CN216082592U - Pretreatment device for microbial mass spectrometry detection - Google Patents
Pretreatment device for microbial mass spectrometry detection Download PDFInfo
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- CN216082592U CN216082592U CN202121521621.6U CN202121521621U CN216082592U CN 216082592 U CN216082592 U CN 216082592U CN 202121521621 U CN202121521621 U CN 202121521621U CN 216082592 U CN216082592 U CN 216082592U
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Abstract
The utility model discloses a pretreatment device for microbial mass spectrometry detection, which comprises: the sample preparation device comprises a rack, wherein a sample preparation station for preparing a sample is arranged on the rack; the feeding mechanism is arranged on the rack and can convey the culture dish to a sample preparation station and separate the cover body and the dish body of the culture dish conveyed to the sample preparation station; the sampling mechanism is arranged at the sample preparation station and can sample microorganisms in a culture dish conveyed to the sample preparation station; and the sample preparation mechanism is arranged on the sample preparation station and can be matched with the sampling mechanism to prepare samples. The pretreatment device for microbial mass spectrometry detection has high automation degree and high sample flux during working, and can meet the clinical requirement of performing microbial mass spectrometry identification quickly and efficiently.
Description
Technical Field
The utility model relates to the technical field of microbial detection, in particular to a pretreatment device for microbial mass spectrum detection.
Background
The main task of a clinical microorganism laboratory is to assist a clinician to diagnose and identify various infectious diseases and guide the clinical timely and reasonable administration of the medicines, and the culture of pathogenic microorganisms usually needs a long time and is easy to delay the timely treatment of a patient, so that the rapid and accurate identification of infected strains is the key for determining whether the patient can be treated timely. The pretreatment of the microorganism specimen is an important link in the inspection, the workload is large, the time spent is long, the operator faces the risk of biological infection, and the manual operation is greatly influenced by human factors, so that the accuracy of all subsequent detections is directly influenced. Therefore, a full-automatic microbial sample processing system with standard processing capacity is needed in the market, which can complete the full-automatic, fast and safe inoculation of bacterial colonies from a culture dish to a MALDI-TOF MS target plate, and the lysis and immobilization processing.
In the prior art, the mass spectrometric detection procedure of microorganisms generally comprises: culturing microorganisms by adopting a culture dish, preparing a sample by utilizing the microorganisms cultured in the culture dish, carrying out spectrogram acquisition on the prepared sample, comparing the acquired data with a database and issuing an identification result. Some existing pretreatment devices for microbial mass spectrometry detection are on the market, but the pretreatment devices for microbial mass spectrometry detection still need manual operation in the sample preparation process, the automation degree is low, the preparation time of a target plate is long, so that the sample flux of the pretreatment devices for microbial mass spectrometry detection is low, and the clinical requirement for performing microbial mass spectrometry identification quickly and efficiently cannot be met.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the utility model provides a pretreatment device for microbial mass spectrometry with high automation degree, which has high sample flux during working and can meet the clinical requirement of performing microbial mass spectrometry identification quickly and efficiently.
The pretreatment device for microbial mass spectrometry detection provided by the embodiment of the utility model comprises: the sample preparation device comprises a rack, wherein a sample preparation station for preparing a sample is arranged on the rack; the feeding mechanism is arranged on the rack and can convey the culture dish to a sample preparation station and separate the cover body and the dish body of the culture dish conveyed to the sample preparation station; the sampling mechanism is arranged at the sample preparation station and can sample microorganisms in a culture dish conveyed to the sample preparation station; and the sample preparation mechanism is arranged on the sample preparation station and can be matched with the sampling mechanism to prepare samples.
The pretreatment device for microbial mass spectrometry provided by the embodiment of the utility model has at least the following beneficial effects: during the use, through feed mechanism will cultivate the dish carry extremely system appearance station will carry extremely the lid and the dish body separation of the culture dish of system appearance station, then pass through sampling mechanism is to carrying extremely microorganism in the culture dish of system appearance station is sampled, at last through system appearance mechanism cooperation sampling mechanism prepares the sample that is used for mass spectrometry. The pretreatment device for microbial mass spectrometry detection has high automation degree and high sample flux during working, and can meet the clinical requirement of performing microbial mass spectrometry identification quickly and efficiently.
According to some embodiments of the utility model, the feed mechanism comprises: the storage component is arranged on the rack and used for placing a culture dish; the feeding assembly is arranged on the rack and can convey the culture dish placed on the storage assembly to the sample preparation station; and the separation assembly is arranged on the rack and can be matched with the feeding assembly to convey the culture dish to the sample preparation station, so that the cover body and the dish body are separated.
According to some embodiments of the utility model, the storage component comprises a base for placing the culture dish, the base is arranged on the rack and located below the sample preparation station, a limiting rod for limiting the peripheral side of the culture dish is arranged on the base, the feeding component comprises a first driving device and an ejector, the ejector is arranged on the rack in a vertically sliding manner and located below the base, the first driving device is arranged on the rack and can drive the ejector to lift relative to the rack, a first avoidance opening for avoiding the ejector is arranged on the base, and the limiting rod is arranged on the peripheral side of the first avoidance opening.
According to some embodiments of the utility model, the separation assembly is disposed at the sample preparation station, the separation assembly includes a sliding seat and clamping assemblies, the sliding seat is slidably disposed on the rack, a second avoidance port for avoiding a culture dish is disposed on the sliding seat, two sets of the clamping assemblies are disposed on the sliding seat, a second driving device is disposed on the rack corresponding to the sliding seat, and the second driving device can drive the sliding seat to slide relative to the rack and move one of the two sets of the clamping assemblies to a position right above the pushing member.
According to some embodiments of the utility model, the clamping assembly comprises two clamping portions movably arranged on the sliding seat and a third driving device arranged on the sliding seat corresponding to the two clamping portions, and the third driving device can drive the two clamping portions to approach or move away from each other.
According to some embodiments of the utility model, a buffer is arranged between two clamping parts of the same group of clamping assemblies.
According to some embodiments of the utility model, the sampling mechanism comprises: the three-axis manipulator comprises an X-axis linear module arranged on the rack, a Y-axis linear module arranged on the X-axis linear module and a first Z-axis linear module arranged on the Y-axis linear module, wherein a grabbing mechanism is arranged on the first Z-axis linear module and can grab a swab used for sampling to sample; the visual positioning assembly, the visual positioning assembly set up in the frame and be located the top of system appearance station, the distribution information of the bacterial colony of microorganism in the culture dish can be acquireed to the visual positioning assembly.
According to some embodiments of the utility model, the three-axis robot is provided with a laser displacement sensor for detecting a colony height of the microorganism in the culture dish.
According to some embodiments of the utility model, the sample preparation mechanism comprises: the first placing frame is arranged on the rack and used for placing a target plate; the second placing frame is arranged on the rack and is used for placing reagent bottles; the second Z-axis linear module is arranged on the Y-axis linear module, and a needle capable of injecting liquid or extracting liquid is arranged on the second Z-axis linear module.
According to some embodiments of the utility model, a first scanner for recording the number of the target plate is provided on the rack corresponding to the sample preparation mechanism.
Additional aspects and advantages of the utility model will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the utility model.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1 at A;
FIG. 3 is a schematic structural view of a magazine assembly according to an embodiment of the present invention;
FIG. 4 is a schematic structural view of a feed assembly according to an embodiment of the present invention;
FIG. 5 is a schematic structural view of a separator assembly according to an embodiment of the utility model;
FIG. 6 is a schematic structural diagram of a three-axis robot in accordance with an embodiment of the present invention;
FIG. 7 is a schematic diagram of a visual alignment assembly according to an embodiment of the present invention;
FIG. 8 is a first schematic structural diagram of a workbench according to an embodiment of the present invention;
fig. 9 is a schematic structural diagram of a second working platform according to an embodiment of the present invention.
Reference numerals:
the robot comprises a rack 100, a workbench 110, a first placing frame 111, a second placing frame 112, a guide channel 113, a material storage assembly 200, a base 210, a limiting rod 211, a fourth driving device 220, a reflecting mirror 230, a photoelectric sensor 240, a feeding assembly 300, a first driving device 310, a pushing piece 320, a pushing plate 321, a separating assembly 400, a sliding seat 410, a second avoidance port 411, a second driving device 420, a clamping part 430, an arc-shaped concave part 431, a third driving device 440, a buffering piece 450, a three-axis manipulator 500, an X-axis linear module 510, a Y-axis linear module 520, a first Z-axis linear module 530, a laser displacement sensor 540, a second Z-axis linear module 550, a needle 551, a second camera 560, a visual positioning assembly 600, a first camera 610, a light source 620, a first scanner 700, an injection pump 810, a wide-mouth bottle 820 and a second scanner 900.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that if an orientation description is referred to, for example, the directions or positional relationships indicated by upper, lower, front, rear, left, right, etc., are based on the directions or positional relationships shown in the drawings, it is only for convenience of description and simplification of description, but it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, if several, more than, less than, more than, above, below, or within words appear, several means are one or more, several means are two or more, more than, less than, more than, etc. are understood as not including the number, and more than, less than, within, etc. are understood as including the number.
In the description of the present invention, if the first, second, etc. terms appear, they are only used for distinguishing technical features, but are not to be interpreted as indicating or implying relative importance or implying number of indicated technical features or implying precedence of indicated technical features.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
Referring to fig. 1, the pretreatment device for microbial mass spectrometry according to the embodiment of the present invention includes a rack 100, a feeding mechanism, a sampling mechanism, and a sample preparation mechanism.
Be provided with the system appearance station that is used for preparing the sample on the frame 100, feed mechanism sets up on frame 100, feed mechanism can carry the culture dish to system appearance station and can be with carrying lid and the separation of the ware body to the culture dish of system appearance station, sampling mechanism sets up in system appearance station, sampling mechanism can take a sample to the microorganism of carrying in the culture dish to system appearance station, system appearance mechanism sets up in system appearance station, system appearance mechanism can cooperate sampling mechanism to prepare the sample.
During the use, carry the culture dish to system appearance station and will carry lid and the separation of dish body to the culture dish of system appearance station through feed mechanism, then sample the microorganism in carrying the culture dish to system appearance station through sampling mechanism, prepare the sample that is used for mass spectrometry through system appearance mechanism cooperation sampling mechanism at last. The pretreatment device for microbial mass spectrometry detection has high automation degree and high sample flux during working, and can meet the clinical requirement of performing microbial mass spectrometry identification quickly and efficiently.
Referring to FIG. 1, in some embodiments thereof, the feed mechanism includes a magazine assembly 200, a feed assembly 300, and a separator assembly 400.
Referring to fig. 1, 3 and 4, in some embodiments, the storage assembly 200 includes a base 210 for placing a culture dish, the base 210 is disposed on the rack 100 and located below the sample preparation station, a limiting rod 211 for limiting the circumferential side of the culture dish is disposed on the base 210, the feeding assembly 300 includes a first driving device 310 and an ejector 320, the ejector 320 is slidably disposed on the rack 100 and located below the base 210, the first driving device 310 is disposed on the rack 100 and can drive the ejector 320 to move up and down relative to the rack 100, a first avoidance opening for avoiding the ejector 320 is disposed on the base 210, and the limiting rod 211 is disposed on the circumferential side of the first avoidance opening. A plurality of culture dishes of waiting to carry correspond first dodge the mouth and stack on base 210 along vertical direction one by one, when needing to carry the culture dish to system appearance station, rise through first drive arrangement 310 drive pusher 320, pusher 320 passes first dodge the mouth and bears in the bottom that is located the culture dish of below, along with pusher 320 continues to rise, the culture dish that is located the top is carried to system appearance station, wherein, gag lever post 211 can prevent to correspond a plurality of culture dishes of waiting to carry that first dodge the mouth and stack one by one along vertical direction and take place to empty at the in-process that rises, its simple structure and easily realization.
It should be noted that, in some embodiments, the first driving device 310 is a motor, and the motor is in transmission connection with the pushing member 320 and can drive the pushing member 320 to ascend and descend relative to the machine frame 100, where the motor may be in transmission connection with the pushing member 320 through a rack-and-pinion structure, or may be in transmission connection with the pushing member 320 through a screw mechanism, and of course, the first driving device may also be an electric push rod or an air cylinder connected to the pushing member, which is not limited herein.
Referring to fig. 4, in some embodiments, a push plate 321 is disposed on the top of the pushing member 320, so that a contact area between the pushing member 320 and the culture dish is increased, the culture dish can be prevented from shaking during the transportation process, and the stability of the transportation process can be improved.
Referring to fig. 1 and 5, in some embodiments, the separation assembly 400 is disposed at a sample preparation station, the separation assembly 400 includes a sliding seat 410 and clamping assemblies, the sliding seat 410 is slidably disposed on the rack 100, a second avoidance port 411 for avoiding a culture dish is disposed on the sliding seat 410, two sets of clamping assemblies are disposed on the sliding seat 410, a second driving device 420 is disposed on the rack 100 corresponding to the sliding seat 410, and the second driving device 420 can drive the sliding seat 410 to slide relative to the rack 100 and move one of the two sets of clamping assemblies to a position directly above the pushing member 320. During operation, the sliding seat 410 is driven by the second driving device 420 to slide relative to the frame 100, so that one of the two sets of clamping assemblies moves to a position right above the pushing member 320, then the pushing member 320 is driven by the first driving device 310 to ascend, the pushing member 320 passes through the first avoiding opening and bears on the bottom of the culture dish positioned at the lowest position, as the pushing member 320 continues to ascend, the culture dish positioned at the uppermost position is conveyed to the sample preparation station and passes through the second avoiding opening 411, at this time, the clamping assembly positioned right above the pushing member 320 clamps the cover body of the culture dish positioned at the uppermost position, then the pushing member 320 is driven by the first driving device 310 to descend, so that the dish body of the culture dish positioned at the uppermost position is separated from the cover body, at this time, the sliding seat 410 is driven by the second driving device 420 to slide relative to the frame 100, so that the other set of clamping assemblies moves to a position right above the pushing member 320, then, the pushing member 320 is driven by the first driving device 310 to ascend again, so that the dish body of the uppermost culture dish is conveyed to the sample preparation station again and passes through the second avoiding opening 411, and at this time, the dish body of the uppermost culture dish is clamped by the clamping assembly located right above the pushing member 320, so that the dish body of the culture dish is separated from the cover body.
It should be noted that, in some embodiments, the second driving device 420 is a motor, and the motor is in transmission connection with the sliding seat 410, wherein the motor may be in transmission connection with the sliding seat 410 through a rack-and-pinion structure, and may also be in transmission connection with the sliding seat 410 through a screw rod mechanism, and of course, the second driving device may also be an electric push rod or an air cylinder connected to the sliding seat, which is not limited herein.
Referring to fig. 5, in some embodiments, the clamping assembly includes two clamping portions 430 movably disposed on the sliding seat 410 and a third driving device 440 disposed on the sliding seat 410 corresponding to the two clamping portions 430, and the third driving device 440 can drive the two clamping portions 430 to approach or move away from each other, which is simple in structure and easy to implement.
It should be noted that, in some embodiments, the third driving device 440 is a motor, in the same group of clamping assemblies, the motor is in transmission connection with the two clamping portions 430 through a lead screw mechanism, a screw of the lead screw mechanism has two screw thread sections with opposite thread directions, and the two clamping portions 430 are respectively connected to the two screw thread sections with opposite thread directions through nut seats, so that the two clamping portions 430 can approach or separate from each other when the screw rotates, of course, the third driving device may also be a clamping jaw air cylinder, and the two clamping portions are connected to the two clamping jaws of the clamping jaw air cylinder in a one-to-one correspondence, which is not limited herein.
Referring to fig. 5, in some embodiments, the clamping portion 430 is provided with an arc-shaped recess 431 adapted to the shape of the culture dish so as to clamp the cover or dish body of the culture dish.
Referring to fig. 5, in some embodiments, a buffer 450 is disposed between the two clamping portions 430 of the same clamping assembly to prevent the two clamping portions 430 of the same clamping assembly from excessively clamping the cover or dish body of the culture dish.
It should be noted that in some embodiments, the buffer member 450 is a compression spring, but of course, the buffer member may also be a rubber block or a sponge block, which is not limited herein.
Referring to fig. 3, in some embodiments, the base 210 is rotatably disposed on the rack 100, a fourth driving device 220 is disposed on the rack 100 corresponding to the base 210, the fourth driving device 220 can drive the base 210 to rotate relative to the rack 100, a plurality of first avoidance openings capable of rotating to a position right above the pushing member 320 are disposed on the base 210, and a limiting rod 211 is disposed on a peripheral side of each first avoidance opening. Correspond every first mouth of dodging and all stacked a plurality of culture dishes along vertical direction one by one, after the culture dish of one of them first mouth of dodging department has carried, drive base 210 through fourth drive arrangement 220 and rotate, so that another first mouth of dodging of stacking the culture dish rotates and continues to system appearance station transport culture dish to pushing part 320 directly over, meanwhile, can stack the culture dish of treating the transport to the first mouth of dodging that has not had the culture dish to stack through manipulator or manual work, be favorable to further improving machining efficiency.
It should be noted that, in some embodiments, the fourth driving device 220 is a motor, and the motor is in transmission connection with the base 210 and can drive the base 210 to rotate relative to the machine frame 100, wherein the motor may be in transmission connection with the base 210 through a gear structure, and may also be in transmission connection with the base 210 through a timing belt, which is not limited herein.
In some embodiments, a detection assembly is disposed on the rack 100 corresponding to the base 210, and the detection assembly can detect whether there is a culture dish to be transported directly above the pushing member 320. When the detection assembly detects that there is no culture dish to be conveyed right above the pushing member 320, the second driving device 220 drives the base 210 to rotate, so that the other first avoiding opening on which the culture dish is stacked rotates to the position right above the pushing member 320 to continuously convey the culture dish to the sample preparation station.
Referring to fig. 3, it should be noted that in some embodiments, the detecting assembly includes a reflecting mirror 230 and a photoelectric sensor 240 disposed on the rack 100, and the reflecting mirror 230 and the photoelectric sensor 240 can cooperate with each other to detect whether there is a culture dish to be transported directly above the pushing member 320.
Referring to fig. 1, 6 and 7, in some embodiments thereof, the sampling mechanism includes a three-axis robot 500 and a visual positioning assembly 600.
Referring to fig. 7, in some embodiments, the visual positioning assembly 600 specifically includes a first camera 610 and a light source 620, and the distribution information of the colonies of microorganisms in the culture dish is obtained through the pictures taken by the first camera 610, wherein the light source 620 facilitates the first camera 610 to take clearer pictures, and the structure is simple and easy to implement.
Referring to fig. 6, in some embodiments, the three-axis robot 500 is provided with a laser displacement sensor 540 for detecting the colony height of the microorganism in the culture dish, so that the sampling accuracy of the sampling mechanism can be further improved.
Referring to fig. 1, 2 and 6, in some embodiments thereof, the sample preparation mechanism includes a first rack 111, a second rack 112 and a second Z-axis linear module 550.
The first placing frame 111 is arranged on the rack 100 and used for placing the target plate, the second placing frame 112 is arranged on the rack 100 and used for placing the reagent bottle, the second Z-axis linear module 550 is arranged on the Y-axis linear module 520, and the second Z-axis linear module 550 is provided with a needle 551 capable of injecting liquid or extracting liquid. During operation, smear the microbial community on the swab to the target plate of placing on first rack 111 through first Z axle straight line module 530 cooperation X axle straight line module 510 and Y axle straight line module 520, then transfer lysate and matrix in the reagent bottle to the target plate of smearing the microbial community with preparation in order to be used for the sample of mass spectrometry through second Z axle straight line module 550 cooperation X axle straight line module 510 and Y axle straight line module 520 respectively.
Referring to fig. 2, in some embodiments, a first scanner 700 for recording the number of target plates is provided on the rack 100 corresponding to the sample preparation mechanism, so as to match the results of mass spectrometry detection with the prepared samples.
Referring to fig. 6, in some embodiments, the triaxial manipulator 500 is provided with a second camera 560 for monitoring operation results of the sampling mechanism and the sample preparation mechanism, and the operator can timely find out the misoperation and take corresponding measures through the feedback of the second camera 560 on the operation results.
Referring to fig. 1, in some embodiments, the rack 100 includes a working platform 110 disposed at the sample preparation station, the sampling mechanism, the sample preparation mechanism, and the separation assembly 400 are disposed on the working platform 110, a third avoidance port for avoiding the culture dish is disposed on the working platform 110 corresponding to the second avoidance port 411, and the sampling mechanism, the sample preparation mechanism, and the separation assembly 400 are integrated on the working platform 110, which is beneficial to reducing the overall volume of the whole apparatus.
Referring to fig. 8 and 9, in some embodiments, the working platform 110 is provided with a guide channel 113 at the third avoiding port for guiding the culture dish, which is beneficial to ensure that the culture dish conveyed to the sample preparation station and passing through the second avoiding port 411 is in a horizontal state, so that the clamping assembly clamps the culture dish cover or the dish body.
Referring to fig. 1 and 8, in some embodiments, a syringe pump 810 for driving a needle 551 to inject or extract liquid is disposed on the table 110, a wide-mouth bottle 820 for containing pure water is disposed on the table 110 corresponding to the syringe pump 810, and the wide-mouth bottle 820 is used for injecting pure water into the pipeline of the syringe pump 810, so as to avoid the influence on the preparation of the sample due to excessive air in the pipeline of the syringe pump 810 during the transfer of the lysate and the matrix.
Referring to fig. 8 and 9, in some of these embodiments, a second scanner 900 is provided on the table 110 for recording the number of plates to facilitate matching of the prepared sample with the relevant microorganism.
In the description of the present specification, if reference is made to the description of "one embodiment", "some embodiments", "exemplary embodiments", "examples", "specific examples", and "some examples", etc., reference is made to the terminology, it is intended that a particular feature, structure, material, or characteristic described in connection with the embodiment or example be included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the utility model have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the utility model, the scope of which is defined by the claims and their equivalents.
Claims (7)
1. A pretreatment device for microbial mass spectrometry detection is characterized by comprising:
the sample preparation device comprises a rack (100), wherein a sample preparation station for preparing a sample is arranged on the rack (100);
the feeding mechanism is arranged on the rack (100), the feeding mechanism can convey a culture dish to a sample preparation station and can separate the cover body and the dish body of the culture dish conveyed to the sample preparation station, the feeding mechanism comprises a storage assembly (200), a feeding assembly (300) and a separating assembly (400), the storage assembly (200) is arranged on the rack (100), the storage assembly (200) is used for placing the culture dish, the feeding assembly (300) is arranged on the rack (100), the feeding assembly (300) can convey the culture dish placed on the storage assembly (200) to the sample preparation station, the separating assembly (400) is arranged on the rack (100), the separating assembly (400) can be matched with the feeding assembly (300) to separate the culture dish body and the dish body of the culture dish conveyed to the sample preparation station, the storage assembly (200) comprises a base (210) for placing a culture dish, the base (210) is arranged on the rack (100) and is located below the sample preparation station, a limiting rod (211) for limiting the circumferential side of the culture dish is arranged on the base (210), the feeding assembly (300) comprises a first driving device (310) and a pushing piece (320), the pushing piece (320) can be arranged on the rack (100) in a vertical sliding manner and is located below the base (210), the first driving device (310) is arranged on the rack (100) and can drive the pushing piece (320) to lift relative to the rack (100), a first avoidance port for avoiding the pushing piece (320) is arranged on the base (210), the limiting rod (211) is arranged on the circumferential side of the first avoidance port, and the separation assembly (400) is arranged on the sample preparation station, the separating assembly (400) comprises a sliding seat (410) and clamping assemblies, the sliding seat (410) is slidably arranged on the rack (100), a second avoidance port (411) for avoiding a culture dish is arranged on the sliding seat (410), two groups of clamping assemblies are arranged on the sliding seat (410), a second driving device (420) is arranged on the rack (100) corresponding to the sliding seat (410), and the second driving device (420) can drive the sliding seat (410) to slide relative to the rack (100) and enable one group of the two groups of clamping assemblies to move to the position right above the pushing piece (320);
the sampling mechanism is arranged at the sample preparation station and can sample microorganisms in a culture dish conveyed to the sample preparation station;
and the sample preparation mechanism is arranged on the sample preparation station and can be matched with the sampling mechanism to prepare samples.
2. The pretreatment apparatus for mass spectrometric detection of microorganisms according to claim 1, wherein said clamping assembly comprises two clamping portions (430) movably disposed on said slide base (410) and a third driving device (440) disposed on said slide base (410) corresponding to said two clamping portions (430), said third driving device (440) being capable of driving said two clamping portions (430) to approach each other or to move away from each other.
3. The pretreatment apparatus for mass spectrometric detection of microorganisms according to claim 2, wherein a buffer member (450) is provided between two of said clamping portions (430) of the same set of said clamping members.
4. The pretreatment apparatus for mass spectrometric detection of microorganisms of claim 1, wherein said sampling mechanism comprises:
the three-axis manipulator (500) comprises an X-axis linear module (510) arranged on the rack (100), a Y-axis linear module (520) arranged on the X-axis linear module (510), and a first Z-axis linear module (530) arranged on the Y-axis linear module (520), wherein a grabbing mechanism is arranged on the first Z-axis linear module (530), and can grab a swab for sampling to sample;
visual positioning subassembly (600), visual positioning subassembly (600) set up in the frame and be located the top of system appearance station, visual positioning subassembly (600) can acquire the distribution information of the bacterial colony of microorganism in the culture dish.
5. The pretreatment apparatus for mass spectrometric detection of microorganisms according to claim 4, wherein said three-axis robot (500) is provided with a laser displacement sensor (540) for detecting the colony height of microorganisms in a culture dish.
6. The pretreatment apparatus for microbial mass spectrometry according to claim 4 or 5, wherein the sample preparation mechanism comprises:
the first placing frame (111), the first placing frame (111) is arranged on the rack (100) and is used for placing a target plate;
the second placing frame (112), the second placing frame (112) is arranged on the rack (100) and is used for placing reagent bottles;
the second Z-axis linear module (550), the second Z-axis linear module (550) is arranged on the Y-axis linear module (520), and a needle (551) capable of injecting liquid or extracting liquid is arranged on the second Z-axis linear module (550).
7. The pretreatment apparatus for mass spectrometric detection of microorganisms according to claim 6, characterized in that a first scanner (700) for recording the number of the target plate is provided on said rack (100) corresponding to said sample preparation mechanism.
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| CN202121521621.6U CN216082592U (en) | 2021-07-05 | 2021-07-05 | Pretreatment device for microbial mass spectrometry detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117451462A (en) * | 2023-12-22 | 2024-01-26 | 珠海迪尔生物工程股份有限公司 | Mass spectrum pretreatment system |
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2021
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117451462A (en) * | 2023-12-22 | 2024-01-26 | 珠海迪尔生物工程股份有限公司 | Mass spectrum pretreatment system |
| CN117451462B (en) * | 2023-12-22 | 2024-04-12 | 珠海迪尔生物工程股份有限公司 | Mass spectrum pretreatment system |
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