CN203083997U - ABO and RhD blood group antigen detection card - Google Patents
ABO and RhD blood group antigen detection card Download PDFInfo
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- CN203083997U CN203083997U CN 201220687920 CN201220687920U CN203083997U CN 203083997 U CN203083997 U CN 203083997U CN 201220687920 CN201220687920 CN 201220687920 CN 201220687920 U CN201220687920 U CN 201220687920U CN 203083997 U CN203083997 U CN 203083997U
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Abstract
The utility model relates to an ABO and RhD blood group antigen detection card which comprises a fixing plate (1), wherein eight micro-column type gel tubes (2) are arranged on the fixing plate (1) from left to right; two baffles (3) are arranged on the outer wall of each micro-column type gel tube (2); a protective film (4) is arranged above the fixing plate (1); an anti-A mouse monoclonal antibody (6), an anti-B mouse monoclonal antibody (7) and an anti-D mouse monoclonal antibody (8) are respectively arranged in reaction cavity (2.2) of the first to the third micro-column type gel tubes (2) and the fifth to the seventh micro-column type gel tubes (2); and the reaction cavities (2.2) of the fourth and eighth micro-column type gel tubes (2) are hollow cavities. According to the ABO and RhD blood group antigen detection card, the eight micro-column type gel tubes are divided into two tests, a control tube is added, the test flux is increased, the working efficiency is improved, and the testing result is well guaranteed.
Description
Technical field
The utility model relates to a kind of ABO, RhD blood group antigens test card.
Background technology
Safe, effective in order to ensure blood used in clinic, " the clinical blood transfusion technical manual " of the promulgation in 2000 of China Ministry of Public Health requires before the blood transfusion receptor and blood donor's abo blood group to be checked, and routine inspection patient RhD blood group.At present the detection method of abo blood group and RhD blood group antigens has slide method, test tube method, micro-column gel block-regulations etc. usually, and slide method, test tube method have sensitivity low, be difficult for standardization and batch detection, test findings and be subjected to shortcomings such as subjective influence factor is more; ABO, RhD blood group antigens test card adopt the micro-column gel immunoassay technology, are used for the detection of people ABO, RhD blood group antigens, and it is higher to have assay sensitivity, operational standardization, good reproducibility is grasped easily, can reduce experimental error, advantage such as the result is more accurate, stable.And ABO in the market, RhD blood group antigens test card are generally 6 hole cards, and do not have the negative control pipe, thereby influence test findings.
Summary of the invention
The purpose of this utility model is to overcome above-mentioned deficiency, and a kind of micro-column gel card of 8 holes is provided, and has not only increased control tube, warranty test result's accuracy, validity in the process that detects; Meet the requirement of " medical institutions' clinical laboratory management method " the 25 of the Ministry of Public Health in 2006 issuing and implementation simultaneously.
The purpose of this utility model is achieved in that
A kind of ABO, RhD blood group antigens test card, comprise fixed head, be provided with eight microtrabeculae type gel tubes on the described fixed head from left to right, each microtrabeculae type gel tube includes convex body, reaction chamber and gel separation post from top to bottom, described convex body is positioned at the top of fixed head upper edge, and reaction chamber and gel separation post all are positioned at the below of fixed head upper edge; All fill sephadex in the gel separation post of described eight microtrabeculae type gel tubes, be provided with anti-A mouse monoclonal antibody, anti-B mouse monoclonal antibody and anti-D mouse monoclonal antibody in the reaction chamber of the first to the 3rd, the 5th to the 7th microtrabeculae type gel tube respectively; In the reaction chamber of the 4th and the 8th microtrabeculae type gel tube is cavity.
The utility model ABO, RhD blood group antigens test card, fixed head is the acrylic plastering transparent panel.
The utility model ABO, RhD blood group antigens test card, the outer wall of described each microtrabeculae type gel tube is provided with two baffle plates, lays respectively at the front and back of fixed head.
The utility model ABO, RhD blood group antigens test card, the top of described fixed head is provided with diaphragm, and diaphragm covers on the convex body of eight microtrabeculae type gel tubes.
Compared with prior art, the beneficial effects of the utility model are:
The utility model ABO, eight microtrabeculae types of RhD blood group antigens test card gel tube are divided into 2 tests, have added control tube, have increased the test flux, have improved work efficiency, good assurance test findings; Diaphragm can well be protected the mouse monoclonal antibody in the microtrabeculae type gel tube in addition, thereby the infection of avoiding being subjected to foreign matters such as dust, bacterium in transportation causes inactivation; Being provided with of baffle plate makes things convenient for fixed head to be stuck on the support, is convenient to operation.
Description of drawings
Fig. 1 is the structural representation of the utility model ABO, RhD blood group antigens test card.
Wherein:
Fixed head 1
Microtrabeculae type gel tube 2
Convex body 2.1
Reaction chamber 2.2
Gel separation post 2.3
Baffle plate 3
Sephadex 5
Anti-A mouse monoclonal antibody 6
Anti-B mouse monoclonal antibody 7
Anti-C mouse monoclonal antibody 8.
Embodiment
Referring to Fig. 1, the utility model ABO, RhD blood group antigens test card, comprise fixed head 1, fixed head 1 is the acrylic plastering transparent panel, be provided with eight microtrabeculae type gel tubes 2 on the described fixed head 1 from left to right, described each microtrabeculae type gel tube 2 includes convex body 2.1, reaction chamber 2.2 and gel separation post 2.3 from top to bottom, and described convex body 2.1 is positioned at the top of fixed head 1 upper edge, and reaction chamber 2.2 and gel separation post 2.3 all are positioned at the below of fixed head 1 upper edge; The outer wall of described each microtrabeculae type gel tube 2 is provided with two baffle plates 3, lays respectively at the front and back of fixed head 1; The top of described fixed head 1 is provided with diaphragm 4, and diaphragm 4 covers on the convex body 2.1 of eight microtrabeculae type gel tubes 2.
All fill sephadex 5 in the gel separation post 2.3 of described eight microtrabeculae type gel tubes 2.
Be provided with anti-A mouse monoclonal antibody 6, anti-B mouse monoclonal antibody 7 and anti-D mouse monoclonal antibody 8 in the reaction chamber 2.2 of described the first to the 3rd, the 5th to the 7th microtrabeculae type gel tube 2 respectively; The the 4th and the 8th microtrabeculae type gel tube 2 negative control tube are cavity in the reaction chamber 2.2 of the 4th and the 8th microtrabeculae type gel tube 2, as negative control.
Concrete detection method is as follows:
Collection of specimens: gather checked object venous blood, adding contains in the test tube of anti-coagulants.
The preparation of red blood cell sample: the sample of gathering is mixed with 0.5-0.8% red blood cell physiological saline suspension.
Experimental procedure:
(1) with eight microtrabeculae type gel tubes of ABO, RhD blood group antigens test card according to the 1-8 label;
(2) red blood cell sample to be checked is added respectively in first to the 3rd microtrabeculae type gel tube, or in the 4th to the 6th microtrabeculae type gel tube, every pipe 50 μ l;
(3) used special centrifugal machine at once centrifugal 5 minutes, 2 minutes 900rpm, 3 minutes 1500rpm take out back naked eyes result of determination.
The result judges:
Positive findings: the specific antigen-antibody compound that red cell antigens and corresponding antibodies form in micro-column gel floats in gel surface or the glue, positive reaction.
Response intensity: specificity red cell antigens antibody complex is positioned at the glue surface, is strong positive reaction; Compound is weak positive reaction in glue; More littler near glue bottom agglutinating particle, a little less than the reaction more.
Negative findings: tested red blood cell does not have corresponding antigen and antibodies, the specific antigen-antibody compound do not occur, and red blood cell is sunken to the point bottom of micro-column gel.
Claims (5)
1. an ABO, RhD blood group antigens test card, comprise fixed head (1), be provided with eight microtrabeculae type gel tubes (2) on the described fixed head (1) from left to right, each microtrabeculae type gel tube (2) includes convex body (2.1), reaction chamber (2.2) and gel separation post (2.3) from top to bottom, described convex body (2.1) is positioned at the top of fixed head (1) upper edge, and reaction chamber (2.2) and gel separation post (2.3) all are positioned at the below of fixed head (1) upper edge; All fill sephadex (5) in the gel separation post (2.3) of described eight microtrabeculae type gel tubes (2), it is characterized in that: first to the 3rd, be provided with anti-A mouse monoclonal antibody (6), anti-B mouse monoclonal antibody (7) and anti-D mouse monoclonal antibody (8) respectively in the reaction chamber (2.2) of the 5th to the 7th microtrabeculae type gel tube (2); In the reaction chamber (2.2) of the 4th and the 8th microtrabeculae type gel tube (2) is cavity.
2. a kind of ABO according to claim 1, RhD blood group antigens test card is characterized in that: fixed head (1) is the acrylic plastering transparent panel.
3. a kind of ABO according to claim 1 and 2, RhD blood group antigens test card is characterized in that: the outer wall of described each microtrabeculae type gel tube (2) is provided with two baffle plates (3), lays respectively at the front and back of fixed head (1).
4. a kind of ABO according to claim 1 and 2, RhD blood group antigens test card is characterized in that: the top of described fixed head (1) is provided with diaphragm (4), and diaphragm (4) covers on the convex body (2.1) of eight microtrabeculae type gel tubes (2).
5. a kind of ABO according to claim 3, RhD blood group antigens test card is characterized in that: the top of described fixed head (1) is provided with diaphragm (4), and diaphragm (4) covers on the convex body (2.1) of eight microtrabeculae type gel tubes (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220687920 CN203083997U (en) | 2012-12-13 | 2012-12-13 | ABO and RhD blood group antigen detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220687920 CN203083997U (en) | 2012-12-13 | 2012-12-13 | ABO and RhD blood group antigen detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203083997U true CN203083997U (en) | 2013-07-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220687920 Expired - Fee Related CN203083997U (en) | 2012-12-13 | 2012-12-13 | ABO and RhD blood group antigen detection card |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104991064A (en) * | 2015-06-27 | 2015-10-21 | 江阴金悦达生物技术有限公司 | Ovarian malignant tumor marker detection kit and preparation method thereof |
| CN105223366A (en) * | 2015-09-18 | 2016-01-06 | 深圳市达科为生物技术有限公司 | For the microtubule of Blood grouping, integral type ABO/RhD blood typing test card and preparation |
| CN105866444A (en) * | 2016-05-03 | 2016-08-17 | 江阴力博医药生物技术有限公司 | MNSs blood-group system detection reagent card and preparation method thereof |
| CN106290922A (en) * | 2016-09-20 | 2017-01-04 | 刘大基 | ABO is positive and negative and RhD antigens bloodtypes identifies combiner and authentication method |
| CN107202896A (en) * | 2017-05-26 | 2017-09-26 | 江苏力博医药生物技术股份有限公司 | A kind of quick detection IgG Subtypes reagent cards and preparation method thereof |
| CN110672862A (en) * | 2019-09-29 | 2020-01-10 | 迈克生物股份有限公司 | Blood type detection card and preparation method thereof |
-
2012
- 2012-12-13 CN CN 201220687920 patent/CN203083997U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104991064A (en) * | 2015-06-27 | 2015-10-21 | 江阴金悦达生物技术有限公司 | Ovarian malignant tumor marker detection kit and preparation method thereof |
| CN105223366A (en) * | 2015-09-18 | 2016-01-06 | 深圳市达科为生物技术有限公司 | For the microtubule of Blood grouping, integral type ABO/RhD blood typing test card and preparation |
| CN105866444A (en) * | 2016-05-03 | 2016-08-17 | 江阴力博医药生物技术有限公司 | MNSs blood-group system detection reagent card and preparation method thereof |
| CN106290922A (en) * | 2016-09-20 | 2017-01-04 | 刘大基 | ABO is positive and negative and RhD antigens bloodtypes identifies combiner and authentication method |
| CN107202896A (en) * | 2017-05-26 | 2017-09-26 | 江苏力博医药生物技术股份有限公司 | A kind of quick detection IgG Subtypes reagent cards and preparation method thereof |
| CN110672862A (en) * | 2019-09-29 | 2020-01-10 | 迈克生物股份有限公司 | Blood type detection card and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: JIANGSU FLON BIOTECHNOLOGY CO., LTD. Free format text: FORMER NAME: JIANGSU CHUANGSHENG BIOTECHNOLOGY CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: East Street Dongsheng Road Jiangyin 214434 Jiangsu province Wuxi City No. 78 Patentee after: Jiangsu Flon Biotechnology Co., Ltd. Address before: East Street Dongsheng Road Jiangyin 214434 Jiangsu province Wuxi City No. 78 Patentee before: Jiangsu Chuangsheng Biological Technology Co., Ltd. |
|
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Xi Weihong Inventor after: Wang Buqiang Inventor before: Xi Weihong Inventor before: Chen Yuping Inventor before: Wang Buqiang |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: XI WEIHONG CHEN YUPING WANG BUQIANG TO: XI WEIHONG WANG BUQIANG |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130724 Termination date: 20191213 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |