CN211426204U

CN211426204U - Blood cell analyzer

Info

Publication number: CN211426204U
Application number: CN201921382950.XU
Authority: CN
Inventors: 张兆麟; 张金泉
Original assignee: Suzhou Zhongjia Kangmei Technology Co ltd
Current assignee: Suzhou kangjianyun Medical Technology Co.,Ltd.
Priority date: 2019-06-21
Filing date: 2019-08-23
Publication date: 2020-09-04
Anticipated expiration: 2029-08-23
Also published as: CN112113895A

Abstract

The application discloses a blood cell analyzer. The blood cell analyzer comprises a sample processing plate, an amplifying counting plate and a blood cell detector; the sample processing plate is used for containing a blood cell sample; the magnifying counting plate comprises a lens; the blood cell detector includes an image sensor; the sample processing plate is detachably mounted on the amplification counting plate, and the amplification counting plate is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample amplified by the lens is transmitted to the image sensor. The application provides a hematology analyzer is disposable magnifying glass, accords with clinical examination standard, has guaranteed the reliability of result to this analyzer is with low costs, can use in daily environment.

Description

Blood cell analyzer

Technical Field

The application relates to a blood cell analyzer, which belongs to the technical field of medical instruments.

Background

In the field of modern clinical test medicine, the blood routine test is one of the most common, important and fundamental test items for disease diagnosis and health examination. The data of the size, the number, the form, the proportion and the like of various cells in the blood of the human body can be known through the examination of the item, so that a clinician can diagnose and treat diseases and the health index of physical examination crowd is provided.

At present, the routine blood examination mainly comprises two ways of manual examination and instrument examination.

Manual inspection mode: quantitatively sucking a blood sample, diluting the blood sample by a diluent or a hemolytic agent, injecting the diluted blood sample into an improved Neubauer's blood cell counting plate, counting various blood cell quantities according to different requirements under a microscope, and then calculating the quantity of various cells in a certain volume. Meanwhile, the same blood sample is pushed into a blood membrane on a glass slide, and then fixed and dyed, and leukocytes are classified according to different forms under a biological microscope. The method has the advantages that counting and classification are carried out through naked eyes of people, and the result is reliable and accurate. The disadvantages are that the speed is slow, the efficiency is low, the operation is complicated, the existing biological microscope needs to be reused, professionals are required to repeatedly adjust the objective lens, the visual field, the focal length and the like, and the accuracy of an analysis result can be influenced when different people use the microscope. But the method still remains to be used for the repeated detection when the result of the instrumental method is abnormal, or is used under the condition of special requirements of some special cases and in the places without conditional instrument purchase.

The instrument inspection mode is as follows: the blood cell analyzer can be roughly divided into a semi-automatic analyzer, a full-automatic analyzer, a white blood cell secondary classification analyzer, a white blood cell tertiary classification analyzer and a white blood cell five classification analyzer according to the complexity of the instrument, and the last two types of instruments are most commonly used at present. Firstly, the instrument can automatically or semi-automatically suck anticoagulated blood samples, then automatically distribute the samples to different containers for dilution, hemolysis and dyeing, respectively measure the resistance pulse number and the pulse size of red blood cells and platelets by the Coulter principle through red blood cells and platelet channels, and count the number of the red blood cells and the platelets. The number of the resistance pulses, the number of the pulse sizes and the volume of the white blood cells are measured by the coulter principle through the white blood cell channel, and the number of the white blood cells is counted. Meanwhile, the leucocytes are classified and counted by using the technologies of laser, visible light, cell staining and the like. The method has the advantages of simple operation, high determination speed, high automation degree and high accuracy of the counting result because the number of the counted cells is greatly more than that of a manual method. The defects are that the instrument is precise and expensive, the requirement on the operating environment is high, the daily maintenance is frequent, and the accuracy of cell morphology recognition is lower than that of a manual method.

SUMMERY OF THE UTILITY MODEL

According to the application, a hematology analyzer is provided, the magnifying counting plate in the hematology analyzer has two structures, the biconvex spherical lens of the first magnifying counting plate is installed in the tapered through hole of the counting magnifying plate, the lens of the second magnifying counting plate is an integral plano-convex lens fixed on the outer bottom surface of the main body plate in the magnifying counting plate, the biconvex spherical lens is a disposable microscope magnifier, the parameters such as field of vision, focal length and magnification are set, the clinical examination standard is met, the reliability of the result is guaranteed, and the nonprofessionals can use to carry out high-efficiency detection and analysis, and the analyzer is low in cost and can be used in daily environment.

A blood cell analyzer includes a sample processing plate, an amplifying and counting plate, and a blood cell detector;

the sample processing plate is used for containing a blood cell sample;

the magnifying counting plate comprises a lens;

the blood cell detector includes an image sensor;

the sample processing plate is detachably mounted on the amplification counting plate, and the amplification counting plate is detachably mounted in the blood cell detector, so that the optical image of the blood cell sample amplified by the lens is transmitted to the image sensor.

Optionally, the sample processing plate comprises a slide, a support, and a cover slip;

a concave sample pool is arranged on the glass slide;

the cover glass and the supporting piece are both positioned in the sample pool, and a gap for containing the blood cell sample is formed between the cover glass and the bottom surface of the sample pool through the supporting piece.

Optionally, the height of the gap in the vertical direction is 0.05-0.12 mm.

Optionally, the magnifying counting plate further comprises a main body plate;

a groove for fixing the sample processing plate is arranged on the main body plate;

the lens is fixed below the groove through the main body plate.

Optionally, the main body plate is provided with a tapered through hole communicated with the groove along the vertical direction, the lens is a biconvex spherical lens, the biconvex spherical lens is fixed in the through hole, and the through hole is gradually reduced in diameter along the vertical direction.

Optionally, the diameter of the large-diameter end of the through hole is 3-8 mm; the diameter of the small-diameter end of the through hole is 0.3-0.8 mm; the height of the through hole along the axial direction is 1-5 mm.

Preferably, the diameter range of the biconvex spherical lens is 0.5-6 mm.

Optionally, the lens is a plano-convex lens, a planar end of the plano-convex lens is fixed on the outer bottom surface of the main body plate, and a convex end of the plano-convex lens is close to the cell detector.

Optionally, the thickness of the plano-convex lens in the vertical direction is 0.05-2mm, and the diameter range of the plano-convex lens is 0.3-6 mm; the thickness of the main body plate is 1-5 mm.

Optionally, the blood cell detector further comprises a housing, an upper cover plate and a wireless transmitter;

a concave sample table for placing the amplifying and counting plate is arranged on the upper surface of the shell;

the sample table is provided with a light hole matched with the lens in the magnifying counting plate;

the image sensor and the wireless transmitter are positioned in a cavity formed by the enclosure of the shell, and the image sensor is electrically connected with the wireless transmitter;

the image sensor is matched with the light hole;

the upper cover plate is located the upper surface of casing, the orientation of upper cover plate the light source is installed to the one side of casing.

Optionally, the hemocyte analyzer further comprises a blood collector, wherein the blood collector comprises a tube body, a plug, an automatic quantitative blood collection tube, a sample adding tube and a sealing sleeve;

one end of the tube body along the axial direction is opened, and the sealing plug is detachably arranged at the opening end of the tube body;

the automatic quantitative blood collection tube and the sample adding tube are respectively fixed on two sides of the sealing plug along the axial direction, and the automatic quantitative blood collection tube is positioned in the tube body;

the automatic quantitative blood collection tube is communicated with the sample adding tube;

the seal cover is installed at one end of the sample adding pipe.

Preferably, the blood cell analyzer further comprises a data analyzer comprising a data receiver, a data processor and a display screen;

the data receiver receives a data signal in the wireless transmitter;

the data receiver is electrically connected with the data processor;

the data processor is electrically connected with the display screen.

In the present application, the "diameter of the large-diameter end of the through-hole" refers to the maximum value of the inner diameter of the tapered through-hole;

the "diameter of the small-diameter end of the through-hole" means the minimum value of the inner diameter of the tapered through-hole.

The beneficial effects that this application can produce include:

(1) the application provides a blood cell analyzer is a wireless hand-held type intelligence blood cell analyzer. The hematocytoscopy is the gold standard of blood clinical examination, the lens in the central area of the groove of the counting magnifying plate in the application is a micron-sized multi-specification optical magnifying lens, the magnification is different from 50 times to 500 times, the magnification is determined by the diameter and the focal length of the lens, the field of view, the focal length, the magnification and the resolution ratio are set, the magnifying lens can replace a biological microscope and is a disposable magnifying lens, the clinical examination standard is met, and the reliability of results is guaranteed. This approach is not used in human and instrumental blood cell detection methods.

(2) The blood cell detector is cordless, wireless and handheld in palm, and flexible mobile terminal installation software is used for analyzing results, so that the exquisite and small blood cell detector can be used together with mobile terminals such as mobile phones and the like in the structural design of the whole product, can be placed in hands or pockets without being limited by a power supply, electromagnetic interference and temperature and humidity, and achieves the purpose of mobile internet diagnosis.

(3) The intelligent operation can be used by non-professionals, and the system has the characteristics of portability, instantaneity, reliability, internet and the like, so the system can be suitable for multi-scene use, such as emergency control, accident disaster rescue, field medical treatment, special population on-site medical treatment, routine inspection of village health houses and community health stations, hospital emergency treatment, emergency use of other departments of hospitals, household self-inspection and the like, breaks through the limitation that the traditional human engineering method and instrument method can only be used in a fixed manner in a laboratory, changes the scene mode of medical inspection, caters to the policies of internet and medical treatment, and brings great social value for emergency rescue, mobile medical treatment, household pre-inspection of common heating patients, antibiotic management and the like. The social value brought by the change of the use scene is not realized in the blood cell detection of the current human engineering method and the instrument method.

Drawings

FIG. 1(a) is a top view of a sample processing plate in one embodiment of the present application;

FIG. 1(b) is a side view of a sample processing plate in one embodiment of the present application;

FIG. 2(a) is a side view of an enlarged counting plate according to a first embodiment of the present application;

FIG. 2(b) is a top view of an enlarged counting plate according to a first embodiment of the present application;

FIG. 2(c) is a side view of an enlarged counting plate in a second embodiment of the present application;

FIG. 2(d) is a top view of an enlarged counting plate according to a second embodiment of the present application;

FIG. 3(a) is a schematic structural view of a blood collection device according to an embodiment of the present application;

FIG. 3(b) is a first state diagram of the blood collection device according to one embodiment of the present application;

FIG. 3(c) is a second state diagram of the blood collection device according to one embodiment of the present application;

FIG. 4 is a block diagram of a blood cell detector according to an embodiment of the present application;

FIG. 5 is a schematic view of the internal structure of a blood cell detector according to an embodiment of the present application;

fig. 6 is a schematic structural diagram of a data analyzer according to an embodiment of the present application.

List of parts and reference numerals:

101, amplifying a counting plate; 1011 a main body panel; 1012 lenses;

1013 grooves; 1014 through holes;

102 a sample processing plate; 1021 slide 1022 sample wells;

1023 supporting; 1024 coverslips;

200 blood collectors; 201, a pipe body; 202, sealing and plugging;

203 automatic quantitative blood collection tube; 204 sample adding tube; 205 a sealing sleeve;

300 a blood cell detector; 301 a housing; 302 an image sensor;

303 a wireless transmitter; 304 an upper cover plate; 305 sample stage;

306 a light transmission hole; 307 light source;

400 a data analyzer; 401 displaying a screen;

1 a metal sealing film; 2, a reagent tube;

Detailed Description

The present application will be described in detail with reference to examples, but the present application is not limited to these examples.

A blood cell analyzer comprises a sample processing plate, an amplifying and counting plate and a blood cell detector; the sample processing plate comprises a glass slide, a sample pool, a supporting column and a cover glass; the amplifying and counting plate comprises a groove for placing the sample processing plate and a biconvex spherical lens fixed in a conical through hole in the center of the groove or an integral outward convex plano-convex lens fixed at the bottom of the main body plate; the blood cell detector comprises an image sensor; the sample processing plate is detachably placed in a proper groove matched with the amplifying and counting plate; the magnifying and counting plate is detachably mounted to the blood cell detector so that the optical image of the blood cell sample magnified by the lens is transmitted to the image sensor.

The blood cell analyzer provided by the application carries out flow control distribution processing to a sample by a sample processing plate, amplifies the blood cell sample through amplification counting, and then transmits an amplified optical image to an image sensor. The sample treatment plate and the amplification counting plate are detachably mounted in the blood cell detector, and the sample amplification counting assembly and the amplification counting plate after detection can be discarded, so that the disposable effect of blood cell analysis is realized, and the reliability of a test result is ensured.

Optionally, the sample processing plate comprises a glass slide, a sample cell, a support member and a cover glass, the cover glass is fixed in the groove along the horizontal direction, and a gap for holding the blood cell sample is formed between the cover glass and the bottom surface of the groove through the support member; a groove matched with the sample processing plate is arranged in the middle area of the amplifying and counting plate, a conical through hole is formed in the main body plate corresponding to the central area of the groove, the major diameter of the main body plate is arranged at the end of the groove, and a biconvex spherical lens is fixed in the conical through hole; or the bottom surface of the main body plate corresponding to the central area of the groove is provided with an integrally exposed plano-convex lens; and a sample processing plate is placed in the groove of the amplification counting plate.

Specifically, the supporting member of the sample processing plate may be a plurality of supporting columns, one end of each supporting column is fixed on the bottom surface of the sample pool, the other end of each supporting column is fixed on the cover glass, a gap for containing the blood sample is formed between the cover glass and the bottom surface of the sample pool, and the blood sample is flattened into a single-cell layer shape through the gap, so that the imaging effect and the accuracy of data analysis are prevented from being influenced by cell stacking. The middle area of the magnifying counting plate is provided with a groove, the shape and the size of the groove are matched with the sample processing plate, the magnifying counting plate can be divided into two types, one type is that a conical through hole is arranged on the main body plate corresponding to the central area of the groove along the vertical direction, the major diameter is arranged at the end of the groove to form an inverted triangular conical through hole, and a biconvex spherical lens is fixed in the conical through hole; the through hole is designed into an inverted triangular cone shape, and has the following functions: the double-convex spherical lens can be supported to be fixed, the small diameter of the double-convex spherical lens is used as a diaphragm of the double-convex spherical lens, the size of the diaphragm influences the brightness of an image, the diaphragm can correct phase difference to influence the resolution of the image, and the diameter and the focal length of the lens determine the magnification. The other type of the magnifying and counting plate is that an integrated plano-convex lens is arranged on the bottom surface of the main body plate corresponding to the central area of the groove, the diameter and the focal length of the plano-convex lens determine the magnification and the resolution, and the plano-convex lens is also provided with a diaphragm.

The lens in the central area of the counting magnifying plate is a micron-sized multi-specification optical magnifier, the magnification is different from 50 times to 500 times, the magnifying plate can replace a biological microscope and is a disposable magnifying plate, the clinical examination standard is met, and the reliability of results is guaranteed. The use of the method is not used in a human method and an instrument method blood fine inspection method.

Optionally, the height of the gap between the cover glass and the bottom surface of the groove along the vertical direction is 0.05-0.12 mm. This gap height may allow the blood sample to be tiled into a better single cell layer.

Specifically, the gap formed between the cover glass and the bottom surface of the groove for containing the blood sample can make the blood sample form a single cell membrane, preferably with a thickness of 0.1 mm. The thickness of clearance is undersized, and the support column is difficult to prepare, and the thickness of clearance is too big, and the cell can coincide.

Optionally, the diameter of the large-diameter end of the through hole is 3-8 mm; the diameter of the small-diameter end of the through hole is 0.3-0.8 mm.

Specifically, the small diameter end of the through hole is equivalent to a diaphragm structure, the diaphragm can improve the brightness of the image, and the diaphragm can correct the phase difference so as to improve the resolution of the image.

Optionally, the diameter ratio of the large diameter end to the small diameter end of the through-hole is 10: 1.

Optionally, the height of the through hole in the axial direction is 1-5 mm. Specifically, when the height of the through hole in the axial direction is 1-5 mm, the size range of the focal length can be met.

Optionally, the spherical lens is a biconvex lens, which can meet the requirement of processing and preparation.

Optionally, the diameter of the biconvex spherical lens is in the range of 0.5-6 mm. Specifically, when the diameter range of the biconvex spherical lens is 0.5-6mm, the range of the magnification factor of 50-500 times can be satisfied.

Specifically, when the thickness of the plano-convex lens along the vertical direction is 0.05-2mm, the diameter range of the plano-convex lens is 0.3-6 mm, the range of the magnification factor of 50-500 times can be met, and the range of the focal length can be met when the thickness of the main body plate is 1-5 mm.

The blood cell detector also comprises a shell, a wireless transmitter and an upper cover plate; the upper surface of the shell is provided with a concave sample table for placing the amplification counting assembly; the sample table is provided with a light hole matched with the lens on the magnifying and counting plate; the image sensor and the wireless transmitter are positioned in a cavity formed by the enclosure of the shell, and the image sensor is electrically connected with the wireless transmitter; the image sensor is matched with the light hole; the upper cover plate is positioned on the upper surface of the shell, and a light source is installed on one surface, facing the shell, of the upper cover plate.

Specifically, the sample stage is matched with the magnifying counting plate, and the magnifying counting plate is inserted on the sample stage during blood analysis.

Optionally, the hemocyte analyzer further comprises a blood collector, wherein the blood collector comprises a tube body, a plug, an automatic quantitative blood collection tube, a sample adding tube and a sealing sleeve; one end of the tube body along the axial direction is opened, and the sealing plug is detachably arranged at one end of the tube body; the automatic quantitative blood collection tube and the sample adding tube are respectively fixed at two ends of the sealing plug along the axial direction, and the automatic quantitative blood collection tube is positioned in the tube body; the automatic quantitative blood collection tube is communicated with the sample adding tube; the seal cover is arranged at one end of the sampling tube. The hemostix has simple structure and convenient use.

Specifically, the application of sample pipe is arranged in adding the blood sample in the body to the enlarged count subassembly, and the seal cover is used for guaranteeing that the blood sample does not receive the pollution.

Optionally, the blood cell analyzer further comprises a data analyzer comprising a data receiver, a data processor and a display screen;

the data receiver receives a data signal in the wireless transmitter;

the data receiver is electrically connected with the data processor;

the data processor is electrically connected with the display screen.

The novel intelligent movable hematology analyzer provided by the application sucks finger blood through a quantitative capillary suction tube (automatic quantitative blood collection tube) which is sterilized in advance and stored in a sealed plastic tube (tube body of a blood collector), then put into another plastic tube (as shown in fig. 3 (c)) with the reagent added in advance, mixed evenly and dripped into the sample cell of the sample processing plate, the sample processing plate is placed in the groove of the magnifying counting plate, the magnifying counting plate is placed in the blood cell detector, the light emitted by the light source lamp in the blood cell detector penetrates through the sample and the magnifying counting plate, the blood cell optical image which is magnified and imaged by the lens at the lower part of the magnifying counting plate is projected on the photosensitive surface of the photoelectric sensor (namely the image sensor), then the optical image is converted into a digital signal through a photoelectric sensor and A/D conversion, and the digital signal is transmitted to a data analyzer of the mobile terminal through a wifi wireless transmitter. The blood cell image is displayed on a screen of the data analyzer in real time, meanwhile, the number and the form of the blood cells and classification data of the white blood cells are analyzed in real time through image algorithm analysis software in the analyzer, the data can be stored in the analyzer, can be wirelessly transmitted to a specific data terminal and can also be printed and reported through a wireless printer, and the analysis result is uploaded to a medical internet cloud platform in real time.

The following describes possible implementations

A wireless handheld intelligent blood cell analyzer uses a blood collector 200, takes finger blood, puts into a pre-filled reagent tube 2, mixing, injecting into a sample processing plate 102, placing the amplifying and counting plate 101 into a blood cell detector 300, the blood cell image is obtained by magnifying and imaging through a lens 1012 in the magnifying and counting plate 101, the optical image is converted into an electric signal through the image sensor 302, the electric signal is converted into a digital signal through a/D conversion, transmitted to the data analyzer 400 through the wireless transmitter 303, and analyzed by using algorithm analysis software installed in the data terminal to obtain a result report of the total number of platelets, red blood cells and white blood cells and the classification of white blood cells, the result report is displayed and stored in the data analyzer 400, the user may read the results directly on the display 401 of the data analyzer 400. The measurement result can be wirelessly printed out to form a report, can be transmitted to other specific terminals through Wifi, can be uploaded to a medical internet cloud platform in real time, and is used by clinicians and a shared data center. The blood cell detector 300 is a cordless and wireless handheld palm type, and the data analyzer 400 may be a mobile terminal such as a mobile phone, a tablet computer, a notebook computer, a mobile microcomputer, etc.

Preferably, the blood sampling device 200 is formed by matching a plastic tube (i.e. a tube body 201) and a plastic plug 202, an automatic quantitative blood sampling tube 203 with a capacity of 0.5-30ul is installed below the plug 202, a sample adding tube 204 is connected to the top of the plastic tube cap, a plastic sealing sleeve 205 with one sealed end is installed on the top of the sample adding tube 204, the automatic quantitative blood sampling tube 203 is made of glass or plastic, the automatic quantitative blood sampling tube 203 can be independently installed on the plug 202, and the automatic quantitative blood sampling tube 203 can also be integrally formed with the plug 202 by using an injection molding process.

Preferably, a diluent, hemolysin, a coloring agent, and the like are added in advance to the plastic tube (i.e., the tube body 201), and the orifice thereof is sealed with the metal sealing film 1.

Preferably, the sample processing plate 102 is made of plastic by injection molding, the sample processing plate 102 includes a glass slide 1021, a concave sample cell 1022 is provided on the upper portion of the glass slide 1021, four support columns 1023 with a height of 0.1mm are provided on the sample cell 1022, virtual thin lines for identifying different areas are engraved in the central area of the bottom of the sample cell 1022, and specifically, the virtual thin lines are drawn by software on the electronic image after optical amplification transmission and are rectangular in shape as an effective range of blood sample cell count. A cover glass 1024 is secured over the support posts 1023. The magnifying and counting plate 101 comprises a main body plate 1011, a recess 1013 is formed on the upper surface of the main body plate 1011, a tapered small hole (i.e. a through hole 1014) is formed on the main body plate 1011 corresponding to the central region of the recess 1013, the aperture range is 0.3-3mm, a lens 1012 is arranged in the middle of the small hole, the diameter range of the lens 1012 is 0.5-6mm, the lens material can be glass, plastic or other transparent materials, the lens can be spherical, and the spherical lens has double convex and plano-convex. In order to prevent cross contamination of cells and cross infection of pathogens and facilitate multi-scene field instant use, the magnifying counting plate 101 is designed into disposable parts with various specifications.

Preferably, in the blood cell detector 300, the light source 307 of the upper cover plate 304 irradiates cells in the blood sample under the cover glass 1024 placed in the sample processing plate 102, the cells are magnified and imaged by the lens 1012 with a magnification of 5-500 times, an optical image of the obtained cells is projected onto the light sensing surface of the image sensor 302, then the optical image is converted into an electrical signal by the sensor, the electrical signal is converted into a digital signal by a/D conversion, the digital signal is sent to the data analyzer 400 by the wireless transmitter 303 for processing, and finally the digital image is converted into a digital image which is displayed on the touch screen (i.e., the display screen 401) of the mobile terminal-data analyzer 400, and medical data processing and analysis are performed by algorithm software.

Preferably, the image sensor 302 is a CCD or CMOS chip.

Preferably, the wireless transmitter 303 employs a wifi or bluetooth module.

Preferably, the data analyzer 400 is a mobile terminal, which may be a mobile phone, a tablet computer, a notebook computer, a mobile microcomputer, or the like, and may be used in windows and android systems, where the windows system is selected, the computer performs medical data processing and analysis on the cells through installed image algorithm analysis software, and the android system is selected, and the mobile phone installs dedicated App software for image algorithm analysis to perform medical data processing and analysis on the cells. And uploading the analysis result to a medical internet cloud platform in real time.

Example 1

FIG. 1(a) is a top view of the sample processing plate in this embodiment, and FIG. 1(b) is a side view of the sample processing plate in this embodiment; fig. 2(a) is a side view of an enlarged counter plate in the present embodiment, fig. 2(b) is a top view of the enlarged counter plate in the present embodiment, fig. 3(a) is a schematic structural view of a blood collection device in the present embodiment, fig. 3(b) is a first usage state diagram of the blood collection device in the present embodiment, fig. 3(c) is a second usage state diagram of the blood collection device in the present embodiment, fig. 4 is a schematic structural view of a blood cell detector in the present embodiment, fig. 5 is a schematic internal structural view of the blood cell detector in the present embodiment, fig. 6 is a schematic structural view of a data analyzer in the present embodiment, and the present embodiment will be specifically described below with reference to fig. 1 to 6.

As shown in fig. 1(a) and 1(b), the blood cell analyzer includes a sample processing board 102 (collectively referred to as 102), the sample processing board 102 includes a slide 1021, a sample cell 1022, 4 support posts 1023, and a cover glass 1024, the 4 support posts 1023 are respectively located at 4 edge positions of the sample cell 1022, the square cover glass 1024 is fixed on the support posts 1023 in a horizontal direction, and a gap for holding a blood sample is formed between the cover glass 1024 and the bottom surface of the sample cell 1022.

As shown in fig. 2(a) and 2(b), the blood cell analyzer further includes an enlarged counting plate 101 (generally designated as 101); the magnifying and counting plate 101 comprises a main body plate 1011 and a lens 1012, wherein a square (or round) groove 1013 is provided on the main body plate 1011, a tapered through hole 1014 is provided on the bottom surface of the groove 1013 in the vertical direction, the lens 1012 is disposed in the through hole 1014, the large diameter end of the through hole 1014 is close to the groove 1013, and the small diameter end of the through hole 1014 is far from the groove 1013.

The height of the gap in the vertical direction is 0.1mm, the diameter of the large diameter end of the through hole 1014 is 3mm, the diameter of the small diameter end of the through hole 1014 is 0.3mm, the height of the through hole 1014 in the axial direction is 2mm, and the lens 1012 is a biconvex lens having a diameter of 0.8 mm.

As shown in fig. 3(a), the blood cell analyzer further includes a blood sampling device 200 (generally designated as 200), which includes a tube body 201, a plug 202, an automatic quantitative blood sampling tube 203, a sample addition tube 204, and a sealing sleeve 205; one end of the tube body 201 along the axial direction is opened, and the sealing plug 202 is detachably arranged at one end of the tube body 201 to form a sealing effect; the automatic quantitative blood collection tube 203 and the sample adding tube 204 are respectively fixed at two ends of the sealing plug 202 along the axial direction, and the automatic quantitative blood collection tube 203 is positioned in the tube body 201; the automatic quantitative blood collection tube 203 is communicated with the sample adding tube 204; a gland 205 is mounted at one end of the loading tube 204.

As shown in fig. 4 and 5, the blood cell analyzer further includes a blood cell detector 300 (generally designated as 300), the blood cell detector 300 including a housing 301, an image sensor 302, a wireless transmitter 303, and an upper cover 304; a concave sample table 305 for placing the amplifying and counting plate 101 is arranged on the upper surface of the shell 301; the sample table 305 is provided with a light-transmitting hole 306 matched with the conical through hole 1014 on the amplifying counting plate 101; the image sensor 302 and the wireless transmitter 303 are positioned in a cavity formed by the enclosure of the shell 301, and the image sensor 302 is electrically connected with the wireless transmitter 303; the image sensor 302 fits into the light-transmissive hole 306; the upper cover 304 is positioned on the upper surface of the housing 301, and a light source 307 is attached to a surface of the upper cover 304 facing the housing.

As shown in fig. 6, the blood cell analyzer further includes a data analyzer 400, the data analyzer 400 including a data receiver, a data processor, and a display screen 401; the data receiver receives a data signal in the wireless transmitter; the data receiver is electrically connected with the data processor; the data processor is electrically connected to the display 401.

Example 2

Fig. 2(c) is a side view of the enlarged counting plate in the present embodiment, and fig. 2(d) is a top view of the enlarged counting plate in the present embodiment. The following description will be made with reference to fig. 2(c) and 2 (d).

In the present embodiment, another structure of the magnifying and counting plate 101 is provided, as shown in fig. 2(c) and 2(d), a recess 1013 is provided on the main body plate 1011 of the magnifying and counting plate 101, and an integral convex-flat lens 1012 protruding outward is provided on the bottom surface of the main body plate 1011 corresponding to the central region of the recess 1013.

In this embodiment, the thickness of the plano-convex lens in the vertical direction is 0.3mm, the diameter range of the plano-convex lens is 0.5-1.0mm, and the thickness of the main body plate is 1.5 mm.

The following describes a method of using the blood cell analyzer according to the present embodiment.

Firstly, blood is collected by the blood collector 200, and the specific operations are as follows: puncturing a ring finger sterilized by alcohol in advance with a commercial blood taking needle, wiping off a first drop of blood, slightly squeezing finger blood, unscrewing a seal plug 202 on a tube body 201 of the blood collector 200, sucking the blood with an automatic quantitative blood collecting tube 203, then placing the blood into a reagent tube 2 with a metal sealing film 1 torn off, as shown in fig. 3(b), wherein the specification of the reagent tube 2 is the same as that of the tube body 201 of the blood collector 200, tightly covering the seal plug 202, and as shown in fig. 3(c), slightly inverting and mixing the reagent tube 2.

The mixed blood sample (i.e., blood cell sample) is then transferred into the sample processing plate 102. The specific operation is as follows: the gland 205 above the loading tube 204 is removed, the reagent tube 2 is then inverted, the reagent tube 2 is squeezed, the diluted blood at the front end of the loading tube 204 is discarded, and a drop of the diluted blood is dropped into the sample reservoir 1022 of the sample processing plate 102 until the gap below the cover glass 1024 is filled with the diluted blood sample.

Finally, the blood sample is subjected to a test analysis. The specific operation is as follows: the sample processing plate 102 is placed in the groove 1013 in the enlarged counting plate 101, then the enlarged counting plate 101 is placed in the sample stage 305 in the blood cell detector 300, the upper cover plate 304 of the blood cell detector 300 is closed, the power switch of the blood cell detector 300 is turned on, the power switch of the data analyzer 400 is turned on at the same time, it is confirmed that the blood cell detector 300 and the data analyzer 400 are connected wirelessly, at this time, the image of the blood cells is displayed on the display screen 401, and the data analyzer 400 automatically analyzes the cell number, the cell shape and the leukocyte classification result through the built-in algorithmic analysis software. The result is automatically stored in the data analyzer 400, and can also be transmitted to a specific data terminal or a report can be printed out through Wifi, and the analysis result is uploaded to the medical internet cloud platform in real time.

Although the present application has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the application.

Claims

1. A blood cell analyzer, comprising a sample processing plate, an amplifying and counting plate, and a blood cell detector;

the sample processing plate is used for containing a blood cell sample;

the magnifying counting plate comprises a lens;

the blood cell detector includes an image sensor;

2. The hematology analyzer of claim 1, wherein the sample processing plate includes a slide, a support, and a cover slip;

a concave sample pool is arranged on the glass slide;

the cover glass and the support piece are both positioned in the sample pool, and a gap for containing the blood cell sample is formed between the cover glass and the bottom surface of the sample pool through the support piece.

3. The hematology analyzer of claim 2, wherein the height of the gap in the vertical direction is 0.05-0.12 mm.

4. The hematology analyzer of claim 1, wherein the enlarged count plate further comprises a body plate;

the lens is fixed below the groove through the main body plate.

5. The hematology analyzer of claim 4, wherein the main body plate is provided with a tapered through hole in vertical direction, the tapered through hole is communicated with the groove, the lens is a biconvex spherical lens, the biconvex spherical lens is fixed in the through hole, and the through hole is gradually reduced in diameter in vertical direction.

6. The hematology analyzer of claim 5, wherein the diameter of the large diameter end of the through hole is 3-8 mm; the diameter of the small-diameter end of the through hole is 0.3-0.8 mm; the height of the through hole along the axial direction is 1-5 mm;

the diameter range of the biconvex spherical lens is 0.5-6 mm.

7. The hematology analyzer of claim 4, wherein the lens is a plano-convex lens, a planar end of the plano-convex lens is fixed to an outer bottom surface of the main body plate, and a convex end of the plano-convex lens is close to the cell detector.

8. The hematology analyzer of claim 7, wherein the thickness of the plano-convex lens in the vertical direction is 0.05-2mm, and the diameter of the plano-convex lens is in the range of 0.3-6 mm; the thickness of the main body plate is 1-5 mm.

9. The blood cell analyzer of claim 1, wherein the blood cell detector further comprises a housing, an upper cover plate, and a wireless transmitter;

the image sensor is matched with the light hole;

10. The hematology analyzer of claim 1, further comprising a blood sampler, the blood sampler comprising a tube body, a plug, an automatic quantitative blood collection tube, a sample addition tube, and a sealing sleeve;

the seal sleeve is arranged at one end of the sample adding pipe;

the blood cell analyzer further comprises a data analyzer, wherein the data analyzer comprises a data receiver, a data processor and a display screen;

the data receiver receives a data signal in the wireless transmitter;

the data receiver is electrically connected with the data processor;

the data processor is electrically connected with the display screen.