CN216387074U

CN216387074U - Immunodifiltration detection kit

Info

Publication number: CN216387074U
Application number: CN202122430097.8U
Authority: CN
Inventors: 高柯轩; 徐志华; 方阿社; 崔磊; 韩颖黎; 牛星超; 杨振; 赵琪
Original assignee: Shihezi University
Current assignee: Shihezi University
Priority date: 2021-10-09
Filing date: 2021-10-09
Publication date: 2022-04-26
Anticipated expiration: 2031-10-09

Abstract

The utility model discloses an immune infiltration detection kit, which comprises a protection box, an infiltration detection card and a polystyrene microsphere cutting sleeve, wherein the infiltration detection card and the polystyrene microsphere cutting sleeve are both arranged in the protection box, and the top end of the protection box is provided with a box cover; the utility model can quickly and accurately detect the result by matching the percolation detection card and the polystyrene microsphere cutting sleeve, improves the detection efficiency, is convenient for bedside diagnosis, is simple and suitable for household self-detection, displays the detection result in three different colors, is easy to read and distinguish, only has positive reaction on various human or animal serum samples of brucella, and has negative result on serum samples infected by other pathogens, can carry out typing detection on brucella of sheep and cattle in the serum samples, and has the advantages of strong sensitivity, high sensitivity and long quality guarantee period.

Description

Immunodifiltration detection kit

Technical Field

The utility model relates to the technical field of immunological diagnosis and detection, in particular to an immunodiafiltration detection kit.

Background

Brucellosis, also known as brucellosis, brucellosis and wavy fever, is a zoonosis infectious disease caused by brucellosis infection, has no typical symptoms, has low content of pathogenic bacteria in blood, often cannot be monitored in clinical diagnosis, shows false negative results, is interfered and limited by a plurality of conditions when timely and accurate diagnosis is needed in serology, is particularly difficult to correctly diagnose, is easy to be confused with clinical characteristics of syphilis and tuberculosis, and has clinically variable symptoms at the beginning of disease, so that research and innovation on the clinical diagnosis technology of brucellosis are very important for controlling the epidemic of brucellosis.

In the animal husbandry, the brucellosis causes orchitis, abortion, nonpregnant and other symptoms of animals, so that young animals can grow up to 100 to 140 thousands of animals, the economic loss is about 2 hundred million yuan, and the disease seriously threatens the public health safety and the continuous development of the animal husbandry.

The serological examination method of brucellosis comprises a test tube agglutination test (SAT), a Complement Fixation Test (CFT), a tiger red plate agglutination test (RBPT), an ELISA brucellosis detection technology, a bacteria isolation culture technology, a PCR detection technology and the like, can detect the brucellosis, but professional technicians and instruments for molecular biological detection are lacked in basic diagnosis and treatment institutions and animal veterinary stations, and the detection modes have the defects of long detection period, complicated detection steps, high cost and the like, and are difficult to popularize and use in minority regions of frontier regions, particularly high-incidence pastoral areas of the brucellosis, so the immune infiltration detection kit provided by the utility model is used for solving the problems in the prior art.

SUMMERY OF THE UTILITY MODEL

In view of the above problems, the present invention provides an immuno-filtration assay kit, which can rapidly and accurately detect a result by using a filtration assay card and a polystyrene microsphere card sleeve in combination, and has the advantages of high sensitivity, high sensitivity and long shelf life, and the detection result is displayed in three different colors, is easy to read and distinguish, can be used for typing detection of brucella melitensis and brucella bovis in a serum sample, and is convenient for bedside diagnosis, simple and suitable for home self-test.

In order to achieve the purpose of the utility model, the utility model is realized by the following technical scheme: an immune infiltration detection kit comprises a protection box, an infiltration detection card and a polystyrene microsphere cutting sleeve, wherein the infiltration detection card and the polystyrene microsphere cutting sleeve are arranged in the protection box, a box cover is arranged at the top end of the protection box, the infiltration detection card comprises a detection card upper cover, a polystyrene microsphere cushion and a detection card outer sleeve, the detection card upper cover is arranged at the top end of the detection card outer sleeve, the polystyrene microsphere cushion is arranged between the detection card upper cover and the detection card outer sleeve, a detection card visible window is arranged on the detection card upper cover, the polystyrene microsphere cutting sleeve comprises a cutting sleeve upper cover body, a nitrocellulose membrane, absorbent paper and a cutting sleeve lower cover body, the cutting sleeve upper cover body is embedded and arranged at the top end of the cutting sleeve lower cover body, the nitrocellulose membrane and the absorbent paper are arranged between the cutting sleeve upper cover body and the cutting sleeve lower cover body, and the nitrocellulose membrane is positioned at the top end of the absorbent paper, the upper cover body of the clamping sleeve is provided with a visual clamping sleeve window.

The further improvement lies in that: the bottom end of the detection card outer sleeve is provided with a cylinder matched with the visible window of the card sleeve, the detection card outer sleeve is of a hollow design communicated up and down, the top end of the detection card outer sleeve is provided with a first groove matched with the polystyrene microsphere pad, and the bottom end of the inner side of the lower cover body of the card sleeve is provided with a second groove matched with the absorbent paper.

The further improvement lies in that: the detection card visual window and the polystyrene microsphere pad are both in circular design, the diameter of the detection card visual window is smaller than that of the polystyrene microsphere pad, the card sleeve visual window and the nitrocellulose membrane are both in circular design, and the diameter of the card sleeve visual window is smaller than that of the nitrocellulose membrane.

The further improvement lies in that: the top and the bottom of protection box all are fixed with the gomphosis mounting bar, the gomphosis mounting groove that matches with the gomphosis mounting bar is seted up to the lid bottom.

The further improvement lies in that: the protection box is equipped with the sponge fixed bed, detection card standing groove and cutting ferrule standing groove have been seted up on the sponge fixed bed, be equipped with the test tube groove between detection card standing groove and the cutting ferrule standing groove, one side that the cutting ferrule standing groove was kept away from to the detection card standing groove is equipped with the dropper groove, one side that the detection card standing groove was kept away from to the cutting ferrule standing groove is equipped with buffer solution bottle groove.

The further improvement lies in that: the inboard bottom equidistance of protection box is fixed with damping spring, the damping spring top is fixed with the shock attenuation board, the inboard top of lid is fixed with the rubber buffer board.

The utility model has the beneficial effects that: the utility model comprises a protection box, an infiltration detection card and a polystyrene microsphere cutting sleeve, and the infiltration detection card and the polystyrene microsphere cutting sleeve are matched for use, so that the result can be quickly and accurately detected, the detection efficiency is improved, bedside diagnosis is convenient and simple, the kit is suitable for household self-detection, the detection result is displayed in three different colors, the reading and distinguishing are easy, the positive reaction is only performed on the serum samples of various human beings or animals with brucella, the negative result is performed on the serum samples infected by other pathogens, the typing detection of the brucella infection of sheep species and cattle species can be performed on the antibody in the serum sample, and the kit has the advantages of strong sensitivity, high sensitivity and long quality guarantee period.

Drawings

Fig. 1 is a front view of a protection case and a case cover according to a first embodiment of the present invention;

fig. 2 is a front sectional view of a protection case and a case cover according to a first embodiment of the present invention;

FIG. 3 is a top view of a protective case according to a first embodiment of the present invention;

FIG. 4 is a perspective view of a polystyrene microsphere ferrule according to a first embodiment of the present invention;

FIG. 5 is an exploded view of a polystyrene microsphere ferrule according to a first embodiment of the present invention;

FIG. 6 is a perspective view of a diafiltration detection card according to a first embodiment of the utility model;

FIG. 7 is an exploded view of a percolation test card in accordance with a first embodiment of the utility model;

fig. 8 is a top view of the case cover according to the second embodiment of the present invention.

Wherein: 1. a protection box; 2. a percolation test card; 3. a polystyrene microsphere cutting sleeve; 4. a box cover; 5. detecting an upper cover of the card; 6. a polystyrene microsphere pad; 7. detecting an outer sleeve of the card; 8. detecting a card visible window; 9. the cutting sleeve is provided with a cover body; 10. a nitrocellulose membrane; 11. absorbent paper; 12. a ferrule lower cover body; 13. a visible window of the card sleeve; 14. a cylinder; 15. a first groove; 16. a second groove; 17. embedding the mounting bar; 18. embedding the mounting groove; 19. a sponge fixing layer; 20. detecting a card placing groove; 21. a card sleeve placing groove; 22. a damping spring; 23. a damper plate; 24. a rubber buffer plate; 25. a rubber pad; 26. and (4) anti-skid lines.

Detailed Description

In order to further understand the present invention, the following detailed description will be made with reference to the following examples, which are only used for explaining the present invention and are not to be construed as limiting the scope of the present invention.

Example one

According to the drawings of fig. 1, 2, 3, 4, 5, 6 and 7, the embodiment provides an immunofiltration detection kit, which comprises a protection box 1, an infiltration detection card 2 and a polystyrene microsphere cutting sleeve 3, wherein the polystyrene microsphere cutting sleeve 2 is characterized in that the cutting sleeve is formed by mixing three kinds of colored polystyrene microspheres in equal proportion, wherein, the red polystyrene microsphere and the blue polystyrene microsphere are marked and coupled with staphylococcus aureus protein A, the purple polystyrene microsphere is marked and coupled with streptavidin, the three kinds of colored polystyrene microsphere-protein conjugates are mixed in equal proportion and sprayed on the cutting sleeve, the infiltration detection card 2 and the polystyrene microsphere cutting sleeve 3 are both arranged in the protection box 1, the top end of the protection box 1 is provided with a box cover 4, the infiltration detection card 2 comprises a detection card upper cover 5, a polystyrene microsphere pad 6 and a detection card outer sleeve 7, the detection card upper cover 5 is arranged at the top end of the detection card outer sleeve 7, the polystyrene microsphere cushion 6 is arranged between the upper detection card cover 5 and the outer detection card sleeve 7, the upper detection card cover 5 is provided with a visible detection card window 8, the polystyrene microsphere cutting sleeve 3 comprises an upper cutting sleeve cover 9, a nitrocellulose membrane 10, absorbent paper 11 and a lower cutting sleeve cover 12, the upper cutting sleeve cover 9 is embedded and installed at the top end of the lower cutting sleeve cover 12, the nitrocellulose membrane 10 and the absorbent paper 11 are both arranged between the upper cutting sleeve cover 9 and the lower cutting sleeve cover 12, the nitrocellulose membrane 10 is positioned at the top end of the absorbent paper 11, the upper cutting sleeve cover 9 is provided with a cutting sleeve visible window 13, the nitrocellulose membrane 10 is a reaction membrane, the nitrocellulose membrane 10 is coated with two detection zones and a quality control zone, the sequence is a first detection zone, a second detection zone and a quality control zone, the interval between every two detection zones is 0.3cm, and the common antigen BP26 of the Brucella bovis and Brucella melitensis is sequentially used as a first detection zone, bovine brucella and ovine brucella typing detection antigen OMP31 is used as a second detection area, BSA biotin is used as a quality control area, and membrane protein OMP31 is not expressed in the bovine brucella, so that only one detection area is colored and is red, the detection area is positive serum infected with the bovine brucella, the two detection areas are colored, and the detection area is red and blue, and the detection area is positive serum infected with the ovine brucella.

The bottom end of the detection card outer sleeve 7 is provided with a cylinder 14 matched with the card sleeve visual window 13, the detection card outer sleeve 7 is of a hollow design communicated up and down, the top end of the detection card outer sleeve 7 is provided with a first groove 15 matched with the polystyrene microsphere pad 6, and the bottom end of the inner side of the card sleeve lower cover body 12 is provided with a second groove 16 matched with the absorbent paper 11.

The detection card visual window 8 and the polystyrene microsphere pad 6 are both in circular design, the diameter of the detection card visual window 8 is smaller than that of the polystyrene microsphere pad 6, the cutting sleeve visual window 13 and the nitrocellulose membrane 10 are both in circular design, and the diameter of the cutting sleeve visual window 13 is smaller than that of the nitrocellulose membrane 10.

The top and the bottom of protection box 1 all are fixed with the gomphosis mounting bar 17, and the gomphosis mounting groove 18 that matches with gomphosis mounting bar 17 is seted up to lid 4 bottom, matches through the gomphosis of gomphosis mounting bar 17 and gomphosis mounting groove 18 to make lid 4 be convenient for install the top and the bottom at protection box 1.

Protection box 1 is equipped with sponge fixed bed 19, detection card standing groove 20 and cutting ferrule standing groove 21 have been seted up on the sponge fixed bed 19, be equipped with the test tube groove between detection card standing groove 20 and the cutting ferrule standing groove 21, one side that cutting ferrule standing groove 21 was kept away from to detection card standing groove 20 is equipped with the drip tube groove, one side that detection card standing groove 20 was kept away from to cutting ferrule standing groove 21 is equipped with buffer solution bottle groove, place infiltration detection card 2 and polystyrene microballon cutting ferrule 3 through sponge fixed bed 19, play the guard action when playing the fixed action.

The inboard bottom equidistance of protection box 1 is fixed with damping spring 22, and damping spring 22 top is fixed with damping plate 23, and the inboard top of lid 4 is fixed with rubber buffer board 24, uses through damping plate 23 and the cooperation of rubber buffer board 24, protects infiltration detection card 2 and polystyrene microballon cutting ferrule 3's bottom and top respectively to avoid it to receive external force striking easily and take place to damage.

Example two

According to the figure 8, the top end of the box cover 4 is fixed with the rubber pads 25 which are distributed at equal intervals, the top ends of the rubber pads 25 are printed with the anti-skid lines 26, the box cover 4 is inverted and the bottom end of the protection box 1 is fixed at the bottom end of the protection box 1 under the condition that the embedded mounting strips 17 at the bottom end of the protection box 1 are matched with the embedded mounting grooves 18, so that the protection box 1 is supported while the space is saved, and the anti-skid function is realized on the protection box 1 under the action of the rubber pads 25 and the anti-skid lines 26.

When the kit is used, when a detection sample is a serum sample or a tissue fluid sample, the detection sample is sucked by a straw and directly dripped into the card sleeve visual window 13 of the polystyrene microsphere card sleeve 3, and color development can be carried out when the liquid completely permeates; when the detected sample is a whole blood sample, mixing the whole blood sample with the comprehensive buffer solution according to the volume ratio of 1:3, namely sucking 1 drop of whole blood by using a suction pipe, mixing the whole blood with 3 drops of the comprehensive buffer solution, pouring the mixture into a visible window 8 of a detection card of the percolation detection card 2, and developing when the liquid is completely infiltrated; the third color development step is specifically as follows: placing the percolation detection card 2 in the polystyrene microsphere cutting sleeve 3, and dripping 5-8 drops of comprehensive buffer solution into the percolation detection card 2; and (4) observing a detection result: removing the percolation detection card 2, and reading the result within 10 min; the nitrocellulose membrane 10 is positive serum infected with Brucella melitensis when only one detection area is red except the quality control area is purple; the nitrocellulose membrane 10 is positive serum infected with Brucella melitensis when the other two detection areas respectively show red and blue except the quality control area shows purple; only when the quality control area is purple and the detection area is not colored, the serum is negative serum which is not infected by brucella; if no purple color appears on the quality control area, the percolation test card 2 is invalid regardless of whether the detection points are colored or not.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides an immunofiltration assay kit, includes protection box (1), filtration detection card (2) and polystyrene microballon cutting ferrule (3), its characterized in that: infiltration detection card (2) and polystyrene microballon cutting ferrule (3) all locate in protection box (1), protection box (1) top is equipped with lid (4), infiltration detection card (2) are including detecting card upper cover (5), polystyrene microballon pad (6) and detection card outer sleeve (7), detection card upper cover (5) are located and are detected card outer sleeve (7) top, polystyrene microballon pad (6) are located and are detected between card upper cover (5) and detection card outer sleeve (7), it can window (8) to have seted up the detection card on detection card upper cover (5), polystyrene microballon cutting ferrule (3) are including cutting ferrule upper cover body (9), nitrocellulose membrane (10), absorbent paper (11) and cutting ferrule lower cover body (12), cutting ferrule upper cover body (9) gomphosis is installed in lower cover body (12) top, nitrocellulose membrane (10) and absorbent paper (11) are all located cutting ferrule upper cover (9) and cutting ferrule lower cutting ferrule lid body (12) top 12) And the nitrocellulose membrane (10) is positioned at the top end of the absorbent paper (11), and a sleeve cover body (9) on the sleeve cover is provided with a sleeve cover visual window (13).

2. The immunodiafiltration detection kit according to claim 1, wherein: the detection card is characterized in that a cylinder (14) matched with a card sleeve visual window (13) is arranged at the bottom end of the detection card outer sleeve (7), the detection card outer sleeve (7) is of a hollow design communicated from top to bottom, a first groove (15) matched with the polystyrene microsphere pad (6) is formed in the top end of the detection card outer sleeve, and a second groove (16) matched with the absorbent paper (11) is formed in the bottom end of the inner side of the card sleeve lower cover body (12).

3. The immunodiafiltration detection kit according to claim 1, wherein: the detection card visual window (8) and the polystyrene microsphere pad (6) are both in a circular design, the diameter of the detection card visual window (8) is smaller than that of the polystyrene microsphere pad (6), the card sleeve visual window (13) and the nitrocellulose membrane (10) are both in a circular design, and the diameter of the card sleeve visual window (13) is smaller than that of the nitrocellulose membrane (10).

4. The immunodiafiltration detection kit according to claim 1, wherein: the top and the bottom of protection box (1) all are fixed with gomphosis mounting bar (17), gomphosis mounting groove (18) that match with gomphosis mounting bar (17) are seted up to lid (4) bottom.

5. The immunodiafiltration detection kit according to claim 1, wherein: protection box (1) is equipped with sponge fixed layer (19), detection card standing groove (20) and cutting ferrule standing groove (21) have been seted up on sponge fixed layer (19), be equipped with the test tube groove between detection card standing groove (20) and cutting ferrule standing groove (21), one side that cutting ferrule standing groove (21) were kept away from in detection card standing groove (20) is equipped with the dropper groove, one side that detection card standing groove (20) were kept away from in cutting ferrule standing groove (21) is equipped with buffer solution bottle groove.

6. The immunodiafiltration detection kit according to claim 1, wherein: protection box (1) inboard bottom equidistance is fixed with damping spring (22), damping spring (22) top is fixed with damping plate (23), lid (4) inboard top is fixed with rubber buffer board (24).