CN114088958A - Full-automatic platelet antibody detection analyzer and use method thereof - Google Patents

Abstract

The invention relates to a full-automatic platelet antibody detection analyzer and a using method thereof, wherein the analyzer comprises a control system, a circuit board, a base, a suction head storage module, a sample storage module, a gel card storage puncture module, a test waste discarding module, a centrifugal incubation module, a reagent storage module, a card clamping manipulator module, a detection module, a beam module and a sample adding arm slide rail module, and the using method comprises the following steps: placing a suction head, a platelet suspension, a diluent, a fluorescent reagent, a sample tube and a gel card into corresponding positions, and setting detection sample parameters; puncturing the sealing film of the gel card; adding a platelet suspension, a diluent, a fluorescent reagent and a plasma sample into the gel card; after the gel card is incubated and centrifuged, photographing and interpretation and fluorescence intensity detection are carried out, two qualitative detection results and quantitative detection results are given, the gel card is discarded after the detection is finished, and the steps are repeated until all the detection is finished.

Description

Full-automatic platelet antibody detection analyzer and use method thereof

Technical Field

The invention relates to the field of medical instruments, in particular to a full-automatic platelet antibody detection analyzer and a using method thereof.

Background

Platelet antigens can stimulate the body to produce platelet antibodies by various means such as blood transfusion, pregnancy, organ transplantation and the like, so as to cause platelet destruction, and cause immune thrombocytopenia, such as platelet transfusion ineffectiveness (PTR), fetal allogeneic immune thrombocytopenia (FMAIT), post-transfusion purpura (PTP) and the like. In particular, in recent years, platelet transfusion has been widely used for the treatment of clinical thrombocytopenia patients, such as autoimmune thrombocytopenia, neonatal thrombocytopenia, aplastic anemia, tumors, and the like. Currently, there is no fully automatic platelet antibody detection analyzer in the market, but the platelet antibody detection methods commonly used in clinic usually need manual operation and interpretation, and the detection methods usually include the MAIPA method and the flow cytometry method, wherein the MAIPA method is a manual operation test, and platelets need to be lysed in the test process, and the method has complicated operation steps and long time (more than 5 hours), and is difficult to meet the requirements of routine clinical detection. The flow cytometry method needs expensive flow cytometry equipment, needs repeated washing in the detection process, consumes time and labor, has low detection flux and cannot meet the clinical detection requirement. At present, an effective and practical platelet antibody detection technology is still lacking clinically, and platelet infusion ineffective events caused by platelet antibodies occur frequently, so that not only is the treatment of patients delayed, but also precious platelet resources are wasted.

Disclosure of Invention

The invention aims to provide a full-automatic platelet antibody detection analyzer, which is beneficial to clinical diagnosis of platelet immune diseases, can also carry out blood type compatibility detection before platelet infusion and greatly improves the infusion curative effect.

In order to achieve the above purpose, the following technical scheme is provided:

the analyzer comprises a control system, a circuit board, a base, a sucker storage module, a sample storage module, a gel card storage puncture module, a test waste discarding module, a centrifugal incubation module, a reagent storage module, a clamping manipulator module, a detection module, a beam module and a sample adding arm slide rail module, wherein the control system controls the circuit board to operate, the circuit board controls the action of each module, the sucker storage module, the sample storage module, the gel card storage puncture module, the test waste discarding module, the centrifugal incubation module, the reagent storage module, the clamping manipulator module, the detection module and the beam module are all fixedly connected to the base, and the sucker storage module, the sample storage module, the gel card storage puncture module, the centrifugal incubation module, the reagent storage module, the clamping manipulator module, the detection module and the beam module are sequentially arranged from left to right along the length direction of the base, The test waste abandons module and centrifugal incubation module, reagent storage module is located suction head storage module rear side, the circuit board is located reagent storage module rear side, it abandons the module rear side to press from both sides card manipulator module to be located test waste, it is located centrifugal incubation module rear side to detect the module, the crossbeam module includes sliding support and synchronous support, sliding support and synchronous support's length direction is parallel with base length direction, and the symmetry sets up both ends around the base respectively, the front end sliding connection of application of sample arm slide rail module is on sliding support, and its rear end is installed on synchronous support.

Preferably, the sucker storage module is of a drawer-type push-pull structure, two sucker boxes are arranged in front of and behind the sucker storage module side by side, and a certain number of suckers are arranged in the sucker boxes;

the sample storage module is provided with six rows of sample racks side by side on the left and right, each row of sample racks can contain twelve sample tubes, and each row of sample racks is provided with an independent slide way in a matching way;

the gel card storage puncturing module comprises a gel card clamping seat, a track, a screw rod, a clamping motor, a sliding block and a puncturing mechanism, wherein a certain number of gel card clamping positions are arranged in parallel in the front and at the back of the top of the gel card clamping seat, the bottom of the gel card clamping seat is fixedly connected with the sliding block, the bottom surface of the sliding block is abutted against the top surface of the track and can slide relatively, the clamping motor is fixedly connected to one end of the track, the output end of the clamping motor is fixedly connected with one end of the screw rod, the screw rod penetrates through the sliding block and is in threaded connection with the sliding block, the other end of the screw rod is rotatably connected with the other end of the track, the puncturing mechanism is positioned above the middle of the track and comprises a puncturing bracket, a puncturing motor, an eccentric wheel, a puncturing slide rail, a puncturing sliding block, a puncturing spring and a knife holder, the bottom end of the puncturing bracket is fixedly connected to the base, the puncturing motor is fixedly connected to the upper part of the puncturing bracket, and the output end of the eccentric wheel is fixedly connected to the puncturing motor, the number of the puncture slide rails is two, the puncture slide rails are respectively and symmetrically fixedly connected to the middle of the puncture bracket, each puncture slide rail is connected with one puncture slide block in a sliding manner, the tool apron is fixedly connected with the two puncture slide blocks, a certain number of puncture knives are arranged on the tool apron, two tension springs are arranged in front of the two puncture slide rails respectively, one end of each tension spring is fixedly connected to the puncture bracket, and the other end of each tension spring is fixedly connected to the tool apron;

the test waste discarding module is of a drawer type push-pull structure, and three pairs of light pipes are arranged on two sides of the test waste discarding module so as to monitor whether the test waste discarding module is filled;

the centrifugal incubation module comprises a centrifuge and a temperature control module, the temperature control module comprises a heating belt and a temperature sensor, the heating belt is arranged on the bottom surface of the centrifuge, and the temperature sensor is arranged at the top of the side surface of the centrifuge;

the reagent storage module is provided with three reagent positions on the left and right side in parallel;

the clamping manipulator module is sequentially provided with a clamping module mounting seat, a manipulator telescopic component, a manipulator mounting seat, a finger component, a manipulator rotating component and a rubber sleeve from bottom to top, the manipulator telescopic component comprises a telescopic belt, a telescopic motor, a fourth pulley block and a lifting block, one side of the telescopic motor is horizontally and fixedly connected to the bottom outside the clamping module mounting seat, the output end of the telescopic motor penetrates through the clamping module mounting seat, the fourth pulley block comprises a fourth driving pulley and a fourth driven pulley, the fourth driving pulley is fixedly connected to the output end of the telescopic motor, the fourth driven pulley is mounted at the top end of the inner side of the clamping module mounting seat, the inner side of the telescopic belt is connected with the fourth pulley block in a matching manner, the lifting block is fixedly connected with one side of the telescopic belt, and the manipulator rotating component comprises a rotary rod, a rotary belt, a rubber sleeve, The manipulator comprises a rotating motor and a third pulley block, wherein one side of the rotating motor is vertically and fixedly connected to the upper part of a clamping module mounting seat, the output end of the rotating motor penetrates through the clamping module mounting seat, two ends of a rotating rod are respectively and rotatably connected with the clamping module mounting seat, the third driven pulley block comprises a third driving pulley and a third driven pulley, the third driving pulley is fixedly connected to the output end of the rotating motor, a third driven pulley is fixedly connected to the top end of the rotating rod, a rubber sleeve is fixedly sleeved in one end of the manipulator mounting seat, a finger component is fixedly connected to the top surface of the other end of the manipulator mounting seat, and the rotating rod penetrates through the rubber sleeve and is in sliding friction connection with the rubber sleeve;

the detection module comprises a fixed plate, a constant current source, a detection module mounting frame, a light source, an optical detection module, an optical darkroom, a camera fixing seat, a light source sliding rail and a backlight source, wherein the constant current source, the detection module mounting frame, the camera fixing seat, the light source sliding rail and the backlight source are all fixedly connected to the fixed plate;

the synchronous support is further provided with a synchronous belt, a first pulley block, a synchronous motor, a synchronous slide rail and a sliding table, the bottom end of the synchronous motor is fixedly connected to the upper portion of the right end of the synchronous support, the first pulley block comprises a first driving pulley and a first driven pulley, the first driving pulley is fixedly connected with the output end of the synchronous motor, the first driven pulley is mounted at the top end of the left side of the synchronous support, the inner side of the synchronous belt is connected with the first pulley block in a matched mode, the synchronous slide rail is arranged on the rear side of the synchronous belt, the sliding table is connected with the synchronous slide rail in a sliding mode, and one side of the sliding table is fixedly connected with the synchronous belt;

the sample adding arm slide rail module comprises a sample adding arm slide rail and three electronic liquid-transfering guns arranged side by side from front to back, the bottom of the front end of the sample adding arm slide rail is connected to the sliding support in a sliding way, the bottom of the back end of the sample adding arm slide rail is fixedly connected with the top surface of the sliding table, and the upper part of the inner side of the sample adding arm slide rail is also provided with three groups of transmission mechanisms arranged side by side from top to bottom, taking one group as an example, each transmission mechanism comprises a second pulley block, a slide plate, a transmission motor and a transmission belt, the second pulley block comprises a second driving pulley and a second driven pulley, the second driven pulley is arranged at the front end of the inner side of the sample adding arm slide rail, one side of the transmission motor is fixedly connected with the rear end of the inner side of the sample adding arm slide rail, the inner side of the transmission belt is connected with the second pulley block in a matching way, one side of the slide plate is connected with the sample adding arm slide rail in a sliding way, and penetrates through the transmission belt and is fixedly connected with one side of the transmission belt, the other side of the sliding plate is fixedly connected with the electronic liquid-transfering gun.

Preferably, the method of use comprises the steps of:

(1) before detection, an operator puts a suction head into a suction head box (the system automatically records the balance of the suction head detected last time), puts platelet suspension, diluent and fluorescent reagent into three reagent positions of a reagent storage module from left to right in sequence, puts sample tubes into a sample rack, and puts a corresponding number of gel cards (six gel cards per gel card) into a gel card storage area according to the number of the sample tubes to be detected;

(2) setting parameters such as the number of detected samples, incubation temperature, incubation time, centrifugation speed, centrifugation time and the like (the parameters can be named and stored) by using a control system;

(3) the control system carries out the following operation instructions according to the number of samples:

firstly, transferring the gel card to a gel card storage puncturing module to puncture a sealing film;

sequentially adding platelet suspension, diluent, fluorescent reagent and a patient plasma sample into the gel card by using the sample adding arm sliding rail module;

grabbing the gel card from the gel card storage area by using a clamping manipulator module, and putting the gel card into a centrifugal incubation module; repeating the clamping action, and putting all the gel cards added with the samples into a centrifugal incubation module;

fourthly, placing the gel card into the detection module by using the clamping manipulator module after incubation and centrifugation at constant temperature;

fifthly, after entering the detection module, the gel card is photographed and interpreted, then is transmitted to an optical darkroom through a light source slide rail to be detected in fluorescence intensity, and qualitative and quantitative detection results are given through an image processing algorithm and an A/D amplification algorithm;

sixthly, after the detection of one gel card is finished, the clamping manipulator module is used for grabbing the gel card, the gel card is rotated to the test waste discarding module to be discarded, the clamping manipulator module is used for grabbing the gel card from the centrifugal incubation module and placing the gel card into the detection module for detection, and the steps are repeated until all detection is finished.

The invention has the beneficial effects that:

the invention is used together with the gel card, can replace manual operation, automatically complete a series of processes such as sample adding, incubation, centrifugation, photographing interpretation, fluorescence intensity analysis and the like, can detect platelet antibodies and carry out blood type compatibility detection before platelet infusion, reduces the occurrence of ineffective events of platelet infusion, and is beneficial to clinically diagnosing platelet immune diseases and improving the curative effect of platelet infusion.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a tip storage module;

FIG. 3 is a schematic structural diagram of a sample memory module;

FIG. 4 is a schematic structural view of a gel card storage puncturing module;

FIG. 5 is a schematic structural view of a clamping robot module;

FIG. 6 is a schematic structural diagram of a detection module;

FIG. 7 is a schematic structural diagram of a beam module;

FIG. 8 is a top view of FIG. 7;

the reference symbols shown in the figures are: 1-tip storage module, 2-sample holder, 3-gel card holder, 4-test waste disposal module, 5-centrifuge, 6-reagent site, 7-puncture knife, 8-telescopic belt, 9-optical darkroom, 10-crossbeam module, 11-sample arm slide rail, 12-electronic pipetting gun, 13-rotating belt, 14-clip module mount, 15-finger part, 16-manipulator mount, 17-fixing plate, 18-constant current source, 19-detection module mount, 20-light source, 21-optical detection module, 22-backlight source, 23-gel card, 24-camera, 25-camera mount, 26-light source slide rail, 27-slide holder, 28-synchronous holder, 29-base, 30-synchronous belt, 31-first driving pulley, 32-synchronous motor, 33-synchronous slide rail, 34-slide table, 35-circuit board, 36-pair light pipe, 37-suction head box, 38-suction head, 39-sample pipe, 40-slide rail, 41-rail, 42-screw rod, 43-clamping motor, 44-slide block, 45-temperature sensor, 46-heating belt, 47-second driven pulley, 48-slide plate, 49-driving motor, 50-driving belt, 51-piercing bracket, 52-piercing motor, 53-eccentric wheel, 54-piercing slide rail, 55-piercing slide block, 56-tension spring, 57-gel cassette, 58-gel sleeve, 59-rotating rod, 60-a first driven pulley, 61-a second driving pulley, 62-a tool apron, 63-a telescopic motor, 64-a fourth driving pulley, 65-a fourth driven pulley, 66-a lifting block, 67-a third driving pulley, 68-a third driven pulley and 69-a rotating motor.

Detailed Description

The following detailed description of embodiments of the invention refers to the accompanying drawings.

As shown in FIGS. 1-7, a full-automatic platelet antibody detection analyzer comprises a control system, a circuit board 35, a base 29, a suction head storage module 1, a sample storage module, a gel card storage puncture module, a test waste discarding module 4, a centrifugal incubation module, a reagent storage module, a clamping manipulator module, a detection module, a beam module 10 and a sample feeding arm slide rail module, wherein the control system controls the circuit board 35 to operate, the circuit board 35 controls the operation of each module, the suction head storage module 1, the sample storage module, the gel card storage puncture module, the test waste discarding module 4, the centrifugal incubation module, the reagent storage module, the clamping manipulator module, the detection module and the beam module 10 are all fixedly connected to the base 29, and the suction head storage module 1, the sample storage module, the gel card storage puncture module, the reagent storage puncture module, the clamping manipulator module, the detection module and the beam module 10 are sequentially arranged from left to right along the length direction of the base 29, The test waste discarding module 4 and the centrifugal incubation module, the reagent storage module is positioned at the rear side of the suction head storage module 1, the circuit board 35 is positioned at the rear side of the reagent storage module 1, the clamping manipulator module is positioned at the rear side of the test waste discarding module 4, the detection module is positioned at the rear side of the centrifugal incubation module, the beam module 10 is used for supporting and driving the sample adding slide rail module to move, and comprises a sliding support 27 and a synchronous support 28, the length directions of the sliding support 27 and the synchronous support 28 are parallel to the length direction of the base 29 and are symmetrically arranged at the front end and the rear end of the base 29 respectively, the sample adding arm slide rail module is used for supporting and driving the electronic liquid transferring gun 12 to move, the front end of the sample adding arm slide rail module is connected to the sliding support 27 in a sliding manner, and the rear end of the sample adding arm slide rail module is arranged on the synchronous support 28; the sucker storage module 1 is of a drawer type push-pull structure, two sucker boxes 37 are arranged in parallel in the front and at the back of the sucker storage module, and a certain number of suckers 38 are arranged in the sucker boxes 37; six rows of sample racks 2 are arranged on the left and right of the sample storage module in parallel, each row of sample racks 2 can contain twelve sample tubes 39, and each row of sample racks 2 is provided with an independent slide way 40 in a matching way; the gel card storage puncturing module comprises a gel card holder 57, a track 41, a screw rod 42, a blocking motor 43, a slider 44 and a puncturing mechanism, wherein a certain number of gel card blocking positions 3 are arranged side by side in front and back of the top of the gel card holder 57 and used for storing gel cards 23, the bottom of the gel card holder is fixedly connected with the slider 44, the bottom surface of the slider 44 is abutted against the top surface of the track 41 and can slide relatively, the blocking motor 43 is fixedly connected with one end of the track 41, the output end of the blocking motor is fixedly connected with one end of the screw rod 42, the screw rod 42 penetrates through the slider 44 and is in threaded connection with the screw rod, the other end of the screw rod 42 is rotatably connected with the other end of the track 41, the puncturing mechanism is used for puncturing the gel cards 23 and is positioned above the middle part of the track 41 and comprises a puncturing bracket 51, a puncturing motor 52, an eccentric wheel 53, a puncturing slide rail 54, a puncturing slider 55, a tension spring 56 and a tool apron 62, the bottom end of the puncturing bracket 51 is fixedly connected on the base 29, the puncture motor 52 is fixedly connected to the upper part of the puncture bracket 51, the output end of the puncture motor is fixedly connected with an eccentric wheel 53, two puncture slide rails 54 are respectively and symmetrically fixedly connected to the middle part of the puncture bracket 51, each puncture slide rail 54 is connected with a puncture slide block 55 in a sliding way, the tool apron 62 is fixedly connected with the two puncture slide blocks 55, a certain number of puncture tools 7 are arranged on the tool apron 62, two tension springs 56 are respectively positioned in front of the two puncture slide rails 54, one end of each tension spring 56 is fixedly connected to the puncture bracket 51, and the other end of each tension spring 56 is fixedly connected to the tool apron 62; the test waste discarding module 4 is of a drawer-type push-pull structure and is used for containing the gel card 23 discarded after detection, and three pairs of light pipes 36 are arranged on two sides of the test waste discarding module 4 so as to monitor whether the test waste discarding module 4 is filled up or not; the centrifugal incubation module is used for incubating and centrifuging samples in the gel card 23 at constant temperature, and comprises a centrifuge 5 and a temperature control module, wherein the temperature control module comprises a heating belt 45 and a temperature sensor 45, so that the temperature condition in the centrifuge 5 can be monitored in real time, the gel card 23 is convenient to have a better action effect, the heating belt 46 is arranged on the bottom surface of the centrifuge 5, and the temperature sensor 45 is arranged at the top of the side surface of the centrifuge 5; the reagent storage module is provided with three reagent positions 6 in parallel at the left and right; the clamping manipulator module is used for clamping the moving clamp of the gel card 23, the clamping module mounting seat 14, a manipulator telescopic component, a manipulator mounting seat 16, a finger component 15, a manipulator rotating component and a rubber sleeve 58 are sequentially arranged from bottom to top, the manipulator telescopic component comprises a telescopic belt 8, a telescopic motor 63, a fourth pulley block and a lifting block 66, one side of the telescopic motor 63 is horizontally and fixedly connected to the bottom of the outer side of the clamping module mounting seat 14, the output end of the telescopic motor 63 penetrates through the clamping module mounting seat 14, the fourth pulley block comprises a fourth driving pulley 64 and a fourth driven pulley 65, the fourth driving pulley 64 is fixedly connected to the output end of the telescopic motor 63, the fourth driven pulley 65 is mounted at the top end of the inner side of the clamping module mounting seat 14, the inner side of the telescopic belt 8 is connected with the fourth pulley block in a matched mode, the lifting block 66 is fixedly connected with one side of the telescopic belt 8, the manipulator rotating component comprises a rotating rod 59, The rotating belt 13, the rotating motor 69 and a third pulley block, one side of the rotating motor 69 is vertically and fixedly connected to the upper part of the clamping module mounting seat 14, the output end of the rotating motor runs through the clamping module mounting seat 14, two ends of the rotating rod 59 are respectively and rotatably connected with the clamping module mounting seat 14, the third driven pulley block comprises a third driving pulley 67 and a third driven pulley 68, the third driving pulley 67 is fixedly connected to the output end of the rotating motor 69, the third driven pulley 68 is fixedly connected to the top end of the rotating rod 59, a rubber sleeve 58 is fixedly sleeved in one end of the manipulator mounting seat 16, a finger component 15 is fixedly connected to the top surface of the other end of the manipulator mounting seat and used for clamping the gel card 23, and the rotating rod 59 runs through the rubber sleeve 58 and is in sliding friction connection with the rubber sleeve 58; the detection module is used for photographing and interpreting samples in the gel card 23 and detecting fluorescence intensity, and comprises a fixing plate 17, a constant current source 18, a detection module mounting frame 19, a light source 20, an optical detection module 21, an optical darkroom 9, a camera 24, a camera fixing seat 25, a light source slide rail 26 and a backlight source 22, the constant current source 18, the detection module mounting frame 19, the camera fixing seat 25, the light source slide rail 26 and the backlight source 22 are fixedly connected to the fixing plate 17, the light source 20 is electrically connected with the constant current source 18, the optical darkroom 9 is fixedly connected to the light source slide rail 26, the optical detection module 21 is fixedly connected to the detection module mounting frame 19, and the camera 24 is fixedly connected to one side face of the camera fixing seat 25; the synchronous support 28 is also provided with a synchronous belt 30, a first pulley block, a synchronous motor 32, a synchronous sliding rail 33 and a sliding table 34, the bottom end of the synchronous motor 32 is fixedly connected to the upper portion of the right end of the synchronous support 28, the first pulley block comprises a first driving pulley 31 and a first driven pulley 60, the first driving pulley 31 is fixedly connected with the output end of the synchronous motor 32, the first driven pulley 60 is mounted at the top end of the left side of the synchronous support 28, the inner side of the synchronous belt 30 is connected with the first pulley block in a matched manner, the synchronous sliding rail 33 is arranged on the rear side of the synchronous belt 30, the sliding table 34 is connected with the synchronous sliding rail 33 in a sliding manner, and one side of the sliding table 34 is fixedly connected with the synchronous belt 30; the sample adding arm slide rail module comprises a sample adding arm slide rail 11 and three electronic liquid-transfering guns 12 which are arranged side by side front and back, the bottom of the front end of the sample adding arm slide rail 11 is connected on a sliding support 27 in a sliding way, the bottom of the back end of the sample adding arm slide rail is fixedly connected with the top surface of a sliding table 34, the upper part of the inner side of the sample adding arm slide rail 11 is also provided with three groups of transmission mechanisms which are arranged side by side up and down, taking one group as an example, each transmission mechanism comprises a second pulley block, a sliding plate 48, a transmission motor 49 and a transmission belt 50, the second pulley block comprises a second driving pulley 61 and a second driven pulley 47, the second driven pulley 47 is arranged at the front end of the inner side of the sample adding arm slide rail 11, one side of the transmission motor 49 is fixedly connected with the rear end of the inner side of the sample adding arm slide rail 11, the output end of the second driving pulley 61 is fixedly connected with the inner side of the transmission belt 50, the inner side of the second pulley block is connected with the sample adding arm slide rail 11 in a sliding way, which penetrates through the transmission belt 50 and is fixedly connected with one side of the transmission belt 50, and the other side of the sliding plate 48 is fixedly connected with the electronic pipette 12 for filling samples and reagents.

The full-automatic platelet antibody detection analyzer and the use method thereof comprise the following steps:

(1) before detection, an operator puts a suction head 38 into a suction head box 37 (the system automatically records the balance of the suction head 38 detected last time), puts platelet suspension, diluent and fluorescent reagent into three reagent positions 6 of a reagent storage module from left to right in sequence, puts a sample tube 39 into a sample rack 2, and puts a corresponding number of gel cards 23 (six persons per gel card 23) into a gel card storage area according to the number of the sample tubes 2 to be detected;

(2) setting parameters such as the number of detected samples, incubation temperature, incubation time, centrifugation speed, centrifugation time and the like (the parameters can be named and stored) by using a control system;

(3) the control system carries out the following operation instructions according to the number of samples:

firstly, transferring the gel card 23 to a gel card storage puncturing module to puncture a sealing film;

secondly, adding platelet suspension, diluent, fluorescent reagent and a patient plasma sample into the gel card 23 in sequence by using a sample adding arm sliding rail module;

grabbing the gel card 23 from the gel card storage area by using a clamping manipulator module, and putting the gel card into a centrifugal incubation module; repeating the clamping action, and putting all the gel cards 23 added with the samples into a centrifugal incubation module;

fourthly, after the gel card 23 is incubated at constant temperature and centrifuged, the clamping manipulator module is put into the detection module;

fifthly, after entering the detection module, the gel card 23 is photographed and interpreted, then is transmitted to the optical darkroom 9 through the light source slide rail 26 to be subjected to fluorescence intensity detection, and gives qualitative and quantitative detection results through an image processing algorithm and an A/D amplification algorithm;

sixthly, after the gel card detection 23 is finished, the clamping manipulator module is used for grabbing the gel card 23, the gel card 23 is rotated to the test waste discarding module 4 to be discarded, the clamping manipulator module is used for grabbing the gel card 23 from the centrifugal incubation module and then putting the gel card into the detection module for detection, and the steps are repeated until all detection is finished.

Examples

As shown in fig. 1 to 7, before detection, an operator puts a suction head 38 into a suction head box 37 (the system automatically records the residual amount of the suction head 38 detected last time), puts platelet suspension, diluent and fluorescent reagent into three reagent positions 6 of a reagent storage module from left to right in sequence, puts a sample tube 39 into a sample rack 2, and puts a corresponding number of gel cards 23 (six gel cards 23 per gel card) into a gel card storage area according to the number of the sample tubes 2 to be detected; setting parameters such as the number of detected samples, incubation temperature, incubation time, centrifugation speed, centrifugation time and the like (the parameters can be named and stored) by using a control system; the control system carries out the following operation instructions according to the number of samples: transferring the gel card 23 to the gel card storage puncturing module to puncture the sealing film; sequentially adding platelet suspension, diluent, fluorescent reagent and a patient plasma sample into the gel card 23 by using the sample adding arm slide rail module; grabbing the gel card 23 from the gel card storage area by using a clamping manipulator module, and putting the gel card into a centrifugal incubation module; repeating the clamping action, and putting all the gel cards 23 added with the samples into a centrifugal incubation module; after the gel card 23 is incubated at a constant temperature and centrifuged, placing the gel card into a detection module by using a card clamping manipulator module; after entering the detection module, the gel card 23 is photographed and interpreted, then transmitted into the optical darkroom 9 through the light source slide rail 26 for fluorescence intensity detection, and gives qualitative and quantitative detection results through an image processing algorithm and an A/D amplification algorithm; after a gel card is detected 23, the clamping manipulator module is used for grabbing the gel card 23, the gel card is rotated to the test waste discarding module 4 to be discarded, the clamping manipulator module is used for grabbing the gel card 23 from the centrifugal incubation module and putting the gel card into the detection module to be detected, and the steps are repeated until all the detection is completed.

Claims (3)

1. The full-automatic platelet antibody detection analyzer is characterized by comprising a control system, a circuit board, a base, a suction head storage module, a sample storage module, a gel card storage puncture module, a test waste discarding module, a centrifugal incubation module, a reagent storage module, a clamping manipulator module, a detection module, a beam module and a sample adding arm slide rail module, wherein the control system controls the circuit board to operate, the circuit board controls the action of each module, the suction head storage module, the sample storage module, the gel card storage puncture module, the test waste discarding module, the centrifugal incubation module, the reagent storage module, the clamping manipulator module, the detection module and the beam module are all fixedly connected to the base, and the suction head storage module, the sample adding manipulator module and the beam module are sequentially arranged from left to right along the length direction of the base, The gel card storage puncture module, the test waste discarding module and the centrifugal incubation module are arranged, the reagent storage module is located on the rear side of the suction head storage module, the circuit board is located on the rear side of the reagent storage module, the clamping manipulator module is located on the rear side of the test waste discarding module, the detection module is located on the rear side of the centrifugal incubation module, the beam module comprises a sliding support and a synchronous support, the length directions of the sliding support and the synchronous support are parallel to the length direction of the base and are symmetrically arranged at the front end and the rear end of the base respectively, the front end of the sample adding arm sliding rail module is connected to the sliding support in a sliding mode, and the rear end of the sample adding arm sliding rail module is installed on the synchronous support.

2. The fully automatic platelet antibody detection analyzer and the using method thereof according to claim 1, wherein the suction head storage module is a drawer-type push-pull structure, two suction head boxes are arranged in front and at the back side by side, and a certain number of suction heads are arranged in the suction head boxes;

the sample storage module is provided with six rows of sample racks side by side on the left and right, each row of sample racks can contain twelve sample tubes, and each row of sample racks is provided with an independent slide way in a matching way;

the gel card storage puncturing module comprises a gel card clamping seat, a track, a screw rod, a clamping motor, a sliding block and a puncturing mechanism, wherein a certain number of gel card clamping positions are arranged in parallel in the front and at the back of the top of the gel card clamping seat, the bottom of the gel card clamping seat is fixedly connected with the sliding block, the bottom surface of the sliding block is abutted against the top surface of the track and can slide relatively, the clamping motor is fixedly connected to one end of the track, the output end of the clamping motor is fixedly connected with one end of the screw rod, the screw rod penetrates through the sliding block and is in threaded connection with the sliding block, the other end of the screw rod is rotatably connected with the other end of the track, the puncturing mechanism is positioned above the middle of the track and comprises a puncturing bracket, a puncturing motor, an eccentric wheel, a puncturing slide rail, a puncturing sliding block, a puncturing spring and a knife holder, the bottom end of the puncturing bracket is fixedly connected to the base, the puncturing motor is fixedly connected to the upper part of the puncturing bracket, and the output end of the eccentric wheel is fixedly connected to the puncturing motor, the number of the puncture slide rails is two, the puncture slide rails are respectively and symmetrically fixedly connected to the middle of the puncture bracket, each puncture slide rail is connected with one puncture slide block in a sliding manner, the tool apron is fixedly connected with the two puncture slide blocks, a certain number of puncture knives are arranged on the tool apron, two tension springs are arranged in front of the two puncture slide rails respectively, one end of each tension spring is fixedly connected to the puncture bracket, and the other end of each tension spring is fixedly connected to the tool apron;

the test waste discarding module is of a drawer type push-pull structure, and three pairs of light pipes are arranged on two sides of the test waste discarding module so as to monitor whether the test waste discarding module is filled;

the centrifugal incubation module comprises a centrifuge and a temperature control module, the temperature control module comprises a heating belt and a temperature sensor, the heating belt is arranged on the bottom surface of the centrifuge, and the temperature sensor is arranged at the top of the side surface of the centrifuge;

the reagent storage module is provided with three reagent positions on the left and right side in parallel;

the clamping manipulator module is sequentially provided with a clamping module mounting seat, a manipulator telescopic component, a manipulator mounting seat, a finger component, a manipulator rotating component and a rubber sleeve from bottom to top, the manipulator telescopic component comprises a telescopic belt, a telescopic motor, a fourth pulley block and a lifting block, one side of the telescopic motor is horizontally and fixedly connected to the bottom outside the clamping module mounting seat, the output end of the telescopic motor penetrates through the clamping module mounting seat, the fourth pulley block comprises a fourth driving pulley and a fourth driven pulley, the fourth driving pulley is fixedly connected to the output end of the telescopic motor, the fourth driven pulley is mounted at the top end of the inner side of the clamping module mounting seat, the inner side of the telescopic belt is connected with the fourth pulley block in a matching manner, the lifting block is fixedly connected with one side of the telescopic belt, and the manipulator rotating component comprises a rotary rod, a rotary belt, a rubber sleeve, The manipulator comprises a rotating motor and a third pulley block, wherein one side of the rotating motor is vertically and fixedly connected to the upper part of a clamping module mounting seat, the output end of the rotating motor penetrates through the clamping module mounting seat, two ends of a rotating rod are respectively and rotatably connected with the clamping module mounting seat, the third driven pulley block comprises a third driving pulley and a third driven pulley, the third driving pulley is fixedly connected to the output end of the rotating motor, a third driven pulley is fixedly connected to the top end of the rotating rod, a rubber sleeve is fixedly sleeved in one end of the manipulator mounting seat, a finger component is fixedly connected to the top surface of the other end of the manipulator mounting seat, and the rotating rod penetrates through the rubber sleeve and is in sliding friction connection with the rubber sleeve;

the detection module comprises a fixed plate, a constant current source, a detection module mounting frame, a light source, an optical detection module, an optical darkroom, a camera fixing seat, a light source sliding rail and a backlight source, wherein the constant current source, the detection module mounting frame, the camera fixing seat, the light source sliding rail and the backlight source are all fixedly connected to the fixed plate;

the synchronous support is further provided with a synchronous belt, a first pulley block, a synchronous motor, a synchronous slide rail and a sliding table, the bottom end of the synchronous motor is fixedly connected to the upper portion of the right end of the synchronous support, the first pulley block comprises a first driving pulley and a first driven pulley, the first driving pulley is fixedly connected with the output end of the synchronous motor, the first driven pulley is mounted at the top end of the left side of the synchronous support, the inner side of the synchronous belt is connected with the first pulley block in a matched mode, the synchronous slide rail is arranged on the rear side of the synchronous belt, the sliding table is connected with the synchronous slide rail in a sliding mode, and one side of the sliding table is fixedly connected with the synchronous belt;

the sample adding arm slide rail module comprises a sample adding arm slide rail and three electronic liquid-transfering guns arranged side by side from front to back, the bottom of the front end of the sample adding arm slide rail is connected to the sliding support in a sliding way, the bottom of the back end of the sample adding arm slide rail is fixedly connected with the top surface of the sliding table, and the upper part of the inner side of the sample adding arm slide rail is also provided with three groups of transmission mechanisms arranged side by side from top to bottom, taking one group as an example, each transmission mechanism comprises a second pulley block, a slide plate, a transmission motor and a transmission belt, the second pulley block comprises a second driving pulley and a second driven pulley, the second driven pulley is arranged at the front end of the inner side of the sample adding arm slide rail, one side of the transmission motor is fixedly connected with the rear end of the inner side of the sample adding arm slide rail, the inner side of the transmission belt is connected with the second pulley block in a matching way, one side of the slide plate is connected with the sample adding arm slide rail in a sliding way, and penetrates through the transmission belt and is fixedly connected with one side of the transmission belt, the other side of the sliding plate is fixedly connected with the electronic liquid-transfering gun.

3. The fully automatic platelet antibody detection analyzer and its use method according to claim 1, wherein the use method comprises the following steps:

(1) before detection, an operator puts a suction head into a suction head box (the system automatically records the balance of the suction head detected last time), puts platelet suspension, diluent and fluorescent reagent into three reagent positions of a reagent storage module from left to right in sequence, puts sample tubes into a sample rack, and puts a corresponding number of gel cards (six gel cards per gel card) into a gel card storage area according to the number of the sample tubes to be detected;

(2) setting parameters such as the number of detected samples, incubation temperature, incubation time, centrifugation speed, centrifugation time and the like (the parameters can be named and stored) by using a control system;

(3) the control system carries out the following operation instructions according to the number of samples:

firstly, transferring the gel card to a gel card storage puncturing module to puncture a sealing film;

sequentially adding platelet suspension, diluent, fluorescent reagent and a patient plasma sample into the gel card by using the sample adding arm sliding rail module;

grabbing the gel card from the gel card storage area by using a clamping manipulator module, and putting the gel card into a centrifugal incubation module; repeating the clamping action, and putting all the gel cards added with the samples into a centrifugal incubation module;

fourthly, placing the gel card into the detection module by using the clamping manipulator module after incubation and centrifugation at constant temperature;

fifthly, after entering the detection module, the gel card is photographed and interpreted, then is transmitted to an optical darkroom through a light source slide rail to be detected in fluorescence intensity, and qualitative and quantitative detection results are given through an image processing algorithm and an A/D amplification algorithm;

sixthly, after the detection of one gel card is finished, the clamping manipulator module is used for grabbing the gel card, the gel card is rotated to the test waste discarding module to be discarded, the clamping manipulator module is used for grabbing the gel card from the centrifugal incubation module and placing the gel card into the detection module for detection, and the steps are repeated until all detection is finished.

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