CN103149357B - A kind of Test paper card utilizing competition law to detect Brucella abortus antibody - Google Patents
A kind of Test paper card utilizing competition law to detect Brucella abortus antibody Download PDFInfo
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- CN103149357B CN103149357B CN201310033075.5A CN201310033075A CN103149357B CN 103149357 B CN103149357 B CN 103149357B CN 201310033075 A CN201310033075 A CN 201310033075A CN 103149357 B CN103149357 B CN 103149357B
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Abstract
The invention discloses a kind of Test paper card utilizing competition law to detect Brucella abortus antibody belonging to technical field of immunoassay.Test paper card described in this is made up of the test strips of shell and its inside, shell is made up of upper plate and lower plate, upper plate has detection window and well, test strips is made up of base plate and the absorption of sample pad pasted successively on base plate, colloid gold label pad, detection reaction district, absorption of sample pad is just to well, and detection reaction district is just to detection window.The invention also discloses the method applying above-mentioned ELISA test strip Brucella abortus antibody.Brucella abortus antibody field quick detection of the present invention test card sensitivity and repetition rate high, high specificity, stability is good, false positive rate and False-Negative Rate low, easy to use, easily observe difference, be suitable for clinical quick diagnosis and the epidemiology survey such as basic unit, rural area large-scale application.
Description
Technical field
The invention belongs to technical field of immunoassay, be specifically related to a kind of Test paper card utilizing competition law to detect Brucella abortus antibody.
Background technology
Brucella is Gram-negative bacillus pumilis, many in spherical during first separation, and club shape and oval, gradually in short and small shaft-like after this bacterium Secondary Culture, thalline atrichia, does not form brood cell.Within 1985, the WHO brucellosis Committee of Experts is divided into 6 kinds, 19 bions Brucella, i.e. sheep kind (bion 1 ~ 3), ox kind (bion 1 ~ 7.9), pig kind (bion 1 ~ 5) and sheep type epididymis kind, sarin mouse kind, kind of dog (each 1 bion).Maximum with sheep, ox, pig three kinds of meanings clinically, sheep kind pathogenicity is the strongest.The human diseases that brucella causes also has following several title: Malta fever, Mediterranean remittent fever, undulant fever or undulant fever.Bovine brucellosis extensively exists in China, pathogenicity is also very strong, and the northeast especially based on husbandry sector, North China, northwest one are with, and often have the report of Epidemic outbreak of disease, the health of direct harm humans, the serious puzzlement development of animal husbandry and the foreign trade of animals and animal product.
The diagnostic means of current Bovine brucellosis mainly comprises Antigen isolation and identification, rose bengal precipitation test (RBT), tube agglutination test (SAT), complement fixation test (CFT) (CFT) enzyme linked immunosorbent assay (ELISA), PCR etc.Antigen isolation and identification length consuming time, complex operation step, is suitable only for the research needs in laboratory, does not have practical value; Complement fixation test (CFT) easily causes hemolytic reaction, and the limitation of application is very large, and tube agglutination test is sxemiquantitative test method, easily occurs false positive reaction, and not easily detects the content of contained antibody in sample; Also there is higher false positive reaction in rose bengal precipitation test, needs to improve; Enzyme linked immunosorbent assay is highly sensitive, and quantitative effect is good, is a kind of immunological detection method of widespread use, but, the method operation is cumbersome, and quantitative and qualitative analysis needs the analysis of professional person to determine, is not suitable for detecting use on a large scale in the unit such as basic unit, rural area.
Immune colloidal gold technique, since appearance, has obtained and has developed rapidly.Be widely used in chemical detection and field of immunological detection, especially colloidal gold immunochromatographimethod technology have simple to operate, rapid sensitive, result are clear, be easy to judge and preserve, and without the need to advantages such as any instrument and equipments.Be more suitable for clinical quick diagnosis and the epidemiology survey such as basic unit, rural area large-scale application.
Summary of the invention
The object of the present invention is to provide that a Species sensitivity is high, specificity good, the competition law that utilizes that is simple to operate, low cost detects Brucella abortus antibody field quick detection test card and detection method thereof.
A kind of Test paper card utilizing competition law to detect Brucella abortus antibody, described Test paper card is made up of the test strips 2 of shell 1 and its inside, shell 1 is made up of upper plate 3 and lower plate 4, upper plate 3 has detection window 5 and well 6, test strips 2 is made up of base plate 7 and the absorption of sample pad 8 pasted successively on base plate 7, colloid gold label pad 9, detection reaction district 10, absorption of sample pad 8 is just to well 6, and detection reaction district 10 is just to detection window 5.
The material of described base plate 7 is nitrocellulose membrane.
Described colloid gold label pad 9 is coated with Brucella abortus antigen-colloid gold label thing.
The preparation method of described Brucella abortus antigen-colloid gold label thing is:
(1) by Brucella abortus antigen 4 DEG C of dialysed overnight in the NaCl solution of 0.005M/LpH7.0,4 DEG C, centrifugal 1h under 10000-12000rpm condition;
(2) pH of the colloidal gold solution of OD526 is adjusted to 4.0-9.0;
(3) borate buffer solution of centrifugal for step (1) the Brucella abortus antigen 0.005M/LpH9.0 obtained is diluted to 5 μ g/ml ~ 50 μ g/ml, the Brucella abortus antigen got after 1ml dilution joins in 1ml colloidal gold solution, vibration mixing, leave standstill 5min, add the NaCl solution of 0.1ml10%, leave standstill 2h after mixing, centrifugal purification obtains the Brucella abortus antigen of colloid gold label.
Described detection reaction district 10 is made up of by the quality control region C that Brucella abortus two resists by the test section T of Brucella abortus antibody and bag bag.
Described absorption of sample pad contains phytolectin or anti erythrocyte antibody.
Described anti erythrocyte antibody is end user's erythrocyte immune mouse, sheep or rabbit, the anti-erythrocyte monoclonal antibody of conventional preparation.
The detection method of the Test paper card of above-mentioned detection Brucella abortus antibody, is characterized in that, carry out in accordance with the following steps:
(1) blood sample process: blood specimen collection is in clean, dry container, and the new blood of collection being added concentration is 2.5% anti-coagulants;
(2) treated blood sample 100 μ L is got, instillation well;
(3) treat that blood sample flows through test section and quality control region, judge that decision rule is as follows whether containing Brucella abortus antibody in sample:
Positive (-): only an aubergine band appears in quality control region, occurs in test section without aubergine band;
Negative (+): two aubergine bands occur, one is positioned at test section, and another is positioned at quality control region;
Invalid: when quality control region does not demonstrate aubergine band, then no matter whether test section demonstrates aubergine band, and it is invalid that this test card is judged to.
Cleaning Principle of the present invention: there are letter " T " and " C " in B rucella melitensis antibody Rapid detection test strip surface as detection line and nature controlling line.With the conjugate of Brucella antigen and colloid gold particle, be coated in cellulose acetate membrane; B rucella melitensis antibody and two are anti-ly coated on test section T and quality control region C by nitrocellulose filter respectively.Before providing any sample, detection line and nature controlling line do not show in results window.After serum sample is added to well, the chromatography swimming together with antigen-Gold Conjugate A of the B rucella melitensis antibody in sample is competed to detection zone and is combined with B rucella melitensis antibody.When the B rucella melitensis antibody concentration in sample exceedes a certain amount of, Brucella antigen-Gold Conjugate A just can not be combined by the B rucella melitensis antibody on detection line, and now aubergine lines do not appear in detection zone; When not having in sample that in B rucella melitensis antibody or sample, B rucella melitensis antibody concentration is lower than certain value, the B rucella melitensis antibody of Gold Conjugate A just on detection line is combined, thus demonstrates aubergine lines in detection zone; And no matter in sample whether containing B rucella melitensis antibody, all can there are aubergine lines in quality control region, detect effectively to show.
Beneficial effect of the present invention: Brucella abortus antibody field quick detection of the present invention test card sensitivity and repetition rate high, high specificity, stability is good, false positive rate and False-Negative Rate control below 1%, the phytolectin that absorption pad contains or anti erythrocyte antibody effective aggegation can tackle red blood cell, avoid red blood cell through absorption pad, the detection of interfere with subsequent, this test card detection reaction easily observes difference, easy to use, easy observation difference, is suitable for clinical quick diagnosis and the epidemiology survey such as basic unit, rural area large-scale application.
Accompanying drawing explanation
Fig. 1 is test card structural representation of the present invention;
Fig. 2 is test strips structural representation of the present invention;
In figure, 1-shell, 2-test strips, 3-upper plate, 4-lower plate, 5-detection window, 6-well, 7-base plate 8-absorption of sample pad, 9-colloid gold label pad, 10-detection reaction district, T-test section, C-quality control region.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
Embodiment 1 utilizes sandwich method to detect the Test paper card structure of Brucella abortus antibody
Sandwich method is utilized to detect the Test paper card of Brucella abortus antibody, as depicted in figs. 1 and 2, described Test paper card is made up of the test strips 2 of shell 1 and its inside, shell 1 is made up of upper plate 3 and lower plate 4, upper plate 3 has detection window 5 and well 6, test strips 2 is made up of base plate 7 and the absorption of sample pad 8 pasted successively on base plate 7, colloid gold label pad 9, detection reaction district 10, and absorption of sample pad 8 is just to well 6, and detection reaction district 10 is just to detection window 5.Colloid gold label pad 9 is coated with brucellergen and colloid gold label thing.Detection reaction district 10 is made up of by the quality control region C that brucella two resists by the test section T of Brucella antibody and bag bag.
Absorption of sample pad contains phytolectin or anti erythrocyte antibody.
Anti erythrocyte antibody is end user's erythrocyte immune mouse, sheep or rabbit, the anti-erythrocyte monoclonal antibody of conventional preparation.
Embodiment 2 detects the method for Brucella abortus antibody
1, the preparation of Brucella abortus antigen-colloid gold label thing
(1) by Brucella abortus antigen 4 DEG C of dialysed overnight in the NaCl solution of 0.005M/LpH7.0,4 DEG C, centrifugal 1h under 10000-12000rpm condition;
(2) pH of the colloidal gold solution of OD526 is adjusted to 4.0-9.0;
(3) borate buffer solution of centrifugal for step (1) the Brucella abortus antigen 0.005M/LpH9.0 obtained is diluted to 5 μ g/ml ~ 50 μ g/ml, the Brucella abortus antigen got after 1ml dilution joins in 1ml colloidal gold solution, vibration mixing, leave standstill 5min, add the NaCl solution of 0.1ml10%, leave standstill 2h after mixing, centrifugal purification obtains the Brucella abortus antigen of colloid gold label.
2, the process of blood sample
Get 100 parts of bovine blood samples through Brucella abortus antigen immune, blood specimen collection is in clean, dry container, and the new blood of collection being added concentration is 2.5% anti-coagulants; Blood preparation can preserve 48 hours 2 DEG C ~ 8 DEG C refrigerations.
3, detecting step
(1) by 100 parts of each 100 μ L of treated blood sample, standard Brucella abortus antibody 100 μ L (concentration is 10 μ g/ml), deionized water 100 μ L, instillation well;
(2) treat that blood sample, standard Brucella abortus antibody, deionized water flow through test section and the quality control region of respective test strips, judge that decision rule is as follows whether containing Brucella abortus antibody in sample:
Positive (-): only an aubergine band appears in quality control region, occurs in test section without aubergine band;
Negative (+): two aubergine bands occur, one is positioned at test section, and another is positioned at quality control region;
Invalid: when quality control region does not demonstrate aubergine band, then no matter whether test section demonstrates aubergine band, and it is invalid that this test card is judged to.
Result of determination is: 98 parts, bovine blood sample is positive, and 1 part is negative, and 1 part invalid, and sensitivity is 99%.
Embodiment 3 detects test card false positive rate and the false negative test experiments of Brucella abortus antibody
1, false positive test
(1) get 100 parts of common bovine blood samples, adopt blood pretreating device to process blood sample;
(2) by each 100 μ L of treated 100 parts of common bovine blood samples, deionized water 100 μ L, instillation well;
(2) treat blood sample, test section and quality control region that deionized water flows through respective test strips, judge that decision rule is as follows whether containing Brucella abortus antibody in sample:
Positive (-): only an aubergine band appears in quality control region, occurs in test section without aubergine band;
Negative (+): two aubergine bands occur, one is positioned at test section, and another is positioned at quality control region;
Invalid: when quality control region does not demonstrate aubergine band, then no matter whether test section demonstrates aubergine band, and it is invalid that this test card is judged to.
Result of determination is: 1 part, bovine blood sample is positive, and 99 parts are negative, and all effectively, false positive rate is 1%.
2, false negative test
(1) 100 parts of standard Brucella abortus antibody (concentration is 10 μ g/ml) each 100 μ L, deionized water 100 μ L is got, instillation well;
(2) treat standard Brucella abortus antibody, test section and quality control region that deionized water flows through respective test strips, judge that decision rule is as follows whether containing Brucella abortus antibody in sample:
Positive (-): only an aubergine band appears in quality control region, occurs in test section without aubergine band;
Negative (+): two aubergine bands occur, one is positioned at test section, and another is positioned at quality control region;
Invalid: when quality control region does not demonstrate aubergine band, then no matter whether test section demonstrates aubergine band, and it is invalid that this test card is judged to.
Result of determination is: 99 parts, bovine blood sample is positive, and 1 part is negative, and all effectively, false negative rate is 1%.
Embodiment 4 detects the mensuration of other performances of test strips of Brucella abortus antibody
(1) specific assay of Brucella antibody field quick detection test card
The Brucella antibody test card of application preparation detects the 3 kind bacterium whole bloods nearer with brucella germline, and its result is feminine gender, shows that its specificity is good, without intersecting.
(2) susceptibility of Brucella antibody field quick detection test card
Get outsourcing Brucella abortus positive serum, detect its mean titre with ELISA and be about 1: 8000, brave red Avian tubercula plain agglutination test antigen at hemodilution degree lower than test positive when 50 times; It is 1: 6000 that the blood of the positive findings that Brucella antibody field quick detection test card measures is tired, and during hemodilution degree >=6000 times, the colour developing of test card detection line is unintelligible or without colour developing.Therefore the sensitivity that Brucella antibody field quick detection is blocked really is apparently higher than rose bengal precipitation test, but its sensitivity is still lower than ELISA.
(3) repeatability of Brucella antibody field quick detection test card
Carry out Parallel testing 5 test cards, its testing result is consistent, illustrates that this detection method has repeatability.
(4) stability of Brucella antibody field quick detection test card
Place Brucella antibody field quick detection test cards, periodic detection standard positive serum for 4 DEG C, result shows its stability can preserve 1 year, and after 1 year, golden release rate slows down, and Tomography Velocity such as to slow down at the phenomenon.The stability of product is 4 DEG C of environment is 1 year.
Claims (1)
1. the Test paper card utilizing competition law to detect Brucella abortus antibody, it is characterized in that, described Test paper card is made up of the test strips (2) of shell (1) and its inside, shell (1) is made up of upper plate (3) and lower plate (4), upper plate (3) has detection window (5) and well (6), test strips (2) is by base plate (7) and the absorption of sample pad (8) pasted successively on base plate (7), colloid gold label pad (9), detection reaction district (10) is formed, absorption of sample pad (8) is just to well (6), detection reaction district (10) is just to detection window (5),
The material of described base plate (7) is nitrocellulose membrane;
Described colloid gold label pad (9) is coated with Brucella abortus antigen-colloid gold label thing;
The preparation method of described Brucella abortus antigen-colloid gold label thing is:
(1) by Brucella abortus antigen 4 DEG C of dialysed overnight in the NaCl solution of 0.005M/LpH7.0,4 DEG C, centrifugal 1h under 10000-12000rpm condition;
(2) pH of the colloidal gold solution of OD526 is adjusted to 4.0-9.0;
(3) borate buffer solution of centrifugal for step (1) the Brucella abortus antigen 0.005M/LpH9.0 obtained is diluted to 5 μ g/ml ~ 50 μ g/ml, the Brucella abortus antigen got after 1ml dilution joins in 1ml colloidal gold solution, vibration mixing, leave standstill 5min, add the NaCl solution of 0.1ml10%, leave standstill 2h after mixing, centrifugal purification obtains the Brucella abortus antigen of colloid gold label;
Described detection reaction district (10) is made up of by the quality control region of Brucella abortus primary antibodie (C) by the test section of Brucella abortus antibody (T) and bag bag;
Described absorption of sample pad contains phytolectin or anti erythrocyte antibody;
Described anti erythrocyte antibody is end user's erythrocyte immune mouse, sheep or rabbit, the anti-erythrocyte monoclonal antibody of conventional preparation;
The detection method of above-mentioned Test paper card, carry out in accordance with the following steps:
(1) blood sample process: blood specimen collection is in clean, dry container, and the new blood of collection being added concentration is 2.5% anti-coagulants;
(2) treated blood sample 100 μ L is got, instillation well;
(3) treat that blood sample flows through test section and quality control region, judge that decision rule is as follows whether containing Brucella abortus antibody in sample:
Positive (+): only an aubergine band appears in quality control region, occurs in test section without aubergine band;
Negative (-): two aubergine bands occur, one is positioned at test section, and another is positioned at quality control region;
Invalid: when quality control region does not demonstrate aubergine band, then no matter whether test section demonstrates aubergine band, and it is invalid that this test card is judged to.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104090100B (en) * | 2014-07-16 | 2016-05-18 | 李克生 | Brucella antibody gold labeled quick detection reagent box |
| CN104749362A (en) * | 2015-03-09 | 2015-07-01 | 中国农业科学院特产研究所 | Deer brucellosis colloidal gold antibody detection test paper strip and preparation method thereof |
| CN105929154A (en) * | 2016-04-22 | 2016-09-07 | 深圳真瑞生物科技有限公司 | Test strip and kit for rapid detection of antibody against brucellosis |
| CN115166238A (en) * | 2022-08-02 | 2022-10-11 | 中国农业科学院北京畜牧兽医研究所 | Integrated antibody detection test strip for primary screening and accurate diagnosis of brucellosis in sheep |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363859A (en) * | 2008-05-26 | 2009-02-11 | 北京庄笛浩禾生物医学科技有限公司 | Test paper strip for rapidly detecting brucellosis antibody |
| CN101441215A (en) * | 2008-12-31 | 2009-05-27 | 吉林大学 | Brucella antibody immune colloidal gold detection test paper strip and preparing method thereof |
| CN101520458A (en) * | 2008-02-25 | 2009-09-02 | 广州天美生物技术有限公司 | Simple method for detecting HLA-G and antibody thereof by gold-labeled immunoassay |
| CN101592661A (en) * | 2009-03-26 | 2009-12-02 | 张晓艳 | The brucellosis antibody competition enzyme-linked immunosorbent adsorption test detection kit |
| CN101620226A (en) * | 2009-08-07 | 2010-01-06 | 四川省迈克科技有限责任公司 | Quick test kit of schistosomiasis and preparation method thereof |
| EP2272860A1 (en) * | 2009-07-10 | 2011-01-12 | Institut Pasteur | Multivalent epitope in complex with a detection marker for the early serodiagnosis of infections |
-
2013
- 2013-01-29 CN CN201310033075.5A patent/CN103149357B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101520458A (en) * | 2008-02-25 | 2009-09-02 | 广州天美生物技术有限公司 | Simple method for detecting HLA-G and antibody thereof by gold-labeled immunoassay |
| CN101363859A (en) * | 2008-05-26 | 2009-02-11 | 北京庄笛浩禾生物医学科技有限公司 | Test paper strip for rapidly detecting brucellosis antibody |
| CN101441215A (en) * | 2008-12-31 | 2009-05-27 | 吉林大学 | Brucella antibody immune colloidal gold detection test paper strip and preparing method thereof |
| CN101592661A (en) * | 2009-03-26 | 2009-12-02 | 张晓艳 | The brucellosis antibody competition enzyme-linked immunosorbent adsorption test detection kit |
| EP2272860A1 (en) * | 2009-07-10 | 2011-01-12 | Institut Pasteur | Multivalent epitope in complex with a detection marker for the early serodiagnosis of infections |
| CN101620226A (en) * | 2009-08-07 | 2010-01-06 | 四川省迈克科技有限责任公司 | Quick test kit of schistosomiasis and preparation method thereof |
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