CN101620226A - Quick test kit of schistosomiasis and preparation method thereof - Google Patents
Quick test kit of schistosomiasis and preparation method thereof Download PDFInfo
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- CN101620226A CN101620226A CN 200910060300 CN200910060300A CN101620226A CN 101620226 A CN101620226 A CN 101620226A CN 200910060300 CN200910060300 CN 200910060300 CN 200910060300 A CN200910060300 A CN 200910060300A CN 101620226 A CN101620226 A CN 101620226A
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Abstract
The invention discloses a quick test kit of schistosomiasis and a preparation method thereof. A water absorbing pad, a pyroxylin film, an aerosol bonding pad and a sample pad are and sequentially connected in horizontal direction and fixed on a baseplate which is arranged in a kit body, and the kit body is provided with an observation window corresponding to the pyroxylin film and a sample loading hole corresponding to the sample pad. Only an one-step operation is needed when the quick test kit is used, and the quick test kit not only can detect serum and plasma samples, but also can detect a whole blood sample, thereby avoiding the step of obtaining the serum sample which can be obtained only through a centrifugation step from a blood sample, and greatly improving the screening speed. The quick test kit can also detect whether animals such as cows, horses, pigs, rabbits, and the like have the schistosomiasis or not so as to overcome the defect that different kits are needed to be prepared for detecting different species of other kits.
Description
Technical field
The present invention relates to a kind of quick test kit of schistosomiasis, need not special instrument and technical professional and just can finish mensuration.
The invention still further relates to a kind of preparation method of quick test kit of schistosomiasis, avoided the loaded down with trivial details operation of classic method, make things convenient for field quick detection.
Background technology
Snail fever is the parasitic disease of pandemic a kind of serious harm people, animal health in the world wide, its susceptible animal comprises domestic animal and wild animals such as people, pig, rabbit, ox, sheep, domestic animal also is the topmost infection sources of human body snail fever, and it is the snail fever people at highest risk that the whole world has 600,000,000 populations approximately.China is by the comprehensive regulation, and epidemic situation was once controlled, but the incidence of disease is in rising trend again in recent years, simultaneously still has the task of long-term consolidation and monitoring in the area of elimination schistosoma disease.China has 6,500 ten thousand people to live in the epidemic-stricken area approximately at present, and the chronic schistosomiasis people estimates more than 1,000,000.Behind the schistosoma japonicum infection host, the host can produce specific antibody in week at 2-4, and snail fever is after curing, and the antibody in the host still can exist and last very long.Detect the just infected or previous infection of schistosome antibody explanation patient, testing result has important etiological diagnosis meaning.Show that according to statistics people and animals' schistosomiasis infection rate in most of popular district is at 3%-5%, some areas people and animals' schistosomiasis infection rate can be up to 68%.
The detection method that snail fever is traditional is aetology detections such as excrement inspection, needs to drop into bigger human and material resources and fund.Main use is immunology detection now, as circum oval precipitating test, indirect hemagglutination test, enzyme linked immunosorbent assay, dot-ELISA etc., but certain defective is arranged all, required detection time is long, generally need 40-240 minute, that have even reach 72 hours, need instrument and equipment, be not suitable for extensively promoting the use of at the scene.Chinese patent 200510050188.1 provides a kind of kit and detection method of diagnosing snail fever, this method detects step: need take blood sample to drip in paper during detection, cutting dry blood paper behind the airing soaks a period of time in physiological saline, the blood sample leachate that obtains is drawn back point sample on nitrocellulose filter with the glass capillary, on nitrocellulose filter, drip the schistosome antigen collaurum again after placing a period of time under the room temperature, it is positive that the point sample place shows punctation, otherwise negative.This method shortcoming is: when detecting, not only need other instrument and equipment, and complex operation step, waste time and energy.
In current prevention and cure of snail fever work, press for a kind of quick, easy, accurate, can be used for the on-the-spot instrument that detects, to improve on-the-spot large-scale examination speed.
Summary of the invention
The purpose of this invention is to provide a kind of blood fluke fast measuring kit, only need single stepping, saved blood sample and will pass through the step that centrifugation step just can obtain serum sample, improved the speed of examination greatly.
Another object of the present invention provides a kind of preparation method of blood fluke fast measuring kit, solve that the traditional detection consumptive material is many, complex operation, detection time are grown, to the demanding problem of condition of work.
For blood fluke fast measuring kit, the present invention solve the technical problem the technical scheme that is adopted and is:
Quick test kit of schistosomiasis comprises base plate, also comprises nitrocellulose filter, collaurum pad, sample pad and adsorptive pads, and described adsorptive pads, nitrocellulose filter, collaurum pad and sample pad horizontal direction are joined in proper order and be fixed on the base plate.The horizontal direction connected mode has two kinds, and a kind of is that horizontal direction connects from beginning to end, and a kind of is the connection of overlapping of horizontal direction.
An optimal way of the present invention is that aforesaid blood fluke fast measuring kit also comprises box body, and base plate places in the box body, and the position corresponding to nitrocellulose filter on the described box body box face is provided with view window, is provided with well corresponding to the position of sample pad.Described view window is square opening or slide.
Another optimal way of the present invention is aforesaid blood fluke fast measuring kit, and the adsorptive pads top is provided with the Product labelling layer, and collaurum pad top is provided with the arrow label layer.
Principle of work of the present invention is: japonice ovum antigen is fixed on the nitrocellulose filter as detection line with ribbon, the colloidal gold particle that is marked with second antibody is adsorbed on the collaurum pad, after testing sample (blood) is added on the sample pad, aggegation is taking place and is being fixed on the sample pad in red blood cell on the sample pad, plasma sample moves forward by capillary action, mix and react to each other with the colloid gold label reagent on the collaurum pad, continue to move forward when containing corresponding detection antigen regional on the nitrocellulose filter, if blood sample contains schistosome antibody, the colloid gold label reagent complex combines and is trapped with antigen generation specificity, and accumulating in develops the color on the detection line is positive; If sample does not contain schistosome antibody, specificity does not take place with antigen and combines in the collaurum albumen composition, does not develop the color on the detection line and is negative.
For the preparation method of above-mentioned quick test kit of schistosomiasis, the technical scheme that the present invention solve the technical problem employing is:
Comprise the steps:
1) preparation of sample pad:
With concentration is phytolectin or the anti-human erythrocyte antibody buffer solution pack processing suppressed by vector of 0.5mg/ml-1mg/ml, and low-temperature freeze-dry cuts into rectangular standby;
2) preparation of collaurum pad:
A. the preparation of colloidal gold solution: with purified water, 1% chlorauric acid solution and 1% sodium citrate solution is raw material, wherein 1% chlorauric acid solution equates with the volume of 1% sodium citrate solution, earlier with the purified water heated and stirred, add again 1% chlorauric acid solution make the final concentration of gold chloride be ten thousand/, when treating that solution is little and boiling, add 1% sodium citrate solution fast, continue heating and stirring, stop heating after 5-10 minute, solution is cooled to room temperature, obtain the colloidal gold solution of mean diameter at 40nm-50nm, 4 ℃ of preservations are standby;
B. the adjusting of collaurum pH: the colloidal gold solution that above-mentioned steps obtains is regulated pH at 6-8;
C. colloid gold label: add albumen to be marked in the colloidal gold solution that above-mentioned steps obtains, stir after 10-30 minute, the bovine serum albumin(BSA) that adds 10ml 10% in the every 100ml solution that obtains, 8000-12000rpm is centrifugal 30 minutes behind the mixing, abandon supernatant, precipitate the treated collaurum albumen composition that obtains;
D. the preparation of collaurum pad: the collaurum albumen composition that above-mentioned steps obtains is layered on the pad uniformly, and low-temperature freeze-dry cuts into rectangular standby;
3) preparation of japonice ovum antigen:
After the japonice ovum antigen grinding, in the normal saline solution of adding 0.85%, place-20 ℃ of freeze overnight, the dissolving on daytime, so multigelation is placed on for 5-10 time in the rubble ice, uses ultrasonic disruption 3 minutes, broken 10 times repeatedly, 12000rpm high speed centrifugation 30 minutes is got supernatant and is japonice ovum antigen;
4) nitrocellulose filter is provided with detection line and nature controlling line: the anti-human IgG bag that will be drawn into the 1mg/ml japonice ovum antigen in the detection line syringe pump by machine and be drawn into 1mg/ml in the nature controlling line syringe pump with bag is formed detection line and nature controlling line respectively to nitrocellulose filter, standby after 37 ℃ of dried overnight.
Described precipitation is to handle like this to obtain the collaurum albumen composition: precipitation is according to the ratio of 100ml: 10ml, and every 100ml collaurum suspends with the PBS solution that 10ml contains 1% bovine serum albumin(BSA), 0.1% polyglycol, pH=8.2.
Described sample pad adopts glass fibre membrane or nonwoven fabrics as the bag suppressed by vector, and the collaurum pad adopts glass fibre membrane, nonwoven fabrics or polyester film as the bag suppressed by vector.
Described nitrocellulose filter adopts nylon membrane, carboxylation cellulose membrane or pure cellulose film to substitute.
The detection method of quick test kit of schistosomiasis of the present invention is operated according to following steps: sample to be tested is directly joined in the well of kit, re-use dropper dropping 2-3 and drip dilution in well, in 15 minutes in the view window detection line colour developing promptly positive, detection line does not develop the color negative.
The invention has the beneficial effects as follows:
(1) the present invention is layered on adsorptive pads, nitrocellulose filter, collaurum pad and sample pad on the base plate in proper order, and volume is little, and is easy to make; Place in the box body, volume is little, be easy to carry again;
(2) box body is provided with the well that enters for sample and for the view window of observed result, according to the judgement testing result that whether develops the color, accurately and reliably, does not have artificial operate miss;
(3) snail fever shortens detection time greatly, have fast, simple, on-the-spot, do not need expensive instrument, do not need personnel are carried out advantage such as professional training;
(4) the present invention is marked at colloidal gold particle on the capture antibody, does not have the participation of objectionable impurities and radiomaterial, and is safe and reliable, is applicable to the diagnosis of snail fever;
(5) be not only applicable to whether exist in the human body blood sample schistosome antibody, whether the blood sample that can also detect animals such as ox, horse, pig, rabbit exists schistosome antibody, has overcome other kits and has detected the shortcoming that different genera also needs to prepare different kits;
(6) can produce in batches, can single single part, also can many people single part, be applicable to the fast detecting of clinical quick diagnosis and on-the-spot big quantity; Be easy to preserve, help grass-roots unit and promote.
Description of drawings
Fig. 1 is embodiment 1 sectional view;
Fig. 2 is embodiment 2 sectional views;
Fig. 3 is embodiment 3 sectional views;
Fig. 4 is embodiment 4 cut-open views.
The corresponding component names of mark among the figure: 1-base plate; The 2-nitrocellulose filter; 3-collaurum pad; The 4-sample pad; The 5-adsorptive pads; 6-Product labelling layer; 7-arrow label layer; The 8-view window; The 9-well; The 10-box body.
Embodiment
As shown in Figure 1, quick test kit of schistosomiasis, comprise base plate 1, also comprise nitrocellulose filter 2, collaurum pad 3, sample pad 4 and adsorptive pads 5, described adsorptive pads 5, nitrocellulose filter 2, collaurum pad 3 and sample pad 4 horizontal directions are joined in proper order and are fixed on the base plate 1, concrete, its connected mode is that horizontal direction connects from beginning to end.Adsorptive pads 5 tops are provided with Product labelling layer 6, and collaurum pad 3 tops are provided with arrow label layer 7.
As shown in Figure 2, quick test kit of schistosomiasis, comprise base plate 1, nitrocellulose filter 2, collaurum pad 3, sample pad 4 and adsorptive pads 5, adsorptive pads 5, nitrocellulose filter 2, collaurum pad 3 and sample pad 4 horizontal directions are joined in proper order and are fixed on the base plate 1, concrete, its connected mode is that horizontal direction connects from beginning to end.Also comprise box body 10, base plate 1 places in the box body 10, and the position corresponding to nitrocellulose filter 2 on the described box body 10 box faces is provided with view window 8, is provided with well 9 corresponding to the position of sample pad 4.
As shown in Figure 3, described adsorptive pads 5, nitrocellulose filter 2, collaurum pad 3 and sample pad 4 horizontal directions are joined in proper order and are fixed on the base plate 1, and are concrete, the connection of overlapping of its connected mode.All the other are identical with embodiment 1.
As shown in Figure 4, described adsorptive pads 5, nitrocellulose filter 2, collaurum pad 3 and sample pad 4 horizontal directions are joined in proper order and are fixed on the base plate 1, and are concrete, the connection of overlapping of its connected mode.All the other are identical with embodiment 2.
The preparation method of quick test kit of schistosomiasis is as follows:
(1) preparation of sample pad
To contain anti-human erythrocyte antibody or the phytolectin buffer solution uniform treatment glass fibre membrane that concentration is 0.5mg/ml-1mg/ml or to spin cloth bag suppressed by vector, bag can be taked will wrap by solution with pipettor by mode and be taped against uniformly in the sample pad, also can take to place bag to be soaked evenly by solution sample pad.The capsulating material of handling well adopts the mode of freeze drying, vacuum drying or heat drying to handle, and cuts into the rectangular back standby of suitable size according to specification again.
(2) preparation colloidal gold solution
The material of collaurum pad adopts glass fibre membrane, nonwoven fabrics or polyester film as the bag suppressed by vector.
Adopt trisodium citrate reduction method to prepare the nano-colloid gold solution.Add the 1L purified water in the clean round-bottomed flask, heating is also stirred, get 1% chlorauric acid solution 10ml in round-bottomed flask, when treating the solution boiling, the citric acid three sodium solution 10ml of adding 1% continues agitating solution and also reacted 5-10 minute fast, and the gold chloride in the solution is reduced into gold grain gradually, solution to black, becomes claret by colourless at last.2-8 ℃ of preservation is standby after stopping heating solution being cooled to room temperature.
(3) best pH of colloid gold label and optimum mark protein content
Before colloid gold label protein, to carry out determining of optimum mark PH and labelled protein amount earlier, reach with minimum albumen consumption and collaurum and reach the best ratio that combines, both can save the consumption of albumen, produce too much free label albumen after can reducing mark again, simplify purifying workload by centrifugal removal floating preteins.This test method adopts ocular estimate, gets a series of centrifuge tubes, adds the 1ml colloidal gold solution in every pipe, specifically according to table handling down:
Table 1 collaurum-SPA optimum mark pH determines
| Test tube number | ??1 | ??2 | ??3 | ??4 | ??5 | ??6 |
| Collaurum | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml |
| Mark pH | ??5.5 | ??6.0 | ??6.5 | ??7.0 | ??7.5 | ??8.0 |
| Protein content | ??30μg | ??30μg | ??30μg | ??30μg | ??30μg | ?? |
| 10% sodium chloride | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml |
| Solution colour changes | Red-purple | Red-red | Red-red | Red-red | Red-red | Red-red |
Determining of table 2 collaurum-SPA optimum mark protein content
| Test tube number | ??1 | ??2 | ??3 | ??4 | ??5 | ??6 |
| Collaurum | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml |
| Mark pH | ??6.0 | ??6.0 | ??6.0 | ??6.0 | ??6.0 | ??6.0 |
| Protein content | ??2μg | ??4μg | ??6μg | ??8μg | ??10μg | ??12μg |
| 10% sodium chloride | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml |
| Solution colour changes | Red-purple | Red-purple | Red-purple | Red-red | Red-red | Red-red |
Change as seen from the solution colour of collaurum, can keep the stable PH of colloid-SPA to be best pH, the best PH of colloid gold label SPA is 6.0, colloid gold label SPA optimum mark protein content adds 10%-20% on the basis for minimum mark protein content that can the stable colloid gold again, as seen, the optimum mark protein content of colloid gold label SPA is 10ug.
Determining of best PH of colloid gold label mouse-anti human IgG and protein content
Get a series of centrifuge tubes, add the 1ml colloidal gold solution in every pipe, specifically according to table handling down:
Table 1 collaurum-mouse-anti human IgG optimum mark pH determines
| Test tube number | ??1 | ??2 | ??3 | ??4 | ??5 | ??6 |
| Collaurum | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml |
| Mark P H | ??5.5 | ??6.0 | ??6.5 | ??7.0 | ??7.5 | ??8.0 |
| Protein content | ??30μg | ??30μg | ??30μg | ??30μg | ??30μg | ??30μg |
| 10% sodium chloride | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml |
| Solution colour changes | Red-purple | Red-purple | Red-purple | Red-purple | Red-red | Red-red |
Determining of table 2 collaurum-mouse-anti human IgG optimum mark protein content
| Test tube number | ??1 | ??2 | ??3 | ??4 | ??5 | ??6 |
| Collaurum | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml | ??1ml |
| Mark P H | ??7.5 | ??7.5 | ??7.5 | ??7.5 | ??7.5 | ??7.5 |
| Protein content | ??2μg | ??4μg | ??6μg | ??8μg | ??10μg | ??12μg |
| 10% sodium chloride | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml | ??0.1ml |
| Solution colour changes | Red-purple | Red-purple | Red-purple | Red-red | Red-red | Red-red |
Change as seen from the solution colour of collaurum, can keep colloid-mouse-anti human IgG stable p H to be best pH, the best pH of colloid gold label mouse-anti human IgG is 7.5, body gold mark mouse-anti human IgG optimum mark protein content adds 10%-20% on the basis for minimum mark protein content that can the stable colloid gold again, as seen, the optimum mark protein content of colloid gold label SPA is 10ug.
The capture antibody that adds the optimum mark protein content in the colloidal gold solution, as goat anti-human igg, mouse-anti human IgG, SPA, SPG albumen, stir after 10-30 minute, add 10% bovine serum albumin(BSA) or add 1% polyglycol according to the ratio of every 100ml solution 10ml according to the ratio of every 100ml solution 10ml, behind the mixing 8000-12000 rev/min centrifugal 30 minutes, abandon supernatant, red precipitate suspends with the PBS solution that contains 1% bovine serum albumin(BSA), 0.1% polyglycol, pH=8.2 according to the ratio of every 100ml collaurum 10ml.
(4) preparation of collaurum pad
The collaurum albumen composition that above-mentioned steps obtains is layered on the pad uniformly.Adopted the mode of freeze drying, vacuum drying or heat drying to handle by good collaurum pad bag, cut into the rectangular standby of suitable size according to specification again.
(5) the bag quilt of nitrocellulose filter
After the japonice ovum antigen grinding, the normal saline solution with 0.85% places-20 ℃ of freeze overnight, the dissolving on daytime, and so multigelation is 5-10 time, and solution is placed rubble ice, uses ultrasonic disruption 3 minutes, broken 10 times repeatedly.12000 rev/mins of high speed centrifugations 30 minutes are got supernatant and are japonice ovum antigen.
The japonice ovum antigen of 1mg/ml is drawn in the detection line syringe pump, the anti-human IgG of 1mg/ml is drawn in the nature controlling line syringe pump, with bag by machine according to the bag of 0.5-1 μ l/cm by speed, japonice ovum antigen and anti-human IgG bag are arrived the suitable position of nitrocellulose filter, standby after 37 ℃ of dried overnight.
Described nitrocellulose filter can be by nylon membrane or carboxylation cellulose membrane or pure cellulose film material as an alternative.
6) preparation of quick test kit of schistosomiasis
Join in proper order and paste on the base plate 1 with adsorptive pads 5 and according to nitrocellulose filter 2, collaurum pad 3 and sample pad 4 horizontal directions that above-mentioned steps prepares, on collaurum pad 3, paste arrow label layer 7, Paste Product label layer 6 above adsorptive pads 5, the slitting width is 3mm, obtain semi-manufacture, one person-portion semi-manufacture and a person-portion drying agent are packed in the aluminium plastic bag, again the heat-sealing of aluminium plastic bag openend is got final product.
The detecting operation program: the whole blood sample of getting the patient is in the test tube of cleaning, adopt micropipettor or quantitative capillary to draw 1 μ l whole blood sample, serum or plasma sample, sample to be tested is directly joined in the well, re-use dropper dropping 2-3 and drip dilution in the well of kit.Whether observe detection line after 10-15 minute and develop the color, the detection line colour developing is promptly positive, detection line does not develop the color negative.
Claims (8)
1, quick test kit of schistosomiasis, comprise base plate (1), it is characterized in that, also comprise nitrocellulose filter (2), collaurum pad (3), sample pad (4) and adsorptive pads (5), described adsorptive pads (5), nitrocellulose filter (2), collaurum pad (3) and sample pad (4) horizontal direction are joined in proper order and are fixed on the base plate (1).
2, quick test kit of schistosomiasis according to claim 1, it is characterized in that, also comprise box body (10), base plate (1) places in the box body (10), position corresponding to nitrocellulose filter (2) on described box body (10) the box face is provided with view window (8), is provided with well (9) corresponding to the position of sample pad (4).
3, quick test kit of schistosomiasis according to claim 2 is characterized in that, described view window (8) is square opening or slide.
4, quick test kit of schistosomiasis according to claim 1, it is characterized in that, described adsorptive pads (5) top is provided with it the directly Product labelling layer (6) of contact, and collaurum pad (3) top is provided with it the directly arrow label layer (7) of contact.
5, a kind of preparation method of quick test kit of schistosomiasis as claimed in claim 1 is characterized in that, comprises the steps:
1) preparation of sample pad:
With concentration is phytolectin or the anti-human erythrocyte antibody buffer solution pack processing suppressed by vector of 0.5mg/ml-1mg/ml, and low-temperature freeze-dry cuts into rectangular standby;
2) preparation of collaurum pad:
A. the preparation of colloidal gold solution: with purified water, 1% chlorauric acid solution and 1% sodium citrate solution is raw material, wherein 1% chlorauric acid solution equates with the volume of 1% sodium citrate solution, earlier with the purified water heated and stirred, add again 1% chlorauric acid solution make the final concentration of gold chloride be ten thousand/, when treating that solution is little and boiling, add 1% sodium citrate solution fast, continue heating and stirring, stop heating after 5-10 minute, solution is cooled to room temperature, obtain the colloidal gold solution of mean diameter at 40nm-50nm, 4 ℃ of preservations are standby;
B. the adjusting of collaurum pH: the colloidal gold solution that above-mentioned steps obtains is regulated pH at 6-8;
C. colloid gold label: add albumen to be marked in the colloidal gold solution that above-mentioned steps obtains, stir after 10-30 minute, the bovine serum albumin(BSA) that adds 10ml 10% in the every 100ml solution that obtains, 8000-12000rpm is centrifugal 30 minutes behind the mixing, abandon supernatant, precipitate the treated collaurum albumen composition that obtains;
D. the preparation of collaurum pad: the collaurum albumen composition that above-mentioned steps obtains is layered on the pad uniformly, and low-temperature freeze-dry cuts into rectangular standby;
3) preparation of japonice ovum antigen:
After the japonice ovum antigen grinding, in the normal saline solution of adding 0.85%, place-20 ℃ of freeze overnight, the dissolving on daytime, so multigelation is placed on for 5-10 time in the rubble ice, uses ultrasonic disruption 3 minutes, broken 10 times repeatedly, 12000rpm high speed centrifugation 30 minutes is got supernatant and is japonice ovum antigen;
4) nitrocellulose filter is provided with detection line and nature controlling line: the anti-human IgG bag that will be drawn into the 1mg/ml japonice ovum antigen in the detection line syringe pump by machine and be drawn into 1mg/ml in the nature controlling line syringe pump with bag is formed detection line and nature controlling line respectively to nitrocellulose filter, standby after 37 ℃ of dried overnight.
6, the preparation method of quick test kit of schistosomiasis according to claim 5, it is characterized in that, described precipitation is to handle like this to obtain the collaurum albumen composition: precipitation is according to the ratio of 100ml: 10ml, and every 100ml collaurum suspends with the PBS solution that 10ml contains 1% bovine serum albumin(BSA), 0.1% polyglycol, pH=8.2.
7, the preparation method of quick test kit of schistosomiasis according to claim 6, it is characterized in that, described sample pad adopts glass fibre membrane or nonwoven fabrics as the bag suppressed by vector, and the collaurum pad adopts glass fibre membrane, nonwoven fabrics or polyester film as the bag suppressed by vector.
8, the preparation method of quick test kit of schistosomiasis according to claim 6 is characterized in that, described nitrocellulose filter adopts nylon membrane, carboxylation cellulose membrane or pure cellulose film to substitute.
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