CN110387322A - Automatic nucleic acid extracts detection system - Google Patents
Automatic nucleic acid extracts detection system Download PDFInfo
- Publication number
- CN110387322A CN110387322A CN201810312677.7A CN201810312677A CN110387322A CN 110387322 A CN110387322 A CN 110387322A CN 201810312677 A CN201810312677 A CN 201810312677A CN 110387322 A CN110387322 A CN 110387322A
- Authority
- CN
- China
- Prior art keywords
- tested
- sample
- pipette tips
- nucleic acid
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A kind of automatic nucleic acid extraction detection system, including nucleic acid draw-out device, Wiping mechanism and optical detection apparatus, the injection ram with pipette tips is moved to above the test tube to be measured on rack for test tube to be measured using the driving platform of nucleic acid draw-out device, injection ram device is drawn in test tube to be measured, purified sample to be tested, and it is placed in pipette tips, in the tested area of sample that the injection ram for having drawn purified sample to be tested is moved to optical detection apparatus by driving platform, injection ram drips the purified sample to be tested in pipette tips in the tested area of sample, purified sample to be tested is detected through optical detection apparatus, to obtain the detection data of purified sample to be tested, recycle the tested area of cleansing tissue wipe samples, whereby, other than it can automatically complete the detection of purified sample to be tested, manpower can also be saved and carry out detection point After analysis and avoid the problem that sample to be tested cross contamination when detecting.
Description
Technical field
The present invention is to be related to a kind of nucleic acid to extract detection device, in particular to integrate nucleic acid extraction device, optics is examined
The automatic nucleic acid for surveying device and wiping arrangement extracts detection system.
Background technique
Foranalysis of nucleic acids is the analysis method that biomedical research now and clinical diagnosis are often used, therefore nucleic acid is separated
It is people in the industry in the direction for making great efforts development that how nucleic acid extraction, which more effectively considers or obtain the nucleic acid of high-purity,.
Currently used nucleic acid extraction mode is divided into two major classes, and one kind is organic solvent abstraction purification method, with lysate such as phenol-
Chloroform is separated by cell dissolution (lysis), using ultrahigh speed from factor solutions, and DNA can be in aqueous layer and its at this time
He separates composition (such as protein), finally ethyl alcohol or isopropanol is recycled to precipitate by DNA, the DNA purified.It is another
It is then tubing string abstraction purification method (column purification), cell is mainly broken by it with teepol
Afterwards, through ferment, inhibitor and salt etc., reach removal impurity, so that DNA is saltoutd and be purified into DNA.However traditional DNA
Purification process it is time-consuming, can not also extract completely, the low output of DNA and the organic solvent in extraction process there is mansion erosion poison
Property etc., still there are many problem is to be resolved.
With making rapid progress for science and technology, develop with the purifying concept of Magneto separate (magnetic separation), in conjunction with
The concept of molecular biosciences, physics and chemistry carries out compatibility through combining the surface ligand (ligand) of magnetic nanometer different
Reaction, can be used for the purifying of different material, such as: protein, DNA, antibody etc..In nucleic acid field, poly-A such as
MRNA, DNA purifying and secondary generation NGS have the participation of this technology.Such reversible fixation of way of purification general term solid-liquid
Separating and purifying technology (Solid phase reversible immobilisation, SPRI), compared in the past utilize simple silicon
Particle has better separative efficiency.
The principle of magnetic bead essentially consists in magnetic bead surfaces and covers one layer of silicon that can adsorb nucleic acid, by magnetic bead with to be purified
After specimen mixing, using pH value and the difference of ion concentration, the adsorption capacity of nucleic acid is inhaled in magnetic bead to regulate and control silicon on magnetic bead
After attached nucleic acid, nucleic acid and magnetic bead is allowed to separate to reach the result of purifying through washing buffer again after impurity cleans up.
However, can be purified nucleic acid from a specimen to be detected using magnetic bead, but it is applied to current extraction
Purify for board, for adsorb the magnetic devices of magnetic bead then can only single Zhi Zuoye one by one, but obtained purifying
Solution to be analyzed again, at present can only be in a manual manner by purified sample extraction be gone out in test tube one by one
Come, is then analyzed in micro light splitting luminance meter, to obtain the suction brightness of purified sample and then obtain its concentration, but
It is to extract sample in a manual manner to be easy to produce cross contamination, and make the concentration of purified sample error.If to
Survey a specimen quantity it is quite huge when, in the middle have sample to be tested polluted or data error when, needing will by the gross
Sample detects again, is labor intensive and time.In addition, due to the inaccuracy in data, and cause for being extracted a specimen
The mistaken diagnosis of human body or medical careless mistake, then be not the problem of detection can compensate again.
Summary of the invention
The main purpose of the present invention is to provide a kind of automatic nucleic acid to extract detection system, using nucleic acid draw-out device by
It is extracted in testing tube and is loaded with purified sample to be tested, so that nucleic acid draw-out device has the sample to be tested extracted drop
The sample of the optical detection apparatus of wiping arrangement is detected in tested area, and after to be detected, wiping arrangement fills optical detection
Liquid Residue wiped clean in the tested area of the sample set, therefore, by nucleic acid draw-out device, optical detection apparatus and wiping arrangement with
Automation mode combines, and the data of the sample to be tested can be influenced to avoid artificial caused cross contamination, and complete
After analysis, the pipette tips extracted are placed directly in discarded slot by nucleic acid draw-out device, need not be using manually to nucleic acid pumping
It takes device to be replaced or cleaned, manpower can be saved.
It is another object of the present invention to be moved to below pipette tips disk using pushing unit and pipette tips to be used will be needed to jack up,
Allow injection ram can be easily in conjunction with pipette tips.
According to above-mentioned purpose, the invention discloses a kind of automatic nucleic acid to extract detection system, including nucleic acid draw-out device and light
Learn detection device, amplifying nucleic acid draw-out device at least by pedestal, driving platform, injection ram, be loaded with it is purified to test sample
The test tube to be measured of more of product is arranged on rack for test tube to be measured, more pipette tips and pushing unit are constituted.Platform is driven, on the base
Front and back moves back and forth and moves left and right；More injection rams, each injection ram are arranged in piston platform, each note
Piston is penetrated to move up and down in piston platform with vertical direction；More pipette tips, each pipette tips are arranged on pipette tips disk；And top
Unit is pushed away, is arranged below pipette tips disk, pushing unit has drive motor, drives pushing unit in pipette tips disk by drive motor
Lower section moves back and forth and can position to the pipette tips to be jacked up, by pushing unit by the pipette tips of top by court on pipette tips disk
It is ejected to the direction of injection ram, and allows injection ram in conjunction with pipette tips.
Optical detection apparatus is set to the lower section of driving platform, and optical detection apparatus is at least by the tested area of sample, survey ray machine
Structure, cleansing tissue and control unit are constituted.The tested area of sample, to accommodate purified sample to be tested.Light-measuring mechanism has and surveys
The ontology of radiant end and pivot end, pivot end and optical detection apparatus is pivotally connected, and is surveyed radiant end and is put to emit light source irradiation
The purified sample to be tested in the tested area of sample is set, and obtains the suction brightness of purified sample to be tested and corresponding dense
Degree.Control unit drives platform by the injection with pipette tips whereby to control light-measuring mechanism through radiant transmitting light source is surveyed
Piston is moved on rack for test tube to be measured, one of them for being intended to be detected is loaded with above the test tube to be measured of sample to be tested, injection
Piston draws the purified sample to be tested in test tube to be measured, and purified sample to be tested is placed in pipette tips, drives
The sample that the injection ram with pipette tips for having drawn purified sample to be tested is moved to optical detection apparatus by platform is tested
Qu Shang, for injection ram by the purified sample to be tested drop in pipette tips in the tested area of sample, control unit controls light-measuring mechanism hair
Light source is penetrated to detect the purified sample to be tested in the tested area of sample, and obtains the testing number of purified sample to be tested
According to the cleansing tissue that the control unit control of optical detection apparatus is surveyed on radiant end is drawn purified in the tested area of sample
Sample to be tested, and by the tested area's wiped clean of sample.
According to above-mentioned purpose, the present invention also provides another automatic nucleic acid to extract detection system, comprising nucleic acid draw-out device and
Optical detection apparatus, amplifying nucleic acid draw-out device includes pedestal, reagent strip placing platform, driving platform, more injection rams, more
Root pipette tips and pushing unit.Reagent strip placing platform, to set at least one more reagent strip, and reagent strip has multiple test tubes,
And purified sample to be tested is loaded at least one test tube.Driving platform, front and back moves back and forth on the base and left and right is moved
It is dynamic.More injection rams, each injection ram are arranged in piston platform, and each injection ram is in piston platform to hang down
Histogram moves up and down.More pipette tips, each pipette tips are arranged on pipette tips disk.Pushing unit is arranged below pipette tips disk, top
Unit is pushed away with drive motor, drive motor drives pushing unit to move back and forth below pipette tips disk and can position to being intended to jack up
Pipette tips below, by pushing unit in a manner of moving up and down by the pipette tips of top by pipette tips disk towards the direction of injection ram
Ejection, and allow injection ram in conjunction with pipette tips.
Optical detection apparatus is set to the lower section of driving platform, and optical detection apparatus includes the tested area of sample, to accommodate
Purified sample to be tested, light-measuring mechanism have and survey radiant end and pivot end, the ontology pivot of pivot end and optical detection apparatus
It connects, surveys radiant end to emit light source, cleansing tissue, there are multiple cleaning blocks, and cleansing tissue setting is on surveying radiant end
And control unit, drive platform by the note with pipette tips whereby through radiant end transmitting light source is surveyed to control light-measuring mechanism
It penetrates piston to be moved to above the test tube in reagent strip with purified sample to be tested, driving platform driving has the injection of pipette tips
Piston is towards the examination in-pipe with purified sample to be tested, to draw in the test tube with purified sample to be tested
Purified sample to be tested and be placed in pipette tips, driving platform by drawn purified sample to be tested with pipette tips
Injection ram moves up, so that test tube of the pipette tips far from purified sample to be tested, and will draw purified to test sample
The injection ram with pipette tips of product is moved in the tested area of sample of optical detection apparatus, and injection ram will be pure in pipette tips
The sample to be tested of change drips in the tested area of sample, purified to test sample to detect through control unit control light-measuring mechanism transmitting light source
Product, and the detection data of purified sample to be tested is obtained, the control unit control of optical detection apparatus is on surveying radiant end
Cleansing tissue draw purified sample to be tested in the tested area of sample, and by the tested area's wiped clean of sample.
Detailed description of the invention
Fig. 1 is technology disclosed according to the present invention, indicates automatic and extracts nucleic acid detection system block schematic diagram.
Fig. 2 is the step flow diagram for indicating magnetic bead type nucleic acid extraction.
Fig. 3 is technology disclosed according to the present invention, indicates that standing in rack is arranged in the automatic nucleic acid detection system that extracts
Body figure.
Fig. 4 is technology disclosed according to the present invention, indicates the automatic schematic cross-section for extracting nucleic acid detection system.
Fig. 5 is technology disclosed according to the present invention, indicates automatic and extracts in nucleic acid detection system, nucleic acid draw-out device,
The block schematic diagram of optical detection apparatus and wiping arrangement.
Fig. 6 disclosed technology according to the present invention, indicates the automatic step flow chart for extracting nucleic acid detection system.
Fig. 7 A and Fig. 7 B disclosed technology according to the present invention, indicate the automatic another step stream for extracting nucleic acid detection system
Cheng Tu.
Specific embodiment
In order to make the purpose of the present invention, technical characteristic and advantage, can more correlative technology field personnel understood, and be able to
Implement the present invention, cooperates appended schema herein, specifically illustrates technical characteristic and embodiment of the invention, and enumerate preferable reality
Apply example further explanation.It is not also needed with the schema hereinafter compareed to express signal related with feature of present invention
It is completely drawn according to practical situation.And the technology well-known to those skilled in the art involved in the explanation of this case embodiment
Content is also no longer stated.
Firstly, please referring to Fig. 1.Fig. 1 is to indicate to extract nucleic acid detection system block schematic diagram automatically.It is automatic to take out in Fig. 1
Taking nucleic acid detection system 1 includes reagent strip 2, nucleic acid draw-out device 3 and optical detection apparatus 4, wherein reagent strip 2 and nucleic acid are taken out
Taking device 3 to combine can be as Nucleic acid purification device, and nucleic acid draw-out device 3 and optical detection apparatus 4 are electrically connected.
In another optionally embodiment, automatic extract further comprises external device (ED) 6 in nucleic acid detection system 1, and optical detection
Device 4 and external device (ED) 6 are electrically connected, by external device (ED) 6 by optical detection apparatus 4 detect obtained data output or
Storage.
It is noted that it is (or unknown to detect sample to be tested using automatic extraction nucleic acid detection system 1 of the invention
A nucleic acid specimen for concentration), first a specimen to be measured to be purified, purification step is using known common magnetic bead type nucleic acid
Extracting process, used nucleic acid purification separator, which can be to utilize, is arranged in automatic extraction nucleic acid detection system of the invention
Reagent strip 2 and nucleic acid draw-out device 3 in 1 are integrated and are become except Nucleic acid purification device, selective can also be not required to
Purifies and separates step is done using automatic extraction nucleic acid detection system 1 of the invention.
However, being the advantages of carrying out purification step using automatic extraction nucleic acid detection system 1 of the invention, it is only necessary to
Automatic extract increases the reagent strip placing platform for placing reagent strip 2 (not Fig. 1 shows) in nucleic acid detection system 1, and purifies point
All be from device used in other and nucleic acid draw-out device it is identical, only operating procedure is different, for the user may be used
To complete in same system, can not need that an expense is spent to buy expensive and function again for the user
The similar equipment of energy can also save the working space that entire automatic nucleic acid extracts.
The method for purifying and separating steps flow chart of a nucleic acid specimen is sketched as shown in Fig. 2, simultaneously while together referring to Fig. 3.Firstly, step
Rapid 20, lysate is put into the reagent strip with multiple test tubes for being loaded with specimen solution and suitable magnetic bead to be purified
In, so that specimen solution to be purified reacts to form mixed liquor with lysate, and can be adsorbed in mixed liquor using magnetic bead
Nucleic acid.In step 20, liquid solution to be checked to be purified is reacted via with lysate, so that lysate can break inspection to be purified
The cell of liquid solution, allows nucleic acid to release in mixed liquor, and the nucleic acid released in mixed liquor is adsorbed by magnetic bead.It connects
, step 22, test tube tube wall is leaned against to adsorb the magnetic bead for having adsorbed nucleic acid in test tube using magnet.Step 24, it removes and is trying
Mixed liquor in pipe.In step 24, since the magnetic bead for having adsorbed nucleic acid has been adsorbed on the tube wall of test tube by magnet,
It is free from the solution of nucleic acid in remaining mixed liquor, therefore is drawn using transfer pipet(te) and does not contain the molten of nucleic acid in test tube
Liquid, reservation is adsorbed by magnet, and has adsorbed the magnetic bead of nucleic acid.Step 26, magnetic bead is with nucleic acid separating step to have been purified
A nucleic acid specimen.Here, a purified nucleic acid specimen is in the present invention as purified in automatic nucleic acid extraction system
Sample to be tested.
In step 26, first magnet is removed, so that the magnetic bead for being adsorbed with nucleic acid loses the attraction of magnet and falls in examination
In pipe, cleaning solution is added in test tube at this time, has adsorbed the magnetic bead of nucleic acid to infiltrate and rinse, the purpose that cleaning solution is added is
In order to rinse the other impurities on magnetic bead.Then, then by magnet close to test tube, the magnetic bead of nucleic acid will be adsorbed with again
It is attracted on test tube tube wall.Then, transfer pipet(te) is recycled to remove invisible spectro cleaning solution, likewise, in vitro only
Reservation is adsorbed by magnet, and has adsorbed the magnetic bead of nucleic acid.Then it, then by eluent is added in test tube, is rushed by eluent
The nucleic acid on magnetic bead is mentioned, allows nucleic acid and Beads enrichment, and is dissolved in eluent.Next, recycle magnet close to test tube tube wall,
By magnetic bead being adsorbed on test tube tube wall by magnet again.There is no any nucleic acid of absorption on magnetic bead at this time.Finally, sharp
It with transfer pipet(te), by the eluent with nucleic acid by being taken out in test tube, and is seated in save and be saved in test tube, and complete
The purification step of specimen solution.And it can be with demand with different conditions via purified sample to be tested obtained after purifying
To save.
It is noted that above-mentioned steps 20 to step 26 can use disclosed herein automatic extraction detection of nucleic acids system
The piston platform of the driving platform 32 in nucleic acid draw-out device 3, storing injection ram (injection piston) 342 in system
34, it puts the pipette tips disk 36 of pipette tips 362 and the pushing unit 38 with motor walks to complete the purifying of above-mentioned specimen solution
Suddenly, and specimen solution after completing purifying, can be such as abovementioned steps 26, using transfer pipet(te), by the eluent with nucleic acid
By being taken out in test tube, and it is seated in the test tube to be measured of rack for test tube to be measured and is saved.Or be according to it is purified to
The characteristic for surveying a specimen, must detect immediately as the specimen to be measured can not save for a long time, then can directly utilize core of the invention
Sour draw-out device 3 directly extracts a purified specimen to be measured, and is detected and be somebody's turn to do via optical detection apparatus 4
The data of a specimen to be measured finally recycle cleansing tissue 50 to draw the specimen to be measured on optical detection apparatus 4, disposable complete
At the wiped clean of the purifying of nucleic acid, the extraction of nucleic acid, the detection of nucleic acid and sample.In addition, in the purifying of above-mentioned meaning
A specimen to be measured and purified sample to be tested be identical component, only a purified specimen to be measured by purification step it
Afterwards, the step of directly being extracted and detected, and purified sample to be tested is then to be saved a purified specimen to be measured
It is extracted and is detected again later, be intended merely to be relatively easy to illustrate and distinguished in statement herein, but it is purified
A specimen to be measured and purified sample to be tested are identical component.Therefore, followed by in the present invention there is nucleic acid to take out below
The automatic extraction nucleic acid detection system 1 of device 3 and optical detection apparatus 4 is taken to be further described.
It please also refer to Fig. 3, Fig. 4 and Fig. 5.Fig. 3 indicates that the automatic perspective view for extracting nucleic acid detection system, Fig. 4 indicate certainly
The dynamic schematic cross-section for extracting nucleic acid detection system and Fig. 5 indicate automatic and extract in nucleic acid detection system, nucleic acid draw-out device and
The block schematic diagram of optical detection apparatus.As shown in figure 3, extracting the nucleic acid draw-out device 3 and optics of nucleic acid detection system 1 automatically
Detection device 4 is to be incorporated into a rack 11, and pedestal 31 is provided in rack 11, wherein the base of nucleic acid draw-out device 3
Seat 31 is pivotally connected with rack 11, so that having accommodating empty between nucleic acid draw-out device 3 and the bottom (not shown in the figure) of rack 11
Between 310, and optical detection apparatus 4 is then arranged in the accommodating space 310 between nucleic acid draw-out device 3 and the bottom of rack 11,
Therefore, in the present invention, cleansing tissue 50 (as shown in Figure 5) is incorporated on optical detection apparatus 4, so that being detected in sample to be tested
Later, cleansing tissue 50 directly can automatically wipe optical detection apparatus 4, therefore not need to examine as in the prior art in optics
Device 4 is surveyed after sample to be tested has detected, it is also necessary to lean on manpower to wipe removing sample to be tested, to carry out inspection next time
It surveys.Therefore, the cleansing tissue 50 of the prior art manually used is incorporated on optical detection apparatus 4 and is miniaturized, and
The automatic extraction nucleic acid detection system 1 being integrated into nucleic acid draw-out device 3 can save use space and save user's
Operating time.
Please continue to refer to Fig. 4 and cooperate Fig. 5.In Fig. 4 and Fig. 5, nucleic acid draw-out device 3 include at least driving platform 32,
The piston platform 34 for putting injection ram 342, the pipette tips disk 36 for putting pipette tips 362, accommodating have splendid attire purified to test sample
The rack for test tube to be measured 38 of the test tube 382 to be measured of more of product and pushing unit 39 with drive motor 392, wherein driving is flat
Platform 32 can drive storing to have the piston platform 34 of injection ram 342 on entire pedestal 31, with upper and lower (Y-axis side in such as Fig. 4
To) vertical direction is mobile, left and right direction (X-direction in such as Fig. 4) is mobile or front and rear direction (Z-direction in such as Fig. 4) is moved
It is dynamic.In addition, the pushing unit 39 with drive motor 392 is arranged in below the pipette tips disk 36 for putting pipette tips 362, and in pipette tips disk
36 lower sections are moved back and forth with X-direction, and according to the design of firmware, allow pushing unit 39 that can be moved to the pipette tips for being intended to pushing tow
362 lower section, therefore pushing unit 39 can be moved to the lower sections of the default pipette tips 362 for wanting pushing tow in X-direction, reach positioning
Later, pushing unit 39 is controlled towards Y direction by drive motor 392 rise again, while when top, heap unit 39 is ramped up,
Also will push up to preset above heap unit 39 wants the pipette tips 362 of pushing tow to be ejected by pipette tips disk 36 towards the direction of injection ram 342, and this
When driving platform 32 drive to put and have that the piston platform 34 of injection ram 342 is mobile with the direction of Z-direction towards pipette tips disk 36,
And in alignment with 362 top of pipette tips jacked up, injection ram 342 is combined with pipette tips 362 downwards (Y direction), due to injection
The piston head (not shown in the figure) of piston 342 is also equipped with sealing ring (not shown in the figure), so that pipette tips 362 and injection are lived
Plug 342 can combine closely after combining by sealing ring.After injection ram 342 is combined with pipette tips 362, platform is driven
32 drive the injection ram combined with pipette tips 362 342 to be moved on rack for test tube 38 to be measured again, at this point, driving platform 32 controls rifle
First 362 move in Y direction, the test tube to be measured 382 of the direction sample to be tested to be detected, and by 382 interior suction of test tube to be measured
Take suitable sample to be tested.
And the optical detection apparatus 4 of nucleic acid detection system 1 is extracted automatically at least by with light-measuring mechanism 42, the tested area of sample
44, arithmetic element 46, control unit 48, cleansing tissue 50, memory unit 52 and display unit 54 are formed, wherein light-measuring mechanism
42 have included at least survey radiant end 422, filter (not shown in the figure) or monochromator (not shown in the figure) and detection system
The tested area 44 of (not shown in the figure), sample, signal designation system (not shown in the figure) and pivot end 424 are constituted.It says
Bright, optical detection apparatus 4 is the suction brightness for being generally commonly used to measure sample to be tested, then by the suction brightness benefit measured
It can be further calculated in sample to be tested with arithmetic element 46, the quantitative analysis device of nucleic acid or protein concentration, because
This optical detection apparatus 4 can be micro light splitting luminance meter or ultramicron light splitting luminance meter.And it surveys in radiant end 422 at least
It include the optics spare part such as filter or monochromator (not shown in the figure), in addition, the detection system in optical detection apparatus 4
The tested area 44 of (not shown in the figure), sample, signal designation system (not shown in the figure) and pivot end 424 with survey radiant
End 422 is connected in a manner of mechanical or electrical property, for the well known spare part for constituting light-measuring mechanism 42, therefore above-mentioned each component
Connection type, function or performance it is identical as existing light-measuring mechanism 42, also and non-present invention technology emphasis, therefore, no
Add to state herein.
Only it should be particularly noted that, light-measuring mechanism 42 can be optical fiber shaft in the present invention, therefore in optical fiber shaft
One end be to survey radiant end 422, and the other end is that pivot end 424 is articulated on the ontology of optical detection apparatus 4, can be by
The moving in rotation with the shaft of ontology pivot joint, therefore, optical detection apparatus 4 launch light source through surveying radiant end 422, and shine
Penetrate the purified sample to be tested in the tested area 44 of sample.In the present invention, the light source emitted by survey radiant end 422
The predominantly light source of high-energy, and the full spectrum that wavelength is 220nm-750nm can be provided and can be used as optical detection apparatus 4
Light source.
The tested area 44 of the sample of optical detection apparatus 4 is to accommodate sample to be tested, and in the present invention, the tested area 44 of sample is
Arc groove, convenient for sample to be tested can be placed in completely in the tested area 44 of sample, without towards except the tested area 44 of sample and
It overflows, and after suitable sample to be tested such as 1ul-2ul instillation sample tested area 44, due to the surface tension of liquid, makes
Obtaining liquid will form fluid column in the tested area 44 of sample and is placed in the tested area 44 of sample, since sample to be tested is because of surface
Power and form fluid column in the tested area 44 of sample, with traditional light splitting luminance meter using cuvette by solution to be measured be placed in wherein and
Measure is the same principle, when surveying radiant irradiation fluid column, the suction brightness of the available sample to be tested.
In an embodiment of the present invention, optical detection apparatus 4 further includes control unit 48, is connect with light-measuring mechanism 42, control
Unit 48 processed is radiated at the tested area 44 of sample mainly to control light-measuring mechanism 42, through the transmitting light source of radiant end 422 is surveyed
On sample to be tested, and after sample to be tested completes irradiation, the data transmission that will test to arithmetic element 46, and by fortune
Calculating unit 46 will test the data conversion of the sample to be tested into suction brightness and the concentration of corresponding nucleic acid and/or albumen,
And simultaneously by above-mentioned testing result output optical detection apparatus 4 display unit 54 or output to it is of the invention automatic
The external device (ED) 6 that nucleic acid detection system 1 connects is extracted to be shown or stored come the data that will test.
In addition, control unit 48 also controls the optical fiber shaft on optical detection apparatus 4 after sample to be tested is completed to detect
The pivot end 424 of (not shown in the figure) is rotated, tested in sample in the cleansing tissue 50 surveyed on radiant end 422 to drive
Area 44 is upper and lower and left and right direction is mobile, and when the pivot end 424 of optical fiber shaft drives cleansing tissue 50 when moving up and down, this is dynamic
Work is in order to draw the liquid in the tested area 44 of sample, when the pivot end 424 of optical fiber shaft drives cleansing tissue 50 to move left and right
When, this movement be in order to which the further wiped clean of liquid not being drawn completely in the tested area 44 of sample will be remained in, with
Achieve the purpose that automatic erasing.Whereby, it can be ensured that the metric data of next sample product to be measured will not be by previous liquid institute
Pollute and influence the correctness of data.
Optical detection apparatus 4 further includes having memory unit 50, is electrically connected with arithmetic element 46, and light light is surveyed in mainly storage
Obtained data after source 422 is irradiated sample to be tested (not shown in the figure) and calculated via arithmetic element 46.In addition to this,
Memory unit 46 can also store other using parameter, for example, user set when in use the detection date, test sample name
Claim and/or with reference to brightness-concentration (blank absorbance-concentration) data etc. are inhaled, therefore, user can be saturating
User interface (not shown in the figure) is crossed to check the data of current each sample to be tested detected and be stored in memory
Other parameters or data in unit 50.
In addition, optical detection apparatus 4 further includes display unit 52, it is used to that the suction of obtained sample to be tested will to be measured at present
Brightness and/or than for reference inhale brightness-concentration (blank absorbance-concentration) data it is obtained to
The concentration of sample is shown, allows user that can obtain metric data in real time.In addition, user can penetrate user interface, it will
Brightness-concentration data is inhaled in detection date, test sample title and/or the reference being stored in memory unit 50, and is shown simultaneously
In on display unit 52.
At this to illustrate that, there are on cleansing tissue 50 disclosed in the present invention multiple cleaning blocks, and each
The size of cleaning block is greater than the size in the tested area 44 of sample, this purpose is the clear area using larger area
Block may insure that the liquid in the tested area 44 of sample can be adsorbed completely and be removed, and each on cleansing tissue 50
The pivot end 424 that the size of cleaning block can correspond to light-measuring mechanism 42 drives when rotating surveys the angle that radiant end 422 rotates
Size.For example, cleansing tissue 50 is circle, rotates an angle with the pivot end 424 of light-measuring mechanism 42 as 10 degree, then wipes
The rotation size of the pivot end 424 of each cleaning corresponding light-measuring mechanism 42 of block on paper 50 is exactly 10 degree, then wipes at this time
Just there is 360/10=36 cleaning block in wipe paper 50, after cleaning block all uses on cleansing tissue 50, user
The cleansing tissue 50 that directly can be more renewed by removing the cleansing tissue 50 used on survey radiant end 422, whereby can be with
The tested area 44 of sample is cleaned, by the mode of automation to save manpower.
In another embodiment, cleansing tissue 50 can be further distinguished into the cleaning block of the multiple of 180 equal parts or 180 equal parts
The area of (not shown in the figure), each cleaning block is greater than the area in the tested area 44 of sample, and its purpose is can be true
Each the cleaning block for protecting cleansing tissue 50 has enough areas, by tested 44 wiped clean of area of sample.In addition, in this hair
In bright embodiment, cleansing tissue 50 can be lens paper or blotting paper, and the purpose is to the water imbibitions using cleansing tissue 50, utilize
Cleansing tissue 50 is adsorbed on the sample to be tested that measurement is completed in the tested area 44 of sample towards the tested area 44 of sample, and by sample
Tested 44 wiped clean of area whereby, can have liquid so that next sample to be tested is measured to avoid previous sample to be tested
Body remains in the tested area 44 of sample, causes subsequent measurement inaccuracy.
Then, Fig. 6 is please referred to.Fig. 6 is the step flow chart for indicating to extract nucleic acid detection system automatically.In Fig. 6, be by
Purified sample to be tested is extracted using mentioned-above nucleic acid draw-out device 3, and is detected via optical detection apparatus 4
The concentration of its sample to be tested and wiped using cleansing tissue 50 (as shown in Figure 5) purifying on optical detection apparatus 4 to
The entire automatic operation steps flow chart of sample also cooperates Fig. 4 to illustrate together simultaneously in the step process of explanatory diagram 6.
And the purification step of specimen solution is then as disclosed in the step process of Fig. 2, it is to be noted that, it is to be directed in this steps flow chart
After purifying, the step process for being extracted and being detected via the sample to be tested after preservation, rather than be directed to and do not purify
Sample to be tested.
Step 70, the test tube to be measured for being loaded with purified sample to be tested is seated on rack for test tube to be measured.In this step
In 70, purified sample to be tested can be optionally stored under conditions of low temperature or room temperature, when being tested and analyzed
When, then the test tube to be measured 382 for being loaded with purified sample to be tested is taken out and is placed on rack for test tube 38 to be measured.Then step
72, pushing unit, which is moved to, to be intended to below pipette tips to be used, and by pipette tips by ejecting upwards on pipette tips disk.Step 74, it drives
Platform driving injection ram is moved to above the pipette tips jacked up and in conjunction with pipette tips.In above-mentioned steps 72 and step 74,
Pushing unit 39 can be according to setting, and be arranged on pipette tips disk 36 default is moved to by drive motor 392 will be by pushing tow
362 lower section of pipette tips, then again jacks up pipette tips 362 towards Y direction upwards.
For example, by taking Fig. 4 as an example, it is a that the pipette tips 362 in the left side of the drawing of Fig. 4, which can number, therefore by drawing
The pipette tips 362 on left side to right side are a, b, c ..., p totally ten six roots of sensation pipette tips according to number, are opened when extracting nucleic acid detection system 1 automatically
When beginning to operate, pushing unit 39 can be moved to the lower section for the pipette tips 362 that number is 1 to the left side (X-direction) of drawing, then,
The pipette tips 362 of number 1 can be ejected pipette tips disk 36 upwards (Y direction) by pushing unit 39, and the driving of platform 32 is driven to put at this time
It is mobile with Z-direction there is the piston platform 34 of injection ram 342, and is moved in the pipette tips 362 that the number jacked up is a
Side, driving platform 32 drive the injection ram 342 in piston platform 34 to move in Y direction towards 362 direction of pipette tips that number is a
It is dynamic, so that the pipette tips 362 of call number a and injection ram 342 is combined closely and is left pipette tips disk 36, and make injection ram 342
There is good air-tightness between pipette tips 362.
In another embodiment of the present invention, above-mentioned steps 72 and step 74 can be exchanged, therefore after step is exchanged, and be driven
Moving platform 32 first drives the piston platform 34 with injection ram 342 to be moved to pipette tips disk 36 and numbers the upper of the pipette tips 362 for being a
Side, pushing unit 39 are moved to the lower section for the pipette tips 362 that the number of default is a again, recycle the motor 392 of pushing unit 39
Pushing unit 39 is controlled, with the direction rising for the pipette tips 362 that Y direction is a towards preset number, until will number as a's
After pipette tips 362 eject pipette tips disk 36, driving platform 32 drives the pipette tips that injection ram 342 is a towards number in Y direction again
362 move down, until contacting and combining closely with the pipette tips 362 that number is a.
Then step 76, injection ram draw invisible spectro purified sample to be tested to be measured, and by it is purified to
Sample, which is tested and analyzed, obtains the data of purified sample to be tested.In this step, in injection ram 342 and pipette tips
After 362 combine, with Z-direction, drive platform 32 that injection ram 342 and pipette tips 362 are moved to 38 top of rack for test tube to be measured,
And it is moved to 382 top of test tube to be measured for being loaded with the purified sample to be tested to be detected, then drives platform 32 with Y-axis
Direction piston platform 34 is moved down in Y direction, so that the injection ram 342 with pipette tips 362 is simultaneously with Y direction direction
382 direction of test tube to be measured on rack for test tube 38 to be measured is mobile, and allows the pipette tips 362 on injection ram 342 to be downwardly into and be loaded with
In the test tube to be measured 382 of purified sample to be tested, and purified sample to be tested is drawn by test tube 382 to be measured to pipette tips
In 362, after the purified sample to be tested for drawing certain volume such as 1ul-2ul, driving platform 32 will have pipette tips again
362 injection ram 342 is moved up towards Y direction, so that pipette tips 362 are far from rack for test tube 38 to be measured, and has pipette tips 362
Injection ram 342 will not be collided when moving with other test tubes 382 or component to be measured.
Optical detection apparatus 4 is then mobile towards left side (X-axis) direction of drawing by the right side of the drawing of current Fig. 4, when
Optical detection apparatus 4 is moved to after positioning, such as on the left of the drawing by being moved to Fig. 4 on the right side of the drawing of Fig. 4, drives platform
32 again by the injection ram 342 with pipette tips 362, in Z-direction movement and in alignment with the tested area of the sample of optical detection apparatus 4
After 44, injection ram 342 by the purified sample to be tested in pipette tips 362 it is suitable drop optical detection apparatus 4 sample
In tested area 44, is launched using the survey radiant end 422 of optical detection apparatus 4 and survey radiant to detect in the tested area of sample
Purified sample to be tested on 44, and obtain the suction brightness of purified sample to be tested, then will inhale via arithmetic element 46 bright
Degree is converted into concentration, it can obtains the nucleic acid or protein concentration in purified sample to be tested.
In another embodiment of the present invention, when there was only an injection ram 342 in piston platform 34, platform is driven
32 can be moved on rack for test tube 38 to be measured with Z-direction in the injection ram 342 with pipette tips 362 and be drawn in sample tube
After purified sample to be tested in 382, the injection ram 342 with pipette tips 362 first with Y direction move upwardly away to
Pipe support 38 is tested, then is returned with the Z-direction moved originally, and is mobile towards optical detection apparatus 4 with X-direction again, directly
Until tested 44 top of area of the sample that the injection ram 342 with pipette tips 362 is moved to optical detection apparatus 4.The present embodiment
In, it is only necessary to the mobile injection ram 342 with pipette tips 362 is without optical detection apparatus 4, therefore no matter nucleic acid extracts dress
Setting the injection ram 342 being arranged on 3 platform is one or more, can be by base of the driving platform 32 in rack 11
It is moved on platform 31.
Then step 78, display/output test result.In this step, the detection and analysis of abovementioned steps 76 are obtained
The suction brightness or concentration of the sample to be tested of purifying are shown on the display panel by optical detection apparatus 4, or output
It is printed to such as printer of external device (ED) 6 or is stored such as laptop, Desktop Computing using storage device
Machine or USB are transmitted to running gear by wireless wired mode or server are stored.
In addition, after the step 76 other than it will test result using step 78 and exported or shown, in step
79, cleansing tissue carries out clean wiping to the tested area of the sample of optical detection apparatus and had been detected in the tested area of sample with removing
Sample to be tested.In step 79, after completing detection, the pivot end 424 that control unit 48 can control light-measuring mechanism 42 is rotated
To drive survey radiant end 422 to move up and down and turn left and right, so that surveying light light when the pivot end 424 of light-measuring mechanism 42 drives
When source 422 moves up and down, the entire area of one of cleaning block on the cleansing tissue 50 surveyed on radiant end 422
It can be contacted towards the tested area 44 of sample with to the tested area 44 of sample for accommodating purified sample to be tested, it will by capillary phenomenon
In the tested area 44 of sample, detected and purified sample to be tested draw, then when 424 band of pivot end of light-measuring mechanism 42
It is dynamic survey radiant end 422 it is left and right mobile when, remaining in the tested area 44 of sample will further detect and
The sample to be tested wiped clean of purifying, therefore cleansing tissue 50 is that complete simulation wipes sample manually in the tested area 44 of sample
The movement in the tested area 44 of product carries out, and can clean the tested area 44 of sample by the mode of automation whereby, in addition to saving people
It, can be to avoid the careless mistake in manual operation except power.
For example, when the angle that the pivot end 424 of light-measuring mechanism 42 drives survey radiant end 422 to rotate each time is 10
Degree, can be divided into 360/10 for circular cleansing tissue 50, totally 36 equal portions, the area of each equal portions are greater than the tested area of sample
44, and the pivot end 424 of light-measuring mechanism 42 will be wiped in a manner of left and right in the range of this 10 degree to remove in sample
Detection is had been subjected in tested area 44.And purified sample to be tested, it is purified to be measured in the tested area 44 of sample to ensure
Sample can be cleaned completely by cleansing tissue 50 and be removed, and when to carry out the cleaning tested area 44 of sample next time, light-measuring mechanism
42 pivot end 424 will be rotated further by next 10 degree of angles, so that also going to simultaneously in the cleansing tissue 50 surveyed on radiant end 422
Next cleaning block, so that cleaning sample the cleansing tissue 50 surveyed on radiant end 422 can be clean cleaning block
The tested area of product.And in the present invention, the beginning and end on cleansing tissue 50 can do color marking, i.e., when all clear areas
After block all uses, user can learn by the prompting of color marking needs replacing cleansing tissue 50.
In addition, survey the top of radiant end 422 since cleansing tissue 50 can be arranged directly on, replacement is convenient, can be complete
Purified sample to be tested of the removal in the tested area 44 of sample, will not allowing the tested area 44 of sample, there are also previous to test sample
The Liquid Residue of product and the detection accuracy for influencing next purified sample to be tested.
Herein it is noted that after the completion of above-mentioned steps 76, other than directly carrying out step 78, in step 80, drive
Injection ram with pipette tips is moved to original standby position by moving platform, and pipette tips are expelled in discarded slot and are recycled.
In this step, since the injection ram 342 with pipette tips 362 has dripped purified sample to be tested in optics in step 76
It is analyzed in detection device 4, this pipette tips 362 cannot reuse, also for the difficulty for avoiding cross contamination and cleaning,
The pressure that outside pushing is granted by injection ram 342 (is not scheming pipette tips 362 by being exited on injection ram 342 to discarded slot
Middle expression) it abandons.
In addition, Fig. 7 is the step flow chart for indicating to extract another embodiment of nucleic acid detection system automatically.Fig. 7's and Fig. 6
Difference is, Fig. 7 be for an also unpurified specimen to be measured using disclosed herein automatic extraction nucleic acid detection system into
Capable purifying is extracted, and the concentration and utilization cleansing tissue 52 that detect its specimen to be measured via optical detection apparatus 4 are (such as Fig. 5 institute
Show) wipe the entire automatic operation steps flow chart of the sample to be tested on optical detection apparatus 4, explanatory diagram 7 the step of
In process, also Fig. 4 is cooperated to illustrate together simultaneously.It is noted that being for also without purifying in this steps flow chart
Specimen solution starts to illustrate.
Step 900, the reagent strip for being loaded with magnetic bead and specimen solution to be purified is provided, and is arranged and is put in reagent strip
On platform.It in this step, can be according to the quantity for the specimen solution of user to be purified, to contain magnetic bead in reagent strip 2
Quantity in test tube.For example, if user's specimen solution to be purified is 16 groups, there will be 16 in reagent strip 2
Test tube contains magnetic bead and specimen solution.
Step 901, the cracking liquid bath for being equipped with lysate is provided.Then, step 902, by injection pipette tips in conjunction with pipette tips.
In this step 902, Z-direction of the platform 32 in such as Fig. 4 is driven, the injection ram 342 in piston platform 34 is moved to
On pipette tips disk 36, then drives platform 32 in the movement of pipette tips 362 by piston platform 34 with Y direction towards pipette tips disk 36, allow
Injection ram 342 can be combined with pipette tips 362, and the top end unit 39 below pipette tips disk 36 is arranged in herein to be acted, meaning
Refer to, the combination of injection ram 342 and pipette tips 362 at this time is not needed pipette tips 362 using pushing unit 39 by pushing up on pipette tips disk 36
It rises.Because in purification step, depending on the quantity of user's specimen solution to be purified, drive platform 32 that will allow how many
Injection ram 342 is combined with pipette tips 362, if if the aforementioned user specimen solution to be detected is 16 groups, in injection ram 342
Quantity with pipette tips 362 is then respectively 16.Also due to it is that the pipette tips 362 used, which are not particularly limited, therefore
Do not need movable top heap unit 39 to it is preset that pipette tips 362 lower section.
Following step 903 draws lysate using the injection ram with pipette tips and is loaded with lysate merging
In the reagent strip of magnetic bead and specimen solution to be purified, lysate and specimen solution reaction to be purified is allowed to form mixture, and
Magnetic bead is allowed to adsorb the nucleic acid in mixed liquor.It is identical as the step 20 in Fig. 2 in this step 903, using lysate and break to
The specimen solution of purifying is mixed, it may also be necessary to will be loaded with the examination of magnetic bead, specimen solution to be purified and lysate
Agent item 2 is placed in heating tank (not shown in the figure), under conditions of water temperature is 50 DEG C -56 DEG C, water bath time is 10-15 minutes
It carries out.In another embodiment, in order to which lysate and specimen solution to be purified to be mixed uniformly, it can also will be loaded with
The reagent strip 2 of magnetic bead, specimen solution to be purified and lysate is placed in ultrasonic oscillator, also controls water temperature
50 DEG C -56 DEG C, water bath time be 10-15 minutes, and vibrated, specimen solution and lysate to be purified accelerated
Reaction, and allow the cell of specimen solution to be purified to be cleaved liquid and break, and the nucleic acid in cell can be released in mixed liquor
It is interior, the nucleic acid being dissolved in mixed liquor is adsorbed by magnetic bead again.
Step 904, magnetic bead is separated with mixed liquor.In this step 904, driving platform 32 will be equipped with reagent strip 21
Before reagent strip placement platform 2 is moved to magnetic attracting device, each magnetic attracting device is aligned and close to each of reagent strip 21
A test tube.When tube wall of the magnetic attracting device close to test tube, on the tube wall that magnetic bead can be attracted to test tube because of magnetic force, magnetic bead at this time
It is separated with mixed liquor, also means nucleic acid because being adsorbed and being adsorbed on the tube wall of test tube by magnetic attracting device, then nucleic acid by magnetic bead
It is separated with mixed liquor.At this point, there is the injection ram 342 of pipette tips to protrude into reagent strip 21 in Y direction for the driving driving of platform 32
In each test tube, by each invisible spectro complete extinction of mixed liquor, and by removing in test tube, this mixed liquor is not include core
The waste liquid of acid, and drive platform 32 that absorption is driven to have the injection ram 342 of the pipette tips 362 of mixed liquor to be moved to discarded slot and (do not exist
Indicated in figure), the mixed liquor in pipette tips 362 is all discharged into discarded slot.Herein it is noted that pipette tips in the present invention
Be smooth surface in the design of 362 inner wall, have the function of water-sprinkling, that is to say, that when pipette tips 362 by mixed liquor by pipette tips
When being expelled to discarded slot in 362, any mixed liquor is not had and is remained on the inner pipe wall of pipette tips 362.
And then, step 905, magnetic bead is cleaned using cleaning solution, cleaning is not the other impurities of nucleic acid on magnetic bead, and will
Other impurities on magnetic bead by removing.In abovementioned steps 904, when mixed liquor via being removed in test tube when, it is only remaining in test tube
It is adsorbed with the magnetic bead of nucleic acid, at this time the test tube tube wall by magnetic attracting device far from reagent strip 21, so that between magnetic bead and magnetic attracting device
Attraction disappear, and magnetic bead can be fallen in vitro.Meanwhile in abovementioned steps 904, injection ram 342 is by mixed liquor by rifle
First 362 when being expelled to discarded slot, and also pipette tips 362 are expelled to together in discarded slot simultaneously,
Step 906, the nucleic acid on magnetic bead is removed using eluent, to form purified sample to be tested.And it injects and lives
Plug 342 repeats abovementioned steps 902 and is combined with clean pipette tips 362, then draws cleaning solution and later there was only cleaning solution merging
In the test tube of magnetic bead.Be mixed to magnetic bead and cleaning solution and then by magnetic attracting device close to test tube tube wall to adsorb magnetic bead,
So that magnetic bead is separated with cleaning waste liquid.Be to clean on magnetic bead using the purpose of cleaning solution herein be not nucleic acid other are miscellaneous
Matter, and by other impurities by being removed on magnetic bead, and cleaning solution can be ethyl alcohol.
Likewise, with abovementioned steps 904, the step of magnetic bead is separated with mixed liquor, is identical, and the injection with pipette tips 362 is living
Plug 342 is discharged into after drawing invisible spectro cleaning waste liquid to discarded slot, and pipette tips 362 are expelled to discarded slot simultaneously
In, and after the injection ram 342 with pipette tips 362 has removed invisible spectro cleaning waste liquid, by magnetic attracting device far from reagent
The test tube tube wall of item 21, so that the attraction between magnetic bead and magnetic attracting device disappears, and magnetic bead can be fallen in vitro.Then, it infuses
Penetrate piston 342 repeat abovementioned steps 902 and clean pipette tips 362 in conjunction with and draw eluent, then eluent merging is had
In the test tube of magnetic bead, nucleic acid is dissolved in eluent by removing on magnetic bead using eluent, this eluent is as purified
Sample to be tested.Then, magnetic attracting device is recycled, to adsorb magnetic bead on tube wall, not have on magnetic bead at this time close to test tube tube wall
With the presence of any nucleic acid.Then, the injection ram 342 with pipette tips 362 draws purified sample to be tested in vitro,
And drive the driving of platform 32 that there is the injection ram 342 of pipette tips 362 to be moved to the test tube to be measured for being equipped with more test tubes 382 to be measured
38 top of frame, each is directed at each test tube 382 to be measured with the injection ram 342 with pipette tips 362, then by pipette tips
Purified sample to be tested is expelled in test tube 382 to be measured in 362.Above-mentioned steps 900-906 be using disclosed herein
Automatic nucleic acid extraction system 1 the step of specimen solution is purified.
It is detected likewise, above-mentioned purified sample to be tested can directly carry out nucleic acid extraction, in step and earlier figures 6
Step 70-80 it is identical, herein step is repeated to state, but details is not just added to state.Step 907, it will be loaded with pure
The test tube to be measured of the sample to be tested of change is seated on rack for test tube to be measured.Step 908, pushing unit, which is moved to, is intended to pipette tips to be used
Lower section, and by pipette tips by being ejected upwards on pipette tips disk；Step 909, pushing unit, which is moved to, is intended to below pipette tips to be used, and
And by pipette tips by being ejected upwards on pipette tips disk；Step 909, driving platform driving injection ram is moved to above the pipette tips jacked up
And in conjunction with pipette tips；Step 910, injection ram draws invisible spectro purified sample to be tested to be measured, and will purify
Sample to be tested tested and analyzed, and obtain the data of purified sample to be tested；Step 912, display/output detection knot
Fruit.Likewise, after step 910 other than it will test result using step 912 and exported or shown, in step
913, cleansing tissue carries out clean wiping to the tested area of the sample of optical detection apparatus and had been detected in the tested area of sample with removing
Sample to be tested.After the completion of above-mentioned steps 910, other than directly carrying out step 912, in step 911, drive platform will
Injection ram with pipette tips is moved to original standby position, and pipette tips are expelled in discarded slot and are recycled.
The foregoing is merely the preferred embodiments of the invention, the interest field that is not intended to limit the invention；More than simultaneously
Description, the special personage of correlative technology field should can be illustrated and be implemented, thus other without departing from it is disclosed it
The lower equivalent change or modification completed of spirit, should be included in claim.
Claims (10)
1. a kind of automatic nucleic acid extract detection system, characterized by comprising:
Nucleic acid draw-out device includes:
Pedestal；
Platform is driven, driving platform front and back on the pedestal moves back and forth and moves left and right；
More injection rams, each injection ram are arranged in piston platform, and each injection ram is in the piston
It is moved up and down on platform with vertical direction；
Rack for test tube to be measured has multiple holding holes；
More test tubes to be measured, to contain purified sample to be tested, each test tube to be measured is arranged in the test tube to be measured
In each holding hole of frame；
More pipette tips, each pipette tips are arranged on pipette tips disk；And
Pushing unit is arranged below the pipette tips disk, and the pushing unit has drive motor, and the drive motor drives institute
It states pushing unit to move back and forth and can be positioned to the pipette tips to be jacked up below the pipette tips disk, by the pushing tow
Unit in a manner of moving up and down by the pipette tips of top by the pipette tips disk towards the injection ram direction eject,
And allow the injection ram with the pipette tips in conjunction with the injection ram；
Optical detection apparatus, is set to the lower section of the driving platform, and the optical detection apparatus includes:
The tested area of sample, to accommodate the purified sample to be tested；
Light-measuring mechanism has and surveys radiant end and pivot end, and the ontology of the pivot end and the optical detection apparatus is pivotally connected, institute
It states and surveys radiant end to emit light source；
Cleansing tissue has multiple cleaning blocks, and the cleansing tissue is arranged on the survey radiant end；And
Control unit emits the light source through the survey radiant end to control the light-measuring mechanism；
Whereby, the injection ram with the pipette tips is moved on the rack for test tube to be measured wherein by the driving platform
Above one test tube to be measured, the injection ram of the driving platform driving with the pipette tips towards described in having
The in-pipe to be tested of the sample to be tested of purifying, it is described purified to test sample in the test tube to be measured to draw
Product are simultaneously placed in the pipette tips, the driving platform by drawn the purified sample to be tested with the pipette tips
The injection ram moves up, so that the separate test tube to be measured with the purified sample to be tested of the pipette tips,
Drive platform that the injection ram with the pipette tips for having drawn the purified sample to be tested is moved to the light
In the tested area of the sample for learning detection device, the injection ram drips the purified specimen to be measured in the pipette tips
In the tested area of the sample, through described control unit control the light-measuring mechanism emit the light source with detect the sample by
The purified sample to be tested for surveying area obtains the detection data of the purified sample to be tested, the optical detection dress
The cleansing tissue of the described control unit and control set on the survey radiant end is drawn in the tested area of the sample
The purified sample to be tested, and by the tested area's wiped clean of the sample.
2. automatic nucleic acid as described in claim 1 extracts detection system, which is characterized in that further include reagent strip and put disk, institute
It states reagent strip and puts disk to put reagent strip.
3. automatic nucleic acid as claimed in claim 2 extracts detection system, which is characterized in that be loaded in the reagent strip impure
Change or a purified specimen to be measured.
4. a kind of automatic nucleic acid extract detection system, characterized by comprising:
Nucleic acid draw-out device includes:
Pedestal；
Reagent strip placing platform, to set at least one more reagent strip, and the reagent strip has multiple test tubes, and at least one examination
Purified sample to be tested is loaded in pipe；
Platform is driven, driving platform front and back on the pedestal moves back and forth and moves left and right；
More injection rams, each injection ram are arranged in piston platform, and each injection ram is in the piston
It is moved up and down on platform with vertical direction；
More pipette tips, each pipette tips are arranged on pipette tips disk；And
Pushing unit is arranged below the pipette tips disk, and the pushing unit has drive motor, and the drive motor drives institute
It states pushing unit to move back and forth and can be positioned to the pipette tips to be jacked up below the pipette tips disk, by the pushing tow
Unit in a manner of moving up and down by the pipette tips of top by the pipette tips disk towards the injection ram direction eject,
And allow the injection ram in conjunction with the pipette tips jacked up on the pipette tips disk；
Optical detection apparatus, is set to the lower section of the driving platform, and the optical detection apparatus includes:
The tested area of sample, to accommodate the purified sample to be tested；
Light-measuring mechanism has and surveys radiant end and pivot end, and the ontology of the pivot end and the optical detection apparatus is pivotally connected, institute
It states and surveys radiant end to emit light source；
Cleansing tissue has multiple cleaning blocks, and the cleansing tissue is arranged on the survey radiant end；And
Control unit emits the light source through the survey radiant end to control the light-measuring mechanism；
Whereby, the injection ram with the pipette tips is moved in the reagent strip with described in by the driving platform
Above the test tube of the sample to be tested of purifying, the driving platform driving has the injection ram of the pipette tips towards tool
There is the examination in-pipe of the purified sample to be tested, to draw with described in the purified sample to be tested
The invisible spectro purified sample to be tested is simultaneously placed in the pipette tips, and the driving platform will draw described purified
The injection ram with the pipette tips of sample to be tested move up so that the pipette tips far from it is described it is purified to
The test tube of sample, and the injection ram with the pipette tips for having drawn the purified sample to be tested is moved
It moves to the tested area of the sample of the optical detection apparatus, the injection ram will be described purified in the pipette tips
Sample to be tested drop controls the light-measuring mechanism through described control unit and emits the light source to detect in the tested area of the sample
Purified sample to be tested is stated, and obtains the detection data of the purified sample to be tested, the institute of the optical detection apparatus
State control unit control it is described survey radiant end on the cleansing tissue draw in the tested area of the sample described in it is pure
The sample to be tested of change, and by the tested area's wiped clean of the sample.
5. automatic nucleic acid as claimed in claim 4 extracts detection system, which is characterized in that further include rack for test tube to be measured to set
It puts with more test tubes to be measured for containing purified sample to be tested.
6. automatic nucleic acid as described in claim 1 or 4 extracts detection system, which is characterized in that further include magnetic attracting device setting
In the lower section of the driving platform, and after the piston apparatus is in conjunction with the pipette tips, it is directed at each pipette tips
The position of gun barrel.
7. automatic nucleic acid as described in claim 1 or 4 extracts detection system, which is characterized in that external device (ED) is further included, it is described
External device (ED) with to export or store passed by the described control unit of the optical detection apparatus it is described purified
The detection data of sample to be tested.
8. nucleic acid as claimed in claim 7 automatic extracts detection system, which is characterized in that the external device (ED) be printer,
Server, external storage device or running gear.
9. automatic nucleic acid as described in claim 1 or 4 extracts detection system, which is characterized in that discarded slot is further included, it is described useless
Slot is abandoned to drip the purified sample to be tested in the tested area of the sample via the pipette tips to store the injection ram
Later, the pipette tips by being exited on the injection ram.
10. as claim 1 or 4 or the automatic nucleic acid extract detection system, which is characterized in that further include heating tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810312677.7A CN110387322A (en) | 2018-04-09 | 2018-04-09 | Automatic nucleic acid extracts detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810312677.7A CN110387322A (en) | 2018-04-09 | 2018-04-09 | Automatic nucleic acid extracts detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110387322A true CN110387322A (en) | 2019-10-29 |
Family
ID=68282561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810312677.7A Pending CN110387322A (en) | 2018-04-09 | 2018-04-09 | Automatic nucleic acid extracts detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110387322A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112565304A (en) * | 2021-02-18 | 2021-03-26 | 北京声智科技有限公司 | Equipment management method and device and electronic equipment |
| CN113444625A (en) * | 2020-03-27 | 2021-09-28 | 大江生医股份有限公司 | Automated nucleic acid detection system and method |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101336370A (en) * | 2006-04-03 | 2008-12-31 | 株式会社岛津制作所 | Optical measurement apparatus for trace liquid sample |
| DE202013102057U1 (en) * | 2013-05-10 | 2013-06-06 | Rbc Bioscience Corp. | Device for extracting nucleic acids by means of magnetic beads |
| CN203017805U (en) * | 2012-08-07 | 2013-06-26 | 芮宝生医股份有限公司 | Magnetic bead type nucleic acid extraction equipment |
| TW201331359A (en) * | 2012-01-20 | 2013-08-01 | Zinexts Life Science Corp | Nucleic acid extraction method |
| CN105067806A (en) * | 2015-08-03 | 2015-11-18 | 瑞莱生物科技（江苏）有限公司 | Full-automatic immunofluorescence detection device |
| CN105543089A (en) * | 2016-03-08 | 2016-05-04 | 湖南圣湘生物科技有限公司 | Magnetic bead based nucleic acid extraction device and method |
| CN205833187U (en) * | 2016-07-18 | 2016-12-28 | 黑龙江省野生动物研究所 | For placing the rifle head cartridge of liquid transfer gun head |
| US20170082616A1 (en) * | 2014-12-29 | 2017-03-23 | Getein Biotech, Inc. | Fully-automatic immunofluorescence quantitative analysis apparatus and detection method |
| CN107287092A (en) * | 2017-08-16 | 2017-10-24 | 湖南圣湘生物科技有限公司 | Nucleic acid extraction instrument |
| CN206583802U (en) * | 2017-02-14 | 2017-10-24 | 北京源诚科仪生物技术有限公司 | Miniature full-automatic chemical luminescence detector |
| CN109324002A (en) * | 2018-12-11 | 2019-02-12 | 宁波医杰生物科技有限公司 | A kind of full-automatic detector |
| CN208537027U (en) * | 2018-04-09 | 2019-02-22 | 恺硕生物科技(厦门)有限公司 | Automatic erasing is divided brightness measurement system |
| CN110408610A (en) * | 2018-04-27 | 2019-11-05 | 恺硕生物科技(厦门)有限公司 | Nucleic acid extracting reagent item |
| CN110551712A (en) * | 2018-06-04 | 2019-12-10 | 恺硕生物科技(厦门)有限公司 | Nucleic acid extraction machine table with gun head positioning device |
| CN210142066U (en) * | 2019-06-19 | 2020-03-13 | 佛山市艾达思精密仪器有限公司 | Automatic sample adding and cleaning trace sample detection device assembly |
-
2018
- 2018-04-09 CN CN201810312677.7A patent/CN110387322A/en active Pending
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101336370A (en) * | 2006-04-03 | 2008-12-31 | 株式会社岛津制作所 | Optical measurement apparatus for trace liquid sample |
| TW201331359A (en) * | 2012-01-20 | 2013-08-01 | Zinexts Life Science Corp | Nucleic acid extraction method |
| CN203017805U (en) * | 2012-08-07 | 2013-06-26 | 芮宝生医股份有限公司 | Magnetic bead type nucleic acid extraction equipment |
| DE202013102057U1 (en) * | 2013-05-10 | 2013-06-06 | Rbc Bioscience Corp. | Device for extracting nucleic acids by means of magnetic beads |
| US20170082616A1 (en) * | 2014-12-29 | 2017-03-23 | Getein Biotech, Inc. | Fully-automatic immunofluorescence quantitative analysis apparatus and detection method |
| CN105067806A (en) * | 2015-08-03 | 2015-11-18 | 瑞莱生物科技（江苏）有限公司 | Full-automatic immunofluorescence detection device |
| CN105543089A (en) * | 2016-03-08 | 2016-05-04 | 湖南圣湘生物科技有限公司 | Magnetic bead based nucleic acid extraction device and method |
| CN205833187U (en) * | 2016-07-18 | 2016-12-28 | 黑龙江省野生动物研究所 | For placing the rifle head cartridge of liquid transfer gun head |
| CN206583802U (en) * | 2017-02-14 | 2017-10-24 | 北京源诚科仪生物技术有限公司 | Miniature full-automatic chemical luminescence detector |
| CN107287092A (en) * | 2017-08-16 | 2017-10-24 | 湖南圣湘生物科技有限公司 | Nucleic acid extraction instrument |
| CN208537027U (en) * | 2018-04-09 | 2019-02-22 | 恺硕生物科技(厦门)有限公司 | Automatic erasing is divided brightness measurement system |
| CN110408610A (en) * | 2018-04-27 | 2019-11-05 | 恺硕生物科技(厦门)有限公司 | Nucleic acid extracting reagent item |
| CN110551712A (en) * | 2018-06-04 | 2019-12-10 | 恺硕生物科技(厦门)有限公司 | Nucleic acid extraction machine table with gun head positioning device |
| CN109324002A (en) * | 2018-12-11 | 2019-02-12 | 宁波医杰生物科技有限公司 | A kind of full-automatic detector |
| CN210142066U (en) * | 2019-06-19 | 2020-03-13 | 佛山市艾达思精密仪器有限公司 | Automatic sample adding and cleaning trace sample detection device assembly |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113444625A (en) * | 2020-03-27 | 2021-09-28 | 大江生医股份有限公司 | Automated nucleic acid detection system and method |
| CN112565304A (en) * | 2021-02-18 | 2021-03-26 | 北京声智科技有限公司 | Equipment management method and device and electronic equipment |
| CN112565304B (en) * | 2021-02-18 | 2021-06-25 | 北京声智科技有限公司 | Equipment management method and device and electronic equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108660074B (en) | Integrated solution for nucleic acid extraction PCR amplification detection | |
| JP4959450B2 (en) | Chemical analyzer | |
| US20180134895A1 (en) | Composition for preventing evaporation of reaction solution during nucleic acid amplification reaction. | |
| CN1224500A (en) | Automatic chemistry analyzer | |
| JP2011518323A (en) | Assay devices, methods, and reagents | |
| JP2003507715A (en) | Liquid sample handling equipment | |
| CN109490559B (en) | Full-automatic chemiluminescence immunoassay instrument | |
| KR20150056479A (en) | Station for test device with integrated reaction and detection means | |
| CN110387322A (en) | Automatic nucleic acid extracts detection system | |
| JP2010538672A (en) | Method, apparatus and molecular biology kit for extracting amplified genetic material | |
| KR102403439B1 (en) | All-in-one real time pcr apparatus | |
| CN113125787A (en) | Full-automatic chemiluminescence immunoassay analyzer | |
| JP2016223919A (en) | Chip for chromatographic processing, chromatographic processing device, and chromatographic processing method | |
| JP6570522B2 (en) | In-vitro automatic diagnosis method | |
| JP5363604B2 (en) | Chemical analyzer | |
| CN108004233A (en) | A kind of negative pressure device available for automatic instrument for extracting nucleic acid | |
| KR102458032B1 (en) | Rt-pcr device | |
| JP2008157723A (en) | Blood quantity determiner | |
| TWI692377B (en) | Cap for microtube, kits and methods comprising the same | |
| CN210030647U (en) | Consumable and device for nucleic acid extraction | |
| JP6221206B2 (en) | Liquid filling device and method for filling liquid into capillary using the liquid filling device | |
| JP2005127895A (en) | Method for cleaning sampling tube in sampling mechanism of blood, or the like | |
| JP2016170075A (en) | Automatic analyzer and method for automatic analysis | |
| CN116008526B (en) | Microfluidic biomolecular chemiluminescence multi-joint detection device and multi-joint detection method | |
| CN109916817A (en) | A kind of liquid relief and analytical equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191029 |