CN212410161U

CN212410161U - Whole blood sample collection container for mycobacterium tuberculosis gamma interference in-vitro release test

Info

Publication number: CN212410161U
Application number: CN202021187923.XU
Authority: CN
Inventors: 张文星; 杨高举; 韩晶
Original assignee: Autobio Diagnostics Co Ltd
Current assignee: Autobio Diagnostics Co Ltd
Priority date: 2020-06-24
Filing date: 2020-06-24
Publication date: 2021-01-26
Anticipated expiration: 2030-06-24

Abstract

The utility model discloses a whole blood sample collecting container for a mycobacterium tuberculosis gamma interference matrix external release test, which comprises a supporting seat, wherein the upper surface of the supporting seat is provided with a plurality of supporting grooves, and a test subject background test tube, a test subject immunity state test tube and a test subject detection tube are respectively clamped in the supporting grooves; the test tube for the background of the testee, the test tube for the immune state of the testee and the test tube for the detection of the testee have the same structure and comprise transparent tube bodies with upper openings, the upper openings of the tube bodies are hermetically buckled with rubber plugs, and the upper ends of the tube bodies outside the rubber plugs are buckled with protective covers with distinguishing coatings on the outer surfaces. The utility model has the advantages of simple structure and simple and convenient processing and manufacturing. The operation flow is simplified, the workload is reduced, other uncertain factors can be effectively avoided being introduced in the whole blood subpackaging process, the anti-interference capability is improved, pollution is avoided, and the detection result is ensured to be normal and accurate; the method is convenient for quick identification and reduces operation errors; the harvesting of the supernatant is convenient, and the device is matched with a full-automatic instrument.

Description

Whole blood sample collection container for mycobacterium tuberculosis gamma interference in-vitro release test

Technical Field

The utility model belongs to the technical field of the external detection technique and specifically relates to a mycobacterium tuberculosis gamma disturbs matrix and releases whole blood sample collecting container for experiment outward.

Background

Tuberculosis is a global serious public health problem, the diagnosis of tuberculosis is mainly based on clinical symptoms, chest X-ray pictures and bacteriological examination at present, the traditional diagnosis methods are time-consuming and have high omission ratio, and the optimal time for early discovery and early treatment is delayed. With the continuous understanding of the role of interferon gamma in regulating the immune response of tuberculosis-infected cells, it is gradually recognized that interferon gamma release assays (IGGRAs) can be used for the auxiliary diagnosis of tuberculosis infection, and especially plays a very important role in the diagnosis of latent tuberculosis.

Gamma interferon in vitro release test for the diagnosis of tuberculosis infection is divided into two main steps: a stimulation phase and a detection phase; at present, each detection mechanism adopts the steps of firstly drawing blood and then subpackaging, the whole blood subpackaging has strict requirements on the environment, a secondary biological safety cabinet is generally adopted to protect a sample and an operator, the operation mode not only increases the cost of departments, but also easily causes certain damage to the operator, and meanwhile, the whole blood subpackaging process is easy to pollute and causes abnormal detection results.

Disclosure of Invention

The utility model aims to provide a simple structure, convenient to use's mycobacterium tuberculosis gamma disturbs matrix and releases experiment with whole blood sample collection container in vitro.

In order to achieve the above purpose, the utility model can adopt the following technical proposal:

the utility model discloses a mycobacterium tuberculosis gamma interferes matrix external release test with whole blood sample collection container, including the upper surface set up a plurality of support seats bearing the support seat of groove in the bearing inslot card respectively has the testee background test tube of holding in the separable glue, anticoagulant, 5-200 UL buffering matrix culture solution, and the testee immune state test tube of holding in the separable glue, anticoagulant, 5-200 UL mitogen class protein buffering matrix culture solution and the testee detecting tube of holding in the separable glue, anticoagulant, containing stimulating antigen class protein buffering matrix culture solution; the test tube for the background of the testee, the test tube for the immune state of the testee and the test tube for the detection of the testee have the same structure and comprise transparent tube bodies with upper openings, the upper openings of the tube bodies are hermetically buckled with rubber plugs, and the upper ends of the tube bodies outside the rubber plugs are buckled with protective covers with distinguishing coatings on the outer surfaces.

The three bearing grooves are in a group, and a plurality of groups are uniformly arranged at intervals along the transverse and longitudinal directions of the upper surface of the supporting seat.

The tube body is a round bottom glass test tube or a round bottom plastic test tube.

The rubber plug is a rubber sealing plug or a silica gel sealing plug buckled at the upper opening of the tube body.

The tube wall of the test tube for the background of the test subject is pasted with a green label, and the distinguishing coating on the outer surface of the protective cover at the upper end of the test tube is a green coating; the tube wall of the test tube for the immune state of the subject is pasted with a red label, and the distinguishing coating on the outer surface of the protective cover at the upper end of the test tube is a red coating; the tube wall of the test tube of the testee is pasted with a yellow label, and the distinguishing coating on the outer surface of the protective cover at the upper end of the test tube is a yellow coating.

The utility model has the advantages of simple structure and simple and convenient processing and manufacturing. The liquid outlet end of the blood taking needle is directly inserted into the rubber plug for blood taking, so that the operation flow of a gamma interference in-vitro release test is simplified, the workload is reduced, other uncertain factors can be effectively avoided being introduced in the whole blood subpackaging process, the anti-interference capability is improved, the pollution is avoided, and the normal and accurate detection result is ensured; in addition, the protective cover with the zone coating is convenient for rapidly identifying the background test tube of the testee, the immune state test tube of the testee and the test tube of the testee, thereby reducing the operation error; meanwhile, the cell layer and the supernatant can be separated by the separation gel in the test tube for the background of the testee, the test tube for the immune state of the testee and the test tube for the test of the testee, so that the supernatant can be conveniently harvested and matched with a full-automatic instrument.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic view of another embodiment of the support base of fig. 1.

Detailed Description

As shown in fig. 1, the whole blood sample collection container for the mycobacterium tuberculosis gamma interference in vitro release test comprises a support seat 2 with three support grooves 1 arranged on the upper surface side by side, wherein a test subject background test tube, a test subject immune state test tube and a test subject detection tube are respectively clamped in the three support grooves 1; certainly, for the convenience of bearing multiunit test tube simultaneously, as shown in fig. 2, bearing groove 1 is three for a set of, lays a plurality of groups along the even interval arrangement of the upper surface horizontal, the vertical direction of supporting seat 2, improves the bearing capacity of supporting seat 2 greatly.

The test tube for the background of the subject is filled with separation gel, anticoagulant and 5-200 UL buffer matrix culture solution, the test tube for the immune state of the subject is filled with the separation gel, the anticoagulant and 5-200 UL buffer matrix culture solution containing mitogen protein, and the test tube for the subject is filled with the separation gel, the anticoagulant and the buffer matrix culture solution containing stimulating antigen protein; the mass of the separation gel filled in the test tube for the background of the testee, the test tube for the immune state of the testee and the test tube for the detection of the testee is 0.5-1.0 g, and the separation gel is directly added to the bottom of the original tube.

In addition, the test tube for the background of the testee, the test tube for the immune state of the testee and the test tube for the detection of the testee have the same structure and comprise transparent tube bodies 3 with upper openings, and the tube bodies 3 are round-bottom glass test tubes or round-bottom plastic test tubes. Sealed lock at the upper shed department of body 3 has plug 4, and plug 4 is the lock in the rubber sealing plug or the silica gel sealing plug of 3 upper shed departments of body, makes things convenient for the drainage needle directly to pierce through plug 4, carries out the partial shipment of blood and gathers under sealed environment, effectively avoids introducing other uncertain factors at whole blood partial shipment in-process, promotes the interference killing feature, avoids polluting, ensures that the testing result is normal, accurate. The upper end of the tube body 3 outside the rubber plug 4 is buckled with a protective cover 5 with a distinguishing coating on the outer surface, of course, according to the equipment condition of the unit, the distinguishing coating on the outer surface of the protective cover 5 on the background test tube of the testee is a green coating, the distinguishing coating on the outer surface of the protective cover 5 on the immune state test tube of the testee is a red coating, and the distinguishing coating on the outer surface of the protective cover 5 on the upper end of the test tube of the testee is a yellow coating; in addition, a green label 6 can be further pasted on the tube wall of the test tube for the background of the testee, a red label 7 is pasted on the tube wall of the test tube for the immune state of the testee, and a yellow label 8 is pasted on the tube wall of the test tube for the immune state of the testee, so that the test tube for the background of the testee, the test tube for the immune state of the testee and the test tube for the testee can be conveniently and rapidly identified, and the operation error is reduced.

Claims

1. A kind of tubercle bacillus gamma interferes with the body and releases the experimental whole blood sample collecting vessel in vitro, characterized by that: the device comprises a supporting seat (2) with a plurality of supporting grooves (1) on the upper surface, a test subject background test tube filled with separation gel, anticoagulant and 5-200 UL buffer matrix culture solution is respectively clamped in each supporting groove (1), a test subject immune state test tube filled with separation gel, anticoagulant and 5-200 UL mitogen-containing protein buffer matrix culture solution, and a test subject detection tube filled with separation gel, anticoagulant and stimulating antigen-containing protein buffer matrix culture solution; the test tube for the background of the testee, the test tube for the immune state of the testee and the test tube for the detection of the testee have the same structure and respectively comprise a transparent tube body (3) with an upper opening, a rubber plug (4) is hermetically buckled at the upper opening of the tube body (3), and a protective cover (5) with a distinguishing coating on the outer surface is buckled at the upper end of the tube body (3) outside the rubber plug (4).

2. The mycobacterium tuberculosis gamma interference whole blood sample collection container for in vitro release test of claim 1, wherein: the three supporting grooves (1) are in a group, and a plurality of groups are uniformly arranged at intervals along the transverse and longitudinal directions of the upper surface of the supporting seat (2).

3. The mycobacterium tuberculosis gamma interference whole blood sample collection container for in vitro release test of claim 1 or 2, wherein: the tube body (3) is a round bottom glass test tube or a round bottom plastic test tube.

4. The mycobacterium tuberculosis gamma interference whole blood sample collection container for in vitro release test of claim 1 or 2, wherein: the rubber plug (4) is a rubber sealing plug or a silica gel sealing plug buckled at the upper opening of the tube body (3).

5. The mycobacterium tuberculosis gamma interference whole blood sample collection container for in vitro release test of claim 1 or 2, wherein: the tube wall of the test tube for the background of the test subject is pasted with a green label (6), and the distinguishing coating on the outer surface of the protective cover (5) at the upper end of the test tube is a green coating; the tube wall of the test tube for the immune state of the testee is pasted with a red label (7), and the distinguishing coating on the outer surface of the protective cover (5) at the upper end of the test tube is a red coating; the tube wall of the test tube of the testee is pasted with a yellow label (8), and the distinguishing coating on the outer surface of the protective cover (5) at the upper end of the test tube is a yellow coating.