CN214991615U - DNA, RNA nucleic acid co-extraction and detecting system - Google Patents

Abstract

The utility model discloses a DNA, RNA nucleic acid co-extraction and detection system, which comprises a nucleic acid extraction system, a sealing transferring and mixing system, a nucleic acid detection system and a control system, wherein the nucleic acid extraction system, the sealing transferring and mixing system and the nucleic acid detection system are all connected with the control system; the nucleic acid extraction system comprises a lifting basket for loading extraction reagent strips and sample tubes, a consumable supply module, a kit loading module, a bar code scanning device for automatically inputting bar code information, a magnetic incubation device and an automatic sample adding device for transferring liquid into a reaction bin for extracting the reagent strips or a PCR tube and sealing the PCR tube; the sealing and transferring system comprises a PCR transferring device and a high-speed mixing device, wherein the PCR transferring device is used for grabbing and driving the PCR tube to move; the nucleic acid detection system comprises a PCR amplification device and a fluorescence detection device, realizes the automation of bar code scanning, sample adding, incubation, transfer mixing and detection, and effectively improves the nucleic acid extraction and amplification efficiency.

Description

DNA, RNA nucleic acid co-extraction and detecting system

Technical Field

The utility model relates to a PCR technical field, more specifically say, relate to a DNA, RNA nucleic acid draw and detecting system altogether.

Background

Due to the characteristics of high sensitivity, high specificity, convenience and quickness of molecular diagnosis, the method is widely applied to clinical diagnosis.

The existing nucleic acid extraction and amplification system mostly adopts a batch sample introduction mode, has few detection items, mostly adopts manual operation in the processes of incubation, magnetic attraction, transfer and the like of a sample to be detected, and mostly adopts manual work to complete sample supply, reagent strip supply and consumable waste in the operation process, so that the working strength of detection personnel is high, fatigue is easy to generate, and the detection efficiency is low, and the detection cost is high.

Meanwhile, each detection process needs to be finished in an independent operation space, detection personnel need to shuttle in a plurality of detection chambers for detection, and the detection efficiency is low.

In summary, how to improve the efficiency of nucleic acid extraction and amplification is an urgent problem to be solved by those skilled in the art.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a DNA and RNA nucleic acid co-extraction and detection system, which realizes the automation of barcode scanning, sample adding, incubation extraction, transfer mixing and detection in the extraction and amplification process, and effectively improves the efficiency of nucleic acid extraction and amplification.

In order to achieve the above object, the present invention provides the following technical solutions:

a DNA and RNA nucleic acid co-extraction and detection system comprises a nucleic acid extraction system, a sealing, transferring and mixing system, a nucleic acid detection system and a control system, wherein the nucleic acid extraction system, the sealing, transferring and mixing system and the nucleic acid detection system are all connected with the control system;

the nucleic acid extraction system comprises a lifting basket for loading extraction reagent strips and a sample tube, a consumable supply module, a kit loading module, a bar code scanning device for automatically inputting bar code information, a magnetic incubation device and an automatic sample adding device for transferring liquid into a reaction bin for extracting the reagent strips or a PCR tube and sealing the PCR tube;

the sealing transfer system comprises a PCR transfer device and a high-speed mixing device, wherein the PCR transfer device is used for grabbing and driving the PCR tube to move;

the nucleic acid detection system comprises a PCR amplification device and a fluorescence detection device.

Preferably, the basket comprises a basket cover plate, a basket bottom plate for containing the reagent strips to be extracted and a sample seat for containing the sample tube, and the sample seat is detachably connected with the basket bottom plate;

the basket cover plate is rotatably connected with the basket bottom plate so as to limit the displacement of the reagent extraction strip in the height direction when the basket cover plate is attached to the basket bottom plate.

Preferably, bar code scanning device includes installing support, scanning subassembly, is used for promoting between scanning the sign indicating number position and the standby position and draws pushing hands subassembly, drive the pushing hands subassembly is followed the pushing hands of the length direction motion of installing support removes subassembly and determine module, determine module is used for judging whether current passageway exists and draws reagent strip and sample tube, determine module with pushing hands removes subassembly signal connection, scanning subassembly detect module all install in on the mounting plate of pushing hands subassembly.

Preferably, the incubation device is inhaled to magnetism includes the incubation module that is used for heating the reaction storehouse, is used for right the reaction storehouse carries out magnetism that inhales and inhale module and drive assembly, the incubation module the magnetism inhale the module all with drive assembly connects, drive assembly is used for the drive the incubation module with the module is inhaled to magnetism and is close to in turn the reaction storehouse.

Preferably, the incubation module includes the heating block that is equipped with the reagent groove, the laminating heating element that the internal face of reagent groove set up, set up in the radiator unit of heating block bottom and be used for detecting the temperature sensor of heating block temperature, temperature sensor with the heating block is connected.

Preferably, the magnetic suction module comprises a magnet and a rotating component for driving the magnet to rotate, and the rotating component is connected with the magnet;

the magnet includes that first magnetism is inhaled face and second magnetism and is inhaled the face, first magnetism inhale the face with the two area variation in size of second magnetism is inhaled the face and is all used for adsorbing the magnetic bead in the reaction bin.

Preferably, the PCR transfer device comprises a gripper assembly for gripping or releasing a PCR tube, an uncovering assembly for opening or closing an amplification cover of the PCR amplification device, an X-axis assembly for driving the gripper assembly and the uncovering assembly to move along an X-axis direction, a Y-axis assembly for driving the gripper assembly and the uncovering assembly to move along a Y-axis direction, and a Z-axis assembly for driving the gripper assembly and the uncovering assembly to move along a Z-axis direction;

the hand grip assembly and the cover opening assembly are respectively connected to the Z shaft assembly in a sliding mode, the Z shaft assembly is connected to the Y shaft assembly in a sliding mode, and the Y shaft assembly is connected to the X shaft assembly in a sliding mode.

Preferably, the hand grip assembly comprises a hand grip base plate, a hand grip movable plate arranged below the hand grip base plate in parallel, a hand grip for gripping the PCR tube and a retraction driving assembly for controlling the hand grip to open or retract, the retraction driving assembly is arranged on the hand grip base plate, and a telescopic end of the retraction driving assembly penetrates through the hand grip base plate to be connected with the hand grip movable plate so as to drive the hand grip movable plate to ascend or descend;

the hand grip base plate and the hand grip movable plate are both connected with the retraction driving assembly, the hand grip is opened when the hand grip movable plate descends, and the hand grip is retracted when the hand grip movable plate ascends.

Preferably, PCR amplification device is including the heating piece that amplifies, amplify heating piece, amplification radiator, be used for the lid to close the amplification lid of the heating piece that amplifies and be used for improving the radiating efficiency's of amplification radiator amplification cooling module, the heating piece that amplifies is equipped with two at least amplification holes that are used for placing the PCR pipe, the lid that amplifies amplify the heating piece that amplifies and the amplification radiator from top to bottom sets gradually.

Preferably, the fluorescence detection device comprises a light source assembly for providing a fluorescence light source, a fluorescence detection assembly for performing quantitative detection, a light source optical fiber and a fluorescence detection optical fiber, wherein one side of the amplification hole is connected with one end of the light source optical fiber, and the other side of the amplification hole is connected with one end of the fluorescence detection optical fiber;

the other end of the light source optical fiber is connected with the light source assembly, and the other end of the fluorescence detection optical fiber is connected with the fluorescence detection assembly.

Preferably, the automatic sample adding device comprises a reagent needle assembly for reagent extraction and injection operation, a sample adding needle assembly for sample liquid extraction and injection operation and a gantry assembly, and the reagent needle assembly, the sample adding needle assembly and the gantry assembly are all connected with the control system;

the reagent needle assembly and the sample injection needle assembly are respectively arranged on two sides of the gantry frame assembly, and the gantry frame assembly can move along the lifting basket, the consumable supply module and the reagent box loading module.

Preferably, the reagent needle assembly comprises a reagent needle, a pricking control assembly, a pipetting control assembly, a reagent needle lifting assembly and a reagent needle translation assembly, the reagent needle comprises an adapter and a reagent pump communicated with the adapter, the reagent pump is connected with the pipetting control assembly, and the adapter is connected with the pricking control assembly, so that the pricking control assembly controls the adapter to prick or withdraw a tip or a sealing plug;

the reagent needle translation assembly is used for driving the reagent needle to move along the horizontal plane and the vertical direction of the movement direction of the gantry frame assembly, and the reagent needle lifting assembly is used for driving the reagent needle to move along the vertical direction.

Preferably, the sample injection needle assembly includes a plurality of liquid transfer pumps arranged in parallel, a liquid transfer pump lifting assembly for driving the liquid transfer pumps to lift, and a plunger lifting assembly for driving plungers of the liquid transfer pumps to slide relative to pump bodies of the liquid transfer pumps, the liquid transfer pumps are mounted on the liquid transfer pump lifting assembly, and the plunger lifting assembly is connected with the plungers of the liquid transfer pumps.

When the DNA and RNA nucleic acid co-extraction and detection system provided by the utility model works, firstly, a sample tube for holding a sample to be detected and a reagent strip are loaded at the position of the lifting basket, and a bar code scanning device is utilized to input the bar code information of the sample tube and the reagent strip; after the recording is finished, the automatic sample adding device punctures the sealing film of the reagent strip and adds the extraction reagent and the sample to be detected into the reaction bin for extracting the reagent strip; the magnetic incubation device performs incubation magnetic attraction treatment on the sample to be detected, and extracts nucleic acid in the sample to be detected; the automatic sample adding device adds an amplification reagent and the purified substances after the incubation magnetic attraction into the PCR tube, and seals the PCR tube by using a sealing plug; after sealing, the PCR transfer device grabs the PCR tube and transfers the PCR tube to the high-speed mixing device; after the mixing is finished, the PCR transfer device transfers the PCR tube into the PCR amplification device to finish the PCR amplification process; and after the amplification process is finished, carrying out fluorescence detection on the PCR tube by using the fluorescence detection device.

Therefore, the utility model provides a DNA, RNA draw and amplification system has realized in the testing process bar code scanning, application of sample, incubation extraction, the automation of transferring mixing and fluorescence detection, has improved nucleic acid extraction amplification efficiency effectively.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an embodiment of a DNA/RNA nucleic acid co-extraction and detection system provided by the present invention; FIG. 2 is a schematic structural view of the basket; FIG. 3 is an exploded view of the basket; FIG. 4 is a schematic structural view of a buckle assembly on a workbench; FIG. 5 is a schematic view of the pressing assembly on the worktable; FIG. 6 is a schematic structural view of a disposable consumable tip cartridge; FIG. 7 is a schematic structural view of a PCR consumable cassette; FIG. 8 is a schematic view of a barcode scanning device; FIG. 9 is a schematic view of a scanning assembly; FIG. 10 is a schematic structural view of the handle assembly; FIG. 11 is a schematic view of the magnetic incubation device; FIG. 12 is a schematic structural view of an incubation module; FIG. 13 is a schematic cross-sectional view of FIG. 12; FIG. 14 is a schematic view of a magnetic module; FIG. 15 is a schematic view showing the assembly of the magnet, the magnet holder and the magnetic shield; FIG. 16 is a schematic view of the structure of the magnet; FIG. 17 is a schematic structural view of an automatic sample adding device; FIG. 18 is a schematic view of the structure of the automatic sample adding device in another direction; FIG. 19 is a schematic view of the construction of the reagent needle assembly; FIG. 20 is a schematic view of the construction of a needle loading assembly; FIG. 21 is a schematic view of the construction of a PCR transfer apparatus; FIG. 22 is a schematic structural view of a Z-axis assembly; FIG. 23 is a schematic view of a gripper assembly; FIG. 24 is an exploded view of a PCR amplification apparatus; FIG. 25 is a schematic view of the structure of the fluorescence detection device.

In fig. 1-25:

01 is tip; 021 is PCR tube, 022 is sealing plug; 03 is an extraction reagent strip and 031 is a reaction bin; 04 is a sample tube; 11 is a basket, 111 is a basket bottom plate, 112 is a basket cover plate, 113 is a sample seat, 114 is a supporting component, 115 is a handle, and 116 is a basket positioning component; 12 is a disposable tip consumable box; 13 is a PCR consumable box; 14 is a kit loading module; 15 is a bar code scanning device, 151 is a mounting bracket, 152 is a pushing handle moving component, 153 is a scanning component, 1531 is a reflector, 1532 is a code reader, 154 is a pushing handle component, 155 is a detection component; 16 is a magnetic attraction incubation device, 161 is an incubation module, 162 is a magnetic attraction module, 163 is a driving component, 164 is a limit supporting component, 1611 is a heating block, 1611-1 is a reagent tank, 1612 is a heating component, 1613 is a heat dissipation component, 1614 is a temperature sensor, 1615 is a heat preservation component, 1616 is a cooling component, 1621 is a magnet, 1621-1 is a first magnetic attraction surface, 1621-2 is a second magnetic attraction surface, 1622 is a rotating component, and 1623 is a magnetic isolation plate; 17 is an automatic sample adding device, 171 is a reagent needle assembly, 172 is a sample adding needle assembly, 173 is a gantry frame assembly, 1711 is an adapter, 1711-1 is a sleeve, 1711-2 is an adapter mounting plate, 1711-3 is a sleeve mounting plate, 1712 is a reagent pump, 1713 is a pricking control assembly, 1713-1 is a guide pillar, 1713-2 is a guide pillar connecting plate, 1713-3 is a screw motor, 1714 is a reagent needle translation assembly, 1715 is a tip taking detection assembly, 1716 is a tip withdrawing detection assembly, 1721 is a liquid transferring pump, 1722 is a plunger lifting assembly, 1723 is a liquid transferring pump lifting assembly, 1724 is a liquid drawing and injecting detection assembly, and 1725 is a Z-axis origin detection assembly; 18 is a liquid-pocket air draft device, 191 is a buckle component and 192 is a pressing component; 21 is a PCR transfer device, 211 is a gripper assembly, 212 is an uncovering assembly, 213 is an X shaft assembly, 214 is a Y shaft assembly, 215 is a Z shaft assembly, and 22 is a high-speed blending device; 31 is a PCR amplification device, 311 is an amplification heating block, 312 is an amplification heating sheet, 313 is an amplification heat sink, 314 is an amplification cover, 315 is an amplification cooling component, 32 is a fluorescence detection device, 321 is a light source component, 322 is a fluorescence detection component, 323 is a light source fiber, and 324 is a fluorescence detection fiber.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The core of the utility model is to provide a DNA, RNA nucleic acid draw and detecting system altogether, realized extracting bar code scanning, application of sample, incubation among the amplification process and draw, shift the automation of even and detection, improved nucleic acid extraction amplification efficiency effectively.

Please refer to fig. 1-25. It should be noted that, in the present application, the X-axis direction is a moving direction of the gantry assembly 173 of the automatic sample adding device 17, the Y-axis direction is a direction perpendicular to the X-axis direction in a horizontal plane, and the Z-axis direction is a vertical direction.

The utility model provides a DNA, RNA nucleic acid co-extraction and detection system, which comprises a nucleic acid extraction system, a sealing, transferring and mixing system and a nucleic acid detection system; the nucleic acid extraction system comprises a basket 11 for loading the extraction reagent strip 03 and the sample tube 04, a consumable supply module, a kit loading module 14, a bar code scanning device 15 for automatically inputting bar code information, a magnetic incubation device 16 and an automatic sample adding device 17 for adding liquid into a reaction bin 031 for extracting the reagent strip 03 or a PCR tube 021 and sealing the PCR tube 021; the sealing and transferring system comprises a PCR transfer device 21 and a high-speed blending device 22, wherein the PCR transfer device 21 is used for grabbing and driving the PCR tube 021 to move; the nucleic acid detection system includes a PCR amplification device 31 and a fluorescence detection device 32.

Wherein, the consumptive material supply module is used for holding the required disposable consumptive material in the testing process, like tip01 and PCR pipe 021, and the consumptive material is got through automatic application of sample device 17 and PCR transfer device 21 and is put in the testing process, need not the testing personnel manual operation, has alleviateed testing personnel's working strength effectively.

Referring to fig. 1, the consumable supply module includes a disposable tip consumable cassette 12 for containing tip01 and a PCR consumable cassette 13 for containing PCR tubes 021 and their corresponding sealing plugs 022. The structure, material, size, position, etc. of the disposable tip consumable cassette 12 and the PCR consumable cassette 13 are determined according to actual detection requirements.

The reagent kit loading module 14 is used for placing a plurality of amplification reagents required by the construction of a PCR system, and the structure, material, size, position and the like of the reagent kit loading module 14 are determined according to actual detection requirements.

Before a sample to be detected is detected, a sample tube 04 for containing the sample to be detected and an extraction reagent strip 03 are loaded at a lifting basket 11, and bar code information of the sample tube 04 and the extraction reagent strip 03 is input by using a bar code scanning device 15; during detection, the lifting basket 11 is fixed on the workbench, the automatic sample adding device 17 punctures a sealing film of the extracted reagent strip 03, and gradually adds the extracted reagent and sample liquid of a sample to be detected into a reaction bin 031 for extracting the reagent strip 03; after the sample is added, the magnetic incubation device 16 performs incubation magnetic incubation treatment on the mixed solution to extract nucleic acid in the mixed solution; after extraction, the automatic sample adding device 17 gradually adds amplification reagents and incubates the magnetically attracted purified product into the PCR tube 021, and seals the PCR tube 021 with a sealing plug 022 after liquid transfer is completed; after sealing, the PCR transfer device 21 grabs the PCR tube 021 and transfers the PCR tube 021 to the high-speed mixing device 22 for high-speed mixing; after the mixing is finished, the PCR transfer device 21 transfers the PCR tube 021 into the PCR amplification device 31 to complete the PCR amplification process; after the amplification process is finished, the fluorescence detection device 32 performs fluorescence detection on the PCR tube 021.

Therefore, the DNA and RNA co-extraction and amplification system provided by the embodiment realizes the automation of bar code scanning, sample adding, incubation extraction, transfer mixing and fluorescence detection in the detection process, and effectively improves the nucleic acid extraction and amplification efficiency.

Preferably, the nucleic acid extraction system is further provided with a pocket liquid air draft device 18, and the pocket liquid air draft device 18 comprises a pocket liquid box for collecting the reagent dropped in the liquid to be pipetted and an air draft device for conducting air draft treatment on the liquid level to be pumped and injected. The liquid pocket box can effectively prevent the reagent from dropping on the operation plane to cause cross contamination; the air draft device can draw out the polluted gas generated by pumping and injecting liquid, and the pollution caused by aerosol diffusion is avoided.

On the basis of the above embodiment, the basket 11 includes a basket cover plate 112, a basket bottom plate 111 for containing the reagent strips 03 to be extracted, and a sample holder 113 for containing the sample tube 04, and the sample holder 113 is detachably connected to the basket bottom plate 111; the basket cover 112 is rotatably connected to the basket base 111 to limit the displacement of the extracted reagent strip 03 in the height direction when the basket cover 112 is attached to the basket base 111.

Referring to fig. 2, the center of the bottom plate 111 of the basket is provided with a plurality of reagent strip mounting holes arranged in parallel along the length direction thereof, and the sample holder 113 is provided with a plurality of sample tube mounting holes; the sizes and the number of the reagent strip mounting holes and the sample tube mounting holes are determined according to actual detection requirements.

The basket cover plate 112 is rotatably connected with the basket bottom plate 111 to prevent the reagent strips 03 from moving in the height direction of the basket 11, and preferably, the basket cover plate 112 is rotatably connected with one end of the basket bottom plate 111 in the length direction; reagent strip positioning holes corresponding to the reagent strip mounting holes one to one are arranged on the basket cover plate 112.

Preferably, the top surface of the basket bottom plate 111 is provided with a first magnet, the bottom plate of the basket cover plate 112 is provided with a second magnet, and when the basket cover plate 112 is attached to the basket bottom plate 111, the first magnet and the second magnet attract each other. Therefore, draw reagent strip 03 the perk or need overcome the attraction between first magnet and the second magnet when deviating from, realized drawing reagent strip 03 effective spacing and accurate positioning in the basket 11 direction of height. In addition, the first magnet and the second magnet can be replaced by positioning grooves, positioning blocks and other similar positioning structures which are clamped with each other.

In order to facilitate loading of the reagent strips 03 and the sample tubes 04, preferably, the bottom surface of the basket bottom plate 111 is provided with a support assembly 114, and the support assembly 114 may be an i-shaped structure shown in fig. 2, may also be support columns arranged at four corners of the basket bottom plate 111, and may also be provided with other common support structures.

When the extraction reagent strips 03 and the sample tubes 04 are loaded, the basket cover plate 112 is rotated, the extraction reagent strips 03 and the sample tubes 04 are respectively placed in the basket bottom plate 111 and the sample seats 113, and after the placement is finished, the basket cover plate 112 is rotated to enable the basket bottom plate 111 to be attached to the extraction reagent strips 03 and the sample tubes 04; after loading, placing the lifting basket 11 on a workbench and fixing; after the test is completed, the basket 11 is removed from the table, and the used reagent strip 03 and sample tube 04 are discarded.

In the embodiment, batch synchronous loading of the extraction reagent strips 03 and the sample tubes 04 is realized, a tedious process of placing for multiple times is saved, and the placing efficiency is high; the basket 11 can accurately position the placed reagent strips 03 and the sample tubes 04, and the phenomenon that the basket 11 is repositioned after being placed on the workbench is avoided.

In order to fix the basket 11, the workbench is provided with a buckle assembly 191 and a pressing assembly 192 for positioning the basket 11, the buckle assembly 191 and the pressing assembly 192 are respectively clamped with two ends of the basket 11 in the length direction, and the structures, sizes, materials, quantities, setting positions and the like of the buckle assembly 191 and the pressing assembly 192 are determined according to actual production needs and are not described herein again.

Preferably, the basket bottom plate 111 is rotatably connected to the handle 115 to facilitate the transportation of the basket 11 and improve the transportation efficiency, and the structure, shape, size, number, material, position, etc. of the handle 115 are determined according to actual needs.

Preferably, the basket 11 further comprises a basket positioning assembly 116, the basket positioning assembly 116 comprises a guide seat, an extension spring, a guide shaft arranged along the length direction of the basket bottom plate 111 and a basket baffle plate used for limiting the extraction of the reagent strips 03, and the guide seat is connected to the bottom surface of the basket bottom plate 111; the guide shaft is connected with the guide seat in a sliding mode, one end of the guide shaft is vertically connected with the basket baffle, and two ends of the extension spring are connected with the basket baffle and the bottom surface of the basket bottom plate 111 respectively.

Referring to fig. 3, one end of the basket bottom plate 111 close to the rotation axis of the basket cover plate 112 is the front end of the basket bottom plate 111. When reagent strips 03 are loaded and extracted, the tension spring is pulled manually to drive the lifting basket baffle plate to move towards the front end of the lifting basket bottom plate 111, the lifting basket baffle plate is located on the right side of the reagent strips 03, therefore, the lifting basket baffle plate pushes the reagent strips 03 to move towards the front end of the lifting basket bottom plate 111 until the reagent strips 03 are in contact with the left wall surface of the reagent strip mounting hole, and the placing positions of the reagent strips 03 are consistent.

On the basis of the above embodiment, the barcode scanning device 15 includes a mounting bracket 151, a scanning component 153, a pushing component 154 for pushing the extracted reagent strip 03 between the code scanning position and the standby position, a pushing moving component 152 for driving the pushing component 154 to move along the length direction of the mounting bracket 151, and a detection component 155, where the detection component 155 is used to determine whether the extracted reagent strip 03 and the sample tube 04 exist in the current channel, the detection component 155 is in signal connection with the pushing moving component 152, and both the scanning component 153 and the detection component 155 are installed on the installation bottom plate of the pushing component 154.

The pushing arm moving assembly 152 includes a linear displacement mechanism and a driving mechanism, the linear displacement mechanism is disposed along the length direction of the mounting bracket 151, and specifically, the linear displacement mechanism may be a combination of a common linear displacement structure such as a synchronous belt mechanism and a guide rail slider mechanism, and a driving mechanism such as a stepping motor, an electric push rod, and a hydraulic cylinder.

Referring to fig. 9, the scanning assembly 153 includes a reflector 1531 and a code reader 1532, the reflector 1531 is used for reflecting the detection light of the code reader 1532 to the barcode of the sample tube 04, so as to realize the code scanning and recording of the sample tube 04 by the code reader 1532. The type and size of the code reader 1532, the type and size of the mirror 1531, and the relative position relationship between the mirror 1531 and the code reader 1532 are determined according to actual needs by referring to the prior art, and are not described herein again.

The pushing assembly 154 comprises a linear displacement mechanism, a driving mechanism and a pushing handle for pushing the reagent strips 03 to move, and the pushing handle is connected to the linear displacement mechanism; for the specific arrangement of the linear displacement mechanism and the driving mechanism, please refer to the above-mentioned pushing handle moving assembly 152.

Preferably, referring to fig. 10, the mounting bottom plate of the pushing handle assembly 154 is provided with a pushing block for being clamped with the reagent strip 03, a wire rail, a synchronous belt mechanism, and a stepping motor for driving the synchronous belt mechanism, the pushing block is slidably connected with the wire rail, and the length direction of the wire rail is perpendicular to the length direction of the mounting bracket 151. When the stepping motor rotates, the synchronous belt mechanism is driven to drive the push block to slide relative to the linear rail, and the push block is contacted with the extraction reagent strip 03, so that the extraction reagent strip 03 moves between the standby position and the code scanning position.

The detection assembly 155 comprises a reagent strip detection sensor for detecting whether the extracted reagent strip 03 exists in the current channel and a sample tube detection sensor for detecting whether the sample tube 04 exists in the current channel, and the reagent strip detection sensor is in signal connection with the pushing assembly 154.

When the reagent strip detection sensor detects that the reagent strip 03 is extracted, the pushing hand assembly 154 drives the reagent strip 03 to move from the standby position to the code scanning position; when the sample tube detection sensor detects the sample tube 04, the scanning component 153 scans the barcode information of the sample tube 04; when neither of the two detects the barcode information, the pushing moving assembly 152 drives the pushing assembly 154 to move to the next channel.

During operation, the detection assembly 155 is used for judging whether the extracted reagent strip 03 and the sample tube 04 exist in the current channel or not, if yes, the pushing assembly 154 is used for driving the extracted reagent strip 03 to move to a code scanning position, the scanning assembly 153 is used for scanning the two-dimensional code of the extracted reagent strip 03 and/or the sample tube 04, after scanning is completed, the pushing assembly 154 is used for driving the extracted reagent strip 03 to return to a standby position, and the pushing movement assembly 152 is used for driving the pushing assembly 154 and the scanning assembly 153 to move to the next channel; if not, the pushing arm moving assembly 152 directly drives the pushing arm assembly 154 and the scanning assembly 153 to move to the next channel.

In this embodiment, the scanning assembly 153 and the pushing assembly 154 can move along the length direction of the mounting bracket 151, and can read multi-channel bar code information; the scanning component 153 can scan the extracted reagent strip 03 and can also scan the sample tube 04, and the detection speed is high; meanwhile, the barcode scanning device 15 has a compact structure, can operate in a narrow and long space, and is beneficial to reducing the machine size of a DNA and RNA nucleic acid co-extraction and detection system and improving the space utilization rate.

Preferably, the detection assembly 155 further includes a pusher in-position sensor for detecting whether the extracted reagent strip 03 reaches the code scanning position and a pusher reset sensor for detecting whether the extracted reagent strip 03 returns to the standby position, and both the pusher in-position sensor and the pusher reset sensor are in signal connection with the pusher assembly 154. The handle-pushing in-place sensor and the handle-pushing in-place sensor can be set as a proximity switch, a travel switch and the like.

When the pushing assembly 154 moves to the last channel of the mounting bracket 151, the pushing moving assembly 152 can be controlled to drive the pushing assembly 154 to reset, and the extracted reagent strips 03 and the sample tubes 04 in each channel are sequentially detected from the first channel; the pushing handle moving assembly 152 can also be controlled to drive the scanning assembly 153 to scan the next set of extracted reagent strips 03 and sample tubes 04 to be detected in the reverse order.

Preferably, in order to reset the scanning assembly 153, the detecting assembly 155 may further include a scanning reset sensor for detecting whether the scanning assembly 153 is moved to the end of the mounting bracket 151, and the scanning reset sensor is in signal connection with the pusher moving assembly 152.

On the basis of the above embodiment, the magnetic attraction incubation device 16 includes an incubation module 161 for heating the reaction chamber 031, a magnetic attraction module 162 for magnetically attracting the reaction chamber 031, and a driving component 163, wherein the incubation module 161 and the magnetic attraction module 162 are both connected to the driving component 163, and the driving component 163 is used for driving the incubation module 161 and the magnetic attraction module 162 to alternately approach the reaction chamber 031.

After the automatic sample adding device 17 adds the sample liquid of each extraction reagent and the sample to be tested into the reaction bin 031, the driving component 163 is controlled to make the incubation module 161 close to the reaction bin 031, so as to effectively heat the mixed liquid in the reaction bin 031 and facilitate the incubation operation of the mixed liquid, and meanwhile, the magnetic absorption module 162 is far away from the reaction bin 031 to avoid affecting the incubation process; after the incubation process is completed, the driving assembly 163 is controlled to make the magnetic attraction module 162 approach to the reaction bin 031, so that the magnetic beads in the mixed solution are adsorbed to the inner wall surface of the reaction bin 031, and meanwhile, the incubation module 161 is far away from the reaction bin 031; after the magnetic attraction operation is completed, the driving assembly 163 is controlled to make the magnetic attraction module 162 far away from the reaction bin 031.

In this embodiment, the driving component 163 is used to alternately control the incubation module 161 and the magnetic attraction module 162 to be close to the reaction bin 031, so that the incubation module 161 is contacted and separated from the reaction bin 031, the situation that the temperature of the mixed liquid is too slow when the incubation module 161 heats the mixed liquid in the reaction bin 031 all the time is avoided, and the operation efficiency of the device is improved; meanwhile, the integral arrangement of the incubation module 161 and the magnetic attraction module 162 effectively reduces the overall size of the device and simplifies the incubation and magnetic attraction operation steps.

The driving component 163 can be set as a screw driving mechanism, a rail driving mechanism and other driving mechanisms, preferably, the driving component 163 is a screw driving mechanism, the screw driving mechanism can be vertically set as shown in fig. 11, and can also be horizontally set according to the overall layout of the incubation magnetic attraction device 16, as long as the driving component 163 can drive the incubation module 161 to lift.

Preferably, the magnetic incubation device 16 may further include a limit support component 164, the magnetic module 162 and the driving component 163 are both connected to the limit support component 164, and the limit support component 164 is provided with a limit track for limiting the traversing track of the magnetic module 162; when the driving assembly 163 drives the incubation module 161 to ascend, the magnetic attraction module 162 is far away from the reaction bin 031 along the limiting track, and when the driving assembly 163 drives the incubation module 161 to descend, the magnetic attraction module 162 is close to the reaction bin 031 along the limiting track.

Referring to fig. 11-14, preferably, the position-limiting support assembly 164 includes a horizontal support plate and a vertical support plate, which are vertically connected; the horizontal support plate is provided with a limit track, and the magnetic suction module 162 is connected with the limit track in a sliding manner; a synchronous belt mechanism connected with the magnetic module 162 is arranged on the top of the vertical support plate along the vertical direction; the synchronous belt mechanism is provided with a blocking piece, and the telescopic end of the driving component 163 is provided with a power sheet used for being abutted against the blocking piece.

When the telescopic end of the driving component 163 drives the incubation module 161 to descend, the power sheet is clamped with the blocking piece, so that the power sheet can drive the blocking piece to move downwards, and further the synchronous belt of the synchronous belt mechanism moves downwards and tensions the synchronous belt, so that the magnetic attraction module 162 is close to the vertical support plate along the limiting track and close to the close reaction bin 031; on the contrary, when the telescopic end of the driving component 163 drives the incubation module 161 to ascend, the power sheet moves upward and separates from the blocking sheet, and drives the magnetic attraction module 162 to be far away from the reaction bin 031; therefore, the lifting movement of the incubation module 161 and the movement of the magnetic attraction module 162 close to or far from the reaction bin 031 are controlled by the same driving component 163, and the overall structure of the magnetic attraction incubation device 16 is simplified.

In order to facilitate the resetting of the magnetic attraction module 162, a magnetic attraction return spring may be disposed on the horizontal support plate, and two ends of the magnetic attraction return spring are respectively connected to the vertical support plate and the magnetic attraction module 162; or set up the magnetism that drive magnetism inhale module 162 and remove the subassembly along spacing subassembly removal, magnetism inhale module 162 and install in magnetism and inhale the removal subassembly, magnetism inhale the removal subassembly and specifically can include linear displacement mechanism such as linear guide mechanism and actuating mechanism such as lead screw motor, pneumatic cylinder.

On the basis of the above embodiment, the incubation module 161 includes a heating block 1611 having a reagent well 1611-1, a heating unit 1612 attached to an inner wall surface of the reagent well 1611-1, a heat radiating unit 1613 disposed at a bottom of the heating block 1611, and a temperature sensor 1614 for detecting a temperature of the heating block 1611, and the temperature sensor 1614 is connected to the heating block 1611.

In order to realize the simultaneous incubation of a plurality of reaction bins 031, a plurality of reagent grooves 1611-1 are arranged in the heating block 1611, so that the incubation temperatures of the plurality of reaction bins 031 are the same during heating, the problem of temperature uniformity is solved, the accuracy of detection results is improved, and experimental errors are reduced. The specific number of reagent wells 1611-1 is determined according to actual needs and will not be described herein.

Preferably, the distribution of the heating power of the heating unit 1612 on the inner wall surface of the reagent vessel 1611-1 is non-uniform distribution to ensure the temperature uniformity at each position inside the heating block 1611.

Preferably, a heat-retaining unit 1615 is disposed at the outer periphery of the heating block 1611, and a cooling unit 1616 is disposed below the heat-dissipating unit 1613, so as to improve heat-dissipating efficiency and facilitate arbitrary and rapid switching of the incubation temperature within the incubation temperature range.

The structures, materials, dimensions, positions, connection manners, etc. of the heating block 1611, the heating element 1612, the heat dissipation element 1613, the temperature sensor 1614, the heat preservation element 1615, and the cooling element 1616 are determined according to the actual incubation requirement with reference to the prior art, and are not described herein again.

The incubation module 161 is operated by first heating the heating block 1611 by the heating assembly 1612, stopping the heating operation of the heating assembly 1612 when the temperature sensor 1614 detects that the temperature of the heating block 1611 reaches a first preset temperature (usually set to 37 ℃), and keeping the temperature of the heating block 1611 constant by the temperature keeping assembly 1615, so as to incubate the sample liquid in the reaction chamber 031.

When the first incubation is finished and the magnetic attraction is completed, the heating block 1611 is rapidly heated again by the heating unit 1612, and when the temperature sensor 1614 detects that the temperature of the heating block 1611 reaches the second preset temperature (usually set to 80 ℃), the heating operation of the heating unit 1612 is stopped, and the incubation unit 1615 is turned on to keep the temperature of the heating block 1611 constant, so as to re-incubate the sample solution.

After the temperature raising of the sample solution is finished, the heat dissipation assembly 1613 is utilized to cool the heating block 1611, and the cooling assembly 1616 is turned on to improve the heat dissipation efficiency of the heat dissipation assembly 1613.

In this embodiment, the heating unit 1612 is attached to the inner wall surface of the reagent vessel 1611-1 so that the temperature of the reagent vessel 1611-1 is rapidly increased; cooling assembly 1616 increases the heat dissipation efficiency of heat dissipation assembly 1613, such that the temperature of reagent well 1611-1 is rapidly decreased, thereby achieving a rapid temperature transition and increasing the incubation efficiency.

On the basis of the above embodiment, the magnetic module 162 includes a magnet 1621 and a rotating component 1622 for driving the magnet 1621 to rotate, and the rotating component 1622 is connected to the magnet 1621; the magnet 1621 includes a first magnetic attraction surface 1621-1 and a second magnetic attraction surface 1621-2, the first magnetic attraction surface 1621-1 and the second magnetic attraction surface 1621-2 have different areas and are used for adsorbing magnetic beads in the reaction chamber 031.

A plurality of magnets 1621 are arranged in parallel with each other, and the number of the magnets 1621 is the same as that of the reagent wells 1611-1 in the incubation module 161; the magnetic attraction surfaces of the first magnetic attraction surface 1621-1 and the second magnetic attraction surface 1621-2 are different in area, so that the magnetic force of the magnetic attraction module 162 can be changed by switching the first magnetic attraction surface 1621-1 and the second magnetic attraction surface 1621-2.

Referring to fig. 17-18, the area of the first magnetic attraction surface 1621-1 is larger than that of the second magnetic attraction surface 1621-2, so that when the reaction chamber 031 requires a large magnetic force, the rotating assembly 1622 is controlled to rotate the first magnetic attraction surface 1621-1 to a matching surface facing the reaction chamber 031; on the contrary, when a smaller magnetic force is required, the rotating assembly 1622 is controlled to rotate the second magnetic attraction surface 1621-2 to the mating surface facing the reaction chamber 031.

When the magnetic module 162 works, the magnetic surface facing the reaction chamber 031 is determined according to the required magnetic force, and the rotating component 1622 is controlled to make the magnetic surface facing the mating surface of the reaction chamber 031. When the magnetic attraction face needs to be changed, the magnetic attraction module 162 is controlled to be away from the reaction bin 031, then the rotating assembly 1622 is controlled to rotate to enable the other magnetic attraction face to rotate to the reaction bin 031, and after the adjustment is completed, the magnetic attraction module 162 is controlled to be close to the reaction bin 031 and enable the other magnetic attraction face to be attached to the matching face of the reaction bin 031.

In this embodiment, the rotating assembly 1622 realizes the magnetic force transformation through the magnetic attraction surface, and has simple structure, convenient operation and convenient popularization.

Preferably, in order to avoid the interference of the magnet 1621 to the magnetic bead suspension of the reaction chamber 031 under the non-magnetic attraction state, the magnetic attraction module 162 is provided with a magnetic isolation plate 1623 for isolating the magnetic bead in the non-magnetic attraction process, and the shape, size, material, connection mode, connection position, and the like of the magnetic isolation plate 1623 are determined according to actual detection requirements, and are not described herein again.

Preferably, the rotating assembly 1622 includes a timing belt mechanism for driving the rotating shaft of the magnetic attraction module 162 and a rotating motor for driving the timing belt mechanism. The shapes, structures, sizes, materials, positions and the like of the rotating motor, the synchronous belt mechanism and the rotating shaft are determined according to actual detection requirements, and are not described in detail herein. When the rotating motor works, the synchronous belt mechanism is driven to rotate, so that the rotating shaft of the magnetic module 162 is driven to rotate, when the rotating shaft rotates, the magnet 1621 rotates circumferentially, the first magnetic attraction surface 1621-1, the second magnetic attraction surface 1621-2 and the magnetic isolation plate 1623 are switched, and then magnetic force switching and magnetic isolation operation are realized.

Alternatively, the rotary shaft may be directly driven by a rotary motor.

On the basis of the above embodiments, the automatic sample adding device 17 includes a reagent needle assembly 171 for reagent extraction and injection operation, a sample adding needle assembly 172 for sample liquid extraction and injection operation, and a gantry assembly 173, wherein the reagent needle assembly 171, the sample adding needle assembly 172, and the gantry assembly 173 are all connected to the control system; the reagent needle assembly 171 and the sample injection needle assembly 172 are respectively disposed at both sides of the gantry assembly 173, and the gantry assembly 173 can move along the basket 11, the consumable supply module, and the cartridge loading module 14.

The reagent needle assembly 171 is responsible for extracting and injecting reagents, the sample injection needle assembly 172 is responsible for adding and uniformly mixing sample liquid, and the gantry assembly 173 is responsible for transferring and positioning the reagent needle assembly 171 and the sample injection needle assembly 172 so as to realize multichannel synchronous operation and improve the detection efficiency of the device.

Referring to fig. 17, the carrier 11, the consumable supply module, and the reagent cassette loading module 14 are disposed in parallel on a table at the upper end of the gantry 173.

When the automatic sample adding device 17 works, firstly, the control system controls the portal frame assembly 173 to drive the sample adding needle assembly 172 to move to the area of the lifting basket 11 loaded with the extracted reagent strips 03, and the sample adding needle assembly 172 pricks tip01 and punctures the aluminum plastic film on the top of the extracted reagent strips 03 through hole sites one by one; the portal frame assembly 173 drives the reagent needle assembly 171 to move above the disposable tip consumable box 12, the reagent needle assembly 171 pricks the tip01, the portal frame assembly 173 drives the reagent needle assembly 171 to move above the reagent box loading module 14, the reagent needle assembly 171 extracts the extracted reagent, the portal frame assembly 173 drives the reagent needle assembly 171 to move above the extracted reagent strip 03, the reagent needle assembly 171 fills the extracted reagent into the reaction bin 031 of the extracted reagent strip 03, after filling, the portal frame assembly 173 drives the reagent needle assembly 171 to move above a waste hole site, and the reagent needle assembly 171 withdraws from the tip01 and discards the reagent needle assembly 171; repeating the steps to complete the sample adding process of all the extracted reagents; the gantry assembly 173 drives the sample injection needle assembly 172 to extract the sample liquid in the sample tube 04 loaded by the basket 11 and inject the sample liquid into the reaction bin 031 of the extraction reagent strip 03, and the mixed liquid in the reaction bin 031 is subjected to liquid pumping and injecting operations for multiple times, so that the extraction reagent and the sample liquid are fully mixed; after mixing is completed, the gantry assembly 173 drives the loading needle assembly 172 away from the extraction reagent strip 03 to facilitate the subsequent incubation magnetic attraction process.

In the magnetic suction process, the control system controls the gantry assembly 173 to drive the sample injection needle assembly 172 to extract all liquid in the reaction bin 031 and transfer the liquid to the reagent hole for extracting the reagent strip 03; the gantry frame assembly 173 drives the sample feeding needle assembly 172 to draw and feed the washing solution in the reagent strip 03 into the reaction bin 031, at this time, the magnetic suction module 162 is far away from the reaction bin 031, and the washing solution washes away the magnetic beads aggregated on the inner wall surface of the reaction bin 031 in the feeding process; after the filling, the sample injection needle assembly 172 performs a plurality of operations for extracting the liquid so that the magnetic beads and the washing solution are sufficiently mixed.

In the magnetic incubation process, since the reagent needle assembly 171 is in an idle period, it is preferable to construct a PCR system using the reagent needle assembly 171 in order to increase the detection speed and reduce the detection time. First, the gantry assembly 173 drives the reagent needle assembly 171 to move above the reagent cartridge loading module 14, extracts the amplification reagents and fills them into the PCR tube 021 located in the PCR consumable cartridge 13, and discards the tip01 after the filling is completed; and repeating the steps to complete the filling process of all the amplification reagents.

After the incubation magnetic attraction process is finished, the magnetic attraction module 162 is kept still and still attached to the reaction bin 031, the control system controls the portal frame assembly 173 to drive the reagent needle assembly 171 to move to the position above the area where the extraction reagent strips 03 are loaded on the lifting basket 11, and the purified substances in the reaction bin 031 are extracted and filled into the PCR tube 021 containing the amplification reagents; then, the portal frame assembly 173 drives the reagent needle assembly 171 to suck and extract the sealing liquid in the reagent strip 03 and fill the sealing liquid into the PCR tube 021; finally, the gantry assembly 173 drives the reagent needle assembly 171 to move above the PCR consumable box 13, pricks the sealing plug 022 and then moves above the PCR tube 021, and seals the PCR tube 021 by the sealing plug 022.

In the above-described procedure, when the kind of liquid sucked or injected by the reagent needle assembly 171 and the injection needle assembly 172 is changed, tip01 used before the replacement needs to be discarded to prevent contamination of the reagent during the liquid injection.

In the embodiment, the full-automatic extraction and addition of the sample liquid and the reagent are realized, the labor intensity of detection personnel is effectively reduced, and the detection efficiency and the operation accuracy are improved.

Preferably, the gantry assembly 173 includes a gantry and a sample-adding moving assembly for driving the gantry to move; the workstation is equipped with the application of sample direction subassembly of restriction portal frame moving direction, application of sample direction subassembly and portal frame sliding connection, and application of sample moving assembly is connected with control system.

Referring to fig. 17, the reagent needle assembly 171 and the sample injection needle assembly 172 are respectively disposed at two sides of the gantry, and when the sample injection moving assembly drives the gantry to move along the extending direction of the sample injection guide assembly, the gantry drives the reagent needle assembly 171 and the sample injection needle assembly 172 to move along the lifting basket 11, the consumable supply module, and the reagent cartridge loading module 14.

The application of sample direction subassembly can set up to common linear displacement mechanism such as guide rail slider mechanism, guide pin bushing guide pillar mechanism or rack and pinion, and application of sample removes actuating mechanism such as subassembly then can select driving motor, pneumatic cylinder, electric putter, and application of sample direction subassembly and application of sample remove the structure, size, position etc. of subassembly and need consult prior art according to actual detection and confirm, no longer give unnecessary details here.

On the basis of the above embodiment, the reagent needle assembly 171 includes a reagent needle, a pricking control assembly 1713, a pipetting control assembly, a reagent needle lifting assembly and a reagent needle translation assembly 1714, the reagent needle includes an adapter 1711 and a reagent pump 1712 communicated with the adapter 1711, the reagent pump 1712 is connected with the pipetting control assembly, the adapter 1711 is connected with the pricking control assembly 1713, so that the pricking control assembly 1713 controls the adapter 1711 to prick or withdraw the tip01/PCR tube 021; the reagent needle translation assembly 1714 is configured to drive the reagent needle to move in a direction perpendicular to the direction of movement of the gantry assembly 173 in the horizontal plane, and the reagent needle elevation assembly is configured to drive the reagent needle to move in the vertical direction.

Referring to fig. 18 and 19, a reagent needle mounting seat is provided in the reagent needle assembly 171, the reagent needle mounting seat is used for mounting a reagent needle, a pricking control assembly 1713, a pipetting control assembly and a reagent needle lifting assembly, and the reagent needle mounting seat is slidably connected to the gantry assembly 173 through a reagent needle translation assembly 1714.

Adapter 1711 is used for matching connection with tip01 or sealing plug 022; the reagent pump 1712 is a liquid suction pump for connecting and communicating with the adapter 1711, and is connected to the movement control unit. The type and size of the adapter 1711 and the type, size, location, etc. of the reagent pump 1712 are determined based on the actual testing requirements by reference to the prior art, and will not be described herein again.

The adapter 1711 is sleeved with a sleeve 1711-1 for protecting the adapter 1711, the adapter 1711 and the sleeve 1711-1 are respectively arranged on the adapter mounting plate 1711-2 and the sleeve mounting plate 1711-3, an elastic element is arranged between the adapter mounting plate 1711-2 and the sleeve mounting plate 1711-3, and the sleeve mounting plate 1711-3 is connected with the pricking control component 1713, so that the sleeve 1711-1 can slide up and down relative to the adapter 1711, and the working state of the adapter 1711 can be adjusted.

When the adapter 1711 is connected with the tip01, the sleeve 1711-1 moves upwards under the action of the end face of the tip01, so that the sleeve mounting plate 1711-3 moves upwards, and the pricking control assembly 1713 is driven to move upwards; on the contrary, when the tip01 is withdrawn, the pricking control component 1713 is controlled to drive the sleeve mounting plate 1711-3 to descend, so that the sleeve 1711-1 descends along the adapter 1711, the tip01 is gradually separated from the adapter 1711, and the tip01 is completely separated from the adapter 1711 until the lower end face of the sleeve 1711-1 is flush with the lower end face of the adapter 1711.

The pricking control assembly 1713 comprises a linear displacement mechanism and a driving mechanism, the linear displacement mechanism specifically comprises a guide rail slider mechanism, a guide pillar and guide sleeve structure and the like, and the driving mechanism specifically comprises an electric driving mechanism such as a screw rod motor, a stepping motor, a hydraulic cylinder and an electric push rod.

Preferably, referring to fig. 19, the pricking control assembly 1713 includes a guide post 1713-1 having one end connected to the sleeve mounting plate 1711-3, a guide post connecting plate 1713-2 connected to the other end of the guide post 1713-1, and a screw motor 1713-3, wherein the guide post connecting plate 1713-2 is disposed below a motor shaft of the screw motor 1713-3. The type, number, size and position of the guide posts 1713-1, the type, model and position of the lead screw motor 1713-3, and the like are determined according to actual requirements by referring to the prior art, and are not described herein again.

The reagent needle translation assembly 1714 and the reagent needle lifting assembly comprise linear displacement mechanisms such as a guide rail sliding block mechanism, a synchronous belt mechanism and the like, and driving mechanisms such as a stepping motor and the like. Referring to fig. 18, the reagent needle translation unit 1714 includes a timing belt mechanism and a driving motor, and the driving motor drives the timing belt and the reagent needle connected to the timing belt to move in the Y-axis direction when rotating.

When the reagent needle assembly 171 transfers the extracted reagent, the reagent needle translation assembly 1714 drives the reagent needle to move along the Y-axis direction, so that the reagent needle directly faces the tip01 to be pricked; then, the reagent needle lifting assembly drives the reagent needle to descend along the Y axis, so that the lower end face of the adapter 1711 is contacted with the tip01, the sleeve 1711-1 moves upwards under the action of the tip01 to expose the adapter 1711, the adapter 1711 is matched and connected with the tip01, and the tip01 is pricked; after the pricking is finished, the reagent needle lifting assembly drives the reagent needle to move upwards along the Z axis, the reagent needle translation assembly 1714 drives the reagent needle to move right above a reagent to be sucked, the reagent needle lifting assembly drives the reagent needle to move downwards along the Z axis, and the liquid-transferring control assembly controls the reagent pump 1712 to suck liquid through tip01 connected to the adapter 1711; after the liquid absorption is finished, the reagent needle lifting assembly drives the reagent needle to move upwards along the Z axis, the reagent needle translation assembly 1714 drives the reagent needle to move above the extraction reagent strip 03, the gantry assembly 173 drives the reagent needle to move above the reaction bin 031 along the X axis, the reagent needle lifting assembly drives the reagent needle to move downwards along the Z axis, and the liquid transfer control assembly controls the reagent pump 1712 to inject the extraction reagent into the reaction bin 031 through the adapter 1711; after the injection is completed, the reagent needle lifting assembly drives the reagent needle to move upwards along the Z axis, the gantry assembly 173 drives the reagent needle to move to a waste hole, the pricking control assembly 1713 drives the sleeve 1711-1 to move downwards along the length direction of the adapter 1711 so as to separate the tip01 from the adapter 1711, and the tip01 is discarded.

Similarly, the reagent needle assembly 171 can be driven to fill the PCR tube 021 with the amplification reagents and the purified product by a similar operation. In addition, the reagent needle assembly 171 may be configured to prick the sealing plug 022 and cover the PCR tube 021 with the sealing plug 022.

In this embodiment, the reagent needle lifting assembly and the reagent needle translation assembly 1714 cooperate with the gantry assembly 173 to adjust the spatial coordinates of the adaptor 1711, so that the reagent needle assembly 171 can be adapted to pipetting the extraction reagent, pipetting the amplification reagent, and sealing the PCR tube 021; the pricking control component 1713 realizes automatic pricking and abandonment of tip01, and improves pipetting efficiency.

Preferably, in order to facilitate the control system to acquire the working state of the reagent needle, the reagent needle assembly 171 further comprises a tip taking detection component 1715 and a tip withdrawal detection component 1716, and when the guide pillar connecting plate 1713-2 moves up to the highest point, the tip taking detection component 1715 outputs a grabbing success signal; when the lower end surface of the sleeve 1711-1 is lowered to be flush with the lower end surface of the adapter 1711, the retract detection module 1716 outputs a retract success signal. The structure, size, position, connection mode, etc. of the tip taking detection module 1715 and the tip removing detection module 1716 are determined according to actual detection requirements, and are not described herein again.

On the basis of the above embodiment, the sample injection needle assembly 172 includes a plurality of liquid-transfering pumps 1721 arranged in parallel, a liquid-transfering pump lifting assembly 1723 for driving the liquid-transfering pump 1721 to lift, and a plunger lifting assembly 1722 for driving a plunger of the liquid-transfering pump 1721 to slide relative to a pump body of the liquid-transfering pump 1721, wherein the liquid-transfering pump 1721 is installed on the liquid-transfering pump lifting assembly 1723, and the plunger lifting assembly 1722 is connected with a plunger of the liquid-transfering pump 1721.

Referring to fig. 20, the sample injection needle assembly 172 is connected to the gantry assembly 173 through the sample injection needle mounting base, the liquid-transferring pump 1721 is mounted on the liquid-transferring pump mounting plate, and the plunger lifting assembly 1722 is mounted on the liquid-transferring fixing plate; be equipped with plunger lifting unit 1722 between liquid-transfering pump mounting panel and the pump infusion fixed plate, be equipped with liquid-transfering pump lifting unit 1723 between pump infusion fixed plate and the application of sample needle mount pad.

The pipetting pump 1721 comprises a pump body and a plunger arranged in the pump body, wherein the lower end of the pump body is used for matching with tip 01; the plunger is connected with the pump body in a sliding mode, so that the air pressure in the pump body is changed by changing the volume of the pump body, and the liquid pumping and injecting processes are achieved. The number, type, model, etc. of the liquid-transfer pumps 1721 are determined according to actual needs by referring to the prior art, and are not described herein again.

The liquid transfer pump lifting assembly 1723 and the plunger lifting assembly 1722 comprise a linear displacement mechanism and a driving mechanism, wherein the linear displacement mechanism comprises a guide rail slider mechanism, a synchronous belt mechanism and the like, and the driving mechanism comprises a stepping motor, a lead screw motor, a hydraulic cylinder, an electric push rod and the like.

Preferably, in order to control the plunger synchronous motion of a plurality of the pipetting pumps 1721, the plunger lifting assembly 1722 is provided with a synchronous bar which is clamped with the plungers of the pipetting pumps 1721.

When the sample adding needle assembly 172 transfers liquid, firstly, the gantry assembly 173 drives the sample adding needle assembly 172 to move to the upper part of the disposable tip consumable box 12 along the X axis, and the sample adding needle lifting assembly 1723 drives the liquid transferring pump 1721 to descend along the Z axis, so that the tip01 is installed at the lower end of the liquid transferring pump 1721; after the liquid transfer pump 1721 is matched with the tip01, the sample adding needle lifting assembly 1723 drives the liquid transfer pump 1721 to ascend along the Z axis, the gantry assembly 173 drives the sample adding needle assembly 172 to move above a reagent to be absorbed along the X axis, the sample adding needle assembly 1723 drives the liquid transfer pump 1721 to descend along the Z axis, and the plunger lifting assembly 172 drives the plunger to ascend along the Z axis relative to the pump body to finish liquid pumping; after liquid pumping is finished, the sample adding needle lifting assembly 1723 drives the liquid transferring pump 1721 to ascend along the Z axis, the gantry assembly 173 drives the sample adding needle assembly 172 to move to a position to be added with liquid along the X axis, the sample adding needle lifting assembly 1723 drives the liquid transferring pump 1721 to descend along the Z axis, and the plunger lifting assembly 172 drives the plunger to descend along the Z axis relative to the pump body to finish liquid injection; after liquid injection is completed, the sample injection needle lifting assembly 1723 drives the liquid transfer pump 1721 to ascend along the Z axis, the gantry assembly 173 drives the sample injection needle assembly 172 to move to a waste hole site along the X axis, the sample injection needle lifting assembly 1723 drives the liquid transfer pump 1721 to descend along the Z axis, and the plunger lifting assembly 172 drives the plunger to descend along the Z axis relative to the pump body, so that the ejector pin at the bottom of the plunger is exposed and presses the tip01 downwards, so that the tip01 is separated from the liquid transfer pump 1721, and automatic waste of the tip01 is completed.

In this embodiment, the plurality of liquid-transfer pumps 1721 are arranged in parallel in the sample-adding needle assembly 172, and the synchronization bar can drive the plungers of the plurality of liquid-transfer pumps 1721 to move synchronously, so that multi-channel synchronous tip installation, liquid transfer and tip abandonment are realized, a large number of repetitive operations during testing of a plurality of same reagents arranged on the same sample are avoided, the running time is saved, and the detection speed is greatly increased; the sample injection needle assembly 172 has a compact structure, saves installation space, and is beneficial to the miniaturization of a DNA and RNA co-extraction and amplification system.

Preferably, the sample feeding needle assembly 172 further comprises a pumping and injecting liquid detecting assembly 1724 for detecting the working state of the liquid transferring pump 1721 and a Z-axis origin detecting assembly 1725 for detecting whether the liquid transferring pump lifting assembly 1723 is reset. The types, installation manners, positions and the like of the pumping and injecting liquid detection assembly 1724 and the Z-axis origin detection assembly 1725 are determined according to actual detection requirements, and are not described herein again.

On the basis of the above embodiment, the PCR transfer device 21 includes a hand grip assembly 211 for gripping or releasing the PCR tube 021, an uncovering assembly 212 for opening or closing the amplification lids 314 of the PCR amplification device 31, an X-axis assembly 213 for driving the hand grip assembly 211 and the uncovering assembly 212 to move in the X-axis direction, a Y-axis assembly 214 for driving the hand grip assembly 211 and the uncovering assembly 212 to move in the Y-axis direction, and a Z-axis assembly 215 for driving the hand grip assembly 211 and the uncovering assembly 212 to move in the Z-axis direction; the gripper assembly 211 and the cover opening assembly 212 are respectively slidably connected to a Z-axis assembly 215, the Z-axis assembly 215 is slidably connected to a Y-axis assembly 214, and the Y-axis assembly 214 is slidably connected to an X-axis assembly 213.

It should be noted that the grip assembly 211 and the cover opening assembly 212 are respectively slidably connected to the Z-axis assembly 215, i.e., there is no direct connection therebetween.

The X-axis assembly 213, the Y-axis assembly 214 and the Z-axis assembly 215 all comprise linear displacement mechanisms and driving mechanisms, the linear displacement mechanisms are one or a combination of several linear displacement mechanisms such as synchronous belt mechanisms and guide rail sliding block mechanisms, and the driving mechanisms comprise common driving mechanisms such as stepping motors, electric push rods and hydraulic cylinders. The specific structures, sizes, positions, connection modes and the like of the X-axis assembly 213, the Y-axis assembly 214 and the Z-axis assembly 215 are determined according to actual detection requirements, and are not described herein again.

When the PCR transfer device 21 works, the X shaft assembly 213 and the Y shaft assembly 214 drive the Z shaft assembly 215 to move to a position right above the amplification heating block 311 of the PCR amplification device 31; the Z shaft assembly 215 drives the cover opening assembly 212 to move downwards along the Z shaft, so that the cover opening assembly 212 is clamped and fixed with the amplification cover 314; the Y-axis assembly 214 drives the gripper assembly 211 and the uncovering assembly 212 to move along the Y-axis direction, so that the amplification cover 314 is separated from the amplification heating block 311 of the PCR amplification device 31, and the uncovering operation is completed;

the Z-axis assembly 215 drives the gripper assembly 211 to descend to a lower position (gripping position) and the uncapping assembly 212 to ascend to a higher position; the X-axis assembly 213 and the Y-axis assembly 214 drive the gripper assembly 211 to move to the position of the PCR consumable box 13, and the gripper assembly 211 grips the PCR tube 021; the X-axis assembly 213 and the Y-axis assembly 214 drive the gripper assembly 211 to move to the amplification heating block 311 of the PCR amplification device 31, and the gripper assembly 211 releases the PCR tube 021, so that the PCR tube 021 is placed in the amplification hole of the amplification heating block 311;

after the PCR tube 021 in the reaction chamber 031 is full, the Z-axis assembly 215 drives the lid-opening assembly 212 to descend, the gripper assembly 211 to ascend, and the lid-opening assembly 212 closes the amplification lid 314.

After the amplification reaction is completed, the PCR transfer device 21 takes out the reacted PCR tube 021 according to the similar operation described above, discards it to the disposal position, and finally closes the amplification lid 314.

In this embodiment, the PCR transfer device 21 realizes the three-dimensional movement of the PCR tube 021 and the automatic operation of opening and closing the amplification cover 314, and the gripper assembly 211 and the cover opening assembly 212 are controlled by the same motor and alternately reciprocate to move up and down, which is beneficial to reducing the volume of the device, improving the gripping efficiency, and effectively improving the operation efficiency and the use effect of the PCR transfer device 21.

On the basis of the above embodiment, the gripper assembly 211 comprises a gripper base plate, a gripper movable plate arranged below the gripper base plate in parallel, a gripper for gripping the PCR tube 021 and a retraction driving assembly for controlling the gripper to open or retract, the retraction driving assembly is arranged on the gripper base plate, and a telescopic end of the retraction driving assembly passes through the gripper base plate to be connected with the gripper movable plate so as to drive the gripper movable plate to ascend or descend; the hand grip base plate and the hand grip movable plate are both connected with the contraction driving assembly, when the hand grip movable plate descends, the hand grip is opened, and when the hand grip movable plate ascends, the hand grip contracts.

The shape, the structure, the size, the material, the position and the like of the hand grip bottom plate, the hand grip movable plate, the hand grip and the retraction driving assembly are determined according to actual conditions and actual requirements.

When the retraction driving assembly moves in a retraction mode, the retraction end of the retraction driving assembly drives the hand grip movable plate to descend to drive the hand grip to open, so that the hand grip can conveniently grip the PCR tube 021; when the telescopic end of the retraction driving assembly drives the gripper movable plate to ascend, the gripper movable plate drives the gripper to retract and close so that the gripper can grasp the PCR tube 021 tightly. After the hand grip grasps the PCR tube 021, the X-axis assembly 213, the Y-axis assembly 214 and the Z-axis assembly 215 move the PCR tube 021 to the desired position.

In this embodiment, the PCR tube transferring device 21 effectively improves the grabbing and transferring efficiency of the PCR tube 021, ensures the accuracy of the grabbing and transferring operation of the PCR tube 021, and has the advantages of simple structure, convenient operation and easy popularization.

To avoid contamination of the nucleic acid sample in the PCR tube 021 during movement, the PCR tube 021 is sealed with a sealing plug 022, and the sealing plug 022 is opened after the PCR tube 021 is transferred into place.

Preferably, the gripper assembly 211 further comprises a guide post and a top sleeve, wherein the guide post is used for being clamped with a sealing plug 022 at the top of the PCR tube 021, and the top sleeve is used for pushing back the sealing plug 022; the guide column penetrates through the gripper movable plate and is fixedly connected with the gripper base plate, and the guide column is positioned on the axis of the gripper; the top sleeve is sleeved on the periphery of the guide post and is fixedly connected with the gripper movable plate.

When the contraction driving assembly drives the gripper movable plate to descend, the gripper is opened, the top sleeve fixed on the gripper movable plate descends along with the gripper movable plate, and the top sleeve pushes and retreats the sealing plug 022 at the top of the PCR tube 021, so that the PCR tube 021 is pushed and retreated; when the shrinkage driving assembly drives the gripper movable plate to ascend, the gripper shrinks and closes, the guide column is fixed, the top sleeve moves upwards along with the gripper movable plate, the guide column at the moment is exposed and inserted into the sealing plug 022, the guide column and the sealing plug 022 are clamped and fixed, and the gripping of the PCR tube 021 is realized. After the hand grip grasps the PCR tube 021, the X-axis assembly 213, the Y-axis assembly 214 and the Z-axis assembly 215 move the PCR tube 021 to the desired position.

The hand grip and the ejection sleeve are driven to move by the retraction driving assembly, so that the hand grip and the guide column are matched to grip the PCR tube 021 and effectively eject the PCR tube 021 by the ejection sleeve, the operation efficiency of the device is effectively improved, and pollution caused by manual intervention is prevented. In addition, the device is beneficial to reducing the volume and occupying less space.

Preferably, the grip assembly 211 further includes a retreat detecting assembly for detecting whether the sealing plug 022 is successfully retreated. The structure, size, position, etc. of the pushing detection assembly are determined according to actual needs, and are not described herein again.

On the basis of the above embodiment, the PCR amplification device 31 includes an amplification heating block 311, an amplification heating sheet 312, an amplification heat sink 313, an amplification cover 314 for covering the amplification heating block 311, and an amplification cooling component 315 for improving the heat dissipation efficiency of the amplification heat sink 313, the amplification heating block 311 is provided with at least two amplification holes for placing the PCR tube 021, and the amplification cover 314, the amplification heating block 311, the amplification heating sheet 312, and the amplification heat sink 313 are sequentially arranged from top to bottom.

Referring to fig. 25, the number of the amplification heating blocks 311 may be one, two or more, and each amplification heating block 311 may have a plurality of amplification holes therein; each amplification heating block 311 is heated corresponding to one amplification heating sheet 312, so that the device cost is reduced; an amplification cooling component 315 is arranged below the amplification heat radiator 313 so as to improve the heat dissipation efficiency of the amplification heat radiator 313, thereby improving the rise and fall speed of the PCR amplification temperature and reducing the amplification time.

Preferably, the amplification heating block 311 is provided with an amplification temperature sensor for detecting the temperature of the amplification heating block 311 in real time, so that a detection person can grasp the temperature of the amplification heating block 311 in real time, thereby effectively controlling the heating temperature.

The shape, structure, size, material, position, etc. of the amplification heating block 311, amplification heating sheet 312, amplification heat sink 313, amplification cover 314, amplification cooling member 315, and amplification temperature sensor are determined according to actual needs with reference to the prior art.

When the PCR amplification device 31 works, firstly, the amplification cover 314 is opened, and the PCR tube 021 containing the nucleic acid sample is placed in the amplification hole of the amplification heating block 311; after the PCR tube 021 is placed, the amplification cover 314 is covered, and the amplification heating block 311 is heated by the amplification heating sheet 312, so that the PCR amplification operation is performed on the nucleic acid sample.

When multiple detections are performed on the same nucleic acid sample, a single nucleic acid needs to be divided into multiple parts for amplification, but the PCR amplification device 31 provided in this embodiment has multiple amplification holes in a single amplification heating block 311, and the heating temperature in each amplification heating block 311 is kept consistent, which is beneficial to ensuring that the amplification conditions of the same nucleic acid sample are the same, and reducing the amplification result deviation of the sample caused by different amplification temperatures; after the amplification heating block 311 is covered by the amplification cover 314, the amplification heating block 311 and the sample can be insulated in the amplification process, so that the temperature rise speed is higher, and the amplification efficiency is higher; in addition, the amplification cooling component 315 effectively improves the heat dissipation efficiency of the amplification heat sink 313, further improves the amplification temperature rise and fall speed of the sample, reduces the amplification time of the sample, and improves the amplification efficiency of the sample.

Preferably, the amplification cover 314 comprises a frame and a top cover capable of sliding transversely along the frame, the top cover and the amplification heating blocks 311 are arranged in a one-to-one correspondence manner, the frame is provided with a cover opening sensor for detecting whether the top cover is opened, and the top cover is provided with a cover opening blocking piece for triggering the cover opening sensor. The structures, materials, sizes, positions and the like of the frame, the top cover, the cover opening sensor and the cover opening baffle are determined according to actual needs, and are not described in detail herein.

Under the condition of closing the cover, the positions of the cover opening blocking piece correspond to those of the cover opening sensor, and the cover opening blocking piece triggers the cover opening sensor; under the state of uncapping, the separation blade of uncapping slides along with the top cap, uncapping separation blade and uncapping sensor separation, and the separation blade of uncapping can not trigger the sensor of uncapping.

The real-time fluorescence quantitative detection PCR technology is a method for adding a fluorescent group into a PCR reaction system, monitoring the whole PCR process in real time by utilizing fluorescent signal accumulation, and finally carrying out quantitative analysis on an unknown template through a standard curve. The technology detects the PCR product by means of the fluorescent signal, on one hand, the sensitivity of the polymerase chain reaction is improved, on the other hand, the polymerase chain reaction collects one datum every cycle, so that a real-time nucleic acid sample amplification curve can be established, and the CT value can be accurately determined, so that the initial nucleic acid copy number can be determined according to the CT value, and the quantitative detection of the nucleic acid can be really realized.

In addition to the above embodiments, the fluorescence detection device 32 includes a light source assembly 321 for providing a fluorescence light source, a fluorescence detection assembly 322 for performing quantitative detection, a light source fiber 323, and a fluorescence detection fiber 324, wherein one side of the amplification hole is connected to one end of the light source fiber 323, and the other side of the amplification hole is connected to one end of the fluorescence detection fiber 324; the other end of the light source fiber 323 is connected to the light source assembly 321, and the other end of the fluorescence detection fiber 324 is connected to the fluorescence detection assembly 322.

The structures, materials, dimensions, positions, connection modes and the like of the light source assembly 321, the fluorescence detection assembly 322, the light source fiber 323 and the fluorescence detection fiber 324 are determined according to actual detection requirements.

Preferably, the light source fiber 323 and the fluorescence detection fiber 324 are respectively connected to two sides of the amplification heating block 311, and the included angle between the light source fiber 323 and the fluorescence detection fiber 324 is 90 °, so that each fiber is accurately and firmly positioned, which is beneficial to the accuracy of the light measurement value.

It should be noted that the first and second magnets and the first and second magnetic attraction surfaces 1621-1 and 1621-2 mentioned in this document are only used to distinguish the difference of the positions and do not contain any limitation to the sequence.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The DNA and RNA nucleic acid co-extraction and detection system provided by the utility model is described in detail above. The principles and embodiments of the present invention have been explained herein using specific examples, and the above descriptions of the embodiments are only used to help understand the method and its core ideas of the present invention. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, the present invention can be further modified and modified, and such modifications and modifications also fall within the protection scope of the appended claims.

Claims (13)

1. A DNA and RNA nucleic acid co-extraction and detection system is characterized by comprising a nucleic acid extraction system, a sealing, transferring and mixing system, a nucleic acid detection system and a control system, wherein the nucleic acid extraction system, the sealing, transferring and mixing system and the nucleic acid detection system are all connected with the control system;

the nucleic acid extraction system comprises a basket (11) for loading an extraction reagent strip (03) and a sample tube (04), a consumable supply module, a kit loading module (14), a bar code scanning device (15) for automatically inputting bar code information, a magnetic incubation device (16) and an automatic sample adding device (17) for adding liquid into a reaction bin (031) or a PCR tube (021) of the extraction reagent strip (03) and sealing the PCR tube (021);

the sealing and transferring system comprises a PCR transfer device (21) and a high-speed blending device (22), wherein the PCR transfer device is used for grabbing and driving the PCR tube (021) to move;

the nucleic acid detection system comprises a PCR amplification device (31) and a fluorescence detection device (32).

2. The DNA, RNA nucleic acid co-extraction and detection system according to claim 1, wherein the basket (11) comprises a basket cover plate (112), a basket bottom plate (111) for containing the extraction reagent strip (03), and a sample holder (113) for containing the sample tube (04), wherein the sample holder (113) is detachably connected with the basket bottom plate (111);

the basket cover plate (112) is rotatably connected with the basket bottom plate (111) so as to limit the displacement of the extracted reagent strip (03) in the height direction when the basket cover plate (112) is jointed with the basket bottom plate (111).

3. The DNA and RNA nucleic acid co-extraction and detection system according to claim 1, wherein the barcode scanning device (15) comprises a mounting bracket (151), a scanning assembly (153), a pushing assembly (154) for pushing the extraction reagent strip (03) between a code scanning position and a standby position, a pushing movement assembly (152) for driving the pushing assembly (154) to move along the length direction of the mounting bracket (151), and a detection assembly (155), wherein the detection assembly (155) is used for judging whether the extraction reagent strip (03) and the sample tube (04) exist in the current channel, the detection assembly (155) is in signal connection with the pushing movement assembly (152), and the scanning assembly (153) and the detection assembly (155) are both mounted on a mounting base plate of the pushing assembly (154).

4. The co-extraction and detection system for DNA and RNA nucleic acid according to claim 1, wherein the magnetic attraction incubation device (16) comprises an incubation module (161) for heating the reaction chamber (031), a magnetic attraction module (162) for magnetically attracting the reaction chamber (031), and a driving component (163), wherein the incubation module (161) and the magnetic attraction module (162) are both connected with the driving component (163), and the driving component (163) is used for driving the incubation module (161) and the magnetic attraction module (162) to alternately approach the reaction chamber (031).

5. The DNA and RNA nucleic acid co-extraction and detection system according to claim 4, wherein the incubation module (161) comprises a heating block (1611) having a reagent tank (1611-1), a heating assembly (1612) attached to an inner wall surface of the reagent tank (1611-1), a heat dissipation assembly (1613) disposed at a bottom of the heating block (1611), and a temperature sensor (1614) for detecting a temperature of the heating block (1611), wherein the temperature sensor (1614) is connected to the heating block (1611).

6. The DNA, RNA nucleic acid co-extraction and detection system according to claim 4, wherein the magnetic module (162) comprises a magnet (1621) and a rotating component (1622) for driving the magnet (1621) to rotate, wherein the rotating component (1622) is connected with the magnet (1621);

magnet (1621) include that first magnetism inhales face (1621-1) and second magnetism and inhale face (1621-2), first magnetism inhale face (1621-1) with the two area size difference of face (1621-2) are inhaled to the second magnetism and all are used for adsorbing the magnetic bead in reaction bin (031).

7. The DNA, RNA nucleic acid co-extraction and detection system according to claim 1, wherein the PCR transfer apparatus (21) comprises a gripper assembly (211) for gripping or releasing a PCR tube (021), a cap-opening assembly (212) for opening or closing an amplification cap (314) of the PCR amplification apparatus (31), an X-axis assembly (213) for driving the gripper assembly (211) and the cap-opening assembly (212) to move in an X-axis direction, a Y-axis assembly (214) for driving the gripper assembly (211) and the cap-opening assembly (212) to move in a Y-axis direction, and a Z-axis assembly (215) for driving the gripper assembly (211) and the cap-opening assembly (212) to move in a Z-axis direction;

the hand grip assembly (211) and the cover opening assembly (212) are respectively connected to the Z shaft assembly (215) in a sliding mode, the Z shaft assembly (215) is connected to the Y shaft assembly (214) in a sliding mode, and the Y shaft assembly (214) is connected to the X shaft assembly (213) in a sliding mode.

8. The DNA, RNA nucleic acid co-extraction and detection system according to claim 7, wherein the gripper assembly (211) comprises a gripper base plate, a gripper movable plate arranged in parallel below the gripper base plate, a gripper for gripping a PCR tube (021) and a retraction driving assembly for controlling the gripper to open or retract, the retraction driving assembly is arranged on the gripper base plate, and a telescopic end of the retraction driving assembly penetrates through the gripper base plate to be connected with the gripper movable plate so as to drive the gripper movable plate to ascend or descend;

the hand grip base plate and the hand grip movable plate are both connected with the retraction driving assembly, the hand grip is opened when the hand grip movable plate descends, and the hand grip is retracted when the hand grip movable plate ascends.

9. The DNA and RNA nucleic acid co-extraction and detection system according to claim 1, wherein the PCR amplification device (31) comprises an amplification heating block (311), an amplification heating sheet (312), an amplification radiator (313), an amplification cover (314) for covering the amplification heating block (311), and an amplification cooling component (315) for improving the heat dissipation efficiency of the amplification radiator (313), the amplification heating block (311) is provided with at least two amplification holes for placing PCR tubes (021), and the amplification cover (314), the amplification heating block (311), the amplification heating sheet (312), and the amplification radiator (313) are sequentially arranged from top to bottom.

10. The DNA, RNA nucleic acid co-extraction and detection system according to claim 9, wherein the fluorescence detection device (32) comprises a light source assembly (321) for providing a fluorescence light source, a fluorescence detection assembly (322) for quantitative detection, a light source fiber (323), and a fluorescence detection fiber (324), one side of the amplification well is connected to one end of the light source fiber (323), and the other side of the amplification well is connected to one end of the fluorescence detection fiber (324);

the other end of the light source optical fiber (323) is connected with the light source assembly (321), and the other end of the fluorescence detection optical fiber (324) is connected with the fluorescence detection assembly (322).

11. The DNA, RNA nucleic acid co-extraction and detection system according to any one of claims 1 to 10, wherein the automated sample application device (17) comprises a reagent needle assembly (171) for reagent extraction and injection, a sample needle assembly (172) for sample extraction and injection, and a gantry assembly (173), wherein the reagent needle assembly (171), the sample needle assembly (172), and the gantry assembly (173) are connected to the control system;

the reagent needle assembly (171) and the sample injection needle assembly (172) are respectively arranged at two sides of the gantry assembly (173), and the gantry assembly (173) can move along the lifting basket (11), the consumable supply module and the reagent box loading module (14).

12. The DNA, RNA nucleic acid co-extraction and detection system according to claim 11, wherein the reagent needle assembly (171) comprises a reagent needle, a pricking control component (1713), a pipetting control component, a reagent needle lifting component, and a reagent needle translation component (1714), the reagent needle comprises an adapter (1711) and a reagent pump (1712) in communication with the adapter (1711), the reagent pump (1712) is connected with the pipetting control component, the adapter (1711) is connected with the pricking control component (1713), so that the pricking control component (1713) controls the adapter (1711) to prick or withdraw tip (01) or sealing plug (022);

the reagent needle translation assembly (1714) is used for driving the reagent needle to move along the horizontal plane and the vertical direction of the movement direction of the gantry assembly (173), and the reagent needle lifting assembly is used for driving the reagent needle to move along the vertical direction.

13. The system for co-extracting and detecting DNA and RNA nucleic acid according to claim 11, wherein the sample injection needle assembly (172) comprises a plurality of liquid transfer pumps (1721) arranged in parallel, a liquid transfer pump lifting assembly (1723) for driving the liquid transfer pumps (1721) to lift, and a plunger lifting assembly (1722) for driving a plunger of the liquid transfer pumps (1721) to slide relative to a pump body of the liquid transfer pumps (1721), the liquid transfer pumps (1721) are mounted on the liquid transfer pump lifting assembly (1723), and the plunger lifting assembly (1722) is connected with a plunger of the liquid transfer pumps (1721).

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