CN108508006A - A kind of portable detector - Google Patents
A kind of portable detector Download PDFInfo
- Publication number
- CN108508006A CN108508006A CN201810243455.4A CN201810243455A CN108508006A CN 108508006 A CN108508006 A CN 108508006A CN 201810243455 A CN201810243455 A CN 201810243455A CN 108508006 A CN108508006 A CN 108508006A
- Authority
- CN
- China
- Prior art keywords
- suction nozzle
- sample
- fixed
- inspection body
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7769—Measurement method of reaction-produced change in sensor
Abstract
The present invention relates to detection technique fields.A kind of portable detector, including a liquid-transfering device, a suction nozzle；Liquid-transfering device is equipped with the suction nozzle mounting portion for installing suction nozzle, and suction nozzle mounting portion is detachably connected with suction nozzle；There is a cavity for accommodating sample in suction nozzle；The both ends of suction nozzle are equipped with opening, and one end open is the liquid sucting port for importing sample, and another end opening is the installing port for connecting suction nozzle mounting portion, and liquid sucting port is connected with cavity with installing port；An inspection body is fixed in cavity, one kind can react when sample submerges and cause to examine body or the changed inspection body of sample chromatogram.It can directly react into the sample inside suction nozzle with body is examined, sample instilled into the step for examining test paper again after eliminating operator's sampling.Suction nozzle of the present invention is disposable, can reduce the probability of sample cross contamination.
Description
Technical field
The present invention relates to detection technique fields, and in particular to a kind of optical information for capableing of analytical control body or sample solution
The detection device of situation of change.
Background technology
Real-time field quick detection has important application valence in fields such as medical diagnosis, food inspection, environment measurings
Value.To realize real-time field quick detection, a variety of detection methods and technology have been produced at present.However, utilizing current inspection
Survey means go the target for realizing highly sensitive, quick pinpoint accuracy detection, and there are still some problems to be solved among these.
Test paper detection method will indicate that the markup reagent of determinand is loaded into filter paper using filter paper as medium.Test paper detects
Method be widely used in biological metabolism molecule (predetermined substance in such as mankind's urine, blood), immune molecule, remains of pesticide (such as cereal,
Pesticide residue in fruit, processed food), the detections of the substances such as environmental contaminants (such as chlorine residue, phenol, cyanide).
Common urinalysis test paper light weight, fragility, make troubles to transport, storing.Sample is lighted on test paper
Position it is improper will cause to examine reaction that cannot be normally carried out, to keep measurement result inaccurate.In addition, this urinalysis examination
Paper is often exposed in external environment, and checkout procedure is also in this way, this will further decrease the accuracy of inspection result.To sum up institute
It states, this test paper detecting method is limited.
When testing using urinalysis test paper, it need to ensure that sample uniformly, synchronously penetrates into the test section of test paper, into
And ensure to carry out while reaction.And under the external environment detected at the scene, usually there is sample cannot equably land on examination
The case where paper, causes test section completely cannot equably obtain the infiltration of sample.
Small-sized uroscopy instrument is common in domestic consumer, can automatically analyze urinalysis test paper.Multiple inspection items will be contained
The test strips immersion urine sample to be measured of mesh (i.e. multiple Examination regions) is taken out, drains extra urine.Then, test strips are put into urine
It examines in instrument, can realize the Multiple components in urine sample, such as the inspection of protein, leucocyte, acetoacetate, pH projects.
The limitation of examined scheme, this small-sized uroscopy instrument are only capable of realizing the semi-automation of detecting step.
Full automatic urine analyzer is common in various big hospital, utilizes automatic drip sample method and array analysis test paper, it is only necessary to grasp
Author places the test tube and test paper for filling sample, and a key can complete the detection of Multiple components in sample, moreover it is possible to realize that batch is examined
It tests.However sample remains significant period of time in the containers such as sampling cup or test tube, causes wherein before being detected
Volatile matter be lost in.Meanwhile analytical instrument will also be polluted by volatile matter in sample.In addition, big in sample volatile matter
In the environment for measuring aggregation, the physically and mentally healthy of operator also will be by a degree of threat.Full automatic urine analyzer there is also
It is expensive, to the higher disadvantage of operator's professional experiences level requirement.
In addition to this, although above-mentioned fully-automatic analyzer uses disposable analysis test paper, loading analysis test paper
Paper box and grain-clamping table still have by sample infiltration, pollution possibility, be used for a long time, can have the hidden danger of cross contamination.
To avoid cross contamination, this full automatic urine analyzer from usually providing sampling needle cleaning function for oneself, it is ensured that after adopt
The sample of collection is not interfered by the sample of last time acquisition.But this cleaning step can often remain a small amount of clear in sampling needle
Washing lotion, the sample acquired after causing are diluted, to influence the accuracy of testing result.
There presently does not exist a kind of portable equipment that can detect pesticide residue, that is, only in the laboratory of profession
The interior detection that could realize pesticide residue.When test in laboratory pesticide residue, it is desirable that drip sample no cross contamination
On test paper.However, the step is usually happened in the environment of opposing open, this by influence analysis result accuracy and can
Repeatability.
To realize that the function of many kinds of substance in synchronous detection sample, a test paper often include multiple Examination regions, can distinguish
It reacts with a variety of determinands.This may cause to interfere with each other between adjacent test area, cause result inaccurate.
Invention content
The object of the present invention is to provide a kind of portable detectors, solve above at least one technical problem.
Following technical scheme may be used to realize in technical problem solved by the invention：
A kind of portable detector, which is characterized in that including a liquid-transfering device, a suction nozzle；
There is a cavity for accommodating sample in the suction nozzle；
By the liquid-transfering device, sample collection to suction nozzle internal cavities or is discharged from；
It is fixed with an inspection body in the cavity, inspection body or sample when examining body to mix with sample to react
Optical information changes；
By the liquid-transfering device, after the sample of designated volume enters suction nozzle internal cavities, inspection body can be submerged；
By the method for range estimation or optical detection, the optical information of body is examined in detection；
The situation of change that bulk optics information is examined by investigation achievees the purpose that detect designated substance in sample.
The liquid-transfering device is equipped with the suction nozzle mounting portion for installing the suction nozzle, the suction nozzle mounting portion and the suction
Mouth is detachably connected；
The both ends of the suction nozzle are equipped with opening, and an opening is the liquid sucting port for importing sample, another opening
For the installing port for connecting suction nozzle mounting portion, the liquid sucting port is connected with the cavity with the installing port.Into suction nozzle
Internal sample can directly react with body is examined, this step of test paper is examined in sample instillation again after eliminating operator's sampling
Suddenly.Meanwhile sample to be tested is sealed in reacting for body is examined in suction nozzle, therefore the volatilization of substance, inspection body in sample to be tested
The oxidation on surface is limited, to improve testing result stability, operating environment safety.The present invention is used
Suction nozzle be it is disposable, the probability of sample cross contamination will reduce.Further, since the volatilization of sample is inhibited,
Therefore those samples with special odor, such as urine unhappy can be mitigated to what operator brought.
Using liquid-transfering device by inside sample collection to suction nozzle, spy occurs with sample solution using the inspection body inside suction nozzle
Opposite sex reaction, the idiosyncrasy can make the optical information of inspection body or sample change.According to optical information variation to reaction
Situation is analyzed, to realize the qualitative and quantitative analysis of sample, reach medical diagnosis, food inspection, environment measuring mesh
's.For the present invention compared with existing small-sized test paper detection, operation is more easy, more efficient；With large-scale test paper detection device
It compares, can realize the real-time detection at scene.
It can be as follows for the structure design of inspection body：
It is described that body is examined to contain one or more kinds of reaction reagents；
From different test substances metachromasia can occur for different reaction reagents；So as to be examined to many kinds of substance
It surveys.
As a kind of scheme, examines body to be provided with reference area, color comparator is provided for detection zone；
The suction nozzle cavity inside contains an inspection body frame, the three-dimensional structure for examining body frame, can fixed inspection body,
Ensure to be connected to from liquid sucting port to installing port inside suction nozzle simultaneously.
As a kind of scheme, the inspection body is a test paper；
The test paper includes reaction reagent；
The reaction reagent is the reaction reagent for metachromasia to occur with sample；
The inspection body includes one or more test paper detection zones；
The inspection body includes reference area, and the test paper to react front and back provides color comparator.
It includes but not limited to pH test paper, urinalysis test paper, enzyme linked immunoassay test paper, ambient water or gas point to examine body
Analysis test paper, pesticide test paper quantitatively survey chlorine test paper, surfactant residual test paper.
The inspection body can be planar, central axis one non-90 degree of composition for examining plane and suction nozzle where body
Angle.Preferably, one 15 °~35 ° of angle is constituted.Convenient for observation.
The inside that body inclination will be examined to be fixed on suction nozzle, rational viewing angle is provided for human eye, convenient for range estimation reaction knot
Fruit, while also allowing to carry out optical detection using optical sensor.
Alternatively, the inspection body includes one or more spheres, cylinder or polyhedron；
The sphere, cylinder or polyhedron include reaction reagent；
The reaction reagent is the reagent for metachromasia to occur with sample；The inspection of sphere, cylinder or polyhedral
Physical efficiency is tested to come into full contact with sample in suction nozzle, so uniformly, fully absorb sample, promote reaction generation；The inspection body
Include with reference to body, sphere, cylinder or polyhedron to react front and back provide color comparator.
" color " of the present invention refers to " optical information "；" discoloration " refers to that " optical information becomes
Change ", color (visible spectrum) variation that can be observed with the naked eye can use the colorimetric determination of range estimation, alternatively, no
The variation (the subtle variation of visible light or the variation of non-visible) for the optical information that can with the naked eye distinguish, can be used light splitting light
Degree method or Color images detecting method are detected.According to the difference of detection method, the construction of liquid-transfering device is also different, suction nozzle
Structure, material feature are also different.
The liquid-transfering device includes one for controlling sample sucking or the syringe of the suction nozzle, the syringe being discharged
It is inside equipped with a hollow chamber, a moveable piston is equipped in the hollow chamber, the movement of the piston is used for as sample
Sucking or discharge drainage；
The side of the piston is fixed with a drive shaft, and the drive shaft can drive piston to be slided in hollow chamber；
Side of the syringe far from the drive shaft is equipped with the communicating passage be connected with the cavity of suction nozzle；
The communicating passage is connected with the chamber far from the drive shaft side in syringe；
The liquid-transfering device further includes a shell, and the syringe is fixed in the shell, and is opened up on the shell
There are the opening with the communicating passage mutual conduction, side of the drive shaft far from the communicating passage to stretch out the shell；
In on the shell, the opening is located at the suction nozzle mounting portion, the suction nozzle installation for the suction nozzle installation position
It portion can be with the suction nozzle dismantling connection.
There is drive shaft rebounding mechanism in the shell, when drive shaft is not by the power from hull outside, rebounding mechanism energy
Drive shaft is restored automatically to initial position.
After installing suction nozzle, chamber of the syringe far from the drive shaft side can be connected with suction nozzle inner space, and sliding is lived
Sample to be tested is drawn into suction nozzle by plug, the inspection body into the sample submergence suction nozzle inside suction nozzle, anti-with examining body to occur
It answers, the optical information for examining body or sample solution is caused to change, the determinand in sample is detected to reach
Purpose.
When the present invention uses optical detection, the liquid-transfering device of use includes light source, fluorescence detector, measure loop etc.
Structure；When detection method of the present invention using range estimation, the liquid-transfering device of use does not include above structure.
When the present invention uses optical detection, a light source, an optical sensor are fixed on the suction nozzle mounting portion；
The light emission direction of the light source is towards the cavity of the suction nozzle, and the induction direction of the optical sensor is also towards institute
State the cavity of suction nozzle.
The light source is used for providing illumination or excitation spectrum, optical sensor be used for detecting the reflected light for carrying out self-checking body or
The transmitted light of sample solution.
The optical sensor includes color 2 D array Charged Couple original paper (CCD)；
The color 2 D array CCD, which is used for detecting, examines body and the optical information before and after example reaction；
Specifically, when optical sensor is color 2 D array CCD, the image for examining body can be acquired, obtains and examines
The optical information of body different location, i.e., the intensity signal of R (red), G (green), B (indigo plant) at different location.Examine body and example reaction
It is front and back, the light intensity of comparison test body each detection zone R, G, B, to detect the predetermined substance in sample.
Image detection is able to detect that the slight change for examining bulk optics information, and high sensitivity, precision are good；Image detection
Method can also be investigated simultaneously examines detection zone different on body, passes through the multi-component in the quick judgement sample of image procossing.
Two light-transmitting openings are further opened on the shell, two light-transmitting openings are respectively used to the export of light source and light imports
Optical sensor；
The light source is fixed on the optical sensor in the shell.
When the present invention uses optical detection, it is fixed on the outer wall of the shell for showing that optical sensor detects
The indication mechanism of the test result arrived；
Convenient for passing through indication mechanism, the optical information of body or sample solution is examined in display.
When the present invention uses optical detection, there are measure loop and power supply in the shell；
The measure loop is used for for collecting information of the processing from optical sensor, the power supply as light source, detection
Device, measure loop, indication mechanism provide electric power；
The outer wall of shell is equipped with operating mechanism, and the operating mechanism is for controlling measure loop.
When the present invention uses optical detection, there is radio transmission apparatus in the shell.It is set by the wireless transmission
It is standby, the collected information of optical sensor is transmitted in mobile phone, wearable device or computer terminal.
When optical detection of the present invention, including a mobile terminal processing routine.The mobile terminal processing routine
It can be used for mobile phone, wearable device or computer terminal, image procossing realized by human-computer interaction, data are shown and data are deposited
Storage.
A radio transmitting device is fixed in the shell；The radio transmitting device is for collecting the letter from measure loop
Breath, and send it in mobile phone, wearable device and computer terminal.
A kind of portable detector, including a suction nozzle.During detecting sample, the liquid sucting port of the suction nozzle is located at suction nozzle
Lower end, installing port is located at the upper end of suction nozzle；
The liquid sucting port of suction nozzle is micromillimetre size, narrow upper width under internal diameter；
When ensureing containing liquids sample, sample does not drip due to gravity；It can ensure suction nozzle with upper width narrow at present
Volume.
Preferably, suction nozzle internal diameter is invariable after being gradually incremented by from bottom to up.Convenient for examine body suction nozzle constant inner diameter not
It is housed at change.
The suction nozzle can have any shape.
As a kind of reference scheme, the circular in cross-section or polygon of the suction nozzle.
When the cross section of suction nozzle be in polygon, that is, the suction nozzle upper end be in prism-shaped, the lower end of the suction nozzle
Portion is in internal diameter gradual incremental pyramid-shaped from bottom to up, the seamed edge number phase of the suction nozzle upper end and the suction nozzle lower end
Together.
It is that suction nozzle can be designed as follows for estimating：
As a kind of reference scheme, the suction nozzle is suction nozzle made of transparent material.Convenient for passing through outside detection suction nozzle
It estimates to assess the inspection body or sample solution color, depth state.
The cross section of the suction nozzle is in polygon.
When the cross section of suction nozzle be in polygon, that is, the suction nozzle upper end be in prism-shaped, the lower end of the suction nozzle
Portion is in internal diameter gradual incremental pyramid-shaped from bottom to up, the seamed edge number phase of the suction nozzle upper end and the suction nozzle lower end
Together.
The radius of curvature of suction nozzle outer wall is infinity under this structure, can eliminate the outer wall of suction nozzle bending to examining body
Dispersion interaction.The suction nozzle of this structure obtains more accurate result convenient for ocular estimate.
Further, preferably, the wall thickness any place of suction nozzle is equal.Convenient for eliminating dispersion, obtained when using ocular estimate
Result it is more accurate.
Suction nozzle for optical sensor detection can be designed as follows：
As a kind of reference scheme, the outer wall of suction nozzle can also have shade function.To prevent the interference of ambient light, ensure
Accuracy of detection when being detected using optical sensor.
The present invention can be used for the real-time, existing of the substances such as biological metabolism molecule, immune molecule, remains of pesticide, environmental contaminants
Field, automatic detection.Correspondingly, examine body include but not limited to pH test paper, urinalysis test paper, enzyme linked immunoassay test paper,
Ambient water or gas analysis test paper, pesticide test paper, quantitatively survey chlorine test paper, surfactant residual test paper etc..Specifically：
The present invention can be used for pH detections, wherein it is pH test paper to examine body.PH test paper is to refer to indicate sample by color
A kind of test paper of product pH value, such as the examination containing acid-base indicators such as bromothymol blues (Bromothymol Blue, BTB)
Paper, when sample is alkalinity, test paper is in blue-green, is taken on a red color when acid.BTB indicator can be additionally used in the quantitative detection of determinand.
The present invention can be used for urinalysis, and inspection body is a kind of urinalysis test paper.Urinalysis test paper generally comprises more
Kind detection reagent, so as to be detected to many kinds of substance.The substance that can be detected includes but not limited to mainly leucocyte, nitrous
Hydrochlorate, urobilinogen, protein, pH, blood, proportion, ascorbic acid, ketoboidies, bilirubin, glucose sugar.
The present invention can be used for enzyme linked immunosorbent detection.Suction nozzle of the present invention can successively acquire sample, reaction reagent respectively.
It examines in body and is fixed with the antibody (antigen) that can be specifically bound with determined antigen (antibody).Sample to be tested is acquired using suction nozzle,
Sample infiltration is set to examine body, then determined antigen (antibody) and antibody (antigen) specific bond in inspection body, reaction balance heel row
Go out sample to be tested.Then, with the antibody (antigen) of suction nozzle acquisition enzyme label, i.e. marker, discharge marker is molten after the completion of reaction
Liquid.Finally, zymolyte, i.e. color developing agent are acquired with suction nozzle, makes the marker on detection body that catalyzing hydrolysis or oxidation occur also
Original reacts and specific color is presented.
The present invention can be used for the quantitative detection of the substances such as phenols in environment measuring, dissolved oxygen, fluoride, cyanide：Reaction
When reagent includes 4-AA, body is examined to be used to measure the concentration of phenol；
When reaction reagent includes at least one of methylene blue, indigo reagent, examine body for during environment measuring
Detect the dissolved oxygen in sample；The chemical reaction of methylene blue and indigo reagent and dissolved oxygen generates coloring matter, measures it
Absorbance is, it can be achieved that quantitative detection to dissolved oxygen；
When reaction reagent includes lanthanum nitrate, examine body for the detection of the fluoride in environment measuring；Lanthanum nitrate and fluorine from
Son reaction generates blue ternary complexes, then can be measured to fluoride by spectrophotometry；Reaction reagent includes alizarin
When sodium sulfonate and zirconates generate red complex, examine body for the detection of the fluoride in environment measuring；Have in sample fluorine from
The period of the day from 11 p.m. to 1 a.m can capture zirconium ion in complex compound, generate colourless fluorination zirconium ion, release the sodium alizarine sulphonate of yellow, utilize mesh
Survey method is taken off by red to the coloration of yellow and standard colorimetric according to solution, to realize the qualitative and quantitative detection of fluoride；
Reaction reagent includes pyridine-pyrazol quinoline ketone, and body is examined to pass through light splitting for the detection of the cyanide in environment measuring
Photometry carries out quantitative detection to cyanide.
The present invention can be used for the detection of organophosphor or carbamates in pesticide residue, and it includes indophenols and indophenols to examine body
Acetic acid esters.Organophosphor or carbamates are inhibited to the activity of acetylcholine esterase in pesticide residue, due to second
Phatidylcholine lipase participates in the balanced reaction of the indophenols and the indophenols acetic acid esters to take on a red color in blue, is with indophenols and indophenols acetic acid esters
Indicator can measure the activity of acetylcholine esterase, to be detected to pesticide residue.
The present invention can be used for free chlorine detection, and it includes N to examine body, when N- diethyl-Isosorbide-5-Nitrae-phenylenediamine (DPD).In sample
Free chlorine reacted with DPD after, absorbance of the free chlorine at 515nm can be surveyed, to reach it is quantitative detection sample chlorine residue mesh
's.
The present invention can be used for surfactant detection, and it includes methylenum careuleum to examine body.Utilize anion surfactant and Asia
First indigo plant forms blue-colored ionic associated matter, and blue-colored ionic associated matter measures its absorbance after chloroform extracts under 625nm light sources,
And then calculate the concentration of anion surfactant.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of the suction nozzle of the present invention；
Fig. 2 is another structural schematic diagram of the suction nozzle of the present invention；
Fig. 3 is a kind of structural schematic diagram of the inspection body frame of the present invention；
Fig. 4 is sectional view at X-X in Fig. 3 of the present invention；
Fig. 5 is another structural schematic diagram of the suction nozzle of the present invention；
Fig. 6 is another structural schematic diagram of the suction nozzle of the present invention；
Fig. 7 is another structural schematic diagram of the suction nozzle of the present invention；
Fig. 8 is another structural schematic diagram of the suction nozzle of the present invention；
Fig. 9 is another structural schematic diagram of the suction nozzle of the present invention；
Figure 10 is a kind of structural schematic diagram of liquid-transfering device of the present invention；
Figure 11 is a kind of structural schematic diagram that the present invention is equipped under the liquid-transfering device detection sample state of suction nozzle；
Figure 12 is that the present invention does not detect a kind of structural schematic diagram under sample state using Figure 10 structures；
Figure 13 is another structural schematic diagram of liquid-transfering device of the present invention；
Figure 14 is that the present invention does not detect a kind of structural schematic diagram under sample state using Figure 13 structures.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Conjunction is specifically illustrating that the present invention is further explained.
Referring to Fig.1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7, Fig. 8, Fig. 9, Figure 10, Figure 11, Figure 12, Figure 13, Figure 14, it is a kind of
Portable detector, including a liquid-transfering device 12, a suction nozzle 2；Liquid-transfering device 12 is equipped with the suction nozzle for installing suction nozzle 2 and installs
Portion, suction nozzle mounting portion are detachably connected with suction nozzle 2；There is a cavity for accommodating sample in suction nozzle 2；The both ends of suction nozzle 2 are equal
Equipped with opening, one end open is the liquid sucting port 4 for water conservancy diversion sample, and another end opening is the installation for connecting suction nozzle mounting portion
Mouth 6, liquid sucting port 4 is connected with cavity with installing port 6；An inspection body 8, energy when sample is mixed with inspection body 8 are fixed in cavity
It enough reacts, causes that the optical information of body 8 or sample is examined to generate variation.By liquid-transfering device 12, sample collection is arrived
Suction nozzle internal cavities are discharged from.
It can directly react, saved for operator after sampling again by sample with body 8 is examined into the sample inside suction nozzle 2
Instill the step for examining body 8.Meanwhile sample to be tested is sealed in reacting for body 8 is examined in suction nozzle, therefore in sample to be tested
The volatilization of substance examines the oxidation on 8 surface of body to be limited, to improve the stability of testing result, the peace of operating environment
Quan Xing.Suction nozzle 2 of the present invention is disposable, and the probability of sample cross contamination will reduce.Further, since sample
Volatilization inhibited, therefore those samples with special odor, such as urine unhappy can also be obtained to what operator brought
To mitigation.Using liquid-transfering device 12 by inside sample collection to suction nozzle 2, sent out with sample solution using the inspection body 8 inside suction nozzle 2
Raw specific reaction, the idiosyncrasy can make the optical information of inspection body 8 or sample change.Utilize the variation of optical information
Response situation is analyzed, to realize the qualitative and quantitative analysis of sample, reaches medical diagnosis, food inspection, environment measuring
Purpose.For the present invention compared with existing small-sized test paper detection, operation is more easy, more efficient；It is detected with large-scale test paper
Equipment is compared, and can realize the real-time detection at scene.
A kind of portable detector, including a suction nozzle 2.During detecting sample, liquid sucting port 4 is located at the lower end of suction nozzle,
Installing port 6 is located at the upper end of suction nozzle；The liquid sucting port of suction nozzle is micromillimetre size, narrow upper width under internal diameter, circular in cross-section or more
Side shape.Preferably, suction nozzle internal diameter is invariable after being gradually incremented by from bottom to up.Convenient for examining body at the constant place of suction nozzle constant inner diameter
It is accommodating.
The suction nozzle 2 can have any shape.
As a kind of reference scheme, the circular in cross-section or polygon of suction nozzle.
When the cross section of suction nozzle is in polygon, that is, the upper end of suction nozzle is in prism-shaped, and the lower end of suction nozzle is in internal diameter
Gradually incremental pyramid-shaped, suction nozzle upper end are identical as the seamed edge number of suction nozzle lower end from bottom to up.
It is that suction nozzle can be designed as follows for estimating：
As a kind of reference scheme, suction nozzle is suction nozzle made of transparent material.Convenient for passing through range estimation outside detection suction nozzle
Body or sample solution color, depth state are examined to assess.
The cross section of suction nozzle is in polygon.
When the cross section of suction nozzle is in polygon, that is, the upper end of suction nozzle is in prism-shaped, and the lower end of suction nozzle is in internal diameter
Gradually incremental pyramid-shaped, suction nozzle upper end are identical as the seamed edge number of suction nozzle lower end from bottom to up.
The radius of curvature of suction nozzle outer wall is infinity under this structure, can eliminate the outer wall of suction nozzle bending to examining body
Dispersion interaction.The suction nozzle of this structure obtains more accurate result convenient for ocular estimate.
Further, preferably, the wall thickness any place of suction nozzle is equal.Convenient for eliminating astigmatism, obtained when using ocular estimate
Result it is more accurate.
Suction nozzle for optical sensor monitoring can be designed as follows：
As a kind of reference scheme, the outer wall of suction nozzle can also have shade function.To prevent the interference of ambient light, ensure
Accuracy of detection when being detected using optical sensor.
A kind of portable detector, including a suction nozzle 2, the suction nozzle 2 is built-in to examine body 8.The built-in suction nozzle 2 for examining body 8
Application method be, using liquid-transfering device 12 drain, so that sample is entered inside suction nozzle 2, and submerge examine body 8.It is waited in sample
It surveys object to react with the reaction reagent 10 contained by body 8 is examined, after reaction balance, suction nozzle 2 is discharged in sample.Body 8 is examined in detection
The optical information situation of upper reaction reagent, by the result with standard with reference to compared with, to reach qualitative and quantitative analysis determinand
Purpose.Detection method of the present invention further includes that sample is retained in inside suction nozzle 2, detects sample solution after reaction balance
Transmitted light situation of change, by the result and standard with reference to compared with, to achieve the purpose that qualitative and quantitative analysis determinand.
It is a test paper containing reaction reagent 10 to examine body 8；Reaction reagent 10 is for metachromasia to occur with sample
Reaction reagent 10.The testing principle involved by body 8 and contained reaction reagent 10 is examined to include：It is existing to be related to spectrum production
Reaction that is raw, disappearing or change, the reaction such as to be changed using color (color reaction) are detected urobilinogen, are sharp
Ferric ion is detected with spectrophotometry (i.e. chromogenic reaction).Examine the optical information of body 8 can by detection
Qualitatively identify test substance, the light intensity by detecting feature light wave can quantitatively detect the concentration of determinand.
It includes one or more detection zone to examine body；Body is examined to contain in different detection zones different anti-
Answer reagent；From different substances metachromasia can occur for different reaction reagents.So as to be detected to many kinds of substance.
It includes but not limited to pH test paper, urinalysis test paper, enzyme linked immunoassay test paper, ambient water or gas to examine body 8
Analysis test paper, pesticide test paper quantitatively survey chlorine test paper, surfactant residual test paper.
As a kind of scheme, it is planar to examine body 8, examines the central axis composition one of the plane and suction nozzle 2 where body
Non-90 degree angle.The inside that body will be examined to be inclined at suction nozzle 2, rational viewing angle is provided for human eye, convenient for range estimation reaction
As a result, also allowing to carry out optical detection using sensor simultaneously.
Alternatively, it is the sphere, cylinder or polyhedron for including reaction reagent 10 to examine body 8；Reaction reagent
10 be the reaction reagent 10 for metachromasia to occur with sample.In spherical, cylindric or polyhedral detection physical efficiency and suction nozzle 2
Sample comes into full contact with, so uniformly, fully absorb sample, promote reaction generation；
When it is spherical, cylindric or polyhedral to examine body 8.It is fixed with inside suction nozzle 2 and examines body frame 11, the inspection
Body frame 11 is used for fixed inspection body 8, while ensureing inside suction nozzle 2 from liquid sucting port 4 to the connection of installing port 6.
As a preferred embodiment, spherical, cylindric or polyhedral inspection body 8, which is fixed on, to be examined on body frame 11.Tool
Body, as shown in Figure 3, Figure 4, body frame 11 is examined to be internally provided with cylindrical passageway 11b and cylindrical recesses 11a.It can be put in cylindrical passageway
Place shape, cylindric or polyhedral inspection body 8 allow sample solution to enter the channel, leaching from close to one end of injection port 4
Body 8 is not examined.Cylindrical recesses 11a can place spherical, cylindric or polyhedral inspection body 8, and sample solution is not easy to submerge position
In the inspection body 8 of cylindrical recesses 11a, therefore the inspection body 8 for being located at cylindrical recesses 11a is used as the object of reference of color reaction.
It examines 11 inside of body frame that can be equipped with one or more cylindrical passageways 11b and cylindrical recesses 11a, can realize many kinds of substance
It detects simultaneously.Specifically, it inside the suction nozzle of Fig. 2, secures a kind of inspection body frame 11 and examines body 8, body 8 is examined to contain including one
There is the sphere of reaction reagent, a kind of substance can be detected；Inside the suction nozzle of Fig. 5, secures a kind of inspection body frame 11 and examine body
8, it examines body 8 to include two spheres containing reaction reagent, two kinds of substances can be detected simultaneously；Inside the suction nozzle of Fig. 6, secure
A kind of inspection body frame 11 and body 8 is examined, it includes more than two spheres containing reaction reagent to examine body 8, can be detected simultaneously
Two or more substances.
It examines body frame 11 to be securable in suction nozzle 2, while ensureing inside suction nozzle 2 from liquid sucting port 4 to the connection of installing port 6.
Specifically, as illustrated in the exemplary embodiment of figure 3, examine the vertical view face of body frame 11 that there is polygon.To examine the polygon top of body frame 11
Point is fulcrum, is fixed in suction nozzle 2；Its boundary retains gap with 2 outer wall of suction nozzle, ensure that liquid sucting port 4 arrives the company of installing port 6
Logical, further ensuring sample solution can immerse inside suction nozzle 2, achieve the effect that body 8 is examined in submergence.Specifically, Fig. 2, Fig. 5
It is internal with imbibition shown in fig. 6 point, secure a kind of inspections body frame 11, inspections body frame is polygonal cylinder shape, in rib and suction nozzle
Wall is in close contact, and plays fixed function, there are gaps with suction nozzle inner wall for cylinder, ensure that connection of the liquid sucting port to installing port.
As one another kind of preferred embodiment, it includes a hollow baton round to examine body, and the inner wall of baton round is filled with micropore material
Expect manufactured adsorption layer, is impregnated with reaction reagent in adsorption layer, baton round is baton round made of transparent material.The one of baton round
Side offers the through-hole flowed into for sample inside baton round.It is reacted with reaction reagent convenient for sample via through holes and develop the color instead
It answers.A specific embodiment is shown in Fig. 7, wherein the spherical inspection body 8 of plastic hollow is fixed with inside suction nozzle 2.
As another preferred embodiment, examine body that the central axial direction of suction nozzle 2 is provided at least two realization samples
The detection zone that product different material measures；Body is examined to contain different reaction reagents in different detection zones.So as to more
Kind substance is detected.Display one includes the specific embodiment of the suction nozzle of tri- different detection zones of 8a, 8b, 8c in Fig. 9.
A kind of portable detector, including a liquid-transfering device 12.Liquid-transfering device 12 include one for control sample sucking or
The syringe 18 of person's discharge, syringe 18 is interior to be equipped with a hollow chamber, can be in the work of chamber indoor moving equipped with one in hollow chamber
The movement of plug 20, piston 20 is used for the sucking for sample or discharge drainage；The side of piston 20 is fixed with a drive shaft 22, can
Driving piston 20 slides in hollow chamber；Side of the syringe 18 far from drive shaft 22 is equipped with communicating passage, and communicating passage makes
Suction nozzle 2 is connected with chamber of the syringe far from 22 side of drive shaft.After suction nozzle 2 is installed, since syringe is connected to suction nozzle, sliding
Piston 20, sample to be tested will be drawn into suction nozzle 2.Further body 8 is examined in submergence, is reacted with body 8 is examined, is caused to examine
The optical information for testing body 8 or sample solution changes, to achieve the purpose that be detected to the determinand in sample.
There is a pressing position 24 in the one end of drive shaft far from piston.Drive shaft 22 has automatic rebound function, works as operator
It presses to pressing position 24, drive shaft 22 pushes piston 20 to be slided to 16 end of communicating passage, if liquid-transfering device 12 is mounted at this time
Suction nozzle 2, and have liquid in suction nozzle 2, then liquid will be discharged in suction nozzle 2；When operator removes the pressure at pressing position 24, driving
Rebound is to initial position automatically for axis 22, if liquid-transfering device 12 is mounted with suction nozzle 2 at this time, and the liquid sucting port of suction nozzle 2 is in liquid
Portion, then liquid will be attracted into suction nozzle 2.
Liquid-transfering device 12 includes a shell 14, and syringe 18 is fixed in shell 14, and is offered and be connected on shell 14
The opening of channel mutual conduction, opening are located at suction nozzle mounting portion；Shell 14 is stretched out in side of the drive shaft 22 far from communicating passage.
The method of range estimation or the method detection predetermined substance of optical detection can be used in a kind of portable detector.
When using the method for range estimation, the application method of portable detector of the present invention is to enter sample collection
Suction nozzle makes sample submergence detection body 8.After several seconds, sample solution in suction nozzle and standard color comparison card are compared, the method for range estimation is passed through
It analyzes sample and examines the response situation of body.Alternatively, after the several seconds, sample solution is discharged, by detection body 8 and standard color comparison card pair
Than analyzing sample by the method for range estimation and examining the response situation of body.With this method, can it is qualitative, semi-quantitatively analyze
Determinand in sample.
When using optical detecting method, the application method of portable detector of the present invention is, by sample collection
Enter suction nozzle, makes sample submergence detection body 8.After several seconds, the optical information for examining body 8 is acquired using optical detection apparatus, by dividing
The situation of change of 8 optical information of body is examined in analysis, is detected to sample composition and content.
When using optical detecting method, portable instrumentation of the present invention includes a liquid-transfering device 12, described
Liquid-transfering device further includes optical detection apparatus.
As a preferred embodiment, the structure of liquid-transfering device 12 and optical detection apparatus can refer to specific reality shown in Fig. 10
Apply example.A light source 26, an optical sensor 28 are fixed on the suction nozzle mounting portion of liquid-transfering device 12；The light emission direction court of light source 26
To the cavity of suction nozzle 2, the induction direction of optical sensor 28 is also towards the cavity of suction nozzle 2.Light source 26 is used for providing illumination or swashs
Luminous spectrum, optical sensor are used for detecting the transmitted light of the reflected light or sample solution of carrying out self-checking body.The characteristics of light source 26 is,
Different according to testing principle used by detection body 8, light source 26 has different spectral signatures, can be exciting light or illumination light
Source, such as light emitting diode or laser diode.Different according to testing principle used by detection body 8, optical sensor 28 has
Different features can be photodiode or the light sensor with optical filter.
The characteristic spectrum of light source 26 is determined by the detection object of liquid-transfering device 12, is visible light wave range, ultraviolet or near-infrared
The spectrum of wave band.The Detection wavelength of optical sensor 28, by the characteristic spectrum of light source 26 and the light of inspection body 8 or sample solution
Information is learned to determine.
Include a measure loop 30 in shell 14, measure loop 30 can be a signal processing module, which connects
Connect optical sensor 28.Measure loop 30 can be collected, handle the detection signal from optical sensor 28, which includes inspection
Test the optical information of body 8 or sample solution.
Two light-transmitting openings are further opened on shell 14, two light-transmitting openings are respectively used to the export of light source 26 and light imports
Optical sensor 28；Light source 26 is fixed on optical sensor 28 in shell 14.
The surface of shell 14 is equipped with operation button 32.Operation button 32 in order to control liquid-transfering device 12 power supply connect or close
Close, control the beginning of optical detection.
Indication mechanism 34 is fixed on the outer wall of shell 14.Indication mechanism 34 is characterized as, can show detection result,
Indicate operation link.
In addition, operation button 32 and indication mechanism 34 can also be integrated in a touch display screen.
Light source 26, optical sensor 28, measuring loop 30, operation button 32 and indication mechanism 34 constitute circuit loop.It moves
Liquid device 12 further includes a power supply, which includes a battery or rechargeable battery.The power supply is used for as light source 26, optical sensor
28, measuring loop 30 and indication mechanism 34 supply electric power.
Specifically, portable detector detection method of the present invention is that suction nozzle 2 is mounted on liquid-transfering device 12,
Calibrate measure loop 30.Sample collection is entered into suction nozzle, makes sample submergence detection body 8.After several seconds, sample is discharged.Utilize LED light source
26 provide illumination for detection body 8, are acquired come the reflected light of self-test body 8 using optical sensor 28.Alternatively, after the several seconds, utilize
LED light source 26 provides light source for sample solution, and the transmitted light from sample solution is acquired using optical sensor 28.Using mould/
Signal from optical sensor 28 is converted to digital signal, the optics letter that analysis optical sensor 28 acquires by number converter
Breath.With this method, can it is qualitative, quantitatively detect determinand in sample solution.
The used detection method of suction nozzle 2 and liquid-transfering device 12 of the present invention is as follows：
First, suction nozzle is mounted in detection device 12.Using syringe 18, certain volume is acquired by liquid sucting port 4
Sample to be tested.As shown in figure 11, after the sample to be tested of the volume enters suction nozzle 2, body 8 is examined in submergence, between sample and inspection body
It reacts.
After reaction balance, sample S is discharged from liquid sucting port 4.(Figure 12) at this time, operator are activated by operation button 32 and are surveyed
Step is measured, then light source 26 is opened, and irradiation examines body 8, the acquisition of optical sensor 28 to carry out the reflected light of self-checking body 8；Alternatively, reaction
After balance, retain sample solution in suction nozzle (Figure 11), operator activates measuring process by operation button 32, then light source 26 is beaten
It opens, sample solution S, optical sensor 28 is excited to acquire the transmitted light from sample solution S.Measuring loop 30, which is collected, comes from optics
The detection signal of sensor 28 carries out analyzing processing to the signal.Measuring loop 30 is equipped with data storage function, can calculate,
Storage carrys out the characteristic optical information of self-checking body 8 or sample solution.Therefore measuring loop 30 is capable of providing data look-up functions.This
Outside, measuring loop 30 can calculate the concentration of determinand by standard working curve, realize quantitative detection.Data search, inspection
Result is surveyed to be indicated by indication mechanism 34.
Alternatively, after reaction balance, sample S is discharged from liquid sucting port 4.(Figure 12) at this time, operator will detect body and standard
Colorimetric object compares, and judges reaction result using the method for range estimation；Alternatively, after reaction balance, retain sample solution (figure in suction nozzle
11), operator compares sample solution S with standard colorimetric object, judges reaction result.At this point, suction nozzle can allow for operator logical
The method of range estimation is crossed to identify the response situation for examining body 8 and sample solution.For example, suction nozzle can be by transparent plastic material system
At.It examines body 8 to be a flat surface shape, is fixed in the chamber inside suction nozzle 2.Examine the axle center structure of the plane and suction nozzle 2 where body 8
At the angle of a non-90 degree, as shown in Figure 8.Inclined inspection body provides rational viewing angle for human eye, convenient for range estimation reaction knot
Fruit；Also liquid-transfering device 12 is allowed to carry out optical detection using optical sensor 28 simultaneously.
When using optical detecting method, as another preferred embodiment, the structure of liquid-transfering device and optical detection apparatus
It can refer to specific embodiment shown in Figure 13.Wherein, it is fixed with a color 2 D array on the suction nozzle mounting portion of liquid-transfering device 12 '
CCD picture pick-up devices 36.Picture pick-up device 36 is fixed in shell 14, can acquire the optical imagery for carrying out self-test body 8.Institute of the present invention
The detection method based on optical imagery stated can obtain the optical information variation of 8 different detection zones of detection body simultaneously.
When detecting, body 8 is provided with reference area or when with reference to body, image detecting method of the present invention can will detect
The optical information of body 8 subtracts reference area or the optical information with reference to body, to more accurately obtain detection bulk optics information
Variation.
Specifically, with reference to figure 13, shell 14 further includes a measure loop 38, and the measure loop 38 can carry out at image
The optical information value for detecting body 8 is subtracted reference zone or with reference to the optical information of body, is led due to metachromasia to obtain by reason
The optical information variable quantity of cause.Measuring loop 38 is equipped with data storage function, can calculate, store and come self-checking body 8 or sample
The characteristic optical information of solution.Therefore measuring loop 38 is capable of providing data look-up functions, provides the reference of test substance concentration
Range.In addition, measuring loop 38 can calculate the concentration of determinand by standard working curve, test substance concentration is provided
Reference value.In specific embodiment shown in Figure 13, counter structure in the function of other structures and the specific embodiment described in Figure 10
Function it is consistent.
In conjunction with the suction nozzle 2 and liquid-transfering device 12, the present invention proposes a kind of method quantitatively detecting remains of pesticide.Utilize agriculture
Medicine residue can inhibit the active principle of acetylcholine esterase, be instruction with pH test paper, and second is hydrolyzed with acetylcholine esterase
The process of phatidylcholine is Indicator Reaction system, indicates, assesses the activity of acetylcholine esterase by the pH of the reaction system, into
One step quantitatively detects the concentration of pesticide residue.Specifically, it is test paper form to examine body 8, and the one side of test paper contains and can react
The other one side of the indicator of pH value, test paper contains acetylcholine and acetylcholine esterase powder.After suction nozzle acquires sample, sample
Body 8 is examined in product submergence, is examined the acetylcholine on body 8 to be catalyzed through acetylcholine esterase and is generated hydrogen ion, causes to examine on body 8
The color of pH indicator changes.The optical information of body 8 is examined using the detection of optical sensor 28 of liquid-transfering device 12.This
Outside, 8 A/D converters can be used, then its saturated light intensity resolution is 255.If in calibration process, optical sensor detection
The bias light intensity of wave arrived is R (red)=60, G (green)=30, B (indigo plant)=40；After the completion of reaction, what optical sensor detected
Light wave intensity is R=60, G=30, B=150, shows that the color change for detecting body 8 is to generate blue.Therefore, pass through analysis
The color change situation for detecting body 8, can analyze and identify determinand.
In conjunction with the suction nozzle 2 and liquid-transfering device 12, the present invention proposes a kind of method quantitatively detecting copper ion in aqueous solution.
Suction nozzle 2 is installed on liquid-transfering device 12, is fixed in suction nozzle 2 and examines body 8, examines and contains 1- (2- pyridylazos) -2- naphthalenes in body 8
Phenol.Containing copper ion concentration it is respectively 0ppm, 0.001ppm, 0.01ppm, 0.1ppm, 1ppm, 10ppm in one group of sample solution,
100ppm.Distinguish pipette samples solution using liquid-transfering device.Before and after pipette samples, inspection is acquired respectively using optical detecting method
Test the optical information of body 8, i.e. R (red), G (green), B (indigo plant), IR (infrared) information.Each sample measures 5 times.Testing result such as table
Shown in 1.In table, the numerical value of each project is the average value after 5 measurements, and " before reaction " is that body 8 is examined to be contacted with sample solution
Preceding optical information, " after reaction " examine body 8 contacted with sample solution after optical information, the optics that " difference " is " after reaction "
Information subtracts the optical information " before reaction ".Data are shown in table 1, examine the optical information of body 8 before and after contacting sample solution
Changed, and as the concentration of copper ion in sample solution increases, the variable quantity of optical information increases.Demonstrate the present invention
The validity of the portable detector of the detection copper ion.
Table 1
In conjunction with the suction nozzle 2 (Fig. 6) and liquid-transfering device 12 ', the present invention proposes a kind of side for quantitatively detecting 11 routine urinalysis
Method.Wherein, the inspection body 8 described in Fig. 6 includes 11 spheres, and the reaction reagent that each sphere contains can indicate following substance respectively
Content：Urobilinogen is occulted blood, Urine proteins, glucose in urine, ketoboidies, bilirubin, albumin, specific gravity of urine, leucocyte, acid-base value, creatinine.
Before and after detection body 8 is contacted with sample to be tested, believed using the optics of 36 acquisition testing body of color 2 D array CCD picture pick-up device
Breath, i.e. R (red), G (green), B (indigo plant) information.Indicate that the optical information of the detection body of every substance is as shown in table 2.Circuit after testing
38 data processing, can be from the term of reference under the measured value and normal body condition that indication mechanism 34 reads indices.Its
In, the content of every substance is：Urobilinogen 2.0mg/dL, 20/μ L that occults blood, Urine proteins 100mg/dL, glucose in urine 250mg/dL, ketone
Body 30mg/dL, bilirubin 1.0mg/dL, albumin 100mg/dL, specific gravity of urine 1.03,100/dL of leucocyte, acidity-basicity ph 7,
Creatinine 150mg/dL.
Table 2
The present invention can be used for the real-time, existing of the substances such as biological metabolism molecule, immune molecule, remains of pesticide, environmental contaminants
Field, automatic detection.Correspondingly, examine body 8 include but not limited to pH test paper, urinalysis test paper, enzyme linked immunoassay test paper,
Ambient water or gas analysis test paper, pesticide test paper, quantitatively survey chlorine test paper, surfactant residual test paper etc..Specifically：
The present invention can be used for pH detections, wherein it is pH test paper to examine body 8.PH test paper is to refer to indicate sample by color
A kind of test paper of product pH value, such as the examination containing acid-base indicators such as bromothymol blues (Bromothymol Blue, BTB)
Paper, when sample is alkalinity, test paper is in blue-green, is taken on a red color when acid.BTB indicator can be additionally used in the quantitative detection of determinand.
The present invention can be used for urinalysis, wherein it is a kind of urinalysis test paper to examine body 8.Urinalysis test paper usually wraps
Containing a variety of detection reagents, so as to be detected to many kinds of substance.The substance that can be detected mainly include but not limited to leucocyte,
Nitrite, urobilinogen, protein, pH, blood, proportion, ascorbic acid, ketoboidies, bilirubin, glucose sugar.
The present invention can be used for enzyme linked immunosorbent detection.Suction nozzle 2 of the present invention can successively acquire sample, reaction examination respectively
Agent.Examine the antibody (antigen) that fixation can be specifically bound with determined antigen (antibody) in body 8.Test sample is waited for using suction nozzle acquisition
Product make sample infiltration examine body, then determined antigen (antibody) and antibody (antigen) specific bond in inspection body, after reaction balance
Sample to be tested is discharged.Then, with the antibody (antigen) of suction nozzle acquisition enzyme label, i.e. marker, marker is discharged after the completion of reaction
Solution.Finally, zymolyte, i.e. color developing agent are acquired with suction nozzle, makes the marker on detection body that catalyzing hydrolysis or oxidation occur
Reduction reaction and specific color is presented.
The present invention can be used for the quantitative detection of the substances such as phenols in environment measuring, dissolved oxygen, fluoride, cyanide：
When reaction reagent 10 includes 4-AA, body 8 is examined to can be used for measuring the concentration of phenol；
When reaction reagent 10 includes at least one of methylene blue, indigo reagent, body 8 is examined to can be used for environment measuring
The dissolved oxygen in sample is detected in journey；The chemical reaction of methylene blue and indigo reagent and dissolved oxygen generates coloring matter, surveys
Its fixed absorbance, realizes the quantitative detection to dissolved oxygen；
When reaction reagent 10 includes lanthanum nitrate, body 8 is examined to can be used for the detection of the fluoride in environment measuring；Lanthanum nitrate with
Fluorine ion reaction generates blue ternary complexes, is measured to fluoride by spectrophotometry；
When reaction reagent 10 includes sodium alizarine sulphonate and zirconates generation red complex, body 8 is examined also to can be used for environment inspection
The detection of fluoride in survey；When having fluorine ion in sample, zirconium ion in complex compound can be captured, generate colourless zirconium fluoride from
Son releases the sodium alizarine sulphonate of yellow, using ocular estimate, is taken off to the coloration of yellow and standard colorimetric by red according to solution,
To carry out the detection of fluoride and quantify；
Reaction reagent 10 includes pyridine-pyrazol quinoline ketone, and body 8 is examined to pass through for the detection of the cyanide in environment measuring
Spectrophotometry carries out quantitative detection to cyanide.
The present invention can be used for the detection of organophosphor or carbamates in pesticide residue, and wherein reaction reagent 10 includes indigo
Phenol and indophenols acetic acid esters.Organophosphor or carbamates, which have the activity of acetylcholine esterase, in pesticide residue inhibits to make
With since acetylcholine esterase participates in the balanced reaction of the indophenols and the indophenols acetic acid esters to take on a red color in blue, with indophenols and indigo
Phenol acetic acid esters is the activity that indicator can measure acetylcholine esterase, to be detected to pesticide residue.
The present invention can be used for free chlorine detection, and wherein reaction reagent 10 includes N, N- diethyl-Isosorbide-5-Nitrae-phenylenediamine (DPD).
After free chlorine in sample is reacted with DPD, absorbance of the free chlorine at 515nm can be surveyed, to reach in quantitative detection sample
The purpose of chlorine residue.
The present invention can be used for surfactant detection, and wherein reaction reagent 10 includes methylenum careuleum.It is lived using anionic surface
Property agent and methylenum careuleum form blue-colored ionic associated matter, blue-colored ionic associated matter measures it after chloroform extracts under 625nm light sources
Absorbance, and then calculate the concentration of anion surfactant.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (10)
1. a kind of portable detector, which is characterized in that including a liquid-transfering device, a suction nozzle；
There is a cavity for accommodating sample in the suction nozzle；
It is fixed with inspection body in the cavity, the optics of body or sample letter is examined when examining body to be mixed with sample and reacting
Breath changes；
The situation of change for mixing front and back optical information with sample by inspection body, qualitative and quantitative detection is carried out to sample composition；
The liquid-transfering device is equipped with the suction nozzle mounting portion for installing the suction nozzle, and the suction nozzle mounting portion can with the suction nozzle
Dismantling connection；
The both ends of the suction nozzle are equipped with opening, and one end open is the liquid sucting port for importing sample, and another end opening is to use
In the installing port of connection suction nozzle mounting portion, the liquid sucting port is connected with the cavity with the installing port；
Liquid sucting port is located at the lower end of suction nozzle, and installing port is located at the upper end of suction nozzle；
The liquid sucting port of the suction nozzle is micromillimetre size, narrow upper width under suction nozzle internal diameter；
The circular in cross-section or polygon of the suction nozzle；
By the liquid-transfering device, sample collection to suction nozzle internal cavities or is discharged from.
2. a kind of portable detector according to claim 1, it is characterised in that：The suction nozzle is made of transparent material
Suction nozzle.
3. a kind of portable detector according to claim 1, it is characterised in that：The inspection body is one containing reaction examination
The test paper of agent；
The reaction reagent is the reaction reagent for metachromasia to occur with sample；
The inspection body includes pH test paper, urinalysis test paper, enzyme linked immunoassay test paper, ambient water or gas analysis examination
Paper, pesticide test paper, quantitatively at least one of survey chlorine test paper, surfactant residual test paper.
4. a kind of portable detector according to claim 1, it is characterised in that：The inspection body be it is planar, it is described
Examine the central axis one non-90 degree angle of composition of the plane and suction nozzle where body.
5. a kind of portable detector according to claim 1, it is characterised in that：The inspection body be include reaction reagent
Sphere, cylinder or polyhedron；
The reaction reagent is the reaction reagent for metachromasia to occur with sample；
It is fixed in the suction nozzle and examines body frame；
The inspection body frame can fixed inspection body, while ensureing inside suction nozzle to be connected to from liquid sucting port to installing port；
The inspection body is provided with one or more detection zones；
The inspection body contains different reaction reagents in different detection zones；
From different substances metachromasia can occur for the different reaction reagent；
The inspection body is provided with color reference area.
6. a kind of portable detector according to claim 5, it is characterised in that：The inspection body frame has polygonal prism
Shape；
One or more column-like paths are additionally provided with inside the inspection body frame, are used for fixed inspection body；
The inspection body frame is additionally provided with cylindrical recesses, is used for the reference area of fixed inspection body.
7. a kind of portable detector according to claim 1, it is characterised in that：The liquid-transfering device includes one for controlling
Sample preparation product suck or are discharged the syringe of the suction nozzle, are equipped with a hollow chamber in the syringe, in the hollow chamber
Equipped with a moveable piston, the movement of the piston is used for the sucking for sample or discharge drainage；
The side of the piston is fixed with a drive shaft, and the drive shaft can drive piston to be slided in hollow chamber；
Side of the syringe far from the drive shaft is equipped with the communicating passage be connected with the cavity of suction nozzle；
The communicating passage is connected with the chamber far from the drive shaft side in syringe；
The liquid-transfering device further includes a shell, and the syringe is fixed in the shell, and offered on the shell with
The opening of the communicating passage mutual conduction, the opening are located at the suction nozzle mounting portion；
The shell is stretched out in side of the drive shaft far from the communicating passage.
8. a kind of portable detector according to claim 7, it is characterised in that：The shell is fixed with a light source, one
Optical sensor, a measure loop, an indication mechanism and operation button；
The light emission direction of the light source is towards the cavity of the suction nozzle, and the induction direction of the optical sensor is also towards the suction
The cavity of mouth；
Two light-transmitting openings are further opened on the shell, two light-transmitting openings are respectively used to export and the light introduction optical of light source
Sensor；
The light source is fixed on the suction nozzle mounting portion with the optical sensor；
The measure loop is fixed in the shell, for collecting and handling the information from optical sensor；
The indication mechanism is used to show that the handling result of measure loop, the indication mechanism to be fixed on housing exterior walls.
9. a kind of portable detector according to claim 7, it is characterised in that：The shell is fixed with coloured image and adopts
Acquisition means, a measure loop, an indication mechanism and operation button；
The Color Image Acquisition device is fixed on the suction nozzle mounting portion；
The measure loop is fixed in the shell, for collecting and handling the information from Color Image Acquisition device；
The indication mechanism is used to show that the handling result of measure loop, the indication mechanism to be fixed on housing exterior walls.
10. according to a kind of portable detector described in claim 1,7,8 or 9, it is characterised in that：At a mobile terminal
Manage program；A radio transmitting device is fixed in the shell；
The mobile terminal processing routine is installed on any one in mobile phone, wearable device and computer terminal；
The mobile terminal processing routine can realize that human-computer interaction, image procossing, data are shown and data storage；
The radio transmitting device sends it to mobile phone, wearable sets for collecting the information from measure loop
In standby and computer terminal.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710952324.9A CN107796808A (en) | 2017-10-13 | 2017-10-13 | A kind of portable detector |
| CN2017109523249 | 2017-10-13 |
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| Publication Number | Publication Date |
|---|---|
| CN108508006A true CN108508006A (en) | 2018-09-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710952324.9A Pending CN107796808A (en) | 2017-10-13 | 2017-10-13 | A kind of portable detector |
| CN201810243455.4A Pending CN108508006A (en) | 2017-10-13 | 2018-03-23 | A kind of portable detector |
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| CN110389057A (en) * | 2018-04-23 | 2019-10-29 | 上海伯顿医疗设备有限公司 | Integrated multi-functional biological sample processor |
| CN108956592A (en) * | 2018-06-01 | 2018-12-07 | 南京新循环保科技有限公司 | Chemical component detection device |
| CN108760731A (en) * | 2018-06-01 | 2018-11-06 | 南京新循环保科技有限公司 | Chemical composition rapid detection method |
| CN108872560A (en) * | 2018-08-14 | 2018-11-23 | 湖北伽诺美生物科技有限公司 | A kind of portable urine quantitative testing device |
| CN110095456B (en) * | 2019-04-12 | 2021-08-20 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Tobacco virus detection and development device and use method |
| CN111323413B (en) * | 2020-02-17 | 2021-01-15 | 中国农业大学 | Device for detecting pesticide by evaporating solution-nucleic acid test paper |
| CN111879768B (en) * | 2020-07-30 | 2022-03-18 | 上海化工研究院有限公司 | Multifunctional acid-base gradient rapid detection bottle with segmented color development function and application thereof |
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