers limiting further application of PCR technology to the clinic. Meanwhile, the traditional product has many experimental steps, low automation degree, needs a special PCR laboratory and other factors, and can restrict the rapid development and the primary clinical application of the product.
Although an integrated instrument platform claiming to be capable of performing nucleic acid detection fully automatically appears at present, due to structural limitations, an external centrifugal device still needs to be configured independently, after a nucleic acid sample is added into a reaction tube, an operator needs to manually take out the reaction tube, load the reaction tube on the centrifugal device, centrifuge the reaction tube so that the nucleic acid sample is filled into a detection cavity at the bottom of the reaction tube, and then manually transfer the reaction tube to a detection module for amplification and optical detection. Therefore, the operation of the existing integrated instrument platform for nucleic acid detection is still more complicated, so that the experimental efficiency is reduced, and the increasingly developed clinical requirements cannot be met at present.
Disclosure of Invention
In view of the above, it is necessary to provide a nucleic acid detecting apparatus and a nucleic acid detecting method which can achieve the technical effect of automatically centrifuging a reaction tube, in order to solve the problem that a sample in an integrated instrument platform for nucleic acid detection requires manual centrifugation.
According to an aspect of the present application, there is provided a nucleic acid detecting apparatus including:
installing a base;
the reagent strip mounting position is arranged on the mounting base and is used for mounting reagent strips containing reaction tubes;
the centrifugal device is arranged on the mounting base and is used for centrifuging the sample in the reaction tube;
the detection device is arranged on the mounting base and is used for carrying out amplification and fluorescence detection analysis on the sample in the reaction tube; and
the liquid transferring device is arranged on the mounting base and used for moving the reaction tube.
In one embodiment, the centrifuge device comprises:
the driving assembly is arranged on the mounting base;
the rotating piece is connected to the driving assembly in a matching mode, and is driven by the driving assembly to rotate around a rotating axis; and
the centrifugal piece is rotatably connected with the rotating piece in a matching mode, and the centrifugal piece is provided with a mounting position for limiting the reaction tube.
In one embodiment, the centrifugal piece comprises a connecting end and a free end which are oppositely arranged, the connecting end is rotatably installed on the rotating piece, the rotating axis of the centrifugal piece is intersected with the rotating axis of the rotating piece, and the free end is provided with a reaction tube installation cavity with two open ends to form the installation position.
In one embodiment, the centrifugal device comprises two centrifugal members, and the two centrifugal members are symmetrically arranged on two opposite sides of the rotating member by taking the rotating axis of the rotating member as a symmetry axis.
In one embodiment, the rotating member has a middle connecting portion and two retaining portions respectively located at two opposite ends of the middle connecting portion, the middle connecting portion is coupled to the driving assembly, each retaining portion includes two retaining arms spaced apart from each other, and the centrifugal member is limited between the two retaining arms and is rotatably connected to the two retaining arms.
In one embodiment, the centrifugal device further comprises a deflecting piece, two ends of the deflecting piece are respectively matched and connected with the holding arm and the centrifugal piece, and the centrifugal piece rotates relative to the holding arm by taking the deflecting piece as a rotation center.
In one embodiment, each of the clamping parts comprises a first clamping part and a second clamping part, the first clamping part is connected to one end of the middle connecting part, the second clamping part is arranged on one side of the first clamping part in a stacked mode, a first mounting groove is formed in one side, facing the second clamping part, of the first clamping part, a second mounting groove corresponding to the first mounting groove is formed in one side surface, facing the first clamping part, of the second clamping part, and the first mounting groove and the second mounting groove jointly form a mounting hole for limiting the rotating part.
In one embodiment, the centrifuge device further comprises a buffer assembly, and the drive assembly is supported on the mounting base by the buffer assembly.
In one embodiment, the centrifuge further comprises:
the positioning piece is arranged on the rotating piece and rotates along with the rotating piece; and
the sensing assembly is arranged on the mounting base and positioned on one side of the driving assembly, and the sensing assembly is used for detecting the position of the positioning piece;
when the sensing assembly detects that the positioning piece is located at the target position, the rotating piece is judged to be at the initial angle.
In one embodiment, the sensing assembly includes a signal transmitting unit and a signal receiving unit arranged at intervals, and when the positioning element is located between the signal transmitting unit and the signal receiving unit, it is determined that the rotating element is located at the target position.
According to an aspect of the present application, there is provided a nucleic acid detecting method using the above-described nucleic acid detecting apparatus, including the steps of:
mounting the reagent strip to a reagent strip mounting position;
extracting nucleic acid in a sample and transferring the nucleic acid into the reaction tube;
moving the reaction tube from the reagent strip to a centrifugal device, and centrifuging the sample in the reaction tube;
moving the reaction tube from the centrifugal device to a detection device, and carrying out amplification and fluorescence detection analysis on the sample in the reaction tube.
In the nucleic acid detecting apparatus and the nucleic acid detecting method, the sample in which the reaction in the reagent strip is completed can be transferred to the centrifugal device by the liquid transfer device, and after the centrifugation is completed by the centrifugal device, the sample is transferred to the detecting device by the liquid transfer device for detection. In the detection process, the reaction tube does not need to be manually taken out from the reagent strip and put into an additionally arranged centrifugal machine for centrifugation, so that manual operation is omitted, the detection efficiency of nucleic acid detection is improved, and the experiment cost is saved.
Drawings
FIG. 1 is a schematic structural view of a nucleic acid detecting apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic view showing the structure of a centrifuge of the nucleic acid detecting apparatus shown in FIG. 1.
The reference numbers illustrate:
100. a nucleic acid detection device; 10. installing a base; 12. mounting a bottom plate; 14. a first mounting base; 16. a second mounting base; 20. a reagent strip; 30. a centrifugal device; 31. a buffer assembly; 32. a drive assembly; 321. a drive support; 323. a drive member; 33. a rotating member; 332. an intermediate connecting portion; 334. a chucking section; 3341. a first retaining part; 3343. a second chucking part; 34. a centrifuge; 341. a connecting end; 343. a free end; 35. a deflection member; 36. a positioning member; 37. a sensing component; 372. mounting a bracket; 374. a signal transmitting unit; 376. a signal receiving unit; 40. a pipetting device; 41. a pipetting mechanism; 43. a liquid transferring gun; 50. a detection device; 200. and (3) a reaction tube.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
Referring to FIG. 1, FIG. 1 is a schematic view showing the structure of a nucleic acid detecting apparatus according to an embodiment of the present invention.
An embodiment of the present invention provides a nucleic acid detecting apparatus 100, which includes a mounting base 10, a reagent strip 20, a centrifugation device 30, a pipetting device 40, and a detecting device 50. Wherein, the reagent strip 20, the centrifugal device 30, the liquid-transferring device 40 and the detecting device 50 are all installed on the installation base 10, the reagent strip 20 is used for storing reagent, sample and the reaction tube 200, the centrifugal device 30 is used for centrifuging the sample in the reaction tube 200, the detecting device 50 is used for detecting the sample in the reaction tube 200, and the liquid-transferring device 40 is used for moving the reaction tube 200 among the reagent strip 20, the centrifugal device 30 and the detecting device 50.
In this manner, the nucleic acid detecting apparatus 100 is integrated with the centrifuge device 30, and the sample in which the reaction in the reagent strip 20 is completed can be transferred to the centrifuge device 30 by the pipette device 40, and after the centrifugation is completed by the centrifuge device 30, the sample is transferred to the detecting device 50 by the pipette device 40 to be detected. In the above detection process, the reaction tube 200 does not need to be manually taken out of the reagent strip 20 and put into an additionally arranged centrifuge for centrifugation, so that manual operation is omitted, the detection efficiency of nucleic acid detection is improved, and the experiment cost is saved.
Specifically, the mounting base 10 includes a mounting base plate 12, a first mounting base 14, and a second mounting base 16. In the following embodiments, the length direction of the mounting base plate 12 is a first direction (X direction shown in fig. 1), the width direction of the mounting base plate 12 is a second direction (Y direction shown in fig. 1), and the thickness direction of the mounting base plate 12 is a third direction (X direction shown in fig. 1). The first mounting base 14 is mounted on one side of the mounting baseplate 12 in the first direction, and the first mounting base 14 extends along the entire length in the first direction. The second mounting base 16 is mounted to the mounting baseplate 12 and located on one side of the first mounting base 14 in the second direction, the second mounting base 16 extending lengthwise in the second direction.
One side that first mounting base 14 kept away from second mounting base 16 in the first direction is equipped with reagent strip installation position, and reagent strip 20 detachably installs in reagent strip installation position, and stock solution storehouse, sample storehouse and holding bin have been seted up to reagent strip 20 in order to hold reagent, sample and reaction tube 200 respectively. Preferably, the reagent strip mounting location may be a mounting slot or a mounting track that mates with the reagent strip 30. It should be noted that, since the internal structure of the reagent strip 20 is not the main point of the present application, the detailed structure of the reagent strip 20 is not described herein.
The detection device 50 is mounted on the second mounting base 16, and the detection device 50 is used for amplifying and detecting fluorescence of the centrifuged sample to perform quantitative analysis on a specific DNA sequence. It should be noted that, since the internal structure of the detection device 50 does not belong to the main invention of the present application, the detailed structure of the detection device 50 is not described herein.
The pipetting device 40 includes a pipetting mechanism 41 and a pipetting gun 43, one end of the pipetting mechanism 41 is mounted on the mounting base plate 12 and located on the side of the second mounting base 16 away from the first mounting base 14, and the other end of the pipetting mechanism 41 extends in the first direction and is located above the reagent strip 20 and the detection device 50. The pipette gun 43 is movably mounted to the pipetting mechanism 41, and the pipette gun 43 is movable in a first direction above the reagent strip 20 and the detection device 50 to transfer the reaction tube 200. It should be noted that, since the internal structure of the pipetting device 40 does not belong to the main invention of the present application, the specific structure of the pipetting device 40 is not described herein.
Referring to fig. 2, fig. 2 is a schematic structural diagram of a centrifugal apparatus according to an embodiment of the present invention.
The centrifugal device 30 is mounted on one side of the first mounting base 14 close to the second mounting base 16 in the first direction, the centrifugal device 30 includes a buffer component 31, a driving component 32, a rotating component 33 and a centrifugal component 34, the driving component 32 is mounted on the first mounting base 14 through the buffer component 31, the reaction tube 200 can be limited in the centrifugal component 34, and the rotating component 33 drives the centrifugal component 34 to rotate under the driving of the driving component 32 so as to centrifuge the sample in the reaction tube 200.
Specifically, the buffering assembly 31 includes four buffering members, the four buffering members are arranged in an array on the first mounting base 14, and each buffering member can be deformed to play a buffering role in a recoverable manner under the action of external force, so that noise generated by vibration in the working process of the centrifugal device 30 is reduced. It can be understood that the number and the arrangement mode of the buffer parts are not limited, and the buffer parts can be arranged as required to achieve an ideal buffering and damping effect.
The driving assembly 32 includes a driving support 321 and a driving member 323. Drive mount 321 is supported on cushioning assembly 31 and is fixedly coupled to cushioning assembly 31 by fasteners. The driving member 323 is accommodated in the driving support 321, and an output shaft of the driving member 323 extends out of an end of the driving support 321 away from the mounting base to be coupled with the rotating member 33. Specifically, in one embodiment, the driving member 323 is a stepper motor for outputting torque. It will be appreciated that the particular type of drive member 323 is not limited and may be configured as desired to meet different drive requirements.
The rotating member 33 is coupled to an output shaft of the driving member 323, and the rotating member 33 is rotated about a rotation axis by the driving member 323. Specifically, the rotating member 33 includes a middle connecting portion 332 and two catches 334, the middle connecting portion 332 is fixed to the output shaft of the driving assembly 32 by fastening bolts, so that the rotating member 33 can rotate around a rotating shaft under the driving of the driving assembly 32. When the rotation member 33 rotates to the initial angle, the intermediate connection portion 332 extends lengthwise in the first direction. The two retainers 334 are respectively disposed at two opposite ends of the middle connecting portion 332 in the first direction, each retainer 334 includes two retaining arms spaced apart in the second direction, and each retaining arm is penetrated by a mounting hole extending in the second direction to mount the centrifugal piece 34.
Further, in an embodiment, each of the retainers 334 includes a first retainer 3341 and a second retainer 3343, the first retainer 3341 is integrally formed with the middle connecting portion 332, and the second retainer 3343 is stacked on a side of the first retainer 3341 facing away from the mounting base 10. The surface of one side, facing the second clamping part 3343, of the first clamping part 3341 is provided with a first mounting groove with a semicircular cross section, the surface of one side, facing the first clamping part 3341, of the second clamping part 3343 is provided with a second mounting groove with a semicircular cross section, and the second mounting groove and the first mounting groove are correspondingly communicated to form a mounting hole together.
In order to ensure the balance of the rotating member 33 during the rotation, the centrifugal device 30 includes two centrifugal members 34, the two centrifugal members 34 are respectively retained in the two retaining portions 334, so as to be symmetrically installed on two opposite sides of the rotating member 33 with the rotation axis of the rotating member 33 as a symmetry axis, and the reaction tube 200 can be inserted into any one of the centrifugal members 34.
The centrifugal member 34 is a block structure and is provided with a mounting position for limiting the reaction tube 200, the centrifugal member 34 is rotatably coupled to the rotating member 33, and the rotation axis of the centrifugal member 34 intersects with the rotation axis of the rotating member 33. Thus, when the driving member 323 drives the rotating member 33 to rotate, the centrifugal member 34 also deflects at a certain angle, so that the liquid in the reaction tube 200 fills the detection chamber at the bottom of the reaction tube 200 under the action of centrifugal force.
Specifically, in some embodiments, the eccentric 34 includes a connecting end 341 and a free end 343 that are disposed opposite to each other. The connecting end 341 of the centrifugal member 34 is rotatably mounted on the rotating member 33, and the free end 343 is provided with a reaction tube mounting cavity with two open ends to form a mounting position for limiting the reaction tube. In this way, the reaction tube 200 can be inserted into the reaction tube installation cavity by the liquid-transferring device 40, and when the rotating member 33 is driven by the driving member 323 to rotate, the centrifugal member 34 rotates along with the rotating member 33, and the free end 343 deflects outwardly to drive the reaction tube 200 to deflect outwardly.
More specifically, the centrifugal device 30 further includes a deflecting member 35, and both ends of the deflecting member 35 are respectively coupled to the chucking arm and the centrifugal member 34, and the centrifugal member 34 can be deflected with respect to the rotating member 33 with the deflecting member 35 as a rotation center. Specifically, in one embodiment, the deflecting member 35 is a sliding sleeve or a bearing, two rotating shafts are respectively protruded from two opposite ends of the connecting end 341 of the centrifugal member 34 in the second direction, one end of the deflecting member 35 is inserted into the mounting hole of the rotating member 33, and the rotating shaft of the centrifugal member 34 is inserted into the other end of the deflecting member 35. In this manner, the centrifugal member 34 is deflected with respect to the rotation member 33 by the deflection member 35 so that the sample can be concentrated on the bottom of the reaction tube 200.
In some embodiments, to control the rotation angle of the rotating member 33, the centrifugal device 30 further includes a positioning member 36 and a sensing assembly 37. The positioning member 36 is mounted to the rotating member 33 and rotates with the rotating member 33, and the sensing member 37 is spaced apart from one side of the driving member 32 to detect the position of the positioning member 36. When the sensing unit 37 detects that the positioning member 36 is located at the target position, it determines that the rotating member 33 is at the initial angle, and controls the driving unit 32 to stop operating so that the rotating member 33 stays at the initial angle, and the reaction tube 200 can be removed by the pipetting device 40.
The initial angle of the rotating member 33 is a position where the longitudinal direction of the rotating member 33 is parallel to the first direction, and when the rotating member 33 is at the initial angle, the two centrifugal members 34 are disposed at intervals in the first direction. It will be appreciated that the initial angle of the rotating member 33 can be adjusted by changing the mounting angle of the positioning member 36 relative to the rotating member 33 to meet different sampling requirements of the pipetting device 40.
Specifically, the positioning element 36 is a rod-shaped structure extending in a zigzag manner, one end of the positioning element 36 is mounted on one side of the middle connecting portion 332 of the rotating element 33, and the other end of the positioning element 36 is bent and extends in the second direction. The sensing assembly 37 includes a mounting bracket 372, a signal transmitting unit 374 and a signal receiving unit 376, the mounting bracket 372 is mounted on the mounting base plate 12, the signal transmitting unit 374 and the signal receiving unit 376 are mounted on the mounting bracket 372 and spaced apart from each other in the third direction, and the signal transmitting unit 374 can transmit signals to the signal receiving unit 376.
When the positioning element 36 is located at the target position, the positioning element 36 is located between the signal emitting unit 374 and the signal receiving unit 376, so as to block the signal emitted by the signal emitting unit 374, and the signal receiving unit 376 fails to receive the signal, so as to determine that the rotating element 33 is at the initial angle. Specifically, in an embodiment, the signal emitted by the signal emitting unit 374 is an optical signal, and the positioning element 36 can block the optical signal from reaching the signal receiving unit 376, so that the sensing element 37 can determine the rotation angle of the rotating member 33.
The present application also provides a nucleic acid detection method comprising the steps of:
s110: and installing the reagent strip 20 to the reagent strip installation site.
Specifically, the reagent strip 20 is mounted in a reagent strip mounting site on the first mounting base 14, and the reagent strip 20 accommodates the reaction tube 200 therein.
S120: the nucleic acid in the sample is extracted and transferred to the reaction tube 200.
S130: the reaction tube 200 is moved from the reagent strip 20 to the centrifuge device 30, and the sample in the reaction tube 200 is centrifuged.
Specifically, the reaction tube 200 is taken out from the reagent strip 20 by the pipetting device 40, and then the reaction tube 200 is moved into the centrifuge 34 of the centrifugation device 30, and the rotor 33 rotates the centrifuge 34 under the driving of the driving assembly 32 to centrifuge the sample containing nucleic acid in the reaction tube 200.
S140: the reaction tube 200 is moved from the centrifuge apparatus 30 to the detection apparatus 50, and the sample in the reaction tube 200 is subjected to amplification and fluorescence detection analysis.
Specifically, the reaction tube 200 is taken out from the centrifuge 34 by the pipetting device 40 into the detection device 50, and the detection device 50 performs amplification and fluorescence detection analysis on the sample containing nucleic acid in the reaction tube 200.
The nucleic acid detecting apparatus 100 integrates the reagent strip 10, the centrifugal device 30, the detecting device 50 and the liquid-moving device 40, the reaction tube 200 can be transferred to the centrifugal device 30 through the liquid-moving device 40 to automatically complete the centrifugation step, and then the reaction tube in the centrifugal device 30 is transferred to the detecting device 50 to be amplified and optically detected, without manually transferring the reaction tube 200 to an external centrifuge to be centrifuged, so that the operation convenience and the detection efficiency of nucleic acid detection are improved, the experiment cost is reduced, and the clinical requirements of the current increasingly developing are met.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (11)
1. A nucleic acid detecting apparatus characterized by comprising:
installing a base;
the reagent strip mounting position is arranged on the mounting base and is used for mounting reagent strips containing reaction tubes;
the centrifugal device is arranged on the mounting base and is used for centrifuging the sample in the reaction tube;
the detection device is arranged on the mounting base and is used for carrying out amplification and fluorescence detection analysis on the sample in the reaction tube; and
the liquid transferring device is arranged on the mounting base and used for moving the reaction tube.
2. The nucleic acid detecting apparatus according to claim 1, wherein the centrifugation means includes:
the driving assembly is arranged on the mounting base;
the rotating piece is connected to the driving assembly in a matching mode, and is driven by the driving assembly to rotate around a rotating axis; and
the centrifugal piece is rotatably connected with the rotating piece in a matching mode, and the centrifugal piece is provided with a mounting position for limiting the reaction tube.
3. The nucleic acid detecting apparatus according to claim 2, wherein the centrifuge includes a connecting end and a free end which are oppositely disposed, the connecting end is rotatably mounted to the rotating member, the rotation axis of the centrifuge intersects with the rotation axis of the rotating member, and the free end is provided with a reaction tube mounting cavity which is open at both ends to form the mounting position.
4. The nucleic acid detecting apparatus according to claim 2, wherein the centrifugal means includes two centrifugal members, and the two centrifugal members are mounted on opposite sides of the rotating member symmetrically with respect to the axis of rotation of the rotating member as an axis of symmetry.
5. The nucleic acid detecting apparatus according to claim 2, wherein the rotating member has a middle connecting portion and two holding portions respectively disposed at opposite ends of the middle connecting portion, the middle connecting portion is coupled to the driving assembly, each of the holding portions includes two holding arms spaced apart from each other, and the centrifugal member is disposed between and rotatably connected to the two holding arms.
6. The nucleic acid detecting apparatus according to claim 5, wherein the centrifuge further comprises a deflector having both ends respectively coupled to the chucking arm and the centrifuge, and the centrifuge rotates relative to the chucking arm with the deflector as a center of rotation.
7. The nucleic acid detecting apparatus according to claim 6, wherein each of the retainers includes a first retainer connected to one end of the intermediate connecting portion and a second retainer stacked on one side of the first retainer, a first mounting groove is formed in a side of the first retainer facing the second retainer, a second mounting groove is formed in a side surface of the second retainer facing the first mounting groove, the second mounting groove corresponds to the first mounting groove, and the first mounting groove and the second mounting groove together form a mounting hole for limiting the rotating member.
8. The nucleic acid detecting apparatus according to claim 2, wherein the centrifuge device further includes a buffer member, and the drive member is supported on the mounting base by the buffer member.
9. The nucleic acid detecting apparatus according to claim 2, wherein the centrifugation device further comprises:
the positioning piece is arranged on the rotating piece and rotates along with the rotating piece; and
the sensing assembly is arranged on the mounting base and positioned on one side of the driving assembly, and the sensing assembly is used for detecting the position of the positioning piece;
when the sensing assembly detects that the positioning piece is located at the target position, the rotating piece is judged to be at the initial angle.
10. The nucleic acid detecting apparatus according to claim 9, wherein the sensor unit includes a signal emitting unit and a signal receiving unit which are disposed at an interval, and when the positioning member is located between the signal emitting unit and the signal receiving unit, it is determined that the rotatable member is located at the target position.
11. A nucleic acid detecting method using the nucleic acid detecting apparatus according to any one of claims 1 to 10, comprising the steps of:
mounting the reagent strip to a reagent strip mounting position;
extracting nucleic acid in a sample and transferring the nucleic acid into the reaction tube;
moving the reaction tube from the reagent strip to a centrifugal device, and centrifuging the sample in the reaction tube;
moving the reaction tube from the centrifugal device to a detection device, and carrying out amplification and fluorescence detection analysis on the sample in the reaction tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110964695.5A CN113684120A (en) | 2021-08-20 | 2021-08-20 | Nucleic acid detection apparatus and nucleic acid detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110964695.5A CN113684120A (en) | 2021-08-20 | 2021-08-20 | Nucleic acid detection apparatus and nucleic acid detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
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