CN110437970A - Intestinal flora nucleic acid extraction system - Google Patents
Intestinal flora nucleic acid extraction system Download PDFInfo
- Publication number
- CN110437970A CN110437970A CN201910402228.6A CN201910402228A CN110437970A CN 110437970 A CN110437970 A CN 110437970A CN 201910402228 A CN201910402228 A CN 201910402228A CN 110437970 A CN110437970 A CN 110437970A
- Authority
- CN
- China
- Prior art keywords
- sample introduction
- centrifugation
- module
- sample
- test tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 29
- 238000000605 extraction Methods 0.000 title claims abstract description 21
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 20
- 238000005119 centrifugation Methods 0.000 claims abstract description 77
- 238000012360 testing method Methods 0.000 claims abstract description 68
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 58
- 239000000523 sample Substances 0.000 claims description 200
- 230000001360 synchronised effect Effects 0.000 claims description 24
- 238000012546 transfer Methods 0.000 claims description 14
- 238000005070 sampling Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 8
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 10
- 238000011160 research Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000000703 high-speed centrifugation Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Centrifugal Separators (AREA)
Abstract
Entitled intestinal flora nucleic acid extraction system of the invention.Belong to health care clinical testing techniques field.It mainly solves the problems, such as to use conventional manual method presence to take time and effort at present, working efficiency lowly, experimental result and quality cannot be guaranteed.Being mainly characterized by comprising: for it includes sample sample introduction module, intelligence centrifugation module, test tube sample introduction module, manipulator and reagent sample introduction module；Wherein, the intelligence centrifugation module is located in the middle part of machine bed plate；The sample sample introduction module and reagent sample introduction module are arranged in the middle layer frame of machine rack, positioned at the upper side of intelligence centrifugation module；The test tube sample introduction module is removably set in the middle layer frame of machine rack, positioned at the top of intelligence centrifugation module；The manipulator is arranged on the upper bed rack of machine rack.Present invention is mainly used for the bacterial nucleic acids automatically extracted in excrement.
Description
Technical field
The invention belongs to be mainly used in health care clinical testing techniques field more particularly to Automated inspection instrument
Stool sample intestinal flora nucleic acid extraction and the analysis automated instrument of flora, can expand other biological samples, as whole blood, blood plasma,
Nucleic acid extraction in the biological samples such as throat swab, tissue, sputum, cerebrospinal fluid.
Background technique
With the continuous development of modern biology technology, the research achievement of bioscience is widely used, and nucleic acid is
By many nucleotide polymerizations at large biological molecule compound, be life one of most basic substance, be organism hereditary information
Carrier, be widely present in all animal and plant cells, in microbial body, play important angle in the life process of biology
Color plays irreplaceable role in life section chief's research field.The primary step of nucleic acid extraction and purifying as nucleic acids research
Suddenly just seem increasingly important.
Intestinal microecology is referred to as the second largest genome of the mankind, and the research of intestinal flora is in medical field by pass in recent years
Note, intestinal flora nucleic acid extraction are mainly obtained by stool sample, currently used conventional manual method, and this method step is numerous
It is trivial, it takes time and effort, working efficiency is low, and experimental result and quality cannot be guaranteed.
Summary of the invention
There is provided a kind of intestinal flora nucleic acid extraction system the purpose of the present invention is to above-mentioned shortcoming, by from
Heart method, in addition being automatically performed the instrument for extracting bacterial nucleic acid from excrement using matched nucleic acid extracting reagent.
The technical solution of the invention is as follows: a kind of intestinal flora nucleic acid extraction system, it is characterised in that: including sample into
Egf block, intelligence centrifugation module, test tube sample introduction module, manipulator and reagent sample introduction module；Wherein, the intelligent centrifugal mold
Block is located in the middle part of machine bed plate；The sample sample introduction module and reagent sample introduction module are arranged in the middle layer frame of machine rack, position
In the upper side of intelligently centrifugation module；The test tube sample introduction module is removably set in the middle layer frame of machine rack, is located at intelligence
The top of module can be centrifuged；The manipulator is arranged on the upper bed rack of machine rack.
Sample sample introduction module described in technical solution of the invention includes sample sample introduction pallet and sample sample introduction electricity
Machine；Sample sample introduction motor and sample sample introduction synchronous belt are mounted in the middle layer frame of machine rack by sample sample introduction bracket, are located at
One upper side of intelligence centrifugation module；Sample sample introduction pallet is equipped on sample sample introduction bracket, and synchronous with sample sample introduction
Band connection.
Intelligence described in technical solution of the invention centrifugation module include centrifugal rotor positioning device, specimen holder and
It is centrifuged deep bid；Wherein, two opposite outsides of the specimen holder are equipped with longitudinal specimen holder fixing groove, and in addition opposite two sides are set
There is mechanical finger clamp fixing piece；The specimen holder is equipped with the fixation convex block cooperated with specimen holder fixing groove；The centrifugal rotor
Positioning device includes centrifugation shaft, centrifugation motor, centrifugal synchronous band, driven wheel and centrifugation locating piece, and centrifugation shaft passes through bearing
And bracket is mounted in the shell of intelligence centrifugation module, centrifugation motor joins through the driven wheel on centrifugal synchronous band and centrifugation shaft
It connects, centrifugation shaft lower end is coupled by centrifugation locating piece with centrifugation deep bid.
Test tube sample introduction module described in technical solution of the invention includes rack for test tube sample introduction pallet, centrifugation reagent adding
Disk and test tube sample introduction motor；Test tube sample introduction motor and test tube sample introduction synchronous belt are mounted on machine rack by test tube sample introduction bracket
In middle layer frame, positioned at the top of intelligence centrifugation module；Rack for test tube sample introduction pallet is equipped on test tube sample introduction bracket, and with examination
The connection of pipe sample introduction synchronous belt；The side of rack for test tube sample introduction pallet is arranged in centrifugation reagent adding disk.
Manipulator described in technical solution of the invention include can X/Y/Z to mobile manipulator and setting
Specimen holder transfer on the manipulator refers to folder and reagent adding needle tubing.
Reagent sample introduction module described in technical solution of the invention includes reagent sample introduction pallet and reagent sample introduction electricity
Machine；Reagent sample introduction motor and reagent sample introduction synchronous belt are mounted in the middle layer frame of machine rack by reagent sample introduction bracket, are located at
Above the other side of intelligence centrifugation module；Reagent sample introduction pallet is equipped on reagent sample introduction bracket, and same with reagent sample introduction
Step band connection.
It further include the disposable pipetting head device in conjunction with manipulator in technical solution of the invention；Disposably
Pipetting head device is made of sampling probe and disposable pipetting head.
Described in technical solution of the invention can X/Y/Z to mobile manipulator include the first lead screw motor of Z-direction,
The second lead screw motor of Z-direction, X are to motor, X to sliding block, Y-direction motor and Y-direction sliding block；X is loaded on machine rack to motor and X to sliding block
Upper bed rack on；Y-direction motor and Y-direction sliding block are loaded on X on sliding block；The first lead screw motor of Z-direction and the second lead screw motor of Z-direction are loaded on
On Y-direction sliding block；Specimen holder transfer, which refers to, to be clipped in the first lead screw motor of Z-direction；Reagent adding needle tubing is loaded on the second lead screw motor of Z-direction
On.
The center that deep bid is centrifuged described in technical solution of the invention is equipped with the installation cooperated with centrifugation locating piece
Hole, surrounding are symmetrically arranged with the installation slot position of four specimen holders；Specimen cup mounting groove there are four being set in the specimen holder.
The transfer of specimen holder described in technical solution of the invention refers to that folder includes two symmetrically arranged straight-bars, straight-bar
Lower end be equipped with inside kirby.
The invention has the following advantages that (1) sample sample introduction module: sample rack is included in the module of machine, sample to be processed
Box is placed in sample rack；(2) it is centrifuged module: having compared more than generic centrifuge rotor fixed position function, after sample is put into instrument, according to
Different samples carries out automatic centrifugation processing；(3) sample rack: being included in the module of machine by test tube sample introduction module, and test tube is placed on sample
In this；(4) manipulator: the corresponding work such as sample and reagent adding is collected in instrument for completing, (X/Y/Z can be achieved
To movement, X is moved left and right along gantry direction, and Y-direction is that front and back space is mobile, and Z-direction is to move up and down)；(5) reagent sample introduction mould
Block: required reagent is put into reagent sample introduction zone by regulation.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the structural schematic diagram of sample sample introduction module of the present invention.
Fig. 3 is the structural schematic diagram of present invention intelligence centrifugation module.
Fig. 4 is the schematic diagram of internal structure in Fig. 3.Centrifugation module runs at high speed and makes specimen centrifuge, and positioning is centrifuged after stopping
Part returns to zero under motor, driven wheel effect, judges sample number with this, carries out next step operation.
Fig. 5 is specimen holder of the present invention and centrifugation module partial enlarged view.
Fig. 6 is the structural schematic diagram of specimen cup of the present invention.
Fig. 7 is the structural schematic diagram of test tube sample introduction module of the present invention.
Fig. 8 is the structural schematic diagram of manipulator of the present invention.
Fig. 9 is that specimen holder of the present invention transfer refers to folder partial enlarged view.
Figure 10 is dynamic schematic diagram when specimen holder of the present invention transfer refers to folder transfer specimen holder.
Figure 11 is reagent sample introduction module structural schematic diagram of the present invention.
Figure 12 is the structural schematic diagram of the disposable pipetting head device part of the present invention.
In figure: 1. sample sample introduction modules；1-1. sample sample introduction pallet；1-2. sample sample introduction motor；2. intelligence centrifugation
Module；2-1. centrifugal rotor positioning device；2-2. specimen holder places position；2-3. specimen holder or rack for test tube；2-4. manipulator
Refer to folder fixing piece；2-5. specimen holder fixing groove；2-6. is centrifuged motor；2-7. centrifugal synchronous band；2-8. is centrifuged deep bid；2-9.
Driven wheel；2-10. being centrifuged locating piece；3. test tube sample introduction module；3-1. rack for test tube sample introduction pallet；3-2. is centrifuged reagent adding
Disk；4. manipulator；The transfer of 4-1. specimen holder refers to folder；4-2. reagent adding needle tubing；The first lead screw motor of 4-3. Z-direction；4-
4. Y-direction motor；The second lead screw motor of 4-5. Z-direction；4-6. X is to sliding block；4-7. X is to motor；4-8. Y-direction sliding block；5. examination
Agent sample introduction module；5-1. reagent sample introduction pallet；6. disposable pipetting head device；6-1. sampling probe；The disposable liquid relief of 6-2.
Head.
Specific embodiment
Description is described further to the specific embodiment of the invention with reference to the accompanying drawing.
As shown in Figures 1 to 12.Intestinal flora nucleic acid extraction system of the present invention includes sample sample introduction module 1, intelligence centrifugation
Module 2, test tube sample introduction module 3, manipulator 4 and reagent sample introduction module 5.Wherein, intelligence centrifugation module 2 is located at machine bed plate
Middle part.Sample sample introduction module 1 and reagent sample introduction module 5 are arranged in the middle layer frame of machine rack, positioned at intelligence centrifugation module 2
Above left side, right side.Test tube sample introduction module 3 is removably set in the middle layer frame of machine rack, is located at intelligence centrifugation module 2
Top.Manipulator 4 is arranged on the upper bed rack of machine rack.
Sample sample introduction module 1 includes sample sample introduction pallet 1-1 and sample sample introduction motor 1-2.Sample sample introduction motor 1-2 and sample
This sample introduction synchronous belt is mounted in the middle layer frame of machine rack by sample sample introduction bracket, positioned at the side of intelligence centrifugation module 2
Top.Sample sample introduction pallet 1-1 is equipped on sample sample introduction bracket, and is coupled with sample sample introduction synchronous belt.
Intelligence centrifugation module 2 includes centrifugal rotor positioning device 2-1, specimen holder 2-3 and centrifugation deep bid 2-8.Wherein, sample
Two opposite outsides of frame 2-3 are equipped with longitudinal specimen holder fixing groove 2-5, and in addition it is clamping fixed to be equipped with mechanical finger for opposite two sides
Part 2-4.Specimen holder 2-3 is equipped with the fixation convex block cooperated with specimen holder fixing groove 2-5.Centrifugal rotor positioning device 2-1 includes
It is centrifuged shaft, centrifugation motor 2-6, centrifugal synchronous band 2-7, driven wheel 2-9 and centrifugation locating piece 2-10, centrifugation shaft and passes through bearing
And bracket be mounted on intelligence centrifugation module 2 shell in, centrifugation motor 2-6 through centrifugal synchronous band 2-7 be centrifuged shaft on from
Driving wheel 2-9 connection, centrifugation shaft lower end are coupled by centrifugation locating piece 2-10 with centrifugation deep bid 2-8.It is centrifuged the center of deep bid 2-8
Equipped with the mounting hole with centrifugation locating piece 2-10 cooperation, surrounding is symmetrically arranged with the installation slot position of four specimen holder 2-3.Specimen holder 2-
Specimen cup mounting groove there are four being set in 3.
Test tube sample introduction module 3 includes rack for test tube sample introduction pallet 3-1, centrifugation reagent adding disk 3-2 and test tube sample introduction motor.Test tube
Sample introduction motor and test tube sample introduction synchronous belt are mounted in the middle layer frame of machine rack by test tube sample introduction bracket, are located at intelligence centrifugation
The top of module 2.Rack for test tube sample introduction pallet 3-1 is equipped on test tube sample introduction bracket, and is coupled with test tube sample introduction synchronous belt.
The side of rack for test tube sample introduction pallet 3-1 is arranged in centrifugation reagent adding disk 3-2.
The sample sample introduction bracket of sample sample introduction module 1 and the test tube sample introduction of sample sample introduction pallet 1-1 and test tube sample introduction module 3
Bracket and rack for test tube sample introduction pallet 3-1 appearance are identical.Specimen holder 2-3 can be rack for test tube.What is put in sample sample introduction module 1 is to be processed
Sample, the test tube that test tube sample introduction module 3 is put.
Manipulator 4 include can X/Y/Z to mobile manipulator and the specimen holder being arranged on the manipulator transfer refer to
Press from both sides 4-1 and reagent adding needle tubing 4-2.Can X/Y/Z to mobile manipulator include the first lead screw motor of Z-direction 4-3, the second screw rod of Z-direction
Motor 4-5, X are to motor 4-7, X to sliding block 4-6, Y-direction motor 4-4 and Y-direction sliding block 4-8.X is filled to motor 4-7 and X to sliding block 4-6
In on the upper bed rack of machine rack.Y-direction motor 4-4 and Y-direction sliding block 4-8 is loaded on X on sliding block 4-6.The first lead screw motor of Z-direction 4-
3 and Z-direction the second lead screw motor 4-5 is loaded on Y-direction sliding block 4-8.Specimen holder transfer refers to folder 4-1 loaded on the first lead screw motor of Z-direction 4-3
On.Reagent adding needle tubing 4-2 is loaded on the second lead screw motor of Z-direction 4-5.Specimen holder transfer refers to that folder 4-1 includes two symmetrically arranged
The lower end of straight-bar, straight-bar is equipped with inside kirby.Manipulator 4 is combined with disposable pipetting head device 6.It is disposable to move
Liquid head device 6 is made of sampling probe 6-1 and disposable pipetting head 6-2.
Reagent sample introduction module 5 includes reagent sample introduction pallet 5-1 and reagent sample introduction motor.Reagent sample introduction motor and reagent sample introduction
Synchronous belt is mounted in the middle layer frame of machine rack by reagent sample introduction bracket, above the other side of intelligence centrifugation module 2.
Reagent sample introduction pallet 5-1 is equipped on reagent sample introduction bracket, and is coupled with reagent sample introduction synchronous belt.
Workflow:
1, test tube is put into specimen holder 2-3, specimen holder 2-3 is included in instrument by sample sample introduction pallet 1-1；Sample is put, into mark
This；
2, refer to that folder 4-1 in conjunction with mechanical finger folder fixing piece 2-4 (as shown in Figure 10), drives specimen holder transfer by specimen holder transfer
It is put into centrifugation module specimen holder and places position 2-2, specimen holder places position 2-2 and specimen holder fixing groove 2-5 and matches combination；Manipulator
Finger is pressed from both sides in conjunction with specimen holder, is operated, specimen holder and centrifugal mold agllutination close；
3, under program, using centrifuge low speed rotation by specimen centrifuge (and the common centrifugation on the market in specimen holder 2-3
Machine is the same), after centrifugation is completed, fixed direction or position, rotor are gone to by module is centrifuged by centrifugal rotor positioning device 2-1
After fixation, specimen holder is taken out by manipulator and puts back to sample sample introduction module origin-location；First step centrifugal treating (mesh
Be reject stool sample in impurity, by the first step be centrifuged impurity sink to it is following)；
4, for manipulator in conjunction with disposable pipetting head 6-2, sampling probe 6-1 takes out (suction) supernatant, is then injected into test tube sample introduction mould
In test tube in block 3, it is to be painted to throw away disposable pipetting head 6-2(discard bin)；Sample needed for shifting；
5, the rack for test tube in test tube sample introduction module 3 is put into centrifugation module and carries out high speed centrifugation (revolving speed difference, behaviour by manipulator
Make step with centrifugation step above), then rack for test tube is taken out and puts back to 3 origin-location of test tube sample introduction module；Second step centrifugation
It handles (sample of purification, concentrate visible component more)；
6, manipulator is taken out the related disposable pipetting head 6-2 of (suction) supernatant and is thrown away together into discarded in conjunction with disposable pipetting head 6-2
Case (to be painted), remaining precipitating about 1ml in test tube；
7, manipulator sampling probe 6-1(effect is addition lysate) from about 20ml core is drawn in reagent sample introduction module 5 in reagent bottle
Lysate required for acid extracts, is filled into previous step test tube and (mixes with precipitating remaining in test tube about 1ml)；Addition cracking
Liquid；
8, the rack for test tube in test tube sample introduction module 3 is put into centrifugation module and carries out high speed centrifugation about 30min(revolving speed by manipulator
Difference, operating procedure is with centrifugation step above), after rotor is fixed, then rack for test tube is taken out and puts back to test tube sample introduction module 3
Origin-location；Third step centrifugal treating (cracking destroys cell wall and cytoplasm, obtains required nucleic acid)；
9. manipulator is in conjunction with disposable pipetting head, take out the related disposable pipetting head of (suction) supernatant throw away together into discard bin (to
Draw), leave about 0.2g precipitating (the macro basic group of nucleic acid of the fecal bacteria as extracted)；
10. completed sample sample introduction module, test tube sample introduction module, reagent sample introduction module automatically exit from instrument, test tube sample introduction
What test tube was collected is the nucleic acid extraction liquid that we need in module 3；Collect nucleic acid.
Manipulator disposable pipetting head in conjunction with disposable pipetting head avoids cross contamination.
Claims (10)
1. a kind of intestinal flora nucleic acid extraction system, it is characterised in that: including sample sample introduction module (1), intelligence centrifugation module
(2), test tube sample introduction module (3), manipulator (4) and reagent sample introduction module (5)；Wherein, intelligence centrifugation module (2) position
In the middle part of machine bed plate；The sample sample introduction module (1) and reagent sample introduction module (5) are arranged in the middle layer frame of machine rack,
Positioned at the upper side of intelligence centrifugation module (2)；The test tube sample introduction module (3) is removably set on the middle layer frame of machine rack
On, it is located at the top of intelligence centrifugation module (2)；The manipulator (4) is arranged on the upper bed rack of machine rack.
2. a kind of intestinal flora nucleic acid extraction system according to claim 1, it is characterised in that: the sample sample introduction mould
Block (1) includes sample sample introduction pallet (1-1) and sample sample introduction motor (1-2)；Sample sample introduction motor (1-2) and sample sample introduction are synchronous
Band is mounted in the middle layer frame of machine rack by sample sample introduction bracket, is located at a upper side of intelligence centrifugation module (2)；Sample
Sample introduction pallet (1-1) is equipped on sample sample introduction bracket, and is coupled with sample sample introduction synchronous belt.
3. a kind of a kind of intestinal flora nucleic acid extraction system according to claim 1 or 2, it is characterised in that: the intelligence
It includes centrifugal rotor positioning device (2-1), specimen holder (2-3) and centrifugation deep bid (2-8) that module (2), which can be centrifuged,；Wherein, the mark
This (2-3) two opposite outsides are equipped with longitudinal specimen holder fixing groove (2-5), and in addition opposite two sides are equipped with mechanical finger
It presss from both sides fixing piece (2-4)；The specimen holder (2-3) is equipped with the fixation convex block cooperated with specimen holder fixing groove (2-5)；The centrifugation
Rotor fixed position device (2-1) includes centrifugation shaft, centrifugation motor (2-6), centrifugal synchronous band (2-7), driven wheel (2-9) and centrifugation
Locating piece (2-10), centrifugation shaft are mounted in the shell of intelligence centrifugation module (2) by bearing and bracket, and motor (2- is centrifuged
6) couple through centrifugal synchronous band (2-7) with the driven wheel (2-9) in centrifugation shaft, centrifugation shaft lower end passes through centrifugation locating piece
(2-10) couples with centrifugation deep bid (2-8).
4. a kind of intestinal flora nucleic acid extraction system according to claim 1 or 2, it is characterised in that: the test tube into
Egf block (3) includes rack for test tube sample introduction pallet (3-1), centrifugation reagent adding disk (3-2) and test tube sample introduction motor；Test tube sample introduction motor
And test tube sample introduction synchronous belt is mounted in the middle layer frame of machine rack by test tube sample introduction bracket, is located at intelligence centrifugation module (2)
Top；Rack for test tube sample introduction pallet (3-1) is equipped on test tube sample introduction bracket, and is coupled with test tube sample introduction synchronous belt；From
Heart reagent adding disk (3-2) is arranged in the side of rack for test tube sample introduction pallet (3-1).
5. a kind of intestinal flora nucleic acid extraction system according to claim 1 or 2, it is characterised in that: the manipulator
Module (4) include can X/Y/Z to mobile manipulator and the specimen holder being arranged on the manipulator transfer refer to folder (4-1) and add an examination of
Agent needle tubing (4-2).
6. a kind of intestinal flora nucleic acid extraction system according to claim 1 or 2, it is characterised in that: the reagent into
Egf block (5) includes reagent sample introduction pallet (5-1) and reagent sample introduction motor；Reagent sample introduction motor and reagent sample introduction synchronous belt pass through
Reagent sample introduction bracket is mounted in the middle layer frame of machine rack, is located above the other side of intelligence centrifugation module (2)；Reagent sample introduction
Pallet (5-1) is equipped on reagent sample introduction bracket, and is coupled with reagent sample introduction synchronous belt.
7. a kind of intestinal flora nucleic acid extraction system according to claim 1 or 2, it is characterised in that: further include and machinery
The disposable pipetting head device (6) that fingerprint block (4) combines；Disposable pipetting head device (6) is by sampling probe (6-1) and disposable shifting
Liquid head (6-2) is constituted.
8. a kind of intestinal flora nucleic acid extraction system according to claim 5, it is characterised in that: it is described can X/Y/Z to
Mobile manipulator includes the first lead screw motor of Z-direction (4-3), the second lead screw motor of Z-direction (4-5), X to motor (4-7), X to cunning
Block (4-6), Y-direction motor (4-4) and Y-direction sliding block (4-8)；X is to motor (4-7) and X to sliding block (4-6) loaded on the upper of machine rack
In layer frame；Y-direction motor (4-4) and Y-direction sliding block (4-8) are loaded on X on sliding block (4-6)；The first lead screw motor of Z-direction (4-3) and Z-direction
Second lead screw motor (4-5) is loaded on Y-direction sliding block (4-8)；Specimen holder transfer refers to folder (4-1) loaded on the first lead screw motor of Z-direction (4-
3) on；Reagent adding needle tubing (4-2) is loaded in the second lead screw motor of Z-direction (4-5).
9. a kind of intestinal flora nucleic acid extraction system according to claim 3, it is characterised in that: the centrifugation deep bid
The center of (2-8) is equipped with the mounting hole with centrifugation locating piece (2-10) cooperation, and surrounding is symmetrically arranged with the peace of four specimen holders (2-3)
Tankage position；Specimen cup mounting groove there are four being set in the specimen holder (2-3).
10. a kind of intestinal flora nucleic acid extraction system according to claim 5, it is characterised in that: the specimen holder turns
Shifting refers to that folder (4-1) includes two symmetrically arranged straight-bars, and the lower end of straight-bar is equipped with inside kirby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910402228.6A CN110437970A (en) | 2019-05-15 | 2019-05-15 | Intestinal flora nucleic acid extraction system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910402228.6A CN110437970A (en) | 2019-05-15 | 2019-05-15 | Intestinal flora nucleic acid extraction system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110437970A true CN110437970A (en) | 2019-11-12 |
Family
ID=68428818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910402228.6A Pending CN110437970A (en) | 2019-05-15 | 2019-05-15 | Intestinal flora nucleic acid extraction system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110437970A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111394220A (en) * | 2020-03-24 | 2020-07-10 | 北京万泰生物药业股份有限公司 | Nucleic acid extraction device |
| CN111481235A (en) * | 2020-03-05 | 2020-08-04 | 深圳市人民医院 | Intelligent throat swab specimen collecting robot |
| CN112630453A (en) * | 2020-12-23 | 2021-04-09 | 贵州金域医学检验中心有限公司 | Constant temperature kit for gynecological tumor marker detection and detection method thereof |
| CN113684120A (en) * | 2021-08-20 | 2021-11-23 | 圣湘生物科技股份有限公司 | Nucleic acid detection apparatus and nucleic acid detection method |
| CN114196516A (en) * | 2020-09-02 | 2022-03-18 | 浙江康是医疗器械有限公司 | Full-automatic portable virus sampling pipe rapid analysis equipment |
| CN114437925A (en) * | 2022-03-15 | 2022-05-06 | 成都瀚辰光翼科技有限责任公司 | Nucleic acid extractor and control method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0569115A2 (en) * | 1992-05-05 | 1993-11-10 | General Atomics | High throughput DNA preparation system |
| CN103399162A (en) * | 2002-05-17 | 2013-11-20 | 贝克顿·迪金森公司 | Automated system for isolating, amplifying and detecting target nucleic acid sequence |
| CN104407160A (en) * | 2014-10-29 | 2015-03-11 | 东软安德医疗科技有限公司 | Fully automatic centrifuge apparatus |
| CN108588066A (en) * | 2018-05-08 | 2018-09-28 | 广州高盛智造科技有限公司 | A kind of equipment and its control method automatically extracting nucleic acid |
| CN210856068U (en) * | 2019-05-15 | 2020-06-26 | 湖北微伞医疗科技有限公司 | Intestinal flora nucleic acid extraction system |
-
2019
- 2019-05-15 CN CN201910402228.6A patent/CN110437970A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0569115A2 (en) * | 1992-05-05 | 1993-11-10 | General Atomics | High throughput DNA preparation system |
| CN103399162A (en) * | 2002-05-17 | 2013-11-20 | 贝克顿·迪金森公司 | Automated system for isolating, amplifying and detecting target nucleic acid sequence |
| CN104407160A (en) * | 2014-10-29 | 2015-03-11 | 东软安德医疗科技有限公司 | Fully automatic centrifuge apparatus |
| CN108588066A (en) * | 2018-05-08 | 2018-09-28 | 广州高盛智造科技有限公司 | A kind of equipment and its control method automatically extracting nucleic acid |
| CN210856068U (en) * | 2019-05-15 | 2020-06-26 | 湖北微伞医疗科技有限公司 | Intestinal flora nucleic acid extraction system |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111481235A (en) * | 2020-03-05 | 2020-08-04 | 深圳市人民医院 | Intelligent throat swab specimen collecting robot |
| CN111481235B (en) * | 2020-03-05 | 2021-03-16 | 深圳市人民医院 | Intelligent throat swab specimen collecting robot |
| CN111394220A (en) * | 2020-03-24 | 2020-07-10 | 北京万泰生物药业股份有限公司 | Nucleic acid extraction device |
| CN111394220B (en) * | 2020-03-24 | 2023-05-02 | 北京康彻思坦生物技术有限公司 | Nucleic acid extraction device |
| CN114196516A (en) * | 2020-09-02 | 2022-03-18 | 浙江康是医疗器械有限公司 | Full-automatic portable virus sampling pipe rapid analysis equipment |
| CN112630453A (en) * | 2020-12-23 | 2021-04-09 | 贵州金域医学检验中心有限公司 | Constant temperature kit for gynecological tumor marker detection and detection method thereof |
| CN113684120A (en) * | 2021-08-20 | 2021-11-23 | 圣湘生物科技股份有限公司 | Nucleic acid detection apparatus and nucleic acid detection method |
| CN114437925A (en) * | 2022-03-15 | 2022-05-06 | 成都瀚辰光翼科技有限责任公司 | Nucleic acid extractor and control method thereof |
| CN114437925B (en) * | 2022-03-15 | 2024-02-27 | 成都瀚辰光翼科技有限责任公司 | Nucleic acid extraction instrument and control method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110437970A (en) | Intestinal flora nucleic acid extraction system | |
| CN201152848Y (en) | Bionic intelligent type blood rheogeniometer | |
| CN108048302A (en) | Full automatic instrument for extracting nucleic acid | |
| CN108949505A (en) | A kind of nucleic acid-extracting apparatus and method | |
| CN111239128A (en) | Vaginal secretion detector and detection method thereof | |
| CN107760676B (en) | Full-automatic extraction instrument for paramagnetic particles | |
| CN111704996A (en) | Intelligent full-automatic biosafety pathogenic microorganism research system | |
| CN202735146U (en) | Coloration device | |
| CN110819623A (en) | Nucleic acid extraction instrument and extraction method | |
| CN108195654A (en) | A kind of automatic smear overflow dyeing machine | |
| CN117385000A (en) | Multitasking parallel nucleic acid extraction and transformation method and device thereof | |
| CN208568812U (en) | A kind of Biochemical Analyzer | |
| CN210856068U (en) | Intestinal flora nucleic acid extraction system | |
| CN102243243A (en) | Novel instrument for automatically processing phlegm sample | |
| CN210968932U (en) | Sampler clamping device | |
| KR102298299B1 (en) | Integrated cell separation device capable of centrifugation and magnetic separation | |
| CN115746999A (en) | Nucleic acid extraction instrument | |
| CN114088958A (en) | Full-automatic platelet antibody detection analyzer and use method thereof | |
| CN212864734U (en) | Automatic nucleic acid extraction instrument | |
| CN112034157A (en) | Biochemical immunodetection equipment of reproductive medicine | |
| CN212879380U (en) | Pathological specimen collector | |
| CN110408522B (en) | Automatic sampling and sample adding system for blood culture | |
| CN216718460U (en) | Full-automatic platelet antibody detection analyzer | |
| CN218901620U (en) | Blood sample shakes even device | |
| CN216192225U (en) | Nucleic acid extraction instrument for laboratory detection of batch sample detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191112 |