CN105223366A - For the microtubule of Blood grouping, integral type ABO/RhD blood typing test card and preparation - Google Patents
For the microtubule of Blood grouping, integral type ABO/RhD blood typing test card and preparation Download PDFInfo
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Abstract
The invention discloses a kind of microtubule for Blood grouping, integral type ABO/RhD blood typing test card and preparation method, the microtubule of this Blood grouping up comprises gel, antibody working fluid and serum separation gel successively from bottom, containing blood group antibody and the antibody diluent making described blood group antibody maintenance activity in described antibody working fluid, the proportion of described serum separation gel is between 1.075 ~ 1.077, thixotroping can be there is, by solid fluidify under the centrifugal action of 150G ~ 300G; The proportion of described antibody diluent is greater than serum separation gel and is less than red blood cell.The integral type ABO/RhD blood typing test card adopting microtubule of the present invention to make directly can be added periphery whole blood and be detected, and do not need external all whole bloods to carry out after centrifugal taking-up red blood cell dilutes to detect again, under the sensitivity ensureing blood type test card and specific prerequisite, the operation steps of traditional ABO/RhD blood typing test card can be reduced, raise the efficiency.
Description
Technical field
The present invention relates to Blood grouping, particularly relate to a kind of microtubule for Blood grouping, integral type ABO/RhD blood typing test card and preparation method.
Background technology
Since self-discovery abo blood group, abo blood group is widely used in clinical, and the accurate sizing of abo blood group is extremely important, is basis and the guarantee of safe transfusion.Rh blood group system is the important blood group system being only second to ABO blood group system, and be also a system the most complicated in erythrocyte blood type system, the antigen in Rh blood group system comprises D, C, E etc., and wherein D antigen is the strongest.It is that RhD is positive that erythrocyte surface contains D antigen, not containing D antigen is that RhD is negative, after RhD negative patient accepts RhD positive blood, have at least 20% patient can produce immune response to D antigen, can hemolytic reaction be there is contact this antigen in second time body after, therefore RhD be shaped also very important.
Current ABO/RhD blood typing test card needs the red blood cell after adding dilution just can detect, complex operation step, inefficiency.
Summary of the invention
In order to make up above-mentioned the deficiencies in the prior art, the present invention proposes a kind of microtubule for Blood grouping, integral type ABO/RhD blood typing test card and preparation method.
Technical matters of the present invention is solved by following technical scheme:
A kind of microtubule for Blood grouping provided by the invention is: described microtubule up comprises gel, antibody working fluid and serum separation gel successively from bottom; Containing blood group antibody and the antibody diluent making described blood group antibody maintenance activity in described antibody working fluid; Between 1.075 ~ 1.077, can there is thixotroping in the proportion of described serum separation gel, by solid fluidify under the centrifugal action of 150G ~ 300G; The proportion of described antibody diluent is greater than serum separation gel and is less than red blood cell.
Integral type ABO/RhD blood typing test card provided by the invention is: described test card has 8 microtubules, described 8 microtubules respectively:
First microtubule: be described microtubule, and the blood group antibody contained in described antibody working fluid is anti-A monoclonal antibody;
Second microtubule: be described microtubule, and the blood group antibody contained in described antibody working fluid is anti-B monoclonal antibody;
3rd microtubule: be described microtubule, and the blood group antibody contained in described antibody working fluid is anti-D monoclonal antibody;
4th microtubule: up comprise gel, antibody diluent and serum separation gel successively from the bottom of described 4th microtubule;
5th microtubule ~ the 8th microtubule: repeat the first microtubule ~ the 4th microtubule respectively.
The preparation method of integral type ABO/RhD blood typing test card provided by the invention, comprises following steps:
(1) prepare antibody diluent, the proportion of described antibody diluent is greater than serum separation gel and is less than red blood cell;
(2) gel swelling: adopt physiological saline or described antibody diluent to carry out swelling to gel;
(3) antibody working fluid is prepared: respectively the described antibody diluent proportions that anti-A monoclonal antibody, anti-B monoclonal antibody and anti-D monoclonal antibody add 7 parts by volume by the antibody of every 1 parts by volume is become described antibody working fluid;
(4) prepare specificity gel: the ratio adding antibody working fluid described in 1.2-1.7ml according to swelling gel described in every 1g be mixed with respectively there is IgM character anti-monoclonal anti A gel, there is the anti-monoclonal anti B gel of IgM character and there is the anti-monoclonal anti D gel of IgM character; And preparation neutral gum: add the proportions of antibody diluent described in 1.2-1.7ml according to swelling gel described in every 1g and become neutral gum; Wherein: anti-A antibody titer >=128, anti-B antibody titer >=128, anti-D antibody titer >=128;
(5) packing: according to the filling amount of every pipe 45-55 μ l, join in the first microtubule, the second microtubule, the 3rd microtubule and the 4th microtubule respectively by described anti-monoclonal anti A gel, anti-monoclonal anti B gel, anti-monoclonal anti D gel, neutral gum, the 5th microtubule ~ the 8th microtubule repeats the first microtubule ~ the 4th microtubule respectively;
(6) prepare serum separation gel, between 1.075 ~ 1.077, can there is thixotroping in the proportion of described serum separation gel, by solid fluidify under the centrifugal action of 150G ~ 2000G;
(7) in the first microtubule ~ the 8th microtubule, adding the described serum separation gel of 0.8g-2g respectively, the centrifugal certain hour of 150G-300G, making described serum separation gel generation thixotroping by catching after solid fluidify on the interface of described antibody working fluid;
(8) sealing, labeling and encapsulation.
The beneficial effect that the present invention is compared with the prior art is: integral type ABO/RhD blood typing test card of the present invention directly can be added periphery whole blood and be detected, and do not need external all whole bloods to carry out after centrifugal taking-up red blood cell dilutes to detect again, under the sensitivity ensureing blood type test card and specific prerequisite, the operation steps of traditional ABO/RhD blood typing test card can be reduced, raise the efficiency.Concrete, the serum separation gel proportion that the present invention adopts is greater than serum and is less than red blood cell, proportion is between 1.075-1.077, thixotroping can be there is by solid fluidify under certain centrifugal force condition, the thixotropy that blood group separation gel is good is the necessary condition extending red blood cell and blood group antibody generation agglutinating reaction, the thixotropy of serum separation gel will meet red blood cell and rest on ample time in antibody working fluid, just be sunken at the bottom of pipe in order to avoid red blood cell can not react with blood group antibody, cause false-negative testing result.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the microtubule for Blood grouping in the specific embodiment of the invention.
Embodiment
Below contrast accompanying drawing and combine preferred embodiment the invention will be further described.
The invention provides a kind of microtubule for Blood grouping, in a specific embodiment, as shown in Figure 1, microtubule 1 up comprises gel 11, antibody working fluid 12 and serum separation gel 13 successively from bottom, containing blood group antibody and the antibody diluent making blood group antibody maintenance activity in antibody working fluid; Between 1.075 ~ 1.077, can there is thixotroping in the proportion of serum separation gel 13, by solid fluidify under the centrifugal action of 150G ~ 2000G; The proportion of antibody diluent is greater than serum separation gel and is less than red blood cell.
The proportion relation of each material is as follows: serum < serum separation gel < antibody specific gravity liquid < red blood cell, in a preferred embodiment:
The proportion of antibody diluent is between 1.078-1.082, and pH value is between 6.6-6.8.
Serum separation gel comprises each component of following weight portion: cyclopentadiene oligomers 35-45 part, organic gelling agent 12-25 part, organic gelling agent spreading agent 15-30 part, viscosity depressant 8-12 part and proportion correctives 8-12 part.In a more preferred embodiment, serum separation gel comprises each component of following weight portion: cyclopentadiene oligomers 40 parts, organic gelling agent 20 parts, organic gelling agent spreading agent 20 parts, viscosity depressant 10 parts and proportion correctives 10 parts.
Gel is sephadex, and particle diameter is 40-60 μm.
The centrifugal force of serum separation gel generation thixotroping is 200G, and centrifugation time is 2min.
Under same time (this experiment employing 2 minutes), design centrifugal force gradient 80G, 100G, 120G, 150G, 180G, 200G, 250G, 300G, 400G, experiment proves, under the centrifugal force being greater than 150G, serum separation gel well can be separated red blood cell and serum, lower than then effect is poor; And if centrifugal force is higher than 300G, although red blood cell and serum can be separated by serum separation gel, because centrifugal force is excessive, red blood cell does not have the blood group antibody in time enough and antibody working fluid to react just to sink at the bottom of pipe, be unfavorable for correctly differentiating blood group.
Under identical centrifugal force, carry out three groups of experiments, be 150G, 180G and 200G respectively, designs a time gradient 30s, 1min, 1.5min, 2min, and experiment proves, when 200G, centrifugal 2 minutes effects are better.
Described organic gelling agent is dibenzylidene sorbitol; Described organic gelling agent spreading agent is at least one in polyox-yethylene-polyoxypropylene block copolymer, 1-Methyl-2-Pyrrolidone, DMSO, DMA and DMF; Described viscosity depressant is at least one in phthalic acid two (2-ethylhexyl) ester and epoxy resin; Described proportion correctives is silicon dioxide, titania, the chlorinated paraffin of chlorination degree 40%, the chlorinated paraffin of chlorination degree 70% of particle diameter 5 μm-10 μm.
The ratio of the volume of described antibody working fluid and the weight of described gel is between 1:3 to 1:5; The addition of described serum separation gel is 0.8-2g, more optimizedly adds 1g.The present invention also provides a kind of integral type ABO/RhD blood typing test card, in a specific embodiment, test card has 8 microtubules, 8 microtubules respectively:
First microtubule: be the microtubule in above-mentioned embodiment, and the blood group antibody contained in antibody working fluid is anti-A monoclonal antibody;
Second microtubule: be the microtubule in above-mentioned embodiment, and the blood group antibody contained in described antibody working fluid is anti-B monoclonal antibody;
3rd microtubule: be the microtubule in above-mentioned embodiment, and the blood group antibody contained in described antibody working fluid is anti-D monoclonal antibody;
4th microtubule: up comprise gel, antibody diluent and serum separation gel successively from the bottom of the 4th microtubule is also not containing blood group antibody in the 4th microtubule;
5th microtubule ~ the 8th microtubule: repeat the first microtubule ~ the 4th microtubule respectively.
The present invention also provides a kind of preparation method of integral type ABO/RhD blood typing test card, in a specific embodiment, comprises following steps:
(1) prepare antibody diluent, the proportion of described antibody diluent is greater than serum separation gel and is less than red blood cell;
(2) gel swelling: adopt physiological saline or described antibody diluent to carry out swelling to gel;
(3) antibody working fluid is prepared: respectively the described antibody diluent proportions that anti-A monoclonal antibody, anti-B monoclonal antibody and anti-D monoclonal antibody add 7 parts by volume by the antibody of every 1 parts by volume is become described antibody working fluid;
(4) prepare specificity gel: the ratio adding antibody working fluid described in 1.2-1.7ml according to swelling gel described in every 1g be mixed with respectively there is IgM character anti-monoclonal anti A gel, there is the anti-monoclonal anti B gel of IgM character and there is the anti-monoclonal anti D gel of IgM character; And preparation neutral gum: add the proportions of antibody diluent described in 1.2-1.7ml according to swelling gel described in every 1g and become neutral gum; Wherein: anti-A antibody titer >=128, anti-B antibody titer >=128, anti-D antibody titer >=128;
(5) packing: according to the filling amount of every pipe 45-55 μ l, join in the first microtubule, the second microtubule, the 3rd microtubule and the 4th microtubule respectively by described anti-monoclonal anti A gel, anti-monoclonal anti B gel, anti-monoclonal anti D gel, neutral gum, the 5th microtubule ~ the 8th microtubule repeats the first microtubule ~ the 4th microtubule respectively;
(6) prepare serum separation gel, between 1.075 ~ 1.077, can there is thixotroping in the proportion of described serum separation gel, by solid fluidify under the centrifugal action of 150G ~ 2000G;
(7) in the first microtubule ~ the 8th microtubule, adding the described serum separation gel of 0.8g-2g respectively, the centrifugal certain hour of 150G-300G, making described serum separation gel generation thixotroping by catching after solid fluidify on the interface of described antibody working fluid;
(8) sealing, labeling and encapsulation.
In a preferred embodiment:
Described step (6) specifically comprises the steps:
(61) by 35-45 part cyclopentadiene oligomers heating and melting;
(62) in the cyclopentadiene oligomers melted, organic gelling agent 12-25 part is added, organic gelling agent spreading agent 15-30 part and viscosity depressant 8-12 part, kneaded mixture 20-40 minute;
(63) be cooled to normal temperature (i.e. 20-30 DEG C), add proportion correctives 8-12 part, vacuum kneading potpourri 20-40 minute under the vacuum tightness of 0.1bar-0.5bar.
The process of the described antibody diluent of preparation in described step (1) is as follows: in every 1L pure water, add 1.2-1.7g sodium chloride, 12-30g glycocoll, 0.2-0.3g sodium hydrogen phosphate, 0.46-0.56g sodium dihydrogen phosphate, 10-20g bovine serum albumin(BSA), 12-25g glycerine, 0.45-0.68g methyl p-hydroxybenzoate and 0.1-0.16g propylparaben.
In a preferred embodiment, the preparation method of this integral type ABO/RhD blood typing test card comprises following steps:
(1) antibody diluent is prepared, the proportion of described antibody diluent is greater than serum separation gel and is less than red blood cell, and in this example, antibody diluent proportion is 1.078-1.082, pH is 6.6, fill a prescription as follows: in 1L pure water, add 1.5g sodium chloride, 20g glycocoll, 0.26g sodium hydrogen phosphate, 0.52g sodium dihydrogen phosphate, bovine serum albumin(BSA) 10g, glycerine 20g, methyl p-hydroxybenzoate 0.6g and propylparaben 0.13g.
(2) gel swelling, directly adopts antibody diluent to carry out swelling to gel, specifically comprises the steps: in this example
(21) in every 1g gel (adopting sephadex in this example), add 2-10ml antibody diluent (for 1g gel adds 2ml antibody diluent in this example), suspended gel, carries out moist heat sterilization, for subsequent use after sterilizing.
(22) gel after step (21) sterilizing is placed certain hour (being 6 hours in this example), make gel natural subsidence, remove supernatant, the antibody diluent adding volume ratio 1:1.2 (gel of sedimentation and the ratio of antibody diluent) is again resuspended, repetition like this twice, the damaged fragment of removing gel, the swelling gel of collecting granules size even (in this example, particle diameter is within the scope of 40-60 μm).
(3) antibody working fluid is prepared: respectively by anti-A monoclonal antibody, anti-B monoclonal antibody and anti-D monoclonal antibody (in this example, three kinds of antibody are all purchased from Merck Millipore Corp.) add the antibody dilution liquid proportional of 7 parts by volume (in this example by the antibody of every 1 parts by volume, concrete, the volume of each antibody is 5ml, and the volume for the antibody diluent diluting each antibody is respectively 35ml) be mixed with antibody working fluid.
(4) prepare specificity gel: the ratio (in this example be 1g swell gel add the antibody diluent of 1.5ml) adding 1.2-1.7ml antibody working fluid according to the gel that every 1g is swelling be mixed with respectively there is IgM character anti-monoclonal anti A gel, there is the anti-monoclonal anti B gel of IgM character and there is the anti-monoclonal anti D gel of IgM character; And preparation neutral gum: the ratio (in this example be 1g swell gel add the antibody diluent of 1.5ml) adding 1.2-1.7ml antibody diluent according to the gel that every 1g is swelling is mixed with neutral gum; Wherein: anti-A antibody titer >=128, anti-B antibody titer >=128, anti-D antibody titer >=128.
(5) packing: according to the filling amount of every pipe 50 μ l, join in the first microtubule, the second microtubule, the 3rd microtubule and the 4th microtubule respectively by described anti-monoclonal anti A gel, anti-monoclonal anti B gel, anti-monoclonal anti D gel, neutral gum, the 5th microtubule ~ the 8th microtubule repeats the first microtubule ~ the 4th microtubule respectively.
(6) serum separation gel is prepared, specific as follows:
(61) by 40 parts of cyclopentadiene oligomers heating and melting at 130 DEG C.
(62) in the cyclopentadiene oligomers melted, add organic gelling agent (being dibenzylidene sorbitol in this example) 20 parts, organic gelling agent spreading agent (being polyox-yethylene-polyoxypropylene block copolymer in this example) 20 parts and viscosity depressant (being phthalic acid two (2-ethylhexyl) ester in this example) 10 parts, kneaded mixture 30 minutes.
(63) be cooled to normal temperature, add proportion correctives (adopting titania in this example) 10 parts, vacuum kneading potpourri 30 minutes.
(7) in the first microtubule ~ the 8th microtubule, adding 1g serum separation gel respectively, centrifugal 3 minutes of 200G, making serum separation gel generation thixotroping by catching after solid fluidify on the interface of antibody working fluid.
(8) sealing, labeling and encapsulation, wherein, sealing refers to: a point mouth of pipe aluminium foil for the microtubule installed is carried out heating power plastic packaging; Labeling refers to: after sealing completes, labeling on each microtubule, forms integral type ABO/RhD blood typing test card; Encapsulation refers to: carry out aseptic plastic packaging and mounted box to integral type ABO/RhD blood typing test card.
Below design two groups of experiments respectively, adopt the integral type ABO/RhD blood typing test card (hereinafter referred to as integrated card) of above embodiment to carry out Blood grouping with the abo blood group test card (hereinafter referred to as routine card) of Changchun Bo Xun company and contrast as follows:
First group of experiment:
1, extract unknown blood group blood donor 10 people, everyone extracts peripheral blood 10ml (sample 1 ~ sample 10).
2, getting above-mentioned peripheral blood 2.5ml adds in the test tube containing set accelerator, centrifugal 2 minutes of 300G, gets 16 μ l packed red cellses, adds 1984 μ l physiological saline, is mixed with 0.8% red cell suspension.
3, get integrated card and conventional each 50 of card, centrifugal 2 minutes of 200G, tears aluminium film.
4, in integrated card, directly add 2 μ l peripheral bloods, in conventional card, add the red cell suspension of 50 μ l simultaneously.
5, the routine card having added red cell suspension is put into hydro-extractor 70G centrifugal 1 minute, then centrifugal 4 minutes of 100G.
6, the integrated card adding peripheral blood is put into hydro-extractor 200G centrifugal 2 minutes, then centrifugal 4 minutes of 100G.
7, card taking result of determination, result is as following table one.
Table one unknown blood group pattern detection result
| Sample | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Integrated card | A | O | B | A | B | B | AB | B | O | A |
| Conventional card | A | O | B | A | B | B | AB | B | O | A |
Remarks: the RhD testing result of above-mentioned 10 samples is feminine gender.
Second group of experiment:
1, prepare A, B, O and AB blood group peripheral blood 10 parts, wherein 5 parts is that RhD is negative, and other 5 parts is that RhD is positive, every part of 10ml.
2, getting above-mentioned peripheral blood 2.5ml adds in the test tube containing set accelerator, centrifugal 2 minutes of 300G, gets 16 μ l packed red cellses, adds 1984 μ l physiological saline, is mixed with 0.8% red cell suspension.
3, get integrated card and conventional each 50 of card, centrifugal 2 minutes of 200G, tears aluminium film.
4, in integrated card, directly add 2 μ l peripheral bloods, in conventional card, add the red cell suspension of 50 μ l simultaneously.
5, the routine card having added red cell suspension is put into hydro-extractor 70G centrifugal 1 minute, then centrifugal 4 minutes of 100G.
6, the integrated card adding peripheral blood is put into hydro-extractor 200G centrifugal 2 minutes, then centrifugal 4 minutes of 100G.
7, card taking result of determination, result is as following table two.
Table two known blood group pattern detection result
From the above experimental results, under the accuracy prerequisite ensureing blood type test card, the integral type ABO/RhD blood typing test card of the present embodiment directly can be added periphery whole blood and be detected, and do not need external all whole bloods to carry out after centrifugal taking-up red blood cell dilutes to detect again, the operation steps of traditional ABO/RhD blood typing test card can be reduced, raise the efficiency.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For those skilled in the art, without departing from the inventive concept of the premise, some equivalent to substitute or obvious modification can also be made, and performance or purposes identical, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. for a microtubule for Blood grouping, it is characterized in that: described microtubule up comprises gel, antibody working fluid and serum separation gel successively from bottom; Containing blood group antibody and the antibody diluent making described blood group antibody maintenance activity in described antibody working fluid; Between 1.075 ~ 1.077, can there is thixotroping in the proportion of described serum separation gel, by solid fluidify under the centrifugal action of 150G ~ 300G; The proportion of described antibody diluent is greater than serum separation gel and is less than red blood cell.
2. microtubule as claimed in claim 1, is characterized in that: the proportion of described antibody diluent is between 1.078-1.082, and pH value is between 6.6-6.8.
3. microtubule as claimed in claim 1, it is characterized in that: described serum separation gel comprises each component of following weight portion: cyclopentadiene oligomers 35-45 part, organic gelling agent 12-25 part, organic gelling agent spreading agent 15-30 part, viscosity depressant 8-12 part and proportion correctives 8-12 part.
4. microtubule as claimed in claim 1, it is characterized in that: described gel is sephadex, particle diameter is 40-60 μm.
5. microtubule as claimed in claim 3, is characterized in that: described organic gelling agent is dibenzylidene sorbitol; Described organic gelling agent spreading agent is at least one in polyox-yethylene-polyoxypropylene block copolymer, 1-Methyl-2-Pyrrolidone, DMSO, DMA and DMF; Described viscosity depressant is at least one in phthalic acid two (2-ethylhexyl) ester and epoxy resin; Described proportion correctives is silicon dioxide, titania, the chlorinated paraffin of chlorination degree 40%, the chlorinated paraffin of chlorination degree 70% of particle diameter 5 μm-10 μm.
6. microtubule as claimed in claim 1, it is characterized in that: in described microtubule, the ratio of the volume of described antibody working fluid and the weight of described gel is between 1:3 to 1:5; The addition of described serum separation gel is 0.8g-2g.
7. an integral type ABO/RhD blood typing test card, is characterized in that: described test card has 8 microtubules, described 8 microtubules respectively:
First microtubule: be the microtubule described in claim 1-6 any one, and the blood group antibody contained in described antibody working fluid is anti-A monoclonal antibody;
Second microtubule: be the microtubule described in claim 1-6 any one, and the blood group antibody contained in described antibody working fluid is anti-B monoclonal antibody;
3rd microtubule: be the microtubule described in claim 1-6 any one, and the blood group antibody contained in described antibody working fluid is anti-D monoclonal antibody;
4th microtubule: up comprise gel, antibody diluent and serum separation gel successively from the bottom of described 4th microtubule;
5th microtubule ~ the 8th microtubule: repeat the first microtubule ~ the 4th microtubule respectively.
8. a preparation method for integral type ABO/RhD blood typing test card, is characterized in that, comprises following steps:
(1) prepare antibody diluent, the proportion of described antibody diluent is greater than serum separation gel and is less than red blood cell;
(2) gel swelling: adopt physiological saline or described antibody diluent to carry out swelling to gel;
(3) antibody working fluid is prepared: respectively the described antibody diluent proportions that anti-A monoclonal antibody, anti-B monoclonal antibody and anti-D monoclonal antibody add 7 parts by volume by the antibody of every 1 parts by volume is become described antibody working fluid;
(4) prepare specificity gel: the ratio adding antibody working fluid described in 1.2-1.7ml according to swelling gel described in every 1g be mixed with respectively there is IgM character anti-monoclonal anti A gel, there is the anti-monoclonal anti B gel of IgM character and there is the anti-monoclonal anti D gel of IgM character; And preparation neutral gum: add the proportions of antibody diluent described in 1.2-1.7ml according to swelling gel described in every 1g and become neutral gum; Wherein: anti-A antibody titer >=128, anti-B antibody titer >=128, anti-D antibody titer >=128;
(5) packing: according to the filling amount of every pipe 45-55 μ l, join in the first microtubule, the second microtubule, the 3rd microtubule and the 4th microtubule respectively by described anti-monoclonal anti A gel, anti-monoclonal anti B gel, anti-monoclonal anti D gel, neutral gum, the 5th microtubule ~ the 8th microtubule repeats the first microtubule ~ the 4th microtubule respectively;
(6) prepare serum separation gel, between 1.075 ~ 1.077, can there is thixotroping in the proportion of described serum separation gel, by solid fluidify under the centrifugal action of 150G ~ 2000G;
(7) in the first microtubule ~ the 8th microtubule, adding the described serum separation gel of 0.8g-2g respectively, the centrifugal certain hour of 150G-300G, making described serum separation gel generation thixotroping by catching after solid fluidify on the interface of described antibody working fluid;
(8) sealing, labeling and encapsulation.
9. preparation method as claimed in claim 8, it is characterized in that, described step (6) specifically comprises the steps:
(61) by 35-45 part cyclopentadiene oligomers heating and melting;
(62) in the cyclopentadiene oligomers melted, organic gelling agent 12-25 part is added, organic gelling agent spreading agent 15-30 part and viscosity depressant 8-12 part, kneaded mixture 20-40 minute;
(63) be cooled to normal temperature, add proportion correctives 8-12 part, vacuum kneading potpourri 20-40 minute under the vacuum tightness of 0.1bar-0.5bar.
10. preparation method as claimed in claim 8, it is characterized in that, the process of the described antibody diluent of preparation in described step (1) is as follows: in every 1L pure water, add 1.2-1.7g sodium chloride, 12-30g glycocoll, 0.2-0.3g sodium hydrogen phosphate, 0.46-0.56g sodium dihydrogen phosphate, 10-20g bovine serum albumin(BSA), 12-25g glycerine, 0.45-0.68g methyl p-hydroxybenzoate and 0.1-0.16g propylparaben.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510601037.4A CN105223366B (en) | 2015-09-18 | 2015-09-18 | Micropipe for blood grouping, one-piece ABO/RhD blood typing test card for blood grouping and preparation thereof |
| HK16103536.7A HK1215813A1 (en) | 2015-09-18 | 2016-03-25 | Microtube for detecting blood groups, an integrated abo/rhd blood groups detection card and its preparation abo/rhd |
| PCT/CN2016/078941 WO2017045397A1 (en) | 2015-09-18 | 2016-04-11 | Micropipe for blood grouping, one-piece abo/rhd blood typing test card, and preparation therefor |
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|---|---|---|---|
| CN201510601037.4A CN105223366B (en) | 2015-09-18 | 2015-09-18 | Micropipe for blood grouping, one-piece ABO/RhD blood typing test card for blood grouping and preparation thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586251A (en) * | 2016-03-18 | 2016-05-18 | 深圳市达科为生物工程有限公司 | Lymphocyte separation tube, manufacturing method thereof and lymphocyte separation method |
| WO2017045397A1 (en) * | 2015-09-18 | 2017-03-23 | 深圳市达科为生物工程有限公司 | Micropipe for blood grouping, one-piece abo/rhd blood typing test card, and preparation therefor |
| CN108845139A (en) * | 2018-05-04 | 2018-11-20 | 江苏中济万泰生物医药有限公司 | Antibody sample dilution saves liquid and preparation method thereof |
| CN109991408A (en) * | 2019-04-10 | 2019-07-09 | 福州迈新生物技术开发有限公司 | A kind of antibody diluent composition, antibody diluent and preparation method thereof |
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| CN111337693A (en) * | 2020-03-30 | 2020-06-26 | 江苏中济万泰生物医药有限公司 | Preparation method of Rh blood type detection card |
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| CN113189354B (en) * | 2021-04-30 | 2023-09-19 | 重庆国际旅行卫生保健中心(重庆海关口岸门诊部) | Portable quick blood typing detection assembly |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017045397A1 (en) * | 2015-09-18 | 2017-03-23 | 深圳市达科为生物工程有限公司 | Micropipe for blood grouping, one-piece abo/rhd blood typing test card, and preparation therefor |
| CN105586251A (en) * | 2016-03-18 | 2016-05-18 | 深圳市达科为生物工程有限公司 | Lymphocyte separation tube, manufacturing method thereof and lymphocyte separation method |
| CN105586251B (en) * | 2016-03-18 | 2018-02-27 | 深圳市达科为生物工程有限公司 | A kind of separation of lymphocytes pipe and preparation method thereof, separation of lymphocytes method |
| CN108845139A (en) * | 2018-05-04 | 2018-11-20 | 江苏中济万泰生物医药有限公司 | Antibody sample dilution saves liquid and preparation method thereof |
| CN109991408A (en) * | 2019-04-10 | 2019-07-09 | 福州迈新生物技术开发有限公司 | A kind of antibody diluent composition, antibody diluent and preparation method thereof |
| CN112577796A (en) * | 2020-11-02 | 2021-03-30 | 广东省中医院（广州中医药大学第二附属医院、广州中医药大学第二临床医学院、广东省中医药科学院） | Erythrocyte heat diffusion elution kit and application thereof in ABO blood type identification |
| CN112684190A (en) * | 2020-12-04 | 2021-04-20 | 上海润普生物技术有限公司 | ABO blood type positive typing and RhD blood type detection card and preparation method thereof |
| CN113219183A (en) * | 2021-06-16 | 2021-08-06 | 尚建华 | Liquid glue blood type detection card, preparation method and blood type detection system |
| CN113219183B (en) * | 2021-06-16 | 2024-04-19 | 尚建华 | Liquid rubber blood type detection card, preparation method and blood type detection system |
Also Published As
| Publication number | Publication date |
|---|---|
| HK1215813A1 (en) | 2016-09-15 |
| WO2017045397A1 (en) | 2017-03-23 |
| CN105223366B (en) | 2017-01-18 |
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