CN210108911U

CN210108911U - Single-person vacuum flow cytometry absolute counting kit

Info

Publication number: CN210108911U
Application number: CN201920799927.4U
Authority: CN
Inventors: 王敏
Original assignee: Bard Biotechnology Co Ltd
Current assignee: Bard Biotechnology Co Ltd
Priority date: 2019-05-27
Filing date: 2019-05-27
Publication date: 2020-02-21
Anticipated expiration: 2029-05-27

Abstract

The utility model discloses a single share's absolute count kit of flow cytometry in vacuum belongs to kit technical field, including reagent pipe, sealed lid and freeze-drying granule, sealed lid fixed connection is in the upper end of reagent pipe, and the freeze-drying granule is installed in the inner bottom end of reagent pipe, and sealed lid comprises the sealed lid of stereoplasm annular and soft sealed lid, and soft sealed lid is located the middle part position of sealed lid, and the sealed lid fixed connection of stereoplasm annular is in the outer end of soft sealed lid, contains the reference cell of detect reagent and absolute count, can be used to detect u1 blood specimen.

Description

Single-person vacuum flow cytometry absolute counting kit

Technical Field

The utility model relates to a kit technical field, more specifically say, relate to a single absolute count kit of flow cytometry in vacuum.

Background

The lymphocyte level is an important index of the cellular immunity and humoral immunity functions of an organism, and when the quantity and the functions of different lymphocyte subsets are abnormally changed, the organism can generate a series of pathological changes and immune dysfunction, so that the detection of the change of the lymphocyte subsets of human beings has very important significance for early discovery and control of some diseases, guidance of clinical treatment and assessment of the immune state of the organism.

Flow cytometry is the current standard for clinical detection of lymphoid subpopulations, using this method allows simultaneous detection of surface antigens of concentrated lymphocytes, allows the relative proportion of individual subpopulations to be obtained, from which the absolute number of subpopulation cells can be calculated by means of a reference.

The reagent for the current flow cytometry detection is usually 50-100 persons per bottle, the reagent and the blood sample are required to be respectively detected and then mixed and reacted in a new flow tube, and the blood collection amount is usually 1ml, so that the absolute count of lymphocytes by the flow cytometry is limited for people who are difficult to take the venous blood collection, such as children.

SUMMERY OF THE UTILITY MODEL

1. Technical problem to be solved

To the problem that exists among the prior art, the utility model aims at providing a flow cytometry absolute counting kit of vacuum singleness, it contains the reference cell of detect reagent and absolute count, can be used to detect 25ul blood samples.

2. Technical scheme

In order to solve the above problem, the utility model adopts the following technical scheme:

the utility model provides a single one's absolute count kit of flow cytometry in vacuum, includes reagent pipe, sealed lid and freeze-drying granule, sealed lid fixed connection is in the upper end of reagent pipe, the freeze-drying granule is installed in the interior bottom of reagent pipe, sealed lid comprises sealed lid of stereoplasm annular and soft sealed lid, soft sealed lid is located the middle part position of sealed lid, the sealed lid fixed connection of stereoplasm annular contains the reference cell of detect reagent and absolute count in the outer end of soft sealed lid, can be used to detect ul blood sample.

Furthermore, the reagent tube is a flow tube, and can be directly used on a flow cytometer to obtain a sample.

Furthermore, the soft sealing cover is soft silica gel, can be punctured by a needle head, and can easily penetrate through the capillary glass tube.

Further, the lyophilized particles are all the reagents and reference cells required.

Further, the freeze-dried particles are spherical or ellipsoidal.

3. Advantageous effects

Compared with the prior art, the utility model has the advantages of:

(1) errors introduced in the measuring process are avoided.

(2) The collected trace blood is used directly, and the required sample size is far less than ml.

(3) The spherical or ellipsoidal freeze-dried particles are clearly visible in the transparent tube, and if moisture absorption or the like is not suitable for use, they can be easily found from the appearance.

Drawings

Fig. 1 is a perspective view of the present invention;

FIG. 2 is a schematic structural view of the present invention;

fig. 3 is a usage state diagram of the present invention.

The reference numbers in the figures illustrate:

1 reagent tube, 2 sealing cover, 3 freeze-drying particles.

Detailed Description

The technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiment of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention based on the embodiments of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "provided", "sleeved/connected", "connected", and the like are to be understood in a broad sense, such as "connected", which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example (b):

referring to fig. 1-2, a vacuum single-person absolute counting kit for flow cytometry comprises a reagent tube 1, a sealing cover 2 and freeze-dried particles 3, wherein the reagent tube 1 is a flow tube, the reagent tube 1 can be directly used on a flow cytometer to obtain a sample, the reagent tube 1 is transparent to facilitate observation of the conditions in the reagent tube 1, the sealing cover 2 is fixedly connected to the upper end of the reagent tube 1, the sealing cover 2 has excellent sealing performance, the sealing cover 2 comprises a hard annular sealing cover and a soft sealing cover, the soft sealing cover is positioned in the middle of the sealing cover 2, the soft sealing cover is made of soft silica gel, the soft silica gel can be pierced by a needle and easily penetrates through a capillary glass tube, the hard annular sealing cover is fixedly connected to the outer end of the soft sealing cover, the freeze-dried particles 3 are mounted at the inner bottom end of the reagent tube 1, and the freeze-dried particles 3 are all required reagents and reference, the freeze-dried particles 3 are spherical or ellipsoidal, and the spherical or ellipsoidal freeze-dried particles 3 are clearly visible in the transparent tube, and can be easily found from the appearance if moisture absorption or the like is not suitable for use.

The working principle is as follows: referring to fig. 3, after taking a trace amount of blood from a glass capillary, a soft sealing cover at the middle position of a sealing cover 2 is firstly pierced by a syringe needle, and then the glass capillary is quickly inserted into the sealing cover, because of relative vacuum in a reagent tube 1, blood in the capillary can be sucked out and dripped to the bottom of the tube to be melted and reacted with freeze-dried particles 3, and no reagent or blood sample needs to be taken in the process, so that the advantages are three points: errors introduced in the measuring process are avoided; directly using the collected trace blood, wherein the required sample size is far less than 1 ml; the spherical or ellipsoidal freeze-dried particles 3 are clearly visible in the transparent tube, and if moisture absorption or the like is not suitable for use, they can be easily found from the appearance.

The above description is only the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can substitute or change the technical solution and the improvement concept of the present invention within the technical scope disclosed in the present invention.

Claims

1. The utility model provides a single count kit of flowing cytometry absolutely of portion in vacuum, includes reagent pipe (1), sealed lid (2) and freeze-drying granule (3), its characterized in that: sealed lid (2) fixed connection is in the upper end of reagent pipe (1), freeze-drying granule (3) are installed in the inner bottom end of reagent pipe (1), sealed lid (2) comprise sealed lid of stereoplasm annular and soft sealed lid, soft sealed lid is located the middle part position of sealed lid (2), the sealed outer end of lid fixed connection in soft sealed lid of stereoplasm annular.

2. A vacuum single-serving absolute flow cytometry kit as described in claim 1 wherein: the reagent tube (1) is a flow tube.

3. A vacuum single-serving absolute flow cytometry kit as described in claim 1 wherein: the soft sealing cover is made of soft silica gel.

4. A vacuum single-serving absolute flow cytometry kit as described in claim 1 wherein: the lyophilized particles (3) are all the reagents and reference cells required.

5. A vacuum single-serving absolute flow cytometry kit as described in claim 1 wherein: the freeze-dried particles (3) are spherical or ellipsoidal.